CN114270167A - 血液检测方法及血液分析系统 - Google Patents
血液检测方法及血液分析系统 Download PDFInfo
- Publication number
- CN114270167A CN114270167A CN201980099668.7A CN201980099668A CN114270167A CN 114270167 A CN114270167 A CN 114270167A CN 201980099668 A CN201980099668 A CN 201980099668A CN 114270167 A CN114270167 A CN 114270167A
- Authority
- CN
- China
- Prior art keywords
- sample
- blood
- detected
- optical information
- intensity information
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 210000004369 blood Anatomy 0.000 title claims abstract description 182
- 239000008280 blood Substances 0.000 title claims abstract description 182
- 238000001514 detection method Methods 0.000 title claims abstract description 182
- 238000004159 blood analysis Methods 0.000 title claims abstract description 30
- 230000003287 optical effect Effects 0.000 claims abstract description 211
- 210000001772 blood platelet Anatomy 0.000 claims abstract description 164
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 103
- 210000001995 reticulocyte Anatomy 0.000 claims abstract description 99
- 238000000034 method Methods 0.000 claims abstract description 97
- 239000002245 particle Substances 0.000 claims abstract description 86
- 238000012360 testing method Methods 0.000 claims abstract description 72
- 210000003743 erythrocyte Anatomy 0.000 claims abstract description 56
- 239000003219 hemolytic agent Substances 0.000 claims abstract description 51
- 210000004027 cell Anatomy 0.000 claims description 61
- 210000000265 leukocyte Anatomy 0.000 claims description 58
- 239000000975 dye Substances 0.000 claims description 47
- 238000012545 processing Methods 0.000 claims description 36
- 239000007850 fluorescent dye Substances 0.000 claims description 35
- 150000007523 nucleic acids Chemical class 0.000 claims description 28
- 102000039446 nucleic acids Human genes 0.000 claims description 28
- 108020004707 nucleic acids Proteins 0.000 claims description 28
- 238000005070 sampling Methods 0.000 claims description 27
- 238000004590 computer program Methods 0.000 claims description 22
- 230000001413 cellular effect Effects 0.000 claims description 19
- 238000010586 diagram Methods 0.000 claims description 19
- 239000012634 fragment Substances 0.000 claims description 15
- 239000012528 membrane Substances 0.000 claims description 13
- 210000001616 monocyte Anatomy 0.000 claims description 13
- 210000000440 neutrophil Anatomy 0.000 claims description 13
- 238000002360 preparation method Methods 0.000 claims description 13
- 230000002438 mitochondrial effect Effects 0.000 claims description 12
- 210000004698 lymphocyte Anatomy 0.000 claims description 11
- 206010018910 Haemolysis Diseases 0.000 claims description 10
- 230000008588 hemolysis Effects 0.000 claims description 10
- 230000002159 abnormal effect Effects 0.000 claims description 9
- 239000003085 diluting agent Substances 0.000 claims description 8
- 239000012530 fluid Substances 0.000 claims description 8
- 210000003714 granulocyte Anatomy 0.000 claims description 6
- 238000000149 argon plasma sintering Methods 0.000 claims description 5
- 230000005856 abnormality Effects 0.000 claims description 3
- 230000001678 irradiating effect Effects 0.000 claims description 3
- 230000009969 flowable effect Effects 0.000 claims 1
- 239000000523 sample Substances 0.000 description 194
- 239000007788 liquid Substances 0.000 description 11
- -1 alkyl glycosides Chemical class 0.000 description 10
- 210000000601 blood cell Anatomy 0.000 description 9
- 229930182470 glycoside Natural products 0.000 description 9
- 230000009089 cytolysis Effects 0.000 description 8
- 150000001875 compounds Chemical class 0.000 description 7
- 238000005259 measurement Methods 0.000 description 6
- 239000000980 acid dye Substances 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 5
- 238000009534 blood test Methods 0.000 description 5
- 238000010186 staining Methods 0.000 description 5
- 239000003795 chemical substances by application Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 230000005284 excitation Effects 0.000 description 4
- 210000000207 lymphocyte subset Anatomy 0.000 description 4
- 150000007524 organic acids Chemical class 0.000 description 4
- 239000012488 sample solution Substances 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 210000003979 eosinophil Anatomy 0.000 description 3
- 238000007689 inspection Methods 0.000 description 3
- 210000003463 organelle Anatomy 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 239000012103 Alexa Fluor 488 Substances 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 238000004820 blood count Methods 0.000 description 2
- 210000000170 cell membrane Anatomy 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 238000000684 flow cytometry Methods 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 238000002847 impedance measurement Methods 0.000 description 2
- 230000002934 lysing effect Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000002736 nonionic surfactant Substances 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 230000035484 reaction time Effects 0.000 description 2
- 238000011895 specific detection Methods 0.000 description 2
- JDRSMPFHFNXQRB-CMTNHCDUSA-N Decyl beta-D-threo-hexopyranoside Chemical compound CCCCCCCCCCO[C@@H]1O[C@H](CO)C(O)[C@H](O)C1O JDRSMPFHFNXQRB-CMTNHCDUSA-N 0.000 description 1
- XXACTDWGHQXLGW-UHFFFAOYSA-M Janus Green B chloride Chemical compound [Cl-].C12=CC(N(CC)CC)=CC=C2N=C2C=CC(\N=N\C=3C=CC(=CC=3)N(C)C)=CC2=[N+]1C1=CC=CC=C1 XXACTDWGHQXLGW-UHFFFAOYSA-M 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 229910052783 alkali metal Inorganic materials 0.000 description 1
- 208000014759 blood platelet disease Diseases 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 239000003093 cationic surfactant Substances 0.000 description 1
- 230000006037 cell lysis Effects 0.000 description 1
- 239000002738 chelating agent Substances 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 230000008094 contradictory effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 229940073499 decyl glucoside Drugs 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 238000005538 encapsulation Methods 0.000 description 1
- 150000002338 glycosides Chemical class 0.000 description 1
- 150000003278 haem Chemical class 0.000 description 1
- 230000002949 hemolytic effect Effects 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- IKEOZQLIVHGQLJ-UHFFFAOYSA-M mitoTracker Red Chemical compound [Cl-].C1=CC(CCl)=CC=C1C(C1=CC=2CCCN3CCCC(C=23)=C1O1)=C2C1=C(CCC1)C3=[N+]1CCCC3=C2 IKEOZQLIVHGQLJ-UHFFFAOYSA-M 0.000 description 1
- 210000003470 mitochondria Anatomy 0.000 description 1
- 150000002772 monosaccharides Chemical group 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 125000001453 quaternary ammonium group Chemical class 0.000 description 1
- MYFATKRONKHHQL-UHFFFAOYSA-N rhodamine 123 Chemical compound [Cl-].COC(=O)C1=CC=CC=C1C1=C2C=CC(=[NH2+])C=C2OC2=CC(N)=CC=C21 MYFATKRONKHHQL-UHFFFAOYSA-N 0.000 description 1
- 238000007493 shaping process Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 229930002600 steroidal saponin Natural products 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 125000000542 sulfonic acid group Chemical group 0.000 description 1
- 239000012085 test solution Substances 0.000 description 1
- ANRHNWWPFJCPAZ-UHFFFAOYSA-M thionine Chemical compound [Cl-].C1=CC(N)=CC2=[S+]C3=CC(N)=CC=C3N=C21 ANRHNWWPFJCPAZ-UHFFFAOYSA-M 0.000 description 1
- CEYYIKYYFSTQRU-UHFFFAOYSA-M trimethyl(tetradecyl)azanium;chloride Chemical compound [Cl-].CCCCCCCCCCCCCC[N+](C)(C)C CEYYIKYYFSTQRU-UHFFFAOYSA-M 0.000 description 1
- 150000008130 triterpenoid saponins Chemical class 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/1031—Investigating individual particles by measuring electrical or magnetic effects
- G01N15/12—Investigating individual particles by measuring electrical or magnetic effects by observing changes in resistance or impedance across apertures when traversed by individual particles, e.g. by using the Coulter principle
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
- G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
- G01N2015/018—Platelets
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1019—Associating Coulter-counter and optical flow cytometer [OFC]
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/49—Blood
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Dispersion Chemistry (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
一种血液检测方法和相应的血液分析系统,该方法包括:用含有溶血剂的第一试剂处理血液样本以获得待测试样(S11),其中溶血剂将血液样本中的红细胞裂解为碎片;通过光学检测装置(50,200)获得待测试样的光学信息(S12),其中使待测试样中的粒子逐个通过光学检测装置(50,200)的检测区并且利用光学检测装置(50,200)的光源(101)对待测试样中的粒子进行照射,以获取待测试样的光学信息,其中光源(101)配置为发射波长小于488nm的光;和根据光学信息中的至少两种获得血小板的光学信息(S13)和/或网织红细胞的光学信息,其中在裂解血液样本中的红细胞的检测通道中获取血小板和/或网织红细胞的光学信息。
Description
PCT国内申请,说明书已公开。
Claims (36)
- PCT国内申请,权利要求书已公开。
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/CN2019/104451 WO2021042307A1 (zh) | 2019-09-04 | 2019-09-04 | 血液检测方法及血液分析系统 |
Publications (1)
Publication Number | Publication Date |
---|---|
CN114270167A true CN114270167A (zh) | 2022-04-01 |
Family
ID=74852676
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201980099668.7A Pending CN114270167A (zh) | 2019-09-04 | 2019-09-04 | 血液检测方法及血液分析系统 |
Country Status (2)
Country | Link |
---|---|
CN (1) | CN114270167A (zh) |
WO (1) | WO2021042307A1 (zh) |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2272624A1 (en) * | 1996-11-20 | 1998-05-28 | Biochem Immunosystems Inc. | Hemoglobin measurement device |
SE531041C2 (sv) * | 2006-07-17 | 2008-11-25 | Hemocue Ab | Räkning av trombocyter |
CN102331411A (zh) * | 2011-07-08 | 2012-01-25 | 无锡荣兴科技有限公司 | 一种具有蓝色半导体激光器的血液细胞分析仪 |
CN104458541A (zh) * | 2013-09-12 | 2015-03-25 | 深圳迈瑞生物医疗电子股份有限公司 | 红细胞血红蛋白含量的分析方法、装置及血液细胞分析仪 |
JP6710512B2 (ja) * | 2015-09-14 | 2020-06-17 | シスメックス株式会社 | 血液分析装置、血液分析方法及びコンピュータプログラム |
WO2018217923A1 (en) * | 2017-05-25 | 2018-11-29 | Abbott Laboratories | Methods and systems for sample analysis |
-
2019
- 2019-09-04 WO PCT/CN2019/104451 patent/WO2021042307A1/zh active Application Filing
- 2019-09-04 CN CN201980099668.7A patent/CN114270167A/zh active Pending
Also Published As
Publication number | Publication date |
---|---|
WO2021042307A1 (zh) | 2021-03-11 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US11193875B2 (en) | Method for discriminating red blood cells from white blood cells by using forward scattering from a laser in an automated hematology analyzer | |
EP1250585B1 (en) | Method and apparatus for analyzing cells in a whole blood sample | |
CA2207396C (en) | Method for rapid and simultaneous analysis of nucleated red blood cells | |
US5874310A (en) | Method for differentiation of nucleated red blood cells | |
US5917584A (en) | Method for differentiation of nucleated red blood cells | |
EP2694960B1 (en) | Identifying and enumerating early granulated cells (egcs) | |
US9797824B2 (en) | Method for hematology analysis | |
CN111602052B (zh) | 一种血液检测方法及血液分析系统 | |
US20080131898A1 (en) | Method for measuring biological sample and measuring apparatus therefor | |
US20200150021A1 (en) | Bone marrow fluid analysis method, sample analyzer, and non-transitory storage medium | |
JP3822625B2 (ja) | 血液学的コントロール製品 | |
US5840515A (en) | Erythrocyte lysis reagent, and its use in methods for isolating and differentiating leucocytes | |
US20230296591A1 (en) | Sample analysis method, sample analyzer, and computer-readable storage medium | |
JP4279900B2 (ja) | 細胞生存率、有核赤血球、および白血球分類の同時分析方法 | |
CN114270167A (zh) | 血液检测方法及血液分析系统 | |
US10215683B2 (en) | Light scatter based apparatus and methods for hematology analysis using only three detectors | |
Jeffery | Flow Cytometry | |
CN117907197A (zh) | 血液细胞分析仪和血液细胞分析方法 | |
CN114252633A (zh) | 样本分析系统、样本检测方法、组合物及其应用 | |
CN118258993A (zh) | 用于血液分析的试剂、血液分析方法及血液分析系统 | |
JP2012103267A (ja) | 血液学的試料の測定装置 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination |