Background
Trifolium pratense (Trifolium Linn), also called clover, Trifolium pratense. A perennial herb of the genus Trifolium of the family Leguminosae; the native Europe and the North Africa are cultivated all over the world, the number of the common wild species and the introduced species in China is 8, and the more common species for cultivation are red clover, white clover and the like. Axletree grass is an important pasture grass crop with wide cultivation significance, and is also an important green manure crop, garden landscape plants and water and soil conservation plants. The main roots of the axletree grass are thin and short, the lateral roots and fibrous roots are developed, most of the roots are distributed within 20cm of the surface layer, and nodules have good nitrogen fixation effect and have small contradiction between water and fertilizer competition with fruit trees; the axletree grass flowers and leaves have higher ornamental value, the green period is long, the flowering period is long, the axletree grass flowers and leaves are resistant to trampling, the shade is resistant, the compact lawn with the thickness of 20-30cm and high coverage rate can be formed, the growth of various weeds can be effectively inhibited, and the axletree grass flowers and leaves can be used as a green land closed lawn for road pavement, ditch edge and embankment slope protection and soil conservation or parks, districts, units, schools and the like. The whole herba Trifolii Pratentis for medicinal use has effects of clearing heat, cooling blood, relieving cough, relieving asthma, and relieving spasm; herba Trifolii Pratentis contains flavonoids, proteins, amino acids, saccharides and vitamins, and its extract can be used in health food.
The axyrium vulgare has wide application prospect as green manure crops, landscape plants and water and soil conservation plants, and also has certain application at home and abroad. At present, the actual sowing emergence rate of the axletree grass is 65-80%, the fresh rate exceeds 90%, and the following problems exist in the aspect of sowing: 1. the seeding is greatly influenced by environmental conditions and climatic conditions, the seeding is mostly concentrated in spring and autumn, and the seeding germination rate is lower in summer and winter; 2. the axacum grass grows slowly in the seedling stage, and the grassland is easy to be invaded by weeds. In practical application, practical sowing technologies which are not limited by sowing seasons, are less influenced by environment and climate, improve seed germination rate, shorten seedling emergence time, shorten seedling period, are simple and convenient to operate, relatively simple to manage and high in popularization and application value are urgently needed.
The information disclosed in this background section is only for enhancement of understanding of the general background of the invention and should not be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person skilled in the art.
Disclosure of Invention
The invention aims to provide a method for improving the emergence rate of axle grass in sowing, so as to overcome the defect of low emergence rate of axle grass.
In order to achieve the aim, the invention provides a method for improving the seedling emergence rate of axletree grass, which comprises the following steps:
(1) seed treatment: taking and cleaning seeds of the Trifolium Pratense L, and then sterilizing;
(2) preparing a seed dressing matrix: mixing the medicament and the matrix according to the volume ratio of 1: 3-8, mixing uniformly;
(3) uniformly mixing the axicon seeds and the seed-dressing matrix according to the volume ratio of 1:1-3, then placing the stirred seeds and the seed-dressing matrix into a container, keeping the humidity of the matrix in the container at 20-35%, and standing for 10-30h at the temperature of 25-28 ℃;
(4) sowing: wetting a sowing ground with water before sowing, uniformly scattering seeds mixed with the seed mixing matrix on the sowing ground, and spraying water on the sowing ground after sowing;
(5) and (5) management after sowing.
Preferably, in the above technical scheme, the seeds in step (1) comprise red clover or/and white clover.
Preferably, in the above technical scheme, the step (1) of sterilizing comprises soaking the seeds in a potassium permanganate solution with a concentration of 800-1200mg/L for 3-15 min.
Preferably, in the above technical scheme, the medicament in step (2) comprises: one or more of gibberellin solution, 6-benzylaminopurine solution, indole-3-butyric acid solution, naphthylacetic acid solution, ascorbic acid solution and pyroligneous solution.
Preferably, in the above technical scheme, the medicament in step (2) comprises: 500-700mg/L gibberellin solution, 100-300 mg/L6-benzylaminopurine solution, 200-400mg/L indole-3-butyric acid solution, 300-500mg/L naphthylacetic acid solution, 500-800mg/L ascorbic acid solution and 200-350 times wood vinegar solution; the volume ratio of mixing is: 1-3:1-3: 1-3:1-3: 1-3: 1-3.
Preferably, in the above technical scheme, the matrix in step (2) is mainly prepared from the following raw materials: peat soil, coconut husk, carbonized rice hull powder, loess and plant ash; the volume ratio of the peat soil, the coconut coir, the carbonized rice hull powder, the loess and the plant ash is 1-5:1-5:1-3:1-3: 1-5.
Preferably, in the above technical scheme, the matrix in step (2) is mainly prepared from the following raw materials: peat soil, coconut husk, carbonized rice hull powder, loess and plant ash; the volume ratio of the peat soil to the coconut coir to the carbonized rice hull powder to the loess to the plant ash is 2:2:1:1: 2.
Preferably, in the above technical scheme, when the matrix is prepared, the preparation raw materials are firstly screened by a mesh screen with the mesh size of 1.10-1.70mm and the standard mesh number of 10-16 meshes, and then are uniformly mixed.
Preferably, in the above technical solution, the management after the sowing in step (5) is that water is sprayed on the sowing land every 1-2 days after the sowing.
Compared with the prior art, the invention has the following beneficial effects:
(1) the method for improving the seedling emergence rate of the axyridis grass in the invention has the advantages of rapid seed germination, large root differentiation amount, more than 95% of seed seedling emergence rate, 15% higher seeding rate compared with the method without the seeds, and 1.5kg reduction of the seed consumption per mu compared with the method without the seeds with the 5.5kg per mu. The germination rate and the seedling emergence survival rate of the axyridis semen can be improved, and the seed consumption is saved.
(2) The germination time is accelerated, the seeds can germinate by more than 80 percent 7 days after the seeds are sowed, and the germination rate of the seeds exceeds 95 percent 15 days after the seeds are sowed.
(3) The seedling stage is shortened, the axletree grass grows rapidly in the seedling stage, and the damage of the axletree grass by weeds in the seedling stage is prevented.
(4) Can be sowed in four seasons, and overcomes the problem that the germination rate is low due to the influence of environmental temperature in summer and winter.
(5) The seed dressing matrix has low cost, the coconut chaff is taken as a rich natural resource in coastal areas in south China, the carbonized rice husks and the plant ash are agricultural production wastes, the recycling price is low, the carbonized rice husks and the plant ash can be regenerated, and the seed dressing matrix can be stored for more than half a year after being prepared once.
Detailed Description
The following detailed description of the present invention will be given with reference to specific examples, but it should be understood that the scope of the present invention is not limited to the specific embodiments.
Example 1
A method for improving the emergence rate of axletree grass in sowing comprises the following steps:
(1) seed treatment: taking the seed of Trifolium Pratense L.and cleaning the seed, which comprises red Trifolium Pratense L.or/and white Trifolium Pratense L., and sterilizing, wherein the seed is soaked in potassium permanganate solution with concentration of 1200mg/L for 5 min.
(2) Preparing a seed dressing matrix: mixing the medicament and the matrix according to the volume ratio of 1:3, fully and uniformly stirring and mixing by using a stirrer;
wherein, the Chinese medicine preparation in the step (2) comprises: 700mg/L gibberellin (GA3) solution, 100 mg/L6-benzylaminopurine (6-BA) solution, 200mg/L indole-3-butyric acid (IBA) solution, 300mg/L naphthylacetic acid (NAA) solution, 800mg/L ascorbic acid (VC) solution, and 200 times liquid pyroligneous solution; the volume ratio of mixing is: 1:3:1: 3:1: 1.
the substrate in the step (2) is mainly prepared from the following raw materials: peat soil, coconut husk, carbonized rice hull powder, loess and plant ash; the volume ratio of the peat soil, the coconut coir, the carbonized rice hull powder, the loess and the plant ash is 5:1:3:1: 1; when in preparation, the raw materials are screened by a mesh screen with the mesh size of 1.65mm and the standard mesh number of 10 meshes, and then are mixed uniformly.
(3) Uniformly mixing the axicon seeds and the seed-dressing matrix according to the volume ratio of 1:2, then placing the stirred seeds and the seed-dressing matrix into a container with an upper part not closed, keeping the humidity of the matrix in the container at 20-35%, and standing for 10h under the condition that the environment temperature of the container is 25-28 ℃;
(4) sowing: the sowing amount is 5.5 kg/mu calculated according to the weight of the clean seeds, the sowing land is wetted by water before sowing, the seeds mixed with the seed mixing matrix are uniformly scattered on the land needing sowing, and water is sprayed on the sowing land after sowing;
(5) and (4) managing after sowing, namely spraying water on the sowing land once every 1-2 days if the sowing land does not rain, and sprouting can be realized 5-7 days after sowing.
Example 2
A method for improving the emergence rate of axletree grass in sowing comprises the following steps:
(1) seed treatment: taking the seed of Trifolium Pratense L.for cleaning seeds, which comprises Trifolium Pratense L.or/and Trifolium Pratense L., and sterilizing, wherein the seeds are soaked in 1000mg/L potassium permanganate solution for 8 min.
(2) Preparing a seed dressing matrix: mixing the medicament and the matrix according to the volume ratio of 1: 4, mixing, fully and uniformly stirring and mixing by using a stirrer;
wherein, the Chinese medicine preparation in the step (2) comprises: 600mg/L gibberellin (GA3) solution, 200 mg/L6-benzylaminopurine (6-BA) solution, 300mg/L indole-3-butyric acid (IBA) solution, 400mg/L naphthylacetic acid (NAA) solution, 600mg/L ascorbic acid (VC) solution, and 250-fold liquid pyroligneous solution; the volume ratio of mixing is: 1:1: 1:1: 1: 1.
the substrate in the step (2) is mainly prepared from the following raw materials: peat soil, coconut husk, carbonized rice hull powder, loess and plant ash; the volume ratio of the mixture of peat soil, coconut husk, carbonized rice hull powder, loess and plant ash is 2:2:1:1: 2; during preparation, the raw materials are screened by a mesh screen with the mesh size of 1.53mm and the standard mesh number of 12 meshes, and then are uniformly mixed.
(3) Uniformly mixing the axicon seeds and the seed-dressing matrix according to the volume ratio of 1:1, then placing the stirred seeds and the seed-dressing matrix into a container with an upper part not closed, keeping the humidity of the matrix in the container at 20-35%, and standing for 18h under the condition that the environment temperature of the container is 25-28 ℃;
(4) sowing: the sowing amount is 5.5 kg/mu calculated according to the weight of the clean seeds, the sowing land is wetted by water before sowing, the seeds mixed with the seed mixing matrix are uniformly scattered on the land needing sowing, and water is sprayed on the sowing land after sowing;
(5) and (4) managing after sowing, namely spraying water on the sowing land once every 1-2 days if the sowing land does not rain, and sprouting can be realized 5-7 days after sowing.
Example 3
A method for improving the emergence rate of axletree grass in sowing comprises the following steps:
(1) seed treatment: taking the seed of the Trifolium Pratense L.seed, which comprises red Trifolium Pratense L.or/and white Trifolium Pratense L., and sterilizing, wherein the seed is soaked in potassium permanganate solution with concentration of 700mg/L for 15 min.
(2) Preparing a seed dressing matrix: mixing the medicament and the matrix according to the volume ratio of 1: 8, mixing, and fully stirring and uniformly mixing by using a stirrer;
wherein, the Chinese medicine preparation in the step (2) comprises: 500mg/L gibberellin (GA3) solution, 300 mg/L6-benzylaminopurine (6-BA) solution, 400mg/L indole-3-butyric acid (IBA) solution, 300mg/L naphthylacetic acid (NAA) solution, 500mg/L ascorbic acid (VC) solution, 350 times liquid pyroligneous solution; the volume ratio of mixing is: 3:1: 3:1: 3: 3.
the substrate in the step (2) is mainly prepared from the following raw materials: peat soil, coconut husk, carbonized rice hull powder, loess and plant ash; the volume ratio of the peat soil, the coconut coir, the carbonized rice hull powder, the loess and the plant ash is 1:5:1:3: 5; when in preparation, the raw materials are screened by a mesh screen with the mesh size of 1.30mm and the standard mesh number of 16 meshes, and then are mixed uniformly.
(3) Uniformly mixing the axicon seeds and the seed-dressing matrix according to the volume ratio of 1:3, then placing the stirred seeds and the seed-dressing matrix into a container with an upper part not closed, keeping the humidity of the matrix in the container at 20-35%, and standing for 25h under the condition that the environment temperature of the container is 25-28 ℃;
(4) sowing: the sowing amount is 5.5 kg/mu calculated according to the weight of the clean seeds, the sowing land is wetted by water before sowing, the seeds mixed with the seed mixing matrix are uniformly scattered on the land needing sowing, and water is sprayed on the sowing land after sowing;
(5) and (4) managing after sowing, namely spraying water on the sowing land once every 1-2 days if the sowing land does not rain, and sprouting can be realized 5-7 days after sowing.
Comparison of experiments
The method described in examples 1-3 was used in the actual sowing of Trifolium pratense, and the germination of seeds directly sown without the method was used as a Control (CK), and the survival rate, rooting rate and other indicators of the sown seeds were determined, and the results are shown in Table 1.
TABLE 1 determination and comparison of germination indexes of lower axyridis seeds by different sowing methods
As can be seen from Table 1, the samples and the control were each measured at 1g/1.25m2The sowing ratio of the method is adopted for sowing, the germination rate of the axylograss seeds and the length of the axylograss stems sowed by the method are obviously higher than those of a control in each period of measurement, the coverage rate of the axylograss seeds sowed by the method for 30 days is obviously higher than that of the control, and the difference between the germination rate and the coverage rate of the axylograss seeds sowed for 30 days in each embodiment is not obvious; the germination time of the axyridis seeds sowed by the method is obviously higher than that of the control. By combining with actual sampling observation, the growth states of the root system and the overground part of the carex axacuminata seeds after germination are better, and the length of the root system, the length of the seedling stem and the thickness of the seedling stem are obviously higher than those of a control. The application provides a new technical support for the axylon grass to improve the seeding efficiency in green manure application, landscape application and water and soil conservation application and save the seeding cost.
The foregoing descriptions of specific exemplary embodiments of the present invention have been presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain certain principles of the invention and its practical application to enable one skilled in the art to make and use various exemplary embodiments of the invention and various alternatives and modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.