CN101702981B - Method for artificially culturing Chinese medicinal plant of Chinese pulsatilla root - Google Patents

Method for artificially culturing Chinese medicinal plant of Chinese pulsatilla root Download PDF

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CN101702981B
CN101702981B CN2009101856850A CN200910185685A CN101702981B CN 101702981 B CN101702981 B CN 101702981B CN 2009101856850 A CN2009101856850 A CN 2009101856850A CN 200910185685 A CN200910185685 A CN 200910185685A CN 101702981 B CN101702981 B CN 101702981B
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chinese pulsatilla
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pulsatilla
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时维静
李立顺
王甫成
张子学
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时维静
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Abstract

The invention belongs to a culture technique of Chinese medical plants, relating to a method for artificially culturing Chinese medicinal plant of Chinese pulsatilla root. The Chinese pulsatilla root is commonly used traditional Chinese medicine, and has the functions of antibiosis, pathogen resistance, dampness elimination, insect control, body immunity enhancement, cancer resistance, anti-inflammatory, poison resistance, heat and toxic material clearing, blood cooling and diarrhea stopping, calmness, pain relieving, endotoxemia resistance and the like. Currently, Chinese medicinal plants ofChinese pulsatilla root all around the world grow widely; the artificially cultured Chinese medicinal plant of Chinese pulsatilla root in the invention comprises the techniques such as seed cold storage, gibberellin seed soaking, wet sand pregermination, seeding and field management, and Chinese medical plant harvest. The invention has the advantages that the artificial culturing technique of Chinese pulsatilla root is integral; the culturing measurement is simple, practical and reliable, the rate of germination is improved, and the saponin content of Chinese pulsatilla root is ensured, thereby improving medicinal material therapy.

Description

The method of artificial cultivation Chinese pulsatilla traditional Chinese medicine
Technical field
The invention belongs to the traditional Chinese medicine culture technique, specifically the method for the artificial cultivation traditional Chinese medicine Chinese pulsatilla.
Background technology
The traditional Chinese medicine Chinese pulsatilla is the dry root of ranunculaceae plant Chinese pulsatilla Pulsatilla chinensis (Bunge.) Regel.The Chinese pulsatilla is a kind of conventional Chinese medicine, has the function of antibiotic, disease-resistant protozoon, removing dampness and destroying parasites; Have improve immunity of organisms, suppress macrophage secretion IL-6, anticancer, anti-inflammatory, antitoxin, clearing heat and detoxicating, remove heat from the blood and relieve diarrhea, calm, analgesia, kill smart, suppress effects such as bowel movement, treating endotoxemia.Prior art is measured in per 100 gram Chinese pulsatilla medicinal materials, pulchinenoside B 4Content is high reaches 8 grams.
Current, global Chinese pulsatilla traditional Chinese medicine all is a grows wild, does not have the method and the technology of the artificial cultivation Chinese pulsatilla traditional Chinese medicine of system.Through extensively retrieving domestic and international patent document and consulting domestic and international public publication, do not see record and report that artificial cultivation Chinese pulsatilla traditional Chinese medicine is arranged.
Because the advantage that traditional Chinese medicine has stable curative effect, has no side effect, the application of traditional Chinese medicine and development are rapidly, Chinese pulsatilla medicinal material demand increases year by year, and output of the wild Chinese pulsatilla and quantity can't satisfy human needs of medical treatment.Therefore study the biology rule of the Chinese pulsatilla, the growth rhythm of research Chinese pulsatilla plant, the artificial cultivation of the method for invention artificial cultivation Chinese pulsatilla traditional Chinese medicine, the enforcement Chinese pulsatilla is very necessary.
The Chinese pulsatilla is a herbaceos perennial, plant height 15-35cm, and phyllopodium gives birth to, the thick shape of root, diameter 8-15mm.The growth of flower posterior lobe, wild flowers 1-2, prolong with the cultivation time limit, scape is counted increase can reach 10-30.The long 3-4mm of achene, villosity, the top has the featheriness place to deposit style, long 3.5-6.5cm, thousand grain weigth average out to 1.823g; Every several 100-200 grains of setting seeds.Northeast China area florescence 4-5 month, fruit phase 6-7 month; Central China area florescence 2-3 month, really April phase.The Chinese pulsatilla autumn spends bloom October, November the result, autumn flower and fruit are all luxuriant not as spring.In the Chinese pulsatilla grows wild, seed nature germination rate is extremely low, and root division is prone to mashed root, seriously influences growth and the breeding of the Chinese pulsatilla, and the output of Chinese pulsatilla traditional Chinese medicine is subjected to great restriction.
The inventor goes through the biology rule of year research Chinese pulsatilla surplus in the of ten, and the method for having invented artificial cultivation Chinese pulsatilla traditional Chinese medicine, its main points are presprouting of seeds technology, seedling growing process, seedling management and the harvesting technique etc. of the Chinese pulsatilla.The inventive method has that the Chinese pulsatilla of meeting biology rule, plant strain growth stalwartness, damage by disease and insect are few, root output height, root saponin content height, the eutherapeutic advantage of medicinal material.
Summary of the invention
The objective of the invention is to invent a kind of method that solves the artificial cultivation Chinese pulsatilla traditional Chinese medicine of a series of technical barriers such as Chinese pulsatilla seed treatment, seeding and seedling raising, field management, traditional Chinese medicine are gathered, seminal propagation.
The object of the present invention is achieved like this:
The method of artificial cultivation Chinese pulsatilla traditional Chinese medicine comprises the steps:
(1) seed treatment:
I. Chinese pulsatilla seed is refrigerated 3-6 days under 4 ℃ of-8 ℃ of conditions;
Ii. compound concentration is 20-100mgL -1Gibberellins solution, solvent is a water;
Iii. the Chinese pulsatilla seed after the above-mentioned refrigeration is inserted in the 15-25 ℃ of described Gibberellins solution and soaked seed 24 hours;
Iv. the seed that takes out after soaking seed is put into wet sand, and seed and wet sand are mixed thoroughly, covers the wet husky and 1cm straw of 2cm then respectively, and temperature 15-25 ℃, time 15-30 days;
(2) choosing ground and whole ground:
I. select high-land, deep, the well-drained sandy loam of soil layer field piece;
Ii. smash the soil of raking, play furrow wholely, furrow are wide 1 meter;
(3) seeding and seedling raising:
I. sowing time: mean temperature of air 15-25 ℃ spring or autumn;
Ii. get the seed that step (1) iv handles, rudiment can be sowed;
Iii. seeding quantity: 1 kilogram every mu;
Iv. covering fine sand and straw sheltered from heat or light, keep moisture after seed was broadcast;
V. keep ground moistening in after planting 35-50 days;
After the seedling of vi.60% grows 1-2 sheet true leaf, watered 1 time in 3-5 days;
(4) field management:
I. after the Chinese pulsatilla emerges, uprooting weed in seedling stage in 1 year, fertilising;
Ii. the annual florescence is in time plucked bud after reaching in 1 year;
Iii. the kind of applying fertilizer: 25% nitrogen-phosphorus-potassium compound fertilizer;
Iv. fertilization time: basal dressing before the sowing; The later annual 2-3 month, September use once respectively and topdress;
V. the quantity of applying fertilizer: 30 kilograms/mu in base manure, 15-20 kilogram/mu topdresses;
Vi. according to weather condition and soil moisture content, in time irritate draining;
(5) harvesting technique:
I. collecting time: plant strain growth the 3rd year, summer, August, fine day was gathered;
Ii. collecting method: deep-cut and plough deeply soil, get the plant root, the back of gathering in time cleans, dry;
Iii. gather back being completely cured in the ground, fibrous root ploughed under in the soil, rakes, and waters;
Iv '. behind the 3-4 that gathers for the first time, the Chinese pulsatilla plant root of gathering once more;
(6) seminal propagation:
I. seed propagation method is with above-mentioned step (1), (2), (3), (4);
Ii. seed collection method: when the withered white of style is deposited in Chinese pulsatilla fruit featheriness place, win with hand;
Iii. seed storage method: dry immediately after the results, place shady and cool dry place.
The method of described artificial cultivation Chinese pulsatilla traditional Chinese medicine, the described seed of step (1) is for keeping the new seed of gathering then that style is deposited in the featheriness place.
The method of described artificial cultivation Chinese pulsatilla traditional Chinese medicine, the described wet sand of step (1) iv is river sand, fineness 20-40 order, humidity is agglomerating for holding, and looses one's grip and promptly opens.
The method of described artificial cultivation Chinese pulsatilla traditional Chinese medicine, the described sowing of step (3) is for broadcasting sowing or drilling sowing depth 2-3 centimetre.
The described drying of step (5) ii is that fine day dries or 50 ℃ of oven for drying.
Key technology of the present invention is:
1, seed is refrigerated and gibberellin treatment, increase substantially percentage of seedgermination, make Chinese pulsatilla artificial cultivation become possibility.
Chinese pulsatilla thousand grain weigth is low, and seed grain is little, and individual plant is set seeds several many, adopts seminal propagation to help the large tracts of land artificial planting.But Chinese pulsatilla seed nature germination rate is extremely low, must carry out specially treated to Chinese pulsatilla seed, could improve germination rate, and the processing method of seed is:
1. seed be placed on refrigerator or refrigeration in 4-8 ℃ refrigeration 3-6 days;
2. the seed after will refrigerating was soaked seed 24 hours with Gibberellins solution, and gibberellin concentration is 20-100mgL -1, solvent is a water;
3. the vernalization of seed: seed after will soaking seed and fineness 20-40 purpose river sand are mixed thoroughly, the wet husky and 1cm straw maintenance moisture of upper cover 2cm; River sand humidity is held agglomerating, looses one's grip and promptly opens;
4. keep 15-30 days seed germination time, the firm rudiment of small quantities of seed can be sowed;
5. seed germination rate can be 40%-90% from extremely low the bringing up to of the germination rate under the nature by above-mentioned technical finesse.
Abovementioned technology, improve the former of percentage of seedgermination because:
1. germination rate be can improve after the gibberellin seed soaking, germinating time and process shortened.
2. temperature 15-25 ℃: the seed germination that influences too high or too low for temperature.
3. wet husky moisture content and degree of ventilation: ventilating helps increasing oxygen, promotes seed sprouting; Wet husky overdrying is crossed the wet germination rate that all makes and is reduced.
4. easily go rotten during the presprouting of seeds more than 2 years, germinate hardly, so use the new seed of gathering then.
5. keeping on the seed featheriness place deposits style and can improve germination rate.
2, choosing ground and whole ground: the cultivation Chinese pulsatilla should select high-land, deep, the well-drained sandy loam of soil layer.Will play furrow, furrow are advisable for wide 1 meter wholely, and length is decided according to landform; The high 15-20 of furrow centimetre; 20-30 centimetre of furrow furrow width.Chinese pulsatilla seed grain is tiny, and the bed of growing seedlings should be smashed leveling to soil block as far as possible, in order to seed germination and growth of seedling.
3, seeding and seedling raising:
Sowing time was advisable for mean temperature of air 15-25 ℃ with spring or autumn, and the firm rudiment of small quantities of seed can be sowed.Broadcast sowing, drilling all can; Because it is seed is tiny, sowing depth 2-3 centimetre, unsuitable dark.Broadcast the back and cover fine sand on the seed, again coated with the thin layer straw to shelter from heat or light and to keep moisture.
To keep ground moistening in after planting 35-50 days, in order to seed sprouting.After treating that most of seedling 1-2 sheet true leaf grows, drought-resistance ability strengthens, and waters in 3-5 days to get final product for 1 time.
4, field management:
After the Chinese pulsatilla emerges, note uprooting weed, fertilising seedling stage; Attention is in time irritated draining according to weather and soil moisture content situation.Bloom for the 1st time in the Chinese pulsatilla after planting second year October, bloom annual spring and autumn after 3 years, and in order to promote the growth of root, the florescence should in time be plucked flower bud.
5, harvesting technique:
The Chinese pulsatilla is a perennial plant, and growth 3-4 can gather.The Chinese pulsatilla has hibernation and aestivation characteristic, and to select fine day to gather August estivation period, saponin content is the highest.Plough deeply when gathering and deep-cut soil, the root of the results Chinese pulsatilla.Receive timely the cleaning in back and dry or put into 50 ℃ of oven dry of baking oven, prevent the saponin(e enzymolysis.Gather back being completely cured in the ground, fibrous root placed into the soil, and rakes, and waters; Chinese pulsatilla regeneration capacity is strong, can grow dense Chinese pulsatilla seedling on the 2nd year, and 3-4 can gather again, needn't sow again.
The present invention has invented Chinese pulsatilla seed treatment, vernalization and seedling management technology on the basis of having grasped Chinese pulsatilla biological property, make germination rate bring up to 60-90%, has solved Chinese pulsatilla seminal propagation problem, satisfies the artificial cultivation needs; Invented to guaranteeing the harvesting technique of Chinese pulsatilla drug effect: i.e. growth year 3-4, August in month of gathering; In time dry after gathering or 50 ℃ of oven dry, prevent the saponin(e enzymolysis, improve the medicinal material drug effect; Invented be completely cured to after gathering, fibrous root turn over imbed soil processing method, the growth that helps Chinese pulsatilla regrowth waits a whole set of complete skill.
The present invention has proposed Chinese pulsatilla seminal propagation, high yield technique in the world first and has guaranteed the harvesting technique of drug effect.Invented methods such as seed refrigeration, gibberellin seed soaking, wet husky seed dressing vernalization, made percentage of seedgermination bring up to 60-90%.Invented the aestivation of the guaranteeing Chinese pulsatilla drug effect in time dry method in back of gathering, gather; Invented the regrowth growing technology that to sow after the Chinese pulsatilla is excavated.Can effectively, clocklike carry out the operation of Chinese pulsatilla artificial cultivation according to the inventive method, the output and the quality of world's Chinese pulsatilla traditional Chinese medicine have been improved greatly, satisfy the needs of traditional Chinese medicine development and human treatment's disease, had significant novelty, creativeness and practicality.
Advantage of the present invention is:
1, Chinese pulsatilla artificial cultivation technique is complete, satisfies the tame needs of the Chinese pulsatilla.
2, cultivation step is easy, practical, reliable.
3, improve percentage of seedgermination.
4, guarantee the saponin content of Chinese pulsatilla root, improve the traditional Chinese medicine curative effect.
Embodiment
The present invention will be further described below by embodiment.
Embodiment 1:
The Anhui yangtse-huaihe region, artificial cultivation Chinese pulsatilla traditional Chinese medicine.1 mu of sandy loam area, 30 kilograms of basal dressings are ploughed deeply, and do furrow wholely.Furrow are wide 1 meter, long 20 meters of furrow, and furrow are high 0.2 meter, 0.25 meter of furrow furrow width.Get April in spring and have 1 kilogram in the seed that style is deposited in the featheriness place, put under 6 ℃ of conditions of refrigerator refrigeration 3 days; Putting into concentration is 60mgL -1Gibberellins solution in the seed soaking 24 hours; Taking-up is admixed seed in the moist river sand, river sand fineness 20 orders, and humidity is agglomerating for holding, and looses one's grip and promptly opens.Seed and river sand are mixed thoroughly, cover the wet husky and 1cm straw of 2cm then respectively.25 ℃ of vernalization of temperature, 18 days time; Small quantities of seed begins rudiment.The seed of rudiment is evenly spread on the furrow, cover the wet husky and 1cm straw of 1cm; Kept ground moistening in 50 days, Tu Ganze waters.After treating that 60% seedling grows the 2nd true leaf, watered 1 time in 3-5 days.After the Chinese pulsatilla emerges, note the uprooting weed in seedling stage.Fertilizer applied in the spring season in 1 year; Execute 20 kilograms/mu of 25% nitrogen-phosphorus-potassium compound fertilizers; With broadcast application between crop furrow face or crop plant.Start from 1 year autumn and in time pluck bud flowering stage.The 3rd year August in summer, fine day was gathered, and deep-cut and ploughed deeply soil, got Chinese pulsatilla plant root, and the back of gathering is in time cleaned fine day and dried; The warehouse is sent in the paper bag packing cartonning; Receive 280 kilograms of Chinese pulsatilla traditional Chinese medicines.Testing result saponin(e B4 content 7.8%.Gather back being completely cured in the ground, fibrous root ploughed under in the soil, rakes, and waters; Can grow dense Chinese pulsatilla seedling on the 2nd year, 3-4 can gather again once more.
Embodiment 2:
The area, Northeast China, artificial cultivation Chinese pulsatilla traditional Chinese medicine, 15 mu of sandy loam areas.Do furrow, furrow are wide 1 meter wholely, long 120 meters of furrow, and furrow are high 0.15 meter, 0.3 meter of furrow furrow width.Get the 5-6 month in spring and have 17 kilograms in the seed that style is deposited in the featheriness place, put under 6 ℃ of conditions of refrigerator refrigeration 3 days; Putting into concentration is 80mgL -1Gibberellins solution in the seed soaking 24 hours; Taking-up is put into moist river sand with seed, river sand fineness 40 orders, and humidity is agglomerating for holding, and looses one's grip and promptly opens.Seed and river sand are mixed thoroughly, cover the wet husky and 1cm straw of 1cm then respectively, 25 ℃ of vernalization of temperature, 20 days time; Small quantities of seed begins rudiment.The seed of rudiment evenly is sprinkling upon on the furrow, covers the wet husky and 1cm straw of 1cm; Soil is done and is then watered in 50 days, keeps ground moistening.After treating that 60% seedling grows the 2nd true leaf, watered 1 time in 3-5 days.After the Chinese pulsatilla emerges, note the uprooting weed in seedling stage.Execute 15 kilograms/mu of 25% nitrogen-phosphorus-potassium compound fertilizers 1 year spring; Execute 20 kilograms/mu of nitrogen-phosphorus-potassium compound fertilizers autumn; With broadcast application between crop furrow face or crop plant.In time pluck bud 1 year flowering stage.The 3rd year August in summer, fine day was gathered, and deep-cut and ploughed deeply soil, got Chinese pulsatilla plant root, and the back of gathering is in time cleaned fine day and dried; The warehouse is sent in the paper bag packing cartonning; Receive 260 kilograms of the Chinese pulsatillas for average every mu.Testing result pulchinenoside B 4Content 7.2%.Gather back being completely cured in the ground, fibrous root ploughed under in the soil, rakes, and waters; Can grow dense Chinese pulsatilla seedling on the 2nd year, 3-4 can gather again once more.
Embodiment 3:
Chinese pulsatilla seminal propagation:
Area, Chuzhou, Anhui, artificial propagation Chinese pulsatilla seed, 1.5 mu of sandy loam areas.40 kilograms of basal dressings are ploughed deeply and are raked, and do furrow wholely, and furrow are wide 1 meter, long 20 meters of furrow, and furrow are high 0.2 meter, 0.3 meter of furrow furrow width.Get September in autumn and have 1.5 kilograms in the seed that style is deposited in the featheriness place, put under 5 ℃ of conditions of refrigerator refrigeration 4 days; Putting into concentration is 80mgL -1Gibberellins solution in the seed soaking 24 hours; Taking-up is put into moist river sand with seed, river sand fineness 40 orders, and humidity is agglomerating for holding, and looses one's grip and promptly opens.Seed and river sand are mixed thoroughly, cover the wet husky and 1cm straw of 2cm then respectively, 25 ℃ of vernalization of temperature, 25 days time; The part seed begins rudiment.The seed of rudiment evenly is sprinkling upon on the furrow, covers the wet husky and 1cm straw of 1cm; Soil is done and is then watered in 50 days, keeps ground moistening.After treating that 60% seedling grows the 2nd true leaf, watered 1 time in 3-5 days.After the Chinese pulsatilla emerges, note the uprooting weed in seedling stage.1 year 2-3 month executed 30 kilograms of 25% nitrogen-phosphorus-potassium compound fertilizers; Apply fertilizer 30 kilograms September; 1 year fall flowering phase in time plucked bud, and in the 3rd year April in spring, the back of blooming was a seed maturity in 20-40 days, deposited style together with the featheriness place and plucked results, and mu is received 1.5 kilograms in seed.Gather the 4th year spring 2 kilograms in seed.The fine day Chinese pulsatilla traditional Chinese medicine of gathering deep-cut and ploughed deeply soil August in summer, got Chinese pulsatilla plant root, and the back of gathering is in time cleaned fine day and dried; The warehouse is sent in the paper bag packing cartonning; Receive 300 kilograms of Chinese pulsatilla traditional Chinese medicines for average every mu.Testing result saponin(e B 4Content 7.6%.Gather back being completely cured in the ground, fibrous root ploughed under in the soil, rakes, and waters; Next year can grow dense Chinese pulsatilla seedling, 3-4, can gather once more again seed and Chinese pulsatilla traditional Chinese medicine.
Be the preferred embodiments of the present invention only below, be not limited to the present invention.For a person skilled in the art, the present invention can have various changes and variation.Within the spirit and principles in the present invention all, any modification of being done, be equal to replacement, improvement etc., all should be included within protection scope of the present invention.

Claims (5)

1. the method for an artificial cultivation Chinese pulsatilla traditional Chinese medicine comprises the steps:
(1) seed treatment:
I. Chinese pulsatilla seed is refrigerated 3-6 days under 4 ℃ of-8 ℃ of conditions;
Ii. compound concentration is 20-100mgL -1Gibberellins solution, solvent is a water;
Iii. the Chinese pulsatilla seed after the above-mentioned refrigeration is inserted in the 15-25 ℃ of described Gibberellins solution and soaked seed 24 hours;
Iv. the seed that takes out after soaking seed is put into wet sand, and seed and wet sand are mixed thoroughly, covers the wet husky and 1cm straw of 2cm then respectively, and temperature 15-25 ℃, time 15-30 days;
(2) choosing ground and whole ground:
I. select high-land, deep, the well-drained sand loam of soil layer field piece;
Ii. smash the soil of raking, play furrow wholely, furrow are wide 1 meter;
(3) seeding and seedling raising:
I. sowing time: mean temperature of air 15-25 ℃ spring or autumn;
Ii. get the seed that step (1) iv handles, rudiment can be sowed;
Iii. seeding quantity: 1 kilogram every mu;
Iv. covering fine sand and straw sheltered from heat or light, keep moisture after seed was broadcast;
V. keep ground moistening in after planting 35-50 days;
After the seedling of vi.60% grows 1-2 sheet true leaf, watered 1 time in 3-5 days;
(4) field management:
I. after the Chinese pulsatilla emerges, uprooting weed in seedling stage in 1 year, fertilising;
Ii. the annual florescence is in time plucked bud after reaching in 1 year;
Iii. the kind of applying fertilizer: 25% nitrogen-phosphorus-potassium compound fertilizer;
Iv. fertilization time: basal dressing before the sowing; The later annual 2-3 month, September use once respectively and topdress;
V. the quantity of applying fertilizer: 30 kilograms/mu in base manure, 15-20 kilogram/mu topdresses;
Vi. according to weather condition and soil moisture content, in time irritate draining;
(5) harvesting technique:
I. collecting time: plant strain growth the 3rd year, summer, August, fine day was gathered;
Ii. collecting method: deep-cut and plough deeply soil, get the plant root, the back of gathering in time cleans, dry;
Iii. gather back being completely cured in the ground, fibrous root ploughed under in the soil, rakes, and waters;
Iv. gather for the first time behind the 3-4 Chinese pulsatilla plant root of gathering once more;
(6) seminal propagation:
I. seed propagation method is with above-mentioned step (1), (2), (3), (4);
Ii. seed collection method: when the withered white of style is deposited in Chinese pulsatilla fruit featheriness place, win with hand;
Iii. seed storage method: dry immediately after the results, place shady and cool dry place.
2. the method for artificial cultivation Chinese pulsatilla traditional Chinese medicine according to claim 1 is characterized in that: the described seed of step (1) is for keeping the new seed of gathering then that style is deposited in the featheriness place.
3. the method for artificial cultivation Chinese pulsatilla traditional Chinese medicine according to claim 1 is characterized in that: the described wet sand of step (1) iv is river sand, fineness 20-40 order, and humidity is agglomerating for holding, and looses one's grip and promptly opens.
4. the method for artificial cultivation Chinese pulsatilla traditional Chinese medicine according to claim 1 is characterized in that: the described sowing of step (3) is for broadcasting sowing or drilling sowing depth 2-3 centimetre.
5. the method for artificial cultivation Chinese pulsatilla traditional Chinese medicine according to claim 1 is characterized in that: the described drying of step (5) ii is that fine day dries or 50 ℃ of oven for drying.
CN2009101856850A 2009-11-30 2009-11-30 Method for artificially culturing Chinese medicinal plant of Chinese pulsatilla root Expired - Fee Related CN101702981B (en)

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Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103039157B (en) * 2013-01-18 2014-07-09 通化师范学院 Rapid after-ripening and germination method of pulsatilla koreana seeds
CN104365211B (en) * 2014-09-12 2016-06-29 陈小洁 One breaks the seed dormant method of the Radix Pulsatillae
CN104542280A (en) * 2014-12-24 2015-04-29 临沂大学 Pulsatilla tongkangensis somatic embryogenesis and plant regeneration method
CN104782367A (en) * 2015-04-15 2015-07-22 万邦德(湖南)天然药物有限公司 Artificial cultivation method for savatier monochasma herb
CN107455106A (en) * 2016-06-03 2017-12-12 聂后昌 Soil plantation is not treaded on
CN106818178A (en) * 2017-02-09 2017-06-13 合肥隆扬农业科技有限公司 The implantation methods of the Chinese bulbul
CN111247972A (en) * 2020-03-12 2020-06-09 惠州市九惠制药股份有限公司 Harvesting and storing method of savatier salmoides seeds

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
左桂芬等.白头翁的栽培.《特种经济动植物》.2002,(第6期),第25页. *
时维静等.凤阳县野生白头翁生物学特性的研究.《安徽技术师范学院学报》.2005,第19卷(第1期),第19-20页. *
时维静等.野生白头翁一年中皂苷的含量变化.《中国林副特产》.2006,(第4期),第19-20页. *

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