CN110140051A - 网织红细胞模拟粒子、血小板模拟粒子制备方法及质控物 - Google Patents
网织红细胞模拟粒子、血小板模拟粒子制备方法及质控物 Download PDFInfo
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- CN110140051A CN110140051A CN201780081863.8A CN201780081863A CN110140051A CN 110140051 A CN110140051 A CN 110140051A CN 201780081863 A CN201780081863 A CN 201780081863A CN 110140051 A CN110140051 A CN 110140051A
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Abstract
一种网织红细胞模拟粒子、血小板模拟粒子制备方法及质控物。网织红细胞模拟粒子制备方法，采用N‑羟基琥珀酰亚胺活化的蛋白荧光染料，对体积60‑120fL的哺乳动物无核红细胞染色，并对无核红细胞固定处理，制成网织红细胞模拟粒子。血小板模拟粒子制备采用体积2‑25fL的哺乳动物无核红细胞，其余与网织红细胞模拟粒子相同。制备方法包括：采用N‑羟基琥珀酰亚胺活化的蛋白荧光染料对不同体积的哺乳动物无核红细胞染色，分别获得网织红细胞模拟粒子和血小板模拟粒子，其散点图荧光与体积方向接近新鲜血网织红细胞、网织血小板和血小板散点图分布；制备的模拟粒子稳定性好，不干扰其它细胞粒子的计数分类。
Description
PCT国内申请，说明书已公开。
Claims (19)
- PCT国内申请，权利要求书已公开。
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110162478.4A CN112986589B (zh) | 2017-01-05 | 2017-01-19 | 网织红细胞模拟粒子、血小板模拟粒子制备方法及质控物 |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNPCT/CN2017/070307 | 2017-01-05 | ||
| CN2017070307 | 2017-01-05 | ||
| PCT/CN2017/071755 WO2018126499A1 (zh) | 2017-01-05 | 2017-01-19 | 网织红细胞模拟粒子、血小板模拟粒子制备方法及质控物 |
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| CN202110162478.4A Division CN112986589B (zh) | 2017-01-05 | 2017-01-19 | 网织红细胞模拟粒子、血小板模拟粒子制备方法及质控物 |
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| CN110140051A true CN110140051A (zh) | 2019-08-16 |
| CN110140051B CN110140051B (zh) | 2021-03-05 |
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| CN201780081863.8A Active CN110140051B (zh) | 2017-01-05 | 2017-01-19 | 网织红细胞模拟粒子、血小板模拟粒子制备方法及质控物 |
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| CN112986589B (zh) | 2023-01-24 |
| US20190352609A1 (en) | 2019-11-21 |
| CN112986589A (zh) | 2021-06-18 |
| CN110140051B (zh) | 2021-03-05 |
| WO2018126499A1 (zh) | 2018-07-12 |
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