CN109691312A - A kind of rare tea tree single plant resource grafting plus cuttage fast breeding method - Google Patents

A kind of rare tea tree single plant resource grafting plus cuttage fast breeding method Download PDF

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CN109691312A
CN109691312A CN201910035667.8A CN201910035667A CN109691312A CN 109691312 A CN109691312 A CN 109691312A CN 201910035667 A CN201910035667 A CN 201910035667A CN 109691312 A CN109691312 A CN 109691312A
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grafting
tea
cuttage
root
scion
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CN109691312B (en
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娄艳华
何卫中
吉庆勇
疏再发
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LISHUI INSTITUTE OF AGRICULTURE SCIENCE
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • A01G2/30Grafting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • A01G2/10Vegetative propagation by means of cuttings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • A01G24/12Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
    • A01G24/15Calcined rock, e.g. perlite, vermiculite or clay aggregates
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/28Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Soil Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Cultivation Of Plants (AREA)
  • Catching Or Destruction (AREA)

Abstract

The invention belongs to tea tree multiplication technique field more particularly to a kind of rare tea tree single plant resource grafting plus cuttage fast breeding methods.A kind of rare tea tree single plant resource grafting plus cuttage fast breeding method, include the following steps: the processing of (1) brick wood;(2) processing of scion;(3) low level is grafted;(4) grafting post-processing;(5) preparation of non-woven bag as substrate;(6) clip of cuttage fringe bar;(7) cuttings is handled;(8) it is managed after cuttage;(9) tea shoot is out of the garden, and expands the radix of tea germplasm in a short time by above method, shortens and expands numerous period, and be rapidly converted into productivity, increases Tea Breeding material.Tea Germplasm radix can be expanded in a short time by the grafting of this patent and rapid propagation method, and the survival rate and growth potential of single plant tea resource reach remarkable result, this combined method meets the needs of rare tea tree single plant resource fast breeding seedling.

Description

A kind of rare tea tree single plant resource grafting plus cuttage fast breeding method
Technical field
The invention belongs to tea tree multiplication technique field more particularly to a kind of rare tea tree single plant resource grafting plus cuttage are quick Mating system.
Background technique
Tea Germplasm is the material base of Tea Breeding, genetic research and Commercial cultivation.Excellent Tea Germplasm It is rare numbers, the limited rare Tea Germplasm of distribution, is the treasured for carrying out the researchs such as tea tree genetic diversity, crossbreeding Your material.
It is rare that many elite seedling resources such as yellow, albefaction and purpleization etc. are excavated in group kind tea place and old tea place now Resources of Tea Plant, and how fast breeding is carried out to these the excellent Resources of Tea Plant having found, and put into tealeaves in a short time It is had become in production for one of breeder's urgent problem.
Tradition tea tree mating system survival rate is lower at present, and the population expansion period is longer, especially to some rare tea Single plant resource is set, the needs of excellent tea tree single plant resource fast breeding seedling is not able to satisfy, hinders tea tree elite seedling resource Excavation and utilization.
Summary of the invention
The purpose of the present invention is to provide a kind of rare excellent Resources of Tea Plant of the single plant of excavation, pass through grafting 1 from 1 plant of resource Breeding adds cuttage fast breeding method to the even more rare tea tree single plant resource grafting of 100 branches within year.
The technical solution adopted by the present invention to solve the technical problems is:
A kind of rare tea tree single plant resource grafting plus cuttage fast breeding method, include the following steps:
(1) the processing of brick wood: the Pingyang Tezaocha tree kind for being 4-6 using the age of tree is stock, with three faces before grafting starts It grinds sawtooth file and carries out sawing, the stub of height 8-12cm from the ground is formed, and the cross section of stub is smooth, in case transferring It connects;
(2) the processing of scion: using rare single plant resource tea tree branch as scion branch, scion branch is cut with cutter At the short fringe of two-sided flat wedge shape, cut the long control in face in 1.5-2cm, and two sides section is smooth, the centre of two sides section there are The bast of 0.1-0.2cm, short spike length degree 2.5-3cm, 1 healthy and strong axillary bud of band and 1 climax leaves, and ensure bevel and blade Position is not ipsilateral, in case grafting;
(3) low level is grafted: the stock straight sword solid wood handle grafting knife cross of step (1) is hit exactly into stock of vertically riving, The notch depth rived can be adjusted with 2-4cm, notch depth according to the length of scion cutting surface, remove sprouting and the flower of blade Flower bud, by step (2) in case scion be vertically bumped into stock and split interface, keep the bast of stock and scion side and forming layer equal Vertical alignment simultaneously coincide, not tiltable, and making the direction blade of scion, each blade for being bumped into notch is not interfere with each other, generally outwardly Cross-cut can be bumped into 4 scion;
(4) grafting post-processing: graft union is tied up with preservative film, then fills with water with the transparent cup of disposable plastic and places grafting The side of stub, spacing 8-12cm set up big arch shed and Small plastic shed with bamboo stick, and Small plastic shed covers above apart from ground 35-45cm The PE film of one slice width 1.2-1.8m, film bottom soil pressure tightly ensure that edge is seamless, big to encircle to prevent the evaporation of arched shed internal moisture Canopy covers the sunshade net of one layer of shading rate 80-88% away from ground 65-75cm above, and sunshade network edge is fixed with waddy, prevents from hiding Screened postive is started and is scraped off;
(5) the preparation of non-woven bag as substrate: a batch hole tray non-woven bag Light medium customized, packed matrix diameter specifications 7-9cm determines the amount of matrix according to the quantity of cuttage, and carries out disinfection to matrix and water drenching, it is ensured that every disk base material moisture is sufficient;
(6) the clip of cuttage fringe bar: clip graft 1 year after mature branch, branch bar semi-lignified is in rufous, blade at Ripe, axillary bud is intact, and without serious pest and disease damage, after when before when the morning 10 or afternoon 3, fringe bar deposits in shady place after cutting, as far as possible Accomplish that the same day cuts the same day and inserts.The short spike length degree 3cm of the cuttage of clip, 1 healthy and strong axillary bud of band and 1 climax leaves, by fringe bar nature 1 section of pitch is cut into a fringe, and pitch is too long to answer appropriate length, too short that 1 fringe should be cut into 2 sections, removes lower leaflet, female leaf mistake Greatly, it need to suitably trim;Cuttings clip is smooth, and upper and lower clip is in 45 ° and the equidirectional inclination of female leaf, and upper clip is from bastem 0.2- 0.3cm does not hurt bud when cutting;
(7) cuttings is handled: impregnating cuttings base portion, speed leaching using ABT root-inducing powder, concentration 300-500mg/L soaks 4-6s, leaching Afterwards place 4-5h again cuttage into non-woven bag;
(8) manage after cuttage: 1. water management: soil relative water content is subject in nursery lot watering, preceding nursery soil of taking root Earth relative water content 80%-90%, fine day daily water drenching 2-3 times;Nursery soil relative water content 70%-80%, fine after taking root Its water drenching 1-2 times daily can place a temperature and humidity recorder in nursery lot;2. shade management: 6-8 month temperature is high, sunlight is strong, Need lid double-layered sunshade net, obscurity 90%-95%;One layer of square of 9-10 month sunshade, obscurity is advisable with 80%-85%;November- By taking off lid alternately control light income, increased fringe bar daylighting and Leaf Photosynthetic, frost or strong sunlight weather are answered within next year 2 months Timely cover net;Before March to transplanting, sunshade net is gradually thrown off;3. nutrient management: preceding blade face of taking root needs top dressing, uses 1% tealeaves Special complex fertilizer;Take root and root growth during root it is shallow and few, Nutrient Absorption is weak, using Water soluble fertilizer pouring root;It is regarded between root system peak period Tea shoot growing way is in the ranks spreading fertilizer over the fields urea and compound fertilizer;Notice that fertilising is on a small quantity repeatedly dense after concentration is first dilute, avoids with ammonium bicarbonate and chlorination Ammonia, fertilization time should be carried out in the fine day dusk or cloudy day;4. pest management: inspecting periodically the insect pest situation of cuttage seeding, in time Worm is controlled, medicament kind, dosage and usage used are identical as production tea place;5. paying attention to periodically plucking flower bud to tea shoot topping;
(9) tea shoot is out of the garden: tea shoot is out of the garden period as 2 months-mid-March of mid-October to November or next year, should first pour before lifting It is permeable, accomplish mostly band soil, hurts root, branch, leaf less.
Preferably, hole tray non-woven bag Light medium described in step 5 is the mixed of peat, vermiculite and perlite It closes, the proportion of mixture is peat: vermiculite: perlite=2:1:1.
The advantages and benefits of the present invention are: the method for tea tree fast breeding has single node cutting, container cutting propagation to educate at present Seedling and grafting etc., practice discovery by grafting (low level grafting combines moisture film and shading net covering method) and cuttage (container without The nursery of woven fabric bag) combination mode, can by the rare excellent Resources of Tea Plant of the single plant of excavation, from 1 plant of resource by grafting 1 year within Breed it is even more to 100 branches, then by 100 root graft branches by breeding within cuttage 1 year to 1000 plants of tea shoots even It more, can be by 1 part of rare single plant tea resource fast-propagation to there is a certain number of more plants of tea trees to provide within short 2 year Source expands Tea Germplasm radix, is rapidly converted into productivity in the short time.By the grafting of this patent and quickly numerous Tea Germplasm radix can be expanded in a short time by growing method, and the survival rate of single plant tea resource and growth potential reach Remarkable result, this combined method meet the needs of rare tea tree single plant resource fast breeding seedling.
Specific embodiment
Below to a preferred embodiment of the present invention will be described in detail.
The preferred embodiment of the present invention provides a kind of rare tea tree single plant resource grafting plus cuttage fast breeding method, including presses The following steps that sequence carries out:
(1) the processing of brick wood: the Pingyang Tezaocha tree kind for being 4-6 using the age of tree is stock, with three faces before grafting starts It grinds sawtooth file and carries out sawing, the stub of height 8-12cm from the ground is formed, and the cross section of stub is smooth, in case transferring It connects;
(2) the processing of scion: using rare single plant resource tea tree branch as scion branch, scion branch is cut with cutter At the short fringe of two-sided flat wedge shape, cut the long control in face in 1.5-2cm, and two sides section is smooth, the centre of two sides section there are The bast of 0.1-0.2cm, short spike length degree 2.5-3cm, 1 healthy and strong axillary bud of band and 1 climax leaves, and ensure bevel and blade Position is not ipsilateral, in case grafting;
(3) low level is grafted: the stock straight sword solid wood handle grafting knife cross of step (1) is hit exactly into stock of vertically riving, The notch depth rived can be adjusted with 2-4cm, notch depth according to the length of scion cutting surface, remove sprouting and the flower of blade Flower bud, by step (2) in case scion be vertically bumped into stock and split interface, keep the bast of stock and scion side and forming layer equal Vertical alignment simultaneously coincide, not tiltable, and making the direction blade of scion, each blade for being bumped into notch is not interfere with each other, generally outwardly Cross-cut can be bumped into 4 scion;
(4) grafting post-processing: graft union is tied up with preservative film, then fills with water with the transparent cup of disposable plastic and places grafting The side of stub, spacing 8-12cm set up big arch shed and Small plastic shed with bamboo stick, and Small plastic shed covers above apart from ground 35-45cm The PE film of one slice width 1.2-1.8m, film bottom soil pressure tightly ensure that edge is seamless, big to encircle to prevent the evaporation of arched shed internal moisture Canopy covers the sunshade net of one layer of shading rate 80-88% away from ground 65-75cm above, and sunshade network edge is fixed with waddy, prevents from hiding Screened postive is started and is scraped off;
(5) the preparation of non-woven bag as substrate: a batch hole tray non-woven bag Light medium customized, packed matrix diameter specifications 7-9cm determines the amount of matrix according to the quantity of cuttage, and carries out disinfection to matrix and water drenching, it is ensured that every disk base material moisture is sufficient, Hole tray non-woven bag Light medium is the mixing of peat, vermiculite and perlite, and the proportion of mixture is peat: vermiculite: pearl Rock=2:1:1;
(6) the clip of cuttage fringe bar: clip graft 1 year after mature branch, branch bar semi-lignified is in rufous, blade at Ripe, axillary bud is intact, and without serious pest and disease damage, after when before when the morning 10 or afternoon 3, fringe bar deposits in shady place after cutting, as far as possible Accomplish that the same day cuts the same day and inserts.The short spike length degree 2.5-3.5cm of the cuttage of clip, 1 healthy and strong axillary bud of band and 1 climax leaves, by fringe 1 section of item nature pitch is cut into a fringe, and pitch is too long to answer appropriate length, too short that 1 fringe should be cut into 2 sections, removes lower leaflet; If female leaf is excessive, need to suitably trim;Cuttings clip is smooth, and upper and lower clip is in 45 ° and the equidirectional inclination of female leaf, and upper clip is from bud Base 0.2-0.3cm, does not hurt bud when cutting;
(7) cuttings is handled: impregnating cuttings base portion, speed leaching using ABT root-inducing powder, concentration 300-500mg/L soaks 4-6s, leaching Afterwards place 4-5h again cuttage into non-woven bag;
(8) manage after cuttage: 1. water management: soil relative water content is subject in nursery lot watering, preceding nursery soil of taking root Earth relative water content 80%-90%, fine day daily water drenching 2-3 times, rainy days depends on the circumstances;Nursery soil is opposite after taking root contains Water 70%-80%, fine day daily water drenching 1-2 times, rainy days depends on the circumstances, and a temperature and humidity record can be placed in nursery lot Instrument;2. shade management: 6-8 month temperature is high, sunlight is strong, needs lid double-layered sunshade net, obscurity 90%-95%;9-10 month sunshade net One layer of lid, obscurity is advisable with 80%-85%;By taking off lid alternately control light income, increased fringe bar daylighting within November to next year 2 months And Leaf Photosynthetic, frost or strong sunlight weather answer timely cover net;Before March to transplanting, sunshade net is gradually thrown off;3. nutrient Management: preceding blade face of taking root needs top dressing, uses 1% Special composite fertilizer for tea;Take root and root growth during root it is shallow and few, nutrient Absorb it is weak, using Water soluble fertilizer pouring root;Depending on tea shoot growing way in the ranks spreading fertilizer over the fields urea and compound fertilizer between root system peak period;Notice that fertilising will be lacked Amount is repeatedly, dense after concentration is first dilute, avoids with ammonium bicarbonate and sal-ammoniac, fertilization time should fine day at dusk or the cloudy day carries out;4. pest and disease damage Management: inspecting periodically the insect pest situation of cuttage seeding, controls worm in time, medicament kind, dosage and usage used with production tea place phase Together;5. paying attention to periodically plucking flower bud to tea shoot topping;
(9) tea shoot is out of the garden: tea shoot is out of the garden period as 2 months-mid-March of mid-October to November or next year, should first pour before lifting It is permeable, accomplish mostly band soil, hurts root, branch, leaf less.
Operating process
The processing of brick wood: the Pingyang Tezaocha tree kind for being 4-6 using the age of tree is ground before grafting starts with three faces as stock Saw gumming file carries out sawing, forms the stub of height 8-12cm from the ground, and the cross section of stub is smooth, in case grafting. The selection of brick wood is as described in Table 1 in the step (1), can be seen that stock grafting survival rate highest by grafting comparative test Be Pingyang Tezaocha tree kind, average out to 93.29%, followed by good tender tea leaves No.1 tea tree breed, average out to 84.05% is minimum Be Dragon Well tea tea tree breed, average out to 80.72%.Therefore Pingyang Tezaocha tree kind is selected then to transfer for the stock of grafting It connects and carries out sawing with three faces grinding sawtooth file before starting, form the stub of height 10cm or so from the ground, and stub is crosscutting Face is smooth, in case grafting.(being shown in Table 1)
The different single plant tea resources of table 1 graft different stock survival rate application forms
The processing of scion: using rare single plant 1-10 Resources of Tea Plant as scion branch, scion branch is cut with cutter At the short fringe of two-sided flat wedge shape, cut the long control in face in 1.5-2cm, and two sides section is smooth, the centre of two sides section there are The bast of 0.1-0.2cm, short spike length degree 2.5-3cm, 1 healthy and strong axillary bud of band and 1 climax leaves, and ensure bevel and blade Position is not ipsilateral, in case grafting.
Low level grafting: the vertical stock of riving in stock straight sword solid wood handle grafting knife cross center of step 1 is rived Notch depth with 3cm, notch depth can be adjusted according to the length of scion cutting surface, remove sprouting and the bud of blade, will Step 2 in case scion be vertically bumped into stock and split interface, keep the bast of stock and scion side and forming layer vertical right Neat and coincide, not tiltable, making the direction blade of scion, each blade for being bumped into notch is not interfere with each other outwardly.
Grafting post-processing: graft union is tied up with preservative film, then fills with water with the transparent cup of disposable plastic and places grafting tree The side of stake, spacing 10cm set up big arch shed and Small plastic shed with bamboo stick, and Small plastic shed covers a slice width apart from ground 40cm above The PE film of 1.5m, film bottom soil pressure tightly ensure that edge is seamless, and to prevent the evaporation of arched shed internal moisture, big arch shed is away from ground 70cm covers the sunshade net of one layer of shading rate 85% above, and sunshade network edge is fixed with waddy, prevents sunshade net from starting and scraping It opens.The growth potential of rare single plant 1-10 tea tree is investigated and (is shown in Table 2) after grafting half a year.
Growth potential application form after the different rare tea tree single plant resource grafting half a year of table 2
The preparation of non-woven bag as substrate: a batch hole tray non-woven bag Light medium peat customized: vermiculite: perlite=2: 1:1, packed matrix diameter specifications 8cm, the amount of matrix is determined according to the quantity of cuttage, and is carried out disinfection to matrix and water drenching, really It is sufficient to protect every disk base material moisture.
The clip of cuttage fringe bar: clip graft 1 year after mature branch, branch bar semi-lignified, be in rufous, blade at Ripe, axillary bud is intact, and without serious pest and disease damage, after when before when the morning 10 or afternoon 3, fringe bar deposits in shady place after cutting, to the greatest extent Amount accomplishes that the same day cuts the same day and inserts.Short spike length degree 3cm of the cuttage of clip or so, 1 healthy and strong axillary bud of band and 1 climax leaves.Press fringe 1 section of item nature pitch is cut into a fringe, and pitch is too long to answer appropriate length, too short that 1 fringe should be cut into 2 sections, removes lower leaflet; If female leaf is excessive, need to suitably trim;Cuttings clip is smooth, and upper and lower clip is in 45 ° and the equidirectional inclination of female leaf, and upper clip is from bud Base 0.2-0.3cm, does not hurt bud when cutting.
Cuttings processing: cuttings base portion, speed leaching are impregnated using ABT root-inducing powder, concentration 450mg/L soaks 5-7s, places after leaching 4-5h again cuttage into non-woven bag.
Manage after cuttage: 1. water management: nursery lot watering is subject to soil relative water content, nursery soil before taking root Relative water content 80%-90%, fine day daily water drenching 2-3 times, rainy days depends on the circumstances;Nursery soil relative hydration after taking root 70%-80% is measured, fine day daily water drenching 1-2 times, rainy days depends on the circumstances, and a temperature and humidity record can be placed in nursery lot Instrument.2. shade management: 6-8 month temperature is high, sunlight is strong, needs lid double-layered sunshade net, obscurity 90%-95%;9-10 month sunshade net One layer of lid, obscurity is with 80%-85%;Next year 2 months November-by taking off lid alternately control light income, increased fringe bar daylighting and leaf Piece rate of photosynthisis, frost or strong sunlight weather answer timely cover net;Before March to transplanting, sunshade net is gradually thrown off.3. nutrient pipe Reason: preceding blade face of taking root needs top dressing, uses 1% Special composite fertilizer for tea;Take root and root growth during root it is shallow and few, nutrient is inhaled Receive it is weak, using Water soluble fertilizer pouring root;Depending on tea shoot growing way in the ranks spreading fertilizer over the fields urea and compound fertilizer between root system peak period;Notice that fertilising is a small amount of Repeatedly, dense after concentration is first dilute, avoid with ammonium bicarbonate and sal-ammoniac, fertilization time should fine day at dusk or the cloudy day carries out.4. pest and disease damage pipe Reason: inspecting periodically the insect pest situation of cuttage seeding, controls worm in time, and medicament kind, dosage and usage used are identical as production tea place. 5. paying attention to periodically plucking flower bud to tea shoot topping.
Tea shoot is out of the garden: tea shoot is out of the garden period as 2 months-mid-March of November mid-October-or next year, should first irrigate before lifting Water accomplishes mostly band soil, hurts root, branch, leaf less.The 1-10 elite seedling emergence rate of cuttage can reach 95% or more, growth potential Well, and amount is more.The survival rate and growth potential of rare single plant 1-10 tea tree are investigated and (be shown in Table 3) after cuttage half a year.
Survival rate and growth potential application form after the different rare tea tree single plant resource cuttage half a year of table 3
Practice discovery: pass through grafting (low level grafting combines moisture film and shading net covering method) and cuttage (container nonwoven Cloth bag nursery) combination mode, by the rare excellent Resources of Tea Plant of the single plant of collection, from 1 plant of resource by grafting 1 year within breed It is even more to 100 branches then even more to 1000 plants of tea shoots by breeding within cuttage 1 year by 100 root graft branches, It can be short by 1 part of rare single plant tea resource fast-propagation to there is a certain number of more plants of Resources of Tea Plant within short 2 year Tea Germplasm radix is expanded in time, and the survival rate of single plant tea resource and growth potential reach remarkable result, this Kind combined method meets the needs of rare tea tree single plant resource fast breeding seedling.

Claims (2)

1. a kind of rare tea tree single plant resource grafting plus cuttage fast breeding method, it is characterised in that include the following steps:
The processing of brick wood: the Pingyang Tezaocha tree kind for being 4-6 using the age of tree is stock, with three face abrasive saws before grafting starts Tooth file carries out sawing, forms the stub of height 8-12cm from the ground, and the cross section of stub is smooth, in case grafting;
The processing of scion: using rare single plant resource tea tree branch as scion branch, scion branch is whittled into cutter two-sided The short fringe of flat wedge shape cuts the long control in face in 1.5-2cm, and two sides section is smooth, and there are 0.1-0.2cm for the centre of two sides section Bast, short spike length degree 2.5-3cm, 1 healthy and strong axillary bud of band and 1 climax leaves, and ensure that bevel is different from leaf position Side, in case grafting;
Low level grafting: the vertical stock of riving in stock straight sword solid wood handle grafting knife cross center of step (1) is rived Notch depth can be adjusted with 2-4cm, notch depth according to the length of scion cutting surface, remove sprouting and the bud of blade, will Step (2) in case scion be vertically bumped into stock and split interface, keep the bast of stock and scion side and forming layer vertical It is aligned and coincide, not tiltable, making the direction blade of scion, each blade for being bumped into notch is not interfere with each other, general cross outwardly Shape notch can be bumped into 4 scion;
Grafting post-processing: graft union is tied up with preservative film, then fills with water with the transparent cup of disposable plastic and places grafting stub Side, spacing 8-12cm set up big arch shed and Small plastic shed with bamboo stick, and Small plastic shed covers a slice width apart from ground 35-45cm above The PE film of 1.2-1.8m, film bottom soil pressure tightly ensure that edge is seamless, and to prevent the evaporation of arched shed internal moisture, big arch shed is away from ground Face 65-75cm covers the sunshade net of one layer of shading rate 80-88% above, and sunshade network edge is fixed with waddy, prevents sunshade net from lifting It rises and scrapes off;
The preparation of non-woven bag as substrate: a batch hole tray non-woven bag Light medium customized, packed matrix diameter specifications 7-9 cm, The amount of matrix is determined according to the quantity of cuttage, and is carried out disinfection to matrix and water drenching, it is ensured that every disk base material moisture is sufficient;
The clip of cuttage fringe bar: the mature branch after clip grafting 1 year, branch bar semi-lignified are in rufous, and blade is mature, armpit Bud is intact, and without serious pest and disease damage, after when before when the morning 10 or afternoon 3, fringe bar deposits in shady place after cutting, accomplish to work as far as possible It is cut the same day and inserts;
The short spike length degree 2.5-3.5cm of the cuttage of clip, 1 healthy and strong axillary bud of band and 1 climax leaves, by 1 section of fringe bar nature pitch It is cut into a fringe, pitch is too long to answer appropriate length, and too short that 1 fringe should be cut into 2 sections, removal lower leaflet, female leaf is excessive, need to fit Work as trimming;Cuttings clip is smooth, and upper and lower clip is cut in 45 ° and the equidirectional inclination of female leaf, upper clip from bastem 0.2-0.3cm When do not hurt bud;
Cuttings processing: cuttings base portion, speed leaching are impregnated using ABT root-inducing powder, concentration 300-500mg/L soaks 4-6s, places after leaching 4-5h again cuttage into non-woven bag;
Manage after cuttage: 1. water management: nursery lot watering is subject to soil relative water content, nursery soil is opposite before taking root Water content 80%-90%, fine day daily water drenching 2-3 times;Nursery soil relative water content 70%-80% after taking root, the daily water drenching of fine day 1-2 times, a temperature and humidity recorder can be placed in nursery lot;2. shade management: 6-8 month temperature is high, sunlight is strong, need to cover the double-deck screening Screened postive, obscurity 90%-95%;One layer of square of 9-10 month sunshade, obscurity is with 80%-85%;November to next year 2 months by taking off lid Alternately control light income, increases fringe bar daylighting and Leaf Photosynthetic, frost or strong sunlight weather answer timely cover net;March arrives Before transplanting, sunshade net is gradually thrown off;3. nutrient management: preceding blade face of taking root needs top dressing, uses 1% Special composite fertilizer for tea;It takes root And root is shallow and few during root growth, Nutrient Absorption is weak, using Water soluble fertilizer pouring root;It is spread depending on tea shoot growing way in the ranks between root system peak period Apply urea and compound fertilizer;Notice that fertilising is on a small quantity repeatedly dense after concentration is first dilute, avoid with ammonium bicarbonate and sal-ammoniac, fertilization time should be Fine day is at dusk or the cloudy day carries out;4. pest management: inspecting periodically the insect pest situation of cuttage seeding, control worm, medicament kind used in time Class, dosage and usage are identical as production tea place;5. paying attention to periodically plucking flower bud to tea shoot topping;
Tea shoot is out of the garden: tea shoot is out of the garden period as 2 months-mid-March of mid-October to November or next year, should first sprinkle profoundly water before lifting, Accomplish mostly band soil, hurts root, branch, leaf less.
2. a kind of rare tea tree single plant resource grafting according to claim 1 plus cuttage fast breeding method, feature exist In hole tray non-woven bag Light medium described in step 5 is the mixing of peat, vermiculite and perlite, and the proportion of mixture is Peat: vermiculite: perlite=2:1:1.
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Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110249814A (en) * 2019-05-10 2019-09-20 丽水市农业科学研究院 A kind of method of the rare special tea germplasm of fast breeding
CN110622717A (en) * 2019-11-13 2019-12-31 丽水市农业科学研究院 Landscape tea tree cultivation method
CN110679312A (en) * 2019-10-11 2020-01-14 晴隆县沙子镇果苗园艺基地 Rapid propagation method for navel orange seedlings
CN110972898A (en) * 2019-12-30 2020-04-10 江苏兴农基质科技有限公司 Matrix for tea cutting seedling test and preparation method thereof
CN111657041A (en) * 2020-06-28 2020-09-15 南京农业大学 Efficient rapid propagation method for improving tea seedling resistance
CN112056102A (en) * 2020-09-24 2020-12-11 中国农业科学院茶叶研究所 Method for promoting root growth of tea tree cutting seedlings
CN115053745A (en) * 2022-07-25 2022-09-16 丽水市农林科学研究院 Method for improving grafting survival rate of tea trees
CN117265155A (en) * 2023-08-07 2023-12-22 四川农业大学 InDel marker primer for rapidly identifying germplasm of bud She Zao tea tree and application thereof

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101611681A (en) * 2009-07-17 2009-12-30 广西壮族自治区林业科学研究院 The propagation method of using single-bud short tamarix to cut containerized seedlings
CN103202165A (en) * 2013-04-18 2013-07-17 王宗瑜 Method for cutting and raising seedlings of cliff tea
CN103262756A (en) * 2013-06-04 2013-08-28 句容市张庙茶场 Tea tree grafting seed changing method
CN103460943A (en) * 2013-09-18 2013-12-25 安顺市纳阳生态农业发展有限公司 Elite breeding method for tea-oil trees
CN103518540A (en) * 2012-07-04 2014-01-22 广西平果浪伏有机茶业有限公司 Purple tea tree cultivating method
CN106489648A (en) * 2016-10-26 2017-03-15 云南省农业科学院茶叶研究所 A kind of method of wild Dali tea fast-propagation
CN106688781A (en) * 2016-12-05 2017-05-24 东兴鑫宇实业有限公司 Grafting propagation method of yellow camellia
CN107980374A (en) * 2017-11-25 2018-05-04 广西宝树金花茶有限公司 Camellia nitidissima fast breeding method
CN109041835A (en) * 2018-06-11 2018-12-21 安徽省兰香缘农业生态发展有限责任公司 A kind of Light media nutrient bag cottage method for tealeaves

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101611681A (en) * 2009-07-17 2009-12-30 广西壮族自治区林业科学研究院 The propagation method of using single-bud short tamarix to cut containerized seedlings
CN103518540A (en) * 2012-07-04 2014-01-22 广西平果浪伏有机茶业有限公司 Purple tea tree cultivating method
CN103202165A (en) * 2013-04-18 2013-07-17 王宗瑜 Method for cutting and raising seedlings of cliff tea
CN103262756A (en) * 2013-06-04 2013-08-28 句容市张庙茶场 Tea tree grafting seed changing method
CN103460943A (en) * 2013-09-18 2013-12-25 安顺市纳阳生态农业发展有限公司 Elite breeding method for tea-oil trees
CN106489648A (en) * 2016-10-26 2017-03-15 云南省农业科学院茶叶研究所 A kind of method of wild Dali tea fast-propagation
CN106688781A (en) * 2016-12-05 2017-05-24 东兴鑫宇实业有限公司 Grafting propagation method of yellow camellia
CN107980374A (en) * 2017-11-25 2018-05-04 广西宝树金花茶有限公司 Camellia nitidissima fast breeding method
CN109041835A (en) * 2018-06-11 2018-12-21 安徽省兰香缘农业生态发展有限责任公司 A kind of Light media nutrient bag cottage method for tealeaves

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
俞惠玲等: "黄金芽低位嫁接换种技术", 《中国茶叶》 *

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110249814A (en) * 2019-05-10 2019-09-20 丽水市农业科学研究院 A kind of method of the rare special tea germplasm of fast breeding
CN110679312A (en) * 2019-10-11 2020-01-14 晴隆县沙子镇果苗园艺基地 Rapid propagation method for navel orange seedlings
CN110622717A (en) * 2019-11-13 2019-12-31 丽水市农业科学研究院 Landscape tea tree cultivation method
CN110972898A (en) * 2019-12-30 2020-04-10 江苏兴农基质科技有限公司 Matrix for tea cutting seedling test and preparation method thereof
CN111657041A (en) * 2020-06-28 2020-09-15 南京农业大学 Efficient rapid propagation method for improving tea seedling resistance
CN112056102A (en) * 2020-09-24 2020-12-11 中国农业科学院茶叶研究所 Method for promoting root growth of tea tree cutting seedlings
CN115053745A (en) * 2022-07-25 2022-09-16 丽水市农林科学研究院 Method for improving grafting survival rate of tea trees
CN115053745B (en) * 2022-07-25 2023-09-12 丽水市农林科学研究院 Method for improving grafting survival rate of tea trees
CN117265155A (en) * 2023-08-07 2023-12-22 四川农业大学 InDel marker primer for rapidly identifying germplasm of bud She Zao tea tree and application thereof

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