CN109601357B - Tiger seed seedling culture method - Google Patents
Tiger seed seedling culture method Download PDFInfo
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- CN109601357B CN109601357B CN201910071120.3A CN201910071120A CN109601357B CN 109601357 B CN109601357 B CN 109601357B CN 201910071120 A CN201910071120 A CN 201910071120A CN 109601357 B CN109601357 B CN 109601357B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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Abstract
The invention provides a black tiger seed seedling raising method, which comprises the steps of pretreating black tiger seeds by adopting a series of modes of wet sand storage, temperature change treatment and soaking by using a plant hormone solution and a potassium permanganate solution, then sowing the black tiger seeds in a plug tray which takes water moss as a matrix in a culture room or a seedling raising shed, controlling temperature, controlling humidity and shading until seedlings emerge, removing shading substances, turning to normal-temperature culture, and transplanting the black tiger seeds and the matrix to a field after 1-2 true leaves grow out from the seedlings. Compared with the existing method, the method can effectively break the dormancy of the black tiger seeds to promote germination, so that the black tiger seeds emerge about 45 days in advance, the emergence time of the black tiger seeds is shortened, the emergence rate is improved and can reach over 90 percent, and the management is convenient. The improvement of the technology has important significance for improving the quality of the black tiger afforestation nursery stock and promoting the rapid development of the black tiger industry.
Description
Technical Field
The invention relates to the technical field of plant seedling culture, in particular to a black tiger seed seedling culture method.
Background
The black tiger is a plant of Schisandra of Schisandraceae, is called as Dioscorea glauca, caulis Endadae Phaseoloidis and Dazuki, is an evergreen woody vine wild medicinal plant and wild fruit, is mainly distributed in hillsides and mountain valley miscellaneous trees of provinces (regions) such as Guangxi, Yunnan, Hunan and Sichuan, and is often wound on trees. The active components extracted from root and stem of Kadsura coccinea can be used for inhibiting vasoconstriction and increasing coronary blood flow, and has effects in resisting liver toxicity, tumor and digestive tract ulcer, resisting cancer, resisting AIDS and protecting myocardium. The black tiger fruit is unique, fragrant in smell, delicious and sweet in fruit taste, rich in vitamins and various trace elements required by human body, and the black tiger fruit is a multipurpose plant which integrates medicinal, edible, ornamental, greening and beautifying functions and has great development potential by soaking the black tiger fruit in wine.
At present, the seedlings for cultivating the black cutworms are mainly from wild or semi-wild black cutworms, the phenomena of excessive fruit taking, vine taking and root digging exist, most of the population of the species is in a decline state, the sowing and seedling raising technology is still immature, and the method is usually carried out in a mode of sowing at any time, namely, the seeds are directly sowed in open fields in a mode of drilling, broadcasting or dibbling after being picked. When the conventional sowing method is adopted, the black tiger seeds can emerge after 6 months of dormancy from sowing to emergence, and are sown in open field, the seeds and seedlings are easily damaged by rats and insects, the problems of long emergence period, difficult management, low emergence rate and the like exist, and a set of black tiger seed seedling raising method capable of effectively overcoming the defects is urgently needed.
Disclosure of Invention
The invention provides a black tiger seed seedling raising method, which solves the problems of long seedling emergence period, inconvenient field management and low seedling emergence rate of black tiger seeds.
The invention provides a black tiger seed seedling method, which comprises the following steps:
seed selection: screening seeds which are full in seeds, brown in seed coats and glossy from the collected black tiger seeds;
storing wet sand: storing the screened seeds in wet sand for 25-35 days, and turning over the seeds once every 7-10 days;
temperature change treatment: treating the seeds stored in wet sand at 1-10 deg.C for 13-15hr and at 20-30 deg.C for 9-11hr, repeating for 2-3 times;
soaking treatment: soaking the seeds subjected to temperature change treatment in a seed pretreatment solution for 20-24hr, and then soaking in a 0.1% -0.2% potassium permanganate solution for 15-30min, wherein the seed pretreatment solution is a mixed solution composed of gibberellin and naphthylacetic acid;
sowing: sowing the soaked seeds in a hole tray with a matrix of water moss in a culture room or a seedling shed, thoroughly watering the matrix with clear water, covering the sowed seeds with 0.4-0.6cm thick water moss, spraying clear water, and arranging a shade on the covered water moss;
and (3) management after sowing: controlling the temperature of the culture room or seedling shed at 20-27 deg.C and humidity of 70-90%, removing shade when seedling emergence rate reaches 25-35%, and culturing at normal temperature when seedling emergence rate reaches 65-75%;
transplanting: after 1-2 true leaves grow out from the seedling, transplanting the seedling and the matrix to a field.
After the black cutworm seeds are stored in wet sand for a certain time, the seed coat permeability of the black cutworm seeds can be improved through temperature change treatment, and the supply of oxygen and nutrients of the seeds is improved; GA3The (gibberellin) and NAA (naphthylacetic acid) are used as plant hormones, can break dormancy of black tiger seeds and promote germination of black tiger seeds, and the permeability of seed coats is improved due to previous wet sand storage and temperature change treatment, so that GA (GA) is obtained in the soaking process3The action effect of the pretreatment solution consisting of (gibberellin) and NAA (naphthylacetic acid) is further improved; the black cutworm seeds can be sterilized by soaking in the potassium permanganate solution, so that the influence of bacteria in the seedling emergence process is prevented; the sphagna as a substrate has good air permeability and moisture retention performance, is not easy to decay and can be used for a long time; after sowing, the black tiger seeds are cultured in a dark environment by covering a shade, so that the seed germination can be effectively promoted; the method has the advantages that the method can be used for sowing and culturing in a culture room or a seedling raising shed with the temperature and humidity controlled manually, the seedling emergence process of the black tiger seeds can be prevented from being influenced by wind and rain factors, the influence of mouse damage and insect damage can be avoided, in addition, under the humidity of 70-90%, the black tiger seeds can be supplemented with enough moisture, the rotten situation of the seeds can not be caused, and the smooth germination of the black tiger seeds is guaranteed.
In one embodiment of the invention, the thousand seed weight of the screened seeds is more than or equal to 220g during seed selection. The plump degree of the black cutworm seeds can influence the rate of emergence, and when the seeds are selected, the seeds with thousand seed weight more than or equal to 220g can be selected to ensure that the seeds are plump, so that certain biological activity is ensured.
In one embodiment of the invention, the weight ratio of the seeds to the wet sand is 1 (2.5-3.5) when the wet sand is stored. The mass ratio of the seeds to the wet sand is too low, so that the volume of the wet sand is too large, and the management is inconvenient; if the mass ratio is too high, the wet sand cannot effectively coat the seeds, and the seeds are easy to dry, so that the expected effect of storing the wet sand cannot be achieved. In addition, the humidity of the wet sand is determined by the degree that the wet sand is kneaded into a ball by hand and loosened by hand, the wet sand is turned and checked for 1 time in 7-10 days, and mildewed seeds are removed in time. Optionally, the weight ratio of seeds to wet sand is 1:3.
In one embodiment of the invention, the weight ratio of gibberellin to naphthylacetic acid in the seed pretreatment liquid is (3-4):1, and the total solute concentration is 40-50 mg/L. Optionally, the weight ratio of gibberellin to naphthylacetic acid in the seed pretreatment solution is 4:1, the total solute concentration is 50mg/L, and the combined plant hormone solution has the strongest physiological activity and the best promotion effect on seed germination.
In one embodiment of the invention, the plug is a 72-105-hole plastic plug, the upper opening diameter of the plastic plug is 3.2-4cm, the lower opening diameter of the plastic plug is 1.2-2cm, the plug depth is 3.5-4.5cm, and the size of the plastic plug is 54 x 28 cm. The plug tray is selected during seeding, so that mechanical operation and standard seedling management are facilitated, the plug tray can be repeatedly used after thorough cleaning and disinfection, and the plastic plug tray is low in cost, light and convenient to use in batches. The size of the plug is matched with that of the black tiger seeds, and the large size can increase the amount of used matrix and increase the seedling raising cost; too small a hole tray size can lead to poor growth of the seedling root system, root nesting and influence on the seedling quality.
In one embodiment of the invention, the shade is a newspaper, a nonwoven fabric or an opaque plastic film. The shading is beneficial to the germination of the black tiger seeds, and the shading object is selected from newspaper, non-woven fabrics or opaque plastic films, so that the black tiger seeds have the shading effect and the moisturizing effect, and the substrate and the seeds are kept at constant humidity.
The black cutworm seed seedling raising method provided by the invention comprises the steps of pretreating black cutworm seeds by adopting a series of modes of wet sand storage, temperature change treatment, seed pretreatment liquid soaking and potassium permanganate solution soaking, then sowing the black cutworm seeds in a plug tray taking water moss as a matrix in a culture room or a seedling raising shed, carrying out shading culture under the condition of manually controlling temperature and humidity until seedlings emerge, removing shading substances, carrying out normal-temperature culture, and transplanting the black cutworm seeds and the matrix to a field after the seedlings grow 1-2 true leaves. Compared with the prior art, the method for plug seedling in the greenhouse has the advantages that the dormancy of the black tiger seeds is effectively broken through the series of steps of pretreatment of the seeds and control of the germination conditions, the early germination of the black tiger seeds is promoted, the black tiger seedlings emerge about 45 days in advance, the germination time of the black tiger seeds is greatly shortened, the emergence rate is improved and can reach over 90%, and the adverse effects of rat damage, insect damage and the like can be effectively avoided. The improvement of the technology of the invention has important significance for improving the quality of the nursery stock for planting the black cutworms and promoting the rapid development of the black cutworm industry.
Detailed Description
The black tiger seed seedling raising method provided by the embodiment of the invention comprises the following steps:
seed selection: and (4) screening the seeds with full seeds, tan seed coats and luster from the collected black cutworm seeds.
And (4) screening the seeds with full seeds, tan seed coats and luster from the collected black cutworm seeds. The quality of the black cutworm seeds can influence the emergence rate of the black cutworm seeds, so that the seeds with plump seeds, tan seed coats and luster are selected when the seeds are selected, and in the embodiment of the invention, the thousand seed weight of the selected seeds is preferably more than or equal to 220g so as to ensure that the selected seeds have certain biological activity.
Storing wet sand: storing the screened seeds in wet sand for 25-35 days, and turning over the seeds once every 7-10 days.
Temperature change treatment: treating the seeds stored in wet sand at 1-10 deg.C for 13-15hr and at 20-30 deg.C for 9-11hr, respectively, and repeating for 2-3 times.
After the black cutworm seeds are stored in wet sand for a certain time, the seed coat permeability of the black cutworm seeds can be improved through temperature change treatment, the supply of oxygen and nutrients to the seeds is improved, the emergence rate is improved, and preparation is made for subsequent soaking and absorbing of the seed pretreatment liquid.
In order to ensure the storage effect of the wet sand, the weight ratio of the seeds to the wet sand is preferably 1 (2.5-3.5), and more preferably 1:3. The mass ratio of the seeds to the wet sand is too low, so that the volume of the wet sand is too large, and the management is inconvenient; if the mass ratio is too high, the wet sand cannot effectively coat the seeds, and the seeds are easy to dry, so that the expected effect of storing the wet sand cannot be achieved. In addition, the humidity of the wet sand is determined by the degree that the wet sand is kneaded into a ball by hand and loosened by hand, the wet sand is turned and checked for 1 time in 7-10 days, and mildewed seeds are removed in time.
Soaking treatment: soaking the seeds subjected to temperature change treatment in a seed pretreatment solution for 20-24hr, and then soaking in a 0.1% -0.2% potassium permanganate solution for 15-30min, wherein the seed pretreatment solution is a mixed solution composed of gibberellin and naphthylacetic acid.
GA3The (gibberellin) and NAA (naphthylacetic acid) are used as plant hormones, can break dormancy of black tiger seeds and promote germination of black tiger seeds, and the permeability of seed coats is improved due to previous wet sand storage and temperature change treatment, so that GA (GA) is obtained in the soaking process3The action effect of the pretreatment solution consisting of (gibberellin) and NAA (naphthylacetic acid) is further improved; the black cutworm seeds can be sterilized by soaking in the potassium permanganate solution, and the influence of bacteria in the seedling emergence process is prevented. In the embodiment of the invention, the weight ratio of gibberellin to naphthylacetic acid in the seed pretreatment liquid is (3-4):1, and the total solute concentration is 40-50 mg/L. More preferably, the weight ratio of the gibberellin to the naphthylacetic acid in the seed pretreatment solution is 4:1, the total solute concentration is 50mg/L, the combined plant hormone solution has the strongest physiological activity and the best promotion effect on seed germination.
Sowing: in a culture room or a seedling shed, sowing the soaked seeds in a hole tray with a matrix of water moss, thoroughly watering the matrix with clear water, covering the sown seeds with 0.4-0.6cm thick water moss, spraying clear water, and arranging a shade on the covered water moss.
The water moss as matrix has good air permeability and moisture retention, is not easy to decay, and can be used for a long time. The seeds can be moisturized by covering the water moss after sowing, an environment with constant humidity is provided, and seedling emergence of the seeds is facilitated. The plastic plug tray can be selected as the plug tray, so that the mechanized operation and the standard seedling management are facilitated, the plug tray can be repeatedly used after being thoroughly cleaned and sterilized, and the plastic plug tray is low in cost, light and convenient to use in a large batch. The size of the plug is matched with that of the black tiger seeds, and the large size can increase the amount of used matrix and increase the seedling raising cost; too small a hole tray size can lead to poor growth of the seedling root system, root nesting and influence on the seedling quality. Optionally, the plug is a 72-105 hole plastic plug, the diameter of the upper plug of the plastic plug is 3.2-4cm, the diameter of the lower plug is 1.2-2cm, the depth of the plug is 3.5-4.5cm, and the size of the plastic plug is 54 × 28 cm.
The black tiger seeds are cultured under dark light by arranging the light-shading object, the light shading is favorable for the black tiger seeds to germinate, and the light-shading object is selected from newspaper, non-woven fabrics or opaque plastic films, so that the black tiger seeds not only have the light-shading effect, but also have the moisturizing effect, and the substrate and the seeds are favorably kept at constant humidity.
And (3) management after sowing: controlling the temperature of the culture room or seedling shed at 20-27 deg.C and humidity at 70-90%, removing shade when seedling emergence rate reaches 25-35%, and culturing at normal temperature when seedling emergence rate reaches 65-75%.
Cultivate in cultivateing room or educate seedling shed and to prevent that the process of emerging of black tiger seed from receiving the influence of wind and rain factor, can also avoid the influence of mouse pest and insect pest, in addition, can also provide invariable temperature and humidity in cultivateing the room or educating seedling shed, under 70-90% humidity, black tiger seed can obtain sufficient moisture supply and can not lead to the condition that the seed rotten, guarantees that black tiger seed germinates smoothly.
Removing the shade when the seedling emergence rate reaches 25-35%, and culturing under day and night variation condition of natural light irradiation; when the seedling emergence rate of the seeds reaches 65-75%, the temperature of a culture room or a seedling raising shed is not controlled any more, and the seeds are cultured at normal temperature, so that the temperature of the seeds is changed along with the temperature change of day and night. At the same time, the humidity was maintained at 70-90% during the cultivation.
Transplanting: after 1-2 true leaves grow out from the seedling, transplanting the seedling and the matrix to a field.
The method can lead the black tiger to sprout about 45 days in advance, greatly shorten the germination and seedling time of the black tiger seeds, effectively avoid the adverse effects of rat damage, insect damage and the like, and lead the seedling emergence rate to be more than 90 percent. The improvement of the technology of the invention has important significance for improving the quality of the nursery stock for planting the black cutworms and promoting the rapid development of the black cutworm industry.
The present invention will be described in further detail with reference to the following examples, but the present invention is not limited thereto.
Example 1
The black tiger seed seedling raising method provided by the embodiment of the invention comprises the following steps:
seed selection: and (3) screening the seeds with full seeds, tan seed coats and luster from the collected black cutworm seeds, wherein the thousand seed weight of the seeds is 250 g.
Storing wet sand: and (3) storing the screened seeds in wet sand for 30 days, turning over the seeds once every 10 days, wherein the weight ratio of the seeds to the wet sand is 1:3.
Temperature change treatment: the seeds after storing in wet sand were treated at 5 deg.C for 14hr and at 25 deg.C for 10hr, respectively, and repeated 2 times.
Soaking treatment: soaking the seeds subjected to temperature change treatment in a seed pretreatment solution for 20hr, and then soaking in a 0.1% potassium permanganate solution for 15min, wherein the weight ratio of gibberellin to naphthylacetic acid in the seed pretreatment solution is 4:1, and the total solute concentration is 50 mg/L.
Sowing: in a culture room, sowing the soaked seeds in a 72-hole plastic hole tray with a matrix of water moss, wherein the hole upper caliber of the plastic hole tray is 4.0cm, the hole lower caliber is 2.0cm, the hole depth is 4.5cm, the size of the plastic hole tray is 54 x 28cm, watering the matrix thoroughly with clear water, covering the sown seeds with the water moss with the thickness of 0.4cm, spraying the clear water, and covering the covered water moss with newspaper.
And (3) management after sowing: controlling the temperature of the culture room at 20 ℃ and the humidity at 70%, removing newspaper when the seedling emergence of the seeds reaches 30%, and culturing at normal temperature when the seedling emergence of the seeds reaches 70%.
Transplanting: after 2 true leaves grow out from the seedling, transplanting the seedling and the matrix to a field.
Compared with the traditional method, the black tiger seeds of the embodiment emerge 45 days ahead of time, and the emergence rate is 92.5%.
Example 2
The black tiger seed seedling raising method provided by the embodiment of the invention comprises the following steps:
seed selection: and (3) screening the seeds with full seeds, tan seed coats and luster from the collected black cutworm seeds, wherein the thousand seed weight of the seeds is 220 g.
Storing wet sand: and (3) storing the screened seeds in wet sand for 25 days, turning over the seeds once every 7 days, wherein the weight ratio of the seeds to the wet sand is 1: 2.5.
Temperature change treatment: the seeds after storing in wet sand were treated at 1 deg.C for 13hr and at 20 deg.C for 9hr, respectively, and repeated 2 times.
Soaking treatment: soaking the seeds subjected to temperature change treatment in a seed pretreatment solution for 20hr, and then soaking in a 0.1% potassium permanganate solution for 15min, wherein the weight ratio of gibberellin to naphthylacetic acid in the seed pretreatment solution is 3:1, and the total solute concentration is 40 mg/L.
Sowing: in a seedling raising shed, seeds after soaking treatment are sowed in 105-hole plastic hole trays with water moss as a matrix, the hole upper caliber of each plastic hole tray is 3.2cm, the hole lower caliber of each plastic hole tray is 1.2cm, the hole depth is 3.5cm, the size of each plastic hole tray is 54 x 28cm, the matrix is thoroughly watered with clear water, the sowed seeds are covered with the water moss with the thickness of 0.4cm and sprayed with the clear water, and the covered water moss is covered with newspaper.
And (3) management after sowing: controlling the temperature of the seedling raising shed to be 20 ℃ and the humidity to be 70%, removing newspaper when the seedling emergence rate of the seeds reaches 25%, and culturing at normal temperature when the seedling emergence rate of the seeds reaches 65%.
Transplanting: after 1 true leaf of the seedling grows out, transplanting the seedling and the matrix to a field.
Compared with the traditional method, the black tiger seeds of the embodiment emerge 35 days in advance, and the emergence rate is 90%.
Example 3
The black tiger seed seedling raising method provided by the embodiment of the invention comprises the following steps:
seed selection: and (3) screening the seeds with plump seeds, tan seed coats and luster from the collected black cutworm seeds, wherein the thousand seed weight of the seeds is 245 g.
Storing wet sand: and (3) storing the screened seeds in wet sand for 26 days, turning over the seeds once every 8 days, wherein the weight ratio of the seeds to the wet sand is 1: 2.6.
Temperature change treatment: the seeds after storing in wet sand were treated at 2 deg.C for 14hr and at 21 deg.C for 10hr, respectively, and repeated 3 times.
Soaking treatment: soaking the seeds subjected to temperature change treatment in a seed pretreatment solution for 21hr, and then soaking in a 0.15% potassium permanganate solution for 16min, wherein the weight ratio of gibberellin to naphthylacetic acid in the seed pretreatment solution is 3.1:1, and the total solute concentration is 41 mg/L.
Sowing: in a culture chamber, sowing the soaked seeds in a 105-hole plastic hole tray with a matrix of water moss, wherein the hole upper caliber of the plastic hole tray is 3.3cm, the hole lower caliber is 1.3cm, the hole depth is 3.6cm, the size of the plastic hole tray is 54 x 28cm, watering the matrix with clean water, covering the sown seeds with the water moss with the thickness of 0.5cm, spraying the clean water, and covering the covered water moss with non-woven fabrics.
And (3) management after sowing: controlling the temperature of the culture room at 21 deg.C and humidity at 71%, removing the non-woven fabric when the seedling emergence rate of the seeds reaches 26%, and culturing at normal temperature when the seedling emergence rate of the seeds reaches 66%.
Transplanting: after 2 true leaves grow out from the seedling, transplanting the seedling and the matrix to a field.
Compared with the traditional method, the black tiger seeds of the embodiment emerge 36 days in advance, and the emergence rate is 90.5%.
Example 4
The black tiger seed seedling raising method provided by the embodiment of the invention comprises the following steps:
seed selection: and (3) screening the seeds with plump seeds, tan seed coats and luster from the collected black cutworm seeds, wherein the thousand seed weight of the seeds is 230 g.
Storing wet sand: and (3) storing the screened seeds in wet sand for 27 days, turning over the seeds once every 9 days, wherein the weight ratio of the seeds to the wet sand is 1: 2.7.
Temperature change treatment: the seeds after storing in wet sand were treated at 3 deg.C for 15hr and at 22 deg.C for 11hr, respectively, and repeated 2 times.
Soaking treatment: soaking the seeds subjected to temperature change treatment in a seed pretreatment solution for 22hr, and then soaking in a 0.2% potassium permanganate solution for 17min, wherein the weight ratio of gibberellin to naphthylacetic acid in the seed pretreatment solution is 4:1, and the total solute concentration is 42 mg/L.
Sowing: in a seedling raising shed, seeds after soaking are sowed in a 72-hole plastic hole tray with the matrix of water moss, the hole upper caliber of the plastic hole tray is 3.9cm, the hole lower caliber is 1.6cm, the hole depth is 3.7cm, the size of the plastic hole tray is 54 x 28cm, the matrix is thoroughly watered with clear water, the sowed water moss with the thickness of 0.6cm is covered and sprayed with clear water, and an opaque plastic film is covered on the covered water moss.
And (3) management after sowing: controlling the temperature of the seedling raising shed to be 22 ℃ and the humidity to be 72%, removing the opaque plastic film when the seedling emergence rate of the seeds reaches 27%, and culturing at normal temperature when the seedling emergence rate of the seeds reaches 67%.
Transplanting: after 1 true leaf of the seedling grows out, transplanting the seedling and the matrix to a field.
Compared with the traditional method, the black tiger seeds of the embodiment emerge 37 days earlier, and the emergence rate is 91%.
Example 5
The black tiger seed seedling raising method provided by the embodiment of the invention comprises the following steps:
seed selection: and (3) screening the seeds with full seeds, tan seed coats and luster from the collected black cutworm seeds, wherein the thousand seed weight of the seeds is 255 g.
Storing wet sand: and (3) storing the screened seeds in wet sand for 28 days, turning over the seeds once every 10 days, wherein the weight ratio of the seeds to the wet sand is 1: 2.8.
Temperature change treatment: the seeds after storing in wet sand were treated at 4 deg.C for 13hr and at 23 deg.C for 9hr, respectively, and repeated 3 times.
Soaking treatment: soaking the seeds subjected to temperature change treatment in a seed pretreatment solution for 23hr, and then soaking in a 0.1% potassium permanganate solution for 18min, wherein the weight ratio of gibberellin to naphthylacetic acid in the seed pretreatment solution is 3:1, and the concentration of total solute is 43 mg/L.
Sowing: in a culture room, sowing the soaked seeds in a 105-hole plastic hole tray with a matrix of water moss, wherein the hole upper caliber of the plastic hole tray is 3.5cm, the hole lower caliber is 1.5cm, the hole depth is 3.8cm, the size of the plastic hole tray is 54 x 28cm, watering the matrix thoroughly with clear water, covering the sown seeds with the water moss with the thickness of 0.4cm, spraying the clear water, and covering the covered water moss with newspaper.
And (3) management after sowing: controlling the temperature of the culture room at 23 ℃ and the humidity at 73%, removing newspaper when the seedling emergence of the seeds reaches 28%, and culturing at normal temperature when the seedling emergence of the seeds reaches 68%.
Transplanting: after 2 true leaves grow out from the seedling, transplanting the seedling and the matrix to a field.
Compared with the traditional method, the black tiger seeds of the embodiment emerge 38 days in advance, and the emergence rate is 91.5%.
Example 6
The black tiger seed seedling raising method provided by the embodiment of the invention comprises the following steps:
seed selection: and (3) screening the seeds with full seeds, tan seed coats and luster from the collected black cutworm seeds, wherein the thousand seed weight of the seeds is 240 g.
Storing wet sand: and (3) storing the screened seeds in wet sand for 29 days, turning over the seeds once every 7 days, wherein the weight ratio of the seeds to the wet sand is 1: 2.9.
Temperature change treatment: the seed after storing in wet sand is treated at 5 deg.C for 14hr and at 24 deg.C for 10hr, respectively, and repeated for 2 times.
Soaking treatment: soaking the seeds subjected to temperature change treatment in a seed pretreatment solution for 24hr, and then soaking in a 0.15% potassium permanganate solution for 19min, wherein the weight ratio of gibberellin to naphthylacetic acid in the seed pretreatment solution is 4:1, and the total solute concentration is 44 mg/L.
Sowing: in a seedling raising shed, seeds after soaking are sowed in a 72-hole plastic hole tray with the matrix of water moss, the hole upper caliber of the plastic hole tray is 3.9cm, the hole lower caliber is 1.6cm, the hole depth is 3.9cm, the size of the plastic hole tray is 54 x 28cm, the matrix is thoroughly watered with clear water, the sowed water moss with the thickness of 0.5cm is covered and sprayed with clear water, and non-woven fabrics are covered on the covered water moss.
And (3) management after sowing: controlling the temperature of the seedling raising shed at 24 ℃ and the humidity at 74%, removing the non-woven fabrics when the seedling emergence of the seeds reaches 29%, and culturing at normal temperature when the seedling emergence of the seeds reaches 69%.
Transplanting: after 1 true leaf of the seedling grows out, transplanting the seedling and the matrix to a field.
Compared with the traditional method, the black tiger seeds of the embodiment emerge 39 days in advance, and the emergence rate is 92%.
Example 7
The black tiger seed seedling raising method provided by the embodiment of the invention comprises the following steps:
seed selection: and (3) screening the seeds with plump seeds, tan seed coats and luster from the collected black cutworm seeds, wherein the thousand seed weight of the seeds is 245 g.
Storing wet sand: and (3) storing the screened seeds in wet sand for 30 days, turning over the seeds once every 8 days, wherein the weight ratio of the seeds to the wet sand is 1:3.
Temperature change treatment: the seeds after storing in wet sand were treated at 6 deg.C for 15hr and at 25 deg.C for 11hr, respectively, and repeated 3 times.
Soaking treatment: soaking the seeds subjected to temperature change treatment in a seed pretreatment solution for 20hr, and then soaking in a 0.2% potassium permanganate solution for 20min, wherein the weight ratio of gibberellin to naphthylacetic acid in the seed pretreatment solution is 3.5:1, and the total solute concentration is 45 mg/L.
Sowing: in a culture chamber, sowing the soaked seeds in a 105-hole plastic hole tray with a matrix of water moss, wherein the hole upper caliber of the plastic hole tray is 3.5cm, the hole lower caliber is 1.5cm, the hole depth is 4cm, the size of the plastic hole tray is 54 x 28cm, watering the matrix thoroughly with clear water, covering the sown seeds with the water moss with the thickness of 0.6cm, spraying clear water, and covering the covered water moss with an opaque plastic film.
And (3) management after sowing: controlling the temperature of the culture room at 25 deg.C and humidity at 75%, removing the opaque plastic film when the seedling emergence rate of the seeds reaches 30%, and culturing at room temperature when the seedling emergence rate of the seeds reaches 70%.
Transplanting: after 2 true leaves grow out from the seedling, transplanting the seedling and the matrix to a field.
Compared with the traditional method, the black tiger seeds of the embodiment emerge 40 days earlier, and the emergence rate is 92.5%.
Example 8
The black tiger seed seedling raising method provided by the embodiment of the invention comprises the following steps:
seed selection: and (3) screening the seeds with full seeds, tan seed coats and luster from the collected black cutworm seeds, wherein the thousand seed weight of the seeds is 250 g.
Storing wet sand: and (3) storing the screened seeds in wet sand for 31 days, turning over the seeds once every 9 days, wherein the weight ratio of the seeds to the wet sand is 1: 3.1.
Temperature change treatment: the seeds after storing in wet sand were treated at 7 deg.C for 13hr and at 26 deg.C for 9hr, respectively, and repeated 2 times.
Soaking treatment: soaking the seeds subjected to temperature change treatment in a seed pretreatment solution for 21hr, and then soaking in a 0.1% potassium permanganate solution for 21min, wherein the weight ratio of gibberellin to naphthylacetic acid in the seed pretreatment solution is 4:1, and the total solute concentration is 46 mg/L.
Sowing: in a seedling raising shed, seeds after soaking treatment are sowed in a 72-hole plastic hole tray with the matrix of water moss, the hole upper caliber of the plastic hole tray is 3.8cm, the hole lower caliber is 1.8cm, the hole depth is 4.1cm, the size of the plastic hole tray is 54 x 28cm, the matrix is thoroughly watered with clear water, the sowed water moss with the thickness of 0.4cm is covered and the clear water is sprayed, and newspaper is covered on the covered water moss.
And (3) management after sowing: controlling the temperature of the seedling raising shed to be 26 ℃ and the humidity to be 76%, removing newspaper when the seedling emergence of the seeds reaches 31%, and culturing at normal temperature when the seedling emergence of the seeds reaches 71%.
Transplanting: after 1 true leaf of the seedling grows out, transplanting the seedling and the matrix to a field.
Compared with the traditional method, the black tiger seeds of the embodiment emerge 41 days in advance, and the emergence rate is 90%.
Example 9
The black tiger seed seedling raising method provided by the embodiment of the invention comprises the following steps:
seed selection: and (3) screening the seeds with full seeds, tan seed coats and luster from the collected black cutworm seeds, wherein the thousand seed weight of the seeds is 235 g.
Storing wet sand: and (3) storing the screened seeds in wet sand for 32 days, turning over the seeds once every 10 days, wherein the weight ratio of the seeds to the wet sand is 1: 3.2.
Temperature change treatment: the seeds after storing in wet sand were treated at 8 deg.C for 14hr and at 27 deg.C for 10hr, respectively, and repeated 3 times.
Soaking treatment: soaking the seeds subjected to temperature change in a seed pretreatment solution for 22hr, and then soaking in a 0.15% potassium permanganate solution for 22min, wherein the weight ratio of gibberellin to naphthylacetic acid in the seed pretreatment solution is 3:1, and the total solute concentration is 47 mg/L.
Sowing: in a culture chamber, sowing the soaked seeds in a 105-hole plastic hole tray with a matrix of water moss, wherein the hole upper caliber of the plastic hole tray is 3.5cm, the hole lower caliber is 1.5cm, the hole depth is 4.0cm, the size of the plastic hole tray is 54 x 28cm, watering the matrix with clean water, covering the sown seeds with the water moss with the thickness of 0.5cm, spraying the clean water, and covering the covered water moss with non-woven fabrics.
And (3) management after sowing: controlling the temperature of the culture chamber at 27 deg.C and humidity at 77%, removing the non-woven fabric when seedling emergence rate reaches 32%, and culturing at room temperature when seedling emergence rate reaches 72%.
Transplanting: after 2 true leaves grow out from the seedling, transplanting the seedling and the matrix to a field.
Compared with the traditional method, the black tiger seeds of the embodiment emerge 42 days in advance, and the emergence rate is 90.5%.
The above-described embodiments of the present invention should not be construed as limiting the scope of the present invention.
Claims (8)
1. A black tiger seed seedling raising method is characterized by comprising the following steps:
seed selection: screening seeds which are full in seeds, brown in seed coats and glossy from the collected black tiger seeds;
storing wet sand: storing the screened seeds in wet sand for 25-35 days, and turning over the seeds once every 7-10 days;
temperature change treatment: treating the seeds stored in wet sand at 1-10 deg.C for 13-15hr and at 20-30 deg.C for 9-11hr, repeating for 2-3 times;
soaking treatment: soaking the seeds subjected to temperature change treatment in a seed pretreatment solution for 20-24hr, and then soaking in a 0.1% -0.2% potassium permanganate solution for 15-30min, wherein the seed pretreatment solution is a mixed solution composed of gibberellin and naphthylacetic acid;
sowing: sowing the soaked seeds in a hole tray with a matrix of water moss in a culture room or a seedling shed, thoroughly watering the matrix with clear water, covering the sowed seeds with 0.4-0.6cm thick water moss, spraying clear water, and arranging a shade on the covered water moss;
and (3) management after sowing: controlling the temperature of the culture room or seedling shed at 20-27 deg.C and humidity of 70-90%, removing shade when seedling emergence rate reaches 25-35%, and culturing at normal temperature when seedling emergence rate reaches 65-75%;
transplanting: after 1-2 true leaves grow out from the seedling, transplanting the seedling and the matrix to a field.
2. The Tiger seed seedling raising method according to claim 1, wherein the thousand seed weight of the screened seed is not less than 220 g.
3. A method for growing black cutworm seeds according to claim 1, wherein the weight ratio of the seeds to the wet sand is 1 (2.5-3.5) when the wet sand is stored.
4. A method for growing black cutworm seeds according to claim 3, wherein the weight ratio of the seeds to the wet sand is 1:3 when the wet sand is stored.
5. A method for growing black cutworm seeds according to claim 1, wherein the weight ratio of gibberellin to naphthylacetic acid in the seed pretreatment liquid is (3-4):1, and the total solute concentration is 40-50 mg/L.
6. A method for growing black cutworm seeds as claimed in claim 5, wherein the weight ratio of gibberellin to naphthylacetic acid in the seed pretreatment liquid is 4:1, and the total solute concentration is 50 mg/L.
7. The black cutworm seed seedling raising method according to claim 1, wherein the aperture disk is a plastic aperture disk with 72-105 holes, the aperture of the upper aperture of the plastic aperture disk is 3.2-4cm, the aperture of the lower aperture of the plastic aperture disk is 1.2-2cm, the depth of the aperture is 3.5-4.5cm, and the size of the plastic aperture disk is 54 x 28 cm.
8. A black tiger seed seedling method according to claim 1, wherein the shade is newspaper, non-woven fabric or opaque plastic film.
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| CN105284532A (en) * | 2015-10-24 | 2016-02-03 | 高渐飞 | Seedling cultivation method for wild fruit kadsurae coccinea (Lem.)A.C.Smith |
| CN107493723A (en) * | 2017-08-07 | 2017-12-22 | 六安栀子农业科技有限公司 | A kind of processing method of salvia seeds |
| CN107801543A (en) * | 2017-09-27 | 2018-03-16 | 唐光考 | The implantation methods of black tiger |
| CN108464178A (en) * | 2018-03-13 | 2018-08-31 | 贵州省山地资源研究所 | A kind of epicormic branch cutting method of kadsurae coccineae |
| CN108496668A (en) * | 2018-02-10 | 2018-09-07 | 荔浦县万家兴果蔬专业合作社 | A kind of method for culturing seedlings of clausenae Lansium |
| CN109220049A (en) * | 2018-09-20 | 2019-01-18 | 四川盛世佳禾农业开发有限公司 | A kind of seed accelerating germination method |
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|---|---|---|---|---|
| CN105284532A (en) * | 2015-10-24 | 2016-02-03 | 高渐飞 | Seedling cultivation method for wild fruit kadsurae coccinea (Lem.)A.C.Smith |
| CN107493723A (en) * | 2017-08-07 | 2017-12-22 | 六安栀子农业科技有限公司 | A kind of processing method of salvia seeds |
| CN107801543A (en) * | 2017-09-27 | 2018-03-16 | 唐光考 | The implantation methods of black tiger |
| CN108496668A (en) * | 2018-02-10 | 2018-09-07 | 荔浦县万家兴果蔬专业合作社 | A kind of method for culturing seedlings of clausenae Lansium |
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