CN109362545A - A kind of iris tissue-cultured seedling florescence control method - Google Patents
A kind of iris tissue-cultured seedling florescence control method Download PDFInfo
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Abstract
The invention discloses a kind of iris tissue-cultured seedling florescence control methods, method includes the following steps: 1. wrapping up iris seedling root system with sphagna and being placed in 1.5L nutritive cube, culture starts to apply fertilizer when having new root to grow in greenhouse；2. root system changes 3.5 cun of nutritive cubes into when coiling full in sphagna, fertilising in every 10 days is primary after changing basin 10 days；3. the acid solution that falls off pours Phalaenopsis plants root to promote Phalaenopsis plants suspend mode；4. 120 days beginning floral bud inductions before estimated list marketing, Flower induction early period is with GA3 solution and KH2PO4Solution pours Phalaenopsis plants root；The Flower induction later period pours Phalaenopsis plants root with compound fertilizer's solution；After the stage natural lighting, continue to carry out iris the red blue light composite light source irradiation of LED；5. flower bud differentiation period pours Phalaenopsis plants root with 6-BA solution.This method regulates and controls the iris tissue-cultured seedling florescence, and to obtain, flowering time is controllable, more neat and gorgeous iris of blooming.
Description
Technical field
The present invention relates to florescence control field, especially a kind of iris tissue-cultured seedling florescence control method.
Technical background
Iris (Phalaenopsis Bl.Spp.) is because it is spent greatly, and the florescence is long, and pattern is gorgeous, and color is abundant, flower-shape beauty
Beautiful uniqueness as butterfly dances lightly, thus gains the name, has the laudatory title of " orchid queen " in hot bandwidth, be on China flowers market
Popular high-grade indoor pot flowers.
The source area of iris is hylaeion hypotropicum area, and happiness is moist, cold Keep away from sun, and optimum growth temp is 18~28
DEG C, optimum growh humidity is 60%~90%.It is bloomed due to iris and needs specific condition, such as temperature, illumination, hormone,
Therefore need to accordingly be regulated and controled when artificial culture.
Florescence combined regulation and control method of macranthus Hui orchid is disclosed in Patent No. 200710064032.8, it is main by going up a hill more
The mode in summer carries out temperature regulation, and is used cooperatively plant growth regulator to carry out the regulation hybrid cymbidium florescence；Party legal person
Work cost and transportation cost are high, and the temperature in more summer of going up a hill is difficult to control, and leads to the florescence for being difficult to obtain neat and consistent.The patent No.
To disclose the method for Bowring cattleya blooming-period adjustment and control in 200810241084.2, mainly carried out by temperature, illumination and mineral element
Regulation；This method is handled without suspend mode, and temperature, illumination and the mineral element during entire florescence control are without becoming
Change, therefore, it is difficult to obtain the florescence of neat and consistent.A kind of hybrid cymbidium florescence is disclosed in Patent No. 201210167208.3
Regulation method is mainly regulated and controled by plant growth regulator, mineral element；It is difficult to ensure gorgeous pattern.Patent No.
A kind of method at regulation sword-leaved cymbidium florescence is disclosed in 201510932351.0, mainly passes through plant growth regulator, temperature, mineral
Element is regulated and controled, it is difficult to guarantee gorgeous pattern.
Summary of the invention
In order to solve the above technical problem, the present invention provides a kind of iris tissue-cultured seedling florescence control methods, pass through adjusting
The many factors such as temperature, light application time, light quality, mineral element and plant growth regulator, to obtain, flowering time is controllable, opens
The more neat and gorgeous iris of flower.
The technical solution adopted by the present invention is that a kind of iris tissue-cultured seedling florescence control method, comprising the following steps:
1) transplanting takes out iris tissue-cultured seedling from bottle, cleans culture medium, then impregnates root with Bravo solution, then
Iris root system is wrapped up with the sphagna sterilized, is placed in nutritive cube and fills culture substrate, cultivated；To iris tissue culture
Start to apply fertilizer when seedling has new root to grow；
2) basin is changed when the root system of iris tissue-cultured seedling coils full in sphagna, is changed the nutritive cube bigger than in step 1), is filled out
Full culture substrate, continues to cultivate；Start to apply fertilizer after changing basin 10 days；
3) when suspend mode iris tissue-cultured seedling has 4 or more health into age leaf, the acid solution that falls off pours Phalaenopsis plants root
Portion, to promote Phalaenopsis plants suspend mode；
4) 120 days beginning floral bud inductions before the estimated list marketing of Flower induction phase, Flower induction early period is every 10 days with dense
Spend the KH of the GA3 solution for being 400mg/L and 0.1%2PO4Solution pouring Phalaenopsis plants root is each primary, sprays blade face water daily
Once, which cultivates 20-30 days；The Flower induction later period poured Phalaenopsis plants root with compound fertilizer's solution every 10 days, should
Stage cultivates 40-60 days；
5) flower bud differentiation period bennet it is long to 8-12cm when, be that 400mg/L 6-BA solution pours Phalaenopsis plants root with concentration
Portion；Phalaenopsis plants root was poured with compound fertilizer's solution every 10 days during this period；When bennet it is long to 15~20cm when, need to it
It is fixed；Meanwhile the phototropic face of constantly adjustment petal, it can in uniform light；The stage cultivates 30-50 days.When suspend mode
It is 30mg/L with the acid solution that falls off.
Further, in step 1), taking-up when iris tissue-cultured seedling has 3-4 piece leaf, 2-4 item root, root long 2-3cm；Hundred
Soaking time is 30-60s in bacterium clear solution, to sterilize iris tissue-cultured seedling root.
Further, in step 1), culture substrate is using castanopsis fissa bark pieces, peat soil, decomposed husk and bagasse
Ash is mixed by weight 60-65:10-20:10-20:5-8；Matrix in step 2) is using castanopsis fissa bark block, peat
50-60 by weight parts: 20-25 part: 20-25 parts of native and decomposed husk mixes.
Step 1) and the castanopsis fissa bark in step 2) culture substrate are in different size, are primarily due to the butterfly of step 1)
Blue tissue-cultured seedling root system is also undeveloped, and root system needs closer contact castanopsis fissa bark and absorbs corticole nutrient for its life
It is long, therefore castanopsis fissa bark more fine crushing is used as matrix main component；The iris tissue-cultured seedling root system of step 2) has compared
It is more developed, need the matrix of water-permeable and air permeable to prevent iris tissue-cultured seedling root system from rotting, therefore use relatively large castanopsis fissa bark
As matrix main component.In addition, the culture substrate bagasse ash much a kind of than step 2) in step 1), bagasse ash contains higher
Sylvite can promote iris tissue-cultured seedling Stem growth.In step 1), by using a certain proportion of bagasse ash, matrix is improved
The content of middle potassium ion, to promote iris tissue-cultured seedling Stem growth；In step 2), bagasse ash is not used, has used high nitrogen multiple
Hefei stores energy to promote the growth of iris tissue culture seedling leaf to output gorgeous flower.
Wherein castanopsis fissa bark pieces, size is 1-2cm (length) × 0.5-1cm (width), and keeps EC value steady after decomposed
It is scheduled between 0.4-0.8ms/cm；Castanopsis fissa bark block, size are 3-8cm (length) × 1.1-3cm (width), and after decomposed
Stablize EC value between 0.4-0.8ms/cm；Decomposed husk, be make after decomposed EC value stablize 0.5-0.9ms/cm it
Between；Bagasse ash is that sugar refinery bagasse makees boiler oil, the lime-ash generated after burning.
Further, it being placed in the greenhouse with sunshade net and cultivates when culture in step 1), cultivation temperature is 22-28 DEG C,
Natural lighting, intensity of illumination 4000-8000lux, light application time 12h/d, relative air humidity 70%-90%；Step
When rapid 2) middle culture, continue to be placed in the greenhouse with sunshade net and cultivate, cultivation temperature is 23-30 DEG C, natural lighting, illumination
Intensity is 8000-10000lux, light application time 12h/d, relative air humidity 60%-80%；In step 3) when suspend mode, training
Supporting temperature is 18-26 DEG C, intensity of illumination 6000-8000lux, light application time 10h/d, relative air humidity 60%-
80%.
Step 1) in step 2) cultivation temperature and intensity of illumination gradually increase, main function be promote iris tissue culture
Seedling growth；And step 3), to promote iris tissue-cultured seedling suspend mode, is adjusted by reducing cultivation temperature and intensity of illumination with step 4)
It controls the cultivation temperature used and intensity of illumination forms larger difference, regulating effect is good, reaches uniformity height of blooming, and pattern is gorgeous
Purpose.
Further, in step 1) when fertilising, the compound fertilizer of the N:P:K=10:26:15 of 4000 times of dilution is applied, every 10 days
It applies primary；In step 2) when fertilising, the compound fertilizer of the N:P:K=26:12:12 of 1000 times of dilution is applied, is applied within every 10 days primary.
Phosphate fertilizer content in step 1) is higher, can promote iris tissue-cultured seedling root system fast-growth；And the nitrogen in step 2)
Fertile content is higher, can promote iris tissue-cultured seedling leaf growth, stores energy to output gorgeous flower.
Further, in step 4), daily natural lighting is maintained at 15000-25000lux, and when natural lighting deficiency is used
Incandescent lamp light filling, natural lighting time are 12h/d；After natural lighting, continue to carry out the irradiation of LED composite light source to iris
Handle 2-4h；Night cultivation temperature is 16-18 DEG C, and cultivation temperature on daytime is 25-28 DEG C, relative air humidity 70%-80%.
Further, the LED composite light source, it is compound to be that two kinds of monochromatic light of LED feux rouges and LED blue light are combined into LED
Light source, wherein the wavelength of LED feux rouges is 630nm；The wavelength of LED blue light is 450-480nm, and feux rouges and blue light match R:B (light quantity
Ratio)=4:1.
Further, in step 5), daily natural lighting is maintained at 12000-15000lux, with white when natural light deficiency
Vehement lamp light filling, natural lighting time are 12 hours；After natural light, continue to carry out iris LED blue light illumination processing 3-
4h；Cultivation temperature is 18-26 DEG C, relative air humidity 60%-70%.
Further, the LED blue light；The wavelength of its LED blue light is 450-480nm.
Further, the compound fertilizer solution in step 4) be N:P:K=10:20:20 and dilute 2000 times obtain it is compound
Fertile solution；Compound fertilizer solution in step 5) is N:P:K=10:25:15 and dilutes 2000 times of obtained compound fertilizer's solution.
The releasing of bud suspend mode, bud breed and bloom and suffer from the influence of plant endogenous hormones.But pass through tune
Section temperature can also break the suspend mode of flowers, and the formation and differentiation for promoting bud are to regulate and control flowering of plant；And extend plant illumination
Time transformation of the plant from nutrient growth to reproductive growth also may be implemented, and go on smoothly bud differentiation；Light quality also can shadow
Plant flower development is rung, the red compound light quality of indigo plant is conducive to the formation of bud, and blue light has facilitation to the differentiation of plant bud.In addition,
Flowering of plant can also be regulated and controled by adjusting mineral element.
Therefore, in the formation and atomization of plant bud, will receive temperature, light application time, light quality, mineral element with
And the influence of many factors such as plant growth regulator.In the formation and atomization of different plant buds, when to temperature, illumination
Between, the sensibility of light quality, mineral element and plant growth regulator it is different, the florescence may finally be caused to be difficult to control, bloom
It is irregular, pattern is not gorgeous, finally affect the ornamental value of flowers.
It innovative point of the invention and has the beneficial effect that:
1. coordinating the regulation iris florescence by Multiple factors, so that iris florescence is controllable.Specific manifestation are as follows:
1. the Flower induction phase promotes flower_bud formation using day and night temperature；
2. light application time shortens to 10h/d from 12h/d to promote Phalaenopsis plants suspend mode；
3. Flower induction phase and flower bud differentiation period, by extend illumination, i.e., continue after natural lighting to iris into
Row LED light source treatment with irradiation, to promote flower_bud formation and differentiation；
4. the Flower induction phase continues to carry out the red blue light composite light source treatment with irradiation of LED to iris after natural lighting,
To promote flower_bud formation；Flower bud differentiation period continues after natural light to carry out the processing of LED blue light illumination to iris, to promote
Bud differentiation；
5. period the last period Flower induction phase has used the fertilizer of low-phosphorous low potassium, and the Flower induction phase has used the high potassium of high phosphorus
Fertilizer, the two form difference to promote flower_bud formation；
6. pouring Phalaenopsis plants root with GA3 solution to promote Phalaenopsis plants flower_bud formation；It is poured with 6-BA solution
Phalaenopsis plants root is to promote Phalaenopsis plants bud differentiation.
2. regulating and controlling by Multiple factors, iris is set to bloom more neat.After nutrient growth, coordinated by three factors
Regulation iris enters dormant period, regulates and controls flower_bud formation again when needed, more neat to achieve the purpose that bloom.Promote butterfly
Orchid enters suspend mode method particularly includes: with falling off, acid solution pours Phalaenopsis plants root, and intensity of illumination is reduced to 6000-
8000lux, light application time shorten to 10h/d.
3. regulating and controlling by Multiple factors, iris is set to bloom more gorgeous.Firstly, during iris nutrient growth, training
Feeding matrix is that sphagna, castanopsis fissa bark, peat soil, husk and bagasse ash etc. mix；The matrix is iris tissue-cultured seedling root
Portion's growth creates loose ventilative environment and suitable pH value, and the iris grown on the substrate is healthy and strong, and leaf color is dark green, is
The florescence that iris outputs gorgeous pattern and overlength provides guarantee.Secondly, the Flower induction phase has used day and night temperature, can make
The flower that iris is outputed is more gorgeous.
Specific embodiment
The present invention is further illustrated below with reference to embodiment, but the scope of protection of present invention is not limited to implement
The range of example statement.
Embodiment 1:
It was carried out in the flowers temperature canopy of Liuzhou in 2016, is florescence control implementation with butterfly orchid variety " firebird "
Example.Specific step is as follows:
(1) 3-4 piece leaf, 2-4 item root, root long about 2- iris tissue culture transplantation of seedlings: are grown to " firebird " iris tissue-cultured seedling
When 3cm, iris seedling is taken out from vial, clean the culture medium of root, impregnates " firebird " butterfly with Bravo solution
After 30 seconds, Bravo solution is that the Bravo pulvis for being 75% by active constituent content dilutes 500 times by quality for blue seedling root
It obtains afterwards, wraps up iris seedling root system with the sphagna sterilized, be placed in 1.5 cun of nutritive cubes；And it is broken with castanopsis fissa bark
The matrix that piece, peat soil, decomposed husk and bagasse ash mix fills up nutritive cube, which is 60 parts according to mass parts meter
Castanopsis fissa bark pieces, 20 parts of peat soil, 15 parts of decomposed husk, 5 parts of bagasse ash；It is placed on after transplanting with sunshade net
Greenhouse in culture, cultivation temperature is 22-28 DEG C, and natural lighting, intensity of illumination 4000-8000lux, light application time is
12h/d, relative air humidity 70%-90%；
(2) apply fertilizer after transplanting: iris tissue-cultured seedling starts to apply fertilizer when having new root to grow, and applies the N:P:K=of 4000 times of dilution
The compound fertilizer of 10:26:15 applies primary for every 10 days；
(3) iris tissue-cultured seedling changes basin: when the root system of iris tissue-cultured seedling coils full in sphagna, in 3.5 cun of nutrition
Castanopsis fissa bark block about 3-4cm after the pad fermentation of alms bowl bottom is thick, is put into the iris tissue-cultured seedling after having shelled 1.5 cun of nutritive cubes, then
Nutritive cube is filled up with the matrix that castanopsis fissa bark block, peat soil and decomposed husk mix, the matrix is according to mass parts meter, and 50
The castanopsis fissa bark pieces of part, 25 parts of peat soil, 25 parts of husk；Continue to be placed in the greenhouse with sunshade net after transplanting and train
It supports, cultivation temperature is 23-30 DEG C, natural lighting, intensity of illumination 8000-10000lux, light application time 12h/d, air
Relative humidity is 60%-80%；
(4) it applies fertilizer after changing basin: after iris tissue-cultured seedling changes basin 10 days, applying the N:P:K=26:12:12's of 1000 times of dilution
Compound fertilizer applies primary for every 10 days；
(5) promote Phalaenopsis plants suspend mode: when iris tissue-cultured seedling has 4 or more health into age leaf, with 30mg/L's
The acid solution that falls off pours Phalaenopsis plants root, which is 18-26 DEG C, intensity of illumination 6000-8000lux,
Light application time is 10h/d, relative air humidity 60%-80%, to promote Phalaenopsis plants suspend mode；
(6) the Flower induction phase:
1. Flower induction early period: in September, 2017 starts floral bud induction, and daily natural lighting is maintained at 15000-
25000lux, with incandescent lamp light filling when natural lighting deficiency, the natural lighting time is 12h/d；After natural lighting, continue pair
Iris carries out LED composite light source treatment with irradiation 2h, and wherein feux rouges and blue light match R:B (light quantity ratio)=4:1；Night culture
Temperature is 16-18 DEG C, and cultivation temperature on daytime is 25-28 DEG C, relative air humidity 70%-80%；And during this period every 10 days
With concentration be 400mg/L GA3 solution and 0.1% KH2PO4It is each primary that solution pours Phalaenopsis plants root；For suitable control
It is primary to spray blade face water daily for culture substrate humidity；The stage cultivates 30 days；
2. the Flower induction later period: daily natural lighting is maintained at 15000-25000lux, with white when natural lighting deficiency
Vehement lamp light filling, natural lighting time are 12h/d；After natural lighting, continue to carry out at the irradiation of LED composite light source iris
2h is managed, wherein feux rouges and blue light match R:B (light quantity ratio)=4:1；Night cultivation temperature be 16-18 DEG C, daytime cultivation temperature
It is 25-28 DEG C, relative air humidity 70%-80%；During this period every 10 days with 2000 times of N:P:K=10:20:20 of dilution
Compound fertilizer's solution pour Phalaenopsis plants root；The stage cultivates 48 days；
(7) flower bud differentiation period: bennet it is long to 8-12cm when, be that 400mg/L 6-BA solution pours Phalaenopsis plants with concentration
Root；Daily natural lighting is maintained at 12000-15000lux, with incandescent lamp light filling, natural lighting time when natural light deficiency
It is 12 hours；After natural light, continue to carry out iris LED blue light illumination processing 4h；Cultivation temperature is 18-26 DEG C, empty
Gas relative humidity is 60%-70%；During this period every 10 days compound fertilizer's solution with 2000 times of dilution of N:P:K=10:25:15
Pour Phalaenopsis plants root；When bennet it is long to 15~20cm when, need that it is fixed, can remain upright
Growth；Meanwhile the phototropic face of petal is constantly adjusted, it can in uniform light；The stage cultivates 42 days.
On December 31st, 2017, the flower and unopened bud that statistics 200 basins " firebird " iris has opened are total
2487, average every plant of " firebird " iris has 12.4 (standard deviations 1.41).A number of wherein blooming has 1572, average every
Strain " firebird " iris has 7.9 openings (standard deviation 0.98)；Unopened bud has 915, average every plant of " firebird " butterfly
Phalaenopsis has 4.6 not open (standard deviations 0.67)；Flowering rate reaches 63.2%.Flower of the present invention to " firebird " iris
Phase is controllable, and " firebird " iris is bloomed simultaneously, and uniformity is high, and pattern is gorgeous, ornamental value with higher.
Embodiment 2:
It was carried out in the flowers temperature canopy of Liuzhou in 2016, is florescence control implementation with butterfly orchid variety " big capsicum "
Example.Specific step is as follows:
(1) iris tissue culture transplantation of seedlings: 3-4 piece leaf is grown to " big capsicum " iris tissue-cultured seedling, 2-4 item root, root long is about
When 2-3cm, iris seedling is taken out from vial, clean the culture medium of root, impregnates " big capsicum " with Bravo solution
After 60 seconds, Bravo solution is that the Bravo pulvis for being 75% by active constituent content is diluted by quality for iris seedling root
It is obtained after 500 times, wraps up iris seedling root system with the sphagna sterilized, be placed in 1.5 cun of nutritive cubes；And with castanopsis fissa tree
The matrix that skin fragment, peat soil, decomposed husk and bagasse ash mix fills up nutritive cube, and the matrix is according to mass percent meter
It is 65 parts of castanopsis fissa bark pieces, 15 parts of peat soil, 15 parts of decomposed husk, 5 parts of bagasse ash；It is placed on after transplanting attached
There is culture in the greenhouse of sunshade net, cultivation temperature is 22-28 DEG C, natural lighting, intensity of illumination 4000-8000lux, illumination
Time is 12h/d, relative air humidity 70%-90%；
(2) apply fertilizer after transplanting: iris tissue-cultured seedling starts to apply fertilizer when having new root to grow, and applies the N:P:K=of 4000 times of dilution
The compound fertilizer of 10:26:15 applies primary for every 10 days；
(3) iris tissue-cultured seedling changes basin: when the root system of iris tissue-cultured seedling coils full in sphagna, in 3.5 cun of nutrition
Castanopsis fissa bark block about 3-4cm after the pad fermentation of alms bowl bottom is thick, is put into the iris tissue-cultured seedling after having shelled 1.5 cun of nutritive cubes, then
Nutritive cube is filled up with the matrix that castanopsis fissa bark block, peat soil and decomposed husk mix, the matrix is according to mass percent
Meter is 60 parts of castanopsis fissa bark pieces, 20 parts of peat soil, 20 parts of decomposed husk；Continue to be placed on sunshade after transplanting
Culture in the greenhouse of net, cultivation temperature are 23-30 DEG C, natural lighting, intensity of illumination 8000-10000lux, light application time
For 12h/d, relative air humidity 60%-80%；
(4) it applies fertilizer after changing basin: after iris tissue-cultured seedling changes basin 10 days, applying the N:P:K=26:12:12's of 1000 times of dilution
Compound fertilizer applies primary for every 10 days；
(5) promote Phalaenopsis plants suspend mode: when iris tissue-cultured seedling has 4 or more health into age leaf, with 30mg/L's
The acid solution that falls off pours Phalaenopsis plants root, which is 18-26 DEG C, intensity of illumination 6000-8000lux,
Light application time is 10h/d, relative air humidity 60%-80%, to promote Phalaenopsis plants suspend mode；
(6) the Flower induction phase:
1. Flower induction early period: in June, 2017 starts floral bud induction, and daily natural lighting is maintained at 15000-
25000lux, with incandescent lamp light filling when natural lighting deficiency, the natural lighting time is 12h/d；After natural lighting, continue pair
Iris carries out LED composite light source treatment with irradiation 4h, and feux rouges and blue light match R:B (light quantity ratio)=4:1；Night cultivation temperature
It is 16-18 DEG C, cultivation temperature on daytime is 25-28 DEG C, relative air humidity 70%-80%；And during this period every 10 days with dense
Spend the KH of the GA3 solution for being 400mg/L and 0.1%2PO4It is each primary that solution pours Phalaenopsis plants root；For suitable control culture
It is primary to spray blade face water daily for soil humidity；The stage cultivates 28 days；
2. the Flower induction later period: daily natural lighting is maintained at 15000-25000lux, with white when natural lighting deficiency
Vehement lamp light filling, natural lighting time are 12h/d；After natural lighting, continue to carry out at the irradiation of LED composite light source iris
4h is managed, wherein feux rouges and blue light match R:B (light quantity ratio)=4:1；Night cultivation temperature be 16-18 DEG C, daytime cultivation temperature
It is 25-28 DEG C, relative air humidity 70%-80%；During this period every 10 days with 2000 times of N:P:K=10:20:20 of dilution
Compound fertilizer's solution pour Phalaenopsis plants root；The stage cultivates 42 days；
(7) flower bud differentiation period: bennet it is long to 8-12cm when, be that 400mg/L 6-BA solution pours Phalaenopsis plants with concentration
Root；Daily natural lighting is maintained at 12000-15000lux, with incandescent lamp light filling, natural lighting time when natural light deficiency
It is 12 hours；After natural light, continue to carry out iris LED blue light illumination processing 3h；Cultivation temperature is 18-26 DEG C, empty
Gas relative humidity is 60%-70%；During this period every 10 days compound fertilizer's solution with 2000 times of dilution of N:P:K=10:25:15
Pour Phalaenopsis plants root；When bennet it is long to 15~20cm when, need that it is fixed, can remain upright
Growth；Meanwhile the phototropic face of petal is constantly adjusted, it can in uniform light；The stage cultivates 38 days.
On October 1st, 2017, the flower and unopened bud that statistics 200 basins " big capsicum " iris has opened are total
2296, average every plant of " big capsicum " iris has 11.5 (standard deviations 1.29).A number of wherein blooming has 1522, average
Every plant of " big capsicum " iris has 7.6 openings (standard deviation 0.95)；Unopened bud has 774, every plant average " big peppery
Green pepper " iris has 3.9 not open (standard deviations 0.62)；Flowering rate reaches 66.3%.The present invention is to " big capsicum " butterfly
The florescence of phalaenopsis is controllable, and " big capsicum " iris is bloomed simultaneously, and uniformity is high, and pattern is gorgeous, ornamental value with higher.
The above embodiments are only the preferred technical solution of the present invention, and are not construed as limitation of the invention, this hair
Bright protection scope should be with the technical solution of claim record, technical characteristic in the technical solution recorded including claim
Equivalents are protection scope.Equivalent replacement i.e. within this range is improved, also within protection scope of the present invention.
Claims (10)
1. a kind of iris tissue-cultured seedling florescence control method, which comprises the following steps:
1) transplanting iris tissue-cultured seedling is taken out from bottle, clean culture medium, then with Bravo solution impregnate, then with sterilize
The sphagna package iris root system crossed, is placed in nutritive cube and fills culture substrate, cultivated；Have newly to iris tissue-cultured seedling
Start to apply fertilizer when root long goes out；
2) basin is changed when the root system of iris tissue-cultured seedling coils full in sphagna, is changed the nutritive cube bigger than in step 1), is filled up training
Matrix is supported, continues to cultivate；Start to apply fertilizer after changing basin 10 days；
3) when suspend mode iris tissue-cultured seedling has 4 or more health into age leaf, with falling off, acid solution pours Phalaenopsis plants root
Portion, to promote Phalaenopsis plants suspend mode；
4) 120 days beginning floral bud inductions before the estimated list marketing of Flower induction phase, Flower induction early period be with concentration every 10 days
The GA3 solution of 400mg/L and 0.1% KH2PO4Solution pouring Phalaenopsis plants root is each primary, and spray blade face water is primary daily,
The stage cultivates 20-30 days；The Flower induction later period poured Phalaenopsis plants root, stage training with compound fertilizer's solution every 10 days
It supports 40-60 days；
5) flower bud differentiation period bennet it is long to 8-12cm when, be that 400 mg/L 6-BA solution pours Phalaenopsis plants root with concentration
Portion；Phalaenopsis plants root was poured with compound fertilizer's solution every 10 days during this period；When bennet it is long to 15~20 cm when, need pair
It is fixed, meanwhile, the phototropic face of petal is constantly adjusted, it can in uniform light；The stage cultivates 30-50 days.
2. according to the method described in claim 1, it is characterized by: iris tissue-cultured seedling has 3-4 piece leaf, 2-4 item in step 1)
It is taken out when root, root long 2-3cm, effective component content is used to impregnate 30- for the solution after 500 times of Bravo Dilution for powder of 75%
60s。
3. according to the method described in claim 1, it is characterized by: culture substrate is broken using castanopsis fissa bark in step 1)
Piece, peat soil, decomposed husk and bagasse ash are mixed by weight 60-65:10-20:10-20:5-8, the base in step 2
Matter is mixed using castanopsis fissa bark block, peat soil and 50-60 by weight parts: 20-25 part: 20-25 parts of decomposed husk.
4. according to the method described in claim 1, it is characterized by: being placed on the greenhouse with sunshade net when cultivating in step 1)
Interior culture, cultivation temperature are 22-28 DEG C, and natural lighting, intensity of illumination 4000-8000lux, light application time 12h/d are empty
Gas relative humidity is 70%-90%；When being cultivated in step 2, continues to be placed in the greenhouse with sunshade net and cultivate, cultivation temperature is
23-30 DEG C, natural lighting, intensity of illumination 8000-10000lux, light application time 12h/d, relative air humidity 60%-
80%；In step 3) when suspend mode, cultivation temperature is 18-26 DEG C, intensity of illumination 6000-8000lux, light application time 10h/d,
Relative air humidity is 60%-80%.
5. according to the method described in claim 1, it is characterized by: when applying fertilizer in step 1), apply the N:P:K of 4000 times of dilution=
The compound fertilizer of 10:26:15 applies primary for every 10 days；When applying fertilizer in step 2, answering for N:P:K=26:12:12 of 1000 times of dilution is applied
Hefei is applied primary for every 10 days.
6. according to the method described in claim 1, it is characterized by: daily natural lighting is maintained at 15000- in step 4)
25000lux, with incandescent lamp light filling when natural lighting deficiency, the natural lighting time is 12 h/d；After natural lighting, continue
LED composite light source treatment with irradiation 2-4h is carried out to iris；Night cultivation temperature is 16-18 DEG C, and cultivation temperature on daytime is 25-28
DEG C, relative air humidity 70%-80%.
7. according to the method described in claim 6, it is characterized by: the LED composite light source, is LED feux rouges and LED blue light
Two kinds of monochromatic light are combined into LED composite light source, and wherein the wavelength of LED feux rouges is 630nm；The wavelength of LED blue light is 450-
480nm, feux rouges and blue light light quantity match R:B=4:1.
8. according to the method described in claim 1, it is characterized by: daily natural lighting is maintained at 12000- in step 5)
15000lux, with incandescent lamp light filling when natural light deficiency, the natural lighting time is 12 hours；After natural light, continue to butterfly
Phalaenopsis carries out LED blue light illumination and handles 3-4h；Cultivation temperature is 18-26 DEG C, relative air humidity 60%-70%.
9. according to the method described in claim 8, it is characterized by: the LED blue light；The wavelength of its LED blue light is 450-
480nm。
10. according to the method described in claim 1, it is characterized by: the compound fertilizer solution in step 4) is N:P:K=10:20:
Compound fertilizer's solution that 20 dilution 2000 obtains again；Compound fertilizer solution in step 5) is that N:P:K=10:25:15 dilutes 2000 times
Obtained compound fertilizer's solution.
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| CN112868514A (en) * | 2021-01-11 | 2021-06-01 | 江苏中荷花卉股份有限公司 | Method for compounding cut flowers of butterfly orchid |
| CN113728891A (en) * | 2021-09-22 | 2021-12-03 | 厦门品诚源花卉科技有限公司 | Application of hypochlorous acid water in flower forcing in butterfly orchid cultivation process |
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| CN109769646A (en) * | 2019-03-31 | 2019-05-21 | 芜湖东源新农村开发股份有限公司 | Extend the breeding method at iris florescence |
| CN112042421A (en) * | 2020-09-08 | 2020-12-08 | 南通大学 | Method for promoting flowering of shrub willow |
| CN112042421B (en) * | 2020-09-08 | 2022-04-01 | 南通大学 | Method for promoting flowering of shrub willow |
| CN112868514A (en) * | 2021-01-11 | 2021-06-01 | 江苏中荷花卉股份有限公司 | Method for compounding cut flowers of butterfly orchid |
| CN112868514B (en) * | 2021-01-11 | 2023-11-03 | 江苏中荷花卉股份有限公司 | Method for repeating flowers produced by butterfly orchid cut peanuts |
| CN113854132A (en) * | 2021-09-07 | 2021-12-31 | 嵩明博源农业科技有限公司 | Cultivation method for promoting cymbidium hybridum seedlings to bloom in advance |
| CN113854132B (en) * | 2021-09-07 | 2023-03-14 | 嵩明博源农业科技有限公司 | Cultivation method for promoting early blooming of cymbidium hybridum seedlings |
| CN113728891A (en) * | 2021-09-22 | 2021-12-03 | 厦门品诚源花卉科技有限公司 | Application of hypochlorous acid water in flower forcing in butterfly orchid cultivation process |
| CN115500167A (en) * | 2022-10-20 | 2022-12-23 | 南京农业大学 | Method for inducing phalaenopsis flower stalks to germinate and bloom early by utilizing triiodobenzoic acid |
| CN116649213A (en) * | 2023-05-22 | 2023-08-29 | 漳州新镇宇生物科技有限公司 | Tissue culture method for butterfly orchid seedling reviving |
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Application publication date: 20190222 |