CN116649213A - Tissue culture method for butterfly orchid seedling reviving - Google Patents
Tissue culture method for butterfly orchid seedling reviving Download PDFInfo
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- CN116649213A CN116649213A CN202310575197.0A CN202310575197A CN116649213A CN 116649213 A CN116649213 A CN 116649213A CN 202310575197 A CN202310575197 A CN 202310575197A CN 116649213 A CN116649213 A CN 116649213A
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- butterfly orchid
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- 240000002292 Psychopsis papilio Species 0.000 title claims abstract description 74
- 238000012136 culture method Methods 0.000 title claims abstract description 21
- 239000001963 growth medium Substances 0.000 claims abstract description 10
- 238000000034 method Methods 0.000 claims abstract description 7
- 238000005286 illumination Methods 0.000 claims description 7
- 230000008901 benefit Effects 0.000 abstract description 3
- 238000005728 strengthening Methods 0.000 abstract 1
- 238000012360 testing method Methods 0.000 description 9
- 238000012258 culturing Methods 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 244000079298 Epidendrum obrienianum Species 0.000 description 1
- 206010052428 Wound Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 238000011056 performance test Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The application relates to the field of butterfly orchid tissue culture, and particularly discloses a tissue culture method for butterfly orchid seedling reviving, which at least comprises the following steps: the method comprises the steps of firstly, reversely cutting the butterfly orchid which is differentiated into 6-9 generations, wherein the reversely cutting step is carried out when the butterfly orchid is in a seedling strengthening stage, namely, cutting off part of stems and other parts of the butterfly orchid strong seedlings without damaging roots to obtain reversely cut seedlings, and secondly, placing the reversely cut butterfly orchid strong seedlings after reversely cutting into a culture medium for cultivation; the method can delay the cultivation period of the butterfly orchid so as to cope with the butterfly orchid market in different time periods and realize the maximization of the economic benefit of the butterfly orchid.
Description
Technical Field
The application relates to the field of butterfly orchid tissue culture, in particular to a butterfly orchid seedling-restoring tissue culture method.
Background
Butterfly orchid is a perennial herb, and the main breeding mode at present is carried out by tissue fluid cultivation and greenhouse cultivation.
Tissue fluid culture (tissue culture for short) is generally carried out by differentiating the root of butterfly orchid into multiple pieces, then tissue culturing, culturing in culture flask for 2-3 months, and transferring to greenhouse for potted culture.
However, since the butterfly orchid market is easily changed, and butterfly orchid grows all the time, the butterfly orchid will be backlogged in warehouse to cause huge loss when grown to a certain extent, and if the butterfly orchid is differentiated, the risk of butterfly orchid mutation will be increased, so a butterfly orchid seedling-recovering technology needs to be proposed to solve the above problems.
Disclosure of Invention
In order to delay the cultivation period of the butterfly orchid and realize the maximization of the economic benefit of the butterfly orchid, the application provides two tissue culture methods for the butterfly orchid seedling recovery, which adopts the following technical scheme:
a tissue culture method for butterfly orchid seedling, comprising the following steps;
the method comprises the steps of firstly, reversely cutting the butterfly orchid which is differentiated into 6-9 generations, wherein the reversely cutting step is carried out when the butterfly orchid is in a strong seedling stage, and the reversely cutting step is to cut off part of stems and other parts of the butterfly orchid strong seedling without damaging roots, so as to obtain reversely cut seedlings;
step two, placing the reversely cut seedlings after reversely cutting into a culture medium for cultivation;
and step three, repeating the step one and the step two at least zero times.
A tissue culture method for butterfly orchid seedling, comprising the following steps;
the method comprises the steps of firstly, reversely cutting the butterfly orchid which is differentiated into 6-8 generations, wherein the reversely cutting step is carried out when the butterfly orchid is in a strong seedling stage, and the reversely cutting step is to cut off part of stems and other parts of the butterfly orchid strong seedling without damaging roots, so as to obtain reversely cut seedlings;
step two, placing the reversely cut seedlings after reversely cutting into a culture medium for cultivation;
step three, repeating the step one and the step two at least zero times;
cutting the strong butterfly orchid seedlings obtained in the second step, and placing the cut strong butterfly orchid seedlings in a culture medium for cultivation;
and fifth, repeating the first step to the fourth step.
Preferably, the back cutting step is to cut off part of the stem and other parts of the strong butterfly orchid seedling without damaging the root, and the length of the stem is kept at 1/10-1/5.
Preferably, the reverse cutting seedling is irradiated for more than half a month through the illumination intensity of 1000-2000 lux between the second step and the third step.
In summary, the application has the following beneficial effects:
according to the application, the root is not differentiated in a reverse cutting mode, the original offspring stage of the butterfly orchid is reserved, the risk of increasing the factor generation stage number and increasing the variation rate is effectively reduced, and the actual experiment shows that the time for growing strong seedlings after each reverse cutting can be more than half a year, which is equivalent to that for reviving seedlings after each reverse cutting for more than half a year, the butterfly orchid can be maintained for one year after being reversely cut for two times, so that the butterfly orchid can be piled in a warehouse first, sold in the coming year or in the market after selling price, and the benefit is maximized.
The application preferably irradiates the reverse cut seedlings for more than half a month with the illumination intensity of 1000-2000 lux, has lower light intensity, can reduce the stimulation to the reverse cut wounds and improve the survival rate or the stability of plants.
Description of the embodiments
The present application will be described in further detail with reference to examples.
Example 1: a tissue culture method for butterfly orchid seedling, comprising the following steps;
the method comprises the steps of firstly, reversely cutting the butterfly orchid which is differentiated into 7 generations, wherein the reversely cutting step is carried out when the butterfly orchid is in a strong seedling stage, and the reversely cutting step is to cut off part of stems and other parts of the butterfly orchid strong seedlings under the condition that roots are not damaged, so as to obtain reversely cut seedlings;
step two, placing the reversely cut seedlings after reversely cutting into a culture medium for cultivation;
step three, repeating step one and step two at least zero times, preferably zero times in this embodiment, and may be multiple times in other embodiments:
the butterfly orchid which is reversely cut in the first step is preferably butterfly orchid which has developed to strong seedlings, which can prolong the seedling-recovering time, and of course, butterfly orchid which has not developed to strong seedlings after differentiation can also be reversely cut. And step two, when the reversely cut seedlings are placed in a culture medium for cultivation, the tissue culture liquid can be cultivated by adopting the tissue culture liquid after cutting in the prior art, and the illumination intensity can also be carried out by referring to the tissue culture light intensity after cutting.
The time for growing strong seedlings after each back cutting in the second step can be more than half a year, which is equivalent to more than half a year of seedling reviving.
Example 2: a tissue culture method for butterfly orchid seedlings is different from example 1 in that:
step three, repeating step one and step two at least zero times, preferably zero times in this embodiment, and may be repeated multiple times in other embodiments;
cutting the strong butterfly orchid seedlings obtained in the second step, and placing the cut strong butterfly orchid seedlings in a culture medium for cultivation;
and fifth, repeating the first step to the fourth step.
In this embodiment, the back-cutting and the splitting can be alternately performed, and as the offspring has stronger cell activity, the back-cutting is performed again after the offspring stage number is increased, so that various problems caused by the aging of the back-cut seedling cells caused by repeated continuous back-cutting can be effectively solved.
Example 3: the tissue culture method for butterfly orchid seedlings is different from example 1 or 2 in that: the reverse cutting step is to cut off part of stem and other parts of strong butterfly orchid seedling without damaging root, and the length of stem is kept 1/10.
Example 4: the tissue culture method for butterfly orchid seedlings is different from example 1 or 2 in that: the reverse cutting step is to cut off part of stem and other parts of strong butterfly orchid seedling without damaging root, and the length of stem is kept 1/5.
Example 5: the tissue culture method for butterfly orchid seedlings is different from example 1 or 2 in that: and (3) between the second step and the third step, reversely cutting the seedlings by irradiation of the illumination intensity of 1000lux for more than half a month.
Example 6: a tissue culture method for butterfly orchid seedlings is different from example 5 in that: the illumination intensity was 1500lux.
Example 7: a tissue culture method for butterfly orchid seedlings is different from example 5 in that: the illumination intensity was 2000lux.
Example 8: the tissue culture method for butterfly orchid seedlings is different from example 1 or 2 in that: in the first step, the butterfly orchid of the differentiation 6 generation is reversely cut.
Example 9: the tissue culture method for butterfly orchid seedlings is different from example 1 or 2 in that: in the first step, the butterfly orchid of the differentiated 8 generations is reversely cut.
Example 10: a tissue culture method for butterfly orchid seedlings is different from example 1 in that: in the first step, the butterfly orchid of 9 generations is differentiated for reverse cutting.
Performance test:
test one seedling time period test:
test sample: the butterfly orchid seedlings obtained in examples 1 to 10 were used as test samples 1 to 10.
The test method comprises the following steps: the test samples 1 to 10 thus obtained were cultured in the same medium, and the time period until the growth to strong seedlings was observed. The largest 5 values and the smallest 5 values of each group were discarded, and the remainder averaged.
Test results: it was found that test samples 1-10 all required at least half a year to develop strong seedlings and that test sample 2 was extended by about 3 months relative to test sample 1 as a result of the addition of one step.
The present embodiment is only for explanation of the present application and is not to be construed as limiting the present application, and modifications to the present embodiment, which may not creatively contribute to the present application as required by those skilled in the art after reading the present specification, are all protected by patent laws within the scope of claims of the present application.
Claims (4)
1. The tissue culture method for the butterfly orchid seedlings is characterized by comprising the following steps of:
the method comprises the steps of firstly, reversely cutting the butterfly orchid which is differentiated into 6-9 generations, wherein the reversely cutting step is carried out when the butterfly orchid is in a strong seedling stage, and the reversely cutting step is to cut off part of stems and other parts of the butterfly orchid strong seedling without damaging roots, so as to obtain reversely cut seedlings;
step two, placing the reversely cut seedlings after reversely cutting into a culture medium for cultivation;
and step three, repeating the step one and the step two at least zero times.
2. The tissue culture method for the butterfly orchid seedlings is characterized by comprising the following steps of;
the method comprises the steps of firstly, reversely cutting the butterfly orchid which is differentiated into 6-8 generations, wherein the reversely cutting step is carried out when the butterfly orchid is in a strong seedling stage, and the reversely cutting step is to cut off part of stems and other parts of the butterfly orchid strong seedling without damaging roots, so as to obtain reversely cut seedlings;
step two, placing the reversely cut seedlings after reversely cutting into a culture medium for cultivation;
step three, repeating the step one and the step two at least zero times;
cutting the strong butterfly orchid seedlings obtained in the second step, and placing the cut strong butterfly orchid seedlings in a culture medium for cultivation;
and fifth, repeating the first step to the fourth step.
3. The tissue culture method for the butterfly orchid seedlings according to claim 1 or 2, characterized in that: the reverse cutting step is to cut off part of stem and other parts of strong butterfly orchid seedling without damaging root and to maintain the length of stem 1/10-1/5.
4. The tissue culture method for the butterfly orchid seedlings according to claim 1 or 2, characterized in that the reverse cutting seedlings are irradiated for more than half a month by the illumination intensity of 1000-2000 lux between the second step and the third step.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310575197.0A CN116649213A (en) | 2023-05-22 | 2023-05-22 | Tissue culture method for butterfly orchid seedling reviving |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310575197.0A CN116649213A (en) | 2023-05-22 | 2023-05-22 | Tissue culture method for butterfly orchid seedling reviving |
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| CN116649213A true CN116649213A (en) | 2023-08-29 |
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| CN202310575197.0A Pending CN116649213A (en) | 2023-05-22 | 2023-05-22 | Tissue culture method for butterfly orchid seedling reviving |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101416588A (en) * | 2008-11-14 | 2009-04-29 | 广东省农业科学院土壤肥料研究所 | Cut-flower cultivation device and method on protected ground |
| WO2018161921A1 (en) * | 2017-03-08 | 2018-09-13 | 南京农业大学 | Method for epigenetically manipulating plant phenotypic plasticity |
| CN109362545A (en) * | 2018-10-11 | 2019-02-22 | 广西生态工程职业技术学院 | A kind of iris tissue-cultured seedling florescence control method |
| CN110432155A (en) * | 2019-09-19 | 2019-11-12 | 漳州新镇宇生物科技有限公司 | A kind of iris sugar free tissue culture method |
-
2023
- 2023-05-22 CN CN202310575197.0A patent/CN116649213A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101416588A (en) * | 2008-11-14 | 2009-04-29 | 广东省农业科学院土壤肥料研究所 | Cut-flower cultivation device and method on protected ground |
| WO2018161921A1 (en) * | 2017-03-08 | 2018-09-13 | 南京农业大学 | Method for epigenetically manipulating plant phenotypic plasticity |
| CN109362545A (en) * | 2018-10-11 | 2019-02-22 | 广西生态工程职业技术学院 | A kind of iris tissue-cultured seedling florescence control method |
| CN110432155A (en) * | 2019-09-19 | 2019-11-12 | 漳州新镇宇生物科技有限公司 | A kind of iris sugar free tissue culture method |
Non-Patent Citations (2)
| Title |
|---|
| 她品: "《健康花草种植一点通》", 31 March 2014, 福建科学技术出版社, pages: 188 - 189 * |
| 张彩玲;: "蝴蝶兰花梗组织培养与快速繁殖的研究", 中国林副特产, no. 05, 15 October 2010 (2010-10-15), pages 42 - 44 * |
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