CN113016481B - Application of mixed light in promoting growth of cuttage plants and cultivation method - Google Patents
Application of mixed light in promoting growth of cuttage plants and cultivation method Download PDFInfo
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- CN113016481B CN113016481B CN202110256756.2A CN202110256756A CN113016481B CN 113016481 B CN113016481 B CN 113016481B CN 202110256756 A CN202110256756 A CN 202110256756A CN 113016481 B CN113016481 B CN 113016481B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/04—Electric or magnetic or acoustic treatment of plants for promoting growth
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/14—Measures for saving energy, e.g. in green houses
Abstract
The invention belongs to the field of plant cutting, and particularly relates to an application of mixed light in promoting the growth of cutting plants and a cultivation method. The invention provides an application of mixed light in promoting the growth of cutting plants. The invention provides an application of effectively utilizing mixed light to promote the growth of cutting plants and improve the quality of cutting plant seedlings by limiting the proportion and the types of the mixed light. The mixed light treatment provided by the invention obviously improves the data of camphor tree cutting seedlings in the aspects of biomass, chlorophyll content, growth state, metabolism and the like.
Description
Technical Field
The invention belongs to the field of plant cutting, and particularly relates to an application of mixed light in promoting the growth of cutting plants and a cultivation method.
Background
The quality of seedlings determines the growth and yield of the forest tree, and the industrialization of seedling cultivation has become an important means for producing high-quality seedlings. The plant factory is a high-precision environment control type efficient facility agriculture mode, the internal environment conditions such as illumination, temperature, humidity, nutrient solution and the like can be automatically regulated and controlled, the plant factory is little influenced by natural conditions, the production planning performance is strong, the production period is short, the automation degree is high, the seedling quality, the quantity and the land utilization rate can be remarkably improved, and the plant factory is a highly specialized and modern seedling production mode developed after greenhouse seedling cultivation. The light quality is one of important environmental impact factors in plant factory seedling cultivation, is one of core technologies of factory seedling cultivation, and can regulate each process of plant growth and development.
The camphor tree (Cinnamomum camphora) belongs to the genus camphor of the family Lauraceae, is an important economic tree species and an afforestation tree species in China, and is continuously developed in application to afforestation and perfume essential oil in recent years, so that the economic value of improved varieties of camphor trees is gradually improved, and the camphor tree industry is continuously expanded. Seedling raising is an important content in camphor tree industry. Illumination is one of important environmental influence factors in seedling culture, but the influence of illumination on camphor tree seedling culture has not yet been clearly studied.
Disclosure of Invention
In order to solve the problems, the invention provides an application of mixed light in promoting the growth of cutting plants and a cultivation method. In the application provided by the invention, the mixed light with specific wavelength can effectively promote the growth of plant cutting seedlings and improve the quality of the cutting seedlings, and provides basic data and technical support for promoting the fine variety process of camphor trees.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides an application of mixed light in promoting the growth of cutting plants; the mixed light includes a first mixed light and a second mixed light;
the first mixed light comprises red light and blue light, and the effective light quantum flux density ratio of the red light and the blue light in the first mixed light is 8:1; the second mixed light comprises red light, blue light and ultraviolet light, and the effective light quantum flow density ratio of the red light, the blue light and the ultraviolet light in the second mixed light is 20:5:1.
The invention also provides application of the mixed light in promoting the plant height, stem thickness, individual plant leaf number and biomass of the cuttage plant; the mixed light comprises a first mixed light and/or a second mixed light;
the first mixed light comprises red light and blue light, and the effective light quantum flux density ratio of the red light and the blue light in the first mixed light is 8:1; the second mixed light comprises red light, blue light and ultraviolet light, and the effective light quantum flow density ratio of the red light, the blue light and the ultraviolet light in the second mixed light is 20:5:1.
The invention also provides application of the mixed light in promoting photosynthesis of cutting plants; the mixed light comprises a first mixed light and/or a second mixed light;
the first mixed light comprises red light and blue light, and the effective light quantum flux density ratio of the red light and the blue light in the first mixed light is 8:1; the second mixed light comprises red light, blue light and ultraviolet light, and the effective light quantum flow density ratio of the red light, the blue light and the ultraviolet light in the second mixed light is 20:5:1.
The invention also provides application of the mixed light in promoting the chloroplast pigment content of the cuttage plant; the mixed light comprises a first mixed light and/or a second mixed light;
the first mixed light comprises red light and blue light, and the effective light quantum flux density ratio of the red light and the blue light in the first mixed light is 8:1; the second mixed light comprises red light, blue light and ultraviolet light, and the effective light quantum flow density ratio of the red light, the blue light and the ultraviolet light in the second mixed light is 20:5:1.
Preferably, the light intensity of the mixed light is 80 to the whole100μmol/·m -2 ·s -1 。
Preferably, the time of illumination of the mixed light is 16h/d.
The invention also provides a cultivation method for promoting the growth of the cuttage plants, which comprises the following steps: the above mixed light treatment is given to the cutting slips every day from the beginning to the end of the day of insertion of the cutting slips into the cutting medium.
Preferably, the cultivation temperature in the cultivation method is 18-22 ℃.
Preferably, the plant comprises camphor tree.
The invention provides an application of mixed light in promoting the growth of cutting plants. The invention provides an application of effectively utilizing mixed light to promote the growth of cutting plants and improve the quality of cutting plant seedlings by limiting the wavelength and the variety of the mixed light. From the data of the examples, the biomass, chlorophyll content, growth state, metabolism and other data of camphor tree cutting seedlings are obviously improved after the mixed light treatment provided by the invention.
Drawings
FIG. 1 is a graph showing the effect of different lights on the plant height variation of camphor tree cutting container seedlings;
FIG. 2 shows the effect of different lights on the variation of stem thickness of camphor tree cutting container seedlings;
FIG. 3 shows the effect of different lights on the variation of leaf number of camphor tree cutting container seedlings.
Detailed Description
The invention provides an application of mixed light in promoting the growth of cutting plants; the mixed light includes a first mixed light and a second mixed light;
the first mixed light comprises red light and blue light, and the effective light quantum flux density ratio of the red light and the blue light in the first mixed light is 8:1; the second mixed light comprises red light, blue light and ultraviolet light, and the effective light quantum flow density ratio of the red light, the blue light and the ultraviolet light in the second mixed light is 20:5:1.
In the invention, the wavelength of the red light is preferably 650-670 nm; the wavelength of the blue light is preferably 440-460 nm, and the wavelength of the ultraviolet light is preferably 380-400 nm; the mixingThe light intensity is preferably 80 to 100. Mu. Mol/. Mu.m -2 ·s -1 The method comprises the steps of carrying out a first treatment on the surface of the In the application, the time of the mixed light illumination is preferably 16h/d; the plant preferably comprises camphor tree.
The application provided by the invention can effectively promote the growth of the cutting container seedlings, promote the light and parameters of the cutting container seedlings, accelerate the metabolism of the cutting container seedlings and improve the chloroplast pigment content of the cutting container seedlings.
The invention also provides application of the mixed light in promoting the plant height, stem thickness, individual plant leaf number and biomass of cutting plants. The mixed light is as indicated above and is not repeated here. The application provided by the invention can improve the plant height, the stem thickness, the number of single plant leaves and the biomass of camphor tree cutting container seedlings.
The invention also provides application of the mixed light in promoting metabolism of cutting plants. The mixed light is as indicated above and is not repeated here.
The invention also provides application of the mixed light in promoting photosynthesis of cutting plants. The mixed light is as indicated above and is not repeated here. The application provided by the invention can effectively improve the net photosynthetic rate and the pore conductance of camphor tree cutting container seedlings.
The invention also provides application of the mixed light in promoting the chloroplast pigment content of the cuttage plants. The mixed light is as indicated above and is not repeated here. The application provided by the invention can improve the chlorophyll a content, the chlorophyll b content and the carotenoid content of camphor tree cutting container seedlings.
The invention also provides a cultivation method for promoting the growth of the cuttage plants, which comprises the following steps: the above mixed light treatment is given to the cutting slips every day from the beginning to the end of the day of insertion of the cutting slips into the cutting medium. In the present invention, the light intensity of the mixed light is preferably 80. Mu. Mol/. M -2 ·s -1 The method comprises the steps of carrying out a first treatment on the surface of the The illumination time of the mixed light is preferably 16h/d.
In the present invention, the cultivation method preferably takes place in a plant factory;
the cultivation method preferably comprises the following steps: inserting plant branches into a cuttage matrix for rooting, and transplanting the plant branches into a nutrition pot after the plant branches root; the temperature in the cultivation process is preferably 18-22 ℃; the humidity in the rooting process is preferably 70-80%, and the humidity after rooting is preferably 60-70%; the cutting matrix is preferably peat: perlite: vermiculite = 1:1:1, the matrix composition in the nutritional bowl preferably being peat: perlite: vermiculite = 2:1:1.
The cultivation method provided by the invention has the advantages of regulating and controlling plant growth and improving the quality and quality of cutting seedlings, and can also shorten the seedling raising period.
For further explanation of the present invention, the application of the mixed light provided by the present invention to promotion of growth of cutting plants and cultivation method will be described in detail with reference to the accompanying drawings and examples, which should not be construed as limiting the scope of the present invention.
Example 1
Setting light quality treatment: red light (R) =8:1, the wavelength of the red light is 660±10nm, and the wavelength of the blue light is 450±10nm.
The test is carried out in a fully artificial light plant factory, and various conditions in the plant factory during cutting are set as follows: the temperature is 20+/-2 ℃ and the humidity is 70-80%; cutting with a three-dimensional cultivation frame, wherein the light source of each layer adopts LED lamp tubes provided by medium-energy-saving crystal and illumination limited company, the power of the lamp tubes is 18W, 3 lamp tubes are placed on each layer (the area of each layer is 1.2mx0.4m, the distance between the lamp tubes and plants is about 20cm, the lamp tubes are placed in parallel with the length direction of each layer, if no special description exists, the illumination is uniform), the light quality is red light (R) which is blue light (B) =8:1, the illumination time is 16h/d, and the light intensity is 80 mu mol/. M -2 ·s -1 . The illumination period is as follows: and (5) starting from the current day of inserting the cutting slips into the cutting matrix to the completion of rooting the cutting slips.
Cutting in a plant factory room in the 11 th and 15 th 2018 days, wherein the cutting matrix is peat: perlite: vermiculite=1:1:1, adopting terminal bud cutting, taking cutting slips from outdoor nursery camphor tree, cutting about 10cm, leaving 2-3 leaves on branches, diluting the lower part of the cutting slips by water with the mass percent of 50% of carbendazim with 1000 times of N- (2-benzimidazolyl) -methyl carbamate, soaking the whole cutting slips for 0.5-1 h for disinfection, soaking the part of the cutting slips far from the top by using indolebutyric acid (IBA) with the concentration of 3000mg/L for 1/4-1/3,0.5-1 min, and then inserting 1/3 of the part of the cutting slips far from the top into a cutting matrix.
Rooting of cutting shoot (root length)>1cm, number of>2) After the completion, transplanting the seedlings into a nutrition pot (12 cm multiplied by 13 cm) for 1 month and 5 days, and obtaining the cutting container seedlings. The matrix in the nutrition pot comprises peat: perlite: vermiculite=2:1:1, periodically (100 mL/2 weeks) pouring 1/2Hogland nutrient solution, continuously growing the cuttage container seedlings on a plant factory three-dimensional cultivation frame, keeping the humidity of the plant factory at 60% -70%, keeping the temperature at 20+/-2 ℃, keeping the illumination time at 16h/d, and keeping the light intensity at 80 mu mol/. M -2 ·s -1 The light quality is R, B=8:1, and the cutting container seedlings are investigated for growth once on 1 month, 5 days, 2 months, 15 days and 3 months, 18 days.
When the growth state is investigated, the plant height is measured by adopting a ruler, and the plant height is the height from the stem soil intersection position of the plant to the highest position of the plant; measuring the stem thickness by a vernier caliper, wherein the stem thickness is the diameter of the position 1cm upwards at the plant stem soil intersection; the branch number refers to the number of lateral branches with the length of the outer main stem being more than 3 cm; the fresh weight of the overground part and the fresh weight of the underground part are weighed by a balance, and the dry weight of the overground part and the dry weight of the underground part are dried by an oven at 105 ℃ and then are weighed after being dried at 70 ℃.
And (3) measuring each photosynthesis parameter of camphor tree leaves by adopting a Li-6400XT photosynthetic apparatus for 18 days, arbitrarily selecting 3 plants, and measuring a third leaf at the upper part of a branch of the plants at 9 to 11 am, wherein the measurement indexes comprise: net photosynthetic rate (Pn), stomatal conductance (Cond), intercellular CO 2 Concentration (Ci) and transpiration rate (Tr); the chlorophyll content of camphor tree is determined by spectrophotometry, each time randomly mixing 2-3 leaves on main stem, and each treatment is repeated for 3 times.
And (3) carrying out data analysis of variance by adopting an SPSS 20.0 system, and carrying out multiple comparison by using a new complex polar difference method.
Example 2
The cutting treatment is as shown in example 1, and the light quality treatment is red light (R), blue light (B), ultraviolet light A (UVA) =20:5:1, the wavelength of the red light is 660+ -10 nm, the wavelength of the blue light is 450+ -10 nm, and the wavelength of the ultraviolet light UV-A is 390+ -10 nm after the cutting rooting is completed.
Other treatments were as in example 1.
Comparative example 1
Setting a natural light group, carrying out cutting treatment, moving the cutting seedling into a greenhouse after cutting rooting is completed as shown in the embodiment 1, and enabling the cutting seedling to continue to grow under the illumination of natural light (without adding any artificial light), wherein the greenhouse is free of temperature and humidity control equipment.
Other treatments were as in example 1.
Comparative example 2
The light quality treatment was set, and the light quality treatment after the rooting of the cutting was completed was blue light (B) having a wavelength of 450.+ -.10 nm as shown in example 1.
Other treatments were as in example 1.
Comparative example 3
Setting light quality treatment: the cutting treatment is as shown in example 1, the light quality treatment is red light (R) after the cutting rooting is completed, and the wavelength of the red light is 660+/-10 nm.
Other treatments were as in example 1.
Application example 1
Influence of different lights on growth of cutting container seedlings
The change of the plant height, stem thickness, leaf number and other data of the cutting container seedlings is described as shown in fig. 1, fig. 2, fig. 3, table 1, table 2, table 3 and table 4. Wherein figure 1 shows the effect of different lights on the plant height variation of camphor tree cutting container seedlings; FIG. 2 shows the effect of different lights on the variation of stem thickness of camphor tree cutting container seedlings; FIG. 3 shows the effect of different lights on the variation of leaf number of camphor tree cutting container seedlings.
TABLE 1 influence of different lights on plant height (cm) variation of camphor tree cutting container seedlings
Note that: the different lower case letters after the same column of data in the table indicate significant differences (P < 0.05), as follows. (n >3, mean+ -SD)
TABLE 2 influence of different lights on variation in stem thickness (mm) of camphor tree cutting container seedlings
TABLE 3 influence of different lights on the variation of leaf number of container seedlings for cutting camphor tree
TABLE 4 influence of different lights on growth of camphor tree cutting container seedlings (measured on day 18 of 3 months)
As is clear from FIGS. 1 to 3 and tables 1 to 3, there was no significant difference in plant height, stem thickness and leaf number under each light quality at 1 month and 5 days for camphor tree container cutting seedlings.
The cutting of the example did not show a significant difference in stem thickness from the comparative example at day 2 for 15, but the plant height of example 2 was significantly higher than that of comparative example 1 and comparative example 2, and the number of leaves of example 2 was significantly greater than that of comparative example.
By day 3 month 18, the plant height was significantly increased in example 2 compared to comparative example 1 and comparative example 2, although there was no significant difference in plant height compared to comparative example 3;
although there is no significant difference between example 2 and comparative example 1, example 2 significantly increases stem thickness compared to comparative example 2;
the most number of blades of example 1, which is significantly different from each comparative example, is higher than that of the comparative example;
as can be seen from table 4, at day 3 and 18, the number of branches of example 2 was significantly higher than that of comparative example 2, and the number of branches of the example group as a whole was higher than that of comparative example;
wherein the fresh weight of the aerial parts of the cutting seedlings of the example 1 group is highest and is significantly higher than that of the comparative examples 1 and 2;
the fresh weight of the underground part of the cutting seedlings in example 2 is highest and is significantly higher than that of comparative examples 1 and 2;
the dry weight of the aerial parts of the cutting seedlings in example 1 was highest, significantly higher than that of comparative examples 1 and 2;
the dry weight of the underground part of the cutting in example 2 was highest, significantly higher than that of comparative example 2.
In conclusion, the mixed light provided by the invention can effectively promote the growth of the cutting container seedlings.
Application example 2
Influence of different light sources on photosynthetic parameters of cutting container seedlings
The measurement of photosynthetic parameters was performed on camphor tree container seedlings by using a Li6400XT photosynthetic apparatus, and the results are shown in Table 5.
TABLE 5 influence of different light sources on photosynthetic parameters of camphor tree
As can be seen from table 5, the mixed light provided in example 2 of the present invention can significantly improve the net photosynthetic rate compared to comparative examples 1 and 3.
Influence of light on chloroplast pigment of cutting container seedlings
The different lights showed significant differences in the chloroplast pigment content of camphor tree as shown in table 6.
TABLE 6 influence of different lights on the chloroplast pigment content of camphor tree leaves
As can be seen from table 6, the chlorophyll a content under the treatment of example 2 was highest, significantly higher than that of comparative examples 1 and 3;
the chlorophyll b content was highest under the treatment of example 2, significantly higher than that of comparative example 3;
for chlorophyll a/chlorophyll b, there was no significant difference between the other treatments except comparative example 1;
in the detection of carotenoid content, the carotenoid content was highest in the treatment of example 2, which was significantly higher than that of comparative example 3.
In summary, the two mixed lights provided by the embodiment of the invention have the technical effect of influencing the chloroplast pigment content of the cutting container seedlings.
In the test, the chloroplast pigment content of the camphor tree leaves under red light irradiation is obviously lower than that of other treatments, the growth amount of camphor tree cuttage container seedlings under natural light and blue light is reduced to a certain extent, and the biomass accumulation of the seedlings under mixed light treatment provided by the embodiment of the invention is highest, so that the mixed light provided by the embodiment of the invention can promote the formation of chlorophyll of the camphor tree leaves.
The light influences the physiological processes of plant stomata opening, chloroplast development, photosynthetic reaction and the like through photoreceptors such as photosensitive pigments, cryptomelane, phototropism and the like, and regulates and controls the morphogenesis of plants. From the above experiments, it is known that although individual items perform better under natural light conditions, each item performs generally poorly; the photosynthesis capacity of plants is limited by the irradiation of monochromatic light, and the growth is inhibited; the mixed light provided by the invention can effectively promote plant growth, improve chlorophyll synthesis and regulate the physiological process of plants.
In conclusion, the mixed light irradiation provided by the invention is adopted to carry out cutting seedling, so that the seedling period of camphor trees can be effectively shortened.
While the invention has been described in terms of preferred embodiments, it is not intended to be limited thereto, but rather to enable any person skilled in the art to make various changes and modifications without departing from the spirit and scope of the present invention, which is therefore to be limited only by the appended claims.
Claims (7)
1. The application of the mixed light in promoting the plant height, stem thickness, individual plant leaf number and biomass of the cutting camphor tree; the mixed light includes a first mixed light or a second mixed light;
the first mixed light comprises red light and blue light, and the effective light quantum flux density ratio of the red light and the blue light in the first mixed light is 8:1; the second mixed light comprises red light, blue light and ultraviolet light, and the effective light quantum flow density ratio of the red light, the blue light and the ultraviolet light in the second mixed light is 20:5:1.
2. Application of mixed light in promoting photosynthesis of cutting camphor tree; the mixed light includes a first mixed light or a second mixed light;
the first mixed light comprises red light and blue light, and the effective light quantum flux density ratio of the red light and the blue light in the first mixed light is 8:1; the second mixed light comprises red light, blue light and ultraviolet light, and the effective light quantum flow density ratio of the red light, the blue light and the ultraviolet light in the second mixed light is 20:5:1.
3. Application of mixed light in promoting the chloroplast pigment content of cutting camphor tree; the mixed light includes a first mixed light or a second mixed light;
the first mixed light comprises red light and blue light, and the effective light quantum flux density ratio of the red light and the blue light in the first mixed light is 8:1; the second mixed light comprises red light, blue light and ultraviolet light, and the effective light quantum flow density ratio of the red light, the blue light and the ultraviolet light in the second mixed light is 20:5:1.
4. The use according to any one of claims 1 to 3, wherein the light intensity of the mixed light is 80 to 100 μmol.m -2 ·s -1 。
5. A use according to any one of claims 1 to 3, wherein the time of illumination with the mixed light is 16h/d.
6. A cultivation method for promoting growth of cutting camphor tree comprises the following steps: cultivating from the day of inserting the cutting slips into the cutting matrix to the end, and carrying out treatment of mixed light of the cutting slips every day; the mixed light includes a first mixed light or a second mixed light;
the first mixed light comprises red light and blue light, and the effective light quantum flux density ratio of the red light and the blue light in the first mixed light is 8:1; the second mixed light comprises red light, blue light and ultraviolet light, and the effective light quantum flow density ratio of the red light, the blue light and the ultraviolet light in the second mixed light is 20:5:1.
7. The cultivation method according to claim 6, wherein the cultivation temperature in the cultivation method is 18℃to 22 ℃.
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