CN113099878A

CN113099878A - Method for promoting anthocyanin content accumulation of plant factory flowers

Info

Publication number: CN113099878A
Application number: CN202110312542.2A
Authority: CN
Inventors: 李阳; 王婷婷; 丁爽爽
Original assignee: Fujian Sanan Sino Science Photobiotech Co Ltd
Current assignee: Fujian Sanan Sino Science Photobiotech Co Ltd; Fujian Province Sino Science Biological Co Ltd
Priority date: 2021-03-24
Filing date: 2021-03-24
Publication date: 2021-07-13

Abstract

The invention discloses a method for promoting the anthocyanin content accumulation of plant plants, which adopts a total artificial light source with the wavelength range of 380-780nm, wherein the proportion of the light quantum number of 380-499nm to the total light quantum number of the light source is 5-12%, the proportion of the light quantum number of 500-599nm to the total light quantum number of the light source is 12-18%, and the proportion of the light quantum number of 600-780nm to the total light quantum number of the light source is 65-77%. The invention is obtained by carrying out a large amount of creative tests on the basis that testers find that LED red lights with different wavelengths play a key role in regulating and controlling the flower color of herbaceous flowers during flower planting in plant factories, and the commercial value of the flowers can be realized by regulating and controlling the anthocyanin content to influence the flower color or extracting the anthocyanin for other industries such as food coloring, dyes, medicines, cosmetics and the like.

Description

Method for promoting anthocyanin content accumulation of plant factory flowers

Technical Field

The invention belongs to the technical field of plant cultivation, and particularly relates to a method for promoting the anthocyanin content accumulation of plant plants.

Background

The pigment which is the most important pigment for creating the color of flowers is called anthocyanin, and is distributed in vacuoles of cells to control the color changes of pink, red, purple, blue and the like of the flowers. In daily life, the quality grading of flowers is different according to different flower varieties. Some flowers are objectively graded in quality according to the size of the flower diameter, the length of the flower stalk, the weight of a single branch and the like, and some flowers are subjectively graded in quality according to the shape, color, smell, cleanliness, luster and the like of the flowers. At present, the quality of flowers is improved mainly by 4 environmental factors such as water, fertilizer, mild light and the like. The influence of light on the growth and development of plants mainly passes through the light quality, the light intensity and the light period, and a large number of documents show that the light has an important regulation and control function on the germination of plant seeds, the growth of rhizomes, flowering and fruiting and the like, but the research on the influence of the light on the morphology and the color of flowers is almost in a blank stage. Only the color regulation and control research mainly focuses on light supplement, or LED light quality treatment is carried out in the flower bud differentiation stage. For example, the LED photoperiod treatment is carried out on azolla in the bud differentiation stage of the phalaenopsis, and the 7R1B (R: B ═ 7) is found to be beneficial to the development of phalaenopsis flowers and shows that the anthocyanin content is increased. However, the combination ratio of red light and blue light is not a single factor for regulating the color of flowers in the actual production of the flowers.

Whether the commercial value of flowers is realized by regulating and controlling the anthocyanin content to influence the flower color, or the anthocyanin is extracted to be used in other industries such as food coloring, dyes, medicines, cosmetics and the like, the improvement of the anthocyanin content in the flowers in plant factories is very important.

Disclosure of Invention

Aiming at the defects of the prior art, the invention aims to provide a light environment regulation method for promoting the anthocyanin content accumulation of plant plants.

The invention adopts the specific technical scheme that:

a method for promoting the anthocyanin content accumulation of plant plants and plants adopts a total artificial light source with the wavelength range of 380-780nm, wherein the proportion of the light quantum number of 380-499nm to the total light quantum number of the light source is 5-12%, the proportion of the light quantum number of 500-599nm to the total light quantum number of the light source is 12-18%, and the proportion of the light quantum number of 600-780nm to the total light quantum number of the light source is 65-77%.

Preferably, in the light source, the proportion of the number of 655 and 685nm light quanta to the total number of light quanta of the light source is 20-40%.

Preferably, the light period early stage of the full artificial light source is 12-14h/d, and the light intensity is 200-.

Further, the flowers include red, pink, blue and purple flowers.

The invention has the beneficial effects that: the invention is obtained by carrying out a large amount of creative tests on the basis that testers find that LED red lights with different wavelengths play a key role in regulating and controlling the flower color of herbaceous flowers during flower planting in plant factories, and the commercial value of the flowers can be realized by regulating and controlling the anthocyanin content to influence the flower color or extracting the anthocyanin for other industries such as food coloring, dyes, medicines, cosmetics and the like.

Detailed Description

The present invention will be described in further detail with reference to examples, but the embodiments of the present invention are not limited thereto, and various substitutions and alterations can be made without departing from the technical idea of the present invention as described above, according to the common technical knowledge and the conventional means in the field.

The embodiment of the invention provides a luminous environment regulation and control technology which is mainly used for regulating and controlling the anthocyanin content of red, pink, blue and purple flowers in plant factories. (1) Accelerating germination: the seeds are soaked in clear water for 3-6h, then sowed into sponge squares with 1 grain per hole, and placed into a germination accelerating box for accelerating germination, and after the seeds are exposed to white, the seeds are transferred to a full spectrum for culture. (2) Seedling culture: after 5-10cm of roots and 2 true leaves grow out, the seedlings are raised, the EC value of the nutrient solution is 0.8-1.2ms/cm during the treatment, and the pH value is 6.0-7.0. (3) Planting: when the seedlings grow to 4-5 leaves and 1 heart, carrying out cultivation and field planting, transferring the seedlings to a field planting plate, and putting the seedlings into a nutrient solution tank for cultivation, wherein the EC value of the nutrient solution is controlled to be 1.3-2.0 during field planting, and the pH value is 6.0-7.0. The temperature of the nutrient solution is 20-22 deg.C, dissolved oxygen amount is 5-6mg/L, the ambient temperature is 20-23 deg.C in daytime, 18-20 deg.C in night, air humidity is 60-70%, and CO is added₂The concentration is 800-1200 ppm.

The light source during the cultivation and planting period adopts the technical scheme that: the proportion of 400-499nm optical quantum number to the total optical quantum is controlled to be 7-12%, the proportion of 500-599nm optical quantum number to the total optical quantum is controlled to be 12-18%, the proportion of 600-780nm optical quantum number to the total optical quantum is controlled to be 65-80%, the proportion of 655-685nm optical quantum number to the total optical quantum is controlled to be 20-40%, the early stage of the optical cycle is 12-14h/d, and the intensity is adjusted to be 200-250 mu mol/square meter.

The technical solution of the present invention will be described in detail with reference to specific embodiments.

Example 1

Soaking seeds of the pelargonium sidoides in water, sowing the seeds into sponge blocks with 1 particle in each hole, then putting the sponge blocks into a germination accelerating box with the temperature of 23 ℃ for accelerating germination, after the seeds are exposed to white, transferring the seeds into a full spectrum for culture, and after 5-10cm roots and 2 true leaves grow out from the seedlings, carrying out seedling raising treatment, wherein the EC value of a nutrient solution during the treatment period is 0.8-1.2 ms/cm. Transferring into water culture nutrient solution for seedling culture management, wherein the EC value of the nutrient solution is 1.2ms/cm, the pH value is 6.0-7.0, and culturing till 5-6 true leaves are obtained. Selecting uniform seedlings, transplanting and field planting the seedlings on a field planting plate, culturing the seedlings in a nutrient solution tank, controlling the EC of the nutrient solution to be between 1.7 and 2.0ms/cm and the pH to be between 6.2 and 6.8, and simultaneously controlling the environmental temperature to be 21 ℃ in the daytime, 18 ℃ at night, 60 to 70 percent of air humidity and CO during the whole field planting period₂Concentration of 1000ppm and light intensity of 230. mu. mol. m^-2·s^-1The photoperiod is 12 h/d. The cultivation of geranium was carried out according to the above cultivation method, with the light source parameters as the respective examples. The test results are shown in table 1:

TABLE 1

The test results show that the pelargonium treated in examples 4-6 have a high anthocyanin content.

Example 2

Soaking seeds of the red corydalis cerana, sowing the seeds into sponge squares with 1 particle per hole, then putting the sponge squares into a germination accelerating box at 23 ℃ for accelerating germination, transferring the seeds into a full spectrum for culturing after the seeds are exposed to white, and performing seedling culture treatment after 5-10cm roots and 2 true leaves grow out from the seedlings, wherein the EC value of a nutrient solution during the treatment is 0.8-1.2 ms/cm. Transferring to water culture nutrient solution for seedling culture managementThe solution EC value is 1.2ms/cm, the pH value is 6.0-7.0, until 5-6 true leaves are cultivated. Selecting uniform seedlings, transplanting and field planting the seedlings on a field planting plate, culturing the seedlings in a nutrient solution tank, controlling the EC of the nutrient solution to be between 1.7 and 2.0ms/cm and the pH to be between 6.2 and 6.8, and simultaneously controlling the environmental temperature to be 21 ℃ in the daytime, 18 ℃ at night, 60 to 70 percent of air humidity and CO during the whole field planting period₂Concentration of 1000ppm and light intensity of 230. mu. mol. m^-2·s^-1The photoperiod is 12 h/d. The corydalis angulata was cultivated according to the above cultivation method, and the light source parameters were used as examples. The test results are shown in table 2:

TABLE 2

The test results show that the content of the goniochronocyanin treated in examples 6 to 7 is higher.

Embodiment 3

Soaking bramble color Shiraia bambusicola seeds, sowing the seeds into sponge blocks with 1 particle per hole, putting the sponge blocks into a germination accelerating box with the temperature of 23 ℃ for accelerating germination, transferring the seeds into a full spectrum for culturing after the seeds are exposed to the white, and performing seedling culture treatment after 5-10cm roots and 3-4 true leaves grow out from the seedlings, wherein the EC value of a nutrient solution is 1.0ms/cm during the treatment period, and the pH value is 6.5. Transferring into water culture nutrient solution for seedling culture management, wherein the EC value of the nutrient solution is 1.2ms/cm, the pH value is 6.0-6.5, and culturing till 5-6 true leaves are obtained. Selecting uniform seedlings, transplanting and field planting the seedlings on a field planting plate, culturing the seedlings in a nutrient solution tank, controlling the EC of the nutrient solution to be between 1.5 and 1.8ms/cm and the pH to be between 6.5 and 7.0, and simultaneously controlling the environmental temperature to be 21 ℃ in the daytime, 18 ℃ at night, 60 to 70 percent of air humidity and CO during the whole field planting period₂The concentration is 1000ppm, the light intensity is 210-^-2·s^-1The photoperiod is 12 h/d. The carnation was cultivated according to the above cultivation method with the light source parameters as each example. The results of the experiment are shown in table 3:

TABLE 3

The test results show that the content of the anthocyanin in the pink treated in the examples 7 to 9 is higher.

Example 4

Soaking seeds of the blue five-star platycodon grandiflorum, sowing the seeds into sponge squares with 1 particle in each hole, then putting the sponge squares into a germination accelerating box at 23 ℃ for accelerating germination, transferring the seeds into a full spectrum for culturing after the seeds are exposed to the white, and performing seedling culture treatment after 5-10cm roots and 3-4 true leaves grow out of seedlings, wherein the EC value of a nutrient solution is 1.0ms/cm during the treatment period, and the pH value is 6.5. Transferring into water culture nutrient solution for seedling culture management, wherein the EC value of the nutrient solution is 1.2ms/cm, the pH value is 6.0-6.5, and culturing till 5-6 true leaves are obtained. Selecting uniform seedlings, transplanting and field planting the seedlings on a field planting plate, culturing the seedlings in a nutrient solution tank, controlling the EC of the nutrient solution to be between 1.5 and 1.8ms/cm and the pH to be between 6.5 and 7.0, and simultaneously controlling the environmental temperature to be 21 ℃ in the daytime, 18 ℃ at night, 60 to 70 percent of air humidity and CO during the whole field planting period₂The concentration is 1000ppm, the light intensity is 210-^-2·s^-1The photoperiod is 12 h/d. The carnation was cultivated according to the above cultivation method with the light source parameters as each example. The results of the experiment are shown in table 4:

TABLE 4

The test results show that the platycodon grandiflorum processed in examples 5-6 has high anthocyanin content.

Although the embodiments have been described, once the basic inventive concept is known, other variations and modifications can be made to the embodiments by those skilled in the art, so that the above embodiments are only examples of the present invention, and not intended to limit the scope of the present invention, and all equivalent structures or equivalent processes that can be used in the present specification or directly or indirectly applied to other related fields are encompassed by the present invention.

Claims

1. A method for promoting the anthocyanin content accumulation of plant plants is characterized in that the wavelength range of a full artificial light source is 380-780nm, wherein the proportion of light quanta at 380-499nm to the total light quantum number of the light source is 7-12%, the proportion of light quanta at 500-599nm to the total light quantum number of the light source is 12-18%, and the proportion of light quanta at 600-780nm to the total light quantum number of the light source is 65-80%.

2. The method as claimed in claim 1, wherein the ratio of the number of light quanta with 655-685nm to the total number of light quanta of the light source is 20-40%.

3. The method for promoting the accumulation of the anthocyanin content in the plant plants as claimed in claim 1 or 2, wherein the previous period of the light cycle of the total artificial light source is 12-14h/d, and the light intensity is 200-.

4. The method for promoting the accumulation of anthocyanin content in plant factory flowers as claimed in claim 1 or 2, wherein the flowers comprise red, pink, blue and purple flowers.