CN102939841A - Method for improving anthocyanidin contents in purple white birch leaves - Google Patents
Method for improving anthocyanidin contents in purple white birch leaves Download PDFInfo
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- CN102939841A CN102939841A CN2012104787303A CN201210478730A CN102939841A CN 102939841 A CN102939841 A CN 102939841A CN 2012104787303 A CN2012104787303 A CN 2012104787303A CN 201210478730 A CN201210478730 A CN 201210478730A CN 102939841 A CN102939841 A CN 102939841A
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Abstract
The invention discloses a method for improving anthocyanidin contents in purple white birch leaves, relates to a seedling cultivation technology and mainly solves problems that the purple white birch leaves are poor in color stability and the leaf color is easily influenced by environmental conditions to change. The method includes cultivating a seedling of a purple white birch potted plant under conditions of appropriate illumination intensity, humidity and temperatures, and irrigating the seedling and applying fertilizers to the seedlings in appropriate amount during the cultivation period. The method for improving the anthocyanidin contents in the purple white birch leaves is simple in cultivation method, high in operability and obvious in effects; and by means of the method, purple characteristics of the purple white birch leaves are all kept, the anthocyanidin contents are improved by 2 times of those of the original, the purple white birch leaves are high in color stability, the color of the purple white birch leaves is not easily influenced by the environmental conditions to change, seedlings can grow in a healthy and strong mode, and the target of effectively keeping purple characteristics of the purple white birch leaves can be achieved.
Description
Technical field
The present invention relates to a kind of nursery technology.
Background technology
White birch with purple leaf (Betula populifolia x Whitespire ' Royal Frost ') is drooping birch and Asia white birch through hybridization repeatedly and according to leaf color characteristic long-term breeding new varieties out, English " Betula Royal Frost Penci-2 " by name.White birch with purple leaf is the suitable fine tree species of viewing and admiring throughout the year, its blade remains the wine redness in whole spring and summer, then become yellow, orange-yellow or orange red autumn, behind the winter leaf abscission, its cinnamon to the trunk of the shape that scales off of white has very high ornamental value equally.It is strong that white birch with purple leaf also has resistance to insects, happiness illumination, humidity, wide adaptability, the easily characteristics of plantation.
External white birch with purple leaf nursery stock public offering is existing historical for many years, and for example just there was the public offering of white birch with purple leaf nursery stock in U.S. Evergreen nursery from 2002; U.S. Mountain Shadow nursery began to introduce and the public offering white birch with purple leaf in 2004.At present, Australia has also successfully grasped cultivation and the planting technology of these seeds.The cultivation of the white birch with purple leaf of China starts from 2007, by organizing the cultivation means to set up the little propagating technology method of white birch with purple leaf, can realize white birch with purple leaf fast, the efficient target of breeding and large-scale promotion plantation.At present, research and production through the white birch with purple leaf micro-propagation method, existing white birch with purple leaf in vitro cuttings more than ten thousand strains, living nursery stock strain more than 2000 in potted plant 3 years after the domestication, Wild Ornamental trees domestication Breeding bases (in the Shangzhi City, Heilongjiang Province) carry out the Extend culture of white birch with purple leaf nursery stock northeastward; And because the leaf look characteristics of white birch with purple leaf make it have very high ornamental value, on the domestic market white birch with purple leaf nursery stock supply falls short of demand.But in the stock growing process after rooting of vitro seedling survives, find the leaf color poor stability of white birch with purple leaf, the impact that the leaf look is subject to environmental condition occurs significantly to change, and blade loses the purple feature, becomes green or bottle green.This has limited in the Extend culture of white birch with purple leaf and the afforestation and has used.
Summary of the invention
The objective of the invention is in order to solve white birch with purple leaf leaf color poor stability, the leaf look is subject to the problem that environmental influence changes, and a kind of method that improves anthocyanidin content in the white birch with purple leaf blade that provides.
Improve the method for anthocyanidin content in the white birch with purple leaf blade and press following process implementation:
The nursery stock that white birch with purple leaf is potted plant is in intensity of illumination that 3700～20000Lx, humidity are 60%～80%, temperature is to continue to cultivate 20～30 days under 20～25 ℃ the condition, be that 6.5～7.5 water waters potted plant nursery stock with the pH value between culture period, the method by foliage-spray between culture period is 0.5%～1.0% K with mass concentration
2SO
4Solution sprays application on the white birch with purple leaf blade surface, sprays once every 3 days, sprays continuously 3～5 times, and each wetting blade face gets final product; Namely finish the method that improves anthocyanidin content in the white birch with purple leaf blade.
Improve the method for anthocyanidin content in the white birch with purple leaf blade among the present invention, cultural method is simple, workable, successful, the blade of white birch with purple leaf all keeps the purple feature, anthocyanidin content rises to original 2 times, white birch with purple leaf leaf color stability is strong, the leaf look is not subject to environmental influence and changes, and seedling growth is healthy and strong, can realize effectively keeping the target of white birch with purple leaf blade purple feature.
Embodiment
Embodiment one: present embodiment improves the method for anthocyanidin content in the white birch with purple leaf blade and presses following process implementation:
The nursery stock that white birch with purple leaf is potted plant is in intensity of illumination that 3700～20000Lx, humidity are 60%～80%, temperature is to continue to cultivate 20～30 days under 20～25 ℃ the condition, be that 6.5～7.5 water waters potted plant nursery stock with the pH value between culture period, the method by foliage-spray between culture period is 0.5%～1.0% K with mass concentration
2SO
4Solution sprays application on the white birch with purple leaf blade surface, sprays once every 3 days, sprays continuously 3～5 times, and each wetting blade face gets final product; Namely finish the method that improves anthocyanidin content in the white birch with purple leaf blade.
The potted plant nursery stock of white birch with purple leaf in the present embodiment, its height are more than or equal to 5 centimetres, and leading thread has the blade that launches fully more than 4 more than or equal to 0.15 centimetre, and leaf color is purple or bottle green.
White birch with purple leaf in the present embodiment is that the public can be by the biomaterial of commercial channel from buying both at home and abroad, and is just to sell to the public in 2002; Purchase information is as follows: the Evergreen nursery of Wisconsin, USA (http://www.evergreennursery.com/).
Illumination condition in the present embodiment between culture period is natural daylight or artificial lighting.
Embodiment two: present embodiment and embodiment one be not both that the nursery stock that white birch with purple leaf is potted plant is in intensity of illumination that 3700Lx, humidity are 60%, temperature is to continue to cultivate 30 days under 25 ℃ the condition.Other step and parameter are identical with embodiment one.
Embodiment three: present embodiment and embodiment one be not both that the nursery stock that white birch with purple leaf is potted plant is in intensity of illumination that 20000Lx, humidity are 80%, temperature is to continue to cultivate 20 days under 20 ℃ the condition.Other step and parameter are identical with embodiment one.
Embodiment four: present embodiment and embodiment one be not both that the nursery stock that white birch with purple leaf is potted plant is in intensity of illumination that 10000Lx, humidity are 70%, temperature is to continue to cultivate 25 days under 24 ℃ the condition.Other step and parameter are identical with embodiment one.
Embodiment five: present embodiment and embodiment one not to be both between culture period with the pH value be that 7.0 water waters potted plant nursery stock.Other step and parameter are identical with embodiment one.
Embodiment six: the method by foliage-spray of not being both between culture period of present embodiment and embodiment one is 0.8% K with mass concentration
2SO
4Solution sprays application on the white birch with purple leaf blade surface, sprays once every 3 days, sprays continuously 4 times, and each wetting blade face gets final product.Other step and parameter are identical with embodiment one.
Embodiment 1:
Improve the method for anthocyanidin content in the white birch with purple leaf blade and press following process implementation:
The nursery stock that white birch with purple leaf is potted plant is in intensity of illumination that 10000Lx, humidity are 80%, temperature is to continue to cultivate 30 days under 20 ℃ the condition, be that 7.0 water waters potted plant nursery stock with the pH value between culture period, the method by foliage-spray between culture period is 0.8% K with mass concentration
2SO
4Solution sprays application on the white birch with purple leaf blade surface, sprays once every 3 days, sprays continuously 5 times, and each wetting blade face gets final product; Namely finish the method that improves anthocyanidin content in the white birch with purple leaf blade.
The blade of the potted plant nursery stock of gained white birch with purple leaf all keeps the purple feature in the present embodiment, and the anthocyanidin content in its top vane is brought up to 1.45U.g
-1.mL
-1, be more than 2 times of top vane anthocyanidin content that existing conventional rooting of vitro seedling survives the potted plant white birch with purple leaf nursery stock of rear identical seedling age.
Embodiment 2:
Improve the method for anthocyanidin content in the white birch with purple leaf blade and press following process implementation:
The nursery stock that white birch with purple leaf is potted plant is in intensity of illumination that 10000Lx, humidity are 70%, temperature is to continue to cultivate 25 days under 22 ℃ the condition, be that 7.0 water waters potted plant nursery stock with the pH value between culture period, the method by foliage-spray between culture period is 0.7% K with mass concentration
2SO
4Solution sprays application on the white birch with purple leaf blade surface, sprays once every 3 days, sprays continuously 4 times, and each wetting blade face gets final product; Namely finish the method that improves anthocyanidin content in the white birch with purple leaf blade.The blade of the potted plant nursery stock of gained white birch with purple leaf all keeps the purple feature in the present embodiment, and the anthocyanidin content in its top vane is brought up to 1.4U.g
-1.mL
-1, be more than 2 times of top vane anthocyanidin content that existing conventional rooting of vitro seedling survives the potted plant white birch with purple leaf nursery stock of rear identical seedling age.
Claims (6)
1. method that improves anthocyanidin content in the white birch with purple leaf blade is characterized in that it is by following process implementation:
The nursery stock that white birch with purple leaf is potted plant is in intensity of illumination that 3700～20000Lx, humidity are 60%～80%, temperature is to continue to cultivate 20～30 days under 20～25 ℃ the condition, be that 6.5～7.5 water waters potted plant nursery stock with the pH value between culture period, the method by foliage-spray between culture period is 0.5%～1.0% K with mass concentration
2SO
4Solution sprays application on the white birch with purple leaf blade surface, sprays once every 3 days, sprays continuously 3～5 times, and each wetting blade face gets final product; Namely finish the method that improves anthocyanidin content in the white birch with purple leaf blade.
2. a kind of method that improves anthocyanidin content in the white birch with purple leaf blade according to claim 1 is characterized in that the nursery stock that white birch with purple leaf is potted plant is in intensity of illumination that 3700Lx, humidity are 60%, temperature is to continue to cultivate 30 days under 25 ℃ the condition.
3. a kind of method that improves anthocyanidin content in the white birch with purple leaf blade according to claim 1 is characterized in that the nursery stock that white birch with purple leaf is potted plant is in intensity of illumination that 20000Lx, humidity are 80%, temperature is to continue to cultivate 20 days under 20 ℃ the condition.
4. a kind of method that improves anthocyanidin content in the white birch with purple leaf blade according to claim 1 is characterized in that the nursery stock that white birch with purple leaf is potted plant is in intensity of illumination that 10000Lx, humidity are 70%, temperature is to continue to cultivate 25 days under 24 ℃ the condition.
5. a kind of method that improves anthocyanidin content in the white birch with purple leaf blade according to claim 1 is characterized in that between culture period with the pH value being that 7.0 water waters potted plant nursery stock.
6. a kind of method that improves anthocyanidin content in the white birch with purple leaf blade according to claim 1 is characterized in that between culture period that method by foliage-spray is 0.8% K with mass concentration
2SO
4Solution sprays application on the white birch with purple leaf blade surface, sprays once every 3 days, sprays continuously 4 times, and each wetting blade face gets final product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210478730 CN102939841B (en) | 2012-11-22 | 2012-11-22 | Method for improving anthocyanidin contents in purple white birch leaves |
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| CN 201210478730 CN102939841B (en) | 2012-11-22 | 2012-11-22 | Method for improving anthocyanidin contents in purple white birch leaves |
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| CN102939841A true CN102939841A (en) | 2013-02-27 |
| CN102939841B CN102939841B (en) | 2013-11-06 |
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| CN 201210478730 Expired - Fee Related CN102939841B (en) | 2012-11-22 | 2012-11-22 | Method for improving anthocyanidin contents in purple white birch leaves |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105325168A (en) * | 2015-11-17 | 2016-02-17 | 山东省潍坊市农业科学院 | Cultivation method of asparagus rich in anthocyanidin and light-diffusing, heat-insulation and heat-radiation regulation and control film specially used for cultivation method |
| CN106962117A (en) * | 2017-04-14 | 2017-07-21 | 贵州省黄平县吉兴生态科技有限公司 | The implantation methods of blueberry |
| CN111642310A (en) * | 2020-05-25 | 2020-09-11 | 天津泰达盐碱地绿化研究中心有限公司 | Cultivation method for improving red leaf character of Euonymus alatus and prolonging ornamental period |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1084151A (en) * | 1993-10-11 | 1994-03-23 | 戴希泽 | A kind of fruit tree leaves leaven |
| CN1123264A (en) * | 1994-11-18 | 1996-05-29 | 陕西省土壤肥料研究所 | Fertilizer for making fruit fleshy and coloured and producing method thereof |
| CN1258329A (en) * | 1997-05-28 | 2000-06-28 | 斯蒂芬·B·奥格 | Mineral stains for wood and other substrates |
| CN101283685A (en) * | 2008-06-10 | 2008-10-15 | 辽宁石油化工大学 | Preparation and producing method for promoting the Nanguo pear coloring |
-
2012
- 2012-11-22 CN CN 201210478730 patent/CN102939841B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1084151A (en) * | 1993-10-11 | 1994-03-23 | 戴希泽 | A kind of fruit tree leaves leaven |
| CN1123264A (en) * | 1994-11-18 | 1996-05-29 | 陕西省土壤肥料研究所 | Fertilizer for making fruit fleshy and coloured and producing method thereof |
| CN1258329A (en) * | 1997-05-28 | 2000-06-28 | 斯蒂芬·B·奥格 | Mineral stains for wood and other substrates |
| CN101283685A (en) * | 2008-06-10 | 2008-10-15 | 辽宁石油化工大学 | Preparation and producing method for promoting the Nanguo pear coloring |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105325168A (en) * | 2015-11-17 | 2016-02-17 | 山东省潍坊市农业科学院 | Cultivation method of asparagus rich in anthocyanidin and light-diffusing, heat-insulation and heat-radiation regulation and control film specially used for cultivation method |
| CN105325168B (en) * | 2015-11-17 | 2018-05-22 | 山东省潍坊市农业科学院 | A kind of asparagus cultivation method rich in anthocyanidin |
| CN106962117A (en) * | 2017-04-14 | 2017-07-21 | 贵州省黄平县吉兴生态科技有限公司 | The implantation methods of blueberry |
| CN111642310A (en) * | 2020-05-25 | 2020-09-11 | 天津泰达盐碱地绿化研究中心有限公司 | Cultivation method for improving red leaf character of Euonymus alatus and prolonging ornamental period |
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| CN102939841B (en) | 2013-11-06 |
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