CN106718182A - A kind of method coloured to strawberry fruit - Google Patents
A kind of method coloured to strawberry fruit Download PDFInfo
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- CN106718182A CN106718182A CN201611087351.6A CN201611087351A CN106718182A CN 106718182 A CN106718182 A CN 106718182A CN 201611087351 A CN201611087351 A CN 201611087351A CN 106718182 A CN106718182 A CN 106718182A
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- light
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/04—Electric or magnetic or acoustic treatment of plants for promoting growth
- A01G7/045—Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/14—Measures for saving energy, e.g. in green houses
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- Life Sciences & Earth Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a kind of method coloured to strawberry fruit, the method includes：Strawberry fruit is coloured using red and blue LED light；Wherein, the ratio of feux rouges and blue light is 1~3:1~3, red light wavelength is 700~750nm, and blue light wavelength is 420~480nm；Use the detailed process that red and blue LED light is coloured to strawberry for：Strawberry after blooming 6~8 days is placed under conditions of relative humidity 72%~78%, 20~25 DEG C of day temperature, 14~17 DEG C of evening temperature, is processed 18~28 days using red and blue LED light, 15~17h is processed daily；Wherein, the luminous intensity of red and blue LED light is 5400~5600lx.Under the conditions of specific light quality, light intensity, light application time and direction of illumination, strawberry fruit can be promoted to colour in advance, improve its anthocyanidin content.
Description
Technical field
Field is cultivated the present invention relates to fruit, and in particular to a kind of method coloured to strawberry fruit.
Background technology
Strawberry (Fragaria × ananassa, Duch.) is rose family Fragaria herbaceos perennial, its cultivated area
Grape is only second to yield, world's berry area and yield second is occupied, because its fruit is fresh and tender, bright color, soft and succulency, battalion
Support abundant, be referred to as " fruit queen ".Strawberry winter-spring season starts to bear fruit, and southern area is often in the weather bar of low temperature and poor light
Part, while facility interior lights are particularly acute according to not enough and light quality composition energy imbalance, does not fill so as to cause strawberry fruit to colour
Point, it is uneven.
The color and luster of fruit directly affects its apparent quality and commodity, and strawberry fruit coloring mainly contains with anthocyanin in fruit
Amount is relevant with species.The synthesis of anthocyanidin is regulated and controled by various environmental factors and development signal, just topmost outside regulation
The factor, light is different to, influence to it of light intensity, light quality and photoperiod, and wherein light quality plays even more important effect.
It is the conventional method for carrying out light quality regulating fruit coloring using color plastic film for agricultural use, fruit bag.Meanwhile, plant conventional facilities are planted
It is also adopted by different light sources such as incandescent lamp, fluorescent lamp, high-pressure mercury lamp and metal halide lamp in training, but these light source lifes are short, hair
Heat is big, there is many unnecessary wavelength in spectrum, and inefficiency causes strawberry fruit to colour insufficient, uneven.As
Four generation novel illumination light sources, light emitting diode has small volume, and long lifespan, the low, spectrum property that consumes energy are good, can combine as required
Obtain pure monochromatic light and complex spectrum, as cold light source can with irradiation at short distance plant the advantages of, therefore be very suitable for application
Plant culture in controllable facilities environment.
Major part scholar studies carefully and thinks both at home and abroad, and red blue light is conducive to growing for plant, and the photosynthetic efficiency of plant growth is most
Radiation scope high concentrates on red light district and blue light region, and especially blue light is the essential light source of growth and development of plants, therefore feux rouges,
Blue light and red blue mixed light are the study hotspot in facility cultivation, such as red blue-light source answering in terms of paddy rice, cherry, tomato
With.
At present, during red blue light is mainly used in plant seedling growth, and application in fruit development and its coloring is not
Extensively.
The content of the invention
For above-mentioned deficiency of the prior art, the present invention provides a kind of method coloured to strawberry fruit, and it has
It is relatively low that effect solves the problems, such as that the upper strawberry fruit of production colours anthocyanidin content in uneven and strawberry.
To solve above-mentioned technical problem, the technical solution adopted by the present invention is：
A kind of method coloured to strawberry fruit, the method includes：
Strawberry fruit is coloured using red and blue LED light；Wherein, the ratio of feux rouges and blue light is 1~3:1~3, feux rouges
Wavelength is 700~750nm, and blue light wavelength is 420~480nm.
Further, by the strawberry after blooming 6~8 days be placed in relative humidity 72%~78%, 20~25 DEG C of day temperature,
Under conditions of 14~17 DEG C of evening temperature, processed 18~28 days using red and blue LED light, 15~17h is processed daily；Wherein, red indigo plant
The luminous intensity of LED light is 5400~5600lx.
Further, feux rouges and blue light ratio are 1 in red and blue LED light:1.
Further, red light wavelength is 730nm, and blue light wavelength is 450nm.
Further, red and blue LED light is placed in strawberry upper end, makes its direction of illumination vertical with strawberry.
Beneficial effects of the present invention are：
Using red and blue LED light to spending rear strawberry to be irradiated, in specific light quality, light intensity, light application time and illumination side
Under the conditions of, strawberry fruit can be promoted to colour in advance, lift the uniformity of fruit color, improve the outward appearance of fruit, and can also
Anthocyanidin content in fruit is improved, the coloring effect of fruit is lifted, and then lifts its quality.
Brief description of the drawings
Fig. 1 is ' Snow White ' strawberry fruit brightness contrast figure under different light medium treatment；
Fig. 2 is ' Snow White ' strawberry fruit color saturation comparison diagram under different light medium treatment；
Fig. 3 is ' Snow White ' strawberry fruit colourity angle comparison diagram under different light medium treatment；
Fig. 4 is ' Snow White ' strawberry fruit champac value comparison diagram under different light medium treatment；
Fig. 5 is the red value of green comparison diagram of ' Snow White ' strawberry fruit under different light medium treatment；
Fig. 6 is anthocyanidin content in " Snow White " strawberry fruit after pH shows poor method determines different light medium treatment；
Fig. 7 is pelargonin -3- glucoside standard curves；
Fig. 8 is sample chromatogram figure；
Fig. 9 is anthocyanidin content in ' Feng Xiang ' strawberry fruit under different light medium treatment.
Specific embodiment
Specific embodiment of the invention is described below, this hair is understood in order to those skilled in the art
It is bright, it should be apparent that the invention is not restricted to the scope of specific embodiment, for those skilled in the art,
As long as in appended claim restriction and the spirit and scope of the present invention for determining, these changes are aobvious and easy to various change
See, all are using the innovation and creation of present inventive concept in the row of protection.
Embodiment 1
A kind of method coloured to strawberry fruit, comprises the following steps：
(1) from cultivated strawberry kind ' Snow White ', by its seedling cultivation in a diameter of 15cm, the flowerpot of 13cm high
In, its cultivation matrix is by volume 2 by Nutrition Soil, rural area soil, perlite:2:1 mixes, and per potted plant 2 plants of training, then puts
In being cultivated in greenhouse, growth period ensures that its moisture supply is sufficient, and routinely way to manage is carried out for other operations；
(2) after strawberry blooms 7 days, relative humidity 75%, (morning 5 on daytime are placed on:00 to evening 21:00) temperature
25 DEG C, in (evening 21 in the evening:00 to the next morning 5:00) in the growth cabinet of 16 DEG C of temperature, be respectively adopted white light, feux rouges,
Blue light and red blue light illumination strawberry 28 days, daily light application time section is morning 5:00 to evening 21:00, common 16h, every kind of light quality
Process 20 basins；Wherein, white light, feux rouges, blue light and red blue light light intensity are 5500lx；Every kind of light quality is disposed as LED illumination
Plate, is placed in artificial climate box top, and vertical illumination is carried out to strawberry, so as to improve the utilization rate of light, is conducive to strawberry to grow；It is red
Feux rouges and blue light ratio are 1 in blue light:1, red light wavelength is 730nm, and blue light wavelength is 450nm；
(3) respectively in Post flowering 14d (14DAF), 21d (21DAF), 28d (28DAF), 31d (31DAF) and 35d
(35DAF) starts strawberrying fruit.
After ' Snow White ' maturation, its appearance and pulp are in white powder, there is red seed.
Sample has been adopted every time, the colourity of fruit has been determined with color difference meter immediately, and assay method has been choose fruit equator relative four
Point, takes its average value as fruit colourity, each treatment 5 strawberry fruit (randomly selecting) of measure, and its measurement result such as Fig. 1~
Shown in 5.
Its anthocyanidin content is determined after having determined immediately or liquid nitrogen flash freezer is used, in being preserved at -80 DEG C, then surveyed again
Its anthocyanidin content.
The assay method of anthocyanidin content：Show that poor method is determined using pH.Anthocyanidin content is with anthocyanidin day in strawberry fruit
The molar absorption coefficient (15600) of Zhu Kui -3- glucosides is calculated, and the amount (mg) for finally containing anthocyanidin with 1g samples carrys out table
Show, when pH is 1.0, respectively at the absorbance value that anthocyanidin fish pelargonium -3- glucosides are determined under 496nm and 700nm；Work as pH
For 4.5 when, respectively under 496nm and 700nm determine anthocyanidin fish pelargonium -3- glucosides absorbance value, measurement result is such as
Shown in Fig. 6.
Computing formula is：
Wherein A=(A496nm-A700nm)PH=1.0-(A496nm-A700nm)PH=4.5；
MW is molal weight (433.2g/mol)；
DF is extension rate；
∑ is absorptivity (15600)；
L is optical path 1cm.
Embodiment 2
A kind of method coloured to strawberry fruit, comprises the following steps：
(1) from cultivated strawberry kind ' Feng Xiang ', by its seedling cultivation in a diameter of 15cm, the flowerpot of 13cm high, its
Cultivation matrix is by volume 2 by Nutrition Soil, rural area soil, perlite:2:1 mixes, and per potted plant 2 plants of training, is subsequently placed in big
Culture in canopy, growth period ensures that its moisture supply is sufficient, and routinely way to manage is carried out for other operations；
(2) after strawberry blooms 7 days, relative humidity 75%, (morning 5 on daytime are placed on:00 to evening 21:00) temperature
25 DEG C, in (evening 21 in the evening:00 to the next morning 5:00) in the growth cabinet of 16 DEG C of temperature, be respectively adopted white light, feux rouges,
Blue light and red blue light illumination strawberry 21 days, daily light application time section is morning 5:00 to evening 21:00, common 16h, each treatment
20 basins are set；Wherein, white light, feux rouges, blue light and red blue light light intensity are 5500lx；Every kind of light quality is disposed as LED illumination
Plate, is placed in artificial climate box top, and vertical illumination is carried out to strawberry, so as to improve the utilization rate of light, is conducive to strawberry to grow；It is red
Feux rouges and blue light ratio are 1 in blue light:1, red light wavelength is 730nm, and blue light wavelength is 450nm；
(3) collection grass is started in Post flowering 14d (14DAF), 21d (21DAF), 25d (25DAF) and 28d (28DAF) respectively
Certain kind of berries fruit.
After ' Feng Xiang ' maturation, its outward appearance is gorgeous in cerise, and pulp is in light red, and seed is small, is yellow green or red.
Sample has been adopted every time, its anthocyanidin content has been determined immediately, or used liquid nitrogen flash freezer, in being preserved at -80 DEG C, then
Its anthocyanidin content is surveyed again.
The assay method of anthocyanidin content：Determined using high performance liquid chromatography (HPLC), that is, weigh 1.0g strawberry fruits,
Fine powder is ground to liquid nitrogen, the HCL- methyl alcohol of 5mL1% is subsequently adding, in 4 DEG C of extractions overnight, centrifugation, filtering, residue is again
Extracted 2 times with 1% HCL- methyl alcohol, merge supernatant, be loaded into 10mL brown volumetric flasks and constant volume.Therefrom draw appropriate
Extract solution, crosses 0.45 μm of organic filtering with microporous membrane, for liquid chromatographic detection.
Using Agilent1260 high performance liquid chromatography, VWD detectors, Detection wavelength is 520nm, and column temperature is 30 DEG C.
Chromatographic column：ZORBAX SB-C18 (150 × 4.6mm, 5 μm).
3 pure mobile phases：A：Water, B：Acetonitrile, C：Formic acid；
Elution requirement：Carry out gradient elution, 0min, 100%A；13min, 78%A+20%B+2%C；20min, 58%A+
40%B+2%C；25min, 100%A, flow velocity 1mL/min.
Sampling volume：10μL.
To be 0 purchased from the pelargonin -3- glucosides HCL- methanol dilutions that add 1% of sigma companies, 2.5,5,10,
20th, the gradient solution of 50,100 μ g/mL, with its concentration as abscissa, peak area is ordinate, draws standard curve, is marked
Directrix curve equation：Y=27.08X+3.3157, its coefficient R2=0.9998, then bring the peak area of sample into standard curve
In, calculate anthocyanin concentrations in sample；The standard curve of pelargonin -3- glucosides is as shown in Figure 7.
Interpretation of result
As shown in Fig. 1~5, L represents brightness, and a represents red value of green, and b represents champac value, and C represents color saturation, and h is represented
Colourity angle, data are expressed as average value ± standard error (n=3), and the different letters of contemporaneity represent significant difference (P in figure<
0.05)。
With the development of strawberry fruit, after different light medium treatment, its surface color is changed significantly.Early stage different disposal
Under the red value of green a of fruit be negative, illustrate fruit also in green fruiting period.The brightness L of rear 21d, each lower fruit of light quality treatment is spent to raise
And feux rouges, blue light and red blue light are significantly higher than control white light, show that fruit starts chlorisis, and feux rouges, blue light and red blue light are brighter
Aobvious, this is consistent with macroscopic phenomenon, has had a different degrees of coloring, wherein feux rouges to 28d strawberry fruits, blue light and red
The brightness L of blue light, colourity angle h is substantially reduced, while red value of green a, color saturation C are significantly higher than control white light.From Fig. 1
It is also seen that after fruit colour generation, a values have a process for decline, fruit color shoals, but fruit under feux rouges, blue light and red blue light
Real L, C are less than white light, illustrate its fruit color heterozygosis, and species may be more.
As shown in fig. 6, wherein, WL：White light, RL：Feux rouges, BL：Blue light, RBL：Red blue light, for pH shows, poor method determines grass to Fig. 2
The result of anthocyanidin content in certain kind of berries fruit, pH shows that poor method shows, does not have anthocyanidin to accumulate in 14d and 21d after spending, in strawberry fruit
Tired, since strawberry accumulate anthocyanidin spending rear 28d, and through the strawberry fruit after the treatment of feux rouges, blue light and red blue light, in it
Anthocyanidin content is significantly higher than control white light, but anthocyanidin content in strawberry fruit under later stage feux rouges is less than control white light,
Blue light is higher than white light, but not significantly, and maintain content higher under red blue light always and be significantly higher than other three kinds of light qualities.With
On show, blue light and red blue light can improve the content of strawberry anthocyanidin, wherein the most notable with the effect of red blue light.
From Fig. 1~6, feux rouges, blue light and red blue light treatment each contribute to strawberry fruit do sth. in advance coloring, and blue light and
Red blue light can improve the content of strawberry anthocyanidin, especially red blue light best results.
Fig. 7 is the standard curve of pelargonin -3- glucosides (Pg3G), and Fig. 8 is sample chromatogram figure, wherein, standard is bent
The coefficient R of line2=0.9998, show that it has good linear relationship, from the chromatogram of sample it can also be seen that the survey
Determine that method baseline is steady, appearance stabilization, the separating degree of target component is good, is a kind of reliable analysis method, be suitable for strawberry and
The measure of other plant Pg3G.
Fig. 9 is the anthocyanidin content variation diagram in " Feng Xiang " strawberry fruit under different light medium is processed, wherein, WL：White light,
RL：Feux rouges, BL：Blue light, RBL：Red blue light, it can be seen that " Feng Xiang " strawberry is just gradually developed the color after 21d of blooming,
And the lower anthocyanidin content of different light medium treatment has difference, in 25d after spending, anthocyanidin content relation is in strawberry fruit：It is blue
Light>Feux rouges>Red blue light>White light, and the later stage red blue light treatment under, the anthocyanidin content in strawberry fruit is significantly higher than other light
Matter, content is minimum under feux rouges.More than explainable feux rouges, blue light and red blue light strawberry can be allowed to do sth. in advance coloring, but later stage blue light and
Red blue light is more conducive to anthocyanin accumulation than control white light, wherein red blue light best results.
Claims (5)
1. a kind of method coloured to strawberry fruit, it is characterised in that including：
Strawberry fruit is coloured using red and blue LED light；Wherein, the ratio of feux rouges and blue light is 1~3:1~3, red light wavelength
It is 700~750nm, blue light wavelength is 420~480nm.
2. the method coloured to strawberry according to claim 1, it is characterised in that using red and blue LED light to strawberry
The detailed process for being coloured is：
By the strawberry after blooming 6~8 days be placed in relative humidity 72%~78%, 20~25 DEG C of day temperature, evening temperature 14~
Under conditions of 17 DEG C, processed 18~28 days using red and blue LED light, 15~17h is processed daily；Wherein, the luminous intensity of red and blue LED light
It is 5400~5600lx.
3. the method coloured to strawberry fruit according to claim 1 and 2, it is characterised in that the red and blue LED light
Middle feux rouges and blue light ratio are 1:1.
4. the method coloured to strawberry fruit according to claim 3, it is characterised in that the red light wavelength is
730nm, blue light wavelength is 450nm.
5. the method coloured to strawberry fruit according to any one of Claims 1 to 4, it is characterised in that：Will be described
Red and blue LED light is placed in strawberry upper end, makes its direction of illumination vertical with strawberry.
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Cited By (5)
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| CN107736438A (en) * | 2017-10-17 | 2018-02-27 | 成都新柯力化工科技有限公司 | A kind of method for promoting Apple fragrance and coloring using LED |
| CN113099878A (en) * | 2021-03-24 | 2021-07-13 | 福建省中科生物股份有限公司 | Method for promoting anthocyanin content accumulation of plant factory flowers |
| CN113498689A (en) * | 2021-07-09 | 2021-10-15 | 福建省中科生物股份有限公司 | Luminous environment for indoor purple and red lettuce production |
| CN113597914A (en) * | 2021-08-13 | 2021-11-05 | 福建省中科生物股份有限公司 | Luminous environment for promoting succulent plant coloring |
| CN114847099A (en) * | 2022-06-02 | 2022-08-05 | 中国科学院长春应用化学研究所 | Light control method for strawberry growth |
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| CN113498689B (en) * | 2021-07-09 | 2023-08-11 | 福建省中科生物股份有限公司 | Light environment for producing indoor purple and red lettuce |
| CN113597914A (en) * | 2021-08-13 | 2021-11-05 | 福建省中科生物股份有限公司 | Luminous environment for promoting succulent plant coloring |
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Application publication date: 20170531 |