CN107410225A - A kind of rearing method of scale benefit stinkbug - Google Patents
A kind of rearing method of scale benefit stinkbug Download PDFInfo
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- CN107410225A CN107410225A CN201710937580.0A CN201710937580A CN107410225A CN 107410225 A CN107410225 A CN 107410225A CN 201710937580 A CN201710937580 A CN 201710937580A CN 107410225 A CN107410225 A CN 107410225A
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- 241000578422 Graphosoma lineatum Species 0.000 title claims abstract description 103
- 230000008901 benefit Effects 0.000 title claims abstract description 29
- 238000000034 method Methods 0.000 title claims abstract description 25
- 230000000384 rearing effect Effects 0.000 title claims abstract description 15
- 230000009286 beneficial effect Effects 0.000 claims abstract description 73
- 230000012447 hatching Effects 0.000 claims abstract description 40
- 238000009395 breeding Methods 0.000 claims abstract description 14
- 230000001488 breeding effect Effects 0.000 claims abstract description 14
- 241000409991 Mythimna separata Species 0.000 claims description 55
- 235000013601 eggs Nutrition 0.000 claims description 40
- 241000819999 Nymphes Species 0.000 claims description 32
- 241000238631 Hexapoda Species 0.000 claims description 27
- 229920000742 Cotton Polymers 0.000 claims description 15
- 102000002322 Egg Proteins Human genes 0.000 claims description 12
- 108010000912 Egg Proteins Proteins 0.000 claims description 12
- 230000013011 mating Effects 0.000 claims description 12
- 210000004681 ovum Anatomy 0.000 claims description 12
- 235000013305 food Nutrition 0.000 claims description 9
- 239000004033 plastic Substances 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 238000005286 illumination Methods 0.000 claims description 7
- 238000004140 cleaning Methods 0.000 claims description 6
- 238000011534 incubation Methods 0.000 claims description 6
- 239000013589 supplement Substances 0.000 claims description 4
- 210000004700 fetal blood Anatomy 0.000 claims description 3
- 239000011521 glass Substances 0.000 claims description 3
- 235000016709 nutrition Nutrition 0.000 claims description 3
- 230000035764 nutrition Effects 0.000 claims description 3
- 230000004083 survival effect Effects 0.000 claims description 3
- 238000012549 training Methods 0.000 claims description 3
- 241000256844 Apis mellifera Species 0.000 claims 1
- 101150073877 egg-1 gene Proteins 0.000 claims 1
- 239000003292 glue Substances 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 238000011020 pilot scale process Methods 0.000 abstract description 2
- 238000011160 research Methods 0.000 abstract description 2
- 230000008569 process Effects 0.000 description 6
- 241000382353 Pupa Species 0.000 description 3
- 241000607479 Yersinia pestis Species 0.000 description 3
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- 235000019789 appetite Nutrition 0.000 description 2
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- 238000005516 engineering process Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000037406 food intake Effects 0.000 description 2
- 230000002787 reinforcement Effects 0.000 description 2
- 206010002091 Anaesthesia Diseases 0.000 description 1
- 241000283618 Arma chinensis Species 0.000 description 1
- 241001124134 Chrysomelidae Species 0.000 description 1
- 241000254173 Coleoptera Species 0.000 description 1
- 241000917122 Greenidea Species 0.000 description 1
- 241000255777 Lepidoptera Species 0.000 description 1
- 206010054949 Metaplasia Diseases 0.000 description 1
- 206010033799 Paralysis Diseases 0.000 description 1
- 241000320508 Pentatomidae Species 0.000 description 1
- 241000219000 Populus Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 230000037005 anaesthesia Effects 0.000 description 1
- 235000021405 artificial diet Nutrition 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
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- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000008451 emotion Effects 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
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Abstract
The invention discloses a kind of rearing method of scale benefit stinkbug, including following link, A. adult mate and oviposits;B, the hatching of pieces of an egg;C, 25 age nymphs are raised;D, the raising and release of adult.The present inventor is devoted for years to the research of the biological characteristics in beneficial stinkbug, predation and artificial feeding; large-scale breeding benefit stinkbug is realized by lab scale, pilot scale; by 2017; have been realized in producing per year beneficial stinkbug more than 1,000,000; using the large-scale cultivation not only efficiently, save the area, and cost is low, is easy to promote.
Description
Technical field
The present invention relates to a kind of rearing method of scale benefit stinkbug, belong to beneficial stinkbug artificial diet technique field.
Background technology
With popularizing for the ecological agriculture, insect bioremediation Technology is progressively promoted agricultural, various days
The breeding and culture for opposing insect are paid attention to by by people, and turn into a new industry.
Beneficial stinkbug (Arma chinensis Fallou) belongs to Semiptera Pentatomiddae, be a kind of predation scope it is wide, it is adaptable can
One of Important Natural Enemy insect for biological control, prey on the adult of more than the 40 kinds of agriculture and forestry injurious insect such as Lepidoptera and coleoptera, pupa,
Larva and ovum, most like to prey on slimacodiidae and the insect of leaf beetle section.Pest population quantity, which is increased, notable control action.Should
Agriculture and forestry injurious insect is prevented and treated with beneficial stinkbug, as long as selecting the suitable place of its ecological environment, optimum period continuously manually discharges 2~3 years, you can
Release area forms the relatively stable purpose for colonizing population and gradually being spread to its surrounding, reach sustainable control various pests.Benefit
Stinkbug is applied to preventing and treating agriculture and forestry injurious insect, and not only significant effect is lasting, and can largely reduce the amount of application of agricultural chemicals, both protects agricultural
Ecological environment, also comply with national green idea of development and realize the developing direction of pest and disease damage green prevention and control.
Beneficial stinkbug has a mouthpart as syringe, when catching and killing insect, mouthpart is pierced into other side's body, in mouthpart
The injection of one branch pipe(tube) makes the anesthesia liquid that other side paralyses, and another pipeline just sucks the body fluid of other side.Whether fierce and tough pine moth, also
It is that the slug moth of malicious hair all over is helplessly waited for death once meeting beneficial stinkbug, beneficial stinkbug flutters that appetite is very big, and a second instar larvae can solar eclipse poplar fan boat
15, moth ovum, adult can solar eclipse pine moth 15, appetite is greater than body weight several times.Due to the above-mentioned advantage of beneficial stinkbug, make
Insect is paid close attention to for new biological treating, beneficial stinkbug is of interest by those skilled in the art.But the existing report on beneficial stinkbug is in
Experimental stage, there has been no the complete skill scheme of scale metaplasia.
The content of the invention
The technical problem to be solved in the present invention is to provide a kind of scale benefit stinkbug for covering beneficial stinkbug artificial feeding links
Rearing method, can be with overcome the deficiencies in the prior art.
The technical scheme is that:The rearing method of scale benefit stinkbug includes following link
A. adult mate and oviposit, female male imago each one is merely placed in the mate and oviposit container of anti-escape in intelligent weather room,
And it is put into food and water in mate and oviposit container;The food cleared up once a day in mate and oviposit container in intelligent weather room
Residue and excrement, at the same supplements and water and collect spawning after pieces of an egg be used for artificial incubation;
B, the hatching of pieces of an egg, the pieces of an egg of collection are placed in the hatching culture dish for being covered with cotton paper, and keep hatching in culture dish
Tissue paper moistening, will hatching culture dish in growth cabinet, 24-27 DEG C of temperature, humidity RH55-65% are set, and illumination is with night
7-9h, 15-17h on daytime are advisable, and every morning takes out hatching culture dish and kept the skin wet, and 5-8 days pieces of an egg hatch 1 age nymph, 1 age
Nymph, which is raised 4-6 days, entered for 2 ages, entered for 3 ages within 6-7 days;.
C, 2-5 ages nymph is raised, and takes the insect cage of the anti-escape of 1-1.5m3 containers, 1500-2000 heads are put into insect cage
3-4 ages mythimna separata supplies beneficial stinkbug feeding, while the beneficial stinkbug in 3000-5000 head 2-3 ages is put into insect cage;The mythimna separata of timing cleaning daily
Frass, worm corpse, in the above mythimna separata benefit stinkbug raising ratio addition mythimna separata supply its feeding, be replaced and cleaned rearging cage one within 10 days or so
It is secondary, ensure clean environment in cage;
D, the raising and release of adult, 5 age nymphs about 8 days or so enters the adult stage, and the adult stage presses 3000 or so adult/cages,
1500 3-4 ages mythimna separata/cage ratios are put into cage and raised;The whole cage of adult benefit stinkbug for preparing release is put into intelligent weather
Room, temperature setting are 14-17 DEG C, humidity RH 60%;The beneficial stinkbug that will be discharged is taken out from intelligent climatic chamber, is sub-packed in dispensing container
Interior, injected volume 40-60 heads/mu is provided to need to launch region;The part imago breeding not discharged is used to lay eggs.
By imago breeding in intelligent weather room, 25-27 DEG C of temperature, humidity RH 60% are set, its adult mate and oviposit
Comprise the concrete steps that:
(1)Place paper;It is in broadside that the about long 6cm cut, wide 8cm general paper are folded into one end in wedge angle one end
" sector " after be put into preprepared a diameter of 8-10cm, high about 10-12cm mate and oviposit container, described mating
Spawning container is plastic cup, and the folded sheet supplies Adult worms producting eggs;
(2)Female male imago is placed;Using single pair mating method, female adult, the male worm of beneficial stinkbug adult are put into each mate and oviposit container
Each one, while each mate and oviposit container puts 3-4 age mythimna separatas 2-3 only, feeding when being laid eggs for beneficial stinkbug;
(3)Gauze is placed;Mate and oviposit container finish is covered in diameter about 13-7cm gauze, while with rubber band by gauze
It is fixed on mate and oviposit container;
(4)Cotton block is placed;6-8cm is put on gauze2One piece of the cotton of size, thickness about 3-5mm, every times/day of note on cotton
Enter 5% hydromel 6-8ml, ensure the nutrition needed for beneficial stinkbug adult and mythimna separata;
(5)The placement of mate and oviposit container;Mate and oviposit container is put on plastic pallet, each pallet puts 28 glasss, after putting well
Plastic pallet is put on the raising frame of intelligent climatic chamber;
(6)Cleaning and addition hydromel;Every morning 9-10 points add a hydromel, while clear up a mate and oviposit container
Interior worm corpse and frass residue, and regarding mythimna separata survival condition addition mythimna separata, be maintained at every glass of daily 2-3 only, put in intelligent weather room
After putting 7-9 days, beneficial stinkbug adult starts to lay eggs, and worm's ovum is collected when every morning 9-10 points add hydromel.
The hatching of described pieces of an egg comprises the following steps;
(1)By the worm's ovum 450-550 grains of collection, it is put in bottom and periphery and is covered with tissue paper, diameter 5-7cm, high 3-4cm hatching training
Support in ware, cover in hatching culture dish ware mouth yarn releasing net and fixed with rubber band;
(2)5% hydromel about 10-15ml is injected to the cotton block on culture dish gauze surface with syringe, while noted out of gauze hole
Enter to hatch the tissue paper inside culture dish, be advisable with bottom in ware without ponding, ensure that pieces of an egg hatching is humidity needed for this stage of larva,
Also the nymph feeding for just having hatched.
(3)Added with hydromel and the hatching culture dishes of pieces of an egg will be placed with it is put in growth cabinet, 26 DEG C of temperature, wet is set
RH60% is spent, illumination is advisable with evening 8h, 16h on daytime, and 10 points of every morning takes out culture dish and adds a hydromel, 6-8 days ovum
Block hatches 1 age nymph, and 1 age nymph, which is raised 4-6 days, entered for 2 ages, entered for 3 ages within about 5-7 days.
Described 2-5 ages nymph raising comprises the following steps;
(1)Insect cage prepares, and is ready for the insect cage of raising, and bottom is put yellow paper one and opened in cage, using be just covered in cage bottom portion as
Preferably, and by insect cage it is placed on foster worm frame, each shelf puts 6-9, cage;
(2)Mythimna separata is launched;3-4 age mythimna separatas are put into according to 1500-2000 heads/cage in cage, for beneficial stinkbug feeding;
(3)Beneficial stinkbug is placed, and the hatching culture dish equipped with the beneficial stinkbug nymph close to 3 ages is taken out, and will attach to hatch culture dish bottom
Beneficial stinkbug on portion's paper and covering surface gauze is put in rearging cage, quantity about 4000-5000 heads;
(4)Divide cage and put worm;Benefit stinkbug 3 age nymph entered for 4 ages within 6-8 days, entered for 5 ages after 5-7 days 4 age nymphs;With beneficial stinkbug
Growing needs a point cage, and for 3 age nymphs to put 4000-5000 heads, mythimna separata puts 1500-2000 heads;4 age nymphs put 3500- per cage
4000, mythimna separata puts 1800-2000 heads;5 age nymphs put 2800-3200 heads per cage, and mythimna separata puts 1400-1600 heads;Timing daily
Clear up frass, the worm corpse of mythimna separata;Its feeding is supplied in above mythimna separata and beneficial stinkbug raising ratio addition mythimna separata, is replaced and cleaned within 10 days or so
Rearging cage once, ensures clean environment in cage.
The 5 age benefit stinkbug nymph enters the adult stage in about 8 days or so, and the raising of adult and method for releasing are as follows:
(1)For high-density rearing in glasshouse, 5 age nymphs enter the adult stage, by 3000 or so adult/cages, 1500 3-4
Age mythimna separata/cage ratio is put into cage and raised;
(2)Prepare before release:Cord blood, by each issue of burst size, the whole cage adult in part is put into intelligent climatic chamber, temperature setting
For 15 DEG C, humidity RH 60%, the beneficial stinkbug pause feeding in this environment.
(3)Adult discharges and supply, prepares the beneficial stinkbug of release, it is taken out from intelligent climatic chamber, is sub-packed in homemade release
Put in sandbag, per packed 1000-2000 heads, be released to field control 20-40 mus, or loaded in self-control transport case, 50 are filled per box
Head, 1 mu is prevented and treated per box, there is provided to institute's release areas.
Compared with the prior art, the present inventor be devoted for years to the biological characteristics in beneficial stinkbug, predation and
The research of artificial feeding, large-scale breeding benefit stinkbug is realized by lab scale, pilot scale, by 2017, have been realized in producing benefit per year
Stinkbug more than 1,000,000.Patents in having generated many feeding process successively during experiment as long as five years, together
When applicant the various habits of beneficial stinkbug are found that in the breeding process of beneficial stinkbug, the large-scale breeding of beneficial stinkbug is carried out for habit
Distinguish:
1st, a very crucial link of artificial feeding be beneficial stinkbug spawning, and lay eggs key element for pairing and mating, this hair
The bright following features for being found that beneficial stinkbug:(1)The pairing of beneficial stinkbug is 1 female adult, 1 male worm, 1:1 pairing, typical monogamy
System, emotion is more single-minded, determines after mating object that all one's life is constant, in the environment of having other female adults and male worm, also only and
The companion of pairing carries out mating.(2)Beneficial stinkbug is completed the nymph stage, and last time decortication becomes after adult 3~4 days, in environment temperature
Pairing mating is proceeded by the case of degree and humidity are specific,(3)Diet and moisture supplement during pairing;(4)It is provided with
Specific container;These characteristics are applied to the artificial feeding of beneficial stinkbug, achieve good effect, pairing mating success rate is reachable
More than 95%, eggs on average amount 400 or so, the incubation rate of ovum is up to more than 90%.
2nd, in the hatching process to pieces of an egg, relevant feature is also summarized,(1)The environmental factors such as temperature, humidity, illumination,
Distinctive hatching culture dish structure;The nutrient at hatching initial stage causes the incubation rate of pieces of an egg most by the way of hydromel is mended up and down
High, best, its 6-8 days pieces of an egg hatch 1 age nymph, and 1 age nymph, which is raised 4-6 days, entered for 2 ages, entered for 3 ages within about 5-7 days, makes whole
Individual hatching process process is simple, is adapted to planningization breeding.
3rd, in the raising stage in 3-5 ages, it was found that be best suitable for the mythimna separata for serving as its food and facilitating artificial propagation to raise, together
When according to many experiments summarize optimal feeding ratio and beneficial stinkbug stable breeding amount, it is ensured that in the useful space health of beneficial stinkbug and
Its efficient rate of growth;
4th, in the stage of dispensing, the feed of beneficial stinkbug and its mobility is slowed down by effective temperature setting, avoid its growth too fast, and
Effective hand breeding, and facilitate encapsulation dispensing and its natural mating in field of the beneficial stinkbug in field, and drawn by testing inspection
Preferable beneficial stinkbug launches ratio.
Simultaneously for existing Candle-sticks stinkbugs, beneficial stinkbug is big with respect to Candle-sticks stinkbug egg laying amounts, each pair Adult worms producting eggs amount 300 or so, incubation rate
More than 90%, food ingestion is also larger, and every beneficial daily food ingestion of stinkbug adult is 2 or so more than Candle-sticks stinkbug.
Embodiment
The rearing method of scale benefit stinkbug includes following link
A. adult mate and oviposit, female male imago each one is merely placed in the mate and oviposit container of anti-escape in intelligent weather room,
And it is put into food and water in mate and oviposit container;The food cleared up once a day in mate and oviposit container in intelligent weather room
Residue and excrement, at the same supplements and water and collect spawning after pieces of an egg be used for artificial incubation;
B, the hatching of pieces of an egg, the pieces of an egg of collection are placed in the hatching culture dish for being covered with cotton paper, and keep hatching in culture dish
Tissue paper moistening, will hatching culture dish in growth cabinet, 24-27 DEG C of temperature, humidity RH55-65% are set, and illumination is with night
7-9h, 15-17h on daytime are advisable, and every morning takes out hatching culture dish and kept the skin wet, and 5-8 days pieces of an egg hatch 1 age nymph, 1 age
Nymph, which is raised 4-6 days, entered for 2 ages, entered for 3 ages within 6-7 days;.
C, 2-5 ages nymph is raised, and takes the insect cage of the anti-escape of 1-1.5m3 containers, 1500-2000 heads are put into insect cage
3-4 ages mythimna separata supplies beneficial stinkbug feeding, while the beneficial stinkbug in 3000-5000 head 2-3 ages is put into insect cage;The mythimna separata of timing cleaning daily
Frass, worm corpse, in the above mythimna separata benefit stinkbug raising ratio addition mythimna separata supply its feeding, be replaced and cleaned rearging cage one within 10 days or so
It is secondary, ensure clean environment in cage;
D, the raising and release of adult, 5 age nymphs about 8 days or so enters the adult stage, and the adult stage presses 3000 or so adult/cages,
1500 3-4 ages mythimna separata/cage ratios are put into cage and raised;The whole cage of adult benefit stinkbug for preparing release is put into intelligent weather
Room, temperature setting are 14-17 DEG C, humidity RH 60%;The beneficial stinkbug that will be discharged is taken out from intelligent climatic chamber, is sub-packed in dispensing container
Interior, injected volume 40-60 heads/mu is provided to need to launch region;The part imago breeding not discharged is used to lay eggs.
The artificial feeding details of each link is as follows:By imago breeding in intelligent weather room, 25-27 DEG C of temperature, wet is set
RH 60% is spent, described adult mate and oviposit comprises the concrete steps that
(1)Place paper;It is in broadside that the about long 6cm cut, wide 8cm general paper are folded into one end in wedge angle one end
" sector " after be put into preprepared a diameter of 8-10cm, high about 10-12cm mate and oviposit container, described mating
Spawning container is plastic cup, and the folded sheet supplies Adult worms producting eggs;
(2)Female male imago is placed;Using single pair mating method, female adult, the male worm of beneficial stinkbug adult are put into each mate and oviposit container
Each one, while each mate and oviposit container puts 3-4 ages mythimna separata 2 or pupa 2, feeding when being laid eggs for beneficial stinkbug;
(3)Gauze is placed;Mate and oviposit container finish is covered in diameter about 13-7cm gauze, while with rubber band by gauze
It is fixed on mate and oviposit container;
(4)Cotton block is placed;6-8cm is put on gauze2One piece of the cotton of size, thickness about 3-5mm, every times/day of note on cotton
Enter 5% hydromel 6-8ml, ensure the nutrition needed for beneficial stinkbug adult and mythimna separata;
(5)The placement of mate and oviposit container;Mate and oviposit container is put on plastic pallet, each pallet puts 28 glasss, after putting well
Plastic pallet is put on the raising frame of intelligent climatic chamber;
(6)Cleaning and addition hydromel;Every morning 9-10 points add a hydromel, while clear up a mate and oviposit container
Interior worm corpse, frass or pupa residue, and regarding mythimna separata survival condition addition mythimna separata, every glass of daily 2-3 is maintained at only, in intelligent weather room
After placing 7-9 days, beneficial stinkbug adult starts to lay eggs, and worm's ovum is collected when every morning 9-10 points add hydromel.
The hatching details of described pieces of an egg is as follows;
(1)By the worm's ovum 450-550 grains of collection, it is put in bottom and periphery and is covered with tissue paper, diameter 5-7cm, high 3-4cm hatching training
Support in ware, cover in hatching culture dish ware mouth yarn releasing net and fixed with rubber band;
(2)5% hydromel about 10-15ml is injected to the cotton block on culture dish gauze surface with syringe, while noted out of gauze hole
Enter to hatch the tissue paper inside culture dish, be advisable with bottom in ware without ponding, ensure that pieces of an egg hatching is humidity needed for this stage of larva,
Also the nymph feeding for just having hatched.
(3)Added with hydromel and the hatching culture dishes of pieces of an egg will be placed with it is put in growth cabinet, 26 DEG C of temperature, wet is set
RH60% is spent, illumination is advisable with evening 8h, 16h on daytime, and 10 points of every morning takes out culture dish and adds a hydromel, 6-8 days ovum
Block hatches 1 age nymph, and 1 age nymph, which is raised 4-6 days, entered for 2 ages, entered for 3 ages within about 5-7 days.
Applicant have also been devised a kind of new hatching culture dish simultaneously, and it includes bottom container, the container that reinforcement can absorb water
Wall, and its gauze made by reinforcement peripheral hardware water accepting layer rope yarn, it can thus save the process for supplementing hydromel one by one, more supernumerary segment
About human cost, reduce time of the pieces of an egg in incubator growth cabinet during hatching.
The detail of described 2-5 ages nymph raising is;
(1)Insect cage prepares, and is ready for the insect cage of raising, and bottom is put yellow paper one and opened in cage, using be just covered in cage bottom portion as
Preferably, and by insect cage it is placed on foster worm frame, each shelf puts 6-9, cage;
(2)Mythimna separata is launched;3-4 age mythimna separatas are put into according to 1500-2000 heads/cage in cage, for beneficial stinkbug feeding;
(3)Beneficial stinkbug is placed, and the hatching culture dish equipped with the beneficial stinkbug nymph close to 3 ages is taken out, and will attach to hatch culture dish bottom
Beneficial stinkbug on portion's paper and covering surface gauze is put in rearging cage, quantity about 4000-5000 heads;
(4)Divide cage and put worm;Benefit stinkbug 3 age nymph entered for 4 ages within 6-8 days, entered for 5 ages after 5-7 days 4 age nymphs;With beneficial stinkbug
Growing needs a point cage, and for 3 age nymphs to put 4000-5000 heads, mythimna separata puts 1500-2000 heads;4 age nymphs put 3500- per cage
4000, mythimna separata puts 1800-2000 heads;5 age nymphs put 2800-3200 heads per cage, and mythimna separata puts 1400-1600 heads;Timing daily
Clear up frass, the worm corpse of mythimna separata;Its feeding is supplied in above mythimna separata and beneficial stinkbug raising ratio addition mythimna separata, is replaced and cleaned within 10 days or so
Rearging cage once, ensures clean environment in cage.
The 5 age benefit stinkbug nymph enters the adult stage for about 8 days or so, and the raising of adult and release details are as follows:
(1)For high-density rearing in glasshouse, 5 age nymphs enter the adult stage, by 3000 or so adult/cages, 1500 3-4
Age mythimna separata/cage ratio is put into cage and raised;
(2)Prepare before release:Cord blood, by each issue of burst size, the whole cage adult in part is put into intelligent climatic chamber, temperature setting
For 15 DEG C, humidity RH 60%, the beneficial stinkbug pause feeding in this environment.
(3)Adult discharges and supply, prepares the beneficial stinkbug of release, it is taken out from intelligent climatic chamber, is sub-packed in homemade release
Put in sandbag, per packed 1000-2000 heads, be released to field control 20-40 mus, or loaded in self-control transport case, 50 are filled per box
Head, 1 mu is prevented and treated per box, there is provided to institute's release areas.
In order to avoid unnecessary repetition, the present invention no longer separately illustrates to various combinations of possible ways.But these simple changes
Type and combination should equally be considered as content disclosed in this invention, belong to protection scope of the present invention.
Claims (5)
- A kind of 1. rearing method of scale benefit stinkbug, it is characterised in that:This method includes following linkA. adult mate and oviposit, female male imago each one is merely placed in the mate and oviposit container of anti-escape in intelligent weather room, And it is put into food and water in mate and oviposit container;The food cleared up once a day in mate and oviposit container in intelligent weather room Residue and excrement, at the same supplements mythimna separata and water and collect spawning after pieces of an egg be used for artificial incubation;B, the hatching of pieces of an egg, the pieces of an egg of collection are placed in the hatching culture dish for being covered with cotton paper, and keep hatching in culture dish Tissue paper moistening, will hatching culture dish in growth cabinet, 24-27 DEG C of temperature, humidity RH55-65% are set, and illumination is with night 7-9h, 15-17h on daytime are advisable, and every morning takes out hatching culture dish and kept the skin wet, and 5-8 days pieces of an egg hatch 1 age nymph, 1 age Nymph, which is raised 4-6 days, entered for 2 ages, entered for 3 ages within 6-7 days;C, 2-5 ages nymph is raised, and takes the insect cage of the anti-escape of 1-1.5m3 containers, 1500-2000 heads 3-4 is put into insect cage Age mythimna separata supplies beneficial stinkbug feeding, while the beneficial stinkbug in 3000-5000 head 2-3 ages is put into insect cage;The mythimna separata of timing cleaning daily Frass, worm corpse, in the above mythimna separata benefit stinkbug raising ratio addition mythimna separata supply its feeding, be replaced and cleaned within 9-11 days rearging cage once, guarantor Hinder clean environment in cage;D, the raising and release of adult, 5 age nymphs 6-10 days enter the adult stage, and the adult stage presses 2800-3200 heads adult/cage, glue Worm is put into cage in 3-4 ages 1500/cage ratio and raised;The whole cage of adult benefit stinkbug for preparing release is put into intelligent weather Room, temperature setting are 14-17 DEG C, humidity RH 60%;The beneficial stinkbug that will be discharged is taken out from intelligent climatic chamber, is sub-packed in dispensing container Interior, injected volume 40-60 heads/mu is provided to need to launch region;The part imago breeding not discharged is used to lay eggs.
- 2. the rearing method of scale benefit stinkbug according to claim 1, it is characterised in that:By imago breeding in intelligence In climatic chamber, 25-27 DEG C of temperature, humidity RH 60% are set, described adult mate and oviposit comprises the concrete steps that(1)Place paper;The long 6cm cut, wide 8cm general paper are folded into one end in wedge angle one end in broadside It is put into after " sector " in preprepared a diameter of 8-10cm, a height of 10-12cm mate and oviposit container, described mating production Ovum container is plastic cup, and the folded sheet supplies Adult worms producting eggs;(2)Female male imago is placed;Using single pair mating method, female adult, the male worm of beneficial stinkbug adult are put into each mate and oviposit container Each one, while each mate and oviposit container puts 3-4 age mythimna separatas 2-3 only, feeding when being laid eggs for beneficial stinkbug;(3)Gauze is placed;Mate and oviposit container finish is covered in a diameter of 13-17cm gauze, while with rubber band by yarn Net is fixed on mate and oviposit container;(4)Cotton block is placed;6-8cm is put on gauze2One piece of the cotton of size, thick 3-5mm, inject 5% honeybee daily on cotton Mulse 6-8ml, ensure the nutrition needed for beneficial stinkbug adult and mythimna separata;(5)The placement of mate and oviposit container;Mate and oviposit container is put on plastic pallet, each pallet puts 28 glasss, after putting well Plastic pallet is put on the raising frame of intelligent climatic chamber;(6)Cleaning and addition hydromel;Every morning 9-10 points add a hydromel, while clear up a mate and oviposit container Interior worm corpse and frass residue, and regarding mythimna separata survival condition addition mythimna separata, be maintained at every glass of daily 2-3 only, put in intelligent weather room After putting 7-9 days, beneficial stinkbug adult starts to lay eggs, and worm's ovum is collected when every morning 9-10 points add hydromel.
- 3. the rearing method of scale benefit stinkbug according to claim 1, it is characterised in that:The hatching of described pieces of an egg Comprise the following steps;(1)By the worm's ovum 400-450 grains of collection, it is put in bottom and periphery and is covered with tissue paper, diameter 5-7cm, high 3-4cm hatching training Support in ware, covered with gauze in hatching culture dish ware mouth and fixed with rubber band;(2)5% hydromel about 10-15ml is injected to the cotton block on culture dish gauze surface with syringe, while noted out of gauze hole Enter to hatch the tissue paper inside culture dish, be advisable with bottom in ware without ponding, ensure that pieces of an egg hatching is humidity needed for this stage of larva, Also the nymph feeding for just having hatched;(3)Added with hydromel and the hatching culture dishes of pieces of an egg will be placed with it is put in growth cabinet, 26 DEG C of temperature, humidity is set RH60%, illumination are advisable with evening 8h, 16h on daytime, and 10 points of every morning takes out culture dish and adds a hydromel, 6-8 days pieces of an egg 1 age nymph is hatched, 1 age nymph, which is raised 4-6 days, entered for 2 ages, entered for 3 ages within about 5-7 days.
- 4. the rearing method of scale benefit stinkbug according to claim 1, it is characterised in that:Described 2-5 age nymphs Raising comprises the following steps;(1)Insect cage prepares, and is ready for the insect cage of raising, and bottom is put yellow paper one and opened in cage, using be just covered in cage bottom portion as Preferably, and by insect cage it is placed on foster worm frame, each shelf puts 6-9, cage;(2)Mythimna separata is launched;3-4 age mythimna separatas are put into according to 1500-2000 heads/cage in cage, for beneficial stinkbug feeding;(3)Beneficial stinkbug is placed, and the hatching culture dish equipped with the beneficial stinkbug nymph close to 3 ages is taken out, and will attach to hatch culture dish bottom Beneficial stinkbug on portion's paper and covering surface gauze is put in rearging cage, quantity about 4000-5000 heads;(4)Divide cage and put worm;Benefit stinkbug 3 age nymph entered for 4 ages within 6-8 days, entered for 5 ages after 5-7 days 4 age nymphs;With beneficial stinkbug Growing needs a point cage, and for 3 age nymphs to put 4000-5000 heads, mythimna separata puts 1500-2000 heads;4 age nymphs put 3500- per cage 4000, mythimna separata puts 1800-2000 heads;5 age nymphs put 2800-3200 heads per cage, and mythimna separata puts 1400-1600 heads;Timing daily Clear up frass, the worm corpse of mythimna separata;Its feeding is supplied in above mythimna separata and beneficial stinkbug raising ratio addition mythimna separata, is replaced and cleaned within 9-11 days feeding Foster cage once, ensures clean environment in cage.
- 5. the rearing method of scale benefit stinkbug according to claim 1, it is characterised in that:The 5 age benefit stinkbug nymph Enter the adult stage within 6-9 days, the raising of adult and method for releasing are as follows:(1)For high-density rearing in glasshouse, 5 age nymphs enter the adult stage, by 2800-3200 heads adult/cage, 1500 3- 4 age mythimna separatas/cage ratio is put into cage and raised;(2)Prepare before release:Cord blood, by each issue of burst size, the whole cage adult in part is put into intelligent climatic chamber, temperature setting For 15 DEG C, humidity RH 60%, the beneficial stinkbug pause feeding in this environment;(3)Adult discharges and supply, prepares the beneficial stinkbug of release, it is taken out from intelligent climatic chamber, and it is husky to be sub-packed in homemade release In bag, per packed 1000-2000 heads, field control 20-40 mus are released to, or loaded in self-control transport case, fill 50 per box, often Box prevents and treats 1 mu, there is provided to institute's release areas.
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108496900A (en) * | 2018-04-24 | 2018-09-07 | 云南农业大学 | Prevent the method for breeding and application method of Moringa Nao snout moth's larva Predacious functions |
CN109644947A (en) * | 2018-12-29 | 2019-04-19 | 广州瑞丰生物科技有限公司 | A kind of cultural method of Predacious function |
CN110384077A (en) * | 2019-08-30 | 2019-10-29 | 贵州省烟草公司六盘水市公司 | A kind of Candle-sticks stinkbug egg hatching method |
CN112042598A (en) * | 2020-07-28 | 2020-12-08 | 溧阳益植生物技术有限公司 | Complete set method for efficiently breeding harmonia axyridis |
CN113261538A (en) * | 2021-06-30 | 2021-08-17 | 中国热带农业科学院环境与植物保护研究所 | Artificial breeding method of predatory stinkbug natural enemy cissa major eye stinkbug |
CN114431194A (en) * | 2022-01-20 | 2022-05-06 | 中国农业科学院茶叶研究所 | Artificial breeding method of orius viridis |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20110078610A (en) * | 2009-12-31 | 2011-07-07 | 김수호 | Breeding artificial diet compositions for acherontia styx and mass-rearing method in a room condition of using the artificial diet |
CN105075992A (en) * | 2015-07-07 | 2015-11-25 | 华中农业大学 | Method for raising natural enemy insect southern minute pirate bug indoor in great deal and raising box |
CN205266667U (en) * | 2015-12-26 | 2016-06-01 | 广州清涟农业发展有限公司 | Benefit stinkbug subfamily insect rearing box |
CN106035245A (en) * | 2016-06-29 | 2016-10-26 | 安徽师范大学 | Method for breeding halyomorpha picus fabricius by using corns |
-
2017
- 2017-09-30 CN CN201710937580.0A patent/CN107410225A/en active Pending
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20110078610A (en) * | 2009-12-31 | 2011-07-07 | 김수호 | Breeding artificial diet compositions for acherontia styx and mass-rearing method in a room condition of using the artificial diet |
CN105075992A (en) * | 2015-07-07 | 2015-11-25 | 华中农业大学 | Method for raising natural enemy insect southern minute pirate bug indoor in great deal and raising box |
CN205266667U (en) * | 2015-12-26 | 2016-06-01 | 广州清涟农业发展有限公司 | Benefit stinkbug subfamily insect rearing box |
CN106035245A (en) * | 2016-06-29 | 2016-10-26 | 安徽师范大学 | Method for breeding halyomorpha picus fabricius by using corns |
Non-Patent Citations (2)
Title |
---|
严静君: "益蝽亚科的五种捕食蝽", 《森林病虫通讯》 * |
邹德玉: "取食无昆虫成分人工饲料蠋蝽的转录组研究及饲养成分分析", 《中国博士学位论文全文数据库 农业科技辑》 * |
Cited By (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108496900A (en) * | 2018-04-24 | 2018-09-07 | 云南农业大学 | Prevent the method for breeding and application method of Moringa Nao snout moth's larva Predacious functions |
CN108496900B (en) * | 2018-04-24 | 2020-11-24 | 云南农业大学 | Breeding method and using method for preventing and treating lygus tridentatus for moringa oleifera naotus |
CN109644947A (en) * | 2018-12-29 | 2019-04-19 | 广州瑞丰生物科技有限公司 | A kind of cultural method of Predacious function |
CN109644947B (en) * | 2018-12-29 | 2021-12-21 | 广州瑞丰生物科技有限公司 | Breeding method of lygus furcatus |
CN110384077A (en) * | 2019-08-30 | 2019-10-29 | 贵州省烟草公司六盘水市公司 | A kind of Candle-sticks stinkbug egg hatching method |
CN110384077B (en) * | 2019-08-30 | 2021-11-16 | 贵州省烟草公司六盘水市公司 | Lugworm egg hatching method |
CN112042598A (en) * | 2020-07-28 | 2020-12-08 | 溧阳益植生物技术有限公司 | Complete set method for efficiently breeding harmonia axyridis |
CN113261538A (en) * | 2021-06-30 | 2021-08-17 | 中国热带农业科学院环境与植物保护研究所 | Artificial breeding method of predatory stinkbug natural enemy cissa major eye stinkbug |
CN113261538B (en) * | 2021-06-30 | 2022-06-24 | 中国热带农业科学院环境与植物保护研究所 | Artificial breeding method of predatory stinkbug natural enemy cissa major eye stinkbug |
CN114431194A (en) * | 2022-01-20 | 2022-05-06 | 中国农业科学院茶叶研究所 | Artificial breeding method of orius viridis |
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