CN107384923B - 启动子pYLG及其在构建高产长链二元酸的热带假丝酵母中的应用 - Google Patents
启动子pYLG及其在构建高产长链二元酸的热带假丝酵母中的应用 Download PDFInfo
- Publication number
- CN107384923B CN107384923B CN201710645497.6A CN201710645497A CN107384923B CN 107384923 B CN107384923 B CN 107384923B CN 201710645497 A CN201710645497 A CN 201710645497A CN 107384923 B CN107384923 B CN 107384923B
- Authority
- CN
- China
- Prior art keywords
- pylg
- pcr
- kanr
- follows
- 30sec
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
- C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
- C12Y207/0103—Glycerol kinase (2.7.1.30)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Mycology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
Claims (5)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201710645497.6A CN107384923B (zh) | 2017-08-01 | 2017-08-01 | 启动子pYLG及其在构建高产长链二元酸的热带假丝酵母中的应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201710645497.6A CN107384923B (zh) | 2017-08-01 | 2017-08-01 | 启动子pYLG及其在构建高产长链二元酸的热带假丝酵母中的应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN107384923A CN107384923A (zh) | 2017-11-24 |
CN107384923B true CN107384923B (zh) | 2020-12-01 |
Family
ID=60344511
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201710645497.6A Active CN107384923B (zh) | 2017-08-01 | 2017-08-01 | 启动子pYLG及其在构建高产长链二元酸的热带假丝酵母中的应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN107384923B (zh) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109797165B (zh) * | 2018-07-21 | 2020-07-28 | 齐鲁工业大学 | 一种利用无痕编辑技术提高二元酸产量的方法 |
Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102839133A (zh) * | 2011-06-21 | 2012-12-26 | 上海凯赛生物技术研发中心有限公司 | 一种长链二元酸生产菌株及其应用 |
CN103080305A (zh) * | 2010-06-18 | 2013-05-01 | 丹麦科技大学 | 酵母由木质纤维素和甘油生产生物柴油 |
JP2014070030A (ja) * | 2012-09-28 | 2014-04-21 | Nippon Shokubai Co Ltd | 長鎖ジカルボン酸の製造方法 |
CN103805642A (zh) * | 2012-11-07 | 2014-05-21 | 中国石油化工股份有限公司 | 一种生产长链二元酸的发酵方法 |
CN105400796A (zh) * | 2015-12-28 | 2016-03-16 | 齐鲁工业大学 | 一种调控产长链二元酸的基因及其应用 |
CN106636156A (zh) * | 2016-12-26 | 2017-05-10 | 齐鲁工业大学 | 一种联产长链二元酸和1,3‑丙二醇的工程菌及其构建方法 |
CN106754979A (zh) * | 2016-12-26 | 2017-05-31 | 齐鲁工业大学 | 一种调控热带假丝酵母的长链脂肪酸转运的基因及其应用 |
-
2017
- 2017-08-01 CN CN201710645497.6A patent/CN107384923B/zh active Active
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103080305A (zh) * | 2010-06-18 | 2013-05-01 | 丹麦科技大学 | 酵母由木质纤维素和甘油生产生物柴油 |
CN102839133A (zh) * | 2011-06-21 | 2012-12-26 | 上海凯赛生物技术研发中心有限公司 | 一种长链二元酸生产菌株及其应用 |
JP2014070030A (ja) * | 2012-09-28 | 2014-04-21 | Nippon Shokubai Co Ltd | 長鎖ジカルボン酸の製造方法 |
CN103805642A (zh) * | 2012-11-07 | 2014-05-21 | 中国石油化工股份有限公司 | 一种生产长链二元酸的发酵方法 |
CN105400796A (zh) * | 2015-12-28 | 2016-03-16 | 齐鲁工业大学 | 一种调控产长链二元酸的基因及其应用 |
CN106636156A (zh) * | 2016-12-26 | 2017-05-10 | 齐鲁工业大学 | 一种联产长链二元酸和1,3‑丙二醇的工程菌及其构建方法 |
CN106754979A (zh) * | 2016-12-26 | 2017-05-31 | 齐鲁工业大学 | 一种调控热带假丝酵母的长链脂肪酸转运的基因及其应用 |
Non-Patent Citations (3)
Title |
---|
Accession no. CP017555.1;NCBI;《GenBank》;20161025;完整序列 * |
Development of mazF-based markerless genome editing system and metabolic pathway engineering in Candida tropicalis for producing long-chain dicarboxylic acids;Wang J.et al.;《J.IND.MICROBIOL.BIOTECHNOL.》;20180905;第45卷(第11期);971-981 * |
NCBI.Accession no. CP017555.1.《GenBank》.2016,完整序列. * |
Also Published As
Publication number | Publication date |
---|---|
CN107384923A (zh) | 2017-11-24 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN108753669B (zh) | 一种腺嘌呤生产菌株及其构建方法和应用 | |
CN105051181B (zh) | 2,3-丁二醇的生成能力增加的重组微生物及利用其的2,3-丁二醇的制备方法 | |
CN114874964A (zh) | 一种高产2′-岩藻糖基乳糖的重组大肠杆菌的构建方法及应用 | |
CN102965324B (zh) | 产(r, r)-2,3-丁二醇的基因工程菌及其构建方法和应用 | |
CN107384923B (zh) | 启动子pYLG及其在构建高产长链二元酸的热带假丝酵母中的应用 | |
CN104789586B (zh) | 大肠杆菌基因组整合载体、基因工程菌以及在生产木糖醇中的应用 | |
CN108103049B (zh) | 一种嗜热l-天冬酰胺酶突变体及其筛选和发酵方法 | |
CN115058374B (zh) | 一种利用丙酮酸合成乙偶姻的重组运动发酵单胞菌及其构建方法与应用 | |
CN111334459A (zh) | 一种提高1,3-丙二醇产量的克雷伯氏工程菌构建方法及应用 | |
CN111500479B (zh) | 一种非甲醇诱导双启动子毕赤酵母工程菌的构建及其应用 | |
CN106636156B (zh) | 一种联产长链二元酸和1,3-丙二醇的工程菌及其构建方法 | |
CN107119003B (zh) | 一种利用葡聚糖合成3-羟基丙酸的重组菌及其构建方法和应用 | |
CN114854659A (zh) | 一种麦角硫因生产工艺及其应用 | |
CN116024150A (zh) | 一种生产乙偶姻基因工程菌株及其构建方法与应用 | |
CN116676243A (zh) | 产2'-岩藻糖基乳糖的重组大肠杆菌的构建方法及其应用 | |
CN114561416A (zh) | 利用电能固定co2及合成异丙醇的工程菌及构建方法 | |
CN104845995A (zh) | 一种动态调控苏氨酸外排转运蛋白基因表达生产l-苏氨酸的方法 | |
CN109370969B (zh) | 一种重组克雷伯氏杆菌在制备1,3-丙二醇中的应用 | |
CN103834680A (zh) | 一种用农杆菌介导对裂壶藻进行基因转化的方法 | |
CN101892228B (zh) | 一种高丙烯酰胺和丙烯腈耐受性产腈水合酶工程菌及应用 | |
CN113186217A (zh) | 适用于聚球藻UTEX 2973的Neu5Ac-NanR诱导系统及构建方法与用途 | |
CN106801063B (zh) | 一种形态改变的工程大肠杆菌的构建方法、工程大肠杆菌及应用 | |
CN110669774A (zh) | 一种β-甘露聚糖酶密码子优化序列及提高毕赤酵母工程菌表达水平的方法 | |
CN113913451B (zh) | 一株产肌醇的巴斯德毕赤酵母工程菌的构建方法 | |
CN114875003B (zh) | 一种短链脱氢酶的突变体、编码基因及编码基因获得方法、突变体的应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
CB03 | Change of inventor or designer information | ||
CB03 | Change of inventor or designer information |
Inventor after: Wang Junqing Inventor after: Xiu Xiang Inventor after: Peng Jian Inventor after: Wang Ruiming Inventor after: Xue Le Inventor after: Wang Tengfei Inventor after: Su Jing Inventor after: Yang Xiaohui Inventor before: Wang Junqing Inventor before: Peng Jian Inventor before: Wang Ruiming Inventor before: Xiu Xiang Inventor before: Xue Le Inventor before: Wang Tengfei Inventor before: Su Jing Inventor before: Yang Xiaohui |
|
GR01 | Patent grant | ||
GR01 | Patent grant |