CN107258447B - Asexual propagation method of mango fine variety for greening - Google Patents

Asexual propagation method of mango fine variety for greening Download PDF

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CN107258447B
CN107258447B CN201710599640.2A CN201710599640A CN107258447B CN 107258447 B CN107258447 B CN 107258447B CN 201710599640 A CN201710599640 A CN 201710599640A CN 107258447 B CN107258447 B CN 107258447B
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cutting
mango
cuttage
greening
solution
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CN107258447A (en
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韦璐阳
邓有展
蒋济刚
覃剑锋
蓝唯
杨秀娟
吴静娜
王运儒
董利军
农耀京
李进华
钟耀才
钟程
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Guangxi Subtropical Crops Research Institute
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005Cultivation methods
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C21/00Methods of fertilising, sowing or planting
    • A01C21/005Following a specific plan, e.g. pattern
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/06Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/10Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/36Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
    • A01N43/38Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/16Heavy metals; Compounds thereof

Abstract

The invention discloses a vegetative propagation method of a mango fine variety for greening, which comprises the procedures of stock plant selection, propagation material acquisition, cutting shoot collection, cutting shoot pruning, rooting solution preparation, cuttage and management after cuttage, wherein the mango fine variety for greening is selected as a cutting shoot stock plant, the cutting shoot stock plant is subjected to girdling to obtain strong and disease and insect pest-free bud strips, the bud strips are collected, pruned, soaked in rooting solution and subjected to cuttage, and then mango cutting seedlings for greening are obtained through management after cuttage. The method screens varieties according to the breeding requirements of garden tree species, screens out excellent mango varieties suitable for landscaping cultivation, changes the traditional purposeless disordered afforesting mango breeding cultivation habit, improves the commodity value of afforesting mangoes, and meets the market demand.

Description

Asexual propagation method of mango fine variety for greening
Technical Field
The invention belongs to the technical field of plant asexual propagation, and relates to an asexual propagation method of a mango fine variety for greening.
Background
Mango (A)Mangifera indica L.) Is an evergreen arbor of the genus mango of the family Anacardiaceae, native to the border between Asamu, India and Burma, widely cultivated in southern areas of China, and is one of famous tropical fruits. The mango tree has the advantages of high and large crown, dense branches and leaves, dark green leaves, long service life of hundreds of years, wide leaf surface, obvious dust blocking, filtering and adsorbing effects, and remarkable functions of urban landscaping and air purification. However, at present, mango breeding is mainly carried out as an index required by fruit trees, the key selection object is a high-yield and high-quality variety of fruits, and the mango variety used as the fruit tree is mostly not suitable for landscaping application, so that the mango variety screening needs to be carried out according to the breeding requirement of garden tree species for developing greening. On-sieveThe method has the advantages that asexual propagation seedling raising is carried out on the basis of selecting excellent greening mangos, so that offspring can maintain the hereditary shape of the parent body and simultaneously expand the seedling propagation mode, the propagation speed is accelerated, a large number of high-quality seedlings with the same hereditary shape can be cultivated in a short time, the value of commercial seedlings is improved, the market demand is met, and the seedling guarantee is provided for the industrial development of greening mangos.
Disclosure of Invention
The invention provides a vegetative propagation method of a good variety of mango for greening, which is stable in heredity and can cultivate a large amount of high-quality healthy greening seedlings in a short period, aiming at the problem that no mango variety suitable for being used as greening seedlings exists in the current greening seedling market and the innovated research of the vegetative propagation seedling raising technology of mango for greening is carried out on the basis of variety screening.
The technical scheme of the invention is as follows:
a vegetative propagation method of mango fine varieties for greening comprises the procedures of stock plant selection, propagation material acquisition, cutting collection, cutting pruning, rooting solution preparation, cuttage and management after cuttage, wherein the mango fine varieties for greening are selected as cutting stock plants, the cutting stock plants are subjected to girdling to obtain strong and disease and insect pest-free bud strips which grow to 40-60 cm and are free of diseases and insect pests, the bud strips are collected, pruned, soaked in rooting solution and subjected to cuttage, and mango cutting seedlings for greening are obtained through management after cuttage; the main operation steps are as follows:
(1) selecting a stock plant: selecting a greening mango fine variety which grows for 5-8 years by seed propagation, has a tree height annual growth of more than 2.0m and a rice diameter annual growth of more than 1.5cm as a scion stock plant;
(2) obtaining of propagation materials: in spring with sufficient rainwater and rising air temperature, performing girdling on the position, about 30cm away from the ground, of the trunk of the scion stock plant, girdling 2/3 of the trunk circumference, with the width of 5-6 cm and the depth of phloem, and spraying 1.0-2.0 mg.L of concentration on the girdling position-1Cytokinin 6-BA to promote germination;
(3) and (3) cutting collection: collecting the sprout strips which grow to 40-60 cm in 3-10 months and are robust and free of diseases and insect pests and are obtained in the step (2) as cutting cuttings; the diameter of the cutting slips is 0.3-0.8 cm, the length is 8-15 cm, and 2-4 axillary buds are reserved;
(4) cutting and pruning: the morphological upper end of the cutting shoot is cut flatly, and the morphological lower end is cut obliquely at an angle of 45 degrees; cutting off partial leaves of the cutting slips, and only leaving 2-4 leaves at the top end;
(5) preparing a rooting solution: firstly, 37.3 mg.L of the medicine is used-1Na of (2)2EDTA and 27.8 mg.L-1FeSO of (2)4•7H2O chelating to obtain mixed solution of chelated iron, and adding 200-500 mg.L of the mixed solution of chelated iron-1Indole butyric acid and 100-300 mg.L-1The naphthylacetic acid is prepared into rooting liquid;
(6) cuttage: disinfecting the seedling culture substrate with a potassium permanganate solution with the volume concentration of 0.2-0.5% 2 days before cuttage, and leaching the seedling culture substrate with tap water one day before cuttage to remove potassium permanganate; soaking the base parts of the cutting slips in a rooting solution for 8-10 h, and then inserting the cutting slips into a seedling culture container filled with a seedling culture medium, wherein the cutting depth is 3-4 cm;
(7) managing after cuttage: immediately drenching water once after cuttage, covering a film, keeping the air humidity above 95%, avoiding high temperature and strong illumination, pulling a sunshade net with a shading rate of 80% at a time interval with strong sunlight at 10 am to 4 pm, and opening the film after cuttage for 7 days; spraying nutrient solution on the leaf surface in the 7 th day after cuttage, and spraying the nutrient solution once every 5 days for 3-5 times in total until the root is rooted; meanwhile, the seedling raising substrate is ensured to be wet, and finally, mango cutting seedlings for greening are obtained.
The fine variety of mango tree for greening in the step (1) has the advantages that the tree trunk is straight and round, does not diverge, does not form a pocket, does not twist and does not have tree knots; the tree trunk is complete, the crown is large, and the growth vigor is vigorous; the tree is healthy, has no diseases, insect pests and wind break; the height of the branch point of the 5-year-old tree is more than 2.5 m.
The seedling raising substrate in the step (6) is prepared from red subsoil and coconut coir according to the volume ratio of 3: 7.
The seedling raising container in the step (6) is a non-woven fabric seedling raising bag with the diameter of 10cm and the height of 12 cm.
The above steps(7) The nutrient solution is prepared by using 37.3 mg.L-1Na of (2)2EDTA and 27.8 mg. L-1FeSO of (2)4·7H2Chelating the iron into a chelated iron mixed solution, and adding 100-300 mg.L into the chelated iron mixed solution-1The indoleacetic acid is prepared into nutrient solution.
Compared with the prior art, the invention has the advantages and beneficial effects as follows:
1. the method screens varieties according to the breeding requirements of garden tree species, screens out excellent mango varieties suitable for landscaping cultivation, changes the traditional purposeless disordered afforesting mango breeding cultivation habit, improves the commodity value of afforesting mangoes, and meets the market demand.
2. The invention carries out seedling cultivation by a cutting asexual propagation mode, better maintains the excellent hereditary character of the greening mango variety, can obtain a large amount of bud strips by carrying out girdling on the stock plant, can cultivate seedlings with consistent growth, is convenient for seedling cultivation management, is beneficial to batch commercial seedlings to come out of the nursery, and has better economic benefit, social benefit and ecological benefit.
3. The method carries out hormone treatment and matrix selection according to the growth characteristics of mango trees, uses certain growth regulator types and concentration treatment materials, is convenient to obtain materials, easy to prepare, simple and convenient to operate, less in material consumption, low in cost and high in propagation speed.
Detailed Description
The present invention is further illustrated by the following specific examples.
Example 1:
selecting 5-6 years of growth on the mango suitable place Guangxi, wherein the growth is propagated by seeds, the tree height year growth is more than 2.0m, and the rice diameter year growth is more than 1.5 cm; the trunk is straight and round, does not have a fork, is not wrapped, has no distortion and has no tree tumor; the tree trunk is complete, the crown is large, and the growth vigor is vigorous; the tree is healthy, has no diseases, insect pests and wind break; a mango fine variety for greening with the branch point height of more than 2.5m of a 5-year-old tree is used as a scion stock plant.
In spring with sufficient rainwater and rising air temperature, performing girdling on the position, about 30cm away from the ground, of the trunk of the scion stock plant, wherein the trunk is girdled to 2/3 of the circumference and 5-6 of the widthcm, deep to phloem, and spraying at the girdling position with a concentration of 1.0 mg.L-1Cytokinin 6-BA to promote germination.
Collecting the buds which grow to 40-60 cm after girdling in 3-6 months, wherein the buds grow robustly and are free of diseases and insect pests and serve as cutting cuttings; the diameter of the cutting slips is 0.3-0.5 cm, the length is 8-10 cm, and 2-4 axillary buds are reserved. The morphological upper end of the cutting shoot is cut flatly, and the morphological lower end is cut obliquely at an angle of 45 degrees; and (4) cutting off partial leaves of the cutting slips, and only leaving 2-4 leaves at the top end.
Firstly, 37.3 mg.L of the medicine is used-1Na of (2)2EDTA and 27.8 mg.L-1FeSO of (2)4•7H2Chelating the iron into a chelate iron mixed solution, and adding 200 mg.L of the chelate iron mixed solution-1Indole butyric acid and 100 mg.L-1The naphthylacetic acid is prepared into rooting liquid.
Mixing red heart soil and coconut coir at a volume ratio of 3: 7 to obtain seedling substrate, and packaging into non-woven seedling bags with diameter of 10cm and height of 12 cm. Sterilizing the seedling culture substrate by using a potassium permanganate solution with the volume concentration of 0.2% 2 days before cuttage, and leaching the seedling culture substrate by using tap water one day before cuttage to flush away potassium permanganate; and (3) soaking the base parts of the cutting slips in a rooting solution for 8 hours, and then inserting the cutting slips into a seedling raising container filled with a seedling raising substrate, wherein the cutting depth is 3-4 cm.
Immediately drenching water once after cuttage, covering a film, keeping the air humidity above 95%, avoiding high temperature and strong illumination, pulling a sunshade net with a shading rate of 80% at a time interval with strong sunlight at 10 am to 4 pm, and opening the film after cuttage for 7 days; spraying a nutrient solution on the leaf surface of the leaf surface in the 7 th day after cuttage, wherein the nutrient solution is prepared by firstly using 37.3 mg.L-1Na of (2)2EDTA and 27.8 mg. L-1FeSO of (2)4·7H2Chelating the iron into a mixed solution of chelated iron, and adding 100 mg.L into the mixed solution of chelated iron-1Is prepared from indoleacetic acid. Spraying the nutrient solution every 5 days for 3-5 times until the root is rooted; meanwhile, if the seedling raising substrate is ensured to be wet, adventitious roots can be induced at the base of the cutting slips after 45 days, and finally, mango cutting seedlings for greening are obtained.
Example 2:
selecting 6-7 years of growth on the Guangdong suitable for the growth of the mango seeds, wherein the tree height annual growth is more than 2.0m, and the rice diameter annual growth is more than 1.5 cm; the trunk is straight and round, does not have a fork, is not wrapped, has no distortion and has no tree tumor; the tree trunk is complete, the crown is large, and the growth vigor is vigorous; the tree is healthy, has no diseases, insect pests and wind break; a mango fine variety for greening with the branch point height of more than 2.5m of a 5-year-old tree is used as a scion stock plant.
In spring with sufficient rainwater and rising air temperature, performing girdling on the position, about 30cm away from the ground, of the trunk of the scion stock plant, girdling 2/3 of the trunk circumference, with the width of 5-6 cm and the depth of phloem, and spraying 1.5 mg.L of concentration on the girdling position-1Cytokinin 6-BA to promote germination.
Collecting the buds which grow to 40-60 cm after girdling in 6-8 months, wherein the buds grow robustly and are free of diseases and insect pests and are used as cutting cuttings; the diameter of the cutting slips is 0.5-0.6 cm, the length is 10-12 cm, and 2-4 axillary buds are reserved. The morphological upper end of the cutting shoot is cut flatly, and the morphological lower end is cut obliquely at an angle of 45 degrees; and (4) cutting off partial leaves of the cutting slips, and only leaving 2-4 leaves at the top end.
Firstly, 37.3 mg.L of the medicine is used-1Na of (2)2EDTA and 27.8 mg.L-1FeSO of (2)4•7H2Chelating iron mixed solution by chelating O, and adding 300 mg.L into the chelated iron mixed solution-1Indole butyric acid and 200 mg.L-1The naphthylacetic acid is prepared into rooting liquid.
Mixing red heart soil and coconut coir at a volume ratio of 3: 7 to obtain seedling substrate, and packaging into non-woven seedling bags with diameter of 10cm and height of 12 cm. Sterilizing the seedling culture substrate by using a potassium permanganate solution with the volume concentration of 0.3% 2 days before cuttage, and leaching the seedling culture substrate by using tap water one day before cuttage to flush away potassium permanganate; and (3) soaking the base parts of the cutting slips in a rooting solution for 9h, and then inserting the cutting slips into a seedling raising container filled with a seedling raising substrate, wherein the cutting depth is 3-4 cm.
Immediately drenching water once after cuttage, covering with a film, keeping air humidity above 95%, avoiding high temperature and strong illumination, pulling a sunshade net with shading rate of 80% at the time of strong sunlight at 10 am-4 pm, and opening after cuttage for 7 daysA film; spraying a nutrient solution on the leaf surface of the leaf surface in the 7 th day after cuttage, wherein the nutrient solution is prepared by firstly using 37.3 mg.L-1Na of (2)2EDTA and 27.8 mg. L-1FeSO of (2)4·7H2Chelating the iron into a chelate iron mixed solution, and adding 200 mg.L into the chelate iron mixed solution-1Is prepared from indoleacetic acid. Spraying the nutrient solution every 5 days for 3-5 times until the root is rooted; meanwhile, if the seedling raising substrate is ensured to be wet, adventitious roots can be induced at the base of the cutting slips after 45 days, and finally, mango cutting seedlings for greening are obtained.
Example 3:
selecting 7-8 years of growth on the mango suitable place Hainan by seed propagation, wherein the tree height annual growth is more than 2.0m, and the rice diameter annual growth is more than 1.5 cm; the trunk is straight and round, does not have a fork, is not wrapped, has no distortion and has no tree tumor; the tree trunk is complete, the crown is large, and the growth vigor is vigorous; the tree is healthy, has no diseases, insect pests and wind break; a mango fine variety for greening with the branch point height of more than 2.5m of a 5-year-old tree is used as a scion stock plant.
In spring with sufficient rainwater and rising air temperature, performing girdling on the position, about 30cm away from the ground, of the trunk of the scion stock plant, girdling 2/3 of the trunk circumference, with the width of 5-6 cm and the depth of the trunk to the phloem, and spraying 2.0 mg.L of solution at the girdling position-1Cytokinin 6-BA to promote germination.
Collecting the buds which grow to 40-60 cm after girdling in 9-10 months, wherein the buds grow robustly and are free of diseases and insect pests and serve as cutting cuttings; the diameter of the cutting slips is 0.6-0.8 cm, the length is 12-15 cm, and 2-4 axillary buds are reserved. The morphological upper end of the cutting shoot is cut flatly, and the morphological lower end is cut obliquely at an angle of 45 degrees; and (4) cutting off partial leaves of the cutting slips, and only leaving 2-4 leaves at the top end.
Firstly, 37.3 mg.L of the medicine is used-1Na of (2)2EDTA and 27.8 mg.L-1FeSO of (2)4•7H2Chelating the iron into a chelate iron mixed solution, and adding 500 mg.L of the chelate iron mixed solution-1Indole butyric acid of (1) and 300 mg.L-1The naphthylacetic acid is prepared into rooting liquid.
Mixing red heart soil and coconut coir at a volume ratio of 3: 7 to obtain seedling substrate, and packaging into non-woven seedling bags with diameter of 10cm and height of 12 cm. Sterilizing the seedling culture substrate by using a potassium permanganate solution with the volume concentration of 0.5% 2 days before cuttage, and leaching the seedling culture substrate by using tap water one day before cuttage to flush away potassium permanganate; and (3) soaking the base parts of the cutting slips in a rooting solution for 10 hours, and then inserting the cutting slips into a seedling raising container filled with a seedling raising substrate, wherein the cutting depth is 3-4 cm.
Immediately drenching water once after cuttage, covering a film, keeping the air humidity above 95%, avoiding high temperature and strong illumination, pulling a sunshade net with a shading rate of 80% at a time interval with strong sunlight at 10 am to 4 pm, and opening the film after cuttage for 7 days; spraying a nutrient solution on the leaf surface of the leaf surface in the 7 th day after cuttage, wherein the nutrient solution is prepared by firstly using 37.3 mg.L-1Na of (2)2EDTA and 27.8 mg. L-1FeSO of (2)4·7H2Chelating the iron into a chelate iron mixed solution, and adding 300 mg.L into the chelate iron mixed solution-1Is prepared from indoleacetic acid. Spraying the nutrient solution every 5 days for 3-5 times until the root is rooted; meanwhile, if the seedling raising substrate is ensured to be wet, adventitious roots can be induced at the base of the cutting slips after 45 days, and finally, mango cutting seedlings for greening are obtained.

Claims (1)

1. A vegetative propagation method of a greening mango fine variety is characterized by comprising the following steps: selecting a good mango variety for greening as a cutting stock plant, performing girdling on the cutting stock plant to obtain sprout strips which grow to 40-60 cm and are robust and free of plant diseases and insect pests, collecting and trimming the sprout strips, soaking the sprout strips in a rooting solution for cuttage, and then performing management after cuttage to obtain mango cutting seedlings for greening; the main operation steps are as follows:
(1) selecting a stock plant: selecting a greening mango fine variety which grows for 5-8 years, grows for more than 2.0m in tree height year and grows for more than 1.5cm in rice diameter year and is propagated by seeds from Guangxi, Guangdong or Hainan of mango suitable places as a scion stock plant;
the tree trunk of the mango fine variety for greening is straight and round, does not have a fork, a pocket, a twist and a tree tumor; the tree trunk is complete, the crown is large, and the growth vigor is vigorous; the tree is healthy, has no diseases, insect pests and wind break; the height of the branch point of the 5-year-old tree is more than 2.5 m;
(2) obtaining of propagation materials: in spring with sufficient rainwater and rising air temperature, performing girdling on the position, about 30cm away from the ground, of the trunk of the scion stock plant, girdling 2/3 of the trunk circumference, with the width of 5-6 cm and the depth of phloem, and spraying 1.0-2.0 mg.L of concentration on the girdling position-1Cytokinin 6-BA to promote germination;
(3) and (3) cutting collection: collecting the sprout strips which grow to 40-60 cm in 3-10 months and are robust and free of diseases and insect pests and are obtained in the step (2) as cutting cuttings; the diameter of the cutting slips is 0.3-0.8 cm, the length is 8-15 cm, and 2-4 axillary buds are reserved;
(4) cutting and pruning: the morphological upper end of the cutting shoot is cut flatly, and the morphological lower end is cut obliquely at an angle of 45 degrees; cutting off partial leaves of the cutting slips, and only leaving 2-4 leaves at the top end;
(5) preparing a rooting solution: firstly, 37.3 mg.L of the medicine is used-1Na of (2)2EDTA and 27.8 mg.L-1FeSO of (2)4•7H2Chelating O to obtain mixed iron chelate solution, adding 200-500 mg.L-1 indolebutyric acid and 100-300 mg.L to the mixed iron chelate solution-1The naphthylacetic acid is prepared into rooting liquid;
(6) cuttage: disinfecting the seedling culture substrate with a potassium permanganate solution with the volume concentration of 0.2-0.5% 2 days before cuttage, and leaching the seedling culture substrate with tap water one day before cuttage to remove potassium permanganate; soaking the base parts of the cutting slips in a rooting solution for 8-10 h, and then inserting the cutting slips into a seedling culture container filled with a seedling culture medium, wherein the cutting depth is 3-4 cm; the seedling culture substrate is prepared from red heart soil and coconut coir according to the volume ratio of 3: 7; the seedling raising container is a non-woven fabric seedling raising bag with the diameter of 10cm and the height of 12 cm;
(7) managing after cuttage: immediately drenching water once after cuttage, covering a film, keeping the air humidity above 95%, avoiding high temperature and strong illumination, pulling a sunshade net with a shading rate of 80% at a time interval with strong sunlight at 10 am to 4 pm, and opening the film after cuttage for 7 days; spraying nutrient solution on the leaf surface in the 7 th day after cuttage, and spraying the nutrient solution once every 5 days for 3-5 times in total until the root is rooted; meanwhile, ensuring the seedling culture substrate to be moist, and finally obtaining mango cutting seedlings for greening;
the nutrient solution is prepared by using 37.3 mg.L-1Na of (2)2EDTA and 27.8 mg. L-1 of FeSO4·7H2Chelating the iron into a chelated iron mixed solution, and adding 100-300 mg.L into the chelated iron mixed solution-1The indoleacetic acid is prepared into nutrient solution.
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