CN105746295A - Quick cutting rooting method of grape - Google Patents
Quick cutting rooting method of grape Download PDFInfo
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- CN105746295A CN105746295A CN201610208814.3A CN201610208814A CN105746295A CN 105746295 A CN105746295 A CN 105746295A CN 201610208814 A CN201610208814 A CN 201610208814A CN 105746295 A CN105746295 A CN 105746295A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/02—Cultivation of hops or vines
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/029—Receptacles for seedlings
- A01G9/0299—Handling or transporting of soil blocks or seedlings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/36—Rutaceae [Rue family], e.g. lime, orange, lemon, corktree or pricklyash
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Abstract
The invention discloses a quick cutting rooting method of grape, comprising the following steps: (1), preparing cuttings, (2), preparing a cutting field, (3), preparing a rooting liquid, (4), treating the cuttings and performing cuttage, and (5), managing after cuttage. The method of the invention has simple and standardized operations, synergy of the operations provides higher than 98% of a stable cutting survival rate of grape, grown cutting seedlings has high rooting quantity, growing time is short, and the seedlings grow vigor and have high environmental stress resistance.
Description
Technical field
The invention belongs to field of planting, be specifically related to a kind of method that Fructus Vitis viniferae rapid cuttage is taken root.
Background technology
Fructus Vitis viniferae is the main fruit trees seeds of China, its fruit can be eaten raw, makes wine, juice processed and system dry etc., the deep welcome by consumer.The breeding of Fructus Vitis viniferae has the method for growing directly from seeds, cuttage, overhead layering method and grafting, cuttage is because of simple, the advantage such as growing-seedling period is short, reproduction speed is limited soon, not by seeds, breeding coefficient is higher, cost is low, the good characteristic that can keep elite stand, and is widely adopted.Application recently as the synthetic of plant growth regulator and auto spraying facility, its development is maked rapid progress especially, but following Problems existing is also very serious, especially plant growth regulator abuse in cottage propagation make produce in lose very big, the root-inducing powder occurred in the market just has more than hundreds of kind, most rooting efficiencies are very micro-, not only increase planting cost, and the taking root liquid after using deals with improperly and can cause serious environmental hazard, in addition, the seedling that tradition cuttage mode is cultivated still shows relatively low relative to the growing way in grafting later stage and survival rate, its growth characteristics still need to further raising.Additionally, the sprouting of more difficult control eye in normal cutting propagation method, often occur that eye crosses the problem that premature eruption reduces survival rate with root growth competition for nutrients.
Summary of the invention
In order to solve the problems referred to above, the invention provides a kind of method that Fructus Vitis viniferae rapid cuttage is taken root, have cuttage survival rate height, growing-seedling period is short, the feature that cutting seedling quality is high.
The present invention is achieved through the following technical solutions:
A kind of method that Fructus Vitis viniferae rapid cuttage is taken root, comprises the steps:
(1) prepared by cuttage bar: selects Fructus Vitis viniferae semi-lignified annotinous branch, is cut into the cutting that length is 7 ~ 9cm, and lower end is cut into the shape of a hoof, upper end straight snips, and each cutting retains 5 ~ 7 eyes；
(2) cuttage ground preparation: by fluvial sand, Vermiculitum, turfy soil is by weight mix homogeneously after 1:1:2 preparation, and carry out disinfection by disinfectant, then paved one-tenth length, width and height are the cuboid cuttage ground of 5m × 6m × 0.4m, described disinfectant is made up of the material of following weight portion: 10 ~ 15 portions of Radix Glycyrrhizaes, 8 ~ 10 parts of Herba Salviae Chinensiss, 5 ~ 10 parts of Flos Celosiae Cristataes, 6 ~ 8 parts of reed Ba Zi, 12 ~ 17 parts of Melaleuca Alternifolia leaveves, 5 ~ 8 parts of citron leaves, 3 ~ 5 portions of Rhizoma Menispermis, 3 ~ 5 parts of useful mycopowder, 1 ~ 2 part of muscardine, 5 ~ 8 parts of chitins, 3 ~ 5 parts of attapulgites, 4 ~ 7 parts of gelatin, 10 ~ 12 parts of starch, 20 ~ 25 parts of rice husks；
null(3) prepared by taking root liquid: weigh the material of following weight portion: 0.3 ~ 0.7 part of IBA root-growing agent、60 ~ 80 parts of amino-acid chelates、20 ~ 25 parts of compound vitamines、10 ~ 15 parts of Mel、2 ~ 3 parts of gentiobioses、2 ~ 4 parts of brown alga oligoses、5 ~ 10 parts of nitrogen、6 ~ 8 parts of phosphorus、5 ~ 8 parts of potassium、3 ~ 5 parts of Caulis Miscanthis floriduli extracts、2 ~ 3 parts of dehydrated alcohol，First by 0.3 ~ 0.7 part of IBA root-growing agent with 2 ~ 3 parts of dehydrated alcohol mixing，Mixed liquor A is obtained standby after stirring，Again 10 ~ 15 parts of Mel are added in suitable quantity of water，It is slowly heated to 35 ~ 40 DEG C，Then by 60 ~ 80 parts of amino-acid chelates、20 ~ 25 parts of compound vitamines、2 ~ 3 parts of gentiobioses、2 ~ 4 parts of brown alga oligoses、5 ~ 10 parts of nitrogen、6 ~ 8 parts of phosphorus、5 ~ 8 parts of potassium、3 ~ 5 parts of Caulis Miscanthis floriduli extracts add wherein，Mixed liquid B is obtained standby after stirring，Finally by mixed liquor A and mixed liquid B mix and blend，And the mass fraction regulating IBA root-growing agent that adds water is 25 ~ 30mg/kg；
(4) skewer cutting treatment and cuttage: first the cuttage bar prepared is put in K cryogenic treatment under 6 ~ 8 DEG C of conditions, and evaporate cuttage bar moisture, keeping its water content is 25 ~ 27%, take out after continuing 15 ~ 20min, then put the bottom of cuttage bar again to take out after taking root liquid after the heating soaks 1 ~ 2h and carry out cuttage immediately, by distance between rows and hills 6cm × 7cm cuttage in constructed cuttage ground, the cuttage degree of depth is 3 ~ 5cm；
(5) manage after cuttage: in the 1st ~ 10 day after cuttage, keeping relative air humidity is 90 ~ 95%, temperature is 22 ~ 26 DEG C, spray water every day 2 ~ 4 times, keep ground moistening, in 11st ~ 20 day, keeping relative air humidity is 85 ~ 90%, it is 13 ~ 16 DEG C that every afternoon controls temperature between 4:00 ~ 6.00, all the other time keeps temperature to be 20 ~ 25 DEG C, spray water every day 2 ~ 5 times, keep ground moistening, after 21st day, keeping relative air humidity is 80 ~ 90%, keeping temperature is 18 ~ 23 DEG C, spray water every day 3 ~ 5 times, keep ground moistening, occur can carrying out after a large amount of root systems transplanting operation until cuttage bar root.
Further, the preparation method of step (2) described disinfectant comprises the steps:
(1) jointly putting in the marmite filling yellow soil by 10 ~ 15 portions of Radix Glycyrrhizaes, 6 ~ 8 parts of reed Ba Zi and 3 ~ 5 part Rhizoma Menispermis, add hot terrine and carry out parch, stop parch when medical surfaces is buff, it is standby that taking-up elimination yellow soil obtains parch medicine；
(2) 8 ~ 10 parts of Herba Salviae Chinensiss, 5 ~ 10 parts of Flos Celosiae Cristataes, 12 ~ 17 parts of Melaleuca Alternifolia leaveves, 5 ~ 8 parts of citron leaves are put into jointly in the distilled water of its gross mass 3 ~ 4 times, boiling water filter after decocting 60 ~ 80min filtering residue and the first filtrate standby；
(3) being mixed by the filtering residue of the parch medicine of step (1) gained with step (2) gained in the dehydrated alcohol putting into its gross mass 6 ~ 8 times, the pressure controlled in dehydrated alcohol pot for solvent extraction is 5 ~ 8MPa, filter after continuing lixiviate 10 ~ 12h the second filtrate is standby；
(4), after the second filtrate of the first filtrate of step (2) gained and step (3) gained jointly being mixed, drug extract is obtained after heating the half being concentrated into its gross mass standby；
(5) putting in nitrogen environment by 20 ~ 25 parts of rice husks, heating is to 320 ~ 350 DEG C of carbonizations that carry out stir-frying, and being then comminuted into 100 orders, to obtain charring rice husk standby；
(6) by after 3 ~ 5 parts of useful mycopowder, 1 ~ 2 part of muscardine, 5 ~ 8 parts of chitins and 3 ~ 5 parts of attapulgite mix homogeneously, the solid particle that diameter is 1 ~ 2mm is made after allocating appropriate water into, it is evenly applied on the surface of solid particle after 4 ~ 7 parts of gelatin heating and melting again, namely makes inner core standby；
(7) being put into by the charring rice husk of step (5) gained in the drug extract of step (4) gained after moisture absorption 10 ~ 15min, then added wherein by 10 ~ 12 parts of starch, control rotating speed is 300 ~ 330rpm, obtains pasty state applicator standby after stirring 3 ~ 5min；
(8) the pasty state applicator of step (7) gained is coated uniformly on the core surface of step (6) gained, and after making the granule that diameter is 3.5 ~ 4.5mm, drying to water content is 10 ~ 13% namely obtain soil sterilants.
Further, the amino-acid chelate described in step (3) is made up of iron-amino acid chelate, zinc-amino acid chelate, magnesium amino acid chelate, chelating amino acids molybdenum.
Further, the preparation method of the Caulis Miscanthis floriduli extract described in step (3) is: take the root of Caulis Miscanthis floriduli plant, after cleaning the section shape being cut into 1.5 ~ 2.5cm, it is put in the dehydrated alcohol of its quality 5 ~ 10 times, filter to obtain lixiviating solution after lixiviate 15 ~ 20h, more namely obtain Caulis Miscanthis floriduli extract after lixiviating solution is concentrated into the 1/3 of original volume.
Further, step (5) described taking root liquid need to be heated to 28 ~ 30 DEG C.
There is advantages that
(1) the disinfectant major antibacterial composition prepared is Chinese herbal medicine extract, common soil pest and disease damage there is good killing effect, the cuttage bar initial stage is comparatively fragile, traditional disinfectant property of medicine is relatively big, and easily causes the harmful components residuals such as heavy metal in soil, affects the rooting development of cuttage bar, this disinfectant is then without this harm, and built-in inner core can increase again the content of soil probiotics, improve traditional disinfection and reduce the problem of probiotics in soil, promote the healthy development of cuttage bar further.
(2) the cuttage bar prepared is put under cryogenic, and reduce its internal moisture, can well reduce eye activity, prevent it from crossing premature eruption, then put it in the taking root liquid after heating, cause the situation that bottom temperature is higher, tip temperature is relatively low, improve the activity of bottom plant tissue, reduce top eye activity, prevent eye from crossing the problem of premature eruption and root growth competition for nutrients reduction survival rate further, additionally, this taking root liquid composition is equipped with rationally, the nutrition deposit of cuttage bar can be increased, can promote that again the separation of root cell is broken up；Cuttage bar places in taking root liquid after low temperature, processed, can improve its absorbtivity to nutrient substance, strengthens the action effect of taking root liquid.
(3) the inventive method integrated operation simple specification, under the combined effect of each operation so that the cuttage survival rate of Fructus Vitis viniferae is stable more than 98%, and the cutting seedling root amount of taking root educated is relatively big, educate to obtain time growing way shorter, overall uniformly vigorous, environment strong stress resistance.
Detailed description of the invention
Embodiment 1
A kind of method that Fructus Vitis viniferae rapid cuttage is taken root, comprises the steps:
(1) prepared by cuttage bar: selects Fructus Vitis viniferae semi-lignified annotinous branch, is cut into the cutting that length is 7 ~ 9cm, and lower end is cut into the shape of a hoof, upper end straight snips, and each cutting retains 5 ~ 7 eyes；
(2) cuttage ground preparation: by fluvial sand, Vermiculitum, turfy soil by weight mix homogeneously after 1:1:2 preparation, and carry out disinfection by disinfectant, then paved one-tenth length, width and height are the cuboid cuttage ground of 5m × 6m × 0.4m, and described disinfectant is made up of the material of following weight portion: 15 parts of Radix Glycyrrhizaes, 8 parts of Herba Salviae Chinensiss, 6 parts of Flos Celosiae Cristataes, 8 parts of reed Ba Zi, 15 parts of Melaleuca Alternifolia leaveves, 5 parts of citron leaves, 5 parts of Rhizoma Menispermis, 3 parts of useful mycopowder, 2 parts of muscardines, 5 parts of chitins, 3 parts of attapulgites, 4 parts of gelatin, 10 parts of starch, 23 parts of rice husks；
null(3) prepared by taking root liquid: weigh the material of following weight portion: 0.5 part of IBA root-growing agent、68 parts of amino-acid chelates、20 parts of compound vitamines、12 parts of Mel、3 parts of gentiobioses、2 parts of brown alga oligoses、8 parts of nitrogen、6 parts of phosphorus、5 parts of potassium、3 parts of Caulis Miscanthis floriduli extracts、3 parts of dehydrated alcohol，First by 0.5 part of IBA root-growing agent with 2 ~ 3 parts of dehydrated alcohol mixing，Mixed liquor A is obtained standby after stirring，Again 12 parts of Mel are added in suitable quantity of water，It is slowly heated to 35 ~ 40 DEG C，Then by 68 parts of amino-acid chelates、20 parts of compound vitamines、12 parts of Mel、3 parts of gentiobioses、2 parts of brown alga oligoses、8 parts of nitrogen、6 parts of phosphorus、5 parts of potassium、3 parts of Caulis Miscanthis floriduli extracts add wherein，Mixed liquid B is obtained standby after stirring，Finally by mixed liquor A and mixed liquid B mix and blend，And the mass fraction regulating IBA root-growing agent that adds water is 25 ~ 30mg/kg；
(4) skewer cutting treatment and cuttage: first the cuttage bar prepared is put in K cryogenic treatment under 6 ~ 8 DEG C of conditions, and evaporate cuttage bar moisture, keeping its water content is 25 ~ 27%, take out after continuing 15 ~ 20min, then put the bottom of cuttage bar again to take out after taking root liquid after the heating soaks 1 ~ 2h and carry out cuttage immediately, by distance between rows and hills 6cm × 7cm cuttage in constructed cuttage ground, the cuttage degree of depth is 3 ~ 5cm；
(5) manage after cuttage: in the 1st ~ 10 day after cuttage, keeping relative air humidity is 90 ~ 95%, temperature is 22 ~ 26 DEG C, spray water every day 2 ~ 4 times, keep ground moistening, in 11st ~ 20 day, keeping relative air humidity is 85 ~ 90%, it is 13 ~ 16 DEG C that every afternoon controls temperature between 4:00 ~ 6.00, all the other time keeps temperature to be 20 ~ 25 DEG C, spray water every day 2 ~ 5 times, keep ground moistening, after 21st day, keeping relative air humidity is 80 ~ 90%, keeping temperature is 18 ~ 23 DEG C, spray water every day 3 ~ 5 times, keep ground moistening, occur can carrying out after a large amount of root systems transplanting operation until cuttage bar root.
Further, the preparation method of step (2) described disinfectant comprises the steps:
(1) jointly putting in the marmite filling yellow soil by 15 portions of Radix Glycyrrhizaes, 8 parts of reed Ba Zi and 5 portions of Rhizoma Menispermis, add hot terrine and carry out parch, stop parch when medical surfaces is buff, it is standby that taking-up elimination yellow soil obtains parch medicine；
(2) 8 parts of Herba Salviae Chinensiss, 6 parts of Flos Celosiae Cristataes, 15 parts of Melaleuca Alternifolia leaveves, 5 parts of citron leaves are put into jointly in the distilled water of its gross mass 3 ~ 4 times, boiling water filter after decocting 60 ~ 80min filtering residue and the first filtrate standby；
(3) being mixed by the filtering residue of the parch medicine of step (1) gained with step (2) gained in the dehydrated alcohol putting into its gross mass 6 ~ 8 times, the pressure controlled in dehydrated alcohol pot for solvent extraction is 5 ~ 8MPa, filter after continuing lixiviate 10 ~ 12h the second filtrate is standby；
(4), after the second filtrate of the first filtrate of step (2) gained and step (3) gained jointly being mixed, drug extract is obtained after heating the half being concentrated into its gross mass standby；
(5) putting in nitrogen environment by 23 parts of rice husks, heating is to 320 ~ 350 DEG C of carbonizations that carry out stir-frying, and being then comminuted into 100 orders, to obtain charring rice husk standby；
(6) by after 3 parts of useful mycopowder, 2 parts of muscardines, 5 parts of chitins, 3 parts of attapulgite mix homogeneously, the solid particle that diameter is 1 ~ 2mm is made after allocating appropriate water into, it is evenly applied on the surface of solid particle after 4 parts of gelatin heating and melting again, namely makes inner core standby；
(7) being put into by the charring rice husk of step (5) gained in the drug extract of step (4) gained after moisture absorption 10 ~ 15min, then added wherein by 10 parts of starch, control rotating speed is 300 ~ 330rpm, obtains pasty state applicator standby after stirring 3 ~ 5min；
(8) the pasty state applicator of step (7) gained is coated uniformly on the core surface of step (6) gained, and after making the granule that diameter is 3.5 ~ 4.5mm, drying to water content is 10 ~ 13% namely obtain soil sterilants.
Further, the amino-acid chelate described in step (3) is made up of iron-amino acid chelate, zinc-amino acid chelate, magnesium amino acid chelate, chelating amino acids molybdenum.
Further, the preparation method of the Caulis Miscanthis floriduli extract described in step (3) is: take the root of Caulis Miscanthis floriduli plant, after cleaning the section shape being cut into 1.5 ~ 2.5cm, it is put in the dehydrated alcohol of its quality 5 ~ 10 times, filter to obtain lixiviating solution after lixiviate 15 ~ 20h, more namely obtain Caulis Miscanthis floriduli extract after lixiviating solution is concentrated into the 1/3 of original volume.
Further, step (5) described taking root liquid need to be heated to 28 ~ 30 DEG C.
Comparative example 1
This comparative example 1 is compared with embodiment 1, and except using quick lime as disinfectant in the preparation of (2) cuttage ground, all the other method steps are all identical.
Comparative example 2
This comparative example 2 is compared with embodiment 1, and except cuttage bar not being carried out low temperature and processed in step (4) skewer cutting treatment with cuttage, all the other method steps are all identical.
Comparative example 3
This comparative example 3 adopts the Fructus Vitis viniferae cuttage and seedling culture method of routine.
Choosing the Fructus Vitis viniferae of " huge Flos Rosae Rugosae " kind as experimental subject, cultivate by above-mentioned four kinds of methods respectively, following table is corresponding correction data:
| Embodiment 1 | Comparative example 1 | Comparative example 2 | Comparative example 3 | |
| Cuttage survival rate (%) | 98.6 | 92.4 | 90.8 | 80.2 |
Claims (5)
1. the method that a Fructus Vitis viniferae rapid cuttage is taken root, it is characterised in that comprise the steps:
(1) prepared by cuttage bar: selects Fructus Vitis viniferae semi-lignified annotinous branch, is cut into the cutting that length is 7 ~ 9cm, and lower end is cut into the shape of a hoof, upper end straight snips, and each cutting retains 5 ~ 7 eyes；
(2) cuttage ground preparation: by fluvial sand, Vermiculitum, turfy soil is by weight mix homogeneously after 1:1:2 preparation, and carry out disinfection by disinfectant, then paved one-tenth length, width and height are the cuboid cuttage ground of 5m × 6m × 0.4m, described disinfectant is made up of the material of following weight portion: 10 ~ 15 portions of Radix Glycyrrhizaes, 8 ~ 10 parts of Herba Salviae Chinensiss, 5 ~ 10 parts of Flos Celosiae Cristataes, 6 ~ 8 parts of reed Ba Zi, 12 ~ 17 parts of Melaleuca Alternifolia leaveves, 5 ~ 8 parts of citron leaves, 3 ~ 5 portions of Rhizoma Menispermis, 3 ~ 5 parts of useful mycopowder, 1 ~ 2 part of muscardine, 5 ~ 8 parts of chitins, 3 ~ 5 parts of attapulgites, 4 ~ 7 parts of gelatin, 10 ~ 12 parts of starch, 20 ~ 25 parts of rice husks；
null(3) prepared by taking root liquid: weigh the material of following weight portion: 0.3 ~ 0.7 part of IBA root-growing agent、60 ~ 80 parts of amino-acid chelates、20 ~ 25 parts of compound vitamines、10 ~ 15 parts of Mel、2 ~ 3 parts of gentiobioses、2 ~ 4 parts of brown alga oligoses、5 ~ 10 parts of nitrogen、6 ~ 8 parts of phosphorus、5 ~ 8 parts of potassium、3 ~ 5 parts of Caulis Miscanthis floriduli extracts、2 ~ 3 parts of dehydrated alcohol，First by 0.3 ~ 0.7 part of IBA root-growing agent with 2 ~ 3 parts of dehydrated alcohol mixing，Mixed liquor A is obtained standby after stirring，Again 10 ~ 15 parts of Mel are added in suitable quantity of water，It is slowly heated to 35 ~ 40 DEG C，Then by 60 ~ 80 parts of amino-acid chelates、20 ~ 25 parts of compound vitamines、2 ~ 3 parts of gentiobioses、2 ~ 4 parts of brown alga oligoses、5 ~ 10 parts of nitrogen、6 ~ 8 parts of phosphorus、5 ~ 8 parts of potassium、3 ~ 5 parts of Caulis Miscanthis floriduli extracts add wherein，Mixed liquid B is obtained standby after stirring，Finally by mixed liquor A and mixed liquid B mix and blend，And the mass fraction regulating IBA root-growing agent that adds water is 25 ~ 30mg/kg；
(4) skewer cutting treatment and cuttage: first the cuttage bar prepared is put in K cryogenic treatment under 6 ~ 8 DEG C of conditions, and evaporate cuttage bar moisture, keeping its water content is 25 ~ 27%, take out after continuing 15 ~ 20min, then put the bottom of cuttage bar again to take out after taking root liquid after the heating soaks 1 ~ 2h and carry out cuttage immediately, by distance between rows and hills 6cm × 7cm cuttage in constructed cuttage ground, the cuttage degree of depth is 3 ~ 5cm；
(5) manage after cuttage: in the 1st ~ 10 day after cuttage, keeping relative air humidity is 90 ~ 95%, temperature is 22 ~ 26 DEG C, spray water every day 2 ~ 4 times, keep ground moistening, in 11st ~ 20 day, keeping relative air humidity is 85 ~ 90%, it is 13 ~ 16 DEG C that every afternoon controls temperature between 4:00 ~ 6.00, all the other time keeps temperature to be 20 ~ 25 DEG C, spray water every day 2 ~ 5 times, keep ground moistening, after 21st day, keeping relative air humidity is 80 ~ 90%, keeping temperature is 18 ~ 23 DEG C, spray water every day 3 ~ 5 times, keep ground moistening, occur can carrying out after a large amount of root systems transplanting operation until cuttage bar root.
2. the method that a kind of Fructus Vitis viniferae rapid cuttage according to claim 1 is taken root, it is characterised in that the preparation method of step (2) described disinfectant comprises the steps:
(1) jointly putting in the marmite filling yellow soil by 10 ~ 15 portions of Radix Glycyrrhizaes, 6 ~ 8 parts of reed Ba Zi and 3 ~ 5 part Rhizoma Menispermis, add hot terrine and carry out parch, stop parch when medical surfaces is buff, it is standby that taking-up elimination yellow soil obtains parch medicine；
(2) 8 ~ 10 parts of Herba Salviae Chinensiss, 5 ~ 10 parts of Flos Celosiae Cristataes, 12 ~ 17 parts of Melaleuca Alternifolia leaveves, 5 ~ 8 parts of citron leaves are put into jointly in the distilled water of its gross mass 3 ~ 4 times, boiling water filter after decocting 60 ~ 80min filtering residue and the first filtrate standby；
(3) being mixed by the filtering residue of the parch medicine of step (1) gained with step (2) gained in the dehydrated alcohol putting into its gross mass 6 ~ 8 times, the pressure controlled in dehydrated alcohol pot for solvent extraction is 5 ~ 8MPa, filter after continuing lixiviate 10 ~ 12h the second filtrate is standby；
(4), after the second filtrate of the first filtrate of step (2) gained and step (3) gained jointly being mixed, drug extract is obtained after heating the half being concentrated into its gross mass standby；
(5) putting in nitrogen environment by 20 ~ 25 parts of rice husks, heating is to 320 ~ 350 DEG C of carbonizations that carry out stir-frying, and being then comminuted into 100 orders, to obtain charring rice husk standby；
(6) by after 3 ~ 5 parts of useful mycopowder, 1 ~ 2 part of muscardine, 5 ~ 8 parts of chitins and 3 ~ 5 parts of attapulgite mix homogeneously, the solid particle that diameter is 1 ~ 2mm is made after allocating appropriate water into, it is evenly applied on the surface of solid particle after 4 ~ 7 parts of gelatin heating and melting again, namely makes inner core standby；
(7) being put into by the charring rice husk of step (5) gained in the drug extract of step (4) gained after moisture absorption 10 ~ 15min, then added wherein by 10 ~ 12 parts of starch, control rotating speed is 300 ~ 330rpm, obtains pasty state applicator standby after stirring 3 ~ 5min；
(8) the pasty state applicator of step (7) gained is coated uniformly on the core surface of step (6) gained, and after making the granule that diameter is 3.5 ~ 4.5mm, drying to water content is 10 ~ 13% namely obtain soil sterilants.
3. the method that a kind of Fructus Vitis viniferae rapid cuttage according to claim 1 is taken root, it is characterised in that the amino-acid chelate described in step (3) is made up of iron-amino acid chelate, zinc-amino acid chelate, magnesium amino acid chelate, chelating amino acids molybdenum.
4. the method that a kind of Fructus Vitis viniferae rapid cuttage according to claim 1 is taken root, it is characterized in that, the preparation method of the Caulis Miscanthis floriduli extract described in step (3) is: take the root of Caulis Miscanthis floriduli plant, after cleaning the section shape being cut into 1.5 ~ 2.5cm, it is put in the dehydrated alcohol of its quality 5 ~ 10 times, filter to obtain lixiviating solution after lixiviate 15 ~ 20h, more namely obtain Caulis Miscanthis floriduli extract after lixiviating solution is concentrated into the 1/3 of original volume.
5. the method that a kind of Fructus Vitis viniferae rapid cuttage according to claim 1 is taken root, it is characterised in that step (5) described taking root liquid need to be heated to 28 ~ 30 DEG C.
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| CN106472061A (en) * | 2016-09-20 | 2017-03-08 | 甘肃省农业科学院经济作物与啤酒原料研究所（甘肃省农业科学院中药材研究所） | A kind of Radix Glycyrrhizae cuttage breeding method |
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