CN113508747A - Method for inducing robust growth of mango seedling root system - Google Patents
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Soil Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention relates to a method for inducing the robust growth of mango seedling root systems. The method comprises the following steps: 1) selecting mango fruits which reach maturity; 2) removing mango pulp, cutting mango kernels, and taking out seeds; 3) sterilizing the seeds with 0.2% carbendazim for 15-18min, and washing; 4) soaking mango seeds in a solution of 6-BA1-4mg/L + NAA0.2-0.8mg/L for 14-15 h; 5) the seeds can fully absorb the two hormones and water in the solution; 6) selecting a plastic seedling raising bag, and filling a culture medium; 7) and (4) planting the fully soaked mango seeds into a seedling bag, and then covering river sand until the mango seeds germinate and root systems grow. From the beginning of mango seed germination, the root length, the thickness and the fibrous root number are rapidly increased, and a good effect is obtained.
Description
Technical Field
The invention relates to the technical field of plant cultivation, in particular to a method for inducing the robust growth of mango germchit root systems.
Background
Naphthylacetic acid (NAA), a broad-spectrum plant growth regulator, promotes rapid rooting and germination of the roots, stems of plants. 6-benzylaminopurine (6-BA), an artificially synthesized cytokinin. The action of the compound is the same as that of natural cytokinin, and the compound can promote cell division and the elongation growth of roots, stems and leaves, but the action time is long and the effect is more stable. The optimum dosage and proportion of the two hormones are screened, and the growth of plant roots can be induced.
1. Application studies of others in seed treatment
1) Seeds of the common tobacco variety W38 were treated with different levels of 6-BA, NAA, and the results showed that: when MS +6-BA 0.25mg/L + NAA0.1mg/L, the budding rate is 100%. When MS +6-BA 1.0mg/L + NAA0.1mg/L, the rooting rate is 100%.
2) The seeds of Arabidopsis are respectively soaked by GA3, NAA and 6-BA with different levels, and the result shows that: the GA3 is optimally treated when the GA3 is 2 mg/L7 h and 4 mg/L7 h; the effect is better when the NAA is 0.4 mg/L; the best effect is achieved when the 6-BA is 0.4mg/L for 18 h.
3) The seed soaking treatment of the white seed and the seed by using different levels of 6-BA, NAA and SA shows that: the overall promoting effect on the seed seedling rate is 6-BA > NAA > SA, the effect of 1.0 mg/L6-BA treatment on the three hormones is the best, and the effect of 0.5mg/L NAA treatment is the second.
2. Application research of others in tissue culture
1) The test-tube plantlet of the phoenix-headed ginger is processed by using 6-BA and NAA with different levels, and the result shows that: when the 6-BA accounts for 3.0mg/L and the NAA accounts for 0.5 mg/LNAA, the rooting method is suitable for the ginger to root. When the ratio of 6-BA 5.0 mg/L and NAA0.1mg/L is favorable for tillering of ginger, and when the ratio of 6-BA0.2mg/L and NAA0.5mg/L is favorable for the growth of rhizome of ginger.
2) The callus of the cotyledon stage of the cabbage with three different ripeness, namely, the Chinese cabbage of eleven (early maturity 65 d), the Jingfeng (middle maturity 90 d) and the late harvest (late maturity 110 d) is treated by using NAA and 6-BA with different levels, and the results show that: the induction rate of the calli can reach 96.7 percent at the highest when the hypocotyl of Jingfeng is 6-BA3.0 mg/L + NAA0.45 mg/L, the callus cluster buds which grow fast are induced from faint yellow to light yellow green crisp soft clusters when the hypocotyl of Taifeng is 6-BA 6.0 mg/L + NAA0.6mg/L, and the callus cluster buds which grow fast are induced from the hypocotyl of Zhongganelen at 6-BA3.0 mg/L + NAA0.45 mg/L, the middle part of the hypocotyl of Zhongganelen is green, and the periphery of the hypocotyl is semitransparent crisp soft clusters and the cluster buds can reach 95 percent.
3) The wild eggplant callus is processed by using NAA and 6-BA with different levels, and the result shows that: the hormone proportion of 6-BA3mg/L and NAA0.2mg/L is the best for inducing the callus of the wild eggplant to bud.
4) The hypocotyl of bougainvillea spectabilis is induced by using 6-BA and NAA at different levels, and the result shows that: the culture medium is MS + NAA0.05mg/L +6-BA 3.0mg/L with the best adventitious bud proliferation, and the culture medium is MS + NAA0.1mg/L +6-BA 1.0mg/L with the best strong seedling.
5) The 6-BA and NAA with different levels are used for inducing the liana canna kind Sunparasol, and the result shows that: when the starting culture medium is MS +6-BA 4mg/L + NAA0.1mg/L, the germination rate is as high as 93.33%; in the subculture stage, the proliferation amount is the most and the growth is robust in the MS +6-BA2mg/L + NAA0.1mg/L culture medium.
6) The dendrobium officinale is induced by using 6-BA and NAA with different levels, and the result shows that: the most suitable culture medium for root induction is MS +6-BA0.75 mg/L + NAA0.5 mg/L.
7) The anoectochilus formosanus is induced by using 6-BA and NAA at different levels, and the result shows that: the culture medium is improved MS +6-BA3mg/L + NAA0.5mg/L, the bud germination is fast and strong, the multiplication multiple is high, the growth speed is fast, the seedlings are neat, and the requirement of fast seedling culture can be met.
8) The wild pinellia ternata is induced by using 6-BA and NAA at different levels, and the result shows that: the best induction culture medium is MS +6-BA2.0mg/L + NAA0.3mg/L; the optimal proliferation culture medium is MS +6-BA2.0mg/L + NAA0.2mg/L.
9) The three varieties of alum root 'waterfall', 'maltose' and 'purple palace' are induced by using 6-BA and NAA with different levels, and research shows that: the optimal induction and differentiation culture medium of 'waterfall', 'maltose' and 'purple palace' is MS +6-BA0.5mg/L + NAA0.05mg/L; the optimal subculture medium of 'waterfall' and 'maltose' is MS +6-BA0.1mg/L + NAA0.01mg/L, and the optimal subculture medium of 'purple palace' is MS +6-BA0.2mg/L + NAA0.02mg/L.
10) Different levels of 6-BA and NAA are used for inducing Lanzhou lily, and the research shows that: the lily scales are treated by the combination of 6-BA1-2mg/L and NAA0.4-0.8mg/L, and the promotion effect on the differentiation buds and rooting of the lily scales is stronger than that of other treatments.
11) The euonymus petiolus is induced by using 6-BA and NAA at different levels, and research shows that: the induction rate is 86.2% when the concentration ratio of the culture medium phytohormone is MS +6-BA2mg/L + NAA0.1mg/L, and the tissue culture differentiation effect of the euonymus petiolatus is better.
12) The garlic stem tip is induced by using 6-BA and NAA with different levels, and the research shows that: the optimal differentiation medium is MS +6-BA1mg/L + NAA0.1mg/L, and the optimal subculture medium is 1/2MS +6-BA0.2
mg/L+NAA0.2mg/L。
13) The sweet potato stem tip meristem is induced by using 6-BA and NAA with different levels, and the result shows that: when the culture medium is 1/2MS +6-BA0.2mg/L + NAA0.2mg/L, the seedling rate of the culture medium is high.
3. Application study of others in other root strengthening ways
1) The radix pseudostellariae root strengthening high-yield technology utilizes the characteristics of plants to perform topping on the radix pseudostellariae, so that the radix pseudostellariae is root strengthening and high in yield.
2) Utilizes the plant growth retardant root strengthening powder to promote the growth of the root system of the codonopsis pilosula.
Reinecking spring]The overwintering cucumber root strengthening method increases soil air permeability and reduces soil hardening through re-applying organic fertilizer, and promotes cucumber rooting through improving soil.
3) The biological fertilizer-Zhao's Zhuang Gen element is used to promote the root growth of economic root crops such as potato, etc. so as to achieve the purpose of increasing the yield.
4) The technology for raising and strengthening the roots of the fruit trees keeps stable moisture through grass covering, sprinkling irrigation or drip irrigation, thereby strengthening the protection of surface roots and promoting the high quality and high yield of the fruit trees.
At present, no report of culturing mango strong root seedlings by NAA and 6-BA at different levels is found, and the application of the technology in actual production is not found.
Disclosure of Invention
The invention aims to provide a method for inducing the robust growth of mango seedling root systems. The method realizes the growth promotion of mango root systems and achieves the purpose of mango seedling and root strengthening by screening the hormone dosage and the proportion, soaking the seeds in two hormone solutions with different proportions and cultivating the seeds.
The method for inducing the mango seedling root system to grow robustly comprises the following steps:
1) selecting mango fruits which reach maturity;
2) removing mango pulp, cutting mango kernels, and taking out seeds;
3) sterilizing the seeds with 0.2% carbendazim for 15-18min, and washing;
4) soaking mango seeds in a solution of 6-BA1-4mg/L + NAA0.2-0.8mg/L for 14-15h to enable the seeds to fully absorb two hormones and water in the solution;
5) cleaning river sand with clear water, screening gravel with a sand stone, and naturally drying;
6) filling river sand into a plastic seedling raising bag, wherein the sand filling amount is 2/3 of the volume of the seedling raising bag, and the depth is 8-10 cm;
7) and (4) planting the fully soaked mango seeds into a seedling bag, and then covering river sand until the mango seeds germinate and root systems grow.
Furthermore, the specification of the sand sieve is 1.18 mm.
Furthermore, the specification of the plastic seedling raising bag is 12cm x 10 cm.
The method of the invention can achieve the following positive effects:
the method can obviously improve the growth amount of the root system of the mango seedlings, and the length, thickness and fibrous root number of the root system are rapidly increased from the beginning of mango seed germination, so that a better effect is obtained. NAA has the effect of promoting growth and simultaneously has the effect of inducing plant rooting, 6-BA has the effect of promoting cell division, and 6-BA and NAA are mixed for use to play a synergistic effect and better promote the growth of mango seedling roots.
Detailed Description
It will be appreciated by those skilled in the art that the following examples are illustrative of the invention only and should not be taken as limiting the scope of the invention. The examples do not specify particular techniques or conditions, and are performed according to the techniques or conditions described in the literature in the art or according to the product specifications. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available by purchase.
Example 1:
1) selecting mango fruits which reach maturity;
2) removing mango pulp; cutting off mango kernels, and taking out seeds;
3
3) sterilizing the seeds with 0.2% carbendazim for 15min, and washing;
4) soaking mango seeds in a solution of 6-BA1mg/L and NAA0.2mg/L for 14h to ensure that the seeds fully absorb two hormones and water;
5) selecting a plastic seedling raising bag with the specification of 12cm x 10cm, and filling river sand, wherein the sand filling amount is 2/3 (the depth is 8 cm) of the volume of the seedling raising bag;
6) and (4) planting the fully soaked mango seeds into a seedling raising bag, and then covering river sand.
Observing from the beginning of seed germination, when the root system grows for 20d, 27d, 34d, 41d, 48d, the root system length is respectively: 65.33mm, 75.65mm, 85.32mm, 115.48mm and 143.73mm, and the root thickness is respectively as follows: 1.12mm, 1.42mm, 1.43mm and 2.40mm, and the number of the fiber roots is respectively as follows: 12 strips, 17 strips, 25 strips, 28 strips and 43 strips.
The growth of the root system is relatively slow from the beginning of mango seed germination, and the hormone has no obvious effect on promoting the growth of the root system.
Example 2:
1) selecting mango fruits which reach maturity;
2) removing mango pulp, cutting mango kernels, and taking out seeds;
3) sterilizing the seeds with 0.2% carbendazim for 15min, and washing;
4) mango seeds are soaked in a solution of 6-BA2mg/L and NAA0.4mg/L for 14.5h, so that the seeds can fully absorb two hormones and water;
5) selecting a plastic seedling raising bag with the specification of 12cm x 10cm, and filling river sand, wherein the sand filling amount is 2/3 (the depth is 9 cm) of the volume of the seedling raising bag;
6) and (4) planting the fully soaked mango seeds into a seedling raising bag, and then covering river sand.
Observing from the beginning of seed germination, when the root system grows for 20d, 27d, 34d, 41d, 48d, the root system length is respectively: 118.65mm, 130.37mm, 135.94mm, 164.33mm and 235.02mm, and the root thickness is respectively as follows: 1.30mm, 1.32mm, 1.39mm, 2.30mm and 3.37mm, and the number of the fibrous roots is respectively as follows: 17, 27, 44, 61 and 70.
From the beginning of mango seed germination, the root system grows faster, and hormone has obvious promotion effect on the root system, so that a better effect is obtained.
Example 3:
1) selecting mango fruits which reach maturity;
2) removing mango pulp, cutting mango kernels, and taking out seeds;
3) sterilizing the seeds with 0.2% carbendazim for 15min, and washing;
4) mango seeds are soaked in a solution of 6-BA3mg/L + NAA0.8mg/L for 14h, so that the seeds can fully absorb two hormones and water;
5) selecting a plastic seedling raising bag with the specification of 12cm x 10cm, and filling river sand, wherein the sand filling amount is 2/3 (the depth is 8 cm) of the volume of the seedling raising bag;
6) and (4) planting the fully soaked mango seeds into a seedling raising bag, and then covering river sand.
Observing from the beginning of seed germination, when the root system grows for 20d, 27d, 34d, 41d, 48d, the root system length is respectively: 96.9mm, 143.01mm, 157.14mm, 189.14mm and 257.03mm, and the root thickness is respectively as follows: 1.31mm, 1.44mm, 1.91mm, 3.14mm and 3.81mm, and the number of the fibrous roots is respectively as follows: 17, 34, 65, 72 and 76.
From the beginning of mango seed germination, the root system grows faster, and hormone has obvious promotion effect on the root system, so that a better effect is obtained.
Example 4:
1) selecting mango fruits which reach maturity;
2) removing mango pulp, cutting mango kernels, and taking out seeds;
3) sterilizing the seeds with 0.2% carbendazim for 15min, and washing;
4) soaking mango seeds in a solution of 6-BA4mg/L + NAA0.6mg/L for 15h to ensure that the seeds fully absorb two hormones and water;
5) selecting a plastic seedling raising bag with the specification of 12cm x 10cm, and filling river sand, wherein the sand filling amount is 2/3 (the depth is 10 cm) of the volume of the seedling raising bag;
6) and (4) planting the fully soaked mango seeds into a seedling raising bag, and then covering river sand.
Observing from the beginning of seed germination, when the root system grows for 20d, 27d, 34d, 41d, 48d, the root system length is respectively: 101.5mm, 131.24mm, 165.94mm, 180.39mm and 237.43mm, the root thickness is respectively as follows: 1.38mm, 2.30mm, 2.43mm, 3.14mm and 3.35mm, and the number of the fiber roots is respectively as follows: 29, 45, 46 and 59.
From the beginning of mango seed germination, the root system grows faster, and hormone has obvious promotion effect on the root system, so that a better effect is obtained.
Example 5:
1) selecting mango fruits which reach maturity;
2) removing mango pulp, cutting mango kernels, and taking out seeds;
3) sterilizing the seeds with 0.2% carbendazim for 15min, and washing;
4) mango seeds are soaked in a solution of 6-BA4mg/L + NAA0.8mg/L for 14h, so that the seeds can fully absorb two hormones and water;
5) selecting a plastic seedling raising bag with the specification of 12cm x 10cm, and filling river sand, wherein the sand filling amount is 2/3 (the depth is 8.5 cm) of the volume of the seedling raising bag;
6) and (4) planting the fully soaked mango seeds into a seedling raising bag, and then covering river sand.
Observing from the beginning of seed germination, when the root system grows for 20d, 27d, 34d, 41d, 48d, the root system length is respectively: 61.27mm, 104.98mm, 126.11mm, 140.05mm and 171.2mm, and the root thickness is respectively as follows: 0.74mm, 1.56mm, 2.11mm, 3.15mm and 3.56mm, and the number of the fiber roots is respectively as follows: 17, 22, 32, 35 and 41.
In the above examples, different hormones were applied to obtain different results, and the root growth rates of examples 2-4 were all increased rapidly, with example 3 increasing most.
The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (3)
1. A method for inducing the robust growth of mango seedling root lines is characterized by comprising the following steps: the method comprises the following steps:
selecting mango fruits which reach maturity;
removing mango pulp, cutting mango kernels, and taking out seeds;
sterilizing the seeds with 0.2% carbendazim for 15-18min, and washing;
soaking mango seeds in a solution of 6-BA1-4mg/L + NAA0.2-0.8mg/L for 14-15h to enable the seeds to fully absorb two hormones and water in the solution;
cleaning river sand with clear water, screening gravel with a sand stone, and naturally drying;
filling river sand into a plastic seedling raising bag, wherein the sand filling amount is 2/3 of the volume of the seedling raising bag, and the depth is 8-10 cm;
and (4) planting the fully soaked mango seeds into a seedling bag, and then covering river sand until the mango seeds germinate and root systems grow.
2. The method for inducing the robust growth of mango seedling roots according to claim 1, characterized in that: the specification of the sand and stone sieve is 1.18 mm.
3. The method for inducing the robust growth of mango seedling roots according to claim 1, characterized in that: the specification of the plastic seedling raising bag is 12cm x 10 cm.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110813158.0A CN113508747A (en) | 2021-07-19 | 2021-07-19 | Method for inducing robust growth of mango seedling root system |
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| CN117598199A (en) * | 2024-01-24 | 2024-02-27 | 云南农业大学 | Alum root crossbreeding method |
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