CN114747378A - Grafting method for same-genus fruit tree variety - Google Patents

Grafting method for same-genus fruit tree variety Download PDF

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Publication number
CN114747378A
CN114747378A CN202210538811.1A CN202210538811A CN114747378A CN 114747378 A CN114747378 A CN 114747378A CN 202210538811 A CN202210538811 A CN 202210538811A CN 114747378 A CN114747378 A CN 114747378A
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grafting
stock
scion
fruit tree
cut
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Inventor
代作光
钟杰
青婷
陈洋
王亮
陈兵
王茂成
陈燕霞
刘丽
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Sichuan Hongguang Agricultural Development Co ltd
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Sichuan Hongguang Agricultural Development Co ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • A01G2/30Grafting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005Cultivation methods
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/06Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants

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  • Life Sciences & Earth Sciences (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Ecology (AREA)
  • Forests & Forestry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cultivation Of Plants (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The invention discloses a grafting method of congeneric fruit tree varieties, which comprises the following steps: selecting a plant which grows robustly as a stock, and cutting the stock at a height needing grafting; selecting semi-lignified branches or twigs without obvious insect damage of fruit trees as scions, removing terminal buds and leaves of the selected branches, and only keeping leafstalks; beveling a cut in the middle or slightly deviated position of the rootstock; cutting the bottom of the scion, and soaking the cut scion in auxin; inserting the processed scion into the incision of the stock with the bud upward to ensure that the cut surface of the scion is tightly attached to the cut surface of the stock, and tightly winding and sealing the whole grafting part by using a film to ensure that the grafting part does not leak; and after the branches germinate, cutting the film at the germinating positions. The grafting method of the invention can produce various fruits of the same genus on the same fruit tree, thus fundamentally solving the problem of single variety of fruits on one fruit tree.

Description

Grafting method for same-genus fruit tree variety
Technical Field
The invention relates to the technical field of plant cultivation, in particular to a grafting method of a congeneric fruit tree variety.
Background
The taxonomic classification of plants is: phylum, subgenus, class, order, family, subfamily, line, family, subfamily, genus and species. The plants can be divided into angiosperms, gymnosperms, bryophytes, ferns and algae plants, wherein the angiosperms can bloom and breed fruits, the gymnosperms originate from ancient times, the ferns comprise Stenoloma chusanum, Stenoloma chusanum and the like, the algae plants have simple structures, the number of the bryophytes is large, and the variety of the bryophytes is more than ten thousand and eighty thousand, so the life is tenacious.
Grafting is one of the artificial propagation methods of plants, that is, the branch or bud of one plant is grafted to the stem or root of the other plant, so that the two parts which are connected together grow into a complete plant. The grafting method is divided into a branch grafting method and a bud grafting method. The grafting is carried out by utilizing the function of wound healing of the plant after being wounded. The grafting is to take the scion from good and good mother, graft the scion to the stock chosen with different grafting methods, can breed the seedling fast, keep the good property of the mother, and improve the resistance of the scion, it is one of the main ways to solve the nursery stock production in the nursery stock trade at present.
During grafting, the scion is tightly combined with the cambium of the stock, and the grafted branch or bud is called as a scion; the grafted plant is called stock or table wood. The grafted seedling becomes the upper part or top of the plant body, and the grafted stock becomes the root part of the plant body.
In the production process, grafting has a positive effect on the improvement of crop varieties and the propagation of fruit trees. In the prior art, potted plants or fruit trees purchased by people are often single varieties, or only one variety is usually grafted on one tree during grafting, so that people can usually bloom one flower on one tree, and then one fruit or at most two fruits are grafted on one tree, the variety is single, the flowering phase of a single plant is short, and different flowers can not be bloomed in different flowering phases, and further the economic value is reduced.
Disclosure of Invention
The invention aims to provide a grafting method for the same-genus fruit tree variety, which is used for solving the technical problems that the same grafted fruit tree can not produce various fruits and the variety is single in the prior art.
In order to achieve the above object, an embodiment of the present invention provides a method for grafting a fruit tree variety of the same genus, comprising the following steps:
step (1): selecting a plant which grows robustly as a stock, and cutting off the stock at a height position which needs grafting;
step (2): selecting semi-lignified branches or twigs of fruit trees without obvious insect damage as scions, removing terminal buds and leaves of the selected branches, and only keeping leaf stalks;
and (3): a cut is obliquely cut in the middle of the rootstock or a slightly deviated position;
And (4): cutting the bottom of the scion, and soaking the cut scion in auxin;
and (5): inserting the processed scion into the incision of the stock with the bud upward to ensure that the cut surface of the scion is tightly attached to the cut surface of the stock, and tightly winding and sealing the whole grafting part by using a film to ensure that the grafting part does not leak;
and (6): after the branches germinate, cutting the film at the germinating positions;
and (7): grafting various fruit trees of the same genus on the same stock by adopting the grafting method from the step (1) to the step (6);
and (8): after grafting, the stock is watered, illuminated and fertilized, and the film is removed after the joint is healed for 1-2 months.
In one preferable scheme of the invention, in the step (1), the rootstock is a plant which is 3-4 years old and has normal growth and no plant diseases and insect pests.
In one preferable scheme of the invention, in the step (1), the cross section of the rootstock is flat and does not damage cortex.
In one preferable scheme of the invention, in the step (2), the branches are annual mature branches, and the length of the branches is 3cm-7 cm.
In one preferable scheme of the invention, in the step (3), the depth of the cut is 2cm-3cm, and the cut is ensured to be flat and smooth.
In one preferable embodiment of the present invention, in the step (4), the cutting plane at the bottom of the scion and the round section of the branch form an angle of 45 °, and the auxin is at least one of naphthylacetic acid and indoleacetic acid.
In one preferable scheme of the invention, in the step (4), the cutting plane at the bottom of the scion and the round section of the branch form an angle of 45 degrees, and the auxin is a mixture of naphthylacetic acid and indoleacetic acid.
In one preferable scheme of the invention, in the step (4), the soaking time in auxin before scion grafting is 3-8 h, the concentration of naphthylacetic acid is 10-30 mg/L, the concentration of indoleacetic acid is 5-8 mg/L, and the mass ratio of naphthylacetic acid to indoleacetic acid is 1: 1-3.
In one preferable embodiment of the present invention, in the step (5), the whole graft site is tightly wound with 2 layers of films, and the films are sterilized before use, wherein the films are plastic films, the thickness of the plastic films is 0.003cm-0.004cm, and the width of the plastic films is 2cm-3 cm.
In conclusion, the beneficial effects of the invention are as follows:
1. the fruit tree obtained by grafting can bear fruits of different varieties on the same tree, so that the problem of single fruit variety on one fruit tree is fundamentally solved, and the economic value and the productivity of the fruits are greatly improved.
2. The grafting method can be applied to all plants so as to improve the value and market capacity of crops; meanwhile, the variety of the plant can be increased, the plant combination can be customized according to market demands, and the economic value of the plant is greatly improved. The grafting method provided by the invention has important significance in scientific research, agricultural production and market economy.
Detailed Description
Example 1
A grafting method of congeneric fruit tree varieties comprises the following steps:
step (1): selecting 3-4-year-old plants which are normal in growth, strong in growth vigor and free of diseases and insect pests as stocks, fertilizing the stocks one week before grafting, cutting off the stocks at the height needing grafting after the stocks are selected, and enabling the sections of the stocks to be smooth and not to damage cortex;
step (2): selecting annual mature semi-lignified branches without obvious insect pests of fruit trees in the same genus as the rootstocks as scions, removing terminal buds and leaves of the selected branches, only preserving leaf stalks, pruning the scions into 3cm-7cm and preserving 2-4 buds, wherein the number of the scions is determined according to the number of varieties to be grafted;
the grafting time is as follows: grafting before spring germination from late February to middle March, and controlling the temperature at 15-20 ℃;
and (3): during grafting, a cut is obliquely cut in the middle or slightly deviated position of the stock, the cut is guaranteed to be flat and smooth, and the depth of the cut is 2cm-3 cm;
and (4): cutting the bottoms of any two outer sides of the scions, wherein the cutting surfaces at the bottoms of the scions form an angle of 45 degrees with the round section of a branch, the cutting surfaces are 1cm-2cm long, the cut scions are soaked in auxin for 5 hours, the auxin is a mixture of naphthylacetic acid and indoleacetic acid, and the mass ratio of the naphthylacetic acid to the indoleacetic acid is 1: 2, wherein the concentration of the naphthylacetic acid is 20mg/L, and the concentration of the indoleacetic acid is 7 mg/L;
And (5): inserting the processed scion into the incision of the stock, aligning to a cambium, enabling the eye to be upward, ensuring that the cut surface of the scion is tightly attached to the cut surface of the stock, winding and sealing the whole grafting part by using a layer of film, and then winding a layer of film on the grafting part to ensure that the grafting part does not leak, wherein the film is a plastic film, the thickness of the plastic film is 0.003cm-0.004cm, and the width of the plastic film is 2cm-3 cm;
and (6): after the branches germinate, the thin film at the germination position is cut off, so that the germination can grow out smoothly;
and (7): grafting various fruit trees of the same genus on the same stock by adopting the grafting method from the step (1) to the step (6);
and (8): after grafting, watering, illuminating and fertilizing the stock, keeping sufficient water and fertilizer supply of the plants, timely pinching and trimming to avoid consuming excessive nutrients, removing the film after the joint is healed after grafting for 1-2 months, and simultaneously counting the survival rate of grafting after 60 days of grafting.
Example 2
A grafting method of congeneric fruit tree varieties comprises the following steps:
step (1): selecting 3-4-year-old plants which are normal in growth, strong in growth vigor and free of diseases and insect pests as stocks, fertilizing the stocks one week before grafting, cutting off the stocks at the height needing grafting after the stocks are selected, and enabling the sections of the stocks to be smooth and not to damage cortex;
Step (2): selecting annual mature semi-lignified branches without obvious insect pests of fruit trees in the same genus as the rootstocks as scions, removing terminal buds and leaves of the selected branches, only keeping petioles, trimming the scions into 3cm-7cm and 2-4 remained buds, wherein the number of the scions is determined according to the number of varieties to be grafted;
the grafting time is as follows: grafting is carried out before spring germination from late February to middle March, and the temperature during grafting is controlled to be 15-20 ℃;
and (3): during grafting, a cut is obliquely cut in the middle or a slightly deviated position of the stock, the cut is ensured to be flat and smooth, and the depth of the cut is 2cm-3 cm;
and (4): cutting the bottoms of any two outer sides of the scions, wherein the cutting surfaces at the bottoms of the scions form an angle of 45 degrees with the round section of a branch, the cutting surfaces are 1cm-2cm long, the cut scions are soaked in auxin for 3 hours, the auxin is a mixture of naphthylacetic acid and indoleacetic acid, and the mass ratio of the naphthylacetic acid to the indoleacetic acid is 1: 1, wherein the concentration of the naphthylacetic acid is 10mg/L, and the concentration of the indoleacetic acid is 8 mg/L;
and (5): inserting the processed scion into the incision of the stock, aligning to the cambium, enabling the eye to be upward, ensuring that the cut surface of the scion is tightly attached to the cut surface of the stock, winding and sealing the whole grafting part by using 1 layer of film, and then winding 1 layer of film on the grafting part to ensure that the grafting part does not leak, wherein the film is a plastic film, the thickness of the plastic film is 0.003cm-0.004cm, and the width of the plastic film is 2cm-3 cm;
And (6): after the branches germinate, the thin film at the germination position is cut off, so that the germination can grow out smoothly;
and (7): grafting various fruit trees of the same genus on the same stock by adopting the grafting method from the step (1) to the step (6);
and (8): after grafting, watering, illuminating and fertilizing the stock, keeping sufficient water and fertilizer supply of the plants, timely pinching and trimming to avoid consuming excessive nutrients, removing the film after the joint is healed after grafting for 1-2 months, and simultaneously counting the survival rate of grafting after 60 days of grafting.
Example 3
A grafting method of congeneric fruit tree varieties comprises the following steps:
step (1): selecting 3-4-year-old plants which are normal in growth, strong in growth vigor and free of diseases and insect pests as stocks, fertilizing the stocks one week before grafting, cutting off the stocks at the height needing grafting after the stocks are selected, and enabling the sections of the stocks to be smooth and not to damage cortex;
step (2): selecting annual mature semi-lignified branches without obvious insect pests of fruit trees in the same genus as the rootstocks as scions, removing terminal buds and leaves of the selected branches, only preserving leaf stalks, pruning the scions into 3cm-7cm and preserving 2-4 buds, wherein the number of the scions is determined according to the number of varieties to be grafted;
The grafting time is as follows: grafting before spring germination from late February to middle March, and controlling the temperature at 15-20 ℃;
and (3): during grafting, a cut is obliquely cut in the middle or slightly deviated position of the stock, the cut is guaranteed to be flat and smooth, and the depth of the cut is 2cm-3 cm;
and (4): cutting the bottoms of any two outer sides of the scions, wherein the cutting surfaces at the bottoms of the scions form an angle of 45 degrees with the round section of a branch, the cutting surfaces are 1cm-2cm long, the cut scions are soaked in auxin for 8 hours, the auxin is a mixture of naphthylacetic acid and indoleacetic acid, and the mass ratio of the naphthylacetic acid to the indoleacetic acid is 1: 3, wherein the concentration of the naphthylacetic acid is 30mg/L, and the concentration of the indoleacetic acid is 5 mg/L;
and (5): inserting the processed scion into the incision of the stock, aligning to the cambium, enabling the eye to be upward, ensuring that the cut surface of the scion is tightly attached to the cut surface of the stock, winding and sealing the whole grafting part by using 1 layer of film, and then winding 1 layer of film on the grafting part to ensure that the grafting part does not leak, wherein the film is a plastic film, the thickness of the plastic film is 0.003cm-0.004cm, and the width of the plastic film is 2cm-3 cm;
and (6): after the branches germinate, the thin film at the germinating positions is cut off, so that the germination can grow out smoothly;
And (7): grafting various fruit trees of the same genus on the same stock by adopting the grafting method from the step (1) to the step (6);
and (8): after grafting, watering, illuminating and fertilizing the stock, keeping sufficient water and fertilizer supply of the plants, timely pinching and trimming to avoid consuming excessive nutrients, removing the film after the joint is healed after grafting for 1-2 months, and simultaneously counting the survival rate of grafting after 60 days of grafting.
Example 4
A grafting method of congeneric fruit tree varieties comprises the following steps:
step (1): selecting 3-4-year-old plants which are normal in growth, strong in growth vigor and free of diseases and insect pests as stocks, fertilizing the stocks one week before grafting, cutting off the stocks at the height needing grafting after the stocks are selected, and enabling the sections of the stocks to be smooth and not to damage cortex;
step (2): selecting annual mature semi-lignified branches without obvious insect pests of fruit trees in the same genus as the rootstocks as scions, removing terminal buds and leaves of the selected branches, only preserving leaf stalks, pruning the scions into 3cm-7cm and preserving 2-4 buds, wherein the number of the scions is determined according to the number of varieties to be grafted;
the grafting time is as follows: grafting before spring germination from late February to middle March, and controlling the temperature at 15-20 ℃;
And (3): during grafting, a cut is obliquely cut in the middle or slightly deviated position of the stock, the cut is guaranteed to be flat and smooth, and the depth of the cut is 2cm-3 cm;
and (4): cutting the bottoms of any two outer sides of the scions, wherein the cutting surfaces at the bottoms of the scions form an angle of 45 degrees with the round section of a branch, the cutting surfaces are 1cm-2cm long, the cut scions are soaked in auxin for 6 hours, the auxin is a mixture of naphthylacetic acid and indoleacetic acid, and the mass ratio of the naphthylacetic acid to the indoleacetic acid is 2: 3, wherein the concentration of the naphthylacetic acid is 15mg/L, and the concentration of the indoleacetic acid is 6 mg/L;
and (5): inserting the processed scion into the incision of the stock, aligning to the cambium, enabling the eye to be upward, ensuring that the cut surface of the scion is tightly attached to the cut surface of the stock, winding and sealing the whole grafting part by using 1 layer of film, and then winding 1 layer of film on the grafting part to ensure that the grafting part does not leak, wherein the film is a plastic film, the thickness of the plastic film is 0.003cm-0.004cm, and the width of the plastic film is 2cm-3 cm;
and (6): after the branches germinate, the thin film at the germinating positions is cut off, so that the germination can grow out smoothly;
and (7): grafting various fruit trees of the same genus on the same stock by adopting the grafting method from the step (1) to the step (6);
And (8): after grafting, watering, illuminating and fertilizing the stock, keeping sufficient water and fertilizer supply of the plants, timely pinching and trimming to avoid consuming excessive nutrients, removing the film after the joint is healed after grafting for 1-2 months, and simultaneously counting the survival rate of grafting after 60 days of grafting.
Two comparative examples are provided to compare with examples 1-4 of the present invention, namely comparative example 1 and comparative example 2, respectively, the scions of comparative example 1 and comparative example 2 are treated in a different manner from those of examples 1-4 before grafting, the rest of the grafting steps are the same, and the grafting survival rates of examples 1-4 and comparative examples 1-2 are shown in Table 1.
Table 1: survival rate of grafting of examples 1 to 4 and comparative examples 1 to 2
Figure BDA0003649536820000081
From table 1 it can be seen that: the survival rate of the plants grafted in the embodiments 1 to 4 is high and is not lower than 95%, the survival rate of the plants grafted in the comparative example 2 is 70% after the scions are soaked by clear water, and the survival rate of the plants grafted in the comparative example 1 is 66.7% after the scions are soaked by 40mg/L of naphthylacetic acid and 12mg/L of indoleacetic acid. Therefore, the survival rate of plant grafting can be obviously improved by soaking the auxin before scion, but the survival rate of plant grafting is reduced when the concentration of the auxin is too high.
The grafting method can produce various fruits of the same fruit tree on the same fruit tree, thus fundamentally solving the problem of single fruit variety on one fruit tree; meanwhile, the grafting method can be applied to all plants, so that the same plant flowers can be grown with the same species, the flowering phase of the plants is further prolonged, and the economic value of the plants is greatly improved.

Claims (8)

1. A grafting method of congeneric fruit tree varieties is characterized in that: the method comprises the following steps:
step (1): selecting a plant which grows robustly as a stock, and cutting the stock at a height needing grafting;
step (2): selecting semi-lignified branches or twigs without obvious insect damage of fruit trees as scions, removing terminal buds and leaves of the selected branches, and only keeping leafstalks;
and (3): beveling a cut in the middle or slightly deviated position of the rootstock;
and (4): cutting the bottom of the scion, and soaking the cut scion in auxin;
and (5): inserting the processed scion into the incision of the stock with the bud upward to ensure that the cut surface of the scion is tightly attached to the cut surface of the stock, and tightly winding and sealing the whole grafting part by using a film to ensure that the grafting part does not leak;
and (6): after the branches germinate, cutting the film at the germinating positions;
And (7): grafting various fruit trees of the same genus on the same stock by adopting the grafting method from the step (1) to the step (6);
and (8): after grafting, the stock is watered, illuminated and fertilized, and the film is removed after the joint is healed for 1-2 months.
2. The method for grafting a fruit tree variety of the same genus as defined in claim 1, wherein: in the step (1), the rootstock is a plant which is 3-4 years old and has normal growth and no plant diseases and insect pests.
3. The method for grafting the same-genus fruit tree variety as set forth in claim 2, wherein: in the step (1), the cross section of the rootstock is flat and does not damage cortex.
4. The method for grafting the same-genus fruit tree variety as set forth in claim 1, wherein: in the step (2), the branches are annual mature branches, and the length of the branches is 3cm-7 cm.
5. The method for grafting the same-genus fruit tree variety as set forth in claim 1, wherein: in the step (3), the depth of the cut is 2cm-3cm, and the cut is ensured to be flat and smooth.
6. The method for grafting the same-genus fruit tree variety as set forth in claim 1, wherein: in the step (4), the cutting surface at the bottom of the scion and the round section of the branch form an angle of 45 degrees, and the auxin is a mixture of naphthylacetic acid and indoleacetic acid.
7. The method for grafting a fruit tree variety of the same genus as defined in claim 5, wherein: in the step (4), the soaking time in auxin before scion grafting is 3-8 h, the concentration of naphthylacetic acid is 10-30 mg/L, the concentration of indoleacetic acid is 5-8 mg/L, and the mass ratio of naphthylacetic acid to indoleacetic acid is 1: 1-3.
8. The method for grafting a fruit tree variety of the same genus as defined in claim 1, wherein: in the step (5), 2 layers of films are used for winding and sealing the whole grafting part, and the films need to be disinfected before use, wherein the films are plastic films, the thickness of the plastic films is 0.003cm-0.004cm, and the width of the plastic films is 2cm-3 cm.
CN202210538811.1A 2022-05-18 2022-05-18 Grafting method for same-genus fruit tree variety Pending CN114747378A (en)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107173074A (en) * 2017-06-16 2017-09-19 国家林业局桉树研究开发中心 A kind of engrafting method of many strain windbell wood of homophyletic
CN107258447A (en) * 2017-07-21 2017-10-20 广西壮族自治区亚热带作物研究所 A kind of asexual reproduction method afforested with mango improved seeds
CN109362372A (en) * 2018-10-27 2019-02-22 韩国平 A kind of engrafting method of plant
CN110839427A (en) * 2019-12-16 2020-02-28 江苏省中国科学院植物研究所 High-stem grafting method for magnolia denudata
CN112449891A (en) * 2020-11-16 2021-03-09 广东龙飞生物有限公司 Grafting method for fruit trees

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107173074A (en) * 2017-06-16 2017-09-19 国家林业局桉树研究开发中心 A kind of engrafting method of many strain windbell wood of homophyletic
CN107258447A (en) * 2017-07-21 2017-10-20 广西壮族自治区亚热带作物研究所 A kind of asexual reproduction method afforested with mango improved seeds
CN109362372A (en) * 2018-10-27 2019-02-22 韩国平 A kind of engrafting method of plant
CN110839427A (en) * 2019-12-16 2020-02-28 江苏省中国科学院植物研究所 High-stem grafting method for magnolia denudata
CN112449891A (en) * 2020-11-16 2021-03-09 广东龙飞生物有限公司 Grafting method for fruit trees

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Application publication date: 20220715