CN107223668B - A kind of plant graft consolidant - Google Patents
A kind of plant graft consolidant Download PDFInfo
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- CN107223668B CN107223668B CN201710341229.5A CN201710341229A CN107223668B CN 107223668 B CN107223668 B CN 107223668B CN 201710341229 A CN201710341229 A CN 201710341229A CN 107223668 B CN107223668 B CN 107223668B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/08—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing solids as carriers or diluents
- A01N25/10—Macromolecular compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/16—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds containing nitrogen-to-oxygen bonds
- A01N33/18—Nitro compounds
- A01N33/20—Nitro compounds containing oxygen or sulfur attached to the carbon skeleton containing the nitro group
- A01N33/22—Nitro compounds containing oxygen or sulfur attached to the carbon skeleton containing the nitro group having at least one oxygen or sulfur atom and at least one nitro group directly attached to the same aromatic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/02—Sulfur; Selenium; Tellurium; Compounds thereof
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Abstract
Present patent application belongs to plant growth regulator technical field, a kind of plant graft consolidant, and each component mass percent concentration is as follows: sodium alginate 1%；Gelatin 2%；Calcium lactate 0.1%；1.8% compound sodium nitrophenolate 0.01~0.02%；NaHS 0.001~0.002%；50% chlorobromide isocyanuric acid 0.08~0.12%；50% humic acid 0.2~0.5%, remaining is water.The present invention can significantly improve the survival rate of grafting plant.The comprehensive graft survival rate for improving plant of the present invention, promotes young sprout growth, it is demonstrated experimentally that the present invention, than existing all kinds of graft union agent, survival rate of plant averagely improves 5% or so.
Description
Technical field
The invention belongs to plant growth regulator technical fields.
Background technique
Grafting, one of artificial nutrition propagation method of plant.Grafting is the branch or bud (i.e. scion) a kind of plant, grafting
Onto the stem or root of another plant (i.e. stock), there is after injured the function of callus using plant, make two formation for hurting face
Layer is close and tightens together, since hyperplasia is healing each other to the entirety that vascular tissue links together, finally
Grow up to a complete plant.
The factor that influence grafting survives in production practices is in addition to anvil fringe affinity, nutrition shape main or with anvil fringe
The factors such as condition, moisture, pollution, hormonal readiness, shading are related, to influence graft union healing speed and survival rate.Scion is general
It is shorter branch, an even bud, and be kept completely separate with the parent of growth, calli induction and healing are being tested
Also at least needed for 2~3 weeks under the conditions of tissue culture of room, scion early period and stock conducting tissue fail to establish connection body in time
When being, nutrition and the moisture of itself are only leaned in scion, and healing growth conditions requirement is much not achieved, if outdoor grafting, management is not
It is kind, it is allowed to exposing to the weather, arid drenches with rain, will lead to scion and dry up in 1~2 week death, grafting is difficult to be succeeded, because
This creates the good graft seedling growth environment of relative conditon and is even more important.
Therefore in modern agricultural production practice, people take many innovations also in order to improve plant graft survival rate and do
Method, it is most common be using at polyethylene film binding grafting, although having received certain effect, its shortcoming is that: due to poly-
Vinyl film is airtight, and wrapping closely causes humidity excessive very much, and wrapping does not have the effect for keeping moisture closely.So existing mesh
It is preceding also to occur a kind of plant graft consolidant that be retained on the market, for being used in combination with polyethylene film, mainly at
It point is isothiazolinone and methyl α-naphthyl acetate, it is thin that when use is applied at grafting then package polyethylene, entire to smear and the consumption of package
When it is longer, in practical effect, the survival rate of plant graft is not also significantly improved.
Summary of the invention
The invention is intended to provide a kind of plant graft consolidant that can increase substantially graft survival rate.
Technical solution: a kind of plant graft consolidant, each component mass percent concentration are as follows: sodium alginate 1%；Gelatin
2%；Calcium lactate 0.1%；1.8% compound sodium nitrophenolate 0.01~0.02%；NaHS 0.001~0.002%；50% chlorobromide isocyanuric acid
0.08~0.12%；50% humic acid 0.2~0.5%, remaining is water.
The utility model has the advantages that the sodium alginate containing 1%, gelatin 2.0% in the 1, present invention, sodium alginate and gelatin are in cream after sprinkling
Under the action of Ca2+ in sour calcium, in the presence of having the cation such as Ca2+, Sr2+, Na+ and bivalent cation on G unit occur
Ion-exchange reactions, G unit is accumulated to form cross-linked network structure, to form hydrogel, can be formed with centainly in plant surface
The firm protective film of permeability, before stock is connected to scion vascular bundle and to be formed, moisture in stock by free diffusing and
Scion is penetrated into, this protective film can prevent moisture to scatter and disappear from wound joint, scion is enabled to have sufficient moisture
Supply, keeps the activity of scion, improves the survival rate of scion, and the protective film also inhibits respiration, reduces nutrition and disappears
Consumption inhibits microorganism invasion, prevents putrid and deteriorated effect；
2, containing 0.01~0.02% 1.8% compound sodium nitrophenolate, compound sodium nitrophenolate is a kind of strength cell-activating agent, is connect with plant
It can penetrate into plant rapidly after touch, the plasm of cell is promoted to flow, improve cell viability, may advantageously facilitate grafting wound
Calli induction has good facilitation to induction anvil fringe wound calli induction；
3,50% chlorobromide isocyanuric acid containing 0.08~0.12% can be rapidly after chlorobromide isocyanuric acid is contacted with microorganism
Ground irreversibly inhibits its growth, so as to cause the death of microbial cell, has to common bacteria, fungi, virus etc. very strong
Inhibition and killing effect；Containing 0.001~0.002% NaHS, the aqueous solution that NaHS is configured to is sprayed on leaf
Face can control blade face stomatal aperture, reduce cauline leaf moisture transpiration, to have the function that reducing plant moisture scatters and disappears.
4, containing 0.2~0.5% 50% humic acid, humic acid can absorb by the root, stem and leaf of plant, promote plant raw
Root improves the respiration of plant, reduces Stoma of Leaves opening degree, reduces the transpiration of crop, adjust the activity of enzyme, such as promoted
Hydrogen oxide enzyme inhibits heteroauxin hydrogenase etc..
The present invention helps to maintain interface humidity first, and sodium alginate 1.0%, gelatin 2.0%, calcium lactate collective effect can shapes
Wound is tightly wrapped up at film, NaHS can inhibit the moisture transpiration effect of plant, reduce moisture loss, keep wet shape
State shortens callus curring time to promote callus growth；Next is beneficial to supplement the nutrition of anvil fringe, axis (branch
Bar) epidermis has the characteristic for absorbing nutrition, and a large number of nutrients that the humic acid in graft union agent contains can be supplemented directly
The nutritional need of anvil fringe growth, facilitates wound callus and is formed, and has played positive effect to high-quality callus is formed, also
Young sprout growth can be significantly facilitated.Finally, facilitating wound disinfection sterilization, avoid because of grafting failure caused by pollution, finally
The comprehensive graft survival rate for improving plant in terms of these three, promotes young sprout growth, it is demonstrated experimentally that the present invention is more each than existing
Class graft union agent, survival rate of plant at least improve 5%.
Further, each component mass percent concentration is as follows: sodium alginate 1%；Calcium lactate 0.1%；Gelatin 2%；1.8% is multiple
Nitre phenol sodium 0.01%；NaHS 0.001%；50% chlorobromide isocyanuric acid 0.08%；50% humic acid 0.2%；Remaining is water, to Solanaceae
The survival rate of plant graft is promoted most obvious.
Further, each component mass percent concentration is as follows: the sodium alginate 1%；Calcium lactate 0.1%；Gelatin 2%；
1.8% compound sodium nitrophenolate 0.02%；NaHS 0.002%；50% chlorobromide isocyanuric acid 0.12%；50% humic acid 0.5%；Remaining is water.
The survival rate of rosaceous plant grafting is promoted most obvious.
Further, each component mass percent concentration is as follows: the sodium alginate 1%；Calcium lactate 0.1%；Gelatin 2%；
1.8% compound sodium nitrophenolate 0.015%；NaHS 0.0015%；50% chlorobromide isocyanuric acid 0.1%；50% humic acid 0.35%；Remaining is
Water.Adaptability is most wide, is obviously improved to the survival rate of all kinds of plants.
Specific embodiment
Embodiment 1
A kind of plant graft consolidant, the quality of each component is as follows in every 100g:
| Component | Quality (g) |
| Sodium alginate | 1 |
| Calcium lactate | 0.1 |
| Gelatin | 2 |
| Compound sodium nitrophenolate | 0.01 |
| NaHS | 0.001 |
| 50% chlorobromide isocyanuric acid | 0.08 |
| 50% humic acid | 0.2 |
| Water | 96.609 |
Embodiment 2
A kind of plant graft consolidant, the quality of each component is as follows in every 100g:
| Component | Quality (g) |
| Sodium alginate | 1 |
| Calcium lactate | 0.1 |
| Gelatin | 2 |
| Compound sodium nitrophenolate | 0.02 |
| NaHS | 0.002 |
| 50% chlorobromide isocyanuric acid | 0.012 |
| 50% humic acid | 0.5 |
| Water | 96.366 |
Embodiment 3
A kind of plant graft consolidant, the quality of each component is as follows in every 100g:
Experimental design and result
Experiment 1: Tomato Grafting experiment
Stock uses ' osmanthus eggplant anvil 1 ', and scion is using ' Bei Jia ' for just reaching company.The sowing of the August 26 days of osmanthus eggplant anvil 1, connects
Fringe ' Bei Jia ' was sowed at August 30th.September 22 days, 4 leaf of rootstock seedling, 1 heart, when 31 heart of leaf of scion seedling, was grafted.Scion, stock exist
Ld drenches sufficient water before grafting.75% alcohol disinfecting of blade, Grafting clip and station when grafting.It is grafted using cleft graft.It transfers
Alignment processing is carried out to grafting plant immediately after connecing, experiment sets 6 processing, and the experimental subjects of each processing is that 500 plants of graftings are planted
Object.Processing 1 sprays 1 consolidant of embodiment, and processing 2 sprays 2 consolidant of embodiment, and processing 3 sprays 3 consolidant of embodiment, processing 4
It smears Sichuan Guoguang Agrochemical Co., Ltd. trees Wound-healing agent (main component acrylic acid synthetic resin), processing 5 is smeared
3% thiophanate-methyl paste of Yantai Lvyun Biochemistry Co., Ltd.'s " film founds health ", processing 6 are not processed as blank control.
After grafting seedbed covering sunshade net shading cooling, 3d starts suitably to divulge information after grafting, after gradually increase ventilation quantity
It is asked when with ventilation.Shading 5d gradually carries out light-exposed, half shading of noon sooner or later.Wound healing after 7d removes sunshade net, carries out seedbed
Normal management.20d counts survival rate after grafting, and statistical result is as follows:
| Experiment process | Survival rate |
| Processing 1 | 86.4% |
| Processing 2 | 85.9% |
| Processing 3 | 86.1% |
| Processing 4 | 80.6% |
| Processing 5 | 82.3% |
| Processing 6 | 79.2% |
The result shows that spraying consolidant of the present invention than smearing state's light trees Wound-healing agent and 3% in grafting part after grafting
Thiophanate-methyl paste significantly improves the survival rate (at least improving 5% survival rate) of grafting.And the present invention heals
Agent more saves labor compared to painting type consolidant using sprayer is spraying.
Experiment 2: loquat high-grafting experiment
Stock uses plain boiled pork type ' highest-ranking imperial concubine ' kind using life in 10 years ' liberation clock ' loquat, scion, grafts within 15 days 2 months, uses
Cut-grafting method, in trunk or the incision position of level-one branch, with longitudinal sectional 2 notch of scraper (slightly band xylem, be about 3~5cm), stock
Section knife equating.8~10cm is grown in scion, has double buds or polygerm, base portion are whittled into short inclined-plane on one side, and another side is whittled into long inclined-plane,
Long 3~5cm.Alignment forming layer is grafted when scion is inserted into, this experiment, experimental design 7 processing, each place are carried out after grafting
The experimental subjects of reason is 200 plants of grafts.Processing 1 sprays 1 consolidant of embodiment, and processing 2 sprays 2 consolidant of embodiment, locates
Reason 3 sprays 3 consolidant of embodiment, and Sichuan Guoguang Agrochemical Co., Ltd. trees Wound-healing agent (main component is smeared in processing 4
Acrylic acid synthetic resin), Yantai Lvyun Biochemistry Co., Ltd. " film founds health " 3% thiophanate-methyl paste, place are smeared in processing 5
Reason 6 is handled without sprinkling, to packet film tape at grafting after the completion of processing 1~6.Processing 7 is not wrapped after spraying 2 consolidant of embodiment
Film tape.
Use the film tape of thickness 0.01~0.02cm, wide 3cm that notch and scion bag hard-pressed bale is real after spraying processing.After 7 months
Survival rate and measurement shoot length are investigated, as a result as shown in the table:
| Experiment process | Bud survival rate | Callus forms time (d) | Shoot length (cm) |
| Processing 1 | 89.75% | 6.24 | 42.03 |
| Processing 2 | 89.81% | 6.19 | 41.86 |
| Processing 3 | 89.76% | 6.20 | 42.91 |
| Processing 4 | 83.14% | 8.14 | 36.67 |
| Processing 5 | 87.42% | 6.35 | 19.12 |
| Processing 6 | 82.75% | 8.24 | 35.54 |
| Processing 7 | 82.61% | 7.56 | 36.14 |
The result shows that the survival rate (mean value is 89% or more) of 1, processing 1~3 is apparently higher than 4~6(of processing wherein highest
Also just 87%)；Callus forms time (mean value is in 6d or so) and is significantly shorter than processing 4~6(mean value in 7d or so)；Processing 1
~3 shoot length (mean value is in 41cm or so) is considerably longer than 4~6(of processing, and wherein highest just reaches 36cm or so).Explanation is transferred
Consolidant high graft survival rate of the present invention is sprayed in grafting part after connecing, the callus formation time is short, and shoot length is big.
2, packet film tape does not spray consolidant of the present invention directly to processing 7() the survival rate packet film traditional with processing 6(
Item) survival rate is suitable, but handles plant callus in 7 and form that the time is shorter, shoot length is longer, illustrate healing of the present invention
Agent sprinkling more saves labor compared to conventional films packet item and better effect.
Embodiment 3: olive graft seedling growth experiment
Stock uses 2 years raw nutrient bag seedlings in this experiment, and scion uses Fujian Province's Putian City place olive kind ' rosy clouds
Small stream sheet ', grafting time April 15, engrafting method using single (double) bud cut-grafting method, after grafting before packet film tape grafting part into
Row processing, 6 processing of experiment point: 1 grafting part of processing sprays 1 consolidant of embodiment, and processing 2 sprays embodiment in grafting part
2 consolidants, processing 3 spray 3 consolidant of embodiment in grafting part, and Sichuan Guoguang Agrochemical Co., Ltd. tree is smeared in processing 4
The wooden Wound-healing agent (main component acrylic acid synthetic resin), Yantai Lvyun Biochemistry Co., Ltd. is smeared in processing 5, and " film is vertical
3% thiophanate-methyl paste of health ", processing 1~5 spray 150 plants of grafts.Processing 6 be choose 150 plants of grafting plants without
Any processing is as control.
Count survival rate when the long 10cm or more of most of grafting plant young sprouts after 50 days, survive strain number by the long 3cm of young sprout with
On be considered as and survive 1 plant, the long 3cm or less of young sprout is considered as 0.5 plant, and death is 0 plant, and survival rate statistical result see the table below:
| Experiment process | Survival rate |
| Processing 1 | 80.67% |
| Processing 2 | 80.19% |
| Processing 3 | 79.93% |
| Processing 4 | 74.69% |
| Processing 5 | 76.52% |
| Processing 6 | 72.33% |
For olive since tree body tissue is more loose, tannin content is higher, oxidizable in air, the grafting survival of grafting bud exposure
Rate is lower.Olive graft seedling growth the results showed that 1, handle 1~3 survival rate be apparently higher than processing 4~6, illustrate for olive
The low plant of this graft survival rate of olive, consolidant of the present invention can significantly improve its graft survival rate.
Claims (4)
1. a kind of plant graft consolidant, which is characterized in that each component mass percent concentration is as follows:
Sodium alginate 1%；
Calcium lactate 0.1%；
Gelatin 2%；
1.8% compound sodium nitrophenolate 0.01~0.02%；
NaHS 0.001~0.002%；
50% chlorobromide isocyanuric acid 0.08~0.12%；
50% humic acid 0.2~0.5%；
Remaining is water.
2. a kind of plant graft consolidant according to claim 1, which is characterized in that each component mass percent concentration is such as
Under: the sodium alginate 1%；Calcium lactate 0.1%；Gelatin 2%；1.8% compound sodium nitrophenolate 0.01%；NaHS 0.001%；50% chlorine bromine
Isocyanuric acid 0.08%；50% humic acid 0.2%；Remaining is water.
3. a kind of plant graft consolidant according to claim 1, which is characterized in that each component mass percent concentration is such as
Under: the sodium alginate 1%；Calcium lactate 0.1%；Gelatin 2%；1.8% compound sodium nitrophenolate 0.02%；NaHS 0.002%；50% chlorine bromine
Isocyanuric acid 0.12%；50% humic acid 0.5%；Remaining is water.
4. a kind of plant graft consolidant according to claim 1, which is characterized in that each component mass percent concentration is such as
Under: the sodium alginate 1%；Calcium lactate 0.1%；Gelatin 2%；1.8% compound sodium nitrophenolate 0.015%；NaHS 0.0015%；50% chlorine
Bromine isocyanuric acid 0.1%；50% humic acid 0.35%；Remaining is water.
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Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108522052A (en) * | 2018-01-30 | 2018-09-14 | 广西壮歌农业科技桑博园有限责任公司 | A method of improving mulberry sapling graft survival rate |
| CN108684351A (en) * | 2018-03-31 | 2018-10-23 | 杨奕文 | A kind of engrafting method of capsicum |
| CN108651461A (en) * | 2018-05-07 | 2018-10-16 | 合肥市晨雷思建筑材料科技有限公司 | A kind of efficient wound curing agent promoting plant healing |
| CN109819837A (en) * | 2019-04-01 | 2019-05-31 | 张为新 | A kind of dark plum grafting implantation methods |
| CN110558347A (en) * | 2019-10-14 | 2019-12-13 | 中国农业科学院郑州果树研究所 | Fruit tree grafting auxiliary agent |
| CN111406526B (en) * | 2020-04-21 | 2022-04-15 | 广西壮族自治区蚕业技术推广站 | High-position grafting rapid propagation method for mulberry |
| CN112189561A (en) * | 2020-09-29 | 2021-01-08 | 山西大学 | Application of sodium hydrosulfide as plant callus inducer |
| CN113475528B (en) * | 2021-07-02 | 2022-05-17 | 山东省林业科学研究院 | Quick healing agent for drug injection holes of pine trees |
| CN114391413B (en) * | 2022-01-27 | 2023-11-21 | 保亭智农农业发展有限责任公司 | Quick durian seedling cultivation method |
| CN117024931B (en) * | 2023-08-11 | 2024-02-13 | 云南省农业科学院茶叶研究所 | Antibacterial water-retaining gel and preparation method and application thereof |
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| CN105191725A (en) * | 2015-09-21 | 2015-12-30 | 金寨县益农农业科技开发有限公司 | High-yield sophora japonica bud carving cultivating method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105191725A (en) * | 2015-09-21 | 2015-12-30 | 金寨县益农农业科技开发有限公司 | High-yield sophora japonica bud carving cultivating method |
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