CN108522052A - A method of improving mulberry sapling graft survival rate - Google Patents
A method of improving mulberry sapling graft survival rate Download PDFInfo
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- CN108522052A CN108522052A CN201810087476.1A CN201810087476A CN108522052A CN 108522052 A CN108522052 A CN 108522052A CN 201810087476 A CN201810087476 A CN 201810087476A CN 108522052 A CN108522052 A CN 108522052A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/28—Myrtaceae [Myrtle family], e.g. teatree or clove
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
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Abstract
The present invention relates to a kind of methods improving mulberry sapling graft survival rate, belong to field of agricultural cultivation.The method for improving mulberry sapling graft survival rate, includes the following steps：(1) stock is selected：Select annual, do not grafted, adapt to locally to grow, rhizome 0.5~0.7cm mulberry sapling of growing directly from seeds as stock；(2) scion is selected：It is raw in 1 year that without being selected on disease pest, healthy and strong improved variety mulberry, luxuriant is full, enriches completely, without side shoot, thickness mock appropriate as scion；Scion is squirted in the evening before that day of grafting with clear water, fully to absorb water；(3) it grafts：Using bag connection, while scion is grafted onto stock, graft union agent is applied to around graft union, graft union is then subjected to bondage with plastic film.The survival rate of mulberry sapling grafting significantly can be increased to 95 98% by the engrafting method of the present invention, effectively shorten seedling-slowing stage, prevented nursery stock withered, promoted the growth of mulberry sapling.
Description
【Technical field】
The present invention relates to field of agricultural cultivation, and in particular to a method of improving mulberry sapling graft survival rate.
【Background technology】
Mulberry tree graft seedling growth have the characteristics that unit area nursery quantity is more, survive after mulberry sapling growing way it is strong, be adapt to it is big
Scale plants a kind of fast seedling-cultivating method and approach that mulberry needs.Currently, national " eastern Sang Xiyi " engineering is implemented steadily, future
It is several to be only this engineering every year and just need 500,000,000 plants or so of mulberry sapling.Therefore, it finds the factor that mulberry tree graft seedling growth survives that influences accurately, takes
Effective counter-measure not only improves and improves unit area nursery benefit, is also beneficial to meet national " eastern Sang Xiyi " engineering
It plants mulberry to need, moves forward steadily the smooth implementation of the engineering.
For many years, it does not solve the problem always in the mulberry tree nursery in Guangxi, mulberries are as a kind of fruit increasingly by consumer
Like, while mulberry tree be also plant Sang Jianyuan needed for nursery stock, mulberry tree rely primarily on from other places allocate and transport, since transportation range is remote, no
Only cost is high, and the easy dehydration of mulberry sapling, and survival rate is low after causing plant, while also by some quarantine Mulberry Diseases And Pests bands
Enter, in order to break away from this passive situation, greatly develops plant now and cultivate graft nursery stock, there is observation nearly 5,000,000 at present
Strain, is up to 90% or more from transferring graft seedling growth, individual plot and certain plant row survival rates according to statistics, and also some is transplanted
Survival rate is less than 30%, and the spring in 2010 takes self-fertile from the method connect, transplants 5000 plants of graft, transplants average survival
65%.According to another investigation, the nursery stock of transplanting was less than 2,100,000 plants in recent years, and average survival 41% or so, nursery survives difference
Greatly, the outstanding problem in the low work as current nursery of average survival, shows, mulberry tree graft seedling growth is related to link by analysis
More, influence factor is complicated, and by long-term observation and practice, the committed step for influencing mulberry tree graft seedling growth survival rate is being grafted
Stage can largely improve mulberry tree graft seedling growth survival rate if grafting is carried out.
Therefore, a kind of engrafting method that can improve mulberry tree graft seedling growth survival rate of searching is highly important.
【Invention content】
The goal of the invention of the present invention is：In view of the above problems, it provides and a kind of improving mulberry sapling graft survival rate
The survival rate of mulberry sapling grafting significantly can be increased to 95-98%, effectively shorten slow seedling by method, the engrafting method
Phase prevents nursery stock withered, promotes the growth of mulberry sapling.
To achieve the goals above, the technical solution adopted by the present invention is as follows：
A method of mulberry sapling graft survival rate is improved, is included the following steps：
(1) stock is selected：It selects annual, did not grafted, adapt to locally to grow, reality of the rhizome in 0.5~0.7cm
Raw mulberry sapling is as stock；
(2) scion is selected：It was given birth in 1 year without selection luxuriant is full on disease pest, healthy and strong improved variety mulberry, enriches completely, without side
Branch, thickness mock appropriate are as scion；15d cuts scion and is placed on closed, ventilation, in shady and cool, heat and moisture preserving room before grafting,
It is husky first to spread 10cm tides for ground in room, and the scion 5~10cm that tiles is thick, fill out the thick tides of 5cm it is husky after tile again 5~10cm thickness scion cycle
Tiling covers, and is closed the doors and windows with the covering of plastic foil surrounding, and temperature is kept for 5~10 DEG C, and relative humidity 75% ensures that scion is not done
It is withered, not advance germination, do not go mouldy；Scion is squirted in the evening before that day of grafting with clear water, fully to absorb water；
(3) it grafts：Using bag connection, while scion is grafted onto stock, graft union agent is applied to graft union week
It encloses, graft union is then subjected to bondage with plastic film；The graft union agent is mixed by following raw material：Cloves
8~10 parts of extracting solution, 1~3 part of Chinese blister beetle extracting solution, 2~6 parts of rhizoma arisaematis extracting solution.
Further, in step (2), the scion variety is No. 2 excellent spy, Yuesang 11, Gui Sang excellent 62.
Further, in step (3), the grafting carries out in 1 annual~April.
Further, in step (3), the cloves extracting solution is made in accordance with the following methods：It takes cloves to crush, it is full that water is added
70~80 DEG C of the n-butanol extraction of sum, filtering, filtrate recycle n-butanol, and relative density 1.10 when being then concentrated into 60 DEG C~
1.12, obtain cloves extracting solution.
Further, in step (3), the Chinese blister beetle extracting solution is made in accordance with the following methods：It takes Chinese blister beetle to crush, volume is added
Score is 50~60% ethyl alcohol, 70~80 DEG C of extractions, filtering, filtrate recycling ethanol, relative density 1.10 when being then concentrated into 60 DEG C
~1.12, obtain Chinese blister beetle extracting solution.
Further, in step (3), the rhizoma arisaematis extracting solution is made in accordance with the following methods：It takes rhizoma arisaematis to crush, is added
90~100 DEG C of extractions of water, filtering, relative density 1.10~1.12 when filtrate is concentrated into 60 DEG C obtain rhizoma arisaematis extracting solution.
In conclusion by adopting the above-described technical solution, the beneficial effects of the invention are as follows：
The method that the present invention improves mulberry sapling graft survival rate handles graft union in grafting procedures using graft union agent,
Both it can promote organization healing, significantly shorten the organization healing time, and the growth of root system can be promoted, can significantly carry
The survival rate of high grafting, effectively shortens seedling-slowing stage, prevents nursery stock withered, promotes the growth of mulberry sapling.
【Specific implementation mode】
Embodiment 1
A method of mulberry sapling graft survival rate is improved, is included the following steps：
(1) stock is selected：It selects annual, did not grafted, adapt to locally to grow, reality of the rhizome in 0.5~0.7cm
Raw mulberry sapling is as stock；
(2) scion is selected：It was given birth in 1 year without selection luxuriant is full on disease pest, healthy and strong improved variety mulberry, enriches completely, without side
Branch, thickness mock appropriate are as scion；15d cuts scion and is placed on closed, ventilation, in shady and cool, heat and moisture preserving room before grafting,
It is husky first to spread 10cm tides for ground in room, and the scion 5~10cm that tiles is thick, fill out the thick tides of 5cm it is husky after tile again 5~10cm thickness scion cycle
Tiling covers, and is closed the doors and windows with the covering of plastic foil surrounding, and temperature is kept for 5~10 DEG C, and relative humidity 75% ensures that scion is not done
It is withered, not advance germination, do not go mouldy；Scion is squirted in the evening before that day of grafting with clear water, fully to absorb water；The scion variety
It is special excellent No. 2；
(3) it grafts：It grafts and is carried out in annual March, using bag connection, while scion is grafted onto stock, grafting
Consolidant is applied to around graft union, and graft union is then carried out bondage with plastic film；The graft union agent is by following heavy
Amount part raw material mixes：9 parts of cloves extracting solution, 2 parts of Chinese blister beetle extracting solution, 4 parts of rhizoma arisaematis extracting solution；The cloves extracting solution is pressed
It is made according to following methods：It taking cloves to crush, 70~80 DEG C of extractions of water saturated n-butanol, filtering is added, filtrate recycles n-butanol,
Relative density 1.11 when being then concentrated into 60 DEG C, obtains cloves extracting solution；The Chinese blister beetle extracting solution is made in accordance with the following methods：It takes
Chinese blister beetle crushes, and it is 55% ethyl alcohol, 70~80 DEG C of extractions that volume fraction, which is added, is filtered, then filtrate recycling ethanol is concentrated into 60 DEG C
When relative density 1.11, obtain Chinese blister beetle extracting solution；The rhizoma arisaematis extracting solution is made in accordance with the following methods：Rhizoma arisaematis is taken to crush,
90~100 DEG C of extractions of water, filtering are added, relative density 1.11 when filtrate is concentrated into 60 DEG C obtains rhizoma arisaematis extracting solution.
Embodiment 2
A method of mulberry sapling graft survival rate is improved, is included the following steps：
(1) stock is selected：It selects annual, did not grafted, adapt to locally to grow, reality of the rhizome in 0.5~0.7cm
Raw mulberry sapling is as stock；
(2) scion is selected：It was given birth in 1 year without selection luxuriant is full on disease pest, healthy and strong improved variety mulberry, enriches completely, without side
Branch, thickness mock appropriate are as scion；15d cuts scion and is placed on closed, ventilation, in shady and cool, heat and moisture preserving room before grafting,
It is husky first to spread 10cm tides for ground in room, and the scion 5~10cm that tiles is thick, fill out the thick tides of 5cm it is husky after tile again 5~10cm thickness scion cycle
Tiling covers, and is closed the doors and windows with the covering of plastic foil surrounding, and temperature is kept for 5~10 DEG C, and relative humidity 75% ensures that scion is not done
It is withered, not advance germination, do not go mouldy；Scion is squirted in the evening before that day of grafting with clear water, fully to absorb water；The scion variety
For Yuesang 11；
(3) it grafts：It grafts and is carried out in annual January, using bag connection, while scion is grafted onto stock, grafting
Consolidant is applied to around graft union, and graft union is then carried out bondage with plastic film；The graft union agent is by following heavy
Amount part raw material mixes：8 parts of cloves extracting solution, 1 part of Chinese blister beetle extracting solution, 2 parts of rhizoma arisaematis extracting solution；The cloves extracting solution is pressed
It is made according to following methods：It taking cloves to crush, 70~80 DEG C of extractions of water saturated n-butanol, filtering is added, filtrate recycles n-butanol,
Relative density 1.10 when being then concentrated into 60 DEG C, obtains cloves extracting solution；The Chinese blister beetle extracting solution is made in accordance with the following methods：It takes
Chinese blister beetle crushes, and it is 50% ethyl alcohol, 70~80 DEG C of extractions that volume fraction, which is added, is filtered, then filtrate recycling ethanol is concentrated into 60 DEG C
When relative density 1.10, obtain Chinese blister beetle extracting solution；The rhizoma arisaematis extracting solution is made in accordance with the following methods：Rhizoma arisaematis is taken to crush,
90~100 DEG C of extractions of water, filtering are added, relative density 1.10 when filtrate is concentrated into 60 DEG C obtains rhizoma arisaematis extracting solution.
Embodiment 3
A method of mulberry sapling graft survival rate is improved, is included the following steps：
(1) stock is selected：It selects annual, did not grafted, adapt to locally to grow, reality of the rhizome in 0.5~0.7cm
Raw mulberry sapling is as stock；
(2) scion is selected：It was given birth in 1 year without selection luxuriant is full on disease pest, healthy and strong improved variety mulberry, enriches completely, without side
Branch, thickness mock appropriate are as scion；15d cuts scion and is placed on closed, ventilation, in shady and cool, heat and moisture preserving room before grafting,
It is husky first to spread 10cm tides for ground in room, and the scion 5~10cm that tiles is thick, fill out the thick tides of 5cm it is husky after tile again 5~10cm thickness scion cycle
Tiling covers, and is closed the doors and windows with the covering of plastic foil surrounding, and temperature is kept for 5~10 DEG C, and relative humidity 75% ensures that scion is not done
It is withered, not advance germination, do not go mouldy；Scion is squirted in the evening before that day of grafting with clear water, fully to absorb water；The scion variety
For osmanthus Sang You 62；
(3) it grafts：It grafts and is carried out in annual April, using bag connection, while scion is grafted onto stock, grafting
Consolidant is applied to around graft union, and graft union is then carried out bondage with plastic film；The graft union agent is by following heavy
Amount part raw material mixes：10 parts of cloves extracting solution, 3 parts of Chinese blister beetle extracting solution, 6 parts of rhizoma arisaematis extracting solution；The cloves extracting solution
It is made in accordance with the following methods：It takes cloves to crush, 70~80 DEG C of extractions of water saturated n-butanol, filtering is added, filtrate recycles positive fourth
Alcohol, relative density 1.12 when being then concentrated into 60 DEG C, obtains cloves extracting solution；The Chinese blister beetle extracting solution is made in accordance with the following methods
：Chinese blister beetle is taken to crush, it is 60% ethyl alcohol, 70~80 DEG C of extractions that volume fraction, which is added, is filtered, then filtrate recycling ethanol is concentrated into
Relative density 1.12 at 60 DEG C, obtains Chinese blister beetle extracting solution；The rhizoma arisaematis extracting solution is made in accordance with the following methods：Take rhizoma arisaematis powder
It is broken, 90~100 DEG C of extractions of water, filtering are added, relative density 1.12 when filtrate is concentrated into 60 DEG C obtains rhizoma arisaematis extracting solution.
Comparative example 1：
The engrafting method of comparative example 1 is substantially the same manner as Example 1, the difference is that：In the grafting step of comparative example 1,
Cloves extracting solution is replaced with clear water.
Comparative example 2：
The engrafting method of comparative example 2 is substantially the same manner as Example 1, the difference is that：In the grafting step of comparative example 2,
Chinese blister beetle extracting solution is replaced with clear water.
Comparative example 3
The engrafting method of comparative example 3 is substantially the same manner as Example 1, the difference is that, in the grafting step of comparative example 3,
The graft union agent of embodiment 1 is replaced with clear water.
Contrast test
The 1-4 months in 2017, the method that applicant uses embodiment 1-3 and comparative example 1-3 in rivers and ponds suburb mulberry sapling field
Grafting Experiment is carried out, compares mulberry sapling graft survival rate, the results are shown in Table 1：
1 graft survival rate of table compares
| Project | Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example 1 | Comparative example 2 | Comparative example 3 |
| Survival rate | 95% | 96% | 98% | 63% | 48% | 45% |
Explanation：The standard for evaluating survival rate is that graft is placed on 5~10 DEG C of Storage in cold bank, and freezer humidity is maintained at
60%~70%, after storing 14 days, the standard survived is：Stock and the healing of scion callus, fitting are close；It does not survive
Standard is：Incision epidermis thirst, and is loosened between stock and scion, dries up or black mouldy.
After engrafting method using the present invention carries out grafting processing to mulberry sapling as can be seen from the above table, mulberry sapling grafting
Survival rate is significantly improved, and improves economic benefit.
Above description is the detailed description for the present invention preferably possible embodiments, but embodiment is not limited to this hair
Bright patent claim, it is all the present invention suggested by technical spirit under completed same changes or modifications change, should all belong to
In the covered the scope of the claims of the present invention.
Claims (6)
1. a kind of method improving mulberry sapling graft survival rate, which is characterized in that include the following steps：
(1) stock is selected：It selects annual, did not grafted, adapt to locally to grow, grow directly from seeds mulberry of the rhizome in 0.5~0.7cm
Seedling is as stock；
(2) scion is selected：Given birth in 1 year without selection luxuriant is full on disease pest, healthy and strong improved variety mulberry, it is complete to enrich, without side shoot, thick
Thin mock appropriate is as scion；15d cuts scion and is placed on closed, ventilation, in shady and cool, heat and moisture preserving room before grafting, in room
It is husky that ground first spreads 10cm tides, and the scion 5~10cm that tiles is thick, fill out the thick tides of 5cm it is husky after tile again 5~10cm thickness scion cycle tiling
It covers, is closed the doors and windows with the covering of plastic foil surrounding, temperature is kept for 5~10 DEG C, and relative humidity 75% ensures that scion is not dried up, no
Advance germination is not gone mouldy；Scion is squirted in the evening before that day of grafting with clear water, fully to absorb water；
(3) it grafts：Using bag connection, while scion is grafted onto stock, graft union agent is applied to around graft union, so
Graft union is subjected to bondage with plastic film afterwards；The graft union agent is mixed by following raw material：Cloves is extracted
8~10 parts of liquid, 1~3 part of Chinese blister beetle extracting solution, 2~6 parts of rhizoma arisaematis extracting solution.
2. the method according to claim 1 for improving mulberry sapling graft survival rate, it is characterised in that：It is described to connect in step (2)
Fringe kind is No. 2 excellent spy, Yuesang 11, Gui Sang excellent 62.
3. the method according to claim 1 for improving mulberry sapling graft survival rate, it is characterised in that：It is described to transfer in step (3)
It is connected on and carries out 1 annual~April.
4. the method according to claim 1 for improving mulberry sapling graft survival rate, it is characterised in that：In step (3), the fourth
Fragrant extracting solution is made in accordance with the following methods：It takes cloves to crush, 70~80 DEG C of extractions of water saturated n-butanol, filtering, filtrate is added
N-butanol is recycled, relative density 1.10~1.12 when being then concentrated into 60 DEG C obtains cloves extracting solution.
5. the method according to claim 1 for improving mulberry sapling graft survival rate, it is characterised in that：In step (3), the spot
Chinese blister beetle extracting solution is made in accordance with the following methods：Chinese blister beetle is taken to crush, it is 50~60% ethyl alcohol, 70~80 DEG C of extractions, mistake that volume fraction, which is added,
Filter, filtrate recycling ethanol, relative density 1.10~1.12 when being then concentrated into 60 DEG C obtain Chinese blister beetle extracting solution.
6. the method according to claim 1 for improving mulberry sapling graft survival rate, it is characterised in that：In step (3), the day
Southern Star extracting solution is made in accordance with the following methods：It takes rhizoma arisaematis to crush, 90~100 DEG C of extractions of water, filtering is added, filtrate is concentrated into 60
DEG C when relative density 1.10~1.12, obtain rhizoma arisaematis extracting solution.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109258156A (en) * | 2018-10-31 | 2019-01-25 | 绵阳嫘桑缘科技有限公司 | A kind of method for culturing seedlings improving mulberry tree graft survival rate |
| CN112088670A (en) * | 2020-09-29 | 2020-12-18 | 黄昌源 | Cultivation method of vegetable mulberry variety |
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| CN107258332A (en) * | 2017-05-28 | 2017-10-20 | 陈俊豪 | The engrafting method of emperor's mandarin orange high yield |
| CN107278634A (en) * | 2017-08-11 | 2017-10-24 | 小金县夹金山清多香野生资源开发有限责任公司 | A kind of high mountain rose plant method |
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| CN102078560A (en) * | 2009-11-26 | 2011-06-01 | 贵州益佰制药股份有限公司 | Compound cantharis total extract preparation and preparation method thereof |
| CN105875206A (en) * | 2016-04-13 | 2016-08-24 | 无锡市黄土塘西瓜专业合作社 | Watermelon grafted seedling-raising method |
| CN106376360A (en) * | 2016-08-31 | 2017-02-08 | 成都新艺现代农业开发有限公司 | Grafting method for improving survival rate in raising seedlings of mulberry grafts |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109258156A (en) * | 2018-10-31 | 2019-01-25 | 绵阳嫘桑缘科技有限公司 | A kind of method for culturing seedlings improving mulberry tree graft survival rate |
| CN112088670A (en) * | 2020-09-29 | 2020-12-18 | 黄昌源 | Cultivation method of vegetable mulberry variety |
| CN112088670B (en) * | 2020-09-29 | 2022-02-08 | 黄昌源 | Cultivation method of vegetable mulberry variety |
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Application publication date: 20180914 |