CN106998787A - 通过改变硝酸同化路径减少烟草特异性亚硝胺 - Google Patents

通过改变硝酸同化路径减少烟草特异性亚硝胺 Download PDF

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Publication number
CN106998787A
CN106998787A CN201580049814.7A CN201580049814A CN106998787A CN 106998787 A CN106998787 A CN 106998787A CN 201580049814 A CN201580049814 A CN 201580049814A CN 106998787 A CN106998787 A CN 106998787A
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China
Prior art keywords
tobacco
nitrate
plant
nitrate reductase
lys
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CN201580049814.7A
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English (en)
Chinese (zh)
Inventor
鲁建立
R·S·刘易斯
R·杜威
L·博维特
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Philip Morris Products SA
North Carolina State University
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Philip Morris Products SA
North Carolina State University
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Publication of CN106998787A publication Critical patent/CN106998787A/zh
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    • AHUMAN NECESSITIES
    • A24TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24BMANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/18Treatment of tobacco products or tobacco substitutes
    • A24B15/24Treatment of tobacco products or tobacco substitutes by extraction; Tobacco extracts
    • A24B15/241Extraction of specific substances
    • A24B15/245Nitrosamines
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • AHUMAN NECESSITIES
    • A24TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24BMANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/18Treatment of tobacco products or tobacco substitutes
    • A24B15/20Biochemical treatment
    • AHUMAN NECESSITIES
    • A24TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24BMANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/18Treatment of tobacco products or tobacco substitutes
    • A24B15/24Treatment of tobacco products or tobacco substitutes by extraction; Tobacco extracts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0012Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0044Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on other nitrogen compounds as donors (1.7)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y107/00Oxidoreductases acting on other nitrogenous compounds as donors (1.7)
    • C12Y107/01Oxidoreductases acting on other nitrogenous compounds as donors (1.7) with NAD+ or NADP+ as acceptor (1.7.1)
    • C12Y107/01001Nitrate reductase (NADH) (1.7.1.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y107/00Oxidoreductases acting on other nitrogenous compounds as donors (1.7)
    • C12Y107/01Oxidoreductases acting on other nitrogenous compounds as donors (1.7) with NAD+ or NADP+ as acceptor (1.7.1)
    • C12Y107/01002Nitrate reductase [NAD(P)H] (1.7.1.2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y107/00Oxidoreductases acting on other nitrogenous compounds as donors (1.7)
    • C12Y107/01Oxidoreductases acting on other nitrogenous compounds as donors (1.7) with NAD+ or NADP+ as acceptor (1.7.1)
    • C12Y107/01003Nitrate reductase (NADPH) (1.7.1.3)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y107/00Oxidoreductases acting on other nitrogenous compounds as donors (1.7)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Nutrition Science (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Manufacture Of Tobacco Products (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Physiology (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
CN201580049814.7A 2014-09-26 2015-09-23 通过改变硝酸同化路径减少烟草特异性亚硝胺 Pending CN106998787A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP14186705 2014-09-26
EP14186705.1 2014-09-26
EP14194798.6 2014-11-25
EP14194798 2014-11-25
PCT/EP2015/071919 WO2016046288A1 (en) 2014-09-26 2015-09-23 Reducing tobacco specific nitrosamines through alteration of the nitrate assimilation pathway

Publications (1)

Publication Number Publication Date
CN106998787A true CN106998787A (zh) 2017-08-01

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CN201580049814.7A Pending CN106998787A (zh) 2014-09-26 2015-09-23 通过改变硝酸同化路径减少烟草特异性亚硝胺

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US (1) US11666082B2 (enExample)
EP (1) EP3198015A1 (enExample)
JP (1) JP6921740B2 (enExample)
KR (1) KR102524798B1 (enExample)
CN (1) CN106998787A (enExample)
BR (1) BR112017003842B1 (enExample)
CA (1) CA2959245A1 (enExample)
CO (1) CO2017004076A2 (enExample)
MX (1) MX2017003932A (enExample)
PH (1) PH12017500313A1 (enExample)
RU (1) RU2721799C2 (enExample)
WO (1) WO2016046288A1 (enExample)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108424922A (zh) * 2018-04-20 2018-08-21 云南省烟草农业科学研究院 一种降低尼古丁转化率的cyp82e10基因错义突变体m271及其应用
CN110747208A (zh) * 2019-11-28 2020-02-04 海南大学 一种木薯硝酸还原酶基因及其过量表达载体的构建和抗病应用
CN110819640A (zh) * 2019-12-19 2020-02-21 中国烟草总公司郑州烟草研究院 烟草NtNRP1基因其应用
CN116583176A (zh) * 2020-12-09 2023-08-11 日本烟草产业株式会社 烟草植物及烟草制品

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8466187B2 (en) 2007-09-18 2013-06-18 Thermolife International, Llc Amino acid compositions
CN107250148B (zh) 2014-12-03 2021-04-16 安捷伦科技有限公司 具有化学修饰的指导rna
ES2884838T3 (es) 2015-04-06 2021-12-13 Univ Leland Stanford Junior ARN guía químicamente modificados para la regulación génica mediada por CRISPR/CAS
BR112017023719A2 (pt) * 2015-05-05 2018-07-17 Univ North Carolina State planta de tabaco, parte de planta e/ou célula de planta, semente, cultura de planta, produto de tabaco, método para reduzir pon e/ou nnk, método de produção de uma planta e método para produzir um produto de tabaco
US10767175B2 (en) 2016-06-08 2020-09-08 Agilent Technologies, Inc. High specificity genome editing using chemically modified guide RNAs
CN106036984B (zh) * 2016-07-03 2017-08-08 赖高生 一种固定化蜗牛酶制备烟草浸膏的方法
CN111801423A (zh) 2018-03-28 2020-10-20 菲利普莫里斯生产公司 调节植物中的氨基酸含量
BR112020017430A2 (pt) 2018-03-28 2021-01-19 Philip Morris Products S.A. Modulação do teor de açúcar redutor em uma planta
GB201812603D0 (en) * 2018-08-02 2018-09-19 British American Tobacco Investments Ltd Method
DE102018216970A1 (de) * 2018-10-04 2020-04-09 Robert Bosch Gmbh Brennstoffeinspritzvorrichtung
CN113271768B (zh) * 2018-12-30 2024-02-06 菲利普莫里斯生产公司 经由硝酸盐还原酶的突变来调节植物中的硝酸盐水平
WO2022192743A1 (en) * 2021-03-11 2022-09-15 Thermolife International, Llc Enhanced nitrate, elemental metal, and amino acid compositions and methods of use
BR112022005552A2 (pt) 2019-10-01 2022-06-28 Philip Morris Products Sa Modulação da redução do teor de açúcar em uma planta (inv)
US12419264B2 (en) 2019-10-01 2025-09-23 Philip Morris Products S.A. Modulating sugar and amino acid content in a plant (SULTR3)
CN115516098A (zh) * 2020-01-17 2022-12-23 奥驰亚客户服务有限公司 与改进的氮利用效率有关的方法和组合物
WO2022104157A1 (en) 2020-11-12 2022-05-19 Thermolife International, Llc Methods of increasing blood oxygen saturation
US11865139B2 (en) 2020-11-12 2024-01-09 Thermolife International, Llc Method of treating migraines and headaches
IL315206A (en) 2021-02-11 2024-10-01 Thermolife Int Llc A method of administering nitric oxide gas
US11884915B2 (en) 2021-09-10 2024-01-30 Agilent Technologies, Inc. Guide RNAs with chemical modification for prime editing
WO2023117661A1 (en) 2021-12-20 2023-06-29 Philip Morris Products S.A. Increasing anatabine in tobacco leaf by regulating methyl putrescine oxidase
WO2023117701A1 (en) 2021-12-21 2023-06-29 Philip Morris Products S.A. Modulation of nicotine production by alteration of nicotinamidase expression or function in plants
US20240132900A1 (en) * 2022-02-24 2024-04-25 University Of Florida Research Foundation, Incorporated T-DNA Free Gene Editing through Transient Suppressing POLQ in Plants
JP2025533986A (ja) 2022-10-13 2025-10-09 フィリップ・モーリス・プロダクツ・ソシエテ・アノニム Ntp2を調節することによって葉バイオマスおよび窒素利用効率を増加させる
KR20250139371A (ko) 2023-02-02 2025-09-23 필립모리스 프로덕츠 에스.에이. 당 수송체의 조절
CN120476136A (zh) 2023-02-02 2025-08-12 菲利普莫里斯生产公司 编码赖氨酸酮戊二酸还原酶的基因的调节
WO2025003105A1 (en) 2023-06-29 2025-01-02 Philip Morris Products S.A. Modulation of genes coding for glutamate dehydrogenase

Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1471577A (zh) * 2000-08-30 2004-01-28 �Ϻ���ͨ��ѧ 含有可改变蛋白含量的分子诱导物的转基因植物
CN1638655A (zh) * 2001-06-08 2005-07-13 韦克多烟草有限公司 修饰烟草中烟碱和亚硝胺的水平
CN1838878A (zh) * 2003-08-19 2006-09-27 二十二世纪有限公司 低危害的烟草产品
CN1926235A (zh) * 2003-10-16 2007-03-07 美国无烟烟草公司 从烟草克隆细胞色素p450基因
WO2008115487A1 (en) * 2007-03-16 2008-09-25 New York University Methods of affecting plant growth with microrna
CN101939431A (zh) * 2007-11-12 2011-01-05 北卡罗来纳州立大学 通过修饰特定细胞色素p450基因改变烟草生物碱含量
EP2537929A1 (en) * 2010-02-17 2012-12-26 Japan Tobacco, Inc. Plant component regulation factor, and use thereof
CN102858983A (zh) * 2010-01-15 2013-01-02 北卡罗来纳州立大学 最小化烟草中去甲烟碱合成的组合物和方法
CN103315384A (zh) * 2007-02-23 2013-09-25 美国无烟烟草有限责任公司 新型烟草组合物及制备方法
CN103403021A (zh) * 2010-09-22 2013-11-20 英美烟草(投资)有限公司 转基因植物
CN103993023A (zh) * 2006-12-15 2014-08-20 美国无烟烟草有限责任公司 烟碱脱甲基酶活性降低的烟草植物

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US5990387A (en) 1988-06-10 1999-11-23 Pioneer Hi-Bred International, Inc. Stable transformation of plant cells
US5879918A (en) 1989-05-12 1999-03-09 Pioneer Hi-Bred International, Inc. Pretreatment of microprojectiles prior to using in a particle gun
US5932782A (en) 1990-11-14 1999-08-03 Pioneer Hi-Bred International, Inc. Plant transformation method using agrobacterium species adhered to microprojectiles
EP0652965A1 (en) 1992-07-27 1995-05-17 Pioneer Hi-Bred International, Inc. An improved method of agrobacterium-mediated transformation of cultured soybean cells
US5981840A (en) 1997-01-24 1999-11-09 Pioneer Hi-Bred International, Inc. Methods for agrobacterium-mediated transformation
RU2257711C2 (ru) * 2003-10-06 2005-08-10 Всероссийский научно-исследовательский институт защиты растений Композиция на основе хитозана, снижающая содержание нитратов в растениеводческой продукции
EP2087123B1 (en) 2006-10-13 2011-08-10 North Carolina State University Alteration of tobacco alkaloid content through modification of specific cytochrome p450 genes
RU2324737C1 (ru) * 2006-10-18 2008-05-20 Институт цитологии и генетики Сибирского отделения Российской академии наук (СО РАН) Способ получения трансгенных растений табака с повышенным содержанием пролина
BR112013021515A2 (pt) 2011-02-28 2018-06-12 Univ North Carolina State plantas congênitas de tabaco ncbex1f, ncbex1ms e nc ex90
GB201204869D0 (en) * 2012-03-20 2012-05-02 Cambridge Advanced Tech Transgenic plants
ITMI20121419A1 (it) * 2012-08-08 2014-02-09 Fattoria Autonoma Tabacchi S C A R L Metodo per la produzione di tabacco volto a ridurre il tenore di nitrosammine.

Patent Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1471577A (zh) * 2000-08-30 2004-01-28 �Ϻ���ͨ��ѧ 含有可改变蛋白含量的分子诱导物的转基因植物
CN1638655A (zh) * 2001-06-08 2005-07-13 韦克多烟草有限公司 修饰烟草中烟碱和亚硝胺的水平
CN1838878A (zh) * 2003-08-19 2006-09-27 二十二世纪有限公司 低危害的烟草产品
CN1926235A (zh) * 2003-10-16 2007-03-07 美国无烟烟草公司 从烟草克隆细胞色素p450基因
CN103993023A (zh) * 2006-12-15 2014-08-20 美国无烟烟草有限责任公司 烟碱脱甲基酶活性降低的烟草植物
CN103315384A (zh) * 2007-02-23 2013-09-25 美国无烟烟草有限责任公司 新型烟草组合物及制备方法
WO2008115487A1 (en) * 2007-03-16 2008-09-25 New York University Methods of affecting plant growth with microrna
CN101939431A (zh) * 2007-11-12 2011-01-05 北卡罗来纳州立大学 通过修饰特定细胞色素p450基因改变烟草生物碱含量
CN102858983A (zh) * 2010-01-15 2013-01-02 北卡罗来纳州立大学 最小化烟草中去甲烟碱合成的组合物和方法
EP2537929A1 (en) * 2010-02-17 2012-12-26 Japan Tobacco, Inc. Plant component regulation factor, and use thereof
CN103403021A (zh) * 2010-09-22 2013-11-20 英美烟草(投资)有限公司 转基因植物

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
CATHRINE LILLO等: "Mechanism and importance of post-translational regulation of nitrate reductase", 《JOURNAL OF EXPERIMENTAL BOTANY》 *
CATHRINE LILLO等: "Mutation of the regulatory phosphorylation site of tobacco nitrate reductase results in constitutive activation of the enzyme in vivo and nitrite accumulation", 《THE PLANT JOUNAL》 *
UNNI S LEA等: "Posttranslational Regulation of Nitrate Reductase Strongly Affects the Levels of Free Amino Acids and Nitrate", 《PLANT PHYSIOLOGY》 *
UNNI S LEA等: "硝酸还原酶通过翻译调控对游离氨基酸和硝酸盐水平影响较强、而通过转录调节只有微弱影响", 《植物生理学》 *
VAUCHERET H. 等: "Nitrate reductase [NADH] 2", 《UNIPROT DATABASE》 *
罗云波: "《食品生物技术导论》", 30 August 2011, 中国农业大学出版社 *
金平正等: "《卷烟烟气安全性与危害防范》", 30 September 2009, 中国轻工业出版社 *

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108424922A (zh) * 2018-04-20 2018-08-21 云南省烟草农业科学研究院 一种降低尼古丁转化率的cyp82e10基因错义突变体m271及其应用
CN108424922B (zh) * 2018-04-20 2021-04-16 云南省烟草农业科学研究院 一种降低尼古丁转化率的cyp82e10基因错义突变体m271及其应用
CN110747208A (zh) * 2019-11-28 2020-02-04 海南大学 一种木薯硝酸还原酶基因及其过量表达载体的构建和抗病应用
CN110747208B (zh) * 2019-11-28 2022-04-22 海南大学 一种木薯硝酸还原酶基因及其过量表达载体的构建和抗病应用
CN110819640A (zh) * 2019-12-19 2020-02-21 中国烟草总公司郑州烟草研究院 烟草NtNRP1基因其应用
CN110819640B (zh) * 2019-12-19 2022-06-21 中国烟草总公司郑州烟草研究院 烟草NtNRP1基因其应用
CN116583176A (zh) * 2020-12-09 2023-08-11 日本烟草产业株式会社 烟草植物及烟草制品
CN116583176B (zh) * 2020-12-09 2024-12-31 日本烟草产业株式会社 烟草植物及烟草制品

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RU2017114356A (ru) 2018-10-29
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CA2959245A1 (en) 2016-03-31
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