BR112022005552A2 - Modulação da redução do teor de açúcar em uma planta (inv) - Google Patents

Modulação da redução do teor de açúcar em uma planta (inv)

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Publication number
BR112022005552A2
BR112022005552A2 BR112022005552A BR112022005552A BR112022005552A2 BR 112022005552 A2 BR112022005552 A2 BR 112022005552A2 BR 112022005552 A BR112022005552 A BR 112022005552A BR 112022005552 A BR112022005552 A BR 112022005552A BR 112022005552 A2 BR112022005552 A2 BR 112022005552A2
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BR
Brazil
Prior art keywords
polynucleotide
ntinv4
seq
plant
inv
Prior art date
Application number
BR112022005552A
Other languages
English (en)
Inventor
Bovet Lucien
Hilfiker Aurore
Original Assignee
Philip Morris Products Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Philip Morris Products Sa filed Critical Philip Morris Products Sa
Publication of BR112022005552A2 publication Critical patent/BR112022005552A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C12N15/8246—Non-starch polysaccharides, e.g. cellulose, fructans, levans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2431—Beta-fructofuranosidase (3.2.1.26), i.e. invertase
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/82—Solanaceae, e.g. pepper, tobacco, potato, tomato or eggplant
    • A01H6/823—Nicotiana, e.g. tobacco
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/18—Treatment of tobacco products or tobacco substitutes
    • A24B15/24—Treatment of tobacco products or tobacco substitutes by extraction; Tobacco extracts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/1062—Sucrose synthase (2.4.1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01013—Sucrose synthase (2.4.1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01026—Beta-fructofuranosidase (3.2.1.26), i.e. invertase

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Botany (AREA)
  • Physiology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Manufacture Of Tobacco Products (AREA)

Abstract

MODULAÇÃO DA REDUÇÃO DO TEOR DE AÇÚCAR EM UMA PLANTA (INV). A presente invenção refere-se a uma célula vegetal compreendendo: (i) um polinucleotídeo compreendendo, consistindo ou consistindo essencialmente em uma sequência com pelo menos 81% de identidade de sequência com a SEQ ID NO: 5 (NtINV4-S) ou pelo menos 62% de identidade de sequência com a SEQ ID NO: 7 (NtINV4-T); (ii) um polipeptídeo codificado pelo polinucleotídeo apresentado em (i); (iii) um polipeptídeo compreendendo, consistindo ou consistindo essencialmente em uma sequência com pelo menos 85% de identidade de sequência com a SEQ ID NO: 6 (NtINV4-S) ou pelo menos 85% de identidade de sequência com a SEQ ID NO: 8 (NtINV4-T); ou (iv) um construto, vetor ou vetor de expressão compreendendo o polinucleotídeo isolado estabelecido em (i), em que a referida célula vegetal compreende pelo menos uma modificação que modula (a) a expressão ou atividade do polinucleotídeo ou (b) a expressão ou atividade do polinucleotídeo o polipeptídeo, em comparação com uma célula vegetal de controle na qual a expressão ou atividade do polinucleotídeo ou polipeptídeo não foi modificada.
BR112022005552A 2019-10-01 2020-09-28 Modulação da redução do teor de açúcar em uma planta (inv) BR112022005552A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP19200865 2019-10-01
PCT/EP2020/077048 WO2021063860A1 (en) 2019-10-01 2020-09-28 Modulating reducing sugar content in a plant (inv)

Publications (1)

Publication Number Publication Date
BR112022005552A2 true BR112022005552A2 (pt) 2022-06-28

Family

ID=68136164

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112022005552A BR112022005552A2 (pt) 2019-10-01 2020-09-28 Modulação da redução do teor de açúcar em uma planta (inv)

Country Status (7)

Country Link
US (1) US20220348944A1 (pt)
EP (1) EP4041896A1 (pt)
JP (1) JP2022550383A (pt)
KR (1) KR20220070305A (pt)
CN (1) CN114829587A (pt)
BR (1) BR112022005552A2 (pt)
WO (1) WO2021063860A1 (pt)

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CN1618976A (zh) * 2004-07-13 2005-05-25 甘肃农业大学 导入AcInv反义基因培育抗低温糖化马铃薯品系的方法
WO2006091194A1 (en) 2005-02-23 2006-08-31 North Carolina State University Alteration of tobacco alkaloid content through modification of specific cytochrome p450 genes
AU2006294680A1 (en) * 2005-09-27 2007-04-05 Cornell University Invertase and inhibitors from coffee
BRPI0717355B1 (pt) 2006-10-13 2018-01-16 North Carolina State University Método para obtenção de uma planta transgênica de nicotina, método para obtenção de uma semente; construto de ácido nucléico recombinante; método de redução da conversão de nicotina em nornicotina em uma planta de nicotina
BRPI0820042B1 (pt) 2007-11-12 2020-05-19 North Carolina State University método de obtenção de uma planta de tabaco, ou célula ou parte desta, tendo níveis reduzidos de nornicotina, produto de tabaco, método para fabricar um produto de tabaco, polinucleotídeo isolado, cassete de expressão, polipetpídeo isolado e método de obtenção de uma planta, ou parte de planta desta, do gênero nicotiana
DK2231861T3 (en) 2007-12-13 2015-01-19 Philip Morris Products Sa Transgenic plants modified to produce less cadmium transport, derivative products and related methods.
DE102008064184A1 (de) * 2008-12-22 2010-08-12 Südzucker AG Mannheim/Ochsenfurt Verfahren zur Steigerung des Saccharoseertrages beim landwirtschaftlichen Anbau von Zuckerrüben und Zuckerrohr
CA2748767A1 (en) * 2009-02-03 2010-08-12 Wisconsin Alumni Research Foundation Control of cold-induced sweetening and reduction of acrylamide levels in potato or sweet potato
CN102858983B (zh) 2010-01-15 2015-08-12 北卡罗来纳州立大学 最小化烟草中去甲烟碱合成的组合物和方法
EP3447065A1 (en) 2010-09-03 2019-02-27 Philip Morris Products S.A. Heavy metal reduction in planta
EP2565265A1 (en) 2011-09-02 2013-03-06 Philip Morris Products S.A. Isopropylmalate synthase from Nicotiana tabacum and methods and uses thereof
EP2565271A1 (en) 2011-09-02 2013-03-06 Philip Morris Products S.A. Threonine synthase from Nicotiana tabacum and methods and uses thereof
AU2012331235B2 (en) 2011-10-31 2017-06-15 Philip Morris Products S.A. Modulating beta-damascenone in plants
CA2894955C (en) 2012-12-21 2023-10-31 Philip Morris Products S.A. Tobacco specific nitrosamine reduction in plants
US20140178561A1 (en) * 2012-12-21 2014-06-26 Cellectis Potatoes with reduced cold-induced sweetening
AU2015213254A1 (en) * 2014-01-29 2017-08-17 The University Of Queensland Yield promoter to increase sucrose and sucrose derivatives in plants
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KR20170032317A (ko) 2014-07-18 2017-03-22 필립모리스 프로덕츠 에스.에이. 담배 프로테아제 유전자
KR102524798B1 (ko) 2014-09-26 2023-04-24 필립모리스 프로덕츠 에스.에이. 니트레이트 동화 경로의 변경을 통한 담배 특이적 니트로소아민의 감소
CA2998286C (en) 2015-09-09 2023-11-07 Philip Morris Products S.A. Plants with reduced asparagine content
MX2018009002A (es) 2016-01-29 2018-11-19 Philip Morris Products Sa Reduccion de la acumulacion de cadmio en plantas cultivadas en el campo.
CN105686070A (zh) * 2016-04-28 2016-06-22 湖南中烟工业有限责任公司 一种烟草源基础烟味原料的制备方法
US11685929B2 (en) 2016-12-20 2023-06-27 Philip Morris Products S.A. Plants with shortened time to flowering
EP3480314A1 (en) 2017-11-03 2019-05-08 Philip Morris Products S.A. Regulation of alkaloid content

Also Published As

Publication number Publication date
US20220348944A1 (en) 2022-11-03
EP4041896A1 (en) 2022-08-17
CN114829587A (zh) 2022-07-29
KR20220070305A (ko) 2022-05-30
WO2021063860A1 (en) 2021-04-08
JP2022550383A (ja) 2022-12-01

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