BR112018002914A2 - plantas com teor reduzido de asparagina - Google Patents

plantas com teor reduzido de asparagina

Info

Publication number
BR112018002914A2
BR112018002914A2 BR112018002914A BR112018002914A BR112018002914A2 BR 112018002914 A2 BR112018002914 A2 BR 112018002914A2 BR 112018002914 A BR112018002914 A BR 112018002914A BR 112018002914 A BR112018002914 A BR 112018002914A BR 112018002914 A2 BR112018002914 A2 BR 112018002914A2
Authority
BR
Brazil
Prior art keywords
sequence
seq
plants
asparagine synthetase
reduced
Prior art date
Application number
BR112018002914A
Other languages
English (en)
Inventor
Bovet Lucien
Original Assignee
Philip Morris Products Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Philip Morris Products Sa filed Critical Philip Morris Products Sa
Publication of BR112018002914A2 publication Critical patent/BR112018002914A2/pt

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/12—Leaves
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/18—Treatment of tobacco products or tobacco substitutes
    • A24B15/20—Biochemical treatment
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B3/00—Preparing tobacco in the factory
    • A24B3/12—Steaming, curing, or flavouring tobacco
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
    • C12Y603/05—Carbon-nitrogen ligases with glutamine as amido-N-donor (6.3.5)
    • C12Y603/05004—Asparagine synthase (glutamine-hydrolyzing) (6.3.5.4)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Physiology (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Manufacture Of Tobacco Products (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Fertilizers (AREA)
  • Peptides Or Proteins (AREA)

Abstract

é descrita neste documento uma planta mutante, de ocorrência não natural ou transgênica ou parte da mesma com a expressão ou atividade reduzida da asparagina sintetase, a referida asparagina sintetase compreendendo, consistindo em ou consistindo essencialmente em: (i) uma sequência de polinucleotídeo compreendendo, consistindo em ou consistindo essencialmente em uma sequência que tem pelo menos 90% da identidade de sequência para seq id no: 1 ou pelo menos 72% da identidade de sequência para seq id no: 3 ou seq id no: 5 ou seq id no: 7; ou (ii) um polipeptídeo codificado pelo polinucleotídeo apresentado em (i); ou (iii) um polipeptídeo que tem, pelo menos, 78% de identidade de sequência para seq id no: 2 ou seq id no: 4 ou seq id no: 6 ou seq id no: 8; em que a expressão ou atividade da asparagina sintetase apresentada em (i), (ii) ou (iii) é reduzida em comparação a uma planta de controle.
BR112018002914A 2015-09-09 2016-09-06 plantas com teor reduzido de asparagina BR112018002914A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP15184528 2015-09-09
PCT/EP2016/070972 WO2017042162A1 (en) 2015-09-09 2016-09-06 Plants with reduced asparagine content

Publications (1)

Publication Number Publication Date
BR112018002914A2 true BR112018002914A2 (pt) 2018-11-06

Family

ID=54105683

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112018002914A BR112018002914A2 (pt) 2015-09-09 2016-09-06 plantas com teor reduzido de asparagina

Country Status (19)

Country Link
US (1) US10883114B2 (pt)
EP (1) EP3346826A1 (pt)
JP (1) JP6810134B2 (pt)
KR (1) KR102207550B1 (pt)
CN (1) CN107920489A (pt)
AR (1) AR105968A1 (pt)
AU (1) AU2016318423B2 (pt)
BR (1) BR112018002914A2 (pt)
CA (1) CA2998286C (pt)
HK (1) HK1255341A1 (pt)
IL (1) IL257305B (pt)
MX (1) MX2018002695A (pt)
MY (1) MY187046A (pt)
NZ (1) NZ738897A (pt)
PH (1) PH12017502292A1 (pt)
RU (1) RU2742725C2 (pt)
UA (1) UA125478C2 (pt)
WO (1) WO2017042162A1 (pt)
ZA (1) ZA201708411B (pt)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3775224A1 (en) 2018-03-28 2021-02-17 Philip Morris Products S.a.s. Modulating amino acid content in a plant
EP3772911A1 (en) 2018-03-28 2021-02-17 Philip Morris Products S.a.s. Modulating reducing sugar content in a plant
EP3902390A1 (en) 2018-12-30 2021-11-03 Philip Morris Products S.A. Modulation of nitrate levels in plants via mutation of nitrate reductase
JP2022550383A (ja) 2019-10-01 2022-12-01 フィリップ・モーリス・プロダクツ・ソシエテ・アノニム 植物における還元糖含有量の調節（ｉｎｖ）
KR20220071260A (ko) 2019-10-01 2022-05-31 필립모리스 프로덕츠 에스.에이. 식물 내 당 및 아미노산 (sultr3) 함량 조절
CN112931182B (zh) * 2021-01-29 2022-06-07 四川农业大学 一种提高小麦染色体重组频率的方法
WO2023036691A1 (en) 2021-09-10 2023-03-16 Philip Morris Products S.A. Modulating alkaloid profiles in nicotiana tabacum
WO2023117661A1 (en) 2021-12-20 2023-06-29 Philip Morris Products S.A. Increasing anatabine in tobacco leaf by regulating methyl putrescine oxidase
WO2023117701A1 (en) 2021-12-21 2023-06-29 Philip Morris Products S.A. Modulation of nicotine production by alteration of nicotinamidase expression or function in plants
CN114766359B (zh) * 2022-04-22 2023-03-31 华中农业大学 一种试管苗支架及其在柑橘茎尖微芽嫁接脱毒中的应用
WO2024079137A1 (en) 2022-10-13 2024-04-18 Philip Morris Products S.A. Increasing leaf biomass and nitrogen use efficiency by regulating ntp2

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2006292286B2 (en) 2005-09-20 2013-02-14 J.R. Simplot Company Low acrylamide foods
AU2013200664B2 (en) 2005-09-20 2015-04-30 J.R. Simplot Company Low acrylamide foods
RU2324737C1 (ru) * 2006-10-18 2008-05-20 Институт цитологии и генетики Сибирского отделения Российской академии наук (СО РАН) Способ получения трансгенных растений табака с повышенным содержанием пролина
JP4953808B2 (ja) 2006-12-29 2012-06-13 フルタ電機株式会社 葉たばこの監視制御盤の制御盤により制御する乾燥方法
US8952218B2 (en) * 2008-12-29 2015-02-10 Evogene Ltd. Polynucleotides, polypeptides encoded thereby, and methods of using same for increasing abiotic stress tolerance, biomass and/or yield in plants expressing same
KR101214690B1 (ko) 2010-10-15 2012-12-21 고려대학교 산학협력단 병저항성 관련 고추 아스파라진합성효소 유전자 ＣａＡＳ１ 및 이를 이용한 식물체의 병저항성 탐색 방법
CN102121018A (zh) 2010-12-22 2011-07-13 上海大学 一种具有天冬酰胺合成酶功能的基因、其编码蛋白及其应用
US8716571B2 (en) * 2011-09-21 2014-05-06 Reynolds Technologies, Inc. Tobacco having reduced amounts of amino acids and methods for producing such lines
CN104168759B (zh) 2011-09-21 2020-01-14 雷诺兹科技股份有限公司 具有减少量的氨基酸的烟草和用于制造这种品系的方法
US20140033248A1 (en) 2012-07-27 2014-01-30 General Instrument Corporation Including inserts in multimedia content
JP2015534834A (ja) 2012-11-20 2015-12-07 ジェイ．アール．シンプロット カンパニー Ｔａｌにより媒介されるトランファーｄｎａ挿入
US20140363561A1 (en) 2013-03-15 2014-12-11 J.R. Simplot Company Tal-mediated transfer dna insertion

Also Published As

Publication number Publication date
IL257305B (en) 2022-06-01
KR102207550B1 (ko) 2021-01-27
NZ738897A (en) 2019-10-25
UA125478C2 (uk) 2022-03-30
US20190169628A1 (en) 2019-06-06
JP2018527934A (ja) 2018-09-27
CN107920489A (zh) 2018-04-17
WO2017042162A1 (en) 2017-03-16
HK1255341A1 (zh) 2019-08-16
KR20180051530A (ko) 2018-05-16
PH12017502292A1 (en) 2018-06-25
CA2998286C (en) 2023-11-07
ZA201708411B (en) 2019-01-30
EP3346826A1 (en) 2018-07-18
RU2018112407A3 (pt) 2020-02-25
AR105968A1 (es) 2017-11-29
RU2742725C2 (ru) 2021-02-10
US10883114B2 (en) 2021-01-05
IL257305A (en) 2018-03-29
RU2018112407A (ru) 2019-10-09
AU2016318423A1 (en) 2018-04-05
CA2998286A1 (en) 2017-03-16
MY187046A (en) 2021-08-27
AU2016318423B2 (en) 2022-01-20
JP6810134B2 (ja) 2021-01-06
MX2018002695A (es) 2018-04-13

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Legal Events

Date Code Title Description
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]

Free format text: MANTIDO O INDEFERIMENTO UMA VEZ QUE NAO FOI APRESENTADO RECURSO DENTRO DO PRAZO LEGAL.MANTIDO O INDEFERIMENTO UMA VEZ QUE NAO FOI APRESENTADO RECURSO DENTRO DO PRAZO LEGAL.