BR112018014765A2 - redução do acúmulo de cádmio em plantas de tabaco cultivadas em campo - Google Patents

redução do acúmulo de cádmio em plantas de tabaco cultivadas em campo

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Publication number
BR112018014765A2
BR112018014765A2 BR112018014765A BR112018014765A BR112018014765A2 BR 112018014765 A2 BR112018014765 A2 BR 112018014765A2 BR 112018014765 A BR112018014765 A BR 112018014765A BR 112018014765 A BR112018014765 A BR 112018014765A BR 112018014765 A2 BR112018014765 A2 BR 112018014765A2
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BR
Brazil
Prior art keywords
hmas
sequence
field
iii
activity
Prior art date
Application number
BR112018014765A
Other languages
English (en)
Inventor
Bovet Lucien
Liedschulte Verena
Original Assignee
Philip Morris Products Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Philip Morris Products Sa filed Critical Philip Morris Products Sa
Publication of BR112018014765A2 publication Critical patent/BR112018014765A2/pt

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/45Tobacco
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/12Leaves
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/82Solanaceae, e.g. pepper, tobacco, potato, tomato or eggplant
    • A01H6/823Nicotiana, e.g. tobacco
    • AHUMAN NECESSITIES
    • A24TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24BMANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/10Chemical features of tobacco products or tobacco substitutes
    • AHUMAN NECESSITIES
    • A24TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24BMANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/18Treatment of tobacco products or tobacco substitutes
    • A24B15/24Treatment of tobacco products or tobacco substitutes by extraction; Tobacco extracts
    • A24B15/241Extraction of specific substances
    • A24B15/248Heavy metals
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Botany (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Physiology (AREA)
  • Virology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

a presente invenção refere-se uma planta mutante ou parte da mesma com expressão ou atividade, pelo menos parcialmente reduzida, de pelo menos duas atpases de metais pesados (hmas), em que as referidas duas hmas compreendem, consistem ou consistem essencialmente em: (i) polipeptídeos com pelo menos 65% de identidade de sequência com a seq id no:1 e seq id no:2; (ii) polinucleotídeos que codificam os polipeptídeos estabelecidos em (i); ou (iii) polinucleotídeos com pelo menos 65% de identidade de sequência com a seq id no:3 e seq id no:4 codificando hmas; em que a expressão ou atividade de uma das hmas estabelecidas em (i) ou (ii) ou (iii) é parcialmente reduzida ou perdida e a expressão ou atividade de uma das hmas estabelecidas em (i) ou (ii) ou (iii) é perdida em comparação com uma planta controle; e em que a planta mutante ou parte dela apresenta pelo menos uma redução de 27% em comparação com a planta controle no acúmulo de cádmio na folha quando a planta mutante é cultivada no campo na presença de cádmio de ocorrência natural ou não natural.
BR112018014765A 2016-01-29 2017-01-27 redução do acúmulo de cádmio em plantas de tabaco cultivadas em campo BR112018014765A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP16153529 2016-01-29
PCT/EP2017/051761 WO2017129739A1 (en) 2016-01-29 2017-01-27 Reducing cadmium accumulation in field grown tobacco plants

Publications (1)

Publication Number Publication Date
BR112018014765A2 true BR112018014765A2 (pt) 2018-12-26

Family

ID=55359387

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112018014765A BR112018014765A2 (pt) 2016-01-29 2017-01-27 redução do acúmulo de cádmio em plantas de tabaco cultivadas em campo

Country Status (14)

Country Link
US (1) US11213004B2 (pt)
EP (1) EP3407704B1 (pt)
JP (1) JP7129335B2 (pt)
KR (1) KR20180107123A (pt)
CN (1) CN108697059B (pt)
AR (1) AR107478A1 (pt)
AU (1) AU2017211970A1 (pt)
BR (1) BR112018014765A2 (pt)
CA (1) CA3010142A1 (pt)
CO (1) CO2018007797A2 (pt)
EA (1) EA201891707A1 (pt)
MX (1) MX2018009002A (pt)
PH (1) PH12018501304A1 (pt)
WO (1) WO2017129739A1 (pt)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108157104B (zh) * 2017-12-12 2020-08-04 贵州省烟草公司黔东南州公司 一种蚜茧蜂保种和扩种用烟株的培育方法
BR112020017430A2 (pt) 2018-03-28 2021-01-19 Philip Morris Products S.A. Modulação do teor de açúcar redutor em uma planta
AR115022A1 (es) 2018-03-28 2020-11-18 Philip Morris Products Sa Modulación del contenido de aminoácidos en una planta
MX2021007628A (es) 2018-12-30 2021-08-11 Philip Morris Products Sa Modulacion de niveles de nitrato en plantas a traves de la mutacion de la nitrato reductasa.
CN114829587A (zh) 2019-10-01 2022-07-29 菲利普莫里斯生产公司 调节植物中的还原糖含量(inv)
US20230200344A1 (en) 2019-10-01 2023-06-29 Philip Morris Products S.A. Modulating sugar and amino acid content in a plant (sultr3)
CN111436343A (zh) * 2019-12-19 2020-07-24 中国农业科学院烟草研究所 一种青枯病鉴定方法
CN111727784A (zh) * 2020-07-27 2020-10-02 贵州省烟草科学研究院 一种烟草黑胫病始发期的预报方法
CN114736908B (zh) * 2020-12-23 2024-02-09 中国科学院分子植物科学卓越创新中心 调节植物镉含量以及镉耐受性的基因及其应用
CN114451256B (zh) * 2021-11-15 2023-07-04 玉溪中烟种子有限责任公司 一种基质处理方法及使用该基质的烟草种子育苗方法
WO2023117661A1 (en) 2021-12-20 2023-06-29 Philip Morris Products S.A. Increasing anatabine in tobacco leaf by regulating methyl putrescine oxidase
WO2023117701A1 (en) 2021-12-21 2023-06-29 Philip Morris Products S.A. Modulation of nicotine production by alteration of nicotinamidase expression or function in plants
WO2024079137A1 (en) 2022-10-13 2024-04-18 Philip Morris Products S.A. Increasing leaf biomass and nitrogen use efficiency by regulating ntp2
WO2024160864A1 (en) 2023-02-02 2024-08-08 Philip Morris Products S.A. Modulation of sugar transporters
WO2024160860A1 (en) 2023-02-02 2024-08-08 Philip Morris Products S.A. Modulation of genes coding for lysine ketoglutarate reductase

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE530656T1 (de) 2005-02-23 2011-11-15 Univ North Carolina State Veränderung des alkaloidgehaltes in tabak durch modifikation spezifischer cytochrome p450 gene.
CA2666383C (en) 2006-10-13 2015-12-22 North Carolina State University Alteration of tobacco alkaloid content through modification of specific cytochrome p450 genes
EP2220231B1 (en) 2007-11-12 2017-10-11 North Carolina State University Alteration of tobacco alkaloid content through modification of specific cytochrome p450 genes
US20110023177A1 (en) 2007-12-12 2011-01-27 Pierre Richaud Genetically Modified Plants Displaying Reduced Accumulation of Cadmium
EP2231861B1 (en) 2007-12-13 2014-10-29 Philip Morris Products S.A. Transgenic plants modified for reduced cadmium transport, derivative products, and related methods
EA033565B1 (ru) 2010-01-15 2019-11-05 Univ North Carolina State Композиции и способы, предназначенные для минимизации синтеза норникотина в растениях табака
BR112013005142A2 (pt) * 2010-09-03 2020-08-04 Philip Morris Products S.A. redução de metal pesado em planta
ES2574311T3 (es) * 2010-09-30 2016-06-16 Société Nationale d'Exploitation Industrielle des Tabacs et Allumettes S.E.I.T.A. Tabaco con contenido de cadmio reducido
WO2013119541A1 (en) * 2012-02-08 2013-08-15 Reynolds Technologies, Inc. Tobacco having altered amounts of environmental contaminants and methods for producing such lines

Also Published As

Publication number Publication date
US20190387702A1 (en) 2019-12-26
PH12018501304A1 (en) 2019-02-11
CA3010142A1 (en) 2017-08-03
JP7129335B2 (ja) 2022-09-01
US11213004B2 (en) 2022-01-04
KR20180107123A (ko) 2018-10-01
WO2017129739A1 (en) 2017-08-03
MX2018009002A (es) 2018-11-19
AR107478A1 (es) 2018-05-02
AU2017211970A1 (en) 2018-07-12
EP3407704A1 (en) 2018-12-05
JP2019503187A (ja) 2019-02-07
CN108697059B (zh) 2023-06-16
CO2018007797A2 (es) 2018-08-10
EP3407704B1 (en) 2024-08-14
CN108697059A (zh) 2018-10-23
EA201891707A1 (ru) 2018-12-28

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B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]