BR112018002914A2 - plants with reduced asparagine content - Google Patents

plants with reduced asparagine content

Info

Publication number
BR112018002914A2
BR112018002914A2 BR112018002914A BR112018002914A BR112018002914A2 BR 112018002914 A2 BR112018002914 A2 BR 112018002914A2 BR 112018002914 A BR112018002914 A BR 112018002914A BR 112018002914 A BR112018002914 A BR 112018002914A BR 112018002914 A2 BR112018002914 A2 BR 112018002914A2
Authority
BR
Brazil
Prior art keywords
sequence
seq
plants
asparagine synthetase
reduced
Prior art date
Application number
BR112018002914A
Other languages
Portuguese (pt)
Inventor
Bovet Lucien
Original Assignee
Philip Morris Products Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Philip Morris Products Sa filed Critical Philip Morris Products Sa
Publication of BR112018002914A2 publication Critical patent/BR112018002914A2/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/12—Leaves
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/18—Treatment of tobacco products or tobacco substitutes
    • A24B15/20—Biochemical treatment
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B3/00—Preparing tobacco in the factory
    • A24B3/12—Steaming, curing, or flavouring tobacco
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
    • C12Y603/05—Carbon-nitrogen ligases with glutamine as amido-N-donor (6.3.5)
    • C12Y603/05004—Asparagine synthase (glutamine-hydrolyzing) (6.3.5.4)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Botany (AREA)
  • Physiology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Manufacture Of Tobacco Products (AREA)
  • Peptides Or Proteins (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Fertilizers (AREA)

Abstract

é descrita neste documento uma planta mutante, de ocorrência não natural ou transgênica ou parte da mesma com a expressão ou atividade reduzida da asparagina sintetase, a referida asparagina sintetase compreendendo, consistindo em ou consistindo essencialmente em: (i) uma sequência de polinucleotídeo compreendendo, consistindo em ou consistindo essencialmente em uma sequência que tem pelo menos 90% da identidade de sequência para seq id no: 1 ou pelo menos 72% da identidade de sequência para seq id no: 3 ou seq id no: 5 ou seq id no: 7; ou (ii) um polipeptídeo codificado pelo polinucleotídeo apresentado em (i); ou (iii) um polipeptídeo que tem, pelo menos, 78% de identidade de sequência para seq id no: 2 ou seq id no: 4 ou seq id no: 6 ou seq id no: 8; em que a expressão ou atividade da asparagina sintetase apresentada em (i), (ii) ou (iii) é reduzida em comparação a uma planta de controle.A mutant, non-naturally occurring or transgenic plant or part thereof with reduced expression or activity of asparagine synthetase is described herein, said asparagine synthetase comprising, consisting of or consisting essentially of: (i) a polynucleotide sequence comprising, consisting of or consisting essentially of a sequence that has at least 90% sequence identity for seq id no: 1 or at least 72% sequence identity for seq id no: 3 or seq id no: 5 or seq id no: 7; or (ii) a polypeptide encoded by the polynucleotide set forth in (i); or (iii) a polypeptide having at least 78% sequence identity for sequence id: 2 or sequence id: 4 or sequence id: 6 or sequence id: 8; wherein the expression or activity of asparagine synthetase given in (i), (ii) or (iii) is reduced compared to a control plant.

BR112018002914A 2015-09-09 2016-09-06 plants with reduced asparagine content BR112018002914A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP15184528 2015-09-09
PCT/EP2016/070972 WO2017042162A1 (en) 2015-09-09 2016-09-06 Plants with reduced asparagine content

Publications (1)

Publication Number Publication Date
BR112018002914A2 true BR112018002914A2 (en) 2018-11-06

Family

ID=54105683

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112018002914A BR112018002914A2 (en) 2015-09-09 2016-09-06 plants with reduced asparagine content

Country Status (19)

Country Link
US (1) US10883114B2 (en)
EP (1) EP3346826A1 (en)
JP (1) JP6810134B2 (en)
KR (1) KR102207550B1 (en)
CN (1) CN107920489A (en)
AR (1) AR105968A1 (en)
AU (1) AU2016318423B2 (en)
BR (1) BR112018002914A2 (en)
CA (1) CA2998286C (en)
HK (1) HK1255341A1 (en)
IL (1) IL257305B (en)
MX (1) MX2018002695A (en)
MY (1) MY187046A (en)
NZ (1) NZ738897A (en)
PH (1) PH12017502292A1 (en)
RU (1) RU2742725C2 (en)
UA (1) UA125478C2 (en)
WO (1) WO2017042162A1 (en)
ZA (1) ZA201708411B (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019185699A1 (en) 2018-03-28 2019-10-03 Philip Morris Products S.A. Modulating reducing sugar content in a plant
AR115022A1 (en) 2018-03-28 2020-11-18 Philip Morris Products Sa MODULATION OF AMINO ACID CONTENT IN A PLANT
US11976286B2 (en) 2018-12-30 2024-05-07 Philip Morris Products S.A. Modulation of nitrate levels in plants via mutation of nitrate reductase
EP4041896A1 (en) 2019-10-01 2022-08-17 Philip Morris Products S.A. Modulating reducing sugar content in a plant (inv)
EP4041753A1 (en) 2019-10-01 2022-08-17 Philip Morris Products S.A. Modulating sugar and amino acid content in a plant (sultr3)
CN112931182B (en) * 2021-01-29 2022-06-07 四川农业大学 Method for improving recombination frequency of wheat chromosome
WO2023036691A1 (en) 2021-09-10 2023-03-16 Philip Morris Products S.A. Modulating alkaloid profiles in nicotiana tabacum
WO2023117661A1 (en) 2021-12-20 2023-06-29 Philip Morris Products S.A. Increasing anatabine in tobacco leaf by regulating methyl putrescine oxidase
WO2023117701A1 (en) 2021-12-21 2023-06-29 Philip Morris Products S.A. Modulation of nicotine production by alteration of nicotinamidase expression or function in plants
CN114766359B (en) * 2022-04-22 2023-03-31 华中农业大学 Test-tube plantlet support and application thereof in citrus stem tip micro-bud grafting detoxification
WO2024079137A1 (en) 2022-10-13 2024-04-18 Philip Morris Products S.A. Increasing leaf biomass and nitrogen use efficiency by regulating ntp2
CN116574719A (en) * 2023-06-28 2023-08-11 四川农业大学 AsMIPS1 gene cloning, expression vector construction and application

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ567293A (en) * 2005-09-20 2011-12-22 Simplot Co J R Low acrylamide foods obtained from plants with reduced asparagine levels
AU2013200664B2 (en) * 2005-09-20 2015-04-30 J.R. Simplot Company Low acrylamide foods
RU2324737C1 (en) * 2006-10-18 2008-05-20 Институт цитологии и генетики Сибирского отделения Российской академии наук (СО РАН) Process of obtaining transgene tobacco plants with increaed content of prolyl
JP4953808B2 (en) 2006-12-29 2012-06-13 フルタ電機株式会社 Drying method controlled by control panel of leaf tobacco monitoring control panel
AR074925A1 (en) * 2008-12-29 2011-02-23 Evogene Ltd POLINUCLEOTIDES, CODED POLYPEPTIDES, AND METHODS TO USE THEM TO MODULATE TOLERANCE TO ABIOTIC STRESS, BIOMASS AND / OR PERFORMANCE IN PLANTS THAT EXPRESS THEM
KR101214690B1 (en) * 2010-10-15 2012-12-21 고려대학교 산학협력단 Pepper (Capsicum annuum) asparagine synthetase 1 (CaAS1) gene and screening method of plant disease resistance using the same
CN102121018A (en) * 2010-12-22 2011-07-13 上海大学 Gene with asparagine synthetase function, encoding protein and application thereof
US8716571B2 (en) * 2011-09-21 2014-05-06 Reynolds Technologies, Inc. Tobacco having reduced amounts of amino acids and methods for producing such lines
EP2757872B1 (en) * 2011-09-21 2018-11-21 Reynolds Technologies, Inc. Tobacco having reduced amounts of amino acids and methods for producing such lines
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WO2014144094A1 (en) 2013-03-15 2014-09-18 J.R. Simplot Company Tal-mediated transfer dna insertion

Also Published As

Publication number Publication date
UA125478C2 (en) 2022-03-30
KR20180051530A (en) 2018-05-16
RU2742725C2 (en) 2021-02-10
AU2016318423A1 (en) 2018-04-05
HK1255341A1 (en) 2019-08-16
US20190169628A1 (en) 2019-06-06
CA2998286A1 (en) 2017-03-16
JP2018527934A (en) 2018-09-27
US10883114B2 (en) 2021-01-05
CA2998286C (en) 2023-11-07
EP3346826A1 (en) 2018-07-18
PH12017502292A1 (en) 2018-06-25
MX2018002695A (en) 2018-04-13
MY187046A (en) 2021-08-27
WO2017042162A1 (en) 2017-03-16
IL257305A (en) 2018-03-29
KR102207550B1 (en) 2021-01-27
AR105968A1 (en) 2017-11-29
AU2016318423B2 (en) 2022-01-20
RU2018112407A (en) 2019-10-09
JP6810134B2 (en) 2021-01-06
CN107920489A (en) 2018-04-17
ZA201708411B (en) 2019-01-30
IL257305B (en) 2022-06-01
RU2018112407A3 (en) 2020-02-25
NZ738897A (en) 2019-10-25

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Legal Events

Date Code Title Description
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]

Free format text: MANTIDO O INDEFERIMENTO UMA VEZ QUE NAO FOI APRESENTADO RECURSO DENTRO DO PRAZO LEGAL.MANTIDO O INDEFERIMENTO UMA VEZ QUE NAO FOI APRESENTADO RECURSO DENTRO DO PRAZO LEGAL.