RU2721799C2 - Уменьшение содержания табак-специфичных нитрозаминов посредством изменения пути ассимиляции нитратов - Google Patents

Уменьшение содержания табак-специфичных нитрозаминов посредством изменения пути ассимиляции нитратов Download PDF

Info

Publication number
RU2721799C2
RU2721799C2 RU2017114356A RU2017114356A RU2721799C2 RU 2721799 C2 RU2721799 C2 RU 2721799C2 RU 2017114356 A RU2017114356 A RU 2017114356A RU 2017114356 A RU2017114356 A RU 2017114356A RU 2721799 C2 RU2721799 C2 RU 2721799C2
Authority
RU
Russia
Prior art keywords
tobacco
plant
nitrate reductase
plants
modified
Prior art date
Application number
RU2017114356A
Other languages
English (en)
Russian (ru)
Other versions
RU2017114356A (ru
RU2017114356A3 (enExample
Inventor
Цзяньли ЛУ
Рэмси С. ЛЬЮИС
Ральф ДЬЮИ
Люсьен БОВЕ
Original Assignee
Филип Моррис Продактс С.А.
Норт Каролина Стейт Юниверсити
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Филип Моррис Продактс С.А., Норт Каролина Стейт Юниверсити filed Critical Филип Моррис Продактс С.А.
Publication of RU2017114356A publication Critical patent/RU2017114356A/ru
Publication of RU2017114356A3 publication Critical patent/RU2017114356A3/ru
Application granted granted Critical
Publication of RU2721799C2 publication Critical patent/RU2721799C2/ru

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/18—Treatment of tobacco products or tobacco substitutes
    • A24B15/24—Treatment of tobacco products or tobacco substitutes by extraction; Tobacco extracts
    • A24B15/241—Extraction of specific substances
    • A24B15/245—Nitrosamines
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/18—Treatment of tobacco products or tobacco substitutes
    • A24B15/20—Biochemical treatment
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/18—Treatment of tobacco products or tobacco substitutes
    • A24B15/24—Treatment of tobacco products or tobacco substitutes by extraction; Tobacco extracts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0044—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on other nitrogen compounds as donors (1.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y107/00—Oxidoreductases acting on other nitrogenous compounds as donors (1.7)
    • C12Y107/01—Oxidoreductases acting on other nitrogenous compounds as donors (1.7) with NAD+ or NADP+ as acceptor (1.7.1)
    • C12Y107/01001—Nitrate reductase (NADH) (1.7.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y107/00—Oxidoreductases acting on other nitrogenous compounds as donors (1.7)
    • C12Y107/01—Oxidoreductases acting on other nitrogenous compounds as donors (1.7) with NAD+ or NADP+ as acceptor (1.7.1)
    • C12Y107/01002—Nitrate reductase [NAD(P)H] (1.7.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y107/00—Oxidoreductases acting on other nitrogenous compounds as donors (1.7)
    • C12Y107/01—Oxidoreductases acting on other nitrogenous compounds as donors (1.7) with NAD+ or NADP+ as acceptor (1.7.1)
    • C12Y107/01003—Nitrate reductase (NADPH) (1.7.1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y107/00—Oxidoreductases acting on other nitrogenous compounds as donors (1.7)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Manufacture Of Tobacco Products (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Physiology (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
RU2017114356A 2014-09-26 2015-09-23 Уменьшение содержания табак-специфичных нитрозаминов посредством изменения пути ассимиляции нитратов RU2721799C2 (ru)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP14186705 2014-09-26
EP14186705.1 2014-09-26
EP14194798.6 2014-11-25
EP14194798 2014-11-25
PCT/EP2015/071919 WO2016046288A1 (en) 2014-09-26 2015-09-23 Reducing tobacco specific nitrosamines through alteration of the nitrate assimilation pathway

Publications (3)

Publication Number Publication Date
RU2017114356A RU2017114356A (ru) 2018-10-29
RU2017114356A3 RU2017114356A3 (enExample) 2019-04-29
RU2721799C2 true RU2721799C2 (ru) 2020-05-22

Family

ID=54148547

Family Applications (1)

Application Number Title Priority Date Filing Date
RU2017114356A RU2721799C2 (ru) 2014-09-26 2015-09-23 Уменьшение содержания табак-специфичных нитрозаминов посредством изменения пути ассимиляции нитратов

Country Status (12)

Country Link
US (1) US11666082B2 (enExample)
EP (1) EP3198015A1 (enExample)
JP (1) JP6921740B2 (enExample)
KR (1) KR102524798B1 (enExample)
CN (1) CN106998787A (enExample)
BR (1) BR112017003842B1 (enExample)
CA (1) CA2959245A1 (enExample)
CO (1) CO2017004076A2 (enExample)
MX (1) MX2017003932A (enExample)
PH (1) PH12017500313A1 (enExample)
RU (1) RU2721799C2 (enExample)
WO (1) WO2016046288A1 (enExample)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8466187B2 (en) 2007-09-18 2013-06-18 Thermolife International, Llc Amino acid compositions
CN107250148B (zh) 2014-12-03 2021-04-16 安捷伦科技有限公司 具有化学修饰的指导rna
ES2884838T3 (es) 2015-04-06 2021-12-13 Univ Leland Stanford Junior ARN guía químicamente modificados para la regulación génica mediada por CRISPR/CAS
BR112017023719A2 (pt) * 2015-05-05 2018-07-17 Univ North Carolina State planta de tabaco, parte de planta e/ou célula de planta, semente, cultura de planta, produto de tabaco, método para reduzir pon e/ou nnk, método de produção de uma planta e método para produzir um produto de tabaco
US10767175B2 (en) 2016-06-08 2020-09-08 Agilent Technologies, Inc. High specificity genome editing using chemically modified guide RNAs
CN106036984B (zh) * 2016-07-03 2017-08-08 赖高生 一种固定化蜗牛酶制备烟草浸膏的方法
CN111801423A (zh) 2018-03-28 2020-10-20 菲利普莫里斯生产公司 调节植物中的氨基酸含量
BR112020017430A2 (pt) 2018-03-28 2021-01-19 Philip Morris Products S.A. Modulação do teor de açúcar redutor em uma planta
CN108424922B (zh) * 2018-04-20 2021-04-16 云南省烟草农业科学研究院 一种降低尼古丁转化率的cyp82e10基因错义突变体m271及其应用
GB201812603D0 (en) * 2018-08-02 2018-09-19 British American Tobacco Investments Ltd Method
DE102018216970A1 (de) * 2018-10-04 2020-04-09 Robert Bosch Gmbh Brennstoffeinspritzvorrichtung
CN113271768B (zh) * 2018-12-30 2024-02-06 菲利普莫里斯生产公司 经由硝酸盐还原酶的突变来调节植物中的硝酸盐水平
WO2022192743A1 (en) * 2021-03-11 2022-09-15 Thermolife International, Llc Enhanced nitrate, elemental metal, and amino acid compositions and methods of use
BR112022005552A2 (pt) 2019-10-01 2022-06-28 Philip Morris Products Sa Modulação da redução do teor de açúcar em uma planta (inv)
US12419264B2 (en) 2019-10-01 2025-09-23 Philip Morris Products S.A. Modulating sugar and amino acid content in a plant (SULTR3)
CN110747208B (zh) * 2019-11-28 2022-04-22 海南大学 一种木薯硝酸还原酶基因及其过量表达载体的构建和抗病应用
CN110819640B (zh) * 2019-12-19 2022-06-21 中国烟草总公司郑州烟草研究院 烟草NtNRP1基因其应用
CN115516098A (zh) * 2020-01-17 2022-12-23 奥驰亚客户服务有限公司 与改进的氮利用效率有关的方法和组合物
WO2022104157A1 (en) 2020-11-12 2022-05-19 Thermolife International, Llc Methods of increasing blood oxygen saturation
US11865139B2 (en) 2020-11-12 2024-01-09 Thermolife International, Llc Method of treating migraines and headaches
CN116583176B (zh) * 2020-12-09 2024-12-31 日本烟草产业株式会社 烟草植物及烟草制品
IL315206A (en) 2021-02-11 2024-10-01 Thermolife Int Llc A method of administering nitric oxide gas
US11884915B2 (en) 2021-09-10 2024-01-30 Agilent Technologies, Inc. Guide RNAs with chemical modification for prime editing
WO2023117661A1 (en) 2021-12-20 2023-06-29 Philip Morris Products S.A. Increasing anatabine in tobacco leaf by regulating methyl putrescine oxidase
WO2023117701A1 (en) 2021-12-21 2023-06-29 Philip Morris Products S.A. Modulation of nicotine production by alteration of nicotinamidase expression or function in plants
US20240132900A1 (en) * 2022-02-24 2024-04-25 University Of Florida Research Foundation, Incorporated T-DNA Free Gene Editing through Transient Suppressing POLQ in Plants
JP2025533986A (ja) 2022-10-13 2025-10-09 フィリップ・モーリス・プロダクツ・ソシエテ・アノニム Ｎｔｐ２を調節することによって葉バイオマスおよび窒素利用効率を増加させる
KR20250139371A (ko) 2023-02-02 2025-09-23 필립모리스 프로덕츠 에스.에이. 당 수송체의 조절
CN120476136A (zh) 2023-02-02 2025-08-12 菲利普莫里斯生产公司 编码赖氨酸酮戊二酸还原酶的基因的调节
WO2025003105A1 (en) 2023-06-29 2025-01-02 Philip Morris Products S.A. Modulation of genes coding for glutamate dehydrogenase

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2324737C1 (ru) * 2006-10-18 2008-05-20 Институт цитологии и генетики Сибирского отделения Российской академии наук (СО РАН) Способ получения трансгенных растений табака с повышенным содержанием пролина
WO2013140155A1 (en) * 2012-03-20 2013-09-26 British Americal Tobacco (Investments) Limited Transgenic plants with altered nitrate levels in leaves

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US5990387A (en) 1988-06-10 1999-11-23 Pioneer Hi-Bred International, Inc. Stable transformation of plant cells
US5879918A (en) 1989-05-12 1999-03-09 Pioneer Hi-Bred International, Inc. Pretreatment of microprojectiles prior to using in a particle gun
US5932782A (en) 1990-11-14 1999-08-03 Pioneer Hi-Bred International, Inc. Plant transformation method using agrobacterium species adhered to microprojectiles
EP0652965A1 (en) 1992-07-27 1995-05-17 Pioneer Hi-Bred International, Inc. An improved method of agrobacterium-mediated transformation of cultured soybean cells
US5981840A (en) 1997-01-24 1999-11-09 Pioneer Hi-Bred International, Inc. Methods for agrobacterium-mediated transformation
KR20030029885A (ko) * 2000-08-30 2003-04-16 노쓰 캐롤라이나 스테이트 유니버시티 단백질 함량을 변화시키는 분자 미끼를 포함하는유전자이식 식물
WO2002100199A2 (en) * 2001-06-08 2002-12-19 Vector Tobacco Ltd. Modifying nicotine and nitrosamine levels in tobacco
KR20060128821A (ko) * 2003-08-19 2006-12-14 22엔디 센츄리 리미티드, 엘엘씨 감소된 노출 담배 제품
RU2257711C2 (ru) * 2003-10-06 2005-08-10 Всероссийский научно-исследовательский институт защиты растений Композиция на основе хитозана, снижающая содержание нитратов в растениеводческой продукции
EP2330191A1 (en) * 2003-10-16 2011-06-08 U.S. Smokeless Tobacco Company Cloning of cytochrome P450 genes from nicotiana
US20070199097A1 (en) * 2004-09-03 2007-08-23 U.S. Smokeless Tobacco Company Tobacco plants having a mutation in a nicotine demethylase gene
EP2087123B1 (en) 2006-10-13 2011-08-10 North Carolina State University Alteration of tobacco alkaloid content through modification of specific cytochrome p450 genes
BRPI0807783A2 (pt) * 2007-02-23 2014-06-24 Us Smokeless Tobacco Co Composição de tabaco sem fumaça, métodos para produzir uma variedade de planta e para preparar tabaco tendo amargor reduzido, planta de tabaco, variedade de planta, e, tabaco curado.
CA2681193C (en) * 2007-03-16 2016-01-26 New York University Pericycle-specific expression of microrna167 in transgenic plants
BRPI0820042B1 (pt) * 2007-11-12 2020-05-19 North Carolina State University método de obtenção de uma planta de tabaco, ou célula ou parte desta, tendo níveis reduzidos de nornicotina, produto de tabaco, método para fabricar um produto de tabaco, polinucleotídeo isolado, cassete de expressão, polipetpídeo isolado e método de obtenção de uma planta, ou parte de planta desta, do gênero nicotiana
PH12012501393B1 (en) 2010-01-15 2017-10-25 Univ North Carolina State Compositions and methods for minimizing nornicotine synthesis in tobacco
EP2537929B1 (en) * 2010-02-17 2017-04-12 Japan Tobacco, Inc. Plant component regulation factor, and use thereof
AP2013006825A0 (en) * 2010-09-22 2013-04-30 British American Tabacco Investments Ltd Transgenic plants
BR112013021515A2 (pt) 2011-02-28 2018-06-12 Univ North Carolina State plantas congênitas de tabaco ncbex1f, ncbex1ms e nc ex90
ITMI20121419A1 (it) * 2012-08-08 2014-02-09 Fattoria Autonoma Tabacchi S C A R L Metodo per la produzione di tabacco volto a ridurre il tenore di nitrosammine.

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2324737C1 (ru) * 2006-10-18 2008-05-20 Институт цитологии и генетики Сибирского отделения Российской академии наук (СО РАН) Способ получения трансгенных растений табака с повышенным содержанием пролина
WO2013140155A1 (en) * 2012-03-20 2013-09-26 British Americal Tobacco (Investments) Limited Transgenic plants with altered nitrate levels in leaves

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
GETU BEYENE et al., Two new cysteine proteinases with specific expression patterns in mature and senescent tobacco (Nicotiana tabacum L.) leaves, Journal of Experimental Botany, 2006, Vol. 57, No. 6, pp. 1431-1443. *
LAURENT NUSSAUME et al., Post-Transcriptional Regulation of Nitrate Reductase by Light Is Abolished by an N-Terminal Deletion, The Plant Cell, 1995, Vol. 7, pp.611-621 *
UNNI S. LEA et al., Posttranslational Regulation of Nitrate Reductase Strongly Affects the Levels of Free Amino Acids and Nitrate, whereas Transcriptional Regulation Has Only Minor Influence, Plant Physiol, 2006, Vol. 140, pp.1085-1094. *
UNNI S. LEA et al., Posttranslational Regulation of Nitrate Reductase Strongly Affects the Levels of Free Amino Acids and Nitrate, whereas Transcriptional Regulation Has Only Minor Influence, Plant Physiol, 2006, Vol. 140, pp.1085-1094. LAURENT NUSSAUME et al., Post-Transcriptional Regulation of Nitrate Reductase by Light Is Abolished by an N-Terminal Deletion, The Plant Cell, 1995, Vol. 7, pp.611-621. *

Also Published As

Publication number Publication date
US20170303582A1 (en) 2017-10-26
KR102524798B1 (ko) 2023-04-24
PH12017500313A1 (en) 2017-07-17
CO2017004076A2 (es) 2017-09-20
RU2017114356A (ru) 2018-10-29
JP6921740B2 (ja) 2021-08-18
RU2017114356A3 (enExample) 2019-04-29
KR20170078599A (ko) 2017-07-07
JP2017529850A (ja) 2017-10-12
BR112017003842B1 (pt) 2024-02-20
CA2959245A1 (en) 2016-03-31
US11666082B2 (en) 2023-06-06
CN106998787A (zh) 2017-08-01
WO2016046288A1 (en) 2016-03-31
BR112017003842A2 (pt) 2017-12-19
EP3198015A1 (en) 2017-08-02
MX2017003932A (es) 2017-06-30

Similar Documents

Publication Publication Date Title
RU2721799C2 (ru) Уменьшение содержания табак-специфичных нитрозаминов посредством изменения пути ассимиляции нитратов
JP7280245B2 (ja) タバコにおける改善された窒素利用効率に関する方法および組成物
KR20170002444A (ko) 식물 내 니코틴의 노르니코틴으로의 변환 감소
US20240218386A1 (en) Compositions and Methods for Producing Tobacco Plants and Products Having Altered Alkaloid Levels
CN108271341A (zh) 用于生产烟草植物的组合物和方法以及具有改变的生物碱含量的制品
JP6956084B2 (ja) タバコベルベリンブリッジ酵素様の核酸の標的化された突然変異誘発
US20240376488A1 (en) Tobacco plants comprising reduced nicotine and reduced tobacco specific nitrosamines
KR20140057642A (ko) 니코티아나 타바쿰으로부터의 트레오닌 합성효소 및 그의 방법 및 용도
US20250243501A1 (en) Compositions and methods for producing tobacco plants and products having altered alkaloid levels with desirable leaf quality via manipulating leaf quality genes
EP4161255A1 (en) Compositions and methods for producing tobacco plants and products having altered alkaloid levels
US20190309317A1 (en) Compositions and methods for producing tobacco plants and products having altered alkaloid levels
WO2023230433A1 (en) Methods and compositions for regulating alkaloids in tobacco field
WO2024031038A1 (en) Methods and compositions for regulating alkaloids in tobacco
US20210230627A1 (en) Methods and compositions related to improved nitrogen use efficiency