CO2017004076A2 - Reducción de nitrosaminas específicas del tabaco mediante la alteración de la vía de asimilación de nitrato - Google Patents
Reducción de nitrosaminas específicas del tabaco mediante la alteración de la vía de asimilación de nitratoInfo
- Publication number
- CO2017004076A2 CO2017004076A2 CONC2017/0004076A CO2017004076A CO2017004076A2 CO 2017004076 A2 CO2017004076 A2 CO 2017004076A2 CO 2017004076 A CO2017004076 A CO 2017004076A CO 2017004076 A2 CO2017004076 A2 CO 2017004076A2
- Authority
- CO
- Colombia
- Prior art keywords
- tobacco
- altering
- reduction
- specific nitrosamines
- assimilation pathway
- Prior art date
Links
- 235000002637 Nicotiana tabacum Nutrition 0.000 title abstract 4
- 229910002651 NO3 Inorganic materials 0.000 title abstract 3
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 title abstract 3
- 241000208125 Nicotiana Species 0.000 title abstract 2
- 150000004005 nitrosamines Chemical class 0.000 title abstract 2
- 230000037361 pathway Effects 0.000 title abstract 2
- 244000061176 Nicotiana tabacum Species 0.000 abstract 2
- 235000019505 tobacco product Nutrition 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 1
Classifications
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/24—Treatment of tobacco products or tobacco substitutes by extraction; Tobacco extracts
- A24B15/241—Extraction of specific substances
- A24B15/245—Nitrosamines
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/20—Biochemical treatment
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/24—Treatment of tobacco products or tobacco substitutes by extraction; Tobacco extracts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0044—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on other nitrogen compounds as donors (1.7)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y107/00—Oxidoreductases acting on other nitrogenous compounds as donors (1.7)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y107/00—Oxidoreductases acting on other nitrogenous compounds as donors (1.7)
- C12Y107/01—Oxidoreductases acting on other nitrogenous compounds as donors (1.7) with NAD+ or NADP+ as acceptor (1.7.1)
- C12Y107/01001—Nitrate reductase (NADH) (1.7.1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y107/00—Oxidoreductases acting on other nitrogenous compounds as donors (1.7)
- C12Y107/01—Oxidoreductases acting on other nitrogenous compounds as donors (1.7) with NAD+ or NADP+ as acceptor (1.7.1)
- C12Y107/01002—Nitrate reductase [NAD(P)H] (1.7.1.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y107/00—Oxidoreductases acting on other nitrogenous compounds as donors (1.7)
- C12Y107/01—Oxidoreductases acting on other nitrogenous compounds as donors (1.7) with NAD+ or NADP+ as acceptor (1.7.1)
- C12Y107/01003—Nitrate reductase (NADPH) (1.7.1.3)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Nutrition Science (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Manufacture Of Tobacco Products (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Physiology (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP14186705 | 2014-09-26 | ||
| EP14194798 | 2014-11-25 | ||
| PCT/EP2015/071919 WO2016046288A1 (en) | 2014-09-26 | 2015-09-23 | Reducing tobacco specific nitrosamines through alteration of the nitrate assimilation pathway |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CO2017004076A2 true CO2017004076A2 (es) | 2017-09-20 |
Family
ID=54148547
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CONC2017/0004076A CO2017004076A2 (es) | 2014-09-26 | 2017-04-26 | Reducción de nitrosaminas específicas del tabaco mediante la alteración de la vía de asimilación de nitrato |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US11666082B2 (enExample) |
| EP (1) | EP3198015A1 (enExample) |
| JP (1) | JP6921740B2 (enExample) |
| KR (1) | KR102524798B1 (enExample) |
| CN (1) | CN106998787A (enExample) |
| BR (1) | BR112017003842B1 (enExample) |
| CA (1) | CA2959245A1 (enExample) |
| CO (1) | CO2017004076A2 (enExample) |
| MX (1) | MX2017003932A (enExample) |
| PH (1) | PH12017500313A1 (enExample) |
| RU (1) | RU2721799C2 (enExample) |
| WO (1) | WO2016046288A1 (enExample) |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US8466187B2 (en) | 2007-09-18 | 2013-06-18 | Thermolife International, Llc | Amino acid compositions |
| US12612370B2 (en) | 2011-03-02 | 2026-04-28 | Thermolife International, Llc | Amino acid compositions |
| CA2969619A1 (en) | 2014-12-03 | 2016-06-09 | Agilent Technologies, Inc. | Guide rna with chemical modifications |
| KR102888521B1 (ko) | 2015-04-06 | 2025-11-19 | 더 보드 어브 트러스티스 어브 더 리랜드 스탠포드 주니어 유니버시티 | Crispr/cas-매개 유전자 조절을 위한 화학적으로 변형된 가이드 rna |
| US20180291388A1 (en) * | 2015-05-05 | 2018-10-11 | North Carolina State University | Methods and compositions for reducing the tobacco specific nitrosamine nnk in tobacco |
| US10767175B2 (en) | 2016-06-08 | 2020-09-08 | Agilent Technologies, Inc. | High specificity genome editing using chemically modified guide RNAs |
| CN106036984B (zh) * | 2016-07-03 | 2017-08-08 | 赖高生 | 一种固定化蜗牛酶制备烟草浸膏的方法 |
| WO2019185699A1 (en) | 2018-03-28 | 2019-10-03 | Philip Morris Products S.A. | Modulating reducing sugar content in a plant |
| KR20200136921A (ko) | 2018-03-28 | 2020-12-08 | 필립모리스 프로덕츠 에스.에이. | 식물 내 아미노산 함량 조절 |
| CN108424922B (zh) * | 2018-04-20 | 2021-04-16 | 云南省烟草农业科学研究院 | 一种降低尼古丁转化率的cyp82e10基因错义突变体m271及其应用 |
| GB201812603D0 (en) | 2018-08-02 | 2018-09-19 | British American Tobacco Investments Ltd | Method |
| DE102018216970A1 (de) * | 2018-10-04 | 2020-04-09 | Robert Bosch Gmbh | Brennstoffeinspritzvorrichtung |
| WO2020141062A1 (en) | 2018-12-30 | 2020-07-09 | Philip Morris Products S.A. | Modulation of nitrate levels in plants via mutation of nitrate reductase |
| WO2022192743A1 (en) * | 2021-03-11 | 2022-09-15 | Thermolife International, Llc | Enhanced nitrate, elemental metal, and amino acid compositions and methods of use |
| JP7659547B2 (ja) | 2019-10-01 | 2025-04-09 | フィリップ・モーリス・プロダクツ・ソシエテ・アノニム | 植物における還元糖含有量の調節（ｉｎｖ） |
| CN114514321A (zh) | 2019-10-01 | 2022-05-17 | 菲利普莫里斯生产公司 | 调节植物中的糖和氨基酸含量（sultr3） |
| CN110747208B (zh) * | 2019-11-28 | 2022-04-22 | 海南大学 | 一种木薯硝酸还原酶基因及其过量表达载体的构建和抗病应用 |
| CN110819640B (zh) * | 2019-12-19 | 2022-06-21 | 中国烟草总公司郑州烟草研究院 | 烟草NtNRP1基因其应用 |
| WO2021146585A1 (en) * | 2020-01-17 | 2021-07-22 | Altria Client Services Llc | Methods and compositions related to improved nitrogen use efficiency |
| JP2024541338A (ja) | 2020-11-12 | 2024-11-08 | サーモライフ インターナショナル， エルエルシー | 血中酸素飽和度を増大させる方法 |
| US11865139B2 (en) | 2020-11-12 | 2024-01-09 | Thermolife International, Llc | Method of treating migraines and headaches |
| JP7696368B2 (ja) * | 2020-12-09 | 2025-06-20 | 日本たばこ産業株式会社 | タバコ植物及びたばこ製品 |
| AU2022220004A1 (en) | 2021-02-11 | 2023-08-17 | Thermolife International, Llc | A method of administering nitric oxide gas |
| JP2024534945A (ja) | 2021-09-10 | 2024-09-26 | アジレント・テクノロジーズ・インク | 化学修飾を有するプライム編集のためのガイドｒｎａ |
| US20250043298A1 (en) | 2021-12-20 | 2025-02-06 | Philip Morris Products S.A. | Increasing anatabine in tobacco leaf by regulating methyl putrescine oxidase |
| US20250084427A1 (en) | 2021-12-21 | 2025-03-13 | Philip Morris Products S.A. | Modulation of nicotine production by alteration of nicotinamidase expression or function in plants |
| US20240132900A1 (en) * | 2022-02-24 | 2024-04-25 | University Of Florida Research Foundation, Incorporated | T-DNA Free Gene Editing through Transient Suppressing POLQ in Plants |
| EP4602060A1 (en) | 2022-10-13 | 2025-08-20 | Philip Morris Products S.A. | Increasing leaf biomass and nitrogen use efficiency by regulating ntp2 |
| CN120530195A (zh) | 2023-02-02 | 2025-08-22 | 菲利普莫里斯生产公司 | 糖转运蛋白的调节 |
| KR20250139370A (ko) | 2023-02-02 | 2025-09-23 | 필립모리스 프로덕츠 에스.에이. | 리신 케토글루타레이트 환원효소 코딩 유전자 조절 |
| WO2025003105A1 (en) | 2023-06-29 | 2025-01-02 | Philip Morris Products S.A. | Modulation of genes coding for glutamate dehydrogenase |
| WO2026057635A1 (en) | 2024-09-12 | 2026-03-19 | Philip Morris Products S.A. | Modulation of nitrate levels in tobacco via mutation of nitrate reductase |
| WO2026082649A1 (en) | 2024-10-15 | 2026-04-23 | Philip Morris Products S.A. | Modulation of alkaloid levels in tobacco via mutation of quinolinate synthase |
Family Cites Families (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4945050A (en) | 1984-11-13 | 1990-07-31 | Cornell Research Foundation, Inc. | Method for transporting substances into living cells and tissues and apparatus therefor |
| US5990387A (en) | 1988-06-10 | 1999-11-23 | Pioneer Hi-Bred International, Inc. | Stable transformation of plant cells |
| US5879918A (en) | 1989-05-12 | 1999-03-09 | Pioneer Hi-Bred International, Inc. | Pretreatment of microprojectiles prior to using in a particle gun |
| US5932782A (en) | 1990-11-14 | 1999-08-03 | Pioneer Hi-Bred International, Inc. | Plant transformation method using agrobacterium species adhered to microprojectiles |
| JP2952041B2 (ja) | 1992-07-27 | 1999-09-20 | パイオニア ハイ−ブレッド インターナショナル，インコーポレイテッド | 培養ダイズ細胞のａｇｒｏｂａｃｔｅｒｉｕｍ媒介形質転換の改良法 |
| US5981840A (en) | 1997-01-24 | 1999-11-09 | Pioneer Hi-Bred International, Inc. | Methods for agrobacterium-mediated transformation |
| EP1724355A3 (en) * | 2000-08-30 | 2007-05-23 | North Carolina State University | Transgenic plants containing molecular decoys that alter protein content therein |
| CA2449920A1 (en) * | 2001-06-08 | 2002-12-19 | Vector Tobacco Ltd. | Modifying nicotine and nitrosamine levels in tobacco |
| AU2004266694A1 (en) * | 2003-08-19 | 2005-03-03 | 22Nd Century Limited, Llc | Reduced-exposure tobacco products |
| RU2257711C2 (ru) * | 2003-10-06 | 2005-08-10 | Всероссийский научно-исследовательский институт защиты растений | Композиция на основе хитозана, снижающая содержание нитратов в растениеводческой продукции |
| CN1926235B (zh) * | 2003-10-16 | 2012-07-18 | 美国无烟烟草有限责任公司 | 从烟草克隆细胞色素p450基因 |
| US20070199097A1 (en) * | 2004-09-03 | 2007-08-23 | U.S. Smokeless Tobacco Company | Tobacco plants having a mutation in a nicotine demethylase gene |
| BRPI0717355B1 (pt) * | 2006-10-13 | 2018-01-16 | North Carolina State University | Método para obtenção de uma planta transgênica de nicotina, método para obtenção de uma semente; construto de ácido nucléico recombinante; método de redução da conversão de nicotina em nornicotina em uma planta de nicotina |
| RU2324737C1 (ru) | 2006-10-18 | 2008-05-20 | Институт цитологии и генетики Сибирского отделения Российской академии наук (СО РАН) | Способ получения трансгенных растений табака с повышенным содержанием пролина |
| CN101686731B (zh) * | 2007-02-23 | 2013-05-08 | 美国无烟烟草有限责任公司 | 新型烟草组合物及制备方法 |
| WO2008115487A1 (en) * | 2007-03-16 | 2008-09-25 | New York University | Methods of affecting plant growth with microrna |
| WO2009064771A2 (en) * | 2007-11-12 | 2009-05-22 | North Carolina State University | Alteration of tobacco alkaloid content through modification of specific cytochrome p450 genes |
| PH12012501393B1 (en) | 2010-01-15 | 2017-10-25 | Univ North Carolina State | Compositions and methods for minimizing nornicotine synthesis in tobacco |
| EP2537929B1 (en) * | 2010-02-17 | 2017-04-12 | Japan Tobacco, Inc. | Plant component regulation factor, and use thereof |
| US9322029B2 (en) * | 2010-09-22 | 2016-04-26 | British American Tobacco (Investments) Limited | Transgenic plants with reduced nitrate content |
| US9096864B2 (en) | 2011-02-28 | 2015-08-04 | North Carolina State University | Tobacco inbred plants NCBEX1F, NCBEX1MS, and NC EX90 |
| GB201204869D0 (en) | 2012-03-20 | 2012-05-02 | Cambridge Advanced Tech | Transgenic plants |
| ITMI20121419A1 (it) * | 2012-08-08 | 2014-02-09 | Fattoria Autonoma Tabacchi S C A R L | Metodo per la produzione di tabacco volto a ridurre il tenore di nitrosammine. |
-
2015
- 2015-09-23 EP EP15766550.6A patent/EP3198015A1/en active Pending
- 2015-09-23 CA CA2959245A patent/CA2959245A1/en not_active Abandoned
- 2015-09-23 MX MX2017003932A patent/MX2017003932A/es unknown
- 2015-09-23 WO PCT/EP2015/071919 patent/WO2016046288A1/en not_active Ceased
- 2015-09-23 JP JP2017516332A patent/JP6921740B2/ja active Active
- 2015-09-23 KR KR1020177008015A patent/KR102524798B1/ko active Active
- 2015-09-23 BR BR112017003842-0A patent/BR112017003842B1/pt active IP Right Grant
- 2015-09-23 US US15/513,229 patent/US11666082B2/en active Active
- 2015-09-23 CN CN201580049814.7A patent/CN106998787A/zh active Pending
- 2015-09-23 RU RU2017114356A patent/RU2721799C2/ru active
-
2017
- 2017-02-21 PH PH12017500313A patent/PH12017500313A1/en unknown
- 2017-04-26 CO CONC2017/0004076A patent/CO2017004076A2/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| US20170303582A1 (en) | 2017-10-26 |
| RU2017114356A3 (enExample) | 2019-04-29 |
| CA2959245A1 (en) | 2016-03-31 |
| KR20170078599A (ko) | 2017-07-07 |
| BR112017003842B1 (pt) | 2024-02-20 |
| US11666082B2 (en) | 2023-06-06 |
| PH12017500313A1 (en) | 2017-07-17 |
| WO2016046288A1 (en) | 2016-03-31 |
| JP6921740B2 (ja) | 2021-08-18 |
| CN106998787A (zh) | 2017-08-01 |
| JP2017529850A (ja) | 2017-10-12 |
| MX2017003932A (es) | 2017-06-30 |
| RU2017114356A (ru) | 2018-10-29 |
| BR112017003842A2 (pt) | 2017-12-19 |
| KR102524798B1 (ko) | 2023-04-24 |
| RU2721799C2 (ru) | 2020-05-22 |
| EP3198015A1 (en) | 2017-08-02 |
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