CN106973703A - A kind of walnut nursery stock grafting and breeding method - Google Patents
A kind of walnut nursery stock grafting and breeding method Download PDFInfo
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- CN106973703A CN106973703A CN201710075313.7A CN201710075313A CN106973703A CN 106973703 A CN106973703 A CN 106973703A CN 201710075313 A CN201710075313 A CN 201710075313A CN 106973703 A CN106973703 A CN 106973703A
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- 235000009496 Juglans regia Nutrition 0.000 title claims abstract description 69
- 235000020234 walnut Nutrition 0.000 title claims abstract description 69
- 238000009395 breeding Methods 0.000 title claims abstract description 24
- 240000007049 Juglans regia Species 0.000 title 1
- 241000758789 Juglans Species 0.000 claims abstract description 73
- 235000015097 nutrients Nutrition 0.000 claims abstract description 52
- 241000196324 Embryophyta Species 0.000 claims abstract description 26
- YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 claims abstract description 24
- 239000002994 raw material Substances 0.000 claims abstract description 17
- IXVMHGVQKLDRKH-YEJCTVDLSA-N (22s,23s)-epibrassinolide Chemical compound C1OC(=O)[C@H]2C[C@H](O)[C@H](O)C[C@]2(C)[C@H]2CC[C@]3(C)[C@@H]([C@H](C)[C@H](O)[C@@H](O)[C@H](C)C(C)C)CC[C@H]3[C@@H]21 IXVMHGVQKLDRKH-YEJCTVDLSA-N 0.000 claims abstract description 16
- IXVMHGVQKLDRKH-VRESXRICSA-N Brassinolide Natural products O=C1OC[C@@H]2[C@@H]3[C@@](C)([C@H]([C@@H]([C@@H](O)[C@H](O)[C@H](C(C)C)C)C)CC3)CC[C@@H]2[C@]2(C)[C@@H]1C[C@H](O)[C@H](O)C2 IXVMHGVQKLDRKH-VRESXRICSA-N 0.000 claims abstract description 16
- 239000005980 Gibberellic acid Substances 0.000 claims abstract description 16
- 239000005708 Sodium hypochlorite Substances 0.000 claims abstract description 16
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 claims abstract description 16
- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical compound C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 claims abstract description 16
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 claims abstract description 16
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 14
- 230000001939 inductive effect Effects 0.000 claims abstract description 12
- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000000843 powder Substances 0.000 claims abstract description 12
- 229960004889 salicylic acid Drugs 0.000 claims abstract description 12
- 239000003638 chemical reducing agent Substances 0.000 claims abstract description 11
- 238000007654 immersion Methods 0.000 claims abstract description 9
- RSWGJHLUYNHPMX-UHFFFAOYSA-N Abietic-Saeure Natural products C12CCC(C(C)C)=CC2=CCC2C1(C)CCCC2(C)C(O)=O RSWGJHLUYNHPMX-UHFFFAOYSA-N 0.000 claims abstract description 7
- KHPCPRHQVVSZAH-HUOMCSJISA-N Rosin Natural products O(C/C=C/c1ccccc1)[C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 KHPCPRHQVVSZAH-HUOMCSJISA-N 0.000 claims abstract description 7
- KHPCPRHQVVSZAH-UHFFFAOYSA-N trans-cinnamyl beta-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OCC=CC1=CC=CC=C1 KHPCPRHQVVSZAH-UHFFFAOYSA-N 0.000 claims abstract description 7
- 239000000243 solution Substances 0.000 claims description 53
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 17
- 238000012545 processing Methods 0.000 claims description 15
- RBJIGQRZLITQJG-UHFFFAOYSA-N 2-(2,3-dichlorophenoxy)acetic acid Chemical class OC(=O)COC1=CC=CC(Cl)=C1Cl RBJIGQRZLITQJG-UHFFFAOYSA-N 0.000 claims description 10
- ZNJFBWYDHIGLCU-HWKXXFMVSA-N jasmonic acid Chemical compound CC\C=C/C[C@@H]1[C@@H](CC(O)=O)CCC1=O ZNJFBWYDHIGLCU-HWKXXFMVSA-N 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 9
- 239000012528 membrane Substances 0.000 claims description 9
- 238000003756 stirring Methods 0.000 claims description 7
- 238000005520 cutting process Methods 0.000 claims description 6
- 241001582888 Lobus Species 0.000 claims description 5
- 239000012895 dilution Substances 0.000 claims description 5
- 238000010790 dilution Methods 0.000 claims description 5
- ZNJFBWYDHIGLCU-UHFFFAOYSA-N jasmonic acid Natural products CCC=CCC1C(CC(O)=O)CCC1=O ZNJFBWYDHIGLCU-UHFFFAOYSA-N 0.000 claims description 5
- 238000010030 laminating Methods 0.000 claims description 5
- 238000002203 pretreatment Methods 0.000 claims description 5
- LUBJCRLGQSPQNN-UHFFFAOYSA-N 1-Phenylurea Chemical compound NC(=O)NC1=CC=CC=C1 LUBJCRLGQSPQNN-UHFFFAOYSA-N 0.000 claims description 4
- LCPDWSOZIOUXRV-UHFFFAOYSA-N phenoxyacetic acid Chemical compound OC(=O)COC1=CC=CC=C1 LCPDWSOZIOUXRV-UHFFFAOYSA-N 0.000 claims 2
- 230000029663 wound healing Effects 0.000 abstract description 14
- 230000012010 growth Effects 0.000 abstract description 8
- 230000004083 survival effect Effects 0.000 abstract description 6
- 239000000203 mixture Substances 0.000 abstract description 3
- 230000001954 sterilising effect Effects 0.000 abstract description 3
- 238000009472 formulation Methods 0.000 abstract description 2
- XEYINGFRHZCVKK-UHFFFAOYSA-N 2,2-dichloro-2-phenoxyacetic acid Chemical class OC(=O)C(Cl)(Cl)OC1=CC=CC=C1 XEYINGFRHZCVKK-UHFFFAOYSA-N 0.000 abstract 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 abstract 1
- 239000000460 chlorine Substances 0.000 abstract 1
- 229910052801 chlorine Inorganic materials 0.000 abstract 1
- 238000002360 preparation method Methods 0.000 abstract 1
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 abstract 1
- JUJWROOIHBZHMG-UHFFFAOYSA-N pyridine Substances C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 abstract 1
- 238000004659 sterilization and disinfection Methods 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 17
- 239000000463 material Substances 0.000 description 15
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 12
- 201000010099 disease Diseases 0.000 description 11
- 230000032823 cell division Effects 0.000 description 7
- 235000013399 edible fruits Nutrition 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 6
- 206010052428 Wound Diseases 0.000 description 5
- 208000027418 Wounds and injury Diseases 0.000 description 5
- 230000015572 biosynthetic process Effects 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 230000001488 breeding effect Effects 0.000 description 4
- QEWYKACRFQMRMB-UHFFFAOYSA-N fluoroacetic acid Chemical compound OC(=O)CF QEWYKACRFQMRMB-UHFFFAOYSA-N 0.000 description 4
- 238000003786 synthesis reaction Methods 0.000 description 4
- 235000013757 Juglans Nutrition 0.000 description 3
- 230000000844 anti-bacterial effect Effects 0.000 description 3
- 239000003337 fertilizer Substances 0.000 description 3
- 230000035876 healing Effects 0.000 description 3
- 230000001976 improved effect Effects 0.000 description 3
- 230000004060 metabolic process Effects 0.000 description 3
- 238000009333 weeding Methods 0.000 description 3
- 239000002023 wood Substances 0.000 description 3
- RFFLAFLAYFXFSW-UHFFFAOYSA-N 1,2-dichlorobenzene Chemical class ClC1=CC=CC=C1Cl RFFLAFLAYFXFSW-UHFFFAOYSA-N 0.000 description 2
- 244000241257 Cucumis melo Species 0.000 description 2
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 2
- 206010020649 Hyperkeratosis Diseases 0.000 description 2
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 2
- 235000011613 Pinus brutia Nutrition 0.000 description 2
- 241000018646 Pinus brutia Species 0.000 description 2
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 2
- 230000002350 accommodative effect Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000001276 controlling effect Effects 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 230000007850 degeneration Effects 0.000 description 2
- 239000000645 desinfectant Substances 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 230000035515 penetration Effects 0.000 description 2
- SCVFZCLFOSHCOH-UHFFFAOYSA-M potassium acetate Chemical compound [K+].CC([O-])=O SCVFZCLFOSHCOH-UHFFFAOYSA-M 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000001902 propagating effect Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- HABAPWZXRLIZDL-UHFFFAOYSA-N 2-chloro-2-phenoxyacetic acid Chemical compound OC(=O)C(Cl)OC1=CC=CC=C1 HABAPWZXRLIZDL-UHFFFAOYSA-N 0.000 description 1
- 244000144730 Amygdalus persica Species 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- 241000207199 Citrus Species 0.000 description 1
- 235000011511 Diospyros Nutrition 0.000 description 1
- 244000236655 Diospyros kaki Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000726221 Gemma Species 0.000 description 1
- 235000010254 Jasminum officinale Nutrition 0.000 description 1
- 240000005385 Jasminum sambac Species 0.000 description 1
- 235000006040 Prunus persica var persica Nutrition 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 150000001805 chlorine compounds Chemical group 0.000 description 1
- 235000020971 citrus fruits Nutrition 0.000 description 1
- 230000000763 evoking effect Effects 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 206010020718 hyperplasia Diseases 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
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- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000008654 plant damage Effects 0.000 description 1
- 239000005648 plant growth regulator Substances 0.000 description 1
- 239000003375 plant hormone Substances 0.000 description 1
- 235000011056 potassium acetate Nutrition 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 230000002786 root growth Effects 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/30—Grafting
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to plant graft technical field of cultivation, and in particular to a kind of walnut nursery stock grafting and breeding method.The walnut nursery stock grafting and breeding method is according to following 35 parts of raw material alcohol, 23 parts of sodium hypochlorite, 35 parts of streptomysin, 35 parts of gibberellic acid, 46 parts of brassinosteroid, 2,335 parts of dichlorphenoxyacetic acids, N (pyridine radicals of 3 chlorine 4) 35 parts of N ' phenylureas, 23 parts of rosin, 46 parts of jasmonic, 46 parts of salicylic acid, the configuration of 35 parts of root-inducing powder and 23 parts of water-loss reducer progress nutrient solution, then carry out stock and scion preparation, sterilization, grafting, immersion etc. and breed step.Rationally, energy walnut nursery stock shortens wound healing time to the walnut nursery stock grafting and breeding method scientific formulation, promotes growth, improves graft survival rate.
Description
Technical field
The present invention relates to plant graft technical field of cultivation, and in particular to a kind of walnut nursery stock grafting and breeding method.
Background technology
It, come what is carried out, is an important plant propagation skill that plant graft, which is using the function after plant injury with callus,
Art.During grafting, make two forming layers for hindering face close and tighten together, as a result because of hyperplasia, be healing each other to dimension pipe
The entirety linked together is organized, here it is the principle of grafting.Plant graft does not produce seed bearing fruit tree (such as to some
Some kinds of persimmon, citrus) breeding meaning it is great, can keep the merit of scion variety, and can having using stock
Sharp characteristic, reaches precocity fruiting, enhancing winter resistance, drought resistance, the ability of disease and insect resistance, moreover it is possible to economic utilization propagating materials, increase
In nursery stock quantity, the breeding for being usually used in fruit tree, forest, flowers, it is also used in melon vegetables nursery.
People gradually instead of seed seedling-raising in seedling fostering by constantly testing, groping using graft seedling growth method
Method, the plant of grafting survival can keep the merit of scion variety, and can utilize the advantageous feature of stock, strengthen cold-resistant
Property, the purpose such as drought-resistant, disease and insect resistance, moreover it is possible to economic utilization propagating materials, increase nursery stock quantity are to meet large-scale production
Need.Graft seedling growth method is usually used in the breeding of fruit tree, forest, flowers, is also widely used for the nursery of melon vegetables.But transferring
In termination process, often occur various practical problems, dead seedling after not emerging or emerge after some graftings, some wound healings are slow, have
Yellow seedling of long potential difference etc. after healing.
The content of the invention
The goal of the invention of the present invention is：For above-mentioned problem there is provided a kind of walnut nursery stock grafting and breeding method,
Walnut nursery stock wound healing time can be shortened and promote growth, walnut nursery stock is kept humidity for a long time, significantly shorten Juglans
Wood breeds the time, the effective survival rate for improving walnut seedling grafting.
To achieve these goals, the technical solution adopted by the present invention is as follows：
A kind of walnut nursery stock grafting and breeding method, specifically includes following steps：
(1) selection of raw material and its parts by weight：3-5 parts of alcohol, 2-3 parts of sodium hypochlorite, 3-5 parts of streptomysin, gibberellic acid 3-5
Part, 4-6 parts of brassinosteroid, 3-5 parts of 2,3- dichlorphenoxyacetic acids, N- (chloro- 4 pyridine radicals of 3-)-N ' -3-5 parts of phenylurea, rosin
2-3 parts of 2-3 parts, 4-6 parts of jasmonic, 4-6 parts of salicylic acid, 3-5 parts of root-inducing powder and water-loss reducer；
(2) prepared by nutrient solution：According to parts by weight in step (1), streptomysin and water are pressed 1:4500-5500 ratios carry out dilute
Release, add gibberellic acid, alcohol and sodium hypochlorite, be uniformly mixed, be modulated into nutrient solution A；By brassinosteroid, 2,3- bis-
After chlorophenoxyacetic acid and N- (chloro- 4 pyridine radicals of 3-)-N '-phenylurea mixing, stir, obtain mixed liquor A, then by mixed liquor A
1 is pressed with water:200-250 ratios are diluted, and are eventually adding rosin, continue to stir, and nutrient solution B is made；By root-inducing powder with
Water presses 1:750-850 ratios are carried out after water-soluble dilution, are added jasmonic and salicylic acid, are uniformly mixed, obtain mixed liquor
B, then water-loss reducer and mixed liquid B are pressed 1:150-250 ratio hybrid modulations, are uniformly mixed, and nutrient solution C is made；
(3) prepared by stock：From the wild seedling of walnut as stock, stock is removed into growing point and lobus cardiacus, in two sons
Leaf intermediate vertical down-cutting breach, bevel is smooth；
(4) scion collection and processing：Collection walnut sprout to be grafted is as scion, the about 0.9- under scion cotyledon
1.1cm is in young stem both sides and is whittled into two-sided wedge shape, and bevel is smooth；
(5) sterilize：Stock and scion are soaked in nutrient solution A, soak time is 10-12min；
(6) scion grafting pre-treatment：The scion sterilized is immersed in nutrient solution B, soak time is 2-3h；
(7) grafting：The stock handled well and scion are subjected to grafting, i.e., scion folding wedge gently inserted in face of quasi- stock otch
Enter, it is ensured that scion and stock grafting mouthful alignment, make two incisions laminating close, then twined scion and stock binding site with sealed membrane
It is tight sturdy, entered with rainwater-proof；
(8) soak：The walnut nursery stock grafted is put into nutrient solution C and soaked, soak time is 10-12h；
(9) plant：The walnut nursery stock of immersion treatment is transplanted in warmhouse booth, then is transplanted to field planting between field, field planting
Period carries out conventional field management.
Preferably, in step (1), the volumetric concentration of the alcohol is 70%-75%.
Preferably, in step (3), a length of 7-10mm of the cutting breach.
Preferably, in step (4), two-sided wedge-shaped a length of 7-10mm matches with stock breach.
Preferably, in step (7), the sealed membrane is Parafilm sealed membranes.
Preferably, in step (9), canopy temperature is 23-26 DEG C, relative air humidity 75-85%.
Preferably, in step (9), the time planted in greenhouse is 3-4 week.
In summary, by adopting the above-described technical solution, the beneficial effects of the invention are as follows：
Streptomysin is microbial source bactericide, can effectively prevent and treat phytobacterial disease, and gibberellic acid is except bactericidal action, also
Cell division and the elongation of stem can be promoted, sodium hypochlorite is chloride, broad-spectrum, effect is rapid, to virus, bacterium, fungi and
Gemma has stronger killing ability, by the kind that streptomysin, gibberellic acid and sodium hypochlorite suppress or killed bacterium is different, kills
Bacterium effect is also different, if single use, bactericidal range is narrow, and effect is poor, and alcohol has stronger sterilizing power, penetration power and wet
Profit is acted on, and the air on walnut nursery stock can be excluded, inside the infiltration walnut nursery stock of other disinfectants；The present invention is by above thing
Matter is combined, and accelerates the penetration of disinfectant, is increased killing bacterium scope, is effectively facilitated to stock and scion sterilizing
Effect, and also promote cell division, evoked callus is formed, and can shorten walnut nursery stock wound healing time, prevents plant disease
Disease occurs.
Brassinosteroid is plant hormone, can promote cell division and elongation, 2,3- dichlorphenoxyacetic acids and N- (3- chloro- 4
Pyridine radicals)-N '-phenylurea is artificial synthesized plant growth regulator, it is the similar thing of auximone, cell elongation can be promoted
And division, these materials are different in the degree for promoting cell division and elongation, and effect is also different, single using any
The speed of kind material, cell division and elongation decreases, and wound healing time is slow, but combines, cell division
And the speed of elongation is significantly improved, along with adhesive rosin, the wound of fruit tree is closed, film is formed in wound, is closed all kinds of
Non-anaerobic bactreia, is allowed to inactivate, controlling disease, keeps wound moisture, has effectively facilitated walnut nursery stock wound healing time, reduced
Germ prevents plant to infecting for wound from healthy growth.
Root-inducing powder can promote large biological molecule by strengthening, regulating and controlling the content of plant endogenous hormones and the activity of important enzyme
Synthesis, adjust plant metabolism intensity, induction plant adventitious root or adventitious bud formation, adjust plant metabolism intensity, be improved
The effect of nursery, the surrival rate of afforestation and crop yield, quality and resistance；The synthesis of jasmine acid-induced protein, promotes breathing to make
With and tuber character, improve plant resistance and defence capability；Salicylic acid can promote the synthesis of related protein, resist cause of disease
Microorganism, improves plant disease-resistant ability；Because the walnut seedling vitality of firm grafting is not strong, it is difficult to adapt to living environment, pole holds
Easily occur not emerge, turn to be yellow or the phenomenon such as death, above material mixing is modulated into nutrient solution functional diversities, plant can be promoted
Metabolism, promotes Self substances synthesis and activity, promotes cell elongation and division, make walnut nursery stock Rapid Rooting, shorten Juglans
Wood is bred the time, also effectively increases plant resistant power, plant is significantly carried in many-sides such as disease-resistant, degeneration-resistant and defence
Height, strengthens walnut nursery stock accommodative ability of environment, reduces disease infringement, walnut plant percent is greatly facilitated.And water-loss reducer
Addition, and walnut seedling root Water-saving effect enhancing, prevent because water shortage and caused by walnut nursery stock grow decline.
Embodiment
The invention discloses a kind of walnut nursery stock grafting and breeding method is simple to operate, scientific formulation rationally, can shorten walnut
Nursery stock wound healing time and promotion growth, make walnut nursery stock keep humidity for a long time, significantly shorten the walnut seedling-wood breeding time,
The effective survival rate for improving walnut seedling grafting.
Technical scheme is clearly and completely described below by specific embodiment.
Embodiment 1
A kind of walnut nursery stock grafting and breeding method, specifically includes following steps：
(1) raw material selection and its parts by weight：Volumetric concentration for 70% 3 parts of alcohol, 2 parts of sodium hypochlorite, 3 parts of streptomysin,
3 parts of gibberellic acid, 4 parts of brassinosteroid, 3 parts of 2,3- dichlorphenoxyacetic acids, N- (chloro- 4 pyridine radicals of 3-) 3 parts of-N '-phenylurea, pine
2 parts of fragrant 2 parts, 4 parts of jasmonic, 4 parts of salicylic acid, 3 parts of root-inducing powder and water-loss reducer；
(2) prepared by nutrient solution：According to parts by weight in step (1), streptomysin and water are pressed 1:4500 ratios are diluted, then
Gibberellic acid, alcohol and sodium hypochlorite are added, is uniformly mixed, is modulated into nutrient solution A；By brassinosteroid, 2,3- dichloro-benzenes
After fluoroacetic acid and N- (chloro- 4 pyridine radicals of 3-)-N '-phenylurea mixing, stir, obtain mixed liquor A, then by mixed liquor A and water
By 1:200 ratios are diluted, and nutrient solution B is made；Root-inducing powder and water are pressed 1:750 ratios are carried out after water-soluble dilution, are added
Jasmonic and salicylic acid, are uniformly mixed, and obtain mixed liquid B, then water-loss reducer and mixed liquid B are pressed into 1:The mixing of 150 ratios is adjusted
System, is uniformly mixed, nutrient solution C is made；
(3) prepared by stock：From the wild seedling of walnut as stock, stock is removed into growing point and lobus cardiacus, in two sons
The breach of leaf intermediate vertical down-cutting 7mm length, bevel is smooth；
(4) scion collection and processing：Collection walnut sprout to be grafted is as scion, under scion cotyledon at about 0.9cm
The two-sided wedge shape of 7mm length is whittled into young stem both sides, bevel is smooth；
(5) sterilize：Stock and scion are soaked in nutrient solution A, soak time is 10min；
(6) scion grafting pre-treatment：The scion sterilized is immersed in nutrient solution B, soak time is 2h；
(7) grafting：The stock handled well and scion are subjected to grafting, i.e., scion folding wedge gently inserted in face of quasi- stock otch
Enter, it is ensured that mouthful alignment of scion and stock grafting, make two incisions laminating close, then with Parafilm sealed membranes by scion and stock knot
Close position to tightly wrap in a down-to-earth manner, entered with rainwater-proof；
(8) soak：In the afternoon after 5 points, the walnut nursery stock for starting to graft, which is put into nutrient solution D, to be soaked, during immersion
Between be 10h；
(9) plant：The walnut nursery stock of immersion treatment is transplanted in warmhouse booth, temperature of shed is 23 DEG C, and air is relative
Humidity 75%, after 3 week, then is transplanted to field, and watered, applied fertilizer, the rural area management such as weeding.
Embodiment 2
A kind of walnut nursery stock grafting and breeding method, specifically includes following steps：
(1) raw material selection and its parts by weight：4 parts of alcohol, 2.5 parts of sodium hypochlorite, streptomysin 4 that volumetric concentration is 73%
Part, 4 parts of gibberellic acid, 5 parts of brassinosteroid, 4 parts of 2,3- dichlorphenoxyacetic acids, N- (chloro- 4 pyridine radicals of 3-) 4 parts of-N '-phenylurea,
2.5 parts of 2.5 parts of rosin, 5 parts of jasmonic, 5 parts of salicylic acid, 3-5 parts of root-inducing powder and water-loss reducer；
(2) prepared by nutrient solution：According to parts by weight in step (1), streptomysin and water are pressed 1:5000 ratios are diluted, then
Gibberellic acid, alcohol and sodium hypochlorite are added, is uniformly mixed, is modulated into nutrient solution A；By brassinosteroid, 2,3- dichloro-benzenes
After fluoroacetic acid and N- (chloro- 4 pyridine radicals of 3-)-N '-phenylurea mixing, stir, obtain mixed liquor A, then by mixed liquor A and water
By 1:230 ratios are diluted, and nutrient solution B is made；Root-inducing powder and water are pressed 1:800 ratios are carried out after water-soluble dilution, are added
Jasmonic and salicylic acid, are uniformly mixed, and obtain mixed liquid B, then water-loss reducer and mixed liquid B are pressed into 1:The mixing of 200 ratios is adjusted
System, is uniformly mixed, nutrient solution C is made；
(3) prepared by stock：From the wild seedling of walnut as stock, stock is removed into growing point and lobus cardiacus, in two sons
The breach of leaf intermediate vertical down-cutting 9mm length, bevel is smooth；
(4) scion collection and processing：Collection walnut sprout to be grafted is as scion, under scion cotyledon at about 1.0cm
The two-sided wedge shape of 9mm length is whittled into young stem both sides, bevel is smooth；
(5) sterilize：Stock and scion are soaked in nutrient solution A, soak time is 11min；
(6) scion grafting pre-treatment：The scion sterilized is immersed in nutrient solution B, soak time is 2.5h；
(7) grafting：The stock handled well and scion are subjected to grafting, i.e., scion folding wedge gently inserted in face of quasi- stock otch
Enter, it is ensured that mouthful alignment of scion and stock grafting, make two incisions laminating close, then with Parafilm sealed membranes by scion and stock knot
Close position to tightly wrap in a down-to-earth manner, entered with rainwater-proof；
(8) soak：In the afternoon after 5 points, the walnut nursery stock for starting to graft, which is put into nutrient solution D, to be soaked, during immersion
Between be 11h；
(9) plant：The walnut nursery stock of immersion treatment is transplanted in warmhouse booth, temperature of shed is 25 DEG C, and air is relative
Humidity 80%, after 3.5 week, then is transplanted to field, and watered, applied fertilizer, the rural area management such as weeding.
Embodiment 3
A kind of walnut nursery stock grafting and breeding method, specifically includes following steps：
(1) raw material selection and its parts by weight：Volumetric concentration for 75% 5 parts of alcohol, 3 parts of sodium hypochlorite, 5 parts of streptomysin,
5 parts of gibberellic acid, 6 parts of brassinosteroid, 5 parts of 2,3- dichlorphenoxyacetic acids, N- (chloro- 4 pyridine radicals of 3-) 5 parts of-N '-phenylurea, pine
3 parts of fragrant 3 parts, 6 parts of jasmonic, 6 parts of salicylic acid, 5 parts of root-inducing powder and water-loss reducer；
(2) prepared by nutrient solution：According to parts by weight in step (1), streptomysin and water are pressed 1:5500 ratios are diluted, then
Gibberellic acid, alcohol and sodium hypochlorite are added, is uniformly mixed, is modulated into nutrient solution A；By brassinosteroid, the basic element of cell division,
Frerrous chloride, potassium acetate, after 2,3- dichlorphenoxyacetic acids and N- (chloro- 4 pyridine radicals of 3-)-N '-phenylurea mixing, stir,
Mixed liquor A is obtained, then mixed liquor A and water are pressed 1:250 ratios are diluted, and nutrient solution B is made；Root-inducing powder and water are pressed 1:
850 ratios are carried out after water-soluble dilution, are added jasmonic and salicylic acid, are uniformly mixed, and obtain mixed liquid B, then by water conservation
Agent presses 1 with mixed liquid B:250 ratio hybrid modulations, are uniformly mixed, and nutrient solution C is made；
(3) prepared by stock：From the wild seedling of walnut as stock, stock is removed into growing point and lobus cardiacus, in two sons
The breach of leaf intermediate vertical down-cutting 10mm length, bevel is smooth；
(4) scion collection and processing：Collection walnut sprout to be grafted is as scion, under scion cotyledon at about 1.1cm
The two-sided wedge shape of 10mm length is whittled into young stem both sides, bevel is smooth；
(5) sterilize：Stock and scion are soaked in nutrient solution A, soak time is 12min；
(6) scion grafting pre-treatment：The scion sterilized is immersed in nutrient solution B, soak time is 3h；
(7) grafting：The stock handled well and scion are subjected to grafting, i.e., scion folding wedge gently inserted in face of quasi- stock otch
Enter, it is ensured that mouthful alignment of scion and stock grafting, make two incisions laminating close, then with Parafilm sealed membranes by scion and stock knot
Close position to tightly wrap in a down-to-earth manner, entered with rainwater-proof；
(8) soak：In the afternoon after 5 points, the walnut nursery stock for starting to graft, which is put into nutrient solution D, to be soaked, during immersion
Between be 12h；
(9) plant：The walnut nursery stock of immersion treatment is transplanted in warmhouse booth, temperature of shed is 26 DEG C, and air is relative
Humidity 85%, after 4 week, then is transplanted to field, and watered, applied fertilizer, the rural area management such as weeding.
The applicant has done lot of experiments in process of the test, and experiment sets 2 experiments altogether, and each experiment is provided with some groups,
50 plants of walnut nursery stocks of every group of grafting.Part test is now arranged as follows：
Experiment 1
1st group：Nutrient solution A raw materials are streptomysin, and other processing modes are identical with the embodiment of the present invention 1.
2nd group：Nutrient solution A raw materials are gibberellic acid, and other processing modes are identical with the embodiment of the present invention 1.
3rd group：Nutrient solution A raw materials are sodium hypochlorite, and other processing modes are identical with the embodiment of the present invention 1.
4th group：Nutrient solution A raw materials are streptomysin, gibberellic acid, and other processing modes are identical with the embodiment of the present invention 1.
5th group：Nutrient solution A raw materials are streptomysin, sodium hypochlorite, and other processing modes are identical with the embodiment of the present invention 1.
6th group：Embodiments of the invention 1.
7th group：Embodiments of the invention 2.
8th group：Embodiments of the invention 3.
Experiment 2
1st group：Nutrient solution B raw materials are brassinosteroid, and other processing modes are identical with the embodiment of the present invention 1.
2nd group：Nutrient solution B raw materials be 2,3- dichlorphenoxyacetic acids, other processing modes with the phase of the embodiment of the present invention 1
Together.
3rd group：Nutrient solution B raw materials be N- (chloro- 4 pyridine radicals of 3-)-N '-phenylurea, other processing modes with the present invention
Embodiment 1 is identical.
4th group：Nutrient solution B raw materials be brassinosteroid, 2,3- dichlorphenoxyacetic acids, other processing modes with the present invention
Embodiment 1 is identical.
5th group：Nutrient solution B raw materials are brassinosteroid, N- (chloro- 4 pyridine radicals of 3-)-N '-phenylurea, other processing modes
It is identical with the embodiment of the present invention 1.
6th group：Embodiments of the invention 1.
7th group：Embodiments of the invention 2.
8th group：Embodiments of the invention 3.
The growing state for the walnut nursery stock planted daily in observation greenhouse, and record, test 1 statistical result as shown in table 1,
Test 2 statistical results as shown in table 2.
The walnut nursery stock of table 1 passes through the growing state that nutrient solution A is handled
The walnut nursery stock of table 2 passes through the growing state that nutrient solution B is handled
| Group | Wound healing time/day | Do not emerge/% | Huang Miao/% | Dead seedling/% after emerging |
| 1st group | 12.0 | 11.0 | 12.0 | 11.0 |
| 2nd group | 13.5 | 12.5 | 12.5 | 10.5 |
| 3rd group | 12.3 | 11.3 | 11.3 | 9.3 |
| 4th group | 11.2 | 10.2 | 10.2 | 8.2 |
| 5th group | 10.3 | 9.3 | 9.3 | 7.3 |
| 6th group | 4.0 | 6.0 | 5.0 | 6.0 |
| 7th group | 3.5 | 5.3 | 4.5 | 5.5 |
| 8th group | 3 | 4.5 | 4.0 | 5.0 |
In table 1, from the 1st group to the 5th group it was found from, single addition streptomysin, gibberellic acid, sodium hypochlorite are any in nutrient solution A
The effect that kind material does not add any two materials is good, and the effect for adding any two materials is also combined without three kinds of materials
Effect it is good, only three is scientifically combined, walnut seedling growth could be more obviously promoted, significantly shorten wound
Mouthful healing time, improves the survival rate of walnut nursery stock, but compared with the 6th group, effect is substantially reduced, and from the 6th, 7,8 groups
Understand, walnut nursery stock wound healing time, do not emerge, yellow seedling and after emerging dead seedling with nutrient solution material content increase and imitate
Fruit is better.
In table 2, from the 1st group to the 5th group it was found from, it is single add brassinosteroid, 2,3- dichlorphenoxyacetic acids, (3- is chloro- by N-
4 pyridine radicals) any material of-N '-phenylurea do not add any two materials effect it is good, and add the effect of any two materials
The effect that fruit is also combined without three kinds of materials is good, only scientifically combines three, could more be obviously promoted core
Peach seedling growth, especially in wound healing, significantly shorten wound healing time, improve the survival rate of walnut nursery stock, but
That effect is substantially reduced compared with the 6th group, and from the 6th, it was found from 7,8 groups, walnut nursery stock wound healing time, do not emerge, it is yellow
Seedling and after emerging dead seedling with material content increase in nutrient solution, effect is better.
Be shown experimentally that, in order that walnut nursery stock wound healing time, do not emerge, yellow seedling and dead seedling is shown after emerging
Writing improves, and the composition of raw materials of walnut nursery stock grafting and breeding method of the invention is the most scientific and reasonable, effectively shortens Juglans
Wooden wound healing time, promotes root growth, shorten walnut nursery stock breeds the time, improves plant resistant power, makes plant
It is significantly improved in many-sides such as disease-resistant, degeneration-resistant and defence, strengthens walnut nursery stock accommodative ability of environment, is reduced disease and invade
Evil, is greatly facilitated walnut plant percent.
Described above is the detailed description for the present invention preferably possible embodiments, but embodiment is not limited to this hair
The equal change or modification change completed under bright patent claim, the technical spirit suggested by all present invention, all should belong to
Cover the scope of the claims in the present invention.
Claims (7)
1. a kind of walnut nursery stock grafting and breeding method, it is characterised in that specifically include following steps：
(1) selection of raw material and its parts by weight：3-5 parts of alcohol, 2-3 parts of sodium hypochlorite, 3-5 parts of streptomysin, 3-5 parts of gibberellic acid,
4-6 parts of brassinosteroid, 3-5 parts of 2,3- dichlorphenoxyacetic acids, N- (chloro- 4 pyridine radicals of 3-)-N ' -3-5 parts of phenylurea, rosin 2-3
Part, 4-6 parts of jasmonic, 4-6 parts of salicylic acid, 3-5 parts of root-inducing powder and 2-3 parts of water-loss reducer；
(2) prepared by nutrient solution：According to parts by weight in step (1), streptomysin and water are pressed 1:4500-5500 ratios are diluted,
Gibberellic acid, alcohol and sodium hypochlorite are added, is uniformly mixed, nutrient solution A is modulated into；By brassinosteroid, 2,3- dichloros
After phenoxy acetic acid and N- (chloro- 4 pyridine radicals of 3-)-N '-phenylurea mixing, stir, obtain mixed liquor A, then by mixed liquor A with
Water presses 1:200-250 ratios are diluted, and are eventually adding rosin, continue to stir, and nutrient solution B is made；By root-inducing powder and water
By 1:750-850 ratios are carried out after water-soluble dilution, are added jasmonic and salicylic acid, are uniformly mixed, obtain mixed liquid B,
Water-loss reducer and mixed liquid B are pressed 1 again:150-250 ratio hybrid modulations, are uniformly mixed, and nutrient solution C is made；
(3) prepared by stock：From the wild seedling of walnut as stock, stock is removed into growing point and lobus cardiacus, in two cotyledons
Between cut breach vertically downward, bevel is smooth；
(4) scion collection and processing：Collection walnut sprout to be grafted is as scion, under scion cotyledon at about 0.9-1.1cm
Two-sided wedge shape is whittled into young stem both sides, bevel is smooth；
(5) sterilize：Stock and scion are soaked in nutrient solution A, soak time is 10-12min；
(6) scion grafting pre-treatment：The scion sterilized is immersed in nutrient solution B, soak time is 2-3h；
(7) grafting：The stock handled well and scion are subjected to grafting, i.e., scion folding wedge gently inserted in face of quasi- stock otch,
Ensure scion and stock grafting mouthful alignment, make two incisions laminating close, then tightly wrapped scion and stock binding site with sealed membrane
In a down-to-earth manner, entered with rainwater-proof；
(8) soak：The walnut nursery stock grafted is put into nutrient solution C and soaked, soak time is 10-12h；
(9) plant：The walnut nursery stock of immersion treatment is transplanted in warmhouse booth, then is transplanted to field planting between field, during field planting
Carry out conventional field management.
2. walnut nursery stock grafting and breeding method according to claim 1, it is characterised in that：In step (1), the alcohol
Volumetric concentration is 70%-75%.
3. walnut nursery stock grafting and breeding method according to claim 1, it is characterised in that：In step (3), the cutting is split
The a length of 7-10mm of mouth.
4. walnut nursery stock grafting and breeding method according to claim 1, it is characterised in that：In step (4), the double wedge
The a length of 7-10mm of shape, matches with stock breach.
5. walnut nursery stock grafting and breeding method according to claim 1, it is characterised in that：In step (7), the sealed membrane
For Parafilm sealed membranes.
6. walnut nursery stock grafting and breeding method according to claim 1, it is characterised in that：In step (9), canopy temperature
For 23-26 DEG C, relative air humidity 75-85%.
7. walnut nursery stock grafting and breeding method according to claim 1, it is characterised in that：In step (9), planted in greenhouse
The time of plant is 3-4 week.
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| CN107493991A (en) * | 2017-09-14 | 2017-12-22 | 苍溪县龙山园林生态绿化有限责任公司 | A kind of walnut method for culturing seedlings |
| CN107721573A (en) * | 2017-10-20 | 2018-02-23 | 成都盛世光华生物科技有限公司 | Rare micro- willow nutrient solution and its application needed for a kind of energy supplement |
| CN110754281A (en) * | 2019-11-22 | 2020-02-07 | 塔里木大学 | Improved variety strong seedling cultivation method for early bearing walnut grafted seedlings |
| CN111937893A (en) * | 2020-08-24 | 2020-11-17 | 岳阳市曙光林业科技有限公司 | Nursery stock healing promoter, preparation method and grafting method |
| CN114375778A (en) * | 2022-01-11 | 2022-04-22 | 海南省农业科学院蔬菜研究所 | Method for reducing growth of aerial roots of cherry tomato grafted seedlings |
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| CN114375778A (en) * | 2022-01-11 | 2022-04-22 | 海南省农业科学院蔬菜研究所 | Method for reducing growth of aerial roots of cherry tomato grafted seedlings |
| CN114375778B (en) * | 2022-01-11 | 2022-12-13 | 海南省农业科学院蔬菜研究所 | Method for reducing growth of aerial roots of cherry tomato grafted seedlings |
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