Anti- blue light, anti-oxidant, the free based composition and use thereof in packaging of removing, skin care item and preparation method
Technical field
The present invention relates to technical field of skin care, and in particular to a kind of anti-blue light, it is anti-oxidant, remove free based composition and use thereof in packaging,
Skin care item and preparation method.
Background technology
Blue light is a kind of visible ray, and whether natural or artificial all light sources can send blue light, and people are not knowing not
It is exposed in blue light in feel.Blue light can penetrate whole dermal layer of the skin, but human body itself is unable to filter blue light, can not
Synthesis lutein, therefore, how injury of the block blue light to skin becomes particularly important.Lutein in human body skin can be filtered
Fall the blue light of high-energy in natural daylight and indoor light, lutein can also capture the free radical in human skin tissue, delay
Due to aging caused by skin oxidative.
Lutein is a kind of antioxidant of excellent performance, causes cell to be damaged with organ in human body with free radical is resisted
The function of wound.There are some researches show natural antioxidant lutein can prevent the skin lesion caused by the harmful light of the sun.
Lutein is a kind of natural pigment being widely present in vegetables, flowers, fruit and some algae bios, with various biological living
Property, it is widely used, it is one of current international research focus.For example, marigold just contains abundant lutein.
At present, on the market anti-blue light, it is anti-oxidant, remove free based composition and use thereof in packaging and skin care item effect is not obvious, and appearance
Easily cause allergic phenomena.Therefore, which kind of plant material is selected, and being applied in skin care item for its safety is reached into anti-blue light, is resisted
Oxidation, removing free radical effect, are to be badly in need of assistant officer's problem to be solved.
The content of the invention
The purpose of the present invention is to overcome above-mentioned technical problem, there is provided a kind of anti-blue light, anti-oxidant, removing free radical combination
Thing, skin care item and preparation method.
Technical scheme is as follows:
A kind of anti-blue light, it is anti-oxidant, remove free based composition and use thereof in packaging, including following mass percent component:Marigold flower
Compound 0.1-5%, the tea extraction 1-10% and edelweiss extract 1-10% of extract and safflower seed oil.
Marigold flower extract:Marigold contains abundant lutein, and lutein is a kind of antioxidant of excellent performance,
It is added in skin care item, the blue light of high-energy in natural daylight and indoor light can be filtered out, block blue light is to skin
Injury, moreover it is possible to capture the free radical in human skin tissue, delay due to aging caused by skin oxidative.
Safflower seed oil:In safflower seed oil be rich in vitamin E, as a kind of natural, it to human body cell divide,
Anti-aging important role, there is effect well in terms of skin care:Can pore refining, alleviation dry skin, delaying skin
Aging, skin whitening repairs the skin of sunburn, moreover it is possible to prevent the generation of cutaneum carcinoma, is adapted to various skin quality.
Because marigold flower extract is oil-soluble, itself and stability safflower seed oil higher are combined, improve stabilization
Property.
Tea extraction:Main component is Tea Polyphenols, is applied in skin care item, and skin can be made tender smooth, is also helped
In cleaning skin, dark sore is prevented, and can all contain animal and plant fat as the antioxidant of skin care item, many skin care item, especially
It is that, containing unsaturated fat, after the time one is grown, grease can occur oxygen under the effect of air, moisture, light and trace metal ion
Change spoiled, discoloration, Tea Polyphenols can with grease autoxidation when the free radical phase separation that generates, chain reaction is cut off, to grease
Become sour to go bad and have obvious abated effect, the quality requirement of skin care item can be ensured.
Edelweiss extract:Edelweiss have height from imperial ability, can resist atrocious weather and other
Growing environment, containing abundant mineral matter, natural whiting composition and aromatising essence in its active component, can gently and deep layer is clear
Clean skin, removes pollution in the grease and pore of epidermis, while skin tense situation of releiving, skin tool is releived, calm, antioxygen
Change and nourish protective effect.The active ingredient for extracting edelweiss is applied in daily skin care item, can effectively be releived, calm flesh
Skin, harmful substance in discharge skin, there is preferably whitening, moisturizing, moisturizing and anti-wrinkle effect, can give the thoughtful nourishing of skin and breathe out
Shield, allows skin to keep lustrous and transparent white tender and lovely for a long time.
Preferably, the anti-blue light, anti-oxidant, the free based composition and use thereof in packaging of removing include the component of following mass parts:Marigold
Flower extract and compound 0.1-1 parts, tea extraction 1-10 parts and 1-10 parts of the edelweiss extract of safflower seed oil.
Preferably, in the compound of the marigold flower extract and safflower seed oil, Flos Tagetis Erectae extract and safflower seed oil
Mass ratio be 1:19.
Preferably, the preparation method of the compound of the marigold flower extract and safflower seed oil comprises the following steps:
Marigold flower dry powder and n-hexane are pressed 1:3 ratios mix, and extract 3-6 times repeatedly, and after standing 30min, ultrasound is shaken
10min is swung, upper layer of extraction liquid is poured out after standing;
Merge the extract after extracting repeatedly, filtering, filtrate is depressurized rotary evaporation to without distillate at 38 DEG C, obtained
To the grease that brown color is sticky;
The grease and safflower seed oil are pressed 1:19 ratios are well mixed.
The present invention also provides a kind of anti-blue light, anti-oxidant, removing free radical skin care item, and it includes following mass percent
Component:
B phases:Anti- blue light, anti-oxidant, the free based composition and use thereof in packaging 2.1-25% of removing;
C phases:Phenoxyethanol, Chlorphenesin and glycerine 0.1-0.5%
Aminomethyl propanol 0.1-3%
Essence 0.01-1%;
Wherein described anti-blue light, anti-oxidant, the free based composition and use thereof in packaging of removing include the component of following mass parts:Marigold flower is carried
Take compound 0.1-5 parts, tea extraction 1-10 parts and 1-10 parts of the edelweiss extract of thing and safflower seed oil.
Preferably, the anti-blue light, anti-oxidant, the free based composition and use thereof in packaging of removing include the component of following mass parts:Marigold
Flower extract and compound 0.1-1 parts, tea extraction 1-10 parts and 1-10 parts of the edelweiss extract of safflower seed oil.
Preferably, in the compound of the marigold flower extract and safflower seed oil, Flos Tagetis Erectae extract and safflower seed oil
Mass ratio be 1:19.
The present invention also provides a kind of anti-blue light, preparation method that is anti-oxidant, removing free radical skin care item simultaneously, including as follows
Step:
Step S1, by the glycerine of formula ratio, butanediol, EDETATE SODIUM, niacinamide, Natrulon H-10, panthenol, caprylic/capric
Glyceride type Natrulon H-10 esters, Carbomer and deionized water are added and are stirred under vacuum in pot, are heated to 80 DEG C of dissolvings completely, are gone out
Bacterium, obtains D phases;
Step S2, by the cetostearyl alcohol of formula ratio, cetearyl glucoside, docosyl alcohol, the stearic acid of Natrulon H-10 five
Ester and stearoyl dilactic acid sodium, stearic acid, cetostearyl alcohol, Butyrospermum parkii fruit fat, ethylhexyl palmitate, methyl hydroxybenzoate,
Nexbase 2004, triglycerides, dimethyl silicone polymer and tocopherol acetate are added in oil phase pot, are heated to 80 DEG C and have been dissolved
Entirely, A phases are obtained;
Step S3, under agitation, A phases are pumped into vacuum stirring pot, are well mixed, homogeneous 10 minutes;
Step S4, vacuum outgas 20~30 minutes;
Step S5, reduction mixing speed, and cool;
Step S6, when temperature is down to 45 DEG C, add B phases and C phases, be well mixed;
Step S7, discharging, stand, after the assay was approved, filling, packaging, coding, storage.
Preferably, in step S3, mixing speed is 60 revs/min.
Preferably, in step S4, mixing speed is 30 revs/min.
Compared to prior art, anti-blue light, anti-oxidant, the free based composition and use thereof in packaging of removing and the skin care item that the present invention is provided have
Following beneficial effect:
First, the composition includes the compound of marigold flower extract and safflower seed oil, and it can supplement human body skin
In lutein, strengthen human body skin filter blue light ability, injury of the block blue light to skin, moreover it is possible to capture human body skin group
Free radical in knitting, delays due to aging caused by skin oxidative, and safflower seed oil energy pore refining, alleviates dry skin,
Delay skin aging, skin whitening repairs the skin of sunburn, moreover it is possible to prevent the generation of cutaneum carcinoma;
2nd, also include tea extraction in the composition, skin can be made tender smooth, additionally aid cleaning skin, prevent
Dark sore, and the quality become sour rotten, improve skin care item of grease in skin care item as the antioxidant of skin care item, can be slowed down;
3rd, edelweiss extract is also included in the composition, being capable of gentle deep layer cleaning skin, removal epidermis
Grease and pore in pollution, while skin tense situation of releiving, have to skin releive, it is calm, anti-oxidant and nourish protection
Effect.
Specific embodiment
With reference to embodiment, the present invention is described in further detail, but embodiments of the present invention not limited to this.
The present invention provides a kind of anti-blue light, anti-oxidant, the free based composition and use thereof in packaging of removing, its component for including following mass parts:
Marigold flower extract and compound 0.1-5 parts, tea extraction 1-10 parts and edelweiss extract 1-10 of safflower seed oil
Part.
The preparation method of the compound of wherein described marigold flower extract and safflower seed oil comprises the following steps:
Marigold flower dry powder and n-hexane are pressed 1:3 ratios mix, and extract 3-6 times repeatedly, and after standing 30min, ultrasound is shaken
10min is swung, upper layer of extraction liquid is poured out after standing;
Merge the extract after extracting repeatedly, filtering, filtrate is depressurized rotary evaporation to without distillate at 38 DEG C, obtained
To the grease that brown color is sticky;
The grease and safflower seed oil are pressed 1:19 ratios are well mixed.
Above-mentioned composition is applied in skin care item, its formula is shown in Table 1.
The component and weight/mass percentage composition of the skin care item of the embodiment 1-3 of table 1 and comparative example
Above-described embodiment 1-3 and comparative example are prepared by the following preparation method:
Step S1, by the glycerine of formula ratio, butanediol, EDETATE SODIUM, niacinamide, Natrulon H-10, panthenol, caprylic/capric
Glyceride type Natrulon H-10 esters, Carbomer and deionized water are added and are stirred under vacuum in pot, are heated to 80 DEG C of dissolvings completely, are gone out
Bacterium, obtains D phases;
Step S2, by the cetostearyl alcohol of formula ratio, cetearyl glucoside, docosyl alcohol, the stearic acid of Natrulon H-10 five
Ester and stearoyl dilactic acid sodium, stearic acid, cetostearyl alcohol, Butyrospermum parkii fruit fat, ethylhexyl palmitate, methyl hydroxybenzoate,
Nexbase 2004, triglycerides, dimethyl silicone polymer and tocopherol acetate are added in oil phase pot, are heated to 80 DEG C and have been dissolved
Entirely, A phases are obtained;
Step S3, low whipping speed are under conditions of 60 revs/min, A phases to be pumped into vacuum stirring pot, and mixing is equal
It is even, homogeneous 10 minutes;
Step S4, vacuum outgas 20~30 minutes;
Step S5, reduction mixing speed cool to 30 revs/min;
Step S6, when temperature is down to 45 DEG C, add B phases and C phases, be well mixed;
Step S7, discharging, stand, after the assay was approved, filling, packaging, coding, storage.
First, anti-blue light performance detection
Detection method is:By using the survival rate of CellTiter96 (MTS) cells of the detection with metabolic activity, increase
Rate is grown to study damage and of the invention shield of the blue light to people's epidermal keratinocyte (HEK) and human dermis' fibrocyte (HDF)
Protective action of the skin product to the damage.Cell is cultivated in 96- microwell plates, and the cell quantity in each microwell plate is identical.
Will be equipped with the micropore of human epidermal cell and human dermis' fibrocyte packaged has skin care item sample solution of the present invention by one
Flat bottom sterile plate covering, LED (476nm, 1900lux) blue light illumination 9h is being carried out above culture dish 30cm, then
Cell survival rate measure is carried out with CellTiter96 (MTS).
Blank:(37 DEG C) cell is not exposed under blue light at room temperature.
Sample solution is respectively the solution that embodiment 1-3 and the skin care item described in comparative example are dissolved in dimethyl sulfoxide (DMSO).
Three average values of measurement are taken, testing result is to be shown in Table 2.
Table 2
The result of table 2 shows that the skin care item of embodiment 1-3 contain anti-blue light, anti-oxidant, the free based composition and use thereof in packaging of removing, its people
Epidermal keratinocyte activity and human dermis' fibrocyte activity are significantly greater than comparative example and (do not contain anti-blue light, anti-oxidant, removing
Free based composition and use thereof in packaging) activity, illustrate the anti-blue light added in the skin care item of embodiment 1-3, it is anti-oxidant, remove free radical combination
Thing has the ability of stronger filter blue light, being capable of injury of the block blue light to skin.
2nd, antioxygenic property detection
Detection method is:Reactive oxygen species (ROS) are carried out using Oxiselect reactive oxygen species detection kit
Activity and the measured value of oxidative pressure, detect intracellular hydroxyl, the peroxidating produced after blue light illumination under different test conditions
Base and other free radicals.
People's epidermal keratinocyte and human dermis' fibrocyte use DCFH-DA (2 ', 7 '-dichlorofluorescein diethyls on slide
Acid esters) mix, cultivated 1 hour at 37 DEG C.Then, cell is rinsed 2-3 times with D-PBS (phosphate buffered saline (PBS)),
Then the culture medium solution containing sample is added and exposed to blue light (476nm, 1900lux)) 9h under LED.It is last in experiment
Stage, culture medium is replaced by 100 μ L fresh cultures and 100 μ L cell lysis buffer solutions.By after 5min incubation periods, wherein
The culture medium of 150 μ L is added on the microwell plate of black wall -96, tests fluorescence.
Blank:(37 DEG C) cell is not exposed under blue light at room temperature.
Three average values of measurement are taken, testing result is to be shown in Table 3.
Table 3
Sample |
People's epidermal keratinocyte fluorescence intensity |
Human dermis' fibrocyte fluorescence intensity |
Embodiment 1 |
280 |
320 |
Embodiment 2 |
350 |
380 |
Embodiment 3 |
390 |
400 |
Comparative example |
700 |
800 |
Blank |
170 |
190 |
As shown in Table 3, the skin care item of embodiment 1-3 contain anti-blue light, anti-oxidant, the free based composition and use thereof in packaging of removing, its people's table
Cornu cutaneum cell plastid fluorescence intensity and human dermis' fibrocyte fluorescence intensity (do not contain anti-blue light, antioxygen significantly lower than comparative example
Change, remove free based composition and use thereof in packaging) fluorescence intensity, illustrate the anti-blue light of addition in the skin care item of embodiment 1-3, anti-oxidant, clear
Except free based composition and use thereof in packaging has excellent antioxygenic property.
3rd, free radical performance detection is removed
Cleaning Principle is:Free radical is the molecule of high activity, by the biochemical oxidation as a normal cell metabolism part
Reduction reaction is generated or because being generated exposed to environmental factor such as ultraviolet, smoking, pollution and gamma-rays, free radical is once raw
Into internal all of constituent (DNA, cell membrane, protein) will be attacked.DPPH is also known as 1,1- diphenyl -2- trinitro-s
Phenylhydrazine, is a kind of very stable free radical centered on nitrogen, 3 phenyl ring of his stability essentially from Resonance Stabilization action
Spatial obstacle, azygous electronics on the nitrogen-atoms being clipped in the middle is acted in pairs from playing its due electronics.It
Ethanol solution is in purple, there is absorption maximum at 517nm wavelength, and absorbance is linear with concentration.It is added thereto to
During free radical scavenger, DPPH can be combined or substituted, reduce free radical quantity, absorbance diminishes, and solution colour shoals,
The ability for removing free radical can be evaluated whereby.Calculated by detecting the effect of sample removing DPPH at 517nm wavelength
Oxidation resistance.
Detection method is:4.0mL (0.02mg/ml) DPPH (Japanese TOKYO are sequentially added in 10mL colorimetric cylinders
KASEI ethanol solution and 2.0mL each sample absolute ethyl alcohols dilution (3mg/ml)), add absolute ethyl alcohol to scale,
Mix and light absorption value is surveyed at 517nm wavelength with 1cm cuvettes immediately, be designated as Ai, then extinction is surveyed after greenhouse keeps in dark place 30min
Value, is designated as Aj, and check experiment is the ethanol solution for only adding DPPH, and its light absorption value is designated as Ac.It is calculated as follows free radical scavenging activity
(K):
K (%)=[1- (Ai-Aj)/Ac] * 100%
Three average values of measurement are taken, testing result is to be shown in Table 4.
Table 4
From table 4, it can be seen that containing anti-blue light, anti-oxidant, the free based composition and use thereof in packaging of removing, its radicals scavenging in skin care item
Rate illustrates the anti-indigo plant added in skin care item apparently higher than without anti-blue light, skin care item that are anti-oxidant, removing free based composition and use thereof in packaging
Light, anti-oxidant, the free based composition and use thereof in packaging of removing have excellent removing free radical performance.
4th, detected using rear skin elasticity
Detection method is:The skin care item of embodiment 1-3 and comparative example are continuously used 8 weeks through 20 volunteers respectively, are used
Cutometer (Courage&Khazaka) detects skin elasticity R2 values, and statistical analysis is carried out to skin elasticity data.
Skin elasticity R2 values represent that skin elasticity and degree of compacting are better closer to 1, and testing result is shown in Table 5.
Table 5
As known from Table 5, skin care item of the invention add anti-blue light, it is anti-oxidant, remove free based composition and use thereof in packaging after, hence it is evident that can be with
Improve human body skin elasticity.
The composition and skin care item provided compared to prior art, the present invention have the advantages that:
First, the composition includes the compound of marigold flower extract and safflower seed oil, and it can supplement human body skin
In lutein, strengthen human body skin filter blue light ability, injury of the block blue light to skin, moreover it is possible to capture human body skin group
Free radical in knitting, delays due to aging caused by skin oxidative, and safflower seed oil energy pore refining, alleviates dry skin,
Delay skin aging, skin whitening repairs the skin of sunburn, moreover it is possible to prevent the generation of cutaneum carcinoma;
2nd, also include tea extraction in the composition, skin can be made tender smooth, additionally aid cleaning skin, prevent
Dark sore, and the quality become sour rotten, improve skin care item of grease in skin care item as the antioxidant of skin care item, can be slowed down;
3rd, edelweiss extract is also included in the composition, being capable of gentle deep layer cleaning skin, removal epidermis
Grease and pore in pollution, while skin tense situation of releiving, have to skin releive, it is calm, anti-oxidant and nourish protection
Effect.
Embodiments of the invention are the foregoing is only, the scope of the claims of the invention is not thereby limited, it is every to utilize this hair
Equivalent structure or equivalent flow conversion that bright description is made, or directly or indirectly it is used in other related technology necks
Domain, is included within the scope of the present invention.