CN111544343A - Whitening emulsion rich in rosewood essential oil liposome and preparation method and application thereof - Google Patents
Whitening emulsion rich in rosewood essential oil liposome and preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses whitening emulsion rich in rosewood essential oil liposome and a preparation method and application thereof, wherein the whitening emulsion is prepared from the following components in parts by mass: 0.3-5% of emulsifier, 0.001-0.6% of thickener, 0.05-0.6% of antioxidant, 0.5-5.8% of humectant, 0.5-10% of grease, 0.5-2% of lignum dalbergiae odoriferae essential oil liposome and the balance of distilled water, wherein the sum of the mass fractions of the components is 100%; the lignum dalbergiae odoriferae essential oil liposome is prepared from the following components: lecithin, cholesterol, tween-80, lignum Dalbergiae Odoriferae essential oil, and ethanol; the mass ratio of lecithin, cholesterol, tween-80 and the lignum Dalbergiae Odoriferae essential oil is (1-10): (1-5): (1-6): 1; the mass ratio of the lecithin to the ethanol is 1 (20-50); the lignum Dalbergiae Odoriferae essential oil is extracted from lignum Dalbergiae Odoriferae or lignum Dalbergiae Odoriferae. According to the invention, the essential oil of lignum dalbergiae odoriferae is embedded by a liposome technology, and then is added into an emulsion system prepared from other various auxiliary materials, so that the whitening emulsion rich in the lipidosome of the essential oil of lignum dalbergiae odoriferae, which has special effects of diminishing inflammation, fading acne marks, whitening and the like, is obtained.
Description
Technical Field
The invention relates to a daily chemical skin care product technology, in particular to whitening emulsion rich in essential oil liposome of lignum dalbergiae odoriferae and a preparation method and application thereof.
Background
The emulsion system is a milky white liquid skin care product, and has good skin moistening and moisturizing effects because the milky white liquid skin care product is a water-oil balance system. In addition, the emulsion can isolate the dry climate outside, prevent the skin moisture from losing too fast, avoid skin chapping and peeling, and is a necessary skin care product when going out in dry seasons. The special effect type emulsion is added with certain effect components on the basis of the common emulsion to endow the emulsion with a certain special effect. Such as whitening emulsion, has effects of moistening skin and keeping moisture when being applied on the emulsion, and also has whitening effect. The multiple effects of this product also simplify the skin care process and avoid the discomfort associated with multiple products when used in a stacked configuration.
Most whitening skin care products on the market achieve whitening effects mainly through whitening formula design and whitening component stability design, but the whitening components are various in types and different in whitening effects, and the whitening components are easy to oxidize and inactivate or are decomposed under illumination, so that if the whitening skin care products are directly added into the formula, the products are easy to change color and taste, and even the problems of layering and thickening of a liquid system occur, so that the whitening skin care products generally have the problem of uneven whitening effects.
In addition, the Jiangzheng comprises the Dalbergia odorifera and the Dalbergia sanguinea, is a resin-rich wood material of Jiangzheng of leguminous plants, has strong fragrance of flowery odour, fruit fragrance, drug fragrance, honey fragrance, frankincense and the like, is stable and lasting in fragrance, can be used as a fragrance material, has high medicinal value, and has the effects of stopping bleeding, relieving pain, diminishing swelling, promoting granulation, relieving fever, stopping dysentery and the like. Modern researches prove that the aroma of the lignum Dalbergiae Odoriferae mainly comes from volatile oil, and the main components of the oil include limonene, alpha-pinene, beta-ocimene, etc. And the essential oil refined from the lignum Dalbergiae Odoriferae has good effects on color spot, acne and wound repair. At present, the Jiangzhenxiang is mainly used for preparing spices, and the development of products with other effects is still to be developed.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention aims to provide the whitening emulsion rich in the oil liposome of the vacuum essence. According to the invention, the essential oil of lignum dalbergiae odoriferae is embedded by a liposome technology, and then is added into an emulsion system prepared from other various auxiliary materials, so that the lipidosome whitening emulsion rich in the essential oil of lignum dalbergiae odoriferae, which has special effects of diminishing inflammation, fading acne marks, whitening and the like, is obtained.
The invention also aims to provide a preparation method of the whitening emulsion rich in the rosewood essential oil liposome. The preparation process is simple and convenient, the preparation cost is low, the requirement of industrial amplification production can be met, and the prepared emulsion can keep the drug effect, cover partial fragrance and cannot run off.
The invention also aims to provide application of the whitening emulsion rich in the rosewood essential oil liposome in whitening skin care products. The functional essential oil component is prepared into liposome and added into an emulsion system, and the liposome is used as a skin care product and has special effects of diminishing inflammation, fading acne marks, whitening and the like.
One of the purposes of the invention is realized by adopting the following technical scheme: the whitening emulsion rich in the rosewood essential oil liposome is prepared from the following components in parts by mass: 0.3-5% of emulsifier, 0.001-0.6% of thickener, 0.05-0.6% of antioxidant, 0.5-5.8% of humectant, 0.5-10% of grease, 0.5-2% of Jiangzhen essence grease plastid and the balance of distilled water, wherein the sum of the mass fractions of the components is 100%;
the lignum dalbergiae odoriferae essential oil liposome is prepared from the following components: lecithin, cholesterol, tween-80, lignum Dalbergiae Odoriferae essential oil, and ethanol; the mass ratio of the lecithin, the cholesterol, the tween-80 and the lignum dalbergiae odoriferae essential oil is (1-10): (1-5): (1-6): 1; the mass ratio of the lecithin to the ethanol is 1 (20-50).
Further, the lecithin is selected from any one of sunflower seed lecithin, soybean lecithin and egg yolk lecithin or a mixture thereof.
Further, the preparation method of the lignum dalbergiae odoriferae essential oil liposome comprises the following steps:
(1) accurately weighing lecithin, cholesterol and tween-80 in formula amount, placing into a round bottom flask, dissolving lignum Dalbergiae Odoriferae essential oil in ethanol, pouring into the round bottom flask, and performing ultrasonic treatment at 0-8 deg.C for 5-30min under 20-100W for completely dissolving;
(2) removing the dissolved ethanol from the completely dissolved solution in the step (1) by using a reduced pressure rotary evaporation mode, and forming a smooth lipid film on the wall of the round-bottom flask;
(3) adding pure water into the round-bottom flask obtained in the step (2), ultrasonically washing a membrane to obtain a crude liposome solution, treating for 1-3 times under the high-pressure micro-jet of 40-120 Mpa, and freeze-drying to obtain the lignum dalbergiae odoriferae essential oil liposome.
Further, the emulsifier is selected from any one of fatty acid monoglyceride, tween-80, quillaja bark extract, and tea saponin or their mixture.
Further, the thickening agent is selected from any one of sodium alginate, carbomer and xanthan gum or a mixture thereof.
Further, the antioxidant is selected from any one of vitamin C, citric acid and tocopherol or a mixture thereof.
Further, the humectant is selected from any one of hyaluronic acid, pantothenic acid and collagen or a mixture thereof.
Further, the oil is selected from any one of shea butter, grape seed oil, squalane, jojoba oil and olive oil or a mixture thereof.
The second purpose of the invention is realized by adopting the following technical scheme: a preparation method of a whitening emulsion rich in rosewood essential oil liposome comprises the following steps:
(1) adding the emulsifier with the formula amount into distilled water according to the proportion, and uniformly stirring to completely dissolve the emulsifier, thus obtaining a solution 1; the using amount of the distilled water is 20 percent of that of the formula amount of the distilled water;
(2) adding the thickener in the formula amount into distilled water according to the proportion, and uniformly stirring to completely dissolve the thickener, thus obtaining a solution 2; the using amount of the distilled water is 50% of that of the formula amount of the distilled water;
(3) premixing the solution 1 in the step (1) and the solution 2 in the step (2), mixing with grease with a formula amount, dispersing at a high speed for 2-6 min under the conditions of 8000-;
(4) adding the antioxidant, the humectant and the lignum Dalbergiae Odoriferae essential oil liposome into distilled water according to the proportion, and stirring to obtain solution 3; the using amount of the distilled water is 30 percent of that of the formula amount of the distilled water;
(5) and (3) stirring and mixing the emulsion prepared in the step (3) and the solution 3 prepared in the step (4) uniformly at the stirring speed of 800rpm at room temperature to obtain a finished product.
The third purpose of the invention is realized by adopting the following technical scheme: application of the whitening emulsion rich in the dalbergia essential oil liposome in whitening skin care products.
Compared with the prior art, the invention has the beneficial effects that:
the extracted essential oil of the lignum dalbergiae odoriferae is embedded by a liposome technology and further mixed with the emulsion to prepare the emulsion rich in the liposome of the lignum dalbergiae odoriferae essential oil, so that an emulsion product with special effects of diminishing inflammation, fading acne marks, whitening and the like is obtained.
Drawings
FIG. 1 shows the measurement results of antioxidant properties of the essence oil liposome of the present invention;
FIG. 2 shows the effect of the inventive degermed perfume oil liposome on tyrosinase activity;
FIG. 3 shows the effect of the inventive degummed savory oil liposome on melanin content;
FIG. 4 shows the effect of the inventive degummed essence oil liposome on melanin in zebra fish.
Detailed Description
The present invention will be further described with reference to the accompanying drawings and the detailed description, and it should be noted that, in the present invention, the embodiments or technical features described below may be arbitrarily combined to form a new embodiment without conflict.
In the present invention, all the mass fraction ratios are weight units, and the equipment and raw materials used are commercially available or commonly used in the art. The methods in the following examples are conventional in the art unless otherwise specified.
A whitening emulsion rich in lignum Dalbergiae Odoriferae essential oil liposome is prepared from lignum Dalbergiae Odoriferae essential oil liposome as effective component to obtain emulsion preparation with special effects of diminishing inflammation, removing acne mark and whitening skin; the whitening emulsion is prepared from the following components in percentage by mass: 0.3-5% of emulsifier, 0.001-0.6% of thickener, 0.05-0.6% of antioxidant, 0.5-5.8% of humectant, 0.5-10% of grease, 0.5-2% of lignum dalbergiae odoriferae essential oil liposome and the balance of distilled water, wherein the sum of the mass fractions of the components is 100%;
the lignum dalbergiae odoriferae essential oil liposome is prepared from the following components: lecithin, cholesterol, tween-80, lignum Dalbergiae Odoriferae essential oil, and ethanol; the mass ratio of the lecithin, the cholesterol, the tween-80 and the lignum dalbergiae odoriferae essential oil is (1-10): (1-5): (1-6): 1; the mass ratio of the lecithin to the ethanol is 1 (20-50).
The liposome is colloid particle with lipid bilayer membrane structure prepared from phospholipid, and can be used for embedding, carrying and slowly releasing lipophilic, hydrophilic or amphiphilic substances due to the fact that the liposome has a water phase system and an oil phase system at the same time. In addition, the liposome is similar to the cell membrane structure of human body, and the bioactive substances encapsulated in the liposome are easy to be absorbed by human body, thereby increasing the bioavailability of the bioactive substances. According to the invention, the essential oil of lignum dalbergiae odoriferae is embedded by a liposome technology, and then is added into an emulsion system prepared from other various auxiliary materials, so that the lipidosome whitening emulsion rich in the essential oil of lignum dalbergiae odoriferae, which has special effects of diminishing inflammation, fading acne marks, whitening and the like, is obtained; the functional essential oil component is prepared into liposome and added into an emulsion system, so that the drug effect can be kept, partial fragrance can be covered, and the loss is avoided.
The most preferred formulation of the present invention is as follows: calculated by mass fraction (%), 1% of quillaja bark extract, 0.05% of sodium alginate, 0.5% of vitamin C, 0.3% of hyaluronic acid, 5% of shea butter, 2% of lignum dalbergiae odoriferae essential oil liposome, and the balance of distilled water.
As a further preferred embodiment, the lecithin is any one selected from sunflower seed lecithin, soybean lecithin, egg yolk lecithin or a mixture thereof.
As a further preferable scheme, the preparation method of the lignum dalbergiae odoriferae essential oil liposome comprises the following steps:
(1) accurately weighing lecithin, cholesterol and tween-80 in formula amount, placing into a round bottom flask, dissolving lignum Dalbergiae Odoriferae essential oil in ethanol, pouring into the round bottom flask, and performing ultrasonic treatment at 0-8 deg.C for 5-30min under 20-100W for completely dissolving;
(2) removing the dissolved ethanol from the completely dissolved solution in the step (1) by using a reduced pressure rotary evaporation mode, and forming a smooth lipid film on the wall of the round-bottom flask;
(3) adding pure water into the round-bottom flask obtained in the step (2), ultrasonically washing a membrane to obtain a crude liposome solution, treating for 1-3 times under the high-pressure micro-jet of 40-120 Mpa, and freeze-drying to obtain the lignum dalbergiae odoriferae essential oil liposome.
The preparation method of the lignum dalbergiae odoriferae essential oil is an independent essential oil research and development process, which is described in journal 'tropical crop science newspaper' 2018,39 (4): 791-796, comprising the following steps: the lignum Dalbergiae Odoriferae essential oil is prepared from Dalbergia sanguinea Hance or Dalbergia odorifera Hance of Dalbergia of LeguminosaeWood is prepared by pulverizing and supercritical CO2Prepared by extraction and purification processes; wherein the crushed particles are 30-200 meshes; supercritical CO2The extraction pressure is 30-55MPa, the temperature is 40-60 ℃, and the extraction time is 1.5-3 h; the purification process is microfiltration with a filter membrane with the aperture of 0.22-1.0 mu m, and the pressure is 0.2-0.5 MPa.
Essential oil for lowering true qi, as a volatile essential oil, often loses its effective components due to its own volatilization after long-term storage. In addition, an excessively strong fragrance may also make some people not readily accept it. The main innovation of the present invention is to embed the lignum dalbergiae odoriferae essential oil in liposome, and to fuse the lignum dalbergiae odoriferae essential oil in the emulsion system and to exist stably. In addition, the efficacy of the lignum dalbergiae odoriferae essential oil mainly has good effects in the aspects of color spots, whelks, wound repair and the like, and the invention also finds that the lignum dalbergiae odoriferae essential oil has a whitening effect, is the discovery of the effect of the full new efficacy provided by the application, and is the most important innovation point of the invention.
As a further preferable scheme, the emulsifier is any one of fatty acid monoglyceride and fatty acid diglyceride, Tween-80, Quillaja saponaria bark extract and tea saponin or a mixture thereof.
As a further preferable scheme, the thickening agent is any one of sodium alginate, carbomer and xanthan gum or a mixture thereof.
As a further preferred scheme, the antioxidant is selected from any one of vitamin C, citric acid and tocopherol or a mixture thereof. Wherein, vitamin C, citric acid and tocopherol are natural antioxidant substances, wherein the vitamin C not only has good antioxidation, but also can play a role in reducing pigmentation and fading color spots; the antioxidant tocopherol, also known as vitamin E, is effective in moisturizing skin.
As a further preferred embodiment, the humectant is selected from any one of hyaluronic acid, pantothenic acid, collagen or a mixture thereof. Hyaluronic acid, pantothenic acid and collagen are good natural moisturizers, wherein hyaluronic acid can improve skin nutrition metabolism besides the moisturizing effect, enables the skin to be tender and smooth, removes wrinkles, increases elasticity and prevents aging, and is a good transdermal absorption enhancer while moisturizing. Pantothenic acid also has effects in promoting normal keratinization of skin, promoting wound healing, and relieving inflammation. Collagen can run off along with the growth of age gradually in the human body, and collagen is close with the material structure on skin layer, can permeate into skin very fast, combines with the water in the stratum corneum, forms a network structure, locks moisture and increases skin compactness, prevents that the pore is big and the wrinkle produces.
Further preferably, the oil and fat is selected from any one of shea butter, grape seed oil, squalane, jojoba oil and olive oil or a mixture thereof. Shea butter, grapeseed oil, squalane, jojoba oil and olive oil are all good natural skin-care lipids. Shea butter can promote epidermal cell regeneration and provide skin with nutrition. The grape seed oil can enhance the moisturizing effect of skin and achieve the effect of moisturizing skin. Squalane is a rare animal oil with extremely high chemical stability and excellent use feeling, can strengthen and repair the epidermis, effectively form a natural protective film and help the balance between the skin and the sebum. Jojoba oil is fresh and fine, can be rapidly absorbed by skin, and can soften cutin and make skin smooth and elastic. The olive oil contains squalene, essential fatty acid of human body and some antioxidant substances, and can be rapidly absorbed, effectively maintain skin elasticity and moistening, eliminate facial wrinkle, and prevent skin aging and rhagadia manus et pedis.
The second purpose of the invention is realized by adopting the following technical scheme: a preparation method of a whitening emulsion rich in rosewood essential oil liposome comprises the following steps:
(1) adding the emulsifier with the formula amount into distilled water according to the proportion, and uniformly stirring to completely dissolve the emulsifier, thus obtaining a solution 1; the using amount of the distilled water is 20 percent of that of the formula amount of the distilled water;
(2) adding the thickener in the formula amount into distilled water according to the proportion, and uniformly stirring to completely dissolve the thickener, thus obtaining a solution 2; the using amount of the distilled water is 50% of that of the formula amount of the distilled water;
(3) premixing the solution 1 in the step (1) and the solution 2 in the step (2), mixing with grease with a formula amount, dispersing at a high speed for 2-6 min under the conditions of 8000-;
(4) adding the antioxidant, the humectant and the lignum Dalbergiae Odoriferae essential oil liposome into distilled water according to the proportion, and stirring to obtain solution 3; the using amount of the distilled water is 30 percent of that of the formula amount of the distilled water;
(5) and (3) stirring and mixing the emulsion prepared in the step (3) and the solution 3 prepared in the step (4) uniformly at the stirring speed of 800rpm at room temperature to obtain a finished product.
The third purpose of the invention is realized by adopting the following technical scheme: the application of the whitening emulsion rich in the dalbergia essential oil liposome in whitening skin care products comprises but is not limited to whitening emulsion, whitening toner and whitening cream.
The following are specific examples of the present invention, and raw materials, equipments and the like used in the following examples can be obtained by purchasing them unless otherwise specified.
Example 1: whitening emulsion rich in rosewood essential oil liposome
The whitening emulsion containing the lipid rich in the essential oil of lignum dalbergiae odoriferae is prepared from the following components in parts by weight: 1g of emulsifier, 0.05g of thickener, 0.3g of antioxidant, 1.5g of humectant, 5g of grease, 0.5g of lignum dalbergiae odoriferae essential oil liposome and 92ml of distilled water;
the lignum dalbergiae odoriferae essential oil liposome is prepared from the following components: lecithin, cholesterol, tween-80, lignum Dalbergiae Odoriferae essential oil, and ethanol; the mass ratio of the lecithin, the cholesterol, the tween-80 and the lignum dalbergiae odoriferae essential oil is 4: 3: 2: 1; the mass ratio of the lecithin to the ethanol is 1: 30.
The preparation method comprises the following steps:
(1) accurately weighing 4.0g of soybean lecithin, 3.0g of cholesterol and 2.0g of tween-80, placing in a round-bottom flask, dissolving 1g of lignum Dalbergiae Odoriferae essential oil in 120ml of ethanol, pouring into the round-bottom flask, and performing ultrasonic treatment at 4 deg.C under 40W for 20min to completely dissolve;
(2) removing the dissolved ethanol from the solution completely dissolved in the step (1) by using a reduced pressure rotary evaporation mode to form a smooth lipid film on the wall of the round-bottom flask;
(3) adding 30ml of pure water into a round-bottom flask, ultrasonically washing a membrane to obtain a crude liposome solution, treating for 2 times under the high-pressure micro-jet of 100Mpa, and freeze-drying to obtain the lignum Dalbergiae Odoriferae essential oil liposome;
(4) adding 1.0g of Quillaja saponaria bark extract into 18.4ml of distilled water according to the proportion, and stirring uniformly to completely dissolve the extract, thus obtaining a solution 1;
(5) adding 0.05g of sodium alginate into 46ml of distilled water according to the proportion, and uniformly stirring to completely dissolve the sodium alginate to obtain a solution 2;
(6) premixing the solution 1 and the solution 2, mixing with 5g of jojoba oil, dispersing at high speed for 2min at 15000rpm, pouring into high pressure microjet, homogenizing at 30 deg.C and 100Mpa for 3 times, and preparing to obtain emulsion;
(7) adding 0.3g of vitamin C, 1.5g of hyaluronic acid and 0.5g of lignum Dalbergiae Odoriferae essential oil liposome into 27.6ml of distilled water according to a ratio, and stirring to obtain solution 3;
(8) and (3) stirring and mixing the emulsion prepared in the step (6) and the solution 3 uniformly at room temperature and a stirring speed of 600rpm to obtain a finished product 1.
Example 2: whitening emulsion type toner rich in essential oil liposome
The whitening emulsion type toner rich in the essential oil liposome is prepared from the following components in parts by weight: 0.3g of emulsifier, 0.05g of thickener, 0.05g of antioxidant, 0.5g of humectant, 1.5g of grease, 1g of lignum dalbergiae odoriferae essential oil liposome and 97ml of distilled water;
the lignum dalbergiae odoriferae essential oil liposome is prepared from the following components: lecithin, cholesterol, tween-80, lignum Dalbergiae Odoriferae essential oil, and ethanol; the mass ratio of the lecithin, the cholesterol, the tween-80 and the lignum dalbergiae odoriferae essential oil is 15: 2: 2: 1; the mass ratio of the lecithin to the ethanol is 1: 20.
The preparation method comprises the following steps:
(1) accurately weighing 15g of sunflower seed lecithin, 2g of cholesterol and 2g of tween-80, putting into a round-bottom flask, dissolving 1g of lignum Dalbergiae Odoriferae essential oil in 300ml of ethanol, pouring into the round-bottom flask, and performing ultrasonic treatment at 8 deg.C and 100W for 5min to completely dissolve;
(2) removing the dissolved ethanol from the solution completely dissolved in the step (1) by using a reduced pressure rotary evaporation mode to form a smooth lipid film on the wall of the round-bottom flask;
(3) adding 80ml of pure water into a round-bottom flask, ultrasonically washing a membrane to obtain a crude liposome solution, treating for 3 times under the high-pressure micro-jet of 40Mpa, and freeze-drying to obtain the lignum Dalbergiae Odoriferae essential oil liposome;
(4) adding 0.3g of tea saponin into 19.4ml of distilled water according to the proportion, and uniformly stirring to completely dissolve the tea saponin to obtain a solution 1;
(5) adding 0.005g of xanthan gum into 48.5ml of distilled water according to the proportion, and uniformly stirring to completely dissolve the xanthan gum to obtain a solution 2;
(6) premixing the solution 1 and the solution 2, mixing with 1g of grape seed oil and 0.5g of squalane, dispersing at 8000 for 6min, and homogenizing in high pressure micro jet at 30 deg.C and 120Mpa for 2 times to obtain emulsion;
(7) adding 0.05g of citric acid, 0.5g of pantothenic acid and 1g of lignum dalbergiae odoriferae essential oil liposome into 29.1ml of distilled water according to the proportion, and uniformly stirring to obtain a solution 3;
(8) and (4) stirring and mixing the emulsion prepared in the step (6) and the solution 3 uniformly at the stirring speed of 800rpm at room temperature to obtain a finished product 2.
Example 3: whitening emulsion rich in rosewood essential oil liposome
The whitening emulsion containing the lipid rich in the essential oil of lignum dalbergiae odoriferae is prepared from the following components in parts by weight: 2g of emulsifier, 0.5g of thickener, 0.6g of antioxidant, 3g of humectant, 10g of grease, 2g of lignum dalbergiae odoriferae essential oil liposome and 82ml of distilled water;
the lignum dalbergiae odoriferae essential oil liposome is prepared from the following components: lecithin, cholesterol, tween-80, lignum Dalbergiae Odoriferae essential oil, and ethanol; the mass ratio of the lecithin, the cholesterol, the tween-80 and the lignum dalbergiae odoriferae essential oil is 2: 1: 1: 1; the mass ratio of the lecithin to the ethanol is 1: 50.
The preparation method comprises the following steps:
(1) accurately weighing 2g of egg yolk lecithin, 1g of cholesterol and 1g of tween-80, placing into a round-bottom flask, dissolving 1g of lignum Dalbergiae Odoriferae essential oil in 120ml of ethanol, pouring into the round-bottom flask, and performing ultrasonic treatment at 5 deg.C under 80W for 15min to completely dissolve;
(2) removing the dissolved ethanol from the solution completely dissolved in the step (1) by using a reduced pressure rotary evaporation mode to form a smooth lipid film on the wall of the round-bottom flask;
(3) adding 50ml of pure water into a round-bottom flask, ultrasonically washing a membrane to obtain a crude liposome solution, treating for 1 time under the high-pressure micro-jet of 120Mpa, and freeze-drying to obtain the lignum Dalbergiae Odoriferae essential oil liposome;
(4) adding 2g of mono-diglycerol fatty acid ester into 16.4ml of distilled water according to the proportion, and uniformly stirring to completely dissolve the mono-diglycerol fatty acid ester to obtain a solution 1;
(5) adding 0.5g of carbomer into 41ml of distilled water according to the proportion, and uniformly stirring to completely dissolve the carbomer to obtain a solution 2;
(6) premixing the solution 1 and the solution 2, mixing with 10g shea butter, dispersing at 12000rpm for 3min, and homogenizing in high pressure micro jet at 45 deg.C and 40Mpa for 2 times to obtain emulsion;
(7) adding 0.6g of tocopherol, 3g of collagen and 2g of lignum dalbergiae odoriferae essential oil liposome into 24.6ml of distilled water according to the proportion, and uniformly stirring to obtain a solution 3;
(8) and (3) uniformly stirring and mixing the emulsion prepared in the step (6) and the solution 3 at room temperature and the stirring speed of 800rpm to obtain a finished product 3.
Effect evaluation and Performance detection
The following experimental data of the lipid of lignum dalbergiae odoriferae essential oil were prepared according to the formulation and method of liposome preparation in case 3, wherein the concentration (%) of lignum dalbergiae odoriferae essential oil is the percentage of the total mass of the lipid; the rosewood essential oil concentration (%) of the rosewood essential oil liposome of example 3 was 20%.
1. The total oxidation resistance of the inventive vacuum essence oil liposome is detected.
The total oxidation resistance test method is as follows, and the test results are as follows:
the total antioxidant determination method comprises the following steps: measuring total antioxidant capacity (T-AOC) ELISA kit purchased from xuanya with the product number of XY-SJH-N1264, operating according to the kit specification, preparing reagent 1, reagent 2 and reagent 3 provided in the T-AOC kit into mixed reagent according to the proportion of 10:1:1, accurately absorbing 6 mul of sample solution with different concentrations, adding into a 96-well plate, adding 180 mul of mixed reagent and 18 mul of double distilled water, fully mixing, reacting for 10min, measuring the absorbance A at 593nm1. Using double distilled water as system blank and light absorption value as A0. The total antioxidant capacity is calculated according to a standard curve A which is 0.3154C +0.1291, and the specific formula is as follows:
DPPH clearance measurement method: sucking 2ml of lignum Dalbergiae Odoriferae essential oil lipid with different concentrations, mixing with 2ml of freshly prepared DPPH-ethanol solution (0.1mmol/l), reacting at room temperature in dark for 30min, and measuring light absorption value A at 517nm2And a reaction system blank of replacing the sample solution with a sample solvent and replacing the DPPH-ethanol solution with ethanol. DPPH clearance calculation formula is as follows:
wherein A is0In order to substitute the sample solvent for the absorbance of the sample, A1The absorbance of the DPPH-ethanol solution was replaced by ethanol.
And (3) analyzing the test effect: as shown in fig. 1, for the measurement result of the antioxidant property of the lignum dalbergiae odoriferae essential oil liposome, the total antioxidant activity and dpph.clarity of the liposome are significantly enhanced with the increase of the concentration of the lignum dalbergiae odoriferae essential oil liposome, which indicates that the lignum dalbergiae odoriferae essential oil still has good antioxidant ability after being embedded by the liposome, and the antioxidant ability is enhanced with the increase of the concentration of the lignum dalbergiae odoriferae essential oil liposome.
2. The whitening effect of the inventive vacuum essence oil liposome is detected.
The whitening effect test method is as follows:
zebra fish embryo propagation and collection: wild type AB zebra was kept in a laboratory circulating aquaculture system. The light intensity is kept in the room for 14 h/dark light intensity is controlled for 10h, the room temperature is 26 ℃, and the water temperature is 28 ℃. Obtaining embryos by naturally laying eggs, putting adult zebra fishes into an incubation box according to the male-female ratio of 1:1, separating the male and female parts by using a partition plate, drawing off the partition plate the next day, collecting the eggs after 2 hours, and selecting the embryos which are normally developed for testing.
Zebrafish embryo exposure experiment: observing the generation condition of melanin in zebra fish bodies treated by the liposomes with different concentrations, putting the collected embryos into a 6-well plate containing embryo water, and culturing in a constant-temperature incubator at 28 ℃. Transferring the zebra fish embryos cultured for 24h after fertilization into fresh embryo water containing 2.0% of dalbergia odorifera essential oil (20mg/ml positive control group) and different concentrations of dalbergia odorifera essential oil liposome respectively, continuously culturing for 24h, observing the pigmentation condition in the zebra fish body by using an inverted fluorescence microscope, and photographing and recording.
Determination of tyrosinase activity in zebra fish embryos: respectively collecting the juvenile fish treated by the dalbergia odorifera essential oil and the dalbergia odorifera essential oil liposome for 24h, putting 30 embryos into a centrifuge tube filled with 300 mu l of cold cracking liquid buffer solution, then carrying out ultrasonic treatment for 35s under the ice bath condition, and then centrifuging for 15min at 4 ℃ at 10000 r/min. Mu.l of the mixture was added to a 96-well plate containing 180. mu. l L-DOPA, incubated at 37 ℃ in the dark for 30min, and the absorbance was measured at 475 nm. The relative tyrosinase activity of the blank was 100%, and the tyrosinase activity of the sample was a percentage compared to the blank.
Determination of melanin content in zebra fish embryos: the precipitate obtained by the above centrifugation was dissolved in 300. mu.l NaOH (1mol/l), the mouth of the centrifuge tube was closed, dried at 95 ℃ for 30min, and the absorbance was measured at 405nm with a microplate reader. Relative melanin content-OD of sample group405Blank control OD405×100％。
And (3) analyzing the test effect: as shown in fig. 2, for the effect of the lignum dalbergiae odoriferae essential oil liposome on tyrosinase activity, the results show that the lignum dalbergiae odoriferae essential oil liposome of the present invention can effectively inhibit tyrosinase activity, and the effect of inhibiting tyrosinase activity is also significantly enhanced with the increase of the concentration of the lignum dalbergiae odoriferae essential oil liposome, and 2.0% of the lignum dalbergiae odoriferae essential oil liposome can achieve the effect of inhibiting tyrosinase activity with 2.0% of the positive control group.
As shown in fig. 3, for the influence of the lignum dalbergiae odoriferae essential oil liposome on the melanin content, the result shows that the lignum dalbergiae odoriferae essential oil liposome of the present invention has an obvious effect of inhibiting the production of melanin, the lignum dalbergiae odoriferae essential oil liposome with a concentration of 1.0% can achieve the effect of inhibiting the production of melanin of 2.0% of the positive control group, and the melanin content is only about 45% of that of the blank group.
After the zebra fish embryo is treated by 2.0% of lignum dalbergiae odoriferae essential oil and 1.0% of lignum dalbergiae odoriferae essential oil liposome, as shown in fig. 4, the influence of the lignum dalbergiae odoriferae essential oil liposome on melanin in the zebra fish body is shown, and the result shows that: compared with a blank control group, the growth of the melanin of the zebra fish embryo treated by 2.0 percent of dalbergia odorifera essential oil and 1.0 percent of dalbergia odorifera essential oil liposome is reduced to different degrees. In the blank control group, a small amount of black spots were observed on the upper and lower sides of the spinal column of the zebra fish from a side view, that is, a large amount of melanin pigmentation was observed in the embryo of the zebra fish, and a large amount of pigmentation was observed on the back and the entire spinal column of the embryo of the zebra fish from a back view. After being treated by the 1.0% lignum Dalbergiae Odoriferae essential oil liposome, no matter the observation is performed on the back or side, the decrease of melanin can be observed on the spine and back of zebra fish embryo, and the effect is similar to that of the positive control group 2.0% lignum Dalbergiae Odoriferae essential oil, and the optimal implementation concentration is obtained.
The above embodiments are only preferred embodiments of the present invention, and the protection scope of the present invention is not limited thereby, and any insubstantial changes and substitutions made by those skilled in the art based on the present invention are within the protection scope of the present invention.
Claims (10)
1. The whitening emulsion rich in the rosewood essential oil liposome is characterized by being prepared from the following components in parts by mass: 0.3-5% of emulsifier, 0.001-0.6% of thickener, 0.05-0.6% of antioxidant, 0.5-5.8% of humectant, 0.5-10% of grease, 0.5-2% of lignum dalbergiae odoriferae essential oil liposome and the balance of distilled water, wherein the sum of the mass fractions of the components is 100%;
the lignum dalbergiae odoriferae essential oil liposome is prepared from the following components: lecithin, cholesterol, tween-80, lignum Dalbergiae Odoriferae essential oil, and ethanol; the mass ratio of the lecithin, the cholesterol, the tween-80 and the lignum dalbergiae odoriferae essential oil is (1-10): (1-5): (1-6): 1; the mass ratio of the lecithin to the ethanol is 1 (20-50).
2. The lipid whitening emulsion enriched in essential oils of claim 1, wherein the lecithin is any one or a mixture of sunflower seed lecithin, soybean lecithin, egg yolk lecithin.
3. The lipid whitening emulsion rich in dalbergia wood essential oil of claim 1, wherein the preparation method of the lipid whitening emulsion rich in dalbergia wood essential oil comprises the following steps:
(1) accurately weighing lecithin, cholesterol and tween-80 in formula amount, placing into a round bottom flask, dissolving lignum Dalbergiae Odoriferae essential oil in ethanol, pouring into the round bottom flask, and performing ultrasonic treatment at 0-8 deg.C and 20-100W for 5-30min to completely dissolve;
(2) removing the dissolved ethanol from the completely dissolved solution in the step (1) by using a reduced pressure rotary evaporation mode, and forming a smooth lipid film on the wall of the round-bottom flask;
(3) adding pure water into the round-bottom flask obtained in the step (2), ultrasonically washing a membrane to obtain a crude liposome solution, treating for 1-3 times under the high-pressure micro-jet of 40-120 Mpa, and freeze-drying to obtain the lignum dalbergiae odoriferae essential oil liposome.
4. The liposome whitening emulsion rich in lignum dalbergiae odoriferae essential oil according to any one of claims 1 to 3, wherein the emulsifier is selected from any one of fatty acid monoglyceride, tween-80, quillaja bark extract, tea saponin or a mixture thereof.
5. The lipid whitening emulsion rich in essential oils of claim 1 to 3, wherein the thickener is any one of sodium alginate, carbomer, xanthan gum or a mixture thereof.
6. The liposome whitening emulsion enriched in rosewood essential oil according to any of claims 1 to 3, wherein the antioxidant is selected from any one of vitamin C, citric acid, tocopherol or a mixture thereof.
7. The essential oil-enriched liposome whitening emulsion of any one of claims 1 to 3, wherein the humectant is selected from any one of hyaluronic acid, pantothenic acid, collagen or a mixture thereof.
8. The liposome whitening emulsion rich in rosewood essential oil according to any one of claims 1 to 3, wherein the oil is any one of shea butter, grape seed oil, squalane, jojoba oil, olive oil or a mixture thereof.
9. The preparation method of the whitening emulsion rich in the rosewood essential oil liposome is characterized by comprising the following steps of:
(1) adding the emulsifier with the formula amount into distilled water according to the proportion, and uniformly stirring to completely dissolve the emulsifier to obtain a solution 1; the using amount of the distilled water is 20 percent of that of the formula amount of the distilled water;
(2) adding the thickener in the formula amount into distilled water according to the proportion, and uniformly stirring to completely dissolve the thickener to obtain a solution 2; the using amount of the distilled water is 50% of that of the formula amount of the distilled water;
(3) premixing the solution 1 in the step (1) and the solution 2 in the step (2), mixing with grease with a formula amount, dispersing at a high speed of 8000-;
(4) adding the antioxidant, the humectant and the lignum Dalbergiae Odoriferae essential oil liposome into distilled water according to the proportion, and stirring to obtain solution 3; the using amount of the distilled water is 30 percent of that of the formula amount of the distilled water;
(5) and (3) stirring and mixing the emulsion prepared in the step (3) and the solution 3 prepared in the step (4) uniformly at the stirring speed of 800rpm at room temperature to obtain a finished product.
10. Use of the lipid whitening emulsion rich in lignum dalbergiae odoriferae essential oil according to any one of claims 1-3 and 9 in whitening skin care products.
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| CN112516010A (en) * | 2020-12-02 | 2021-03-19 | 广州巴宝莉化妆品有限公司 | Perfume slow-release liposome, preparation method and application in washing and protecting products |
| CN113876658A (en) * | 2021-11-01 | 2022-01-04 | 仙乐健康科技股份有限公司 | Oil-in-water emulsion composition and method for producing the same |
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