JP2008031049A - Anti-acne bacterium composition - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an anti-acne bacterium composition having excellent antibacterial action on Propionibacterium acnes and excellent in acne-preventing or treating effect. <P>SOLUTION: The anti-acne bacterium composition comprises an extract of Primula sikkimensis Hook. and one or more kinds of materials selected from rose water, Japanese apricot fruit water, lemon fruit water and orange fruit water as active ingredients. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

The present invention relates to an anti-acne bacterium composition having an excellent antibacterial effect against Acne bacterium ( Propionibacterium acnes ).

It is said that acne, which is one of the skin diseases, is associated with the growth of acne bacteria, which are bacteria in the hair follicle. Conventionally, as a treatment method for suppressing the growth of acne bacteria, a bactericidal agent such as chlorhexidine gluconate, triclosan, triclocarban, and pionine has been applied to the affected area as a disinfectant. In recent years, plant extracts and the like have also been studied, and acne preventive cosmetics (see Patent Document 1) containing an extract of the genus Salmonella (see Patent Document 1), a therapeutic agent for acne containing whey and extract of pear (Patent Document 2). An anti-acne composition containing a protein extracted from the genus Propionibacterium (see Patent Document 3), a topical skin preparation containing Proanthocyanidin (see Patent Document 4), cis-6-hexadecenoic acid and isopropylmethylphenol Containing anti-acne bacteria composition (see Patent Document 5), acne improving agent containing Hyssop extract (see Patent Document 6), acne prevention composition containing extracts such as Huang Ren (see Patent Document 7), etc. Is disclosed.

JP-A-6-172152 JP 2001-97842 A JP 2002-316944 A JP 2004-115466 A JP 2004-189656 A JP 2004-262862 A JP-A-2005-537306

  In any of the above-mentioned anti-acne bacteria compositions known hitherto, the effect is not always sufficient, and the development of a better active ingredient has been demanded. Therefore, the present invention has been made in view of the above circumstances, and its object is to provide an anti-acne bacterium composition having an excellent antibacterial action against acne bacterium and excellent in acne prevention or treatment effects. There is.

The inventors of the present invention have made various studies in order to solve the above problems, and have selected from an extract of Primula sikkimensis Hook., Rose water, ume fruit water, lemon fruit water, and orange fruit water. It is found that an antibacterial composition against acne is synergistically enhanced by using one or more of the above in combination, and an anti-acne composition excellent in acne prevention or treatment effects is obtained, thereby completing the present invention. It came to.

  That is, the present invention relates to an extract of Primula sickimensis and an anti-acne fungus composition containing one or more selected from rose water, ume fruit water, lemon fruit water, and orange fruit water as active ingredients. .

  ADVANTAGE OF THE INVENTION According to this invention, it has the antibacterial action outstanding with respect to acne bacteria, and can provide the anti- acne bacteria composition excellent in the acne prevention or treatment effect.

  An embodiment of the present invention will be described.

  Primula sickimensis used in the present invention is a plant that is distributed and grows naturally in a cool climate region in the plateau from Siberia through the Chinese inland to the Himalayas.

  When obtaining an extract of Primula sickimensis, any part of Primula sickimensis flowers, fruits, seeds, leaves, stems, roots, etc. may be used, and the whole plant may be used. Although Primula chicemensis may be used for extraction as it is, considering the extraction efficiency, it is preferable to perform extraction after processing such as shredding, drying, and pulverization. Extraction is performed by immersing in an extraction solvent. In order to increase the extraction efficiency, stirring may be performed, or homogenization may be performed in an extraction solvent. The extraction temperature is suitably about 5 ° C. to the boiling point of the extraction solvent. The extraction time varies depending on the type of extraction solvent and the extraction temperature, but is suitably about 4 hours to 14 days.

  Extraction solvents include water, lower alcohols such as methanol, ethanol, propanol, and isopropanol, polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol, and glycerin, and ethers such as ethyl ether and propyl ether. , Polar organic solvents such as esters such as ethyl acetate and butyl acetate, and ketones such as acetone and ethyl methyl ketone can be used, and one or more of these are selected and used. Further, physiological saline, phosphate buffer, phosphate buffered saline, or the like may be used.

  Extracts from the above solvents of Primula Sickimensis can be used as they are, but the concentrated and dried solids can be dissolved again in water or a polar solvent, or decolorized, deodorized, and deodorized as long as these effects are not impaired. You may use, after refine | purifying salt etc. or performing the fractionation process by column chromatography. For storage, it can be freeze-dried after purification and dissolved in a solvent before use.

  The blending amount of the Primula sickimensis extract in the present invention is generally 0.0001 to 10% by mass with respect to the total amount of the anti-acne bacterium composition, and is preferably 0. 01 to 10% by mass, preferably 0. 0.001 to 5.0 mass%, more preferably 0.01 to 1.0 mass%.

As the rose water used in the present invention, steam-distilled water of Damascus rose ( Rosa damascena Mill) flowers and Centifolia rose ( Rosa centifolia L.) flowers can be used.

Damasukusubara is, in the 16th century in a kind that are said to cross the original species of Rozagarika was introduced in Europe (Rosa gallica) and Rosa phenylene Shea (Rosa phoenicia), are grown as perfume raw materials.

The centifolia rose is said to be a natural hybrid between Damascus rose and white rose ( Rosa alba ), and is cultivated in the Grasse district of France, Morocco in Africa, and the Mediterranean coastal countries.

  In the present invention, steam distillation can be carried out by a method using a known steam distillation apparatus with raw rose flowers. Since these plant bodies contain essential oils in the thin cells of the membrane just below the surface, they can be sufficiently steam distilled without pre-grinding the material as a pretreatment. In general, when extracting essential oils, a method is used in which the material to be distilled is boiled in a state where the material is completely submerged in water. Instead of being immersed in water, it may be placed on the upper grid and heated steam passed through. The steam distilled water of the present invention can be employed by either method.

  The steam-distilled water of the present invention can be obtained by filtering the water layer part finally collected from the obtained fraction. If necessary, treatments such as filtration, deodorization, and decolorization may be added as long as the effect is not affected. Moreover, you may use antiseptic | preservatives, such as a paraoxybenzoic acid ester, so that the obtained steam distilled water may not spoil during long-term storage.

  The amount of the rose water is preferably 0.01 to 10.0% by mass with respect to the total amount of the anti-acne bacteria composition from the viewpoint of anti-acne bacteria power and stability.

  The ume fruit water, lemon fruit water, and orange fruit water used in the present invention are not particularly limited. For example, fruit water such as ume fruit, lemon fruit, orange fruit, etc., cultivated on a commercial basis may be used. it can.

  The ume is a deciduous small tree and is 3 to 5 meters high. Fruits range from as much as 5 grams to over 50 grams.

  Lemon is native to India and spread throughout the world from there. Lemon has a strong growth potential and leaves have a unique aroma. Warm places in winter are suitable for cultivation, with large production areas in the US and the Mediterranean coast. Most of the fruits are egg-shaped or oval-shaped and have a size of 100 to 150 g.

  Orange is a species that has spread from India's Assam region to China, Europe, and the new continent, and has developed a large industry. Many fruits are spherical and have a weight of 150 to 200 g.

  Ume fruit water, lemon fruit water, and orange fruit water used in the present invention may be produced by the following methods, but are not limited to these methods. (A) Separated water produced in a reverse osmosis membrane treatment step in which fruit juice is passed through a tubular semipermeable membrane and pressure is applied to remove water from the tube. (B) Distilled water generated in a heating and concentration step in which the water in the fruit juice is evaporated by heating in a vacuum. (C) Water in which ice is melted in a freezing and concentration process in which fruit juice is frozen and ice is separated.

  About the compounding quantity of the ume fruit water which is fruit water, lemon fruit water, and orange fruit water, it is 0.01-10.0 from the point of an anti-acne microbe power and stability with respect to the whole quantity of an anti-acne microbe composition. Mass% is preferred.

  The anti-acne bacteria composition of the present invention can be provided as a cosmetic, quasi-drug, or pharmaceutical product as an external preparation for skin for the purpose of preventing or treating acne.

  The anti-acne bacteria composition according to the present invention can be provided in various dosage forms such as lotion, emulsion, gel, cream and the like.

  In addition to the above-mentioned essential components, the anti-acne bacteria composition according to the present invention includes oil components, moisturizers, powders, pigments, emulsifiers, solubilizers, which are usually added to pharmaceuticals, quasi drugs, and cosmetics. Ultraviolet absorbers, thickeners, drugs, fragrances, resins, preservatives, antioxidants, alcohols, and the like can be blended within a range that does not interfere with the present invention.

  Further, the features of the present invention will be described in detail by way of examples.

[Preparation Example 1] Primula Shikkimenshisu (Primula sikkimensis Hook.) Dried product was crushed, and was the 1.67kg impregnated week at 50% ethanol 10 times by weight. Thereafter, the residue was removed by filtration, the extract was concentrated under reduced pressure, and further freeze-dried to obtain 0.1 kg of extract, which was designated as Primula sickensis extract.

[Preparation Example 2] After preparing 500 g of fresh flower petals of centifolia rose ( Rosa centifolia L.), adding 2 liters of purified water and immersing for 2 hours, steam distillation was performed as it was submerged in water. The aqueous layer fractionated from the obtained fraction was further filtered to obtain 1000 mL of steam distilled water, which was designated as rose water 1.

[Preparation Example 3] After preparing 500 g of fresh flower petals of Damascus rose ( Rosa damascena Mill), adding 2 liters of purified water and immersing for 2 hours, steam distillation was carried out while still submerged in water. The water layer part fractionated from the obtained fraction was further filtered to obtain 1000 mL of steam distilled water, which was designated as rose water 2.

[Preparation Example 4] 1000 g of a plum ( Prunus mume ) fruit was pressed to obtain a plum juice. The ume fruit juice was concentrated by heating to obtain 100 mL of distilled water, which was used as ume fruit water.

[Preparation Example 5] After 2000 g of lemon ( Citrus limon ) fruit is selected and washed, it is squeezed with a press. Centrifugation removes coarse pulp, wicks, seeds, etc. to obtain purified lemon juice. This lemon juice is poured into a freeze concentrator, frozen at −5 ° C., and separated into ice and concentrated lemon juice by filtration. The ice generated in this step was collected and dissolved to obtain 200 mL of fruit water, which was used as lemon fruit water.

[Preparation Example 6] 1000 g of orange ( Citrus sinensis ) fruit is crushed with a hammer crusher, and the coarse orange is put into a polyester bag and pressed to press it. After sterilization (96 ° C., 30 seconds), coarse pulp and seeds are removed by centrifugation to obtain orange juice. The orange juice is concentrated about twice by applying a pressure of about 50 kg / cm 2 with a reverse osmosis pressure concentrator comprising a cellulose acetate membrane. The membrane-separated water generated in this step was collected to obtain 100 mL of fruit water, which was used as orange fruit water.

  The antibacterial activity against anti-acne bacteria (anti-acne bacteria power) of the present invention was evaluated by the following method.

<Evaluation of anti-acne bacterial power>
1 mL of each sample is weighed on a 24-well microplate (when measuring the synergistic effect of the extract of Primula Sickimensis of the present invention with rose water, plum fruit water, lemon fruit water, orange fruit water, the total amount is Weigh each sample to 1 mL). 0.05 mL of the bacterial solution prepared so that the number of inoculated bacteria becomes 10 ⁶ cfu / g is inoculated per 1 mL of the sample, set in a tube premixer, and cultured with shaking in a 37 ° C. constant temperature bath for 24 hours. Collect 0.1 mL of the bacterial suspension after 24 hours of culture in a test tube dedicated to ATP measurement. Add 0.1 mL of ATP extraction reagent (ATP measurement reagent kit OR-3L1 microorganism extraction reagent, source Organo Corporation) and mix well for 10 seconds. Further, 0.1 mL of a luminescent reagent (ATP measuring reagent kit OR-3L1 luminescent reagent, selling Organo Inc.) was added, and after mixing for 10 seconds, fluorescence intensity was measured with an ATP measuring instrument OR-100 (manufactured by Organo Corporation). . Based on the result of each ATP measurement value, it was evaluated how much each sample has anti-acne bacterial power compared to the solvent control.

The calculation formula is shown below.
Anti-acne bacterial power (%) = 100−S / N × 100
S: luminescence amount of each sample N: luminescence amount of each solvent control The results are shown in Table 1.

  As shown in Table 1, Examples 1 to 9 were used in combination with one or more selected from Primula sikkimensis extract of the present invention and rose water, plum fruit water, lemon fruit water, orange fruit water. Exhibited superior anti-acne fungal activity as compared with Comparative Examples 1 to 6 containing Primula Sikkimmensis extract, rose water, plum fruit water, lemon fruit water and orange fruit water alone. That is, the combined use of Primula sikkimmensis extract and one or more selected from rose water, ume fruit water, lemon fruit water and orange fruit water synergistically prominently exhibits anti-acne fungal activity. It has been shown to improve.

  Next, an Example is shown about the other anti-acne microbe composition of this invention.

[Example 10] Anti-acne fungus composition (lotion type)
(1) Ethanol 5.00 (mass%)
(2) Methyl paraoxybenzoate 0.02
(3) Concentrated glycerin 3.00
(4) 1,3-butylene glycol 1.00
(5) Extract of Primula Sickimensis (Preparation Example 1) 1.00
(6) Rose water 1 (Preparation Example 2) 5.00
(7) Purified water 100.00 The remainder manufacturing method: (1) and (2) It melt | dissolves uniformly and makes it an alcohol phase. This is uniformly mixed with stirring in an aqueous phase which is previously made uniform by adding (3) to (6) to (7). Further, Comparative Example 7 was prepared by substituting (6) for purified water.

Example 11 Anti-Acne Fungal Composition (Emulsion Type)
(1) Glyceryl tri-2-ethylhexanoate 8.00 (mass%)
(2) Self-emulsifying glyceryl monostearate 2.50
(3) Behenyl alcohol 0.50
(4) Concentrated glycerin 5.00
(5) Purified water 100.00 The remainder (6) Xanthan gum (1 wt% aqueous solution) 20.00
(7) Extract of Primula sickimensis (Preparation Example 1) 1.00
(8) Plum fruit water (Preparation Example 4) 10.00
(9) Ethanol 4.00
(10) Methyl paraoxybenzoate 0.15
Production method: The oil phases (1) to (3) and the aqueous phases (4) to (6) are dissolved by heating up to 80 ° C., respectively. Both phases are mixed and uniformly emulsified using a homomixer. After cooling to 45 ° C., the aqueous phases (7) to (8) and the alcohol phase combined with (9) to (10) are added and stirred uniformly. Further, Comparative Example 8 was prepared by substituting (8) for purified water.

[Example 12] Anti-acne fungus composition (cream type)
(1) Squalane 10.00 (mass%)
(2) Stearic acid 2.00
(3) Hydrogenated palm kernel oil 0.50
(4) Hydrogenated soybean phospholipid 0.10
(5) Cetanol 3.60
(6) Lipophilic glyceryl monostearate 2.00
(7) Glycerin 10.00
(8) Methyl paraoxybenzoate 0.10
(9) 1% by weight carboxyvinyl polymer aqueous solution 15.00
(10) Purified water 100.00 The remainder (11) 10 wt% L-arginine aqueous solution 3.00
(12) Lemon fruit water (Preparation Example 5) 5.00
(13) Orange fruit water (Preparation Example 6) 5.00
(14) Extract of Primula sickimensis (Preparation Example 1) 1.00
Production method: The oil phase components (1) to (6) are dissolved by heating to 80 ° C. On the other hand, (7) to (10) are dissolved by heating to 80 ° C. The oil phase is added to this while stirring, then (11) is added, and the mixture is uniformly emulsified with a homogenizer, cooled to 45 ° C., (12) to (14) are added, and the mixture is stirred uniformly. Further, Comparative Example 9 was prepared by substituting (14) for purified water.

The effect of preventing or treating acne of the anti-acne bacteria composition of the present invention was evaluated by the following method.
<Evaluation of acne prevention or treatment effect of anti-acne bacteria composition>
Examples 10 to 12 and Comparative Examples 7 to 9 of the present invention were used blindly, and the effect of improving acne symptoms was evaluated. The evaluation was a group of 20 male and female panelists with acne symptoms who were acne twice a day in the morning and evening for 4 consecutive weeks, acne status before and after use Was visually observed. As a result, the improvement status of acne symptoms was evaluated in four stages of “improvement”, “slight improvement”, “no change”, and “deterioration”, and the number of panelists obtained for each evaluation is shown in Table 2.

As is apparent from Table 2, in the groups using Examples 10 to 12 of the present invention, there was no panelist in which acne symptoms worsened, and an improvement tendency was recognized in 14 or more panelists. On the other hand, in the use groups of Comparative Examples 7 to 9, there were no panelists with improved symptoms, and there were panelists that deteriorated.

Claims (1)

And extracts of Primula Shikkimenshisu (Primula sikkimensis Hook.), Rose water, plum fruit water, lemon fruit water, and one or anti-acne bacteria composition comprising, as an active ingredient, two or more selected from orange fruit water.