CN111407678B - Microemulsion with moisturizing, skin-brightening and anti-aging effects and preparation method thereof - Google Patents

Microemulsion with moisturizing, skin-brightening and anti-aging effects and preparation method thereof Download PDF

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CN111407678B
CN111407678B CN202010352466.3A CN202010352466A CN111407678B CN 111407678 B CN111407678 B CN 111407678B CN 202010352466 A CN202010352466 A CN 202010352466A CN 111407678 B CN111407678 B CN 111407678B
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pomegranate
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fermentation product
moisturizing
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CN111407678A (en
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黄斌斌
胡波
吴小升
潘瑾雯
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Guangzhou Uzen Cosmetics Academy Co ltd
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Guangzhou Uzen Cosmetics Academy Co ltd
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/02Cosmetics or similar toiletry preparations characterised by special physical form
    • A61K8/04Dispersions; Emulsions
    • A61K8/06Emulsions
    • A61K8/068Microemulsions
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/19Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
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    • A61K8/19Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
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    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
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    • A61K8/30Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/40Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
    • A61K8/44Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
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    • A61K8/30Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64Proteins; Peptides; Derivatives or degradation products thereof
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64Proteins; Peptides; Derivatives or degradation products thereof
    • A61K8/645Proteins of vegetable origin; Derivatives or degradation products thereof
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • A61K8/72Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
    • A61K8/73Polysaccharides
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    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9728Fungi, e.g. yeasts
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783Angiosperms [Magnoliophyta]
    • A61K8/9789Magnoliopsida [dicotyledons]
    • AHUMAN NECESSITIES
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    • A61K8/9783Angiosperms [Magnoliophyta]
    • A61K8/9794Liliopsida [monocotyledons]
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
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    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
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    • A61Q19/08Anti-ageing preparations
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    • A61K2800/00Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/85Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine

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Abstract

The invention discloses a microemulsion with the effects of moisturizing, brightening and resisting aging and a preparation method thereof, aiming at providing a microemulsion which is easy to absorb, does not block pores, does not cause secondary damage to skin and has the effects of moisturizing, brightening and resisting aging; the technical scheme is as follows: 0.2-2.0% of squalane, 0.3-3.0% of macadamia nut oil, 0.2-0.5% of shea butter, 0.03-0.15% of sodium hyaluronate, 0.03-0.15% of hydrolyzed sodium hyaluronate, 1.0-3.0% of oat polypeptide, 0.6-1.4% of skin-brightening and anti-aging composite extract, 1.2-5.0% of moisturizing composite extract, 0.5-3.0% of yellow slender root extract, 0.05-0.2% of carnosine, 0.5-5.0% of rose water, 0.2-0.4% of active mineral yeast polypeptide and the like; belongs to the technical field of cosmetics.

Description

Microemulsion with moisturizing, skin-brightening and anti-aging effects and preparation method thereof
Technical Field
The invention relates to a microemulsion, in particular to a microemulsion with a moisturizing, skin-brightening and anti-aging composition; the invention also relates to a preparation method of the emulsion, and belongs to the technical field of cosmetics.
Background
The microemulsion has low oil content, is intermediate products between essence and emulsion, has transparent and exquisite appearance, is easier to absorb because the microemulsion particles are smaller than the emulsion, and develops very rapidly in recent years. Although the swollen micelles also have the advantage of small particles, they have the disadvantage of not having a larger capacity than the microemulsion dispersion, and of having a reduced vapour pressure for volatile essential oils. In addition, the microemulsion is spontaneously formed and stable, so that the microemulsion has more advantages in production and has very wide market prospect.
The prior formula technology mainly comprises a large amount of base oil ester and chemically synthesized raw materials with skin-lightening and anti-aging effects, such as: mineral oil, isopropyl myristate, alpha-arbutin, tranexamic acid, phenethyl resorcinol, retinol and the like have the defects of slow absorption or no absorption, easy pore blockage, large irritation and anaphylaxis, and the efficacy is short and needs to be maintained for long-term use.
Disclosure of Invention
Therefore, the first purpose of the present invention is to provide a microemulsion which is easy to absorb, does not block pores, does not cause secondary damage to the skin, and has the effects of moisturizing, brightening and resisting aging.
A second object of the present invention is to provide a process for the preparation of the above microemulsion.
The first technical scheme provided by the invention is as follows:
a microemulsion with the effects of moisturizing, brightening the skin and resisting aging comprises the following components in percentage by weight: 0.3-1.0% of cetearyl alcohol olive oleate, 0.5-1.0% of sorbitan olive oleate, 0.2-2.0% of squalane, 0.3-3.0% of macadamia integrifolia seed oil, 0.1-0.3% of glyceryl caprylate, 0.2-0.5% of shea butter, 0.3-3.0% of polydimethylsiloxane, 0.05-0.2% of tocopherol, 0.03-0.1% of disodium EDTA, 2.0-5.0% of glycerol, 2.0-8.0% of butanediol, 0.05-0.5% of sodium surfactin, 0.05-0.15% of xanthan gum, 0.5-3.0% of trehalose, 0.5-2.0% of erythritol, 0.03-0.15% of sodium hyaluronate, 0.03-0.15% of hydrolyzed sodium hyaluronate, 0.05-0.25% of acrylate/C10-30 acrylate cross-linked polymer, 0.05-0.25% of arginine, 0.05-0.28% of lauroyl arginine, 0.0.05-1.0.0% of caprylyl ammonia, 0.05-1.0% of caprylyl ammonia, 0.05-3.0% of caprylyl ammonia, 0% of caprylic acid, 0.5-0% of caprylic acid, 0.5-0% of caprylic acid, 0.5-0.5% of caprylic acid, 0.5-0.5% of sodium caprylic acid, 0% of sodium caprylic acid, 0.5-0.5% of sodium caprylic acid, 0% of sodium caprylic acid, 0.5-0.5% of sodium caprylic acid, 0, 1.0-3.0% of oat polypeptide, 0.6-1.4% of skin-brightening and anti-aging compound extract, 1.2-5.0% of moisturizing compound extract, 0.5-3.0% of yellow slender root extract, 0.05-0.2% of carnosine, 0.5-5.0% of rose water, 0.1-0.5% of active mineral yeast polypeptide and 100% of deionized water.
Further, the microemulsion with the effects of moisturizing, brightening and resisting aging comprises the following components in percentage by weight: 0.5-1.0% of cetearyl alcohol olive oleate, 0.6-0.8% of sorbitan olive oleate, 0.5-1.5% of squalane, 1.0-2.0% of macadamia integrifolia seed oil, 0.2% of glyceryl caprylate, 0.3-0.4% of shea butter, 1-2.0% of polydimethylsiloxane, 0.08-0.15% of tocopherol, 0.06-0.8% of disodium EDTA, 3.0-4.0% of glycerol, 3.0-6.0% of butanediol, 0.08-0.3% of sodium subtilopeptide sodium, 0.1-0.12% of xanthan gum, 1-2.0% of trehalose, 0.8-1.6% of erythritol, 0.07-0.10% of sodium hyaluronate, 0.1-0.2% of acrylic acid/C10-30 acrylate cross-linked polymer, 0.1-0.2% of arginine, 0.10-0.20% of hydroxamyl phenylalanine, 0.0.1-2% of caprylyl ammonia, 0.2.0-2% of caprylyl ammonia, 0.0.2% of caprylyl ammonia, 0-1.4% of caprylyl alcohol, 1.5-2.5% of oat polypeptide, 0.8-1.2% of a skin-brightening and anti-aging compound extract, 2.0-4.0% of a moisturizing compound extract, 1-2.0% of a yellow slender root extract, 0.1-0.15% of carnosine, 1-3.0% of rose water, 0.2-0.4% of active mineral yeast polypeptide and 100% of deionized water.
Further, the skin-lightening anti-aging composite extract comprises the following components and contents thereof, wherein the components are calculated according to 100% of the total mass of the microemulsion: 0.2-0.4% of Aureobasidium pullulans fermentation product and 0.2-0.5% of acerola fruit extract.
Furthermore, the skin-lightening and anti-aging composite extract comprises 0.1-0.2% of scutellaria alpina flower/leaf/stem extract and 0.1-0.3% of peucedanum praeruptorum leaf extract according to the total mass of 100% of the microemulsion.
Further, the microemulsion with the effects of moisturizing, brightening the skin and resisting aging is characterized in that the moisturizing compound extract comprises 0.05-0.2% of dendrobium officinale extract, 0.5-2.0% of flos lonicerae/leaf/stem extract, 0.5-3.0% of mallow/leaf/stem extract and 0.1-0.3% of lactobacillus/pomegranate fruit fermentation product extract according to 100% of the total mass of the microemulsion
Further, the micro-emulsion with the effects of moisturizing, brightening and resisting aging is characterized in that the moisturizing composite extract further comprises 0.1-0.3% of lactobacillus/rice fermentation product and 0.5-3.0% of red yeast/rice fermentation product, wherein the total mass of the micro-emulsion is 100%.
Further, the active mineral yeast polypeptide is composed of yeast/zinc fermentation products, yeast/copper fermentation products, yeast/magnesium fermentation products, yeast/iron fermentation products, yeast/silicon fermentation products and leuconostoc/radish root fermentation product filtrate.
Further, in the microemulsion with the effects of moisturizing, brightening and resisting aging, the lactobacillus/pomegranate fruit fermentation product extract is prepared by the following steps in sequence:
1) screening purchased pomegranate fruits, cleaning the pomegranate fruits by using clear water, slicing the pomegranate fruits, placing the pomegranate fruits in a juicer to squeeze and crush pomegranate seeds, and mixing the crushed pomegranate seeds with squeezed pomegranate juice for later use;
2) weighing 300g of the pomegranate mixed solution crushed in the step 1), placing the pomegranate mixed solution in 300ml of distilled water to prepare a pomegranate juice diluted mixed solution, carrying out autoclaving at 121 ℃ for 20min, inoculating 3.0ml of lactobacillus, fermenting for 72 hours, filtering a pomegranate fermentation solution, and collecting filtrate;
3) putting the filtrate obtained in the step) into a freeze dryer for drying for 2H to prepare powder, and crushing the powder to 100 meshes by using a disperser to obtain pomegranate fermentation product for later use;
4) taking 15g of pomegranate fermentation product obtained in the step 3), adding 150ml of distilled water, uniformly mixing, and placing in a constant-temperature water bath kettle to keep 5H at 82-85 ℃;
5) and then centrifuging and filtering the product obtained in the step 4) in a high-speed centrifuge at 5000r/min to obtain filtrate, namely the pomegranate fruit fermentation product extract.
Further, the Bulbilus flavus root extract is prepared by the following steps in sequence:
1) separating the root and the branches and leaves of the slender yellow hearts, and cleaning the slender yellow hearts with clear water;
2) cutting up the root of slender huang, adjusting the temperature of an electric heating constant temperature blast drying box to 80 ℃, putting the cut root of slender huang into the electric heating constant temperature blast drying box, drying for 5H, and crushing to 100 meshes for later use by a crusher after drying;
3) taking 10g of crushed yellow slender root, adding 30ml of butanediol into a triangular flask of 250ml, adding 100ml of deionized water, uniformly mixing, putting into ultrasonic waves for extraction, setting the ultrasonic power at 100W, the time at 150min and the temperature at 25 ℃.
4) Pumping the extracted root extract into a suction filtration bottle by using a vacuum pump to obtain the root extract of the yellow slender heartwood.
The second technical scheme provided by the invention is as follows:
the preparation method of the microemulsion with the effects of moisturizing, brightening and resisting aging sequentially comprises the following steps:
1) weighing each component, and feeding according to A, B, C, D, E, F components, wherein the specific components are as follows:
group A: cetearyl olive oleate, sorbitan olive oleate, squalane, macadamia ternifolia seed oil, caprylic acid ester, shea butter, dimethicone, tocopherol;
group B: deionized water, EDTA disodium, glycerol, butanediol, surfactin sodium, xanthan gum, trehalose, erythritol, sodium hyaluronate, hydrolyzed sodium hyaluronate, acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer and arginine;
group C: undecylenoyl phenylalanine, deionized water, aminomethyl propanol;
group D: 1, 2-hexanediol, octanohydroxamic acid;
group E: oat polypeptide, active mineral yeast polypeptide, skin-brightening and anti-aging compound extract, moisturizing composition, yellow slender root extract and rose water;
and F group: carnosine and deionized water;
2) Weighing the group A raw materials into an oil phase, stirring at a speed of 15r/min, and heating to 78-83 ℃ for later use; weighing the raw materials in the group B into a water phase, stirring at a speed of 25r/min, homogenizing for 3 minutes to obtain a transparent liquid, and heating to 83-85 ℃ for later use; weighing the raw materials in the group C in a container, stirring at the speed of 25r/min for 20min, and controlling the pH value to be 6.2-7.2; weighing the F group raw materials in a container, stirring at a speed of 25r/min for 5min to completely dissolve the powder into clear transparent liquid, and sterilizing and filtering by using a 0.22um filter for later use;
3) pumping the B group of raw materials which are well heated and dissolved into an emulsifying pot by using an emulsifying pot vacuum pump, stirring at the speed of 25r/min, controlling the vacuum pressure at-0.03-0.05 mpa, then slowly pumping the A group of raw materials into the emulsifying pot, stirring at the speed of 30r/min, homogenizing at high temperature for 5min, and keeping the temperature at 80-83 ℃ for 15 min;
4) adding the prepared group C raw materials into an emulsifying pot, stirring at the speed of 25r/min, cooling with the flow rate of cooling water of 0.5H/m3, and vacuumizing to-0.03 mpa;
5) when the temperature of the product in the step 4) is reduced to 48-50 ℃, weighing the group D, uniformly mixing, adding into an emulsifying pot, stirring at the speed of 25r/min, and reducing the temperature;
6) respectively weighing the group E when the temperature of the product in the step 5) is reduced to below 45 ℃, adding the group E into an emulsifying pot, and stirring at the speed of 25r/min for 10min to obtain semitransparent emulsion;
7) adding the prepared F group raw materials into an emulsifying pot, stirring at the speed of 25/min, homogenizing for 1min to obtain semitransparent emulsion, and discharging after the inspection is qualified.
Compared with the prior art, the invention has the following beneficial effects:
1. according to the technical scheme provided by the invention, the undecylenoyl phenylalanine, the active mineral yeast polypeptide, the yellow slender root extract and other components are compounded, so that the generation of melanin can be effectively inhibited, the skin can be effectively lightened for a long time, and the skin aging can be effectively prevented.
2. According to the technical scheme provided by the invention, the undecenoyl phenylalanine, the lactobacillus/pomegranate fruit fermentation product extract, the carnosine, the active mineral yeast polypeptide, the yellow slender root extract and the like are compounded, so that the effects of effectively promoting skin moisture retention and skin brightening, enhancing the activity of cells in a basal layer, keeping skin elasticity and compactness, resisting oxidation, inhibiting the activity of free radicals, adjusting skin immunity, lightening melanin pigmentation, compounding hydrolyzed sodium hyaluronate and the mallow/leaf/stem extract, increasing the water content of the skin, locking water, activating and updating skin cells can be further improved.
4. The technical scheme provided by the invention adopts the composition of undecenoyl phenylalanine, lactobacillus/pomegranate fruit fermentation product extract, carnosine, active mineral yeast polypeptide, fine yellow root extract, acerola fruit extract and the like, through the permeability of active mineral yeast polypeptide trace elements, the combination of the undecenoyl phenylalanine and the lactobacillus/pomegranate fruit fermentation product extract, the extracellular interference of alpha-MSH and MC1R genes reduces the formation of melanin, removes free radicals and removes glycated dark yellow protein; the compound oat polypeptide and the alpine asia fried dough twist/leaf/stem extract can promote the dilution of essential amino acids and trace elements of a human body and improve the skin quality.
5. According to the technical scheme provided by the invention, the undecylenoyl phenylalanine, the active mineral yeast polypeptide, the aureobasidium pullulans fermentation product, the acerola fruit extract, the scutellaria alpina flower/leaf/stem extract, the peucedanum praeruptorum leaf extract, the dendrobium officinale extract, the alpinia canadensis flower/leaf/stem extract, the mallow flower/leaf/stem extract, the lactobacillus/rice fermentation product, the red rice/rice fermentation product and other components are compounded, the undecylenoyl phenylalanine, the lactobacillus/pomegranate fruit fermentation product extract and the yellow slender root extract are combined to form countermeasures with skin melanin by utilizing the active mineral yeast polypeptide trace element permeability, so that the natural antioxidant in the extract permeates into the skin basal layer to clear free radicals, and the skin recovers natural elasticity; the combination of products of malva sylvestris/leaf/stem extract, red yeast rice/rice fermentation and the like effectively prevents various skin problems caused by dry skin.
5. The active mineral yeast polypeptide provided by the technical scheme of the invention consists of yeast/zinc fermentation product, yeast/copper fermentation product, yeast/magnesium fermentation product, yeast/iron fermentation product, yeast/silicon fermentation product and leuconostoc/radish root fermentation product filtrate, is rich in microelements required by cells, supplements microelements required by cell synthetic protein and DNA, has good promotion effect on activity regeneration of skin cells and collagen synthesis, reconstructs skin cells, promotes elastin and renews metabolism of dermis, obviously reduces skin aging signs, relieves damaged skin, and has excellent moisturizing effect.
6. The technical formula provided by the invention is convenient to use and high in absorption speed, and does not block pores to cause skin allergy.
Detailed Description
The following claims are hereby incorporated into the detailed description of the invention, with the understanding that the invention is not to be limited in any way, as any number of modifications may be made by one within the scope of the claims and the invention is still within the scope of the claims.
Unless otherwise specified, the reagents and materials used in the present invention are commercially available or can be prepared by known methods.
Example 1
The invention provides a microemulsion with the effects of moisturizing, brightening and resisting aging, which comprises the following components in percentage by weight: 0.5% of cetearyl olive oleate, 0.5% of sorbitan olive oleate, 0.2% of squalane, 0.3% of macadamia integrifolia seed oil, 0.20% of glyceryl caprylate, 0.2% of shea butter, 0.5% of polydimethylsiloxane, 0.2% of tocopherol (vitamin E), 0.05% of disodium EDTA, 3.0% of glycerol, 7.0% of butanediol, 0.05% of sodium surfactin, 0.05% of xanthan gum, 2.0% of trehalose, 0.5% of erythritol, 0.1% of sodium hyaluronate, 0.08% of hydrolyzed sodium hyaluronate, 0.17% of acrylic acid (ester)/C10-30 alkanol acrylate crosspolymer, 0.2% of arginine, 1.0% of undecylenoyl phenylalanine, 0.08% of aminomethyl propanol, 0.4% of 1, 2-hexanediol, 0.2% of octanoyl hydroxamic acid, 0.2% of mineral hydroxyoxia polypeptide, 1.0% of oat polypeptide, 0.2% of a fermentation product of aureobasidium pullulan, 0.5% of acerola extract, 0.05% of dendrobium officinale extract, 0.15% of lactobacillus/pomegranate fruit fermentation product extract, 0.5% of alpine flower/leaf/stem extract, 2.0% of mallow flower/leaf/stem extract, 0.15% of carnosine, 0.5% of yellow slender root extract, 5.0% of rose water and deionized water to 100%.
Example 2
The invention provides a microemulsion with the effects of moisturizing, brightening and resisting aging, which comprises the following components in percentage by weight: 0.3% of cetearyl olive oleate, 0.5% of sorbitan olive oleate, 0.5% of squalane, 0.4% of macadamia nut oil, 0.15% of glyceryl caprylate, 0.3% of shea butter, 0.3% of polydimethylsiloxane, 0.1% of tocopherol (vitamin E), 0.05% of disodium EDTA, 4.0% of glycerol, 5.0% of butanediol, 0.15% of sodium surfactin, 0.08% of xanthan gum, 1.0% of trehalose, 1.0% of erythritol, 0.12% of sodium hyaluronate, 0.05% of hydrolyzed sodium hyaluronate, 0.19% of acrylic acid (ester)/C10-30 alkanol acrylate crosspolymer, 0.22% of arginine, 1.8% of undecylenoyl phenylalanine, 0.1% of aminomethyl propanol, 0.5% of 1, 2-hexanediol, 0.2% of octanoyl hydroxamic acid, 0.1% of mineral active yeast polypeptide, 2.0% of short-bud fermentation product, 0.4% of acerola aureobasidium glabratum extract, 0.08 percent of dendrobium officinale extract, 1.0 percent of alpine twister/leaf/stem extract, 2.5 percent of lactobacillus/pomegranate fruit fermentation product extract, 3.0 percent of malva sylvestris/leaf/stem extract, 0.1 percent of carnosine, 1.0 percent of slender yellow heart root extract, 4.0 percent of rose water and deionized water to 100 percent.
Example 3
The invention provides a microemulsion with the effects of moisturizing, brightening and resisting aging, which comprises the following components in percentage by weight: 0.5% of cetearyl olive oleate, 1.0% of sorbitan olive oleate, 1.5% of squalane, 0.5% of macadamia nut oil, 0.10% of glyceryl caprylate, 0.5% of shea butter, 1.0% of polydimethylsiloxane, 0.15% of tocopherol (vitamin E), 0.08% of disodium EDTA, 5.0% of glycerol, 4.0% of butanediol, 0.08% of sodium surfactin, 0.10% of xanthan gum, 2.5% of trehalose, 1.5% of erythritol, 0.05% of sodium hyaluronate, 0.05% of hydrolyzed sodium hyaluronate, 0.18% of acrylic acid (ester)/C10-30 alkanol acrylate crosspolymer, 0.21% of arginine, 2.0% of undecylenoyl phenylalanine, 0.1% of aminomethyl propanol, 0.8% of 1, 2-hexanediol, 0.1% of capryloyl hydroxamic acid, 0.5% of mineral hydroxyoxian avena yeast polypeptide, 1.5% of pomegranate polypeptide, 0.5% of a lactobacillus/fruit fermentation product extract, 0.3% of aureobasidium pullulans fermentation product, 0.4% of acerola fruit extract, 0.12% of dendrobium officinale extract, 0.5-3.0% of malva sylvestris/leaf/stem extract, 2.0% of alpine asia flower/leaf/stem extract, 3.0% of rose water, 0.2% of carnosine, 2.0% of yellow slender root extract and 100% of deionized water.
Example 4
The invention provides a microemulsion with the effects of moisturizing, brightening and resisting aging, which comprises the following components in percentage by weight: 0.6% of cetearyl olive oleate, 0.6% of sorbitan olive oleate, 1.5% of squalane, 2.5% of macadamia nut oil, 0.18% of glyceryl caprylate, 0.25% of shea butter, 1.8% of polydimethylsiloxane, 0.05% of tocopherol (vitamin E), 0.06% of disodium EDTA, 3.5% of glycerol, 6.0% of butanediol, 0.5% of sodium surfactin, 0.12% of xanthan gum, 0.50% of trehalose, 2.0% of erythritol, 0.08% of sodium hyaluronate, 0.1% of hydrolyzed sodium hyaluronate, 0.25% of acrylic acid (ester)/C10-30 alkanol acrylate crosspolymer, 0.28% of arginine, 0.5% of undecylenoyl phenylalanine, 0.03% of aminomethyl propanol, 1, 2-hexanediol, 1.0% of capryloyl hydroxamic acid, 0.05% of mineral active yeast polypeptide, 2.5% of wheat bran polypide, 0.3% of short-bud fermentation product, 0.4% of acerola extract, 0.1% of dendrobium officinale extract, 1.5% of alpine twister/leaf/stem extract, 2.0% of lactobacillus/pomegranate fruit fermentation product extract, 0.5% of malva sylvestris/leaf/stem extract, 3.0% of yellow slender root extract, 0.05% of carnosine, 2.0% of rose water and deionized water to 100%.
Example 5
The invention provides a microemulsion with the effects of moisturizing, brightening and resisting aging, which comprises the following components in percentage by weight: 0.7% of cetearyl alcohol olive oleate, 0.6% of sorbitan olive oleate, 1.1% of squalane, 1.3% of macadamia integrifolia seed oil, 0.20% of glyceryl caprylate, 0.4% of shea butter, 1.5% of polydimethylsiloxane, 0.12% of tocopherol (vitamin E), 0.05% of disodium EDTA, 2% of glycerol, 5% of butanediol, 0.10% of sodium surfactin, 0.02% of xanthan gum, 20% of trehalose, 1.2% of erythritol, 0.1% of sodium hyaluronate, 0.08% of hydrolyzed sodium hyaluronate, 0.12% of acrylic acid (ester)/C10-30 alkanol acrylate crosspolymer, 0.12% of arginine, 2% of undecylenoyl phenylalanine, 0.1% of aminomethyl propanol, 0.6% of 1, 2-hexanediol, 0.1% of caprylyl hydroxamic acid, 2% of oat polypeptide, 0.2% of Bremia fermentation product, 0.4% of Carcinia ludiskoides extract, 0.15% of Scutellaria baicalensis flower/leaf/stem extract, 0.2% of peucedanum praeruptorum leaf extract, 0.05% of dendrobium officinale extract, 2.0% of alpine asian flower/leaf/stem extract, 1.2% of malva sylvestris/leaf/stem extract, 0.3% of lactobacillus/pomegranate fruit fermentation product extract, 0.1% of lactobacillus/rice fermentation product, 0.5% of red yeast/rice fermentation product, 2.0% of yellow slender root extract, 0.01% of carnosine, 2% of rose water, 0.2% of active mineral yeast polypeptide and deionized water to 100%.
Example 6
The invention provides a microemulsion with the effects of moisturizing, brightening and resisting aging, which comprises the following components in percentage by weight: 0.5% of cetearyl alcohol olive oleate, 0.8% of sorbitan olive oleate, 1.2% of squalane, 1.5% of macadamia integrifolia seed oil, 0.25% of glyceryl caprylate, 0.35% of shea butter, 2.0% of polydimethylsiloxane, 0.15% of tocopherol (vitamin E), 0.06% of disodium EDTA, 4.0% of glycerol, 3.0% of butanediol, 0.20% of sodium surfactin, 0.10% of xanthan gum, 2.0% of trehalose, 0.8% of erythritol, 0.08% of sodium hyaluronate, 0.12% of hydrolyzed sodium hyaluronate, 0.2% of acrylic acid (ester)/C10-30 alkanol acrylate crosspolymer, 0.2% of arginine, 1.0% of undecylenoyl phenylalanine, 0.2% of aminomethyl propanol, 0.6% of 1, 2-hexanediol, 0.2% of octanoyl hydroxamic acid, 2.0% of oat polypeptide, 0.3% of fermentation product of Bremia brevis, 0.3% of Plumbum preparatium extract, 0.3% of Scutellaria glabra/Scutellaria extract, 0.1% of peucedanum praeruptorum leaf extract, 0.2% of dendrobium officinale extract, 0.5% of alpine asian flower/leaf/stem extract, 3.0% of malva sylvestris/leaf/stem extract, 0.2% of lactobacillus/pomegranate fruit fermentation product extract, 0.15% of lactobacillus/rice fermentation product, 3.0% of red yeast/rice fermentation product, 1.5% of yellow slender root extract, 0.09% of carnosine, 1.0% of rose water, 0.3% of active mineral yeast polypeptide and deionized water to 100%.
Example 7
The invention provides a microemulsion with the effects of moisturizing, brightening and resisting aging, which comprises the following components in percentage by weight: 0.6% of cetearyl alcohol olive oleate, 0.7% of sorbitan olive oleate, 1.8% of squalane, 1.6% of macadamia integrifolia seed oil, 0.16% of glyceryl caprylate, 0.35% of shea butter, 1.5% of polydimethylsiloxane, 0.12% of tocopherol (vitamin E), 0.06% of disodium EDTA, 4.0% of glycerol, 3.0% of butanediol, 0.25% of sodium surfactin, 0.13% of xanthan gum, 2.5% of trehalose, 1.5% of erythritol, 0.1% of sodium hyaluronate, 0.12% of hydrolyzed sodium hyaluronate, 0.15% of acrylic acid (ester)/C10-30 alkanol acrylate crosspolymer, 0.15% of arginine, 1.5% of undecylenoyl phenylalanine, 0.15% of aminomethyl propanol, 0.6% of 1, 2-hexanediol, 0.15% of octanoyl hydroxamic acid, 2.0% of oat polypeptide, 0.4% of fermentation product of Bremia brevicaulis, 0.5% of Plumbum glaucoides extract, 0.2.2% of Scutellaria alpina/Scutellaria alpina extract, 0.3% of peucedanum praeruptorum leaf extract, 0.1% of dendrobium officinale extract, 1.2% of alpine fried dough twist/leaf/stem extract, 0.5% of mallow flower/leaf/stem extract, 0.1% of lactobacillus/pomegranate fruit fermentation product extract, 0.3% of lactobacillus/rice fermentation product, 2.0% of red yeast rice/rice fermentation product, 1.5% of yellow slender root extract, 0.12% of carnosine, 2.5% of rose water, 0.4% of active mineral yeast polypeptide and deionized water to 100%.
The lactobacillus/pomegranate fruit fermentation product extract described in examples 1 to 7, prepared by sequentially:
1) pomegranate fruit is produced in Huaiyuan county in Anhui, China, Lactobacillus (obtained from Lactobacillus) is Shandong micro-on biological science and technology Limited, GT16-3 type desktop high-speed centrifuge (highest rotation speed 16000rpm, Guangzhou Fojie apparatus Limited), FD-2D-80 vacuum freeze dryer (condensation temperature: 80 ℃, vacuum degree: 10Pa, Shanghai Suzhou spectral electronic science and technology Limited), Supor TJE06A vertical juice machine, CF-44 sealing swing type pulverizer (finished product fineness: 80-250mm), rotation speed 25000r/min, Zhejiang Wenling City creative medicinal material apparatus and instruments factory).
2) Screening purchased pomegranate fruits, cleaning with clear water, cutting into pomegranate pieces with a stainless steel knife, placing in a juicer for crushing, and mixing with juice for later use;
3) weighing 300g of crushed pomegranate mixed solution 300ml of distilled water to prepare pomegranate juice mixed solution, performing autoclaving at 121 ℃ for 20min, inoculating 3.0ml of Lactobacillus (Lactobacillus), fermenting for 72 hours, and filtering the pomegranate fermented solution;
4) drying the filtered pomegranate fermentation solution in a freeze dryer for 2H to prepare powder, and crushing the powder to 100 meshes by using a disperser for later use;
5) taking 15g of pomegranate fermentation product, adding 150ml of distilled water, uniformly mixing, and placing in a constant-temperature water bath kettle at 82-85 ℃ for 5H;
6) centrifuging and filtering at a high speed centrifuge of 5000r/min to obtain filtrate, namely pomegranate fruit fermentation product extract.
The Boerhavia diffusa root extract as described in examples 1 to 7, prepared by the following steps in the following order:
1) huang-slender hearts are produced from Yun-nan Yuan-Jiang; butanediol > 99.0% (xylonite, Kabushiki Kaisha); the instrument comprises the following steps: model KQ2200DE digital control ultrasonic cleaner (frequency 40KHZ, sonic equipment ltd, bridge, east guan); SHB-111 circulating water type multipurpose vacuum pump (Zhengzhou great wall science and trade company, Ltd.), DHG-9070A electric heating constant temperature air-blast drying oven (temperature range: RT + -5-250 ℃, Shanghai Heheng instruments & equipments company, Ltd.), CF-44 sealing swing type pulverizer (finished product fineness: 80-250mm), rotation speed 25000r/min, Chujiang Wenling City pioneer medical plant).
2) Separating the root and the branches and leaves of the slender yellow hearts, and cleaning the slender yellow hearts with clear water;
3) cutting up the yellow slender core by a stainless steel knife, adjusting the temperature of an electric heating constant temperature blast drying box to 80 ℃, putting the cut yellow slender core root into the electric heating constant temperature blast drying box, drying for 5H, and crushing to 100 meshes by a crusher for later use;
4) taking 10g of crushed yellow slender root, adding 30ml of butanediol into a triangular flask of 250ml, adding 100ml of deionized water, uniformly mixing, putting into ultrasonic waves for extraction, setting the ultrasonic power at 100W, the time at 150min and the temperature at 25 ℃.
5) Pumping the extracted root extract into a suction filtration bottle by using a vacuum pump to obtain the root extract of the yellow slender heartwood.
Other components were purchased directly, specifically from the merchant, see table 1:
TABLE 1
Name of product Purchasing trade company
Aureobasidium pullulans fermentation product Korea mole
Acerola extract NUTRI-WOODS BIO-TECH (BEIJING) Co.,Ltd.
Scutellaria alpina flower/leaf/stem extract Imperial groups of the netherlands
Peucedanum praeruptorum leaf extract Imperial groups of the netherlands
Dendrobium officinale extract Imperial Dusmann group of the Netherlands
A flos Helianthi/leaf/stem extract Imperial groups of the netherlands
Extract of malva sylvestris leaves/stems Imperial groups of the netherlands
Lactobacillus/Rice fermentation product Imperial groups of the netherlands
Rose water Dali Luhuaxi agricultural science and technology limited
Red Rice/Rice fermentation product NUTRI-WOODS BIO-TECH (BEIJING) Co.,Ltd.
The active mineral yeast polypeptide is prepared by mixing yeast/zinc fermentation product, yeast/copper fermentation product, yeast/magnesium fermentation product, yeast/iron fermentation product, yeast/silicon fermentation product and leuconostoc/radish root fermentation product filtrate according to equal mass ratio, and the raw materials of the active mineral yeast polypeptide all reach the industrial standard.
The preparation method of the microemulsion with the moisturizing, skin-brightening and anti-aging effects described in the embodiment 1 to the embodiment 7 comprises the following steps:
firstly, weighing the components according to the weight ratio in the embodiments 1 to 7, and feeding the components according to A, B, C, D, E, F, wherein the specific components are as follows:
group a (oil phase): cetearyl olive oleate, sorbitan olive oleate, squalane, macadamia ternifolia seed oil, caprylic acid ester, shea butter, dimethicone, tocopherol (vitamin E).
Group B (aqueous phase): deionized water, EDTA disodium, glycerol, butanediol, sodium surfactin, xanthan gum, trehalose, erythritol, sodium hyaluronate, hydrolyzed sodium hyaluronate, acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer and arginine.
Group C (pre-mix 1): undecylenoyl phenylalanine, deionized water, aminomethyl propanol.
Group D: 1, 2-hexanediol, octanohydroxamic acid.
Group E: active mineral yeast polypeptide, oat polypeptide, skin-brightening and anti-aging compound extract, moisturizing composition, yellow slender root extract and rose water.
Group F (pre-mix phase 2): carnosine, deionized water.
The specific implementation production process is as follows:
1) weighing the raw materials of the group A into the oil phase, stirring at a speed of 15r/min, and heating to 78-83 ℃ for later use.
2) Weighing the raw materials of the group B into the water phase, stirring at a speed of 25r/min, homogenizing for 3 minutes at a high speed to obtain a transparent liquid, and heating to 83-85 ℃ for later use.
3) Weighing raw materials of group C (proportion of undecylenoyl phenylalanine to deionized water 1: 5) stirring the mixture in a container for 20min at a speed of 25r/min by using a German IKARW20DS25 laboratory stirrer, and controlling the pH value to be 6.2-7.2.
4) Weighing the raw materials of the F group (carnosine and deionized water ratio 1: 5) stirring with 25r/min speed of German IKARW20DS25 laboratory stirrer for 5min to dissolve the powder completely to obtain clear transparent liquid, sterilizing with 0.22um filter, and filtering.
5) Pumping the B group of raw materials which are well heated and dissolved into an emulsifying pot by using an emulsifying pot vacuum pump, stirring at the speed of 25r/min, controlling the vacuum pressure at-0.03-0.05 mpa, then slowly pumping the A group of raw materials into the emulsifying pot, stirring at the speed of 30r/min, homogenizing at high temperature for 5min, and keeping the temperature at 80-83 ℃ for 15 min;
6) adding the pre-prepared group C raw materials into an emulsifying pot, stirring at a speed of 25r/min, cooling, and allowing the cooling water to flow at a speed of 0.5H/m 3 And vacuumizing to-0.03 mpa.
7) And (3) when the temperature is reduced to 48-50 ℃, weighing the group D, uniformly mixing, adding into an emulsifying pot, and stirring at the speed of 25r/min for cooling.
8) Cooling to below 45 deg.C, respectively weighing group E, adding into emulsifying pan, and stirring at 25r/min for 10min to obtain semitransparent emulsion.
9) Adding the prepared F group raw materials into an emulsifying pot, stirring at the speed of 25/min, and homogenizing for 1min to obtain a semitransparent emulsion.
10) And (5) discharging after the inspection is qualified.
The internal control standard is as follows:
appearance: a translucent or white flowable emulsion;
fragrance: a rose water scent type;
pH value: 6.2 to 7.2
Viscosity (25 ℃, 3#, 30r/min) is more than or equal to 3000 mpa;
stability (cold resistance): keeping 24H in a refrigerator at (-8 +/-2) DEG C, and no layering phenomenon after the temperature is restored to the room temperature;
stability (heat resistance): keeping 24H in a constant temperature box at (40 +/-1) DEG C, and no layering phenomenon exists after the room temperature is recovered;
centrifugal examination: 2000r/min, 30min without layering;
total number of colonies: less than 10 CFU/g;
mold and yeast: less than 10 CFU/g;
heat-resistant coliform group bacteria: cannot be detected;
staphylococcus aureus: cannot be detected;
pseudomonas aeruginosa: and cannot be detected.
To demonstrate the advantages of the solutions provided herein, example 1 (identified as sample 1), example 7 (identified as sample 2), and comparative example 1 (identified as sample 3), comparative example 1 (identified as sample 4), and comparative example 1 (identified as sample 5) of the experimental procedure are described below.
Comparative example 1
The contents of the components and the respective components in this comparative example were the same as those in example 1 except that undecylenoyl phenylalanine, active mineral yeast polypeptide, a yellow slender root extract, an aureobasidium pullulans fermentation product, a acerola fruit extract, a dendrobium officinale extract, a lactobacillus/pomegranate fruit fermentation product extract, a alpine asian flower/leaf/stem extract, a malva sylvestris/leaf/stem extract, and the missing components were supplemented with an equal mass of deionized water.
Comparative example 2
The components and the contents of the respective components in this comparative example were the same as those in example 1 except that aureobasidium pullulans fermentation product, acerola fruit extract, dendrobium officinale extract, lactobacillus/pomegranate fruit fermentation product extract, alpine asia flower/leaf/stem extract, malva sylvestris/leaf/stem extract, 2.0% was absent, and the absent components were supplemented with equal mass of deionized water.
Comparative example 3
The contents of the components and individual components in this comparative example were the same as those in example 1 except that undecylenoyl phenylalanine, the active mineral yeast polypeptide and the yellow slender roots extract were absent and the absent components were supplemented with an equal mass of deionized water.
The specific test method is as follows:
1.1, moisturizing effect: and selecting 20 healthy volunteers with the age of 20-45 years by referring to QB/T4256-2011 cosmetic moisturizing efficacy evaluation guidelines.
1.1.1, test site: in a laboratory evaluation room, the test temperature is 20-22 ℃, and the humidity is 40-60%;
1.1.2, the basic value of a capacitance method skin moisture tester of a forearm test area of a volunteer is 15-60%;
1.1.3 reagents and materials
Paper handkerchief, latex dactylotheca, pipettor.
1.1.4 instruments
Two capacitance method skin moisture apparatus, model: CM825, origin: CK company, Germany; two analytical balances, accuracy 0.1mg, type: BSM-120.4, origin: shanghai Zhuo Jing electronics science and technology, Inc.
1.1.5 measurement procedure
1.1.5 preparation before testing
No cosmetics are used 2-3 days before the test of the tested part, and 2H does not contact water. Before the experiment, the subjects needed to clean the inner forearms of both hands uniformly. The cleaning method is to test the cleaning with a paper towel.
The inner sides of the forearms of both hands of the testee are marked with measuring areas, the area of the testing area is at least 3cm multiplied by 3cm, the same arm can simultaneously mark a plurality of areas, and the interval between each testing area is at least 1 cm.
Volunteers entered the assessment room in advance and sit still for 20min, and can not drink water. The forearm is exposed and placed in a test state to keep relaxed.
1.1.1.6 test requirements
The product application area and the blank control area should be randomly distributed in the left and right arm calibration areas to ensure that the positions of all the products and the blank areas are balanced in a unified theory.
The test sample is (2.0 +/-0.1) mg/cm 2 Is applied in a single application in an amount such thatAnd uniformly smearing the sample in the test area by using a latex finger stall, and recording the actual smearing amount.
After adjusting the apparatus according to the instructions of the capacitance skin moisture meter, the product area and the control area were measured, each area being measured in parallel at least 3 times. The initial values for each test area (before sample application) were measured, and then the skin moisture content of the test and control areas was measured after a set time. The setting time is more than 1H, and a plurality of measuring points such as 2H, 3H and the like can be set according to the product evaluation requirement, and usually not more than 24H.
The test of the same subject must be done by one of his surveyors using the same instrument, and the measuring probe should be cleaned between measurements.
Adverse reactions of the skin of volunteers during the use of the product should be terminated immediately and the volunteers treated appropriately. Adverse reactions were recorded.
The results were evaluated as shown in Table 2.
TABLE 2
Index (I) As a result, the
<30% Is very dry
30~40% Dry matter
40~50% Water-wet
>50% Extremely moist
1.6.1.8 25 volunteers were selected to experience the moisturizing efficacy of samples 1 to 5, with a one week interval between each sample detection. (the following test results are shown in tables 3 to 7, where the test results are average values before, after, and after the test):
TABLE 3 detection results of sample 1
Figure BDA0002472335550000131
Figure BDA0002472335550000141
TABLE 4 detection results of sample 2
Figure BDA0002472335550000142
Figure BDA0002472335550000151
TABLE 5 detection results of sample 3
Figure BDA0002472335550000152
Figure BDA0002472335550000161
Figure BDA0002472335550000171
TABLE 6 detection results of sample 4
Figure BDA0002472335550000172
Figure BDA0002472335550000181
TABLE 7 detection results of sample 5
Figure BDA0002472335550000182
Figure BDA0002472335550000191
1.6.1.9 conclusion is as follows:
according to the test comparison of the microemulsion moisturizing effects of the samples 1 to 5, the samples 1 and 2 have stronger moisturizing effect after being used for 3 hours continuously; samples 3 to 5 returned to the original state soon after use, with sample 3 being the least effective and sample 5 being slightly superior to sample 3 and not having moisturizing effect. Wherein no adverse reaction phenomenon is found in the test.
1.6.2 skin lightening efficacy: and respectively issuing a bottle of respective test use product for each person of the tested volunteers according to the samples 1 and 3, wherein the use period is four weeks, respectively self-photographing one used photo every week for comparison, and performing questionnaire survey after the use of four weeks.
1.6.2.1, sample 1 microemulsion product before use, after four weeks of use, the results of the volunteer questionnaire are shown in table 8, and the test data and calculation results for each subject are shown in table 8.
TABLE 8
Figure BDA0002472335550000192
Figure BDA0002472335550000201
TABLE 8 follow-up questionnaires statistics
Figure BDA0002472335550000202
Figure BDA0002472335550000211
1.6.2.2 sample 3 before and after four weeks of use, the results of the questionnaires from the volunteers are shown in Table 9, and the test data and calculation results for each subject are shown in Table 2.
TABLE 9
Figure BDA0002472335550000212
TABLE 2 follow-up questionnaire statistics
Figure BDA0002472335550000213
Figure BDA0002472335550000221
Figure BDA0002472335550000231
1.6.2.3, conclusion
Compared with the skin brightening effect of the sample 3, the skin brightening effect of the sample 1 is better, the acceptance reaches 96 percent, the skin brightening effect is obvious, and the test effect of the sample 3 is not obvious.
1.6.4, anti-aging effect test: with reference to the Shanghai society for daily chemical industries standard T/SHRH006-2018 cosmetic-free radical (DPPH) clarity test method; the principle is as follows: 1, 1-diphenyl-2-trinitrohydrazine (DPPH for short) is a stable long-life free radical, and the ethanol solution of the stable long-life free radical is dark purple and has strong absorption near 517 nm. In the presence of the free radical scavenger, the DPPH ethanol solution absorbs less light due to single electron pairing. The degree of discoloration of the DPPH ethanol solution is linear with the number of electrons it receives, and thus the ability of the test sample to scavenge free radicals, i.e., the magnitude of antioxidant activity, can be evaluated. The formulations of either set of samples 1 and 3 were validated separately.
1.6.4.1, instrument:
analytical balance: the precision is 0.1 mg; the model is as follows: BSM-120.4, origin: shanghai Zong electronics science and technology, Inc.; test tube: 10 ml; adjustable pipettor: 1.00 ml; ultraviolet visible spectrophotometer, model: 721G-100, wavelength range: 340 + 1000nm, production place: shanghai precision scientific instruments, Inc.
1.6.4.2, reagents
Test water: meets the GB/T6682 regulation of first-grade water; anhydrous ethanol: analytically pure AR 95%; 1, 1-diphenyl-2-trinitrohydrazine (Sigma Co.); vitamin E with purity not less than 96%.
1.6.4.3, 0.12mg/ml ethanol solution of DPPH: weighing 12mg of 1, 1-diphenyl-2-trinitrohydrazine in a 250ml beaker, adding 100ml of 95% ethanol, and stirring and dissolving by using a glass cup until the ethanol is dissolved to obtain the product.
1.6.4.4 Experimental methods
The positive control was dissolved in 95% ethanol and diluted to: a series of concentration gradients of 0.08mg/ml, 0.04mg/ml, 0.02mg/ml, 0.01mg/ml were used to validate the assay system.
1.6.4.5, DPPH free radical clearance calculation formula:
Figure BDA0002472335550000232
(1) in the formula:
t-light absorption value of the sample tube, namely the light absorption value of the solution after the sample reacts with DPPH;
t0-background absorbance of sample;
c-average value of light absorption values of the DPPH tube for 3 times, namely the light absorption value of DPPH solution when no sample is added;
c0-background absorbance of solvent.
1.6.4.6, verification: the test system is considered to be effective if a positive control is required to be added for each experiment, and the IC50 (corresponding to the tested concentration of the sample when 50% of the clearing effect is achieved) of the vitamin E of the positive control is 0.02mg/mL-0.06 mg/mL. And (3) calculating the clearance rate of free radicals of the sample at each tested concentration according to the formula (1), and counting the Standard Deviation (SD) between the clearance rates of each group of parallel tubes, wherein the SD value is less than or equal to 3 percent, and the test parallelism is considered to be effective.
1.6.4.7, sample 1 and sample 3 are diluted with test water to 0.08mg/ml, 0.04mg/ml, 0.02mg/ml and 0.01mg/ml series concentrations respectively;
a10 ml test tube was used to set up sample tubes (T), sample background (T0), DPPH tube (C) and solvent background (C0), with 3 parallel tubes being set up for each sample concentration of sample tubes (T) and 3 parallel tubes being set up for DPPH tube (C).
1mL of the same concentration of sample solution was added to each of the sample tube (T) and sample background (T0).
All tubes (T, T0, C, C0) were filled with solvent, water-soluble sample was added to water, and oil-soluble sample was added to 95% ethanol to make up 3mL, and mixed well.
1mL of an ethanol DPPH solution was added to the sample tube (T) and DPPH tube (C), and the sample background (T0) and the solvent background (C0) were replaced with 95% ethanol, gently shaken, and allowed to stand at room temperature for 5 minutes.
Each reaction solution was transferred to a 1cm cuvette and absorbance was measured at 517 nm.
The test results were as follows:
Figure BDA0002472335550000241
1.6.4.8 conclusion
According to the data of the sample 1 and the sample 3, the analysis shows that the sample 1 has stronger free radical scavenging effect, the content of 0.08mg/ml is as high as 91 percent, and the data of the sample 3 is very low, which indicates that the product has stronger anti-aging effect.
In summary, the disclosure of the present invention is not limited to the above-mentioned embodiments, and those skilled in the art can easily suggest other embodiments within the technical teaching of the present invention, but such embodiments are included in the scope of the present invention.

Claims (5)

1. The microemulsion with the effects of moisturizing, brightening the skin and resisting aging is characterized by comprising the following components in percentage by weight: 0.3-1.0% of cetearyl alcohol olive oleate, 0.5-1.0% of sorbitan olive oleate, 0.2-2.0% of squalane, 0.3-3.0% of macadamia integrifolia seed oil, 0.1-0.3% of glyceryl caprylate, 0.2-0.5% of shea butter, 0.3-3.0% of polydimethylsiloxane, 0.05-0.2% of tocopherol, 0.03-0.1% of disodium EDTA, 2.0-5.0% of glycerol, 2.0-8.0% of butanediol, 0.05-0.5% of sodium surfactin, 0.05-0.15% of xanthan gum, 0.5-3.0% of trehalose, 0.5-2.0% of erythritol, 0.03-0.15% of sodium hyaluronate, 0.03-0.15% of hydrolyzed sodium hyaluronate, 0.05-0.25% of acrylic ester/C10-30 alkanol acrylate cross-linked polymer, 0.05-0.28% of arginine acyl propanol, 0.05-0.0.0.0.03-0% of phenylalanine, 0.3.3.0.3% of methyl 3% of carbene acid, 0.0.2-0.0.3, 0.4-1.0% of 2-hexanediol, 0.05-0.3% of caprylyl hydroximic acid, 1.0-3.0% of oat polypeptide, 0.6-1.4% of a skin-brightening and anti-aging compound extract, 1.75-5.0% of a moisturizing compound extract, 0.5-3.0% of a yellow slender root extract, 0.05-0.2% of carnosine, 0.5-5.0% of rose water, 0.1-0.5% of active mineral yeast polypeptide and 100% of deionized water;
the skin-brightening and anti-aging composite extract comprises the following components in percentage by 100 percent of the total mass of the microemulsion: 0.2-0.4% of Aureobasidium pullulans fermentation product, 0.2-0.5% of acerola fruit extract, 0.1-0.2% of Scutellaria alpina flower/leaf/stem extract and 0.1-0.3% of Peucedanum praeruptorum leaf extract;
according to 100 percent of the total mass of the microemulsion, the components and the contents of the components of the moisturizing composite extract are as follows: 0.05-0.2% of dendrobium officinale extract, 0.5-2.0% of alpine fried dough twist/leaf/stem extract, 0.5-3.0% of mallow flower/leaf/stem extract, 0.1-0.3% of lactobacillus/pomegranate fruit fermentation product extract, 0.1-0.3% of lactobacillus/rice fermentation product and 0.5-3.0% of red yeast/rice fermentation product; moreover, the sum of the contents of all components of the moisturizing composite extract is not more than 5.0 percent;
the active mineral yeast polypeptide consists of yeast/zinc fermentation product, yeast/copper fermentation product, yeast/magnesium fermentation product, yeast/iron fermentation product, yeast/silicon fermentation product and Leuconostoc/radish root fermentation product filtrate.
2. The microemulsion with the effects of moisturizing, brightening and resisting aging according to claim 1 is characterized by comprising the following components in percentage by weight: 0.5-1.0% of cetearyl alcohol olive oleate, 0.6-0.8% of sorbitan olive oleate, 0.5-1.5% of squalane, 1.0-2.0% of macadamia integrifolia seed oil, 0.2% of glyceryl caprylate, 0.3-0.4% of shea butter, 1-2.0% of polydimethylsiloxane, 0.08-0.15% of tocopherol, 0.06-0.08% of disodium EDTA, 3.0-4.0% of glycerol, 3.0-6.0% of butanediol, 0.08-0.3% of sodium subtillide, 0.1-0.12% of xanthan gum, 1-2.0% of trehalose, 0.8-1.6% of erythritol, 0.07-0.10% of sodium hyaluronate, 0.07-0.10% of hydrolyzed sodium hyaluronate, 0.1-0.2% of acrylate/C10-30 alkanol acrylate cross-linked polymer, 0.10-0.2% of arginine, 0.20% of undecyl acid, 1.2.0.0.1-0.1% of phenylalanine, 0.1.1.1% of methyl propanol, 0.1% of methyl amino-1.2% of methyl phenyl ether, 0% of methyl phenyl ether, 0.6-0.8% of 2-hexanediol, 0.1-0.2% of caprylyl hydroximic acid, 1.5-2.5% of oat polypeptide, 0.8-1.2% of a skin-brightening and anti-aging compound extract, 2.0-4.0% of a moisturizing compound extract, 1-2.0% of a yellow slender root extract, 0.1-0.15% of carnosine, 0.2-0.4% of an active mineral yeast polypeptide, 1-3.0% of rose water and 100% of deionized water.
3. The microemulsion with the effects of moisturizing, brightening and resisting aging according to claim 1, wherein the lactobacillus/pomegranate fruit fermentation product extract is prepared by the following steps in sequence:
1) screening purchased pomegranate fruits, cleaning the pomegranate fruits by using clear water, slicing the pomegranate fruits, placing the pomegranate fruits in a juicer to squeeze and crush pomegranate seeds, and mixing the crushed pomegranate seeds with squeezed pomegranate juice for later use;
2) weighing 300g of the pomegranate mixed solution crushed in the step 1), placing the pomegranate mixed solution in 300ml of distilled water to prepare a pomegranate juice diluted mixed solution, carrying out autoclaving at 121 ℃ for 20min, inoculating 3.0ml of lactobacillus, fermenting for 72h, filtering a pomegranate fermentation solution, and collecting filtrate;
3) drying the filtrate obtained in the step 2) in a freeze dryer for 2 hours to prepare powder, and crushing the powder to 100 meshes by using a disperser to obtain pomegranate fermentation products for later use;
4) taking 15g of pomegranate fermentation product obtained in the step 3), adding 150ml of distilled water, uniformly mixing, and placing in a constant-temperature water bath kettle to maintain at 82-85 ℃ for 5 hours;
5) and then centrifuging and filtering the product obtained in the step 4) in a high-speed centrifuge at 5000r/min to obtain a filtrate, namely the lactobacillus/pomegranate fruit fermentation product extract.
4. The microemulsion with the effects of moisturizing, brightening and resisting aging according to claim 1 or 2, wherein the root extract of the slender huangxin is prepared by the following steps in sequence:
1) separating the root and the branches and leaves of the slender yellow hearts, and cleaning the slender yellow hearts with clear water;
2) cutting up the root of slender huang, adjusting the temperature of an electric heating constant temperature blast drying box to 80 ℃, putting the cut root of slender huang into the electric heating constant temperature blast drying box, drying for 5 hours, and crushing to 100 meshes for later use by a crusher after drying;
3) taking 10g of crushed yellow slender root, adding 30ml of butanediol into a triangular flask of 250ml, adding 100ml of deionized water, uniformly mixing, putting into ultrasonic waves for extraction, setting the ultrasonic power at 100W, the time at 150min and the temperature at 25 ℃;
4) pumping the extracted root extract into a suction filtration bottle by using a vacuum pump to obtain the root extract of the yellow slender heartwood.
5. The method for preparing the microemulsion with the effects of moisturizing, brightening and resisting aging as claimed in claim 1, which comprises the following steps in sequence:
1) weighing the components according to the weight percentage of claim 1, and feeding the components according to the A, B, C, D, E and F, wherein the specific components are as follows:
group A: cetearyl olive oleate, sorbitan olive oleate, squalane, macadamia ternifolia seed oil, caprylic acid ester, shea butter, dimethicone, tocopherol;
group B: deionized water, EDTA disodium, glycerol, butanediol, sodium surfactin, xanthan gum, trehalose, erythritol, sodium hyaluronate, hydrolyzed sodium hyaluronate, acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer and arginine;
group C: undecylenoyl phenylalanine, deionized water, aminomethyl propanol;
group D: 1, 2-hexanediol, octanohydroxamic acid;
group E: oat polypeptide, active mineral yeast polypeptide, skin brightening and anti-aging compound extract, moisturizing compound extract, yellow slender root extract and rose water;
and F group: carnosine, deionized water;
2) weighing the raw materials of the group A, stirring and heating at a speed of 15r/min to 78-83 ℃ for later use; weighing the raw materials of the group B, stirring at a speed of 25r/min, homogenizing at a high speed for 3 minutes to obtain transparent liquid, and heating to 83-85 ℃ for later use; weighing the raw materials of the group C, stirring the raw materials in a container at a speed of 25r/min for 20min, and controlling the pH value to be 6.2-7.2; weighing the F group raw materials, stirring for 5min at a speed of 25r/min in a container to completely dissolve the powder into clear transparent liquid, and sterilizing and filtering by using a 0.22um filter for later use;
3) pumping the B group raw materials which are well heated and dissolved into an emulsifying pot by using an emulsifying pot vacuum pump, stirring at the speed of 25r/min, controlling the vacuum pressure at-0.03-0.05 MPa, slowly pumping the A group raw materials into the emulsifying pot, stirring at the speed of 30r/min, homogenizing at a high speed for 5min, and keeping the temperature of 80-83 ℃ for 15 min;
4) adding the pre-prepared group C raw materials into an emulsifying pot, stirring at a speed of 25r/min, cooling, and allowing the cooling water to flow at a speed of 0.5h/m 3 Vacuumizing to-0.03 MPa;
5) when the product in the step 4) is cooled to 48-50 ℃, weighing the raw materials in the group D, uniformly mixing, adding into an emulsifying pot, stirring at the speed of 25r/min, and cooling;
6) when the temperature of the product in the step 5) is reduced to below 45 ℃, respectively weighing the raw materials in the group E, adding the raw materials into an emulsifying pot, and stirring at the speed of 25r/min for 10min to obtain a semitransparent emulsion;
7) adding the prepared F group raw materials into an emulsifying pot, stirring at the speed of 25r/min, homogenizing at low speed for 1min to obtain semitransparent emulsion, and discharging after the inspection is qualified.
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