CN111110616A - Preparation method of tricholoma matsutake whitening mask liquid - Google Patents
Preparation method of tricholoma matsutake whitening mask liquid Download PDFInfo
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- CN111110616A CN111110616A CN202010054479.2A CN202010054479A CN111110616A CN 111110616 A CN111110616 A CN 111110616A CN 202010054479 A CN202010054479 A CN 202010054479A CN 111110616 A CN111110616 A CN 111110616A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9728—Fungi, e.g. yeasts
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/0212—Face masks
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/20—Chemical, physico-chemical or functional or structural properties of the composition as a whole
- A61K2800/30—Characterized by the absence of a particular group of ingredients
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
Abstract
The invention provides a preparation method of a tricholoma matsutake whitening mask liquid, which comprises the following steps: (1) physically crushing the tricholoma matsutake, mixing the crushed tricholoma matsutake with water, pulping to prepare tricholoma matsutake pretreatment liquid, crushing, drying and sieving the oat and the tremella to obtain oat powder and tremella powder; (2) adding the oat powder and the tremella powder obtained in the step (1) into the tricholoma matsutake pretreatment liquid obtained in the step (1), stirring and heating, and filtering to remove impurities; (3) and (3) adding vegetable oil into the solution obtained in the step (2), and homogenizing to obtain the facial mask liquid. The facial mask liquid has good moisturizing and whitening effects, the preparation method is simple in process, and the all-plant component tricholoma matsutake whitening facial mask prepared by the method has no irritation and high whitening effect.
Description
Technical Field
The invention belongs to the field of cosmetics, and particularly relates to a preparation method of tricholoma matsutake mask liquid.
Background
The conventional cosmetics are designed according to the principle of cosmetic formula, and on the basis of functional raw materials, thickening agents, emulsifying agents, preservatives, essences and the like are added for keeping the stability of appearance state and the commercialization requirement. In addition, in order to improve the whitening effect of the whitening cosmetics, various whitening functional components including whitening functional extracts derived from plants and the like and some whitening agents derived from non-plants are also added. For gestation, lactation and other people with sensitive skin, this increases the burden on the skin and even increases the risk of irritation and sensitivity.
As cosmetics pursue natural, whole plant ingredient market demands, more and more cosmetic formulations have reduced the use of non-plant derived materials. However, the existing whitening cosmetic technology and invention are only the compounding and post-processing of plant cosmetic raw materials, and are not controlled and changed from the source of the raw materials and the production technology, so that the requirements of market consumers on all plant components of facial mask liquid, nature and mild safety are met.
Disclosure of Invention
Therefore, the invention aims to overcome the defects in the prior art, control and change the sources of raw materials and the production technology, meet the requirements of market consumers on all plant components of the facial mask liquid, nature and safety, and provide the tricholoma matsutake whitening facial mask liquid, and the preparation method and the application thereof.
In order to achieve the above object, a first aspect of the present invention provides a preparation method of a tricholoma matsutake whitening mask liquid, including the following steps:
(1) physically crushing the tricholoma matsutake, mixing the crushed tricholoma matsutake with water, pulping to prepare tricholoma matsutake pretreatment liquid, crushing, drying and sieving the oat and the tremella to obtain oat powder and tremella powder;
(2) adding the oat powder and the tremella powder obtained in the step (1) into the tricholoma matsutake pretreatment liquid obtained in the step (1), stirring and heating, and filtering to remove impurities;
(3) and (3) adding vegetable oil into the solution obtained in the step (2), and homogenizing to obtain the facial mask liquid.
The preparation method according to the first aspect of the present invention, wherein, in the step (1), the preparation method of the tricholoma matsutake pretreatment liquid comprises the steps of: cutting Tricholoma matsutake into pieces, grinding with liquid nitrogen, mixing with water, and pulping.
According to the preparation method of the first aspect of the invention, in the step (1), the mass ratio of the tricholoma matsutake and the water in the tricholoma matsutake pretreatment liquid is 1: 10-1000, preferably 1: 50-600, more preferably 1: 100-400.
The preparation method of the first aspect of the invention comprises the steps of (1), sieving the oat flour by a sieve of 100-200 meshes, preferably 150 meshes; and/or
The tremella powder is screened by a 100-300-mesh sieve, and preferably a 200-mesh sieve.
According to the preparation method of the first aspect of the invention, in the step (2), the tricholoma matsutake pretreatment liquid, the oat flour and the tremella powder comprise the following components in parts by weight: 100 parts of tricholoma matsutake pretreatment liquid, 0.1-2 parts of oat powder and 0.1-10 parts of tremella powder;
preferably, 100 parts of tricholoma matsutake pretreatment liquid, 0.5-1 part of oat powder and 0.5-5 parts of tremella powder.
The preparation method according to the first aspect of the present invention, wherein, in the step (2), the stirring and heating temperature is 80 to 120 ℃, preferably 80 to 100 ℃, and most preferably 90 ℃; and/or
The stirring and heating time is 3-10 hours, preferably 4-6 hours, and most preferably 5 hours.
The production method according to the first aspect of the present invention, wherein, in the step (3), the vegetable oil is selected from one or more of: olive oil, prinsepia utilis royle oil, macadamia nut oil; preferably olive oil.
The preparation method according to the first aspect of the present invention, wherein in the step (3), the vegetable oil is added in an amount of 0.1 to 5%, preferably 0.5 to 2%, and most preferably 1% by mass of the solution obtained in the step (2).
The preparation method according to the first aspect of the present invention, wherein in the step (3), the homogenization speed is 1000 to 30000 r/min; and/or
The homogenizing time is 1-60 minutes, preferably 1-30 minutes, and more preferably 5-15 minutes.
In a second aspect of the invention, there is provided a facial mask comprising the facial mask fluid prepared according to the method of the first aspect.
The tricholoma matsutake whitening mask liquid disclosed by the invention has the following beneficial effects that:
1. the invention adopts medicinal and edible plants as raw materials (natural ingredients such as oat, tricholoma matsutake, tremella and olive oil) and water as a medium, and directly prepares the safe and mild full-plant whitening mask liquid with remarkable moisturizing and whitening effects by an extraction and impurity removal process, does not contain essence, preservative and bactericide, and does not generate stimulation and side effect while nourishing skin;
2. the facial mask liquid is absorbed by the facial mask carrier and applied on the facial skin, and the facial mask liquid has a skin care effect;
3. the facial mask liquid has good moisturizing and whitening effects, the preparation method is simple in process, and the all-plant component tricholoma matsutake whitening facial mask prepared by the method has no irritation and high whitening effect.
4. With the pursuit of cosmetics for natural and whole plant components, the market demand of the whole plant components is improved according to the particularity of plant raw materials, and the technology is more and more applied to the production of the whole plant cosmetics. The method for preparing the full-plant-component mask is applicable to preparation of mask solutions of various plant categories; no potential safety hazard exists; the efficacy is improved.
Drawings
FIG. 1 shows the effect of different concentrations of the tricholoma matsutake facial mask fluid of example 1 on HaCat cell viability in Experimental example 1.
FIG. 2 shows the effect of different concentrations of the tricholoma matsutake facial mask fluid of example 2 on HaCat cell viability in Experimental example 1.
FIG. 3 shows the effect of different concentrations of the tricholoma matsutake facial mask fluid of example 3 on HaCat cell viability in Experimental example 1.
FIG. 4 shows the effect of different concentrations of the tricholoma matsutake mask liquid of example 1 on the melanin content in B16 cells in Experimental example 3.
FIG. 5 shows the effect of different concentrations of the tricholoma matsutake mask liquid of example 2 on the melanin content in B16 cells in Experimental example 3.
FIG. 6 shows the effect of different concentrations of the tricholoma matsutake mask liquid of example 3 on the melanin content in B16 cells in Experimental example 3.
FIG. 7 shows the human skin moisture content test results of the tricholoma matsutake facial mask liquid of example 1 in test example 4.
FIG. 8 shows the human skin color test results of the tricholoma matsutake facial mask liquid of example 1 in test example 4.
Detailed Description
The invention is further illustrated by the following specific examples, which, however, are to be construed as merely illustrative, and not limitative of the remainder of the disclosure in any way whatsoever.
This section generally describes the materials used in the testing of the present invention, as well as the testing methods. Although many materials and methods of operation are known in the art for the purpose of carrying out the invention, the invention is nevertheless described herein in as detail as possible. It will be apparent to those skilled in the art that the materials and methods of operation used in the present invention are well within the skill of the art, provided that they are not specifically illustrated.
The reagents and instrumentation used in the following examples are as follows:
reagent:
oat (fried) was purchased from Zhangjiakou Jianjun oat food Co., Ltd;
matsutake is available from yunan yucheng long-warp corps;
tremella and olive oil are purchased from Yunan Yuancheng Longjing Daoji;
matsutake extracts (purchased) were purchased from guangzhou jiheng trade ltd;
tyrosinase, levodopa, purchased from sigma;
the instrument comprises the following steps:
homogenizer from IKA.
Example 1
This example illustrates the preparation of a plant mask solution according to the present invention.
(1) Sample pretreatment:
matsutake extracting solution: preserving fresh Tricholoma matsutake at low temperature, cutting into pieces, grinding with liquid nitrogen, mixing with water 1:
400, evenly mixing and pulping to prepare the tricholoma matsutake pretreatment liquid.
Oat: drying and crushing oat, sieving the oat powder with a 150-mesh sieve to obtain oat powder;
white fungus: drying and crushing tremella, sieving the tremella powder, and sieving the tremella powder with a 200-mesh sieve to obtain tremella powder;
(2) adding oat powder and tremella powder into the tricholoma matsutake pretreatment liquid obtained in the step (1) according to the mass ratio shown in the table, stirring and heating at 80 ℃ for 10 hours, and filtering to remove impurities.
| Classes of raw materials | Parts by mass |
| Tricholoma |
100 |
| Oat flour | 0.5 |
| |
5 |
(3) Adding 1% by mass of olive oil into the impurity-removed liquid obtained in the step (2), and homogenizing under the condition of 20000 rad/min; homogenizing for 5 min to obtain facial mask liquid.
Example 2
This example illustrates the preparation of a plant mask solution according to the present invention.
(1) Sample pretreatment:
matsutake extracting solution: preserving fresh Tricholoma matsutake at low temperature, cutting into pieces, grinding with liquid nitrogen, mixing with water 1:
100, evenly mixing and pulping to prepare the tricholoma matsutake pretreatment liquid.
Oat: drying and crushing oat, sieving the oat powder with a 150-mesh sieve to obtain oat powder;
white fungus: drying and crushing tremella, sieving the tremella powder, and sieving the tremella powder with a 200-mesh sieve to obtain tremella powder;
(2) adding oat powder/tremella powder into the tricholoma matsutake pretreatment liquid obtained in the step (1) according to the mass ratio shown in the following table, stirring and heating at 90 ℃ for 5 hours, and filtering by a 100-mesh sieve to remove impurities.
| Raw materialsClip for fixing | Parts by mass |
| Tricholoma |
100 |
| |
1 |
| Tremella powder | 0.5 |
(3) Adding 1% by mass of olive oil into the impurity-removed liquid obtained in the step (2), and homogenizing at 30000 rad/min; homogenizing for 15 min to obtain facial mask liquid.
Example 3
This example illustrates the preparation of a plant mask solution according to the present invention.
(4) Sample pretreatment:
matsutake extracting solution: preserving fresh Tricholoma matsutake at low temperature, cutting into pieces, grinding with liquid nitrogen, mixing with water 5:
100, evenly mixing and pulping to prepare the tricholoma matsutake pretreatment liquid.
Oat: drying and crushing oat, sieving the oat powder with a 200-mesh sieve to obtain oat powder;
white fungus: drying and crushing tremella, sieving the tremella powder, and sieving the tremella powder with a 200-mesh sieve to obtain tremella powder;
(5) adding oat powder/tremella powder into the tricholoma matsutake pretreatment liquid obtained in the step (1) according to the mass ratio shown in the following table, stirring and heating at 70 ℃ for 10 hours, and filtering by a 100-mesh sieve to remove impurities.
| Classes of raw materials | Parts by mass |
| Tricholoma |
100 |
| |
5 |
| Tremella powder | 0.5 |
Adding 5% by mass of olive oil into the impurity-removed liquid obtained in the step (2), and homogenizing under 10000 rad/min; homogenizing for 20 min to obtain facial mask liquid.
Test example 1
This example is provided to illustrate the results of HaCat cytotoxicity experiments on facial mask solutions prepared by the method of the present invention, and to illustrate the safety of the product.
Cells were collected in log phase and plated to a cell density of 5000 cells/well in 5% CO2Culturing at 37 deg.C, growing cells to a certain density, changing the culture solution, adding samples with different concentration gradients, and using the culture solution without sample as control. The incubation was continued for 24 hours, 20. mu.l of 0.5% MTT solution (i.e., 5mg/ml) was added to each well, the culture medium was discarded after 4 hours of incubation, carefully washed with PBS for 2-3 times, 150. mu.l of dimethyl sulfoxide was added to each well, and the mixture was shaken on a shaker at a low speed for 10min to dissolve the crystals sufficiently. The absorbance of each well was measured at 490 nm. Cell viability ═ (assay well OD value-blank OD value)/(cell control OD value-blank OD value) × 100%.
As shown in FIG. 1, the samples in FIG. 1 are the samples in example 1, the survival rate of HaCat cells is above 75% in the samples with concentration of 1% -5%, and the samples with concentration of 1.5% -2.5% have obvious proliferation promoting effect on HaCat cells, so that the cell survival rate is higher than 100%.
As shown in FIGS. 2 and 3, the samples in FIGS. 2 and 3 are the samples of examples 2 and 3, respectively, and the activity rates of the samples with the concentration of 1% -5% to HaCat cells are more than 75%, which proves that the detection samples have no cytotoxicity.
Test example 2
This test example is used to illustrate the anti-corrosion properties of the facial mask solution prepared by the method of the present invention.
After the sample is placed at room temperature of 25 ℃, low temperature of-20 ℃ and 60 ℃, the detection results of the microorganisms are shown in the following table 1:
TABLE 1 results of microbiological examination of products of examples 1-3
| Sample class | Standing at 25 deg.C for 3 years | Standing at 60 deg.C for 3 years | Standing at-20 deg.C for 3 years |
| Example 1 sample | Undetected for microorganisms | Undetected for microorganisms | Undetected for microorganisms |
| Example 2 sample | Undetected for microorganisms | Undetected for microorganisms | Undetected for microorganisms |
| Example 3 sample | Undetected for microorganisms | Undetected for microorganisms | Undetected for microorganisms |
Test example 3
This test example is intended to illustrate the experiments of inhibiting melanin synthesis in B16 cells by the use of the facial mask solutions of examples 1 to 3.
Counting B16 cells in log phase, inoculating to T25 flask, placing in incubator at 37 deg.C and 5% CO2Culturing overnight in the environment; changing the culture medium, adding samples (volume percentage) of examples 1, 2 and 3 with different concentrations, wherein a negative control group is not treated, a control group is 33mM arbutin (used for verifying the correctness of the experiment), sucking away the culture medium after culturing for 48 hours, adding lysis solution after washing by using PBS, scraping cells and collecting the cells in a centrifuge tube; water bath at 80 deg.C for 30 min; the mixture is shaken and mixed evenly, and then the mixture is absorbed to a 96-well plate to read the absorbance value at 475 nm. Melanin content change ═ (assay well OD value-blank OD value)/(cell control OD value-blank OD value). The results are shown in FIGS. 4-6.
As shown in the figure, FIGS. 4, 5 and 6 show the change of melanin content in melanocytes after the samples of examples 1, 2 and 3 are treated. It can be seen that the low level of 0.625% -2.5% treated samples had reduced melanin content.
Test example 4
This test example is intended to illustrate the results of human testing of the facial mask solution in example 1.
Based on the full-face design, 30 eligible female subjects participated in the test with a test period of 4 weeks. During the test period, the test product is used by the test subject on the whole face once a day at night for 4 weeks, the original cleaning and skin care mode, habit and brand are kept unchanged by the test subject, only the original facial mask is stopped (if the original facial mask is used), and the procedure of using the test facial mask is added. Before (initial value), after 2 and 4 weeks of use of the test product, the skin moisture content test, skin color test, skin gloss test, skin elasticity test, skin texture and roughness test were performed on one side of the cheek at random, and the whole face was subjected to imaging test (left, middle, right 3 sides) and questionnaire.
(1) Skin moisture content test results
The skin moisture content test was performed on one of the randomized cheeks before, 2 weeks, and 4 weeks after use of the test product, and the test data for the subject was calculated as shown in fig. 7.
As can be seen from fig. 7, the skin moisture content in the test area was significantly increased compared to the initial value at both 2 and 4 weeks after use of the test product. A larger value of the parameter of skin moisture content indicates a higher moisture content of the stratum corneum of the skin.
(2) Skin color test results
Skin color test was performed on one of the randomized cheeks before, 2 weeks, and 4 weeks after use of the test product, and the test data for the subject was calculated as shown in fig. 8. As can be seen from fig. 8, both the L and b values of the test area were significantly increased compared to the initial values at 2 and 4 weeks after use of the test product. The ITA ° values tended to increase but no significant change was seen. A larger value of L x of the skin color parameter indicates a whiter skin color, a larger value of b x indicates a yellowish skin color, and a larger value of ITA ° (individual type angle) indicates a brighter skin.
Although the present invention has been described to a certain extent, it is apparent that appropriate changes in the respective conditions may be made without departing from the spirit and scope of the present invention. It is to be understood that the invention is not limited to the described embodiments, but is to be accorded the scope consistent with the claims, including equivalents of each element described.
Claims (10)
1. The preparation method of the tricholoma matsutake whitening mask liquid is characterized by comprising the following steps:
(1) physically crushing the tricholoma matsutake, mixing the crushed tricholoma matsutake with water, pulping to prepare tricholoma matsutake pretreatment liquid, crushing, drying and sieving the oat and the tremella to obtain oat powder and tremella powder;
(2) adding the oat powder and the tremella powder obtained in the step (1) into the tricholoma matsutake pretreatment liquid obtained in the step (1), stirring and heating, and filtering to remove impurities;
(3) and (3) adding vegetable oil into the solution obtained in the step (2), and homogenizing to obtain the facial mask liquid.
2. The preparation method according to claim 1, wherein the tricholoma matsutake pretreatment liquid is prepared by the method comprising the steps of: cutting Tricholoma matsutake into pieces, grinding with liquid nitrogen, mixing with water, and pulping.
3. The preparation method according to claim 1 or 2, wherein in the step (1), the mass ratio of the tricholoma matsutake and the water in the tricholoma matsutake pretreatment liquid is 1: 10-1000, preferably 1: 50-600, more preferably 1: 100-400.
4. The preparation method according to any one of claims 1 to 3, wherein in the step (1), the oat flour is sieved by a sieve with 100-200 meshes, preferably 150 meshes; and/or
The tremella powder is screened by a 100-300-mesh sieve, and preferably a 200-mesh sieve.
5. The preparation method according to any one of claims 1 to 4, wherein in the step (2), the tricholoma matsutake pretreatment liquid, the oat flour and the tremella powder are prepared from the following components in parts by weight: 100 parts of tricholoma matsutake pretreatment liquid, 0.1-2 parts of oat powder and 0.1-10 parts of tremella powder;
preferably, 100 parts of tricholoma matsutake pretreatment liquid, 0.5-1 part of oat powder and 0.5-5 parts of tremella powder.
6. The method according to any one of claims 1 to 5, wherein in the step (2), the stirring and heating temperature is 80 to 120 ℃, preferably 80 to 100 ℃, and most preferably 90 ℃; and/or
The stirring and heating time is 3-10 hours, preferably 4-6 hours, and most preferably 5 hours.
7. The method according to any one of claims 1 to 6, wherein in the step (3), the vegetable oil is selected from one or more of: olive oil, prinsepia utilis royle oil, macadamia nut oil; preferably olive oil.
8. The method according to any one of claims 1 to 7, wherein the vegetable oil is added in the step (3) in an amount of 0.1 to 5%, preferably 0.5 to 2%, and most preferably 1% by mass based on the solution obtained in the step (2).
9. The production method according to any one of claims 1 to 8, wherein in the step (3), the homogenization speed is 1000 to 30000 r/min; and/or
The homogenizing time is 1-60 minutes, preferably 1-30 minutes, and more preferably 5-15 minutes.
10. A facial mask, characterized in that it comprises a facial mask solution prepared according to the method of any one of claims 1 to 9.
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| CN113230166A (en) * | 2021-04-16 | 2021-08-10 | 云南白药集团股份有限公司 | Mask liquid, preparation method and application thereof |
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