Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to specific embodiments. It should be understood that the detailed description and specific examples, while indicating the invention, are intended for purposes of illustration only and are not intended to limit the scope of the invention.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
The embodiment of the invention provides a moisturizing cosmetic composition which comprises the following raw material components in percentage by mass:
70 to 99 percent of birch juice,
0.1 to 5 percent of nicotinamide,
0.1 to 10 percent of dictyophora indusiata extract,
And the balance auxiliary ingredients, which do not contain water added as a separate component.
It is to be noted that the absence of water added as a separate component of the auxiliary ingredient means that the auxiliary ingredient does not contain any added water, but does not exclude small or trace amounts of water that are inevitably introduced in the components of the auxiliary ingredient, such as: moisture retention enhancers, thickeners or bacteriostats as auxiliary components contain trace amounts of water.
The birch juice is used as base material, is extracted from natural birch, and has rich nutrient substances. In addition, birch sap contains natural minerals such as: calcium, magnesium and potassium ions are beneficial to regulating the microenvironment for transmembrane transport of moisture, so that moisture and active ingredients (such as nicotinamide and dictyophora phalloidea extract) in the moisturizing cosmetic composition are transported into the horny layer.
Collecting birch juice by drilling hole on birch trunk, draining in drainage device, filtering, and sterilizing by conventional method to obtain colorless transparent juice with no precipitate and impurities and rich nutrients. The collected birch juice is not subjected to irradiation treatment and added with preservative, so that the nutrient components of the birch juice are reserved to the maximum extent. The birch juice is commercially available and used as is, for example, from greater Khingan than wild berry development, Inc.
Preferably, the birch sap is birch sap. The birch juice is selected to have the advantages of abundant nutrition and good microenvironment for regulating water transport across membranes, so as to transport water and active ingredients (such as nicotinamide and Dictyophora Indusiata extract) in the moisturizing cosmetic composition into stratum corneum.
Nicotinamide, also known as vitamin B3(VB3), is a bioactive form of niacin and is widely found in various animals and plants. Wherein, the main functions of the nicotinamide in the invention are moisturizing, anti-wrinkle and whitening effects. For whitening efficacy, niacinamide down regulates the transport of melanosomes from melanocytes to keratinocytes without inhibiting tyrosinase activity or cell proliferation, thereby affecting skin pigmentation, and it interferes with the cell signaling pathway between keratinocytes and melanocytes, reducing melanogenesis. On the other hand, niacinamide acts on melanin already produced, reducing its transfer to the superficial cells. For moisturizing effect, niacinamide can increase the levels of free fatty acids and ceramides in the skin, stimulate microcirculation in the dermal layer, and prevent water loss from the skin. It also enhances protein synthesis (e.g., keratin), increases intracellular NADPH levels, and accelerates differentiation of keratin cells. That is, niacinamide can improve the skin barrier function and has moisturizing ability, so that the niacinamide is cooperated with birch juice to jointly regulate the microenvironment of the skin surface, facilitate moisture transportation and play a role in moisturizing.
Dictyophora indusiata is a rare edible fungus, is rich in various high-quality plant nutrient proteins, has high protein content and contains a whole set of amino acids for forming proteins, wherein the content of essential amino acids accounts for more than 1/3 of total amino acids, exceeds grains and some meats, and the content of glutamic acid is most abundant and accounts for more than 17.0 percent of the total amount of the amino acids. The dictyophora indusiata fruiting body is crisp and delicious, has aromatic flavor and delicious taste, and is known as the "queen in fungus" and the "king of mountain delicacies". Dictyophora indusiata belongs to physiological alkaline food, can regulate the content of ascorbic acid and fatty acid in human body, and has obvious effect of lowering blood pressure, and can also lower cholesterol in vivo and reduce fat storage in abdominal wall. The high-activity macromolecular substance polysaccharide component contained in the bamboo fungus has the curative effects of inhibiting tumor, resisting blood coagulation, resisting inflammation, stimulating immunity, reducing blood sugar and the like, and has good effects of whitening and moisturizing skin.
The bamboo fungus extract liquid contains a large amount of high molecular polysaccharides, can improve nutrition of skin surface layers, and further has the effects of whitening and moisturizing. The dictyophora indusiata extract is a commercial product and is purchased from vast Sen biotechnology limited of Beijing east.
The sources of the raw materials used in the bamboo shoot extract in the invention are not limited too much, and can be dictyophora indusiata, dictyophora phalloidea or dictyophora echinovolvata and the like.
In one particular example, the adjunct ingredients include one or more of a moisturizing enhancer, a thickening agent, and a bacteriostatic agent.
The moisturizing enhancer refers to a moisturizing agent that further enhances the moisturizing effect of the moisturizing cosmetic composition.
In a specific example, the moisturizing enhancer is selected from one or more of butylene glycol, sodium hyaluronate, propylene glycol, and tremella extract. Preferably, the moisturizing enhancer is selected from the combination of butanediol, sodium hyaluronate and tremella extract, so that the moisturizing effect of the moisturizing cosmetic composition can be enhanced better.
In a specific example, the moisturizing enhancer contains sodium hyaluronate, and the sodium hyaluronate accounts for 0.01-0.5% of the moisturizing cosmetic composition by mass, and preferably accounts for 0.01-0.05% of the moisturizing cosmetic composition by mass. More preferably, the sodium hyaluronate is enzyme-hydrolyzed sodium hyaluronate after enzyme digestion treatment. The enzymolysis sodium hyaluronate is a molecular fragment with extremely small molecular weight, which is prepared by the enzyme digestion of sodium hyaluronate and has the molecular weight of 3-4 KDa. The enzymatic sodium hyaluronate can quickly permeate below the horny layer, and provides a lasting skin moisturizing effect. The enzymatically hydrolyzed sodium hyaluronate can be prepared by a method known in the art, for example, by first preparing sodium hyaluronate from a culture medium of vegetal origin (peptone, glucose, etc.) by a fermentation method, and then preparing hydrolyzed sodium hyaluronate by an enzymatic cleavage. Wherein, the enzyme digestion method is selected from the test methods generally adopted by the technicians in the field, and the details are not repeated herein. The enzymolysis sodium hyaluronate adopted by the invention has the characteristics of small molecular weight and rapid penetration, and can directly reach the dermis layer so as to achieve the effects of deep water locking, water replenishing, cell proliferation promotion and collagen generation stimulation. While ordinary hyaluronic acid can be used in an acidic formulation, it is easily combined with cations in the formulation and cannot exist stably.
In a specific example, the moisturizing enhancer contains butanediol, and the mass percentage of the butanediol in the moisturizing cosmetic composition is 0.1% -20%. Preferably, the butanediol accounts for 8% -12% of the moisturizing cosmetic composition by mass.
The thickener can bind other components in the moisturizing cosmetic composition together to enhance the rheological plasticity of the cosmetic composition.
In a specific example, the thickening agent is gum arabic, and the thickening effect is better. It is understood that the thickener in this embodiment may also be xanthan gum, carrageenan, seaweed gel, and other conventional thickeners.
In one particular example, the bacteriostatic agent may enhance the bacteriostatic activity of the moisturizing cosmetic composition, enhancing the extended period of the moisturizing cosmetic. The preservative in the present invention may be one generally selected by those skilled in the art, such as natural preservatives and chemically synthesized preservatives. Preferably, the bacteriostatic agent is caprylyl hydroximic acid which is an ideal organic acid, has excellent antibacterial and bacteriostatic properties, and can be used for replacing a traditional preservative formula system.
In one specific example, the moisturizing enhancer contains a tremella extract which is rich in mucopolysaccharides such as glucose, trehalose, pentose polyacid, mannitol and the like, and can be used for enhancing the moisturizing performance of the moisturizing cosmetic composition and improving the smooth feeling of the skin.
In a specific example, the tremella extract accounts for 0.1-15% of the moisturizing cosmetic composition by mass. Preferably, the tremella extract accounts for 1-8% of the moisturizing cosmetic composition by mass.
In a specific example, the moisturizing cosmetic composition comprises the following raw material components in percentage by mass:
in a specific example, the moisturizing cosmetic composition comprises the following raw material components in percentage by mass:
in a specific example, the moisturizing cosmetic composition comprises the following raw material components in percentage by mass:
the moisturizing cosmetic composition is prepared by mixing birch juice, nicotinamide and dictyophora phalloidea extract, and has reasonable proportion, and the obtained cosmetic composition has good moisturizing and whitening effects. Wherein, the birch juice is used as a base material, has rich nutrient substances and natural mineral substances, and is beneficial to transporting water to the horny layer through a membrane, thereby improving the water content of the skin and playing a role in moisturizing. Under the environment rich in mineral ions provided by the birch juice, nutrient components in the nicotinamide and the dictyophora phalloidea extract are transported to the cuticle in a transmembrane transport mode, and the nutrient components have the effects of moisturizing and whitening. In addition, niacinamide can also improve the skin barrier function, so that the niacinamide is cooperated with birch juice to jointly regulate the microenvironment on the surface of the skin, facilitate moisture transportation and play a role in moisturizing.
The invention also provides a preparation method of the moisturizing cosmetic composition, which comprises the following steps:
and S1, weighing the raw material components according to the corresponding weight percentage.
S2, mixing the raw material components uniformly to obtain a mixture.
In one specific example, gum arabic and sodium hyaluronate are added to butanediol and heated with stirring to 68 ℃ to 75 ℃ to obtain a premix; compared with a mode of directly mixing the raw material components, the method has the advantage that the acacia gum and the sodium hyaluronate are added into the butanediol under the heating condition, so that the agglomeration phenomenon can be avoided. Then mixing the birch juice with the premix, and uniformly stirring for the second time to fully mix the birch juice and the premix; adding nicotinamide, tremella extract and bamboo fungus extract into the premix obtained after the second stirring and mixing, and stirring and mixing for the third time to fully mix the three; and finally, adding the caprylyl hydroximic acid, and uniformly stirring for the fourth time to fully mix the final product to obtain a mixture.
Wherein, the device for stirring and mixing is selected from a conventional mixing device without special limitation.
In one specific example, the second stirring is carried out at a frequency of 50 to 70 rpm for 18 to 22 minutes.
S3, filtering the mixture with a filter cloth to obtain the moisturizing cosmetic composition.
Wherein, the filter cloth has no special requirement, and can be made of gauze commonly used in the cosmetic field.
In a specific example, the filter cloth has a mesh number of 280-320 meshes. And further, large particles of raw materials which are not adhered together in the moisturizing cosmetic composition can be removed better. The preparation method is simple, and the moisturizing cosmetic composition can be obtained only by stirring and uniformly mixing. The moisturizing cosmetic composition prepared by the preparation method of the moisturizing cosmetic composition has the moisturizing and whitening effects.
One embodiment of the present invention provides a toner, a mask or an emulsion, which contains the moisturizing cosmetic composition according to the present invention.
The toner, the mask or the emulsion can be prepared by adopting a method commonly used by technicians in the field of cosmetics.
The toner, the facial mask or the emulsion has good moisturizing effect.
The present invention will be further described with reference to the following specific examples.
Example 1
A method for preparing a moisturizing cosmetic composition comprises the following steps:
the raw material components are measured according to the weight percentage in the table 1,
TABLE 1
Components
|
Percentage content (%)
|
Birch sap
|
87
|
Nicotinamide
|
3
|
Dictyophora indusiata extract
|
7.5
|
Hyaluronic acid sodium salt
|
2.5 |
Then placing the raw material components in a mixing pot, mixing uniformly to obtain a mixture, and filtering the mixture by using 300-mesh gauze to obtain the moisturizing cosmetic composition.
Example 2
A method for preparing a moisturizing cosmetic composition comprises the following steps:
the raw material components are measured according to the weight percentage in the table 2,
TABLE 2
Components
|
Percentage content (%)
|
Birch sap
|
71
|
Nicotinamide
|
4
|
Dictyophora indusiata extract
|
3
|
Hyaluronic acid sodium salt
|
0.02
|
Butanediol
|
13.28
|
Tremella extract
|
8
|
Arabic gum
|
0.2
|
Octanoyl hydroximic acid
|
0.4 |
Adding gum arabic and sodium hyaluronate into butanediol, heating and stirring in a mixing pot to 70 ℃ to obtain a premix, mixing birch juice with the premix, stirring for the second time at a speed of 60 rpm for 20min, adding nicotinamide, tremella extract and bamboo fungus extract into the premix obtained after stirring and mixing for the second time, stirring for the third time for 10min, and finally adding caprylyl hydroximic acid and stirring for the fourth time for 10min to obtain a mixture.
Filtering the mixture with 300 mesh gauze to obtain the moisturizing cosmetic composition.
Example 3
A method for preparing a moisturizing cosmetic composition comprises the following steps:
the raw material components were measured according to the weight percentages in table 3,
TABLE 3
Adding gum arabic and enzymolysis sodium hyaluronate into butanediol, heating and stirring in a mixing pot to 70 ℃ to obtain a premix, mixing birch juice with the premix, stirring for the second time at a speed of 60 revolutions per minute for 20min, adding nicotinamide, tremella extract and bamboo fungus extract into the premix obtained by stirring and mixing for the second time, stirring for the third time for 10min, and finally adding octanoyl hydroximic acid and stirring for the fourth time for 10min to obtain a mixture.
Filtering the mixture with 300 mesh gauze to obtain the moisturizing cosmetic composition.
Comparative example 1
A method for preparing a moisturizing cosmetic composition comprises the following steps:
the raw material components were measured according to the weight percentages in table 4,
TABLE 4
Components
|
Percentage content (%)
|
Birch sap
|
60
|
Nicotinamide
|
15
|
Dictyophora indusiata extract
|
22.5
|
Hyaluronic acid sodium salt
|
2.5 |
Then placing the raw material components in a mixing pot, mixing uniformly to obtain a mixture, and filtering the mixture by using 300-mesh gauze to obtain the moisturizing cosmetic composition.
Comparative example 2
A method for preparing a moisturizing cosmetic composition comprises the following steps:
the raw material components were measured in the weight percentages in table 5,
TABLE 5
Then placing the raw material components in a mixing pot, mixing uniformly to obtain a mixture, and filtering the mixture by using 300-mesh gauze to obtain the moisturizing cosmetic composition.
Comparative example 3
A method for preparing a moisturizing cosmetic composition comprises the following steps:
the raw material components were measured in the weight percentages in table 6,
TABLE 6
Components
|
Percentage content (%)
|
Birch sap
|
97.5
|
Nicotinamide
|
2.5
|
Dictyophora indusiata extract
|
0
|
Hyaluronic acid sodium salt
|
0 |
Then placing the raw material components in a mixing pot, mixing uniformly to obtain a mixture, and filtering the mixture by using 300-mesh gauze to obtain the moisturizing cosmetic composition.
Comparative example 4
A method for preparing a moisturizing cosmetic composition comprises the following steps:
the raw material components were measured in the weight percentages in table 7,
TABLE 7
Components
|
Percentage content (%)
|
Birch sap
|
79.8
|
Water (W)
|
20
|
Hyaluronic acid
|
0.2 |
Then placing the raw material components in a mixing pot, mixing uniformly to obtain a mixture, and filtering the mixture by using 300-mesh gauze to obtain the moisturizing cosmetic composition.
And (3) performance testing:
product safety assessment
The evaluation method comprises the following steps:
the test substance: the moisturizing cosmetic compositions of examples 1 to 3 and comparative examples 1 to 4 of the present invention.
Negative control: blank control.
Subject: for women of 18-40 years old, the eligible subjects were divided into 7 groups on average, and 30 subjects in each group corresponded to the groups of examples 1-3 and comparative examples 1-4 of the present invention.
The spot test method comprises the following steps: selecting a qualified spot tester, applying about 0.02-0.025 ml of a test object into the spot tester by a closed spot test method, externally applying a special adhesive tape to the curved side of the forearm of a subject, removing the test object after 24 hours, observing skin reaction 30min after the spot tester of the test object is removed, and recording the result according to the skin reaction grading standard in the cosmetic hygiene Specification (2007 edition).
And (4) evaluating the results: the results were recorded as in Table 8 (grading Standard for adverse skin reactions).
TABLE 8
1) And (3) test results:
TABLE 9
The result of the human body skin patch test shows that:
in all examples of the present invention, the skin of the subject was all negatively responsive (score scale: 0). According to the judgment standard of a human skin patch test in the cosmetic hygiene Specification (2007 edition), the sample has no adverse skin reaction on the human body. In comparative example 2 (not containing a certain ingredient of the cosmetic moisturizing composition of the present invention), 2 subjects showed grade 1 reactions.
Evaluation of moisturizing Effect
Test samples: the moisturizing cosmetic compositions of examples 1 to 3 and comparative examples 1 to 4 of the present invention.
Subject: for women of 25 to 44 years old, the eligible subjects were divided into 7 groups on average, and 30 subjects in each group corresponded to the groups of examples 1 to 3 of the present invention and the groups of comparative examples 1 to 4.
The tested part: marking three tested parts on the inner sides of the two forearms, and randomly distributing the tested parts into a sample painting area 1, a sample painting area 2 and a sample painting area 3, wherein the sample painting area is used for painting the tested objects.
The smearing area of the test object is 3cm multiplied by 3cm, and the smearing dosage is as follows: 2mg/cm2。
The testing steps are as follows: see QB/T4256 + 2011 guide for evaluation of moisturizing efficacy of cosmetics.
And (3) testing results: the results of the sample application zone tests are shown in Table 10.
Watch 10
Note: the stratum corneum moisture content of the skin was measured before application (T0) to the test area.
The application area 1 is the water content of stratum corneum of the skin 1 hour after application of the test substance (T1).
The application area 2 is the water content of the stratum corneum of the skin 4 hours after application of the test substance (T2).
The application area 3 is the water content of the stratum corneum of the skin 8 hours after the application of the test substance (T3).
The subjects in each of the examples had higher water content in the stratum corneum than in the comparative examples under the test conditions of 1 hour after application, 4 hours after application, and 8 hours after application. Further, 1 hour after application, there was a significant difference in the moisture content of example 3 as compared with comparative example 1 (the raw material components were not in the compounding ratio range of the cosmetic moisturizing composition of the present invention), comparative example 2 (not containing a certain component of the cosmetic moisturizing composition of the present invention), and comparative example 3 (not containing a certain component of the cosmetic moisturizing composition of the present invention). At 4 hours after application, there was a significant difference in the moisture content of example 3 as compared with comparative example 1 (the raw material components were not in the compounding ratio range of the cosmetic moisturizing composition of the present invention), comparative example 2 (not containing a certain component of the cosmetic moisturizing composition of the present invention), and comparative example 3 (not containing a certain component of the cosmetic moisturizing composition of the present invention). At 8 hours after application, there was a significant difference in the moisture content of example 3 as compared with comparative example 1 (the raw material components were not in the compounding ratio range of the cosmetic moisturizing composition of the present invention), comparative example 2 (not containing a certain component of the cosmetic moisturizing composition of the present invention), and comparative example 3 (not containing a certain component of the cosmetic moisturizing composition of the present invention). The above results show that the moisturizing cosmetic composition of the present invention has a good moisturizing effect on skin.
Evaluation of whitening Effect
Test samples: the moisturizing cosmetic compositions of examples 1 to 3 and comparative examples 1 to 4 of the present invention.
Subject: women of 25-44 years old have dark and lusterless facial complexion.
The testing steps are as follows: the eligible subjects were divided into 7 groups on average, and 30 subjects in each group corresponded to the groups of examples 1 to 3 of the present invention and the groups of comparative examples 1 to 4. After each subject cleans the face, the moisture-keeping cosmetic composition in the corresponding group is evenly smeared at the position of 3cm multiplied by 3cm of the cheek with 2mg/cm2The whitening effect was evaluated 2 times a Day, once in the morning and evening, after four consecutive weeks of use (Day28) and after eight weeks of use (Day 56).
The evaluation method comprises the following steps: the evaluation of the whitening effect of the subjects on the improvement of the skin color after the product was used was known on the 28 th and 56 th days by means of questionnaire. The evaluation indexes are as follows: the whitening effect is not obvious, the whitening effect is general, the whitening effect is obvious, and the whitening effect is very obvious.
1. After four weeks of use (Day28)
TABLE 11
2. Eight weeks later (Day56)
TABLE 12
As can be seen from table 11, in the results of the whitening effect evaluation of each example group after 4 weeks, more than 60% of the subjects felt a relatively significant whitening effect, while more than 90% of the subjects felt a relatively significant whitening effect in example 3. In comparative example 1 (the proportion of the raw material components is not within the range of the proportion of the moisturizing composition of the cosmetic), only 5 subjects with obvious effect exist, and the moisturizing composition of the cosmetic is proved to be lack of success and reasonable in composition. The whitening effects of comparative example 2 (not containing a component of the cosmetic moisturizing composition of the present invention) and comparative example 3 (not containing a component of the cosmetic moisturizing composition of the present invention) were also poor, indicating that the moisturizing composition of the present invention is disadvantageous. Also, the whitening effect trend in table 12 is similar to that in table 11 and will not be described again. In addition, as can be seen from the groups of examples in table 12, the whitening effect becomes better and better when the cosmetic moisturizing composition of the present invention is used for a long time.
The features of the above-described embodiments may be arbitrarily combined, and for the sake of brevity, all possible combinations of the features in the above-described embodiments are not described, but should be construed as being within the scope of the present disclosure as long as there is no contradiction between the combinations of the features.
The above embodiments only express a few embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.