CN114569536A

CN114569536A - A lotion for improving microecology and physiological state of aged skin

Info

Publication number: CN114569536A
Application number: CN202210196689.4A
Authority: CN
Inventors: 李燕; 韩婷婷; 姜姗姗; 陈玉荣; 王兴凯; 杨素珍; 王彩霞
Original assignee: Shandong Furida Biological Co ltd
Current assignee: Shandong Furida Biological Co ltd
Priority date: 2022-03-01
Filing date: 2022-03-01
Publication date: 2022-06-03
Anticipated expiration: 2042-03-01
Also published as: CN114569536B

Abstract

The invention discloses an emulsion for improving the skin micro-ecology and the skin physiological state of aging, which takes brown algae yeast fermentation lysate, lactobacillus/pear juice fermentation filtrate and short-chain fatty acid salt as main effective components, can obviously improve the skin micro-ecology of aging people through crowd test verification, and has the effect of improving the skin physiological state of the aging people. Meanwhile, the emulsion can enhance the barrier of aging skin, promote the synthesis of elastin, enable the product to have the efficacy of anti-wrinkle repair, and can be added into an anti-aging skin care product which is friendly to skin micro-ecology.

Description

A lotion for improving microecology and physiological state of aged skin

Technical Field

The invention relates to an emulsion for improving the microecology and physiological state of aged skin, belonging to the technical field of skin care products.

Background

With the increasing aging of the global population, anti-aging is a constant subject for human beings. Research on skin aging and skin rejuvenation is also becoming a research hotspot. The skin is the largest organ of the human body in direct contact with the external environment, and is the first defense line for protecting the body from loss of nutrients and preventing invasion of external harmful factors. The barrier function of skin is divided into physical, chemical and biological barriers. The biological barrier is used for preventing the colonization of pathogenic microbial flora and temporary flora and maintaining the steady state of resident flora. The skin microbial flora has important significance for the health of organisms, particularly the health of skin. The skin microbial flora not only helps the organism clean, metabolize skin secretion, excretion and metabolic substances, but also converts the substances with nutritive value to the skin to be absorbed and utilized by the skin again, and can also regulate the innate and adaptive immune systems of the organism. With the increase of age, i.e. the development of the natural aging process, the microbial flora of the skin changes obviously.

Skin microorganisms are mainly present on the skin surface and in skin appendages such as hair follicles, sweat glands, sebaceous glands, etc., of which the most representative microorganisms include the genera propionibacterium, corynebacterium, staphylococcus, and malassezia. Multiple studies show that the ratio of firmicutes, actinomycetes, proteobacteria and bacteroidetes at different ages is different at phylum level with the increase of age; in particular, the proportion of the proteobacteria of the senior group is reduced from 35% to 21.9% relative to that of the adult group. In the study published on the nature in 2017, the flora conditions at different sites (cheek, forearm, forehead and scalp) were compared between the young and the elderly group, and an analysis based on the observed number of OTUs showed that the species abundance was significantly higher in the elderly group than in the young group at all test sites, and that in all skin microbiome the elderly group showed a higher trend of alpha diversity than in the young group. At these sites, corynebacterium showed higher abundance in the elderly cheek and forehead microorganisms; propionibacterium showed higher abundance in the cheeks and forehead of the young group; staphylococcus showed higher abundance in the forearms of the young group; streptococci and prevotella showed higher abundance in the young group. In another study, age groups of 20-30s, 50-60s had significant differences at the phylum level, with bacteroidetes and firmicutes having higher abundances in 20-30s and proteobacteria and actinomycetes having higher abundances in 50-60 s. More and more studies have shown that with age, the skin's micro-ecology changes constantly, interacting with the state of the skin. In the latest study, the abundances of 20-29s, 40-53s facial skin microbiome flora in korean healthy women were compared, streptococcus was the most different in relative abundances of two age groups, and in combination with the difference in skin elasticity index between the two age groups, streptococcus was positively correlated with skin elasticity.

The microecology of aging skin is greatly altered compared to young skin, and also affects the physiological state of the skin, including parameters such as skin elasticity, firmness, and skin sensitivity. CN20210104295.7 discloses a composition beneficial to skin micro-ecology after staying up, a preparation method and application thereof, which illustrate the change of skin flora after staying up and obtain an innovative composition beneficial to skin micro-ecology after staying up from improving the flora balance of skin micro-ecology after staying up. However, no method for making the skin micro-ecology of the aged skin more similar to the micro-ecology of the young skin has been reported, and accordingly, there has been no research on the raw materials or the components for improving the micro-flora of the aged skin and the verification of the improvement of the micro-ecology of the aged skin.

Disclosure of Invention

The invention aims to provide an emulsion which has an effect of improving the microecology of aged skin and the physiological state of the skin. The emulsion contains brown algae yeast fermentation lysate, lactobacillus/pear juice fermentation filtrate, and short chain fatty acid salt as main effective components, and can improve microecology of aged skin and physiological state of skin. Meanwhile, the emulsion can enhance the barrier of aging skin, promote the synthesis of elastin and enable the product to have the effects of anti-wrinkle repair.

The technical scheme of the invention is as follows: an emulsion for improving the microecology and physiological state of aged skin, which is characterized by comprising three effective components of phaeophyceae yeast fermentation lysate, lactobacillus/pear juice fermentation filtrate and short-chain fatty acid salt.

The dosage of the three effective components in the emulsion is as follows: each 20g of the emulsion contains 0.1-0.3g of fucoidan yeast fermentation lysate (calculated by dry matter, the same below), 0.5-8.0g of lactobacillus/pear juice fermentation filtrate, and 0.05-0.1g of short-chain fatty acid salt. Preferably: each 20g of the emulsion contains 0.2g of the phaeophyceae yeast fermentation lysate, 3.0g of lactobacillus/pear juice fermentation filtrate and 0.06g of short-chain fatty acid salt.

The preparation method of the brown algae yeast fermentation lysate comprises the following steps: inoculating yeast into a brown algae aqueous solution for fermentation, breaking the walls of fermentation liquor by a high-pressure homogenizer, crushing thalli, and filtering to obtain the brown algae liquid, wherein the mass ratio of brown algae (wet) to water is 1: 2-4, and preferably 1: 3; the yeast is Saccharomyces cerevisiae or cerevisiae Fermentum, preferably Saccharomyces cerevisiae; the fermentation temperature is 30-38 deg.C, preferably 36 deg.C; the fermentation time is 48-96h, preferably 72 h; the pore size of the fermentation liquid filter is 200 meshes and 400 meshes, preferably 400 meshes.

The preparation method of the lactobacillus/pear juice fermentation filtrate comprises the following steps: after lactobacillus is fermented and cultured in a pear juice culture medium (20 +/-5 percent of freshly squeezed pear juice is added into an MRS culture medium), centrifugal separation is carried out, and supernate is taken, namely lactobacillus/pear juice fermentation filtrate, wherein the main components of the lactobacillus/pear juice fermentation filtrate are lactic acid, amino acid and the like, and the content of the lactic acid is 2.0-6.0 percent (wt percent). The fermentation temperature is 30-38 deg.C, preferably 37 deg.C; the fermentation time is 24-72h, preferably 48 h.

Furthermore, the invention can also be added with sucrose ester emulsifier, natural grease, caprylic/capric triglyceride, polyalcohol and water to prepare an optimal emulsion formula, and the components and the dosage are as follows: every 20g of the emulsion contains 0.1-0.3g of phaeophyceae fermentation lysate, 0.5-8.0g of lactobacillus/pear juice fermentation filtrate, 0.05-0.1g of short-chain fatty acid salt, 0.2-2.0g of sucrose ester emulsifier, 0.5-10.0g of natural grease, 1.0-4.0g of caprylic/capric triglyceride, 0.5-10.0g of polyhydric alcohol and the balance of water.

Further preferably, the components and the using amount are as follows: every 20g of the phaeophyceae fermentation lysate is 0.2g, the lactobacillus/pear juice fermentation filtrate is 3.0g, the short-chain fatty acid salt is 0.06g, the sucrose ester emulsifier is 1.0g, the natural oil is 4.0g, the caprylic/capric triglyceride is 2.5g, the polyalcohol is 5.5g and the balance of water.

The short-chain fatty acid salt is one or two of lactate, butyrate, propionate and isobutyrate, and is preferably calcium propionate.

The sucrose lipid emulsifier is one or two of sucrose laurate, sucrose palmitate and sucrose stearate, preferably sucrose laurate.

The natural oil is one or two of white Potentilla seed oil, Malula oil, oleum Camelliae Japonicae, oleum Cocois, and bitter almond oil.

The polyhydric alcohol is one or two of caprylyl glycol, pentanediol, butanediol, glycerol, dipropylene glycol and hexanediol.

The preparation method of the emulsion is characterized by comprising the following steps:

s1: mixing sucrose ester emulsifier and polyhydric alcohol, heating for dissolving, adding water, and cooling to form a liquid I;

s2: mixing natural oil and caprylic/capric triglyceride to form a liquid II;

s3: adding the phaeophyceae yeast fermentation lysate, short-chain fatty acid salt and lactobacillus/pear juice fermentation liquor into the mixed liquor of the first liquid, and stirring and mixing uniformly to form a third liquid;

s4: slowly adding the second liquid into the third liquid, and homogenizing at high speed to form a uniform emulsion. The high-speed homogenizing rotation speed is 8000-10000r/min, and the homogenizing time is 5-10 min.

The emulsion has effects of improving skin micro-ecology and skin physiological state, and can be added into skin care product with skin micro-ecology protection and antiaging effect.

The brown algae yeast fermentation lysate is a product of brown algae fermented by yeast, so that more brown algae oligosaccharides can be obtained, and the fermented yeast lysate contains yeast glucan, nucleotide and other substances, and the effective components can improve the microecology of the aged skin and have better skin repair effect.

The lactobacillus/pear juice fermentation filtrate is obtained by fermenting lactobacillus in pear juice, fructose in the pear juice is utilized to generate certain organic acids such as lactic acid and free amino acid, and the growth of propionibacterium can be better influenced by combining with the added short-chain fatty acid salt. Meanwhile, the lactobacillus can also decompose the components in the pear juice to generate the components beneficial to the skin.

The emulsion takes the brown algae yeast fermentation lysate, the lactobacillus/pear juice fermentation filtrate and the short-chain fatty acid salt as main effective components, brown algae oligosaccharide and yeast glucan contained in the brown algae yeast fermentation lysate and lactic acid and the short-chain fatty acid salt in the lactobacillus/pear juice fermentation filtrate are taken as prebiotics and prebiotics of skin flora and are synergistically acted on skin microecology to influence the growth of the skin flora and play a role in obviously regulating the trend of converting the aged skin flora structure into the young skin flora structure; the metabolites such as nucleotide in the brown algae yeast fermentation lysate and a plurality of amino acids generated by lactobacillus/pear juice fermentation filtrate can be quickly permeated and absorbed due to small molecular weight, and jointly act on in-vitro skin, so that the thickness of the epidermal layer is remarkably increased, the expression of elastin in the dermal layer is promoted, and the physiological state of aged skin is improved. In combination, the present invention primarily utilizes these 3 ingredients to improve the micro-ecology of aging skin and the physiological state of skin. The sucrose ester emulsifier has sucrose end, can promote the growth of skin probiotic-staphylococcus epidermidis as the influencing component of flora, and has positive regulation effect on skin flora. The sucrose ester emulsifier, natural oil, caprylic/capric triglyceride and polyalcohol can also play roles in moistening, moisturizing, emulsifying, stabilizing, homogenizing and the like, and an optimal emulsion formula is prepared, so that the effective components can play an optimal function.

The invention has the beneficial effects that:

1. the skin micro-ecology of the aging population can be obviously improved through the test verification of the population, and the skin physiological state of the aging population is improved.

2. The composition can significantly increase the thickness of the epidermal layer and promote the expression of elastin in the dermal layer.

3. The main functional components in the composition are natural sources, are easy to obtain, are safe and mild, and are convenient to use.

Drawings

FIG. 1 is a comparison graph of facial physiological indexes of different aging degrees of 262 healthy women, note that: in this study, x: p is more than 0.01 and less than or equal to 0.05; **: p is more than 0.001 and less than or equal to 0.01; ***: p is less than or equal to 0.001;

FIG. 2 is a histogram (genus level) of the bacterial species composition, Chao index (A) and Shannon index (B);

FIG. 3 is a diagram of the analysis of differences in species composition (genus level) and species composition for different groups of bacterial species;

FIG. 4 is a graph of the effect of different samples on EX vivo skin tissue morphology;

FIG. 5 is a graph showing the effect of different samples on the expression of elastin in Ex vivo skin tissue of EX vivo;

FIG. 6 shows the composition of bacterial species (genus level) on the skin of the test subjects 7 days after use;

FIG. 7 is a comparison of the relative abundance of skin bacterial species before and after use of the test product (genus level);

FIG. 8 is a graph of the change in skin wrinkles SEw and dermis echogenicity before and after use of the test product: the significance labeling method comprises the following steps: (v.D0); "n.s." means no statistical difference, p > 0.05; "+" indicates significant difference, p is more than or equal to 0.0001 and less than 0.05;

FIG. 9 is a graph of variation in fine line length and fine line count before and after use of a test product; the significance labeling method comprises the following steps: (v.D0); "n.s." means no statistical difference, p > 0.05; "+" indicates significant difference, p is more than or equal to 0.0001 and less than 0.05;

FIG. 10 is a graph showing the change in the color a of the subcutaneous red zone before and after the test product was used; the significance labeling method comprises the following steps: (v.D0); "n.s." means no statistical difference, p > 0.05; "+" indicates significant difference, p is more than or equal to 0.0001 and less than 0.05;

fig. 11 is a diagram of wrinkle-removing effect of effective example 1 (VC98 USB photograph);

fig. 12 is a diagram of wrinkle-removing effect of effective example 2 (VC98 USB photograph);

FIG. 13 is a graph showing the effect of relaxing Red-removal in working example 1 (Visia-CR Cross Polarized Light Red Areas mode photograph);

FIG. 14 is a graph showing the effect of relaxing Red-removal in working example 2 (Visia-CR Cross Polarized Light Red Areas model photograph).

Detailed Description

In order to better understand the invention, the following examples further illustrate the content of the invention, but the content of the invention is not limited to the following examples, and the examples should not be construed as limiting the scope of the invention.

The preparation method of the brown algae yeast fermentation lysate used in this example:

1) soaking, cleaning and crushing brown algae into 100 meshes, and then performing plasma beam sterilization treatment with the irradiation intensity of 6000kGy for 40 min;

2) mixing 1) with purified water 3 times of its mass, inoculating 2 × 10 Saccharomyces cerevisiae suspension⁵Fermenting cfu/ml for 72h at 36 ℃ to obtain fermentation liquor;

3) breaking the cell wall of the thallus in the fermentation liquor by a high-pressure homogenizer, crushing to obtain brown algae yeast fermentation crude extract, filtering with 400 meshes to obtain brown algae yeast fermentation lysate, and adjusting the dry matter content (the dry solid matter content) to be about 10%.

The method for preparing the lactobacillus/pear juice fermentation filtrate used in this example: inoculating lactobacillus 1 × 10 in pear juice culture medium⁶Performing fermentation culture on cfu/ml at the fermentation temperature of 37 ℃ for 48 h; and after the fermentation is finished, performing centrifugal separation at 8000r/min, and taking supernatant fluid, namely lactobacillus/pear juice fermentation filtrate. The pear juice culture medium is as follows: washing pear, juicing with a juicer (no water is added during juicing) to obtain fresh squeezed pear juice, adding 20% of fresh squeezed pear juice into MRS culture medium, and sterilizing to obtain pear juice culture medium.

Example 1: preparation of the emulsion

Heating and mixing 1.0g of sucrose laurate, 4.0g of butanediol and 1.5g of hexanediol, adding 1.94g of purified water, and cooling to 38 ℃ to form a liquid I; mixing 2.0g of meadowfoam seed oil, 2.0g of malus oil and 2.5g of caprylic/capric triglyceride to form a liquid II; adding 2.0g of brown algae yeast fermentation lysate (0.2 g based on dry matter), 0.06g of calcium propionate and 3.0g of lactobacillus/pear juice fermentation liquor into the mixed liquor of the first liquid, and stirring and mixing uniformly to form a third liquid; slowly adding the second liquid into the third liquid, and homogenizing at 10000r/min for 8min to form a uniform emulsion.

Example 2: preparation of the emulsion

Heating and mixing 1.2g of sucrose laurate, 3.0g of pentanediol and 2.5g of glycerol, adding 1.45g of purified water, and cooling to 38 ℃ to form a liquid I; mixing 2.0g camellia seed oil, 2.0g malus oil and 3.0g caprylic/capric triglyceride to form a second liquid; adding 2.0g of brown algae yeast fermentation lysate (0.2 g based on dry matter), 0.05g of calcium propionate and 2.8g of lactobacillus/pear juice fermentation liquor into the mixed liquor of the first liquid, and stirring and mixing uniformly to form a third liquid; slowly adding the second liquid into the third liquid, and homogenizing at 10000r/min for 8min to form a uniform emulsion.

Example 3: preparation of the emulsion

Heating and mixing 1.5g of sucrose laurate, 0.3g of caprylyl glycol and 4.0g of glycerol, adding 2.93g of purified water, and cooling to 38 ℃ to form a liquid I; mixing 2.0g camellia oil, 2.0g horse milkweed oil and 3.0g caprylic/capric triglyceride to form a second liquid; adding 2.2g of brown algae yeast fermentation lysate (0.22 g based on dry matter), 0.07g of calcium propionate and 2.0g of lactobacillus/pear juice fermentation liquor into the mixed liquor of the first liquid, and stirring and mixing uniformly to form a third liquid; slowly adding the second liquid into the third liquid, and homogenizing at 10000r/min for 8min to form a uniform emulsion.

Control group 1: brown algae yeast fermentation lysate group

The same procedure as in example 1 was repeated except that 5.0g of the brown algae yeast fermentation lysate (0.5 g in terms of dry matter) was used and the lactobacillus/pear juice fermentation broth was not used, to obtain a brown algae yeast fermentation lysate group (emulsion).

Control group 2: lactobacillus/pear juice fermentation filtrate group

The lactobacillus/pear juice fermentation filtrate group (emulsion) was obtained by using 5.0g of lactobacillus/pear juice fermentation broth without using the phaeophyceae yeast fermentation lysate and the other components, the amounts and the preparation method as in example 1.

The emulsions of example 1 and comparative examples 1 to 2 were used in the following test examples, in which the above three emulsions were diluted to 5. mu.g/mL with DMEM/F12(1:1) culture solution, respectively, for the following test examples 2 and 3.

Test example 1: detection and analysis of skin physiological parameters and skin microbial flora of different aging populations

262 healthy women with the ages of 18-65 are selected, a Visia-CR facial image analyzer is used for wrinkle detection, and the healthy women are divided into three groups according to wrinkle conditions (respectively marked as W0, W1 and W2 grades, W0 grade: no wrinkle, W1 grade: slight wrinkle, W2 grade: much wrinkle and deep wrinkle, and the volunteers in the three groups after classification are 134 persons in W0 grade, 105 persons in W1 grade and 23 persons in W2 grade). The three groups of adults were subjected to the following skin physiological parameter detection and skin microbial flora detection.

Skin physiological parameter detection: use of

CM 825 skin moisture tester,

TM 300 skin water loss tester,

The SM 815 skin oil tester and the Cutomer dual MPA580 skin elasticity tester respectively measure the skin moisture content, the skin percutaneous water loss rate (TEWL), the sebum amount, the elasticity and the tightness of corresponding areas below the cheekbones on the left side and the right side of a volunteer. The results of the detection are shown in FIG. 1.

Detection of skin microbial flora: 3 sterile cotton swab swabs are dipped in sterile normal saline (0.9 percent of normal sodium chloride solution-0.1 percent of Tween-20), the two symmetrical positions on the left side and the right side of the face of a volunteer and the three positions in the middle of the forehead are repeatedly wiped for at least 15 times, the cotton swab swabs are rotated during wiping, and the sampling area is 3cm multiplied by 3 cm. After the collection is finished, 16S rDNA sequencing is carried out, and the differences of the diversity (Chao index (A) and Shannon index) of the skin microbial flora and the composition (belonging level) of the flora of different wrinkle groups are seen through analysis. The analysis results are shown in fig. 2 and 3.

The test finds that the facial skin flora of the tested population has the highest proportion of propionibacterium and has positive correlation with the sebum quantity. As wrinkles increased, there was a greater difference in facial skin microflora and flora ratios, with the slightly wrinkled and wrinkled groups and the abundance of the deep group propionibacterium (Cutibacterium) significantly decreased compared to the wrinkle-free group (see fig. 3).

Test example 2: effect of the emulsion on the morphology of skin tissue

EX vivo skin tissue cultured in vitro was used as the subject and divided into 4 groups of 3 replicates each, 4 groups were subjected to UVR irradiation (wherein UVA was 30J/cm)²，UVB 50mJ/cm²Irradiation for 4 days), irradiated with UVR without any treatment as NC group, and irradiated with UVR, followed by brown algae yeast fermentation lysate (5. mu.g/mL) and lactobacillus/pear juice fermentation filtrate (5. mu.g/mL), respectivelymL), emulsion of the present invention (5. mu.g/mL) acted as 3 experimental groups, and 2. mu.L/cm each was administered to the surface of EX vivo skin tissue²Then by observing the tissue H&E staining to evaluate the effect of the emulsion.

The test results are shown in fig. 4, compared with the NC control group, the phaeophyceae yeast fermentation lysate, the lactobacillus/pear juice fermentation filtrate and the emulsion group can obviously thicken the epidermal living cells, obviously increase the dermal fibroblast density, and reduce vacuoles; and the emulsion component has better promotion effect than the single components of the brown algae yeast fermentation lysate and the lactobacillus/pear juice fermentation filtrate.

Test example 3: effect of emulsions on elastin Synthesis

EX vivo skin tissue cultured in vitro was used as the subject and divided into 4 groups of 3 replicates each, 4 groups were subjected to UVR irradiation (wherein UVA30J/cm²，UVB50mJ/cm²Irradiation for 4 days), the test pieces were treated with UVR irradiation without any treatment as NC groups, and then with UVR irradiation, the brown algae yeast fermentation lysate (5. mu.g/mL), lactobacillus/pear juice fermentation filtrate (5. mu.g/mL), and the emulsion of the present invention (5. mu.g/mL) were used as 3 test groups, and 2. mu.L/cm of each of the solutions was administered to the surface of EX vivo isolated skin tissue²The effect of the emulsion was evaluated by measuring the elastin content in the tissue.

As shown in fig. 5, compared to the NC control group, both the phaeophyceae yeast fermentation lysate and the emulsion group can promote the increase of elastin content in EX vivo skin tissue (brown color area increases after immunohistochemical staining), and the emulsion group has better promotion effect than the phaeophyceae yeast fermentation lysate and lactobacillus/pear juice fermentation filtrate single component.

Test example 4: skin micro-ecology improvement of aged skin by emulsion

Preparing 0.4 wt% of an acrylic copolymer aqueous solution, adding 20g of the emulsion prepared in example 1 into 85.0g of the acrylic copolymer aqueous solution, and stirring and mixing uniformly to form an essence product.

The beneficial effects of the essence containing the emulsion on skin micro-ecology are evaluated by taking a Chinese healthy female population of 30-40 years old as a subject to count 33 persons in total, continuously using the essence containing the emulsion for 7 days in the morning and evening after a tester cleans the face every day, and measuring indexes such as change of skin flora before and after a volunteer product is used.

The results show that: the increase in the abundance of propionibacterium in the test population (fig. 6-7, table 1) using the serum containing the emulsion gradually transformed the condition of the skin microflora with signs of aging into a rejuvenated skin microflora.

TABLE 1 comparison of the relative abundance of skin bacterial species before and after use of the test products (genus level)

Test example 5: skin physiological state change of aged skin caused by lotion

A total of 33 people are selected from Chinese healthy female people of 30-40 years old, and the testers are allowed to continuously use the essence containing the lotion for 28 days and 56 days respectively after cleaning the face every day. The beneficial effect of the essence containing the emulsion on the physiological state of the skin is evaluated by measuring indexes such as the change of the physiological state of the skin before and after the application of the volunteer product. Photographs of the face of the subject were taken and parameters such as skin wrinkles, dermal echo intensity, fine line length, fine line count, subcutaneous red zone color a value, etc. were measured. And comparing the data results and the evaluation results before and after the product is used by a statistical test method to judge whether the statistical difference exists.

The test results are shown in fig. 8-10, and show that skin wrinkles SEw are significantly reduced after 28 days of use, indicating that the tested product has wrinkle-removing efficacy after 28 days of use; after 56 days of use, the skin wrinkles SEw, the fine line score, the fine line length, the fine line count and the subcutaneous red zone color a are all obviously reduced, and the echo intensity of the dermis is obviously increased, which shows that the tested product has the efficacies of removing wrinkles, resisting aging, relieving and the like after 56 days of use.

2 testers with obvious wrinkle-removing and anti-aging effects after using the product for 28 days are selected, and effective examples are shown in figures 11-12. 2 test persons with obvious effects of relieving and removing red after using the product for 28 days are selected, and effective examples are shown in figures 13 and 14. As can be seen from the figure: the effect is obvious.

Claims

1. An emulsion for improving the microecology and physiological state of aged skin is characterized by at least comprising three effective components of phaeophyceae yeast fermentation lysate, lactobacillus/pear juice fermentation filtrate and short-chain fatty acid salt.

2. The emulsion according to claim 1, wherein the three active ingredients are present in the emulsion in amounts selected from the group consisting of: every 20g of the emulsion contains 0.1-0.3g of the phaeophyceae fermentation lysate, 0.5-8.0g of lactobacillus/pear juice fermentation filtrate and 0.05-0.1g of short-chain fatty acid salt, and the dosage of the phaeophyceae fermentation lysate is calculated by dry matter.

3. The emulsion for improving the skin micro-ecology and skin physiology under aging of claim 2, wherein the three effective ingredients are used in the emulsion in amounts of: each 20g of the emulsion contains 0.2g of the phaeophyceae yeast fermentation lysate, 3.0g of lactobacillus/pear juice fermentation filtrate and 0.06g of short-chain fatty acid salt.

4. An emulsion for improving the skin micro-ecology and skin physiology of aging according to any one of claims 1-3, wherein the phaeophyceae fermentation lysate is: inoculating yeast into brown algae aqueous solution for fermentation, breaking the cell wall of fermentation liquor by a high-pressure homogenizer, crushing the thallus, and filtering by a 200-mesh and 400-mesh filter to obtain the brown algae water-soluble powder.

5. An emulsion for improving the skin micro-ecology and skin physiology of aging according to any one of claims 1-3 wherein the lactobacillus/pear juice fermentation filtrate is: after lactobacillus is fermented and cultured in a pear juice culture medium, centrifugal separation is carried out, and supernatant is taken to obtain the lactobacillus beverage, wherein the pear juice culture medium is prepared by adding 20 +/-5% of fresh squeezed pear juice into an MRS culture medium.

6. An emulsion for improving the skin micro-ecology and the skin physiology condition of aging according to any one of claims 1-3, wherein the short chain fatty acid salt is one or two of lactate, butyrate, propionate and isobutyrate.

7. The emulsion according to any one of claims 1 to 3, which is an emulsion prepared by adding sucrose ester emulsifier, natural oil, caprylic/capric triglyceride, polyhydric alcohol and water, wherein each 20g of the emulsion contains 0.1 to 0.3g of phaeophyceae yeast fermentation lysate, 0.5 to 8.0g of lactobacillus/pear juice fermentation filtrate, 0.05 to 0.1g of short-chain fatty acid salt, 0.2 to 2.0g of sucrose ester emulsifier, 0.5 to 10.0g of natural oil, 1.0 to 4.0g of caprylic/capric triglyceride, 0.5 to 10.0g of polyhydric alcohol and the balance of water.

8. The emulsion for improving skin micro-ecology and skin physiology according to claim 7, wherein the sucrose lipid emulsifier is one or two of sucrose laurate, sucrose palmitate and sucrose stearate;

the natural oil is one or two of white Potentilla seed oil, Malula oil, oleum Camelliae Japonicae, oleum Cocois, and bitter almond oil;

9. The process for the preparation of an emulsion according to claim 7, comprising the steps of:

s2: mixing natural oil and caprylic/capric triglyceride to form a liquid II;

s4: slowly adding the liquid II into the liquid III, and homogenizing at a high speed to form a uniform emulsion.

10. Use of the emulsion according to any one of claims 1 to 3 for the addition to an anti-aging skin care product which is friendly to the skin micro-ecology for the improvement of the micro-ecology of aging skin and the physiological state of the skin.