CN112587471A - Composition for improving skin micro-ecology and preparation method and application thereof - Google Patents
Composition for improving skin micro-ecology and preparation method and application thereof Download PDFInfo
- Publication number
- CN112587471A CN112587471A CN202011492057.XA CN202011492057A CN112587471A CN 112587471 A CN112587471 A CN 112587471A CN 202011492057 A CN202011492057 A CN 202011492057A CN 112587471 A CN112587471 A CN 112587471A
- Authority
- CN
- China
- Prior art keywords
- skin
- composition
- ecology
- improving
- lactobacillus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/981—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of mammals or bird
- A61K8/986—Milk; Derivatives thereof, e.g. butter
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
Abstract
The invention relates to a composition for improving skin microecology, a preparation method and application thereof, wherein the composition for improving skin microecology comprises the following components: inulin/yogurt powder mixture, flos Rosae Davuricae extract, acetyl heptapeptide-4, Bacillus fermentation product, lactobacillus fermentation lysate, and lactobacillus/eriodictyon fermentation product extract. The invention creatively combines the six components which can be degraded and transdermally absorbed by the skin and supply nutrition to the skin, realizes the synergistic interaction effect and plays the obvious effect of improving the skin barrier function and the skin surface microbial community distribution. The composition is added into cosmetic formulations such as astringent, lotion, cream and the like, and can remarkably improve the skin micro-ecological improving effect of the cosmetics.
Description
Technical Field
The invention belongs to the technical field of cosmetics, and particularly relates to a composition for improving skin microecology and a preparation method and application thereof, in particular to a composition for improving skin barrier function and skin surface microbial population distribution and a preparation method and application thereof.
Background
It is well known that the skin, the largest organ of the human body, protects our body against external environmental insults. The stratum corneum in the epidermis is composed of a dense layer of cells, and plays an extremely important barrier role for the human body due to its compact structure.
With the development and improvement of the technology, the skin is layered more and more carefully, and in addition to needing to pay attention to the physical barrier of the skin which is often contacted, the newly introduced skin microbial barrier is not separated from the health of the skin in daily skin care. As a human body surface barrier-skin, there are often some microorganisms present, some of which may be parasitic on the skin of the human body for a longer period of time as a result of long-term adaptation, known as the normal skin microflora, also known as the normal skin flora. The normal flora and the host macroorganisms and the external environment form a habitat, microorganisms in the habitat colonize certain organs of the macroorganisms to form microbial localization, and the skin is also one of the localizations. This interdependence constitutes an ecosystem. The skin has 'good flora' which is the crucial point of the good state of our skin, and the microbial barrier and the skin physical barrier play the role of protecting our skin at the same time.
However, with the development of modern society, the daily working and rest time of people is not regular, the environmental conditions are worse and worse, and the adverse effects on the skin are worse and worse due to the mental pressure from all aspects and the frequent cosmetic and makeup removing habits. The physical barrier and the microbial barrier of the skin are damaged, so that the skin is subjected to various troubles, the skin becomes fragile, the current skin barrier repair method mainly focuses on the repair of the physical barrier, and moisture is retained on the skin by externally coating composite components of humectants such as grease blocking moisturizers, hydrophilic matrix moisture absorbing moisturizers, hydrating moisturizers and the like, so that the method cannot fundamentally solve the problem of repairing the skin barrier dysfunction, and has little effect particularly on the problem of the damage of the microbial barrier.
CN111265468A discloses a skin double-barrier repair composition and application thereof, wherein prebiotics, probiotics and microbial metabolites in skin microbial components are simulated by mutual matching of yeast bifida fermentation product filtrate, oat fermentation filtrate and skin-like lipid complex, so that the skin microbial barrier is effectively stabilized and balanced; the skin lipid complex and the oat kernel oil mainly repair a skin brick wall structure and improve the composition of stratum corneum lipid so as to achieve the aim of repairing a physical barrier of the skin, and the skin microbial barrier and the physical barrier of the skin are repaired simultaneously through the mutual matching of the two barrier repairing components, so that the skin is helped to reestablish the stratum corneum with a complete structure and establish a healthy and good microbial colony ecosphere.
CN110934804A discloses a skin micro-ecological nursing facial mask added with distiller's grains, which comprises the following substances: the alcohol dreg facial mask provided by the invention is based on skin microbiology, improves the skin microorganism problem, and can effectively balance the skin microorganism population and the quantity, thereby leading the skin to return to a healthy state.
However, there are few strategies in the prior art for better improving both the microbial barrier and the physical barrier of the skin, and therefore, it is of great interest to develop a product for improving the skin barrier function and the skin surface microbial flora.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide a composition for improving skin micro-ecology and a preparation method and application thereof, in particular to a composition for improving skin barrier function and skin surface microbial community and a preparation method and application thereof.
In order to achieve the purpose, the invention adopts the following technical scheme:
in a first aspect, the present invention provides a composition for improving skin micro-ecology, comprising: inulin/yogurt powder mixture, flos Rosae Davuricae extract, acetyl heptapeptide-4, Bacillus fermentation product, lactobacillus fermentation lysate, and lactobacillus/eriodictyon fermentation product extract.
The invention creatively combines the six components of the inulin/yoghurt powder mixture, the rosa multiflora flower extract, the acetyl heptapeptide-4, the bacillus fermentation product, the lactobacillus fermentation lysate and the lactobacillus/eriodictyon fermentation product extract which can be degraded and transdermally absorbed by skin and supply nutrition to the skin, thereby realizing the synergistic effect and playing the obvious effects of improving the skin barrier function and the microbial population distribution on the surface of the skin. The composition is added into cosmetic formulations such as astringent, lotion, cream and the like, and can remarkably improve the skin micro-ecological improving effect of the cosmetics.
Preferably, the composition for improving skin micro-ecology further comprises any one or a combination of at least two of a preservative, a penetration enhancer, a thickening agent, a pH regulator or water; the combination of at least two of the above components, such as the combination of a preservative and a penetration enhancer, the combination of a thickener and a pH regulator, the combination of a penetration enhancer, a thickener and a pH regulator, and the like, can be selected in any combination manner, and are not described in detail herein.
Preferably, the composition for improving skin micro-ecology further comprises a preservative, a penetration enhancer, a thickener, a pH regulator and water.
Preferably, the composition for improving skin micro-ecology further comprises 1, 2-pentanediol, carbomer, aminomethyl propanol and water.
The 1, 2-pentanediol has the functions of a preservative and a penetration enhancer, the carbomer plays a role of a thickening agent, and the aminomethyl propanol plays a role of a pH regulator.
Preferably, the composition for improving skin microecology comprises the following components in parts by weight: inulin/yogurt powder mixture 0.1-5 parts, flos Rosae Davuricae extract 0.0006-0.03 part, acetyl heptapeptide-40.00005-0.0025 part, Bacillus fermentation product 0.00011-0.0055 part, lactobacillus fermentation lysate 0.0075-0.375 part, and lactobacillus/Halloween fermentation product extract 0.098-4.9 parts.
When the composition for improving skin micro-ecology according to the present invention is combined in the above-mentioned specific mass ratio, it has more significant effects in improving skin barrier function and skin surface microflora distribution.
The inulin/yoghurt mix may be present in an amount of 0.1 parts, 0.2 parts, 0.3 parts, 0.5 parts, 0.8 parts, 1 part, 2 parts, 4 parts, 5 parts, etc.
The rosa centifolia flower extract can be 0.0006 part, 0.001 part, 0.005 part, 0.01 part, 0.012 part, 0.015 part, 0.02 part, 0.025 part, 0.03 part and the like in parts by weight.
The acetyl heptapeptide-4 may be 0.00005 parts, 0.0001 parts, 0.0005 parts, 0.001 parts, 0.0012 parts, 0.0015 parts, 0.002 parts, 0.0022 parts, 0.0025 parts, etc. by weight.
The weight portion of the bacillus fermentation product can be 0.00011 portion, 0.0002 portion, 0.0005 portion, 0.0011 portion, 0.0033 portion, 0.0040 portion, 0.0045 portion, 0.0050 portion, 0.0055 portion and the like.
The lactobacillus fermentation lysate can be in the weight portion of 0.0075 parts, 0.01 parts, 0.03 parts, 0.05 parts, 0.075 parts, 0.1 parts, 0.225 parts, 0.3 parts, 0.375 parts, etc.
The lactobacillus/eriodictyon fermentation product extract may be present in an amount of 0.098 parts, 0.1 parts, 0.5 parts, 1 part, 1.5 parts, 2 parts, 2.94 parts, 4 parts, 4.9 parts, etc. by weight.
The specific values in the above ranges can be selected, and are not described in detail herein.
Preferably, the composition for improving skin microecology comprises the following components in parts by weight: inulin/yogurt powder mixture 0.3-0.5 parts, flos Rosae Davuricae extract 0.0006-0.012 parts, acetyl heptapeptide-40.0005-0.0015 parts, Bacillus fermentation product 0.0011-0.0033 parts, lactobacillus fermentation lysate 0.075-0.225 parts, and lactobacillus/Halloween fermentation product extract 0.98-2.94 parts.
In a second aspect, the present invention provides a method for preparing the composition for improving skin micro-ecology as described above, the method comprising: mixing inulin/yogurt powder mixture, flos Rosae Davuricae extract, acetyl heptapeptide-4, Bacillus fermentation product, lactobacillus fermentation lysate and lactobacillus/eriodictyon fermentation product extract.
Preferably, the preparation method comprises:
(1) uniformly mixing the inulin/yoghurt powder mixture with 1, 2-pentanediol to obtain a mixture A; mixing carbomer and water, heating, keeping the temperature, and cooling to obtain solution B;
(2) mixing flos Rosae Davuricae extract, acetyl heptapeptide-4, Bacillus fermentation product, lactobacillus fermentation lysate and lactobacillus/herba Saint holtziae fermentation product extract with aminomethyl propanol, mixture A, and solution B to obtain the composition for improving skin microecology.
The preparation method of the composition for improving skin microecology is simple and easy to operate, and is very suitable for industrial production.
Further preferably, the inulin/acid milk powder mixture and the 1, 2-pentanediol are uniformly mixed in the step (1) under stirring, the stirring speed is 100-200rpm, such as 100rpm, 120rpm, 150rpm, 180rpm or 200rpm, and the like, and the time is 1-5min, such as 1min, 2min, 3min, 4min or 5min, and other specific points in the above range can be selected, and thus, the description is omitted.
The inulin/yoghurt powder mixture which is a solid component and is difficult to dissolve in water is firstly mixed and dissolved with the 1, 2-pentanediol, so that the inulin/yoghurt powder mixture is convenient to be uniformly mixed with other components subsequently, and the efficacy of the inulin/yoghurt powder mixture is more remarkably exerted.
Preferably, the heating in step (1) is heating to 80-90 ℃, for example, 80 ℃, 82 ℃, 84 ℃, 85 ℃, 86 ℃, 88 ℃ or 90 ℃, etc., preferably 84-86 ℃, and other specific values within the above range can be selected, and are not repeated herein.
Preferably, the heat preservation time in the step (1) is 20-40min, for example, 20min, 25min, 30min, 35min or 40min, and other specific values in the above range can be selected, and are not repeated herein.
Preferably, the temperature reduction in step (1) refers to temperature reduction to 40-50 ℃, for example, 40 ℃, 42 ℃, 44 ℃, 45 ℃, 46 ℃, 48 ℃ or 50 ℃, etc., preferably 44-46 ℃, and other specific values within the above range can be selected, and are not described herein again.
In the composition, the components of the rosa multiflora flower extract, the acetyl heptapeptide-4, the bacillus fermentation product, the lactobacillus fermentation lysate and the lactobacillus/eriodictyon fermentation product extract are added into a mixing system at the specific temperature, so that the activity of the components can be ensured to the maximum extent, and the stability of the mixing system can be ensured.
Preferably, the mixing in the step (2) is performed under stirring, the stirring speed is 800-1000rpm, such as 800rpm, 850rpm, 900rpm, 950rpm or 1000rpm, and the like, and the time is 5-10min, such as 5min, 6min, 7min, 8min, 9min or 10min, and other specific values in the above range can be selected, and thus, the description is omitted.
In a third aspect, the present invention provides the use of a composition as described above for improving skin micro-ecology in the manufacture of a skin care product.
In a fourth aspect, the present invention provides a skin care product, where the skin care product includes the composition for improving skin microecology as described above, and the content of the composition for improving skin microecology in the skin care product is 10-40% by mass, for example, 10%, 15%, 20%, 25%, 30%, 35%, or 40%, etc., and other specific values within the above range can be selected, and are not described herein again.
Preferably, the skin care product comprises the following components in percentage by mass:
the component A comprises: 6.0-8.0% of glycerol, 1-5% of 1, 2-pentanediol, 0.18-0.2% of carbomer and the balance of water;
and B component: 10-40% of composition for improving skin microecology;
and C, component C: 0.1-12% of aminomethyl propanol.
Compared with the prior art, the invention has the following beneficial effects:
the invention creatively combines the six components of the inulin/yoghurt powder mixture, the rosa multiflora flower extract, the acetyl heptapeptide-4, the bacillus fermentation product, the lactobacillus fermentation lysate and the lactobacillus/eriodictyon fermentation product extract which can be degraded and transdermally absorbed by skin and supply nutrition to the skin, thereby realizing the synergistic effect and playing the obvious effects of improving the skin barrier function and the microbial population distribution on the surface of the skin. The composition is added into cosmetic formulations such as astringent, lotion, cream and the like, and can remarkably improve the skin micro-ecological improving effect of the cosmetics.
Detailed Description
To further illustrate the technical means and effects of the present invention, the following further describes the technical solution of the present invention with reference to the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.
The inulin/yoghurt mix referred to in the examples, comparative examples, application examples and comparative application examples of the following formula was used the Yourcene blannce CQ (PFR) U/A product (100% strength) from Qiwangton;
flos Rosae Davuricae extract uses ROSALISS product (concentration of 0.6%) from Ashland;
acetyl heptapeptide-4 was used as FENSEBIOME peptide solvent product (concentration 0.05%) from Lipotec;
the Bacillus fermentation product is obtained from UNICLAY BIOTECH Ingredient (0.11%) in Luobo;
the lactobacillus fermentation lysate used was the BIOTILYS product from the chlorocaceae (concentration 7.5%);
Lactobacillus/Halloween fermentation product extract was obtained using the product VanCare YB Santa (concentration 98%) from VanAArde;
as the aminomethylpropanol solution, PC 2000 (95% strength) was used, which was obtained from ANGUS in the United states.
Example 1
The embodiment provides a composition for improving skin micro-ecology, which comprises the following components: 4% inulin/yogurt powder mixture, 1% rose flower extract solution, 2% acetyl heptapeptide-4 solution, 2% bacillus fermentation product solution, 2% lactobacillus fermentation lysate solution, 2% lactobacillus/eriodictyon fermentation product extract solution, 4% 1, 2-pentanediol, 0.12% carbomer, 0.08% aminomethyl propanol solution, and the balance water.
The preparation method comprises the following steps:
(1) mixing and dissolving the inulin/acid milk powder mixture and 1, 2-pentanediol at the stirring speed of 150rpm at 25 ℃, and stirring for 3min to obtain a mixture A;
(2) mixing carbomer and water, heating to 85 deg.C, keeping the temperature for 30min, and cooling to 45 deg.C to obtain solution B;
(3) mixing flos Rosae Davuricae extract, acetyl heptapeptide-4, Bacillus fermentation product, lactobacillus fermentation lysate, lactobacillus/eriodictyon fermentation product extract, aminomethyl propanol, mixture A, and solution B at stirring speed of 900rpm, and stirring for 8min to obtain the composition for improving skin microecology.
Example 2
The embodiment provides a composition for improving skin micro-ecology, which comprises the following components: 1% inulin/yogurt powder mixture, 4% rose flower extract solution, 1% acetyl heptapeptide-4 solution, 4% bacillus fermentation product solution, 1% lactobacillus fermentation lysate solution, 4% lactobacillus/eriodictyon fermentation product extract solution, 4% 1, 2-pentanediol, 0.2% carbomer, 0.12% aminomethyl propanol solution, and the balance water.
The preparation method comprises the following steps:
(1) mixing and dissolving the inulin/acid milk powder mixture and 1, 2-pentanediol at a stirring speed of 100rpm at 25 ℃, and stirring for 5min to obtain a mixture A;
(2) mixing carbomer and water, heating to 86 deg.C, keeping the temperature for 20min, and cooling to 46 deg.C to obtain solution B;
(3) mixing flos Rosae Davuricae extract, acetyl heptapeptide-4, Bacillus fermentation product, lactobacillus fermentation lysate, lactobacillus/eriodictyon fermentation product extract, aminomethyl propanol, mixture A, and solution B at stirring speed of 1000rpm, and stirring for 5min to obtain the composition for improving skin microecology.
Example 3
The embodiment provides a composition for improving skin micro-ecology, which comprises the following components: 5% inulin/yogurt powder mixture, 1% flos Rosae Davuricae extract solution, 5% acetyl heptapeptide-4 solution, 1% Bacillus fermentation product solution, 5% lactobacillus fermentation lysate solution, 1% lactobacillus/eriodictyon fermentation product extract solution, 4% 1, 2-pentanediol, 0.1% carbomer, 0.10% aminomethyl propanol solution, and water in balance.
The preparation method comprises the following steps:
(1) mixing and dissolving the inulin/acid milk powder mixture and 1, 2-pentanediol at the stirring speed of 200rpm at 25 ℃, and stirring for 3min to obtain a mixture A;
(2) mixing carbomer and water, heating to 84 deg.C, keeping the temperature for 40min, and cooling to 44 deg.C to obtain solution B;
(3) mixing flos Rosae Davuricae extract, acetyl heptapeptide-4, Bacillus fermentation product, lactobacillus fermentation lysate, lactobacillus/eriodictyon fermentation product extract, aminomethyl propanol, mixture A, and solution B at stirring speed of 800rpm, and stirring for 10min to obtain the composition for improving skin microecology.
Example 4
This example provides a skin micro-ecological improving composition having the same composition as in example 1. The preparation method is different from the example 1 only in that the temperature reduction treatment is not carried out in the step (2), and other conditions are kept consistent.
Example 5
This example provides a skin micro-ecological improving composition having the same composition as in example 1. The preparation method is different from the example 1 only in that the temperature is reduced to 50 ℃ in the step (2), and other conditions are kept consistent.
Example 6
This example provides a skin micro-ecological improving composition having the same composition as in example 1. The preparation method is different from the example 1 only in that the temperature is reduced to 40 ℃ in the step (2), and other conditions are kept consistent.
Comparative example 1
This comparative example provides a composition for improving skin micro-ecology, which is different from example 1 only in that it does not contain inulin/yogurt powder mixture, and the mass percentage of the rosa centifolia extract solution is 5%, and the other components and contents are kept unchanged. The preparation process was identical to example 1.
Comparative example 2
This comparative example provides a composition for improving skin micro-ecology, which is different from example 1 only in that it does not contain a rosa centifolia flower extract solution, and the inulin/yogurt powder mixture has a mass percentage of 5%, and the other components and contents are kept unchanged. The preparation process was identical to example 1.
Comparative example 3
The comparative example provides a composition for improving skin micro-ecology, which is different from example 1 only in that it does not contain acetyl heptapeptide-4 solution, and the mass percentage content of the bacillus fermentation product solution is 4%, and other components and contents are kept unchanged. The preparation process was identical to example 1.
Comparative example 4
This comparative example provides a composition for improving skin micro-ecology, which is different from example 1 only in that it does not contain a bacillus fermentation product solution, and the mass percentage of the acetyl heptapeptide-4 solution is 4%, and the other components and contents are kept unchanged. The preparation process was identical to example 1.
Comparative example 5
This comparative example provides a composition for improving skin micro-ecology, which is different from example 1 only in that it does not contain a lactobacillus fermentation lysate solution, and the mass percentage of a lactobacillus/eriodictyon fermentation product extract solution is 4%, and the other components and contents are kept unchanged. The preparation process was identical to example 1.
Comparative example 6
This comparative example provides a composition for improving skin micro-ecology, which is different from example 1 only in that it does not contain the lactobacillus/eriodictyon fermentation product extract solution, and the mass percentage of the lactobacillus fermentation lysate solution is 4%, and the other components and contents are kept unchanged. The preparation process was identical to example 1.
Application example 1
The application example provides a skin care product for improving skin microecology, which comprises the following components in percentage by mass:
the component A comprises: 7.0% of glycerol, 4% of 1, 2-pentanediol, 0.15% of carbomer and the balance of water;
and B component: aminomethyl propanol 0.09%.
And C, component C: the composition for improving skin micro-ecology obtained in example 1 was 10%.
The preparation method comprises the following steps:
(1) mixing and stirring the raw materials of the component A, heating to 85 ℃, preserving heat for 20min, and cooling to 45 ℃;
(2) then adding the component B, and stirring for 10 min;
(3) and finally, adding the component C, and stirring for 10min to obtain the composition.
Application example 2
The present application example provides a skin care product for improving skin micro ecology, which has the components different from those of application example 1 only in that the composition for improving skin micro ecology prepared in example 1 is added in an amount of 20%, the water content is correspondingly reduced, and other components and contents are kept unchanged. The preparation process is as in application example 1.
Application example 3
The present application example provides a skin care product for improving skin micro-ecology, which has the components different from those of application example 1 only in that the composition for improving skin micro-ecology prepared in example 1 is added in an amount of 30%, the water content is correspondingly reduced, and other components and contents are kept unchanged. The preparation process is as in application example 1.
Application example 4
The present application example provides a skin care product for improving skin micro-ecology, which has the components different from those of application example 1 only in that the composition for improving skin micro-ecology prepared in example 1 is added in an amount of 40%, the water content is correspondingly reduced, and other components and contents are kept unchanged. The preparation process is as in application example 1.
Application example 5
The present application example provides a skin care product for improving skin micro-ecology, which is different from application example 1 in the component only in that the composition for improving skin micro-ecology prepared in example 1 is replaced with the composition for improving skin micro-ecology prepared in example 2, and the addition amount and other components thereof are kept unchanged. The preparation process is as in application example 1.
Application example 6
The present application example provides a skin care product for improving skin micro-ecology, which is different from application example 1 in the component only in that the composition for improving skin micro-ecology prepared in example 1 is replaced with the composition for improving skin micro-ecology prepared in example 3, and the addition amount and other components thereof are kept unchanged. The preparation process is as in application example 1.
Application example 7
The present application example provides a skin care product for improving skin micro-ecology, which is different from application example 1 in the component only in that the composition for improving skin micro-ecology prepared in example 1 is replaced with the composition for improving skin micro-ecology prepared in example 4, and the addition amount and other components thereof are kept unchanged. The preparation process is as in application example 1.
Application example 8
The present application example provides a skin care product for improving skin micro-ecology, which is different from application example 1 in the component only in that the composition for improving skin micro-ecology prepared in example 1 is replaced with the composition for improving skin micro-ecology prepared in example 5, and the addition amount and other components thereof are kept unchanged. The preparation process is as in application example 1.
Application example 9
The present application example provides a skin care product for improving skin micro-ecology, which is different from application example 1 in the component only in that the composition for improving skin micro-ecology prepared in example 1 is replaced with the composition for improving skin micro-ecology prepared in example 6, and the addition amount and other components thereof are kept unchanged. The preparation process is as in application example 1.
Comparative application examples 1 to 6
The comparative application example provides a skin care product for improving skin micro-ecology, which has the components different from those of application example 1 only in that the composition for improving skin micro-ecology prepared in example 1 is sequentially and respectively replaced with the compositions for improving skin micro-ecology prepared in comparative examples 1 to 6, and the addition amounts and other components thereof are kept unchanged. The preparation process is as in application example 1.
Evaluation test:
(1) testing of skin Water loss (TEWL)
1.1 test Instrument
Skin moisture loss (TEWL) Tewameter TM300 test probe manufactured by Courage + Khazaka, Germany (CK, Germany).
1.2 test methods
160 volunteers are selected, wherein the age is 20-45 years, and the volunteers are half of the male and the female. The groups were randomized into 16 groups of 5 males and 5 females. The using method comprises the following steps: 0.4g of the product (example 1, application examples 1-9 and comparative application examples 1-6) was applied evenly to the face 2 times a day for 14 consecutive days. Prior to the test, subjects were asked to wash their face and to enter a climate controlled room (22. + -. 1 ℃ C., 50% relative humidity) 2 hours after washing and sit still for more than 20min and remain relaxed. The use was continued for 14 days and the subjects were asked to return visits on day 7 (d7), day 14 (d14) of the trial.
1.3 test results are shown in Table 1 (data are presented as mean values):
TABLE 1
From the data results in table 1, it can be seen that: the composition of the invention can reduce the percutaneous water loss of the skin, so that the barrier of the skin is better.
(2) Testing of diversity and abundance of skin microorganism shannon
160 volunteers are selected, wherein the age is 20-45 years, and the volunteers are half of the male and the female. The groups were randomized into 16 groups of 5 males and 5 females. The using method comprises the following steps: 0.4g of the product (example 1, application examples 1-9 and comparative application examples 1-6) was applied evenly to the face 2 times a day for 7 consecutive days.
Analyzing a DNA sequencing result of a 16S rNA gene V3 region and a Shannon diversity index through a microorganism detector and bioinformatics software; the relative abundance of different classes was further evaluated by analyzing the sequencing results of 7 hypervariable regions on the 16S rNA gene. The test results are shown in table 2 (data are presented as mean values):
TABLE 2
Wherein a lower shannon diversity index value indicates a lower microbial diversity, which is often associated with skin unhealthy. Wherein the staphylococcaceae family is associated with skin diseases such as atopic dermatitis and psoriasis; moraxeaceae belongs to the phylum Proteobacteria, gamma Proteobacteria, and has been shown to have potential anti-inflammatory properties, which are associated with immune homeostasis.
From the data results in table 2, it can be seen that: the composition can increase the microbial diversity of the skin, promote the generation of beneficial bacteria and reduce harmful bacteria.
The applicant states that the composition for improving skin micro-ecology and the preparation method and application thereof are described by the above examples, but the invention is not limited to the above examples, that is, the invention is not limited to the above examples. It should be understood by those skilled in the art that any modification of the present invention, equivalent substitutions of the raw materials of the product of the present invention, addition of auxiliary components, selection of specific modes, etc., are within the scope and disclosure of the present invention.
The preferred embodiments of the present invention have been described in detail, however, the present invention is not limited to the specific details of the above embodiments, and various simple modifications may be made to the technical solution of the present invention within the technical idea of the present invention, and these simple modifications are within the protective scope of the present invention.
It should be noted that the various technical features described in the above embodiments can be combined in any suitable manner without contradiction, and the invention is not described in any way for the possible combinations in order to avoid unnecessary repetition.
Claims (10)
1. A composition for improving skin micro-ecology, comprising: inulin/yogurt powder mixture, flos Rosae Davuricae extract, acetyl heptapeptide-4, Bacillus fermentation product, lactobacillus fermentation lysate, and lactobacillus/eriodictyon fermentation product extract.
2. The skin micro-ecology improving composition of claim 1, further comprising any one or a combination of at least two of a preservative, a penetration enhancer, a thickener, a pH adjuster, or water;
preferably, the composition for improving skin micro-ecology also comprises a preservative, a penetration enhancer, a thickening agent, a pH regulator and water;
preferably, the composition for improving skin micro-ecology further comprises 1, 2-pentanediol, carbomer, aminomethyl propanol and water.
3. The skin micro-ecology improving composition according to claim 1 or 2, wherein the skin micro-ecology improving composition comprises, in parts by weight: inulin/yogurt powder mixture 0.1-5 parts, flos Rosae Davuricae extract 0.0006-0.03 part, acetyl heptapeptide-40.00005-0.0025 part, Bacillus fermentation product 0.00011-0.0055 part, lactobacillus fermentation lysate 0.0075-0.375 part, and lactobacillus/Halloween fermentation product extract 0.098-4.9 parts.
4. The skin micro-ecology improving composition according to any one of claims 1 to 3, wherein the skin micro-ecology improving composition comprises, in parts by weight: inulin/yogurt powder mixture 0.3-0.5 parts, flos Rosae Davuricae extract 0.0006-0.012 parts, acetyl heptapeptide-40.0005-0.0015 parts, Bacillus fermentation product 0.0011-0.0033 parts, lactobacillus fermentation lysate 0.075-0.225 parts, and lactobacillus/Halloween fermentation product extract 0.98-2.94 parts.
5. The method of preparing a composition for improving skin micro-ecology according to any one of claims 1 to 4, wherein the method of preparing comprises: mixing inulin/yogurt powder mixture, flos Rosae Davuricae extract, acetyl heptapeptide-4, Bacillus fermentation product, lactobacillus fermentation lysate and lactobacillus/eriodictyon fermentation product extract.
6. The method of preparing a composition for improving skin micro-ecology according to claim 5, comprising:
(1) uniformly mixing the inulin/yoghurt powder mixture with 1, 2-pentanediol to obtain a mixture A; mixing carbomer and water, heating, keeping the temperature, and cooling to obtain solution B;
(2) mixing flos Rosae Davuricae extract, acetyl heptapeptide-4, Bacillus fermentation product, lactobacillus fermentation lysate and lactobacillus/herba Saint holtziae fermentation product extract with aminomethyl propanol, mixture A, and solution B to obtain the composition for improving skin microecology.
7. The method for preparing a composition for improving skin micro-ecology according to claim 6, wherein the step (1) of mixing inulin/yoghurt powder mixture with 1, 2-pentanediol uniformly under stirring at a speed of 100-200rpm for 1-5 min;
preferably, the heating in step (1) means heating to 80-90 ℃, preferably 84-86 ℃;
preferably, the heat preservation time in the step (1) is 20-40 min;
preferably, the temperature reduction in the step (1) is to reduce the temperature to 40-50 ℃, preferably 44-46 ℃;
preferably, the mixing in step (2) is performed under stirring at a speed of 800-1000rpm for 5-10 min.
8. Use of a composition for improving skin micro-ecology according to any one of claims 1 to 4 in the preparation of a skin care product.
9. A skin care product comprising the skin micro ecology improving composition according to any one of claims 1 to 4, wherein the skin micro ecology improving composition is contained in the skin care product in an amount of 10 to 40% by mass.
10. The skin care product of claim 9, wherein the skin care product comprises, in mass percent:
the component A comprises: 6.0-8.0% of glycerol, 1-5% of 1, 2-pentanediol, 0.18-0.2% of carbomer and the balance of water;
and B component: 10-40% of composition for improving skin microecology;
and C, component C: 0.1-12% of aminomethyl propanol.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011492057.XA CN112587471A (en) | 2020-12-16 | 2020-12-16 | Composition for improving skin micro-ecology and preparation method and application thereof |
| JP2021132124A JP7204844B2 (en) | 2020-12-16 | 2021-08-16 | Skin microecosystem-improving composition and its preparation method and use |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011492057.XA CN112587471A (en) | 2020-12-16 | 2020-12-16 | Composition for improving skin micro-ecology and preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112587471A true CN112587471A (en) | 2021-04-02 |
Family
ID=75196877
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011492057.XA Pending CN112587471A (en) | 2020-12-16 | 2020-12-16 | Composition for improving skin micro-ecology and preparation method and application thereof |
Country Status (2)
| Country | Link |
|---|---|
| JP (1) | JP7204844B2 (en) |
| CN (1) | CN112587471A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113648274A (en) * | 2021-09-07 | 2021-11-16 | 科丝美诗(中国)化妆品有限公司 | Anti-aging composition and preparation method and application thereof |
| CN114569536A (en) * | 2022-03-01 | 2022-06-03 | 山东福瑞达生物股份有限公司 | A lotion for improving microecology and physiological state of aged skin |
| CN115120551A (en) * | 2022-08-11 | 2022-09-30 | 露乐健康科技股份有限公司 | Application of chlorella extract and lactobacillus fermentation product in nursing product |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115634172B (en) * | 2022-11-03 | 2024-01-30 | 山东九鑫生物工程有限公司 | Scalp microecological stabilizer and preparation method and application thereof |
| CN115969766A (en) * | 2022-12-22 | 2023-04-18 | 上海萧雅生物科技股份有限公司 | Yeast composition and preparation method and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106361684A (en) * | 2016-08-31 | 2017-02-01 | 广东芭薇生物科技股份有限公司 | Active composition for adjusting microorganism ecological balance of skin surface |
| CN110179743A (en) * | 2019-06-20 | 2019-08-30 | 吴旭升 | A kind of composition and preparation method thereof adjusting skin microecological balance |
| CN110897940A (en) * | 2019-11-12 | 2020-03-24 | 上海妆尚生物科技有限公司 | Anti-allergy mask liquid capable of maintaining skin microbial balance and preparation method thereof |
| CN111803414A (en) * | 2020-07-13 | 2020-10-23 | 皓雨(广州)化妆品制造有限公司 | Composition for skin barrier and preparation method and application thereof |
| CN111991334A (en) * | 2020-08-26 | 2020-11-27 | 广东丸美生物技术股份有限公司 | Skin-care matrix and preparation method and application thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2889057B1 (en) | 2005-08-01 | 2008-07-18 | Oreal | COSMETIC AND / OR DERMATOLOGICAL COMPOSITION FOR THE PREVENTION AND / OR TREATMENT OF SENSITIVE OR DRY SKINS |
| JP7325170B2 (en) | 2017-02-01 | 2023-08-14 | ロート製薬株式会社 | Cosmetic composition and mitochondrial transfer promoter |
-
2020
- 2020-12-16 CN CN202011492057.XA patent/CN112587471A/en active Pending
-
2021
- 2021-08-16 JP JP2021132124A patent/JP7204844B2/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106361684A (en) * | 2016-08-31 | 2017-02-01 | 广东芭薇生物科技股份有限公司 | Active composition for adjusting microorganism ecological balance of skin surface |
| CN110179743A (en) * | 2019-06-20 | 2019-08-30 | 吴旭升 | A kind of composition and preparation method thereof adjusting skin microecological balance |
| CN110897940A (en) * | 2019-11-12 | 2020-03-24 | 上海妆尚生物科技有限公司 | Anti-allergy mask liquid capable of maintaining skin microbial balance and preparation method thereof |
| CN111803414A (en) * | 2020-07-13 | 2020-10-23 | 皓雨(广州)化妆品制造有限公司 | Composition for skin barrier and preparation method and application thereof |
| CN111991334A (en) * | 2020-08-26 | 2020-11-27 | 广东丸美生物技术股份有限公司 | Skin-care matrix and preparation method and application thereof |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113648274A (en) * | 2021-09-07 | 2021-11-16 | 科丝美诗(中国)化妆品有限公司 | Anti-aging composition and preparation method and application thereof |
| CN113648274B (en) * | 2021-09-07 | 2023-12-29 | 科丝美诗(中国)化妆品有限公司 | Anti-aging composition and preparation method and application thereof |
| CN114569536A (en) * | 2022-03-01 | 2022-06-03 | 山东福瑞达生物股份有限公司 | A lotion for improving microecology and physiological state of aged skin |
| CN114569536B (en) * | 2022-03-01 | 2023-07-28 | 山东福瑞达生物股份有限公司 | Emulsion with effect of improving microecology and physiological state of aging skin |
| CN115120551A (en) * | 2022-08-11 | 2022-09-30 | 露乐健康科技股份有限公司 | Application of chlorella extract and lactobacillus fermentation product in nursing product |
| CN115120551B (en) * | 2022-08-11 | 2024-03-22 | 露乐健康科技股份有限公司 | Chlorella extract and application of lactobacillus fermentation product in nursing products |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2022095528A (en) | 2022-06-28 |
| JP7204844B2 (en) | 2023-01-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN112587471A (en) | Composition for improving skin micro-ecology and preparation method and application thereof | |
| JP6793692B2 (en) | Uses of fermentation products in combination with thermath thermophilus and yeast | |
| TWI586373B (en) | Cosmetic composition containing biocellulose film fragments and method for manufacturing thereof | |
| CN110585114B (en) | Bactericidal composition for regulating skin microbial flora and application thereof | |
| KR101719223B1 (en) | Cosmetic composition for promoting ceramide biosynthesis containing Panax ginseng, honey, and propolis fermented by kimchi lactic acid bacteria | |
| CN113318065B (en) | Composite acid and microecological conditioning composition and preparation process thereof | |
| CN107334726A (en) | A kind of acne-removing composition and its preparation method and application | |
| CN114469843A (en) | Composition with soothing and repairing effects and preparation method and application thereof | |
| CN113413356A (en) | Peach tocol-based acne-removing composition and preparation method thereof | |
| CN114569536A (en) | A lotion for improving microecology and physiological state of aged skin | |
| CN115038425B (en) | Cosmetic composition for improving skin comprising polysaccharide, yeast extract and strain fermentation product with probiotic properties as effective components | |
| JP3184114B2 (en) | Moisturizer | |
| CN112294685A (en) | Scalp care composition with function of adjusting scalp microecological balance and preparation method and application thereof | |
| CN110339112A (en) | A kind of composition of the microbial fermentation product of the seed containing goosefoot and its application | |
| KR102353995B1 (en) | Cosmetic composition containing Nymphoides peltata and Centella asiatica fermented extract using Onyang hot spring water | |
| KR102416730B1 (en) | Preparation method of fermented vegetable oil using skin flora and cosmetic composition comprising thereof | |
| CN115400052B (en) | Carmine skin care cream and preparation method thereof | |
| KR20160001141A (en) | Antifungal Soap Using Lactic Acid Bacteria in Fermentation Process and the Method thereof | |
| KR20220114763A (en) | Multi moisturizing agent for cosmetics, Manufacturing method thereof and cosmetics containing the same | |
| KR102001241B1 (en) | Composition for improving microbial flora containing extract of Coicis Semen | |
| CN111529480A (en) | Silk fibroin skin beautifying formula and preparation method thereof | |
| KR19980080465A (en) | Cosmetic Materials and Manufacturing Method Thereof | |
| KR102643628B1 (en) | A composition for improving milium comprising lactic acid bacteria | |
| CN117695202A (en) | Probiotic metazoan composition for improving scalp environment and preparation method and application thereof | |
| KR102471166B1 (en) | Cosmetic composition |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |