KR102503339B1 - A cosmetic composition comprising a fermented product of donkey milk of complex probiotics, a functional cosmetic product including the cosmetic composition, a method for producing the fermented product and a method for producing the cosmetic composition - Google Patents
A cosmetic composition comprising a fermented product of donkey milk of complex probiotics, a functional cosmetic product including the cosmetic composition, a method for producing the fermented product and a method for producing the cosmetic composition Download PDFInfo
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- KR102503339B1 KR102503339B1 KR1020200118184A KR20200118184A KR102503339B1 KR 102503339 B1 KR102503339 B1 KR 102503339B1 KR 1020200118184 A KR1020200118184 A KR 1020200118184A KR 20200118184 A KR20200118184 A KR 20200118184A KR 102503339 B1 KR102503339 B1 KR 102503339B1
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- donkey milk
- lactobacillus
- cosmetic composition
- fermented
- donkey
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- FDDDEECHVMSUSB-UHFFFAOYSA-N sulfanilamide Chemical compound NC1=CC=C(S(N)(=O)=O)C=C1 FDDDEECHVMSUSB-UHFFFAOYSA-N 0.000 description 1
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- UEUXEKPTXMALOB-UHFFFAOYSA-J tetrasodium;2-[2-[bis(carboxylatomethyl)amino]ethyl-(carboxylatomethyl)amino]acetate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O UEUXEKPTXMALOB-UHFFFAOYSA-J 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/925—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of animal origin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/005—Preparations for sensitive skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
Abstract
Description
본 발명은, 복합 프로바이오틱스의 당나귀유 발효물을 포함하는 화장료 조성물, 이를 포함하는 기능성 화장품, 복합 프로바이오틱스의 당나귀유 발효물의 제조방법 및 이를 포함하는 화장료 조성물의 제조방법에 관한 것이다.The present invention relates to a cosmetic composition comprising a donkey milk fermented product of complex probiotics, a functional cosmetic containing the same, a method for preparing a donkey milk fermented product of a complex probiotics, and a cosmetic composition comprising the same.
피부는, 인체의 가장 큰 기관으로서 전체 인체 부피의 약 16%를 차지하며, 외부 환경과 직접 접하면서 인체 안으로 침입하려는 치명적인 많은 유해 인자, 예를 들면, 온도, 습도 및 자외선 등으로부터 인체를 보호하는 중요한 보호막 역할을 담당한다. 그러나 나이가 들어감에 따라 각종 오염 물질, 강한 자외선, 스트레스 및 영양 결핍 등으로 인해 피부 세포들이 손상을 입게 되고, 세포 증식이 제대로 이루어지지 않게 되어 피부에 주름, 탄력 손실 및 각질화 등이 발생하게 된다.Skin, as the largest organ of the human body, occupies about 16% of the total human body volume, and protects the human body from many lethal harmful factors that try to invade the human body while directly contacting the external environment, such as temperature, humidity, and ultraviolet rays. It plays an important protective role. However, with age, skin cells are damaged due to various pollutants, strong ultraviolet rays, stress, and nutritional deficiency, and cell proliferation is not performed properly, resulting in wrinkles, loss of elasticity, and keratinization of the skin.
프로바이오틱스(Probiotics)는, Pro와 Biotics의 합성어로서, Pro는 '~를 위한'을 의미하고, 'Biotics'는 생명을 뜻한다. 세계보건기구(WHO)는 프로바이오틱스를 건강에 좋은 효과를 주는 살아있는 균으로 정의하고 있으며 이 중 대표적인 것이 바로 유산균이다.Probiotics is a compound word of Pro and Biotics, Pro means 'for' and 'Biotics' means life. The World Health Organization (WHO) defines probiotics as live microorganisms that have a beneficial effect on health, and among them, lactic acid bacteria are representative.
화장품 분야에서는 유산균 배양액이 1955년 상품화된 이후 현재까지도 사용되고 있는데 주로 스트렙토코쿠스 속(Streptococcus sp.), 락토바실러스 속(Lactobacillus sp.), 락토코쿠스 속(Lactococcus sp.), 류코노스톡 속(Leuconostoc sp.), 비피도박테리움 속(Bifidobacterium sp.) 등을 이용하여 직접 배양액에 발효하여 여과하거나 추출하는 등의 원료 형태 및 활성 소재에 접종하여 발효한 후 여과하거나 추출하는 등의 연구가 활발하게 이루어지고 있다. In the field of cosmetics, lactobacillus culture medium has been commercialized in 1955 and is still being used, mainly Streptococcus sp., Lactobacillus sp., Lactococcus sp., and Leuconostoc ( Leuconostoc sp.), Bifidobacterium sp.), etc., active research on the form of raw materials such as direct fermentation in the culture medium and filtering or extraction, as well as active material inoculation and fermentation followed by filtering or extraction it is being done
종래에는 락토바실러스 속 유산균 중에서 특히 사람의 장내에 상재하는 유익균으로 알려진 락토바실러스 펜토서스(Lactobacillus pentosus)를 균주로 이용하여 유제품이나 건강기능식품, 화장품 등 다방면의 산업에서 유익하게 활용되고 있다.Conventionally, among lactic acid bacteria of the genus Lactobacillus, Lactobacillus pentosus, known as a beneficial bacterium residing in the human intestine, is used as a strain and is beneficially used in various industries such as dairy products, health functional foods, and cosmetics.
한편, 당나귀유(Donkey milk)는, 당나귀와 같이 당나귀아속에 속한 동물들에게서 나는 젖(乳)을 말한다. 당나귀유는 어미 당나귀의 출산 후 대략 6개월 동안만 착유 가능하며 착유 과정 또한 매우 까다롭기 때문에 유럽에서 화이트 골드로 불려지기도 한다.On the other hand, donkey milk (Donkey milk) refers to the milk (乳) from animals belonging to the Donkey subfamily, such as a donkey. Donkey milk can only be milked for about six months after the mother donkey gives birth, and the milking process is also very complicated, so it is sometimes called white gold in Europe.
당나귀유에는 단백질과 지방이 적고 젖당이 풍부하여 다른 포유 동물의 젖보다 인간 여성의 모유의 영양 성분에 가까운 것으로 알려져 있으며, 피부 재생 물질 내지 상피세포 성장인자로 일컬어지는 EGF(Epidemal Growth Factor) 성분이 일반 우유, 산양유, 마유에 비해 4배나 들어 있는 것으로 알려지면서 당나귀유를 화장품의 성분으로서 이용하고자 하는 관심들이 이어지고 있다.Donkey milk is low in protein and fat and rich in lactose, so it is known to have nutritional components closer to that of human female breast milk than milk of other mammals. As it is known to contain four times more than regular milk, goat milk, and horse milk, interest in using donkey milk as an ingredient in cosmetics continues.
다만, 고대 문명 시기에도 피부 미용을 위해 당나귀유를 사용한 것으로 알려져 있다. 널리 알려진 예로서, 이집트의 여왕인 클레오파트라는 피부 미용을 위해 당나귀 300여 마리를 데리고 다니며 당나귀유 목욕을 했다고 전해진다. 폼페이 전문가는 로마 사람들은 피부 관리를 위해 당나귀유를 피부 관리용으로 사용했다고 밝힌 바 있으며, 이 외에도 플라이니 더 엘더(Pliny the Elder)의 저서 자연사의 역사(The Natural History)에는 로마 황제 네로의 부인, 포페아가 당나귀유 목욕을 했다고 기록되어 있다.However, it is known that donkey milk was used for skin care even during the ancient civilization period. As a well-known example, Cleopatra, the queen of Egypt, is said to have taken a bath with donkey milk, taking around 300 donkeys for skin care. Pompeii experts have revealed that the Romans used donkey milk for skin care, and in addition to this, Pliny the Elder's book The Natural History (The Natural History), the wife of the Roman emperor Nero , it is recorded that Poppea took a bath in donkey milk.
당나귀유를 화장품 소재에 적용한 최근의 기술로서, 국내의 경우 대한민국 등록특허공보 제10-1490710호(당나귀 우유를 포함하는 화장료 조성물 및 그의 제조방법)가 있으며, 해당 등록특허공보는 당나귀로부터 획득된 당나귀우유를 저온 살균, 고온 살균 및 초고온 살균 중 어느 하나를 통해 살균하는 단계, 오리제브렌 1 내지 10 중량% 및 발효 누룩 1 내지 10 중량%를 배합하여 혼합물을 제조하는 단계, 혼합물에 살균된 당나귀유 0.1 내지 10 중량%, 정제수 또는 증류수 20 내지 80 중량% 및 오일 15 내지 70 중량%를 배합하여 반죽물을 제조하는 단계 및 반죽물을 10 ~ 15℃의 저온 냉장 창고 또는 상온에서 7 ~ 10일 동안 숙성시키는 단계를 포함하는 것을 특징으로 하는 화장료 조성물의 제조 방법을 개시한다.As a recent technology that applies donkey milk to cosmetic materials, in Korea, there is Korean Patent Registration No. 10-1490710 (Cosmetic Composition Containing Donkey Milk and Manufacturing Method), Pasteurizing milk through any one of pasteurization, high-temperature pasteurization, and ultra-high-temperature pasteurization, preparing a mixture by blending 1 to 10% by weight of orizebrene and 1 to 10% by weight of fermented yeast, sterilized donkey milk in the mixture Preparing a dough by blending 0.1 to 10% by weight, 20 to 80% by weight of purified water or distilled water and 15 to 70% by weight of oil, and preparing the dough at a low temperature refrigerated warehouse at 10 to 15 ° C. or at room temperature for 7 to 10 days Disclosed is a method for producing a cosmetic composition comprising the step of aging.
본 발명은, 복합 프로바이오틱스의 당나귀유 발효물을 포함하는 화장료 조성물, 보다 구체적으로 복합 프로바이오틱스의 당나귀유 대사 산물을 유효성분으로 포함하는 화장료 조성물을 제공하여 피부 각질대사 활성화, 염증 완화 및 특히, 미백 효능을 향상시키는 것을 목적으로 한다.The present invention provides a cosmetic composition comprising a donkey milk fermentation product of complex probiotics, more specifically, a cosmetic composition comprising donkey oil metabolites of complex probiotics as an active ingredient, thereby activating skin keratin metabolism, mitigating inflammation and, in particular, whitening effect. is aimed at improving
본 발명은, 상기 화장료 조성물을 포함하는 기능성 화장품을 제공하여 피부 각질대사 활성화, 염증 완화 및 특히, 미백 효능을 향상시키는 것을 목적으로 한다.An object of the present invention is to provide functional cosmetics containing the cosmetic composition to activate skin keratin metabolism, relieve inflammation and, in particular, improve whitening efficacy.
본 발명은, 상기 복합 프로바이오틱스의 당나귀유 발효물의 제조방법을 제공하는 것을 목적으로 한다.An object of the present invention is to provide a method for producing a donkey milk fermented product of the complex probiotics.
본 발명은, 상기 화장료 조성물의 제조방법을 제공하는 것을 목적으로 한다.An object of the present invention is to provide a method for producing the cosmetic composition.
다만, 본 발명의 목적들은 상기에 언급된 목적으로 제한되지 않으며, 언급되지 않은 또 다른 목적들은 아래의 기재로부터 당업자에게 명확하게 이해될 수 있을 것이다.However, the objects of the present invention are not limited to the above-mentioned objects, and other objects not mentioned will be clearly understood by those skilled in the art from the description below.
본 발명은, 화장료 조성물로서, 락토바실러스 사케이(Lactobacillus sakei), 락토바실러스 브레비스(Lactobacillus brevis), 락토바실러스 락티스(Lactobacillus lactis), 락토바실러스 카제이(Lactobacillus casei) 및 락토바실러스 아시도필루스(Lactobacillus acidophilus)로 구성되는 군에서 선택되는 4종 이상의 균을 포함하는 복합 프로바이오틱스의 당나귀유 발효물을 유효성분으로 포함하는 것을 특징으로 한다.The present invention, as a cosmetic composition, Lactobacillus sakei, Lactobacillus brevis, Lactobacillus lactis, Lactobacillus casei and Lactobacillus acidophilus ( Lactobacillus acidophilus) is characterized in that it contains the fermented donkey milk of complex probiotics containing 4 or more species of bacteria selected from the group consisting of as an active ingredient.
또한, 상기 락토바실러스 사케이(Lactobacillus sakei)의 당나귀유 발효물은 상기 복합 프로바이오틱스의 당나귀유 발효물 전체 중량에 대하여 1 내지 15 중량%로 포함되는 것을 특징으로 한다.In addition, the fermented donkey milk of Lactobacillus sakei is characterized in that it is included in 1 to 15% by weight based on the total weight of the fermented donkey milk of the complex probiotics.
또한, 상기 락토바실러스 브레비스(Lactobacillus brevis)의 당나귀유 발효물은 상기 복합 프로바이오틱스의 당나귀유 발효물 전체 중량에 대하여 5 내지 25 중량%로 포함되는 것을 특징으로 한다.In addition, the fermented donkey milk of Lactobacillus brevis is characterized in that it is included in 5 to 25% by weight based on the total weight of the fermented donkey milk of the complex probiotics.
또한, 상기 락토바실러스 락티스(Lactobacillus lactis)의 당나귀유 발효물은 상기 복합 프로바이오틱스의 당나귀유 발효물 전체 중량에 대하여 20 내지 40 중량%로 포함되는 것을 특징으로 한다.In addition, the fermented donkey milk of Lactobacillus lactis is characterized in that it is included in 20 to 40% by weight based on the total weight of the fermented donkey milk of the complex probiotics.
또한, 상기 락토바실러스 카제이(Lactobacillus casei)의 당나귀유 발효물은 상기 복합 프로바이오틱스의 당나귀유 발효물 전체 중량에 대하여 15 내지 35 중량%로 포함되는 것을 특징으로 한다.In addition, the fermented donkey milk of Lactobacillus casei is characterized in that it is included in 15 to 35% by weight based on the total weight of the fermented donkey milk of the complex probiotics.
또한, 상기 락토바실러스 아시도필루스(Lactobacillus acidophilus)의 당나귀유 발효물은 상기 복합 프로바이오틱스의 당나귀유 발효물 전체 중량에 대하여 5 내지 25 중량%로 포함되는 것을 특징으로 한다.In addition, the fermented donkey milk of Lactobacillus acidophilus is characterized in that it is included in 5 to 25% by weight based on the total weight of the fermented donkey milk of the complex probiotics.
본 발명은, 상기 화장료 조성물을 포함하는 기능성 화장픔으로서, 미백 및 트러블케어 가능한 것을 특징으로 한다.The present invention, as a functional cosmetic comprising the cosmetic composition, is characterized in that it is capable of whitening and trouble care.
본 발명은, 복합 프로바이오틱스의 당나귀유 발효물 제조방법으로서, 당나귀유 분말 수용액을 제조하여 당나귀유 배지를 형성하는 배지제조단계(S10) 및 락토바실러스 사케이(Lactobacillus sakei), 락토바실러스 브레비스(Lactobacillus brevis), 락토바실러스 락티스(Lactobacillus lactis), 락토바실러스 카제이(Lactobacillus casei) 및 락토바실러스 아시도필루스(Lactobacillus acidophilus)로 구성되는 군에서 선택되는 4종 이상의 균을 상기 당나귀유 배지에 각각 접종한 후 배양하는 배양단계(S20)를 포함하는 것을 특징으로 한다.The present invention is a method for producing a donkey milk fermented product of complex probiotics, comprising a medium preparation step (S10) of preparing an aqueous solution of donkey milk powder to form a donkey milk medium, and Lactobacillus sakei, Lactobacillus brevis ), Lactobacillus lactis, Lactobacillus casei, and Lactobacillus acidophilus are inoculated into the donkey milk medium, respectively. It is characterized in that it comprises a culturing step (S20) of culturing after.
또한, 상기 배지제조단계(S10)는 당나귀유 분말을 이용하여 10 내지 40 g/L 의 당나귀유 분말 수용액을 제조하고 멸균하여 당나귀유 배지를 제조하는 단계인 것을 특징으로 한다.In addition, the medium preparation step (S10) is characterized in that a donkey milk medium is prepared by preparing an aqueous solution of 10 to 40 g/L of donkey milk powder using donkey milk powder and sterilizing it.
또한, 상기 배양단계(S20)는 상기 4종 이상의 균을 상기 당나귀유 배지에 5 내지 10%로 각각 접종하고 30 내지 40℃의 혐기 조건에서 24 내지 36 시간 배양하는 단계인 것을 특징으로 한다.In addition, the culturing step (S20) is characterized in that the four or more bacteria are inoculated into the donkey milk medium at 5 to 10%, respectively, and cultured for 24 to 36 hours under anaerobic conditions at 30 to 40 ° C.
본 발명은, 화장료 조성물의 제조방법으로서, 당나귀유 분말 수용액을 제조하여 당나귀유 배지를 형성하는 배지제조단계(S10), 락토바실러스 사케이(Lactobacillus sakei), 락토바실러스 브레비스(Lactobacillus brevis), 락토바실러스 락티스(Lactobacillus lactis), 락토바실러스 카제이(Lactobacillus casei) 및 락토바실러스 아시도필루스(Lactobacillus acidophilus)로 구성되는 군에서 선택되는 4종 이상의 균을 상기 당나귀유 배지에 각각 접종한 후 배양하는 배양단계(S20), 상기 각각 배양된 당나귀유 배양액을 원심분리 후 여과하여 균주를 제거하고 당나귀유 발효물을 얻는 정제단계(S30) 및 상기 얻어진 각각의 당나귀유 발효물을 혼합하는 혼합단계(S40)를 포함하는 것을 특징으로 한다.The present invention is a method for producing a cosmetic composition, comprising a medium preparation step (S10) of preparing an aqueous solution of donkey milk powder to form a donkey oil medium, Lactobacillus sakei, Lactobacillus brevis, Lactobacillus Culture in which four or more bacteria selected from the group consisting of Lactobacillus lactis, Lactobacillus casei, and Lactobacillus acidophilus are inoculated into the donkey milk medium and then cultured Step (S20), a purification step (S30) of centrifuging and filtering each of the cultured donkey milk culture broths to remove strains and obtaining a fermented donkey milk product (S30), and a mixing step (S40) of mixing each of the obtained fermented donkey milk products It is characterized in that it includes.
본 발명은, 복합 프로바이오틱스의 당나귀유 발효물을 포함하는 화장료 조성물, 보다 구체적으로 복합 프로바이오틱스의 당나귀유 대사 산물을 유효성분으로 포함하는 화장료 조성물을 제공하여 피부 각질대사 활성화, 염증 완화 및 특히, 미백 효능을 향상시킬 수 있다.The present invention provides a cosmetic composition comprising a donkey milk fermentation product of complex probiotics, more specifically, a cosmetic composition comprising donkey oil metabolites of complex probiotics as an active ingredient, thereby activating skin keratin metabolism, mitigating inflammation and, in particular, whitening effect. can improve
본 발명은, 상기 화장료 조성물을 포함하는 기능성 화장품을 제공하여 피부 각질대사 활성화, 염증 완화 및 특히, 미백 효능을 향상시킬 수 있다.The present invention, by providing a functional cosmetic comprising the cosmetic composition, it is possible to activate skin keratin metabolism, relieve inflammation and, in particular, improve whitening efficacy.
본 발명은, 상기 복합 프로바이오틱스의 당나귀유 발효물의 제조방법을 제공할 수 있다.The present invention can provide a method for preparing the donkey milk fermented product of the complex probiotics.
본 발명은, 상기 화장료 조성물의 제조방법을 제공할 수 있다.The present invention may provide a method for preparing the cosmetic composition.
이하에 본 발명을 상세하게 설명하기에 앞서, 본 명세서에 사용된 용어는 특정의 실시예를 기술하기 위한 것일 뿐 첨부하는 특허청구의 범위에 의해서만 한정되는 본 발명의 범위를 한정하려는 것은 아님을 이해하여야 한다. 본 명세서에 사용되는 모든 기술용어 및 과학용어는 다른 언급이 없는 한은 기술적으로 통상의 기술을 가진 자에게 일반적으로 이해되는 것과 동일한 의미를 가진다.Prior to describing the present invention in detail below, it is understood that the terms used herein are intended to describe specific embodiments and are not intended to limit the scope of the present invention, which is limited only by the appended claims. shall. All technical and scientific terms used herein have the same meaning as commonly understood by a person of ordinary skill in the art unless otherwise specified.
본 명세서 및 청구범위의 전반에 걸쳐, 다른 언급이 없는 한 포함(comprise, comprises, comprising)이라는 용어는 언급된 물건, 단계 또는 일군의 물건, 및 단계를 포함하는 것을 의미하고, 임의의 어떤 다른 물건, 단계 또는 일군의 물건 또는 일군의 단계를 배제하는 의미로 사용된 것은 아니다.Throughout this specification and claims, the terms "comprise", "comprise" and "comprising", unless stated otherwise, are meant to include a stated object, step or group of objects, and steps, and any other object However, it is not used in the sense of excluding a step or a group of objects or a group of steps.
한편, 본 발명의 여러 가지 실시예들은 명확한 반대의 지적이 없는 한 그 외의 어떤 다른 실시예들과 결합될 수 있다. 특히 바람직하거나 유리하다고 지시하는 어떤 특징도 바람직하거나 유리하다고 지시한 그 외의 어떤 특징 및 특징들과 결합될 수 있다.On the other hand, various embodiments of the present invention can be combined with any other embodiments unless clearly indicated to the contrary. Any feature indicated as being particularly desirable or advantageous may be combined with any other features and characteristics indicated as being particularly desirable or advantageous.
본 발명의 일 실시예에 따른 화장료 조성물은, 복합 프로바이오틱스의 당나귀유 발효물을 포함하여 보다 구체적으로, 복합 프로바이오틱스의 당나귀유 발효 대사 산물을 유효성분으로 포함함으로써 피부에 지속적으로 도포할 경우 각질대사 활성화, 멜라닌 생성 억제를 통한 미백 효과, 항균 및 피지제거를 통한 트러블케어 효과를 제공할 수 있다. 또한, 상기 복합 프로바이오틱스의 당나귀유 발효물을 포함하는 화장료 조성물은 미백 및 트러블 케어 기능성 화장품에 사용될 수 있다.The cosmetic composition according to an embodiment of the present invention includes, as an active ingredient, a fermentation product of donkey milk of complex probiotics, and more specifically, a metabolite of fermentation of donkey milk of complex probiotics, so that keratin metabolism is activated when continuously applied to the skin. , It can provide a whitening effect through inhibition of melanin production, and a trouble care effect through antibacterial and sebum removal. In addition, the cosmetic composition containing the donkey milk fermented product of the complex probiotics can be used for whitening and trouble care functional cosmetics.
본 명세서에서, 상기 "유효성분"의 의미는 단독으로 목적하는 활성을 나타내거나 또는 그 자체는 활성이 없는 담체와 함께 활성을 나타낼 수 있는 성분을 의미한다. 기타 본 명세서에서 특별히 정의되지 아니한 용어에 대해서는 국어사전적 의미나 당업계에서 통상 받아들여지고 있는 의미를 따른다.In the present specification, the meaning of the "active ingredient" means a component that exhibits the desired activity alone or can exhibit activity in combination with a carrier having no activity itself. For other terms not specifically defined in this specification, the meaning of the Korean dictionary or the meaning commonly accepted in the art follows.
본 발명의 일 실시예에 따른 화장료 조성물은, 락토바실러스 사케이(Lactobacillus sakei), 락토바실러스 브레비스(Lactobacillus brevis), 락토바실러스 락티스(Lactobacillus lactis), 락토바실러스 카제이(Lactobacillus casei) 및 락토바실러스 아시도필루스(Lactobacillus acidophilus)로 구성되는 군에서 선택되는 어느 4종 이상, 바람직하게는 5종 모두를 포함하는 균의 당나귀유 발효물을 포함한다.The cosmetic composition according to an embodiment of the present invention includes Lactobacillus sakei, Lactobacillus brevis, Lactobacillus lactis, Lactobacillus casei and Lactobacillus asia It includes a fermented product of donkey's milk of a bacterium containing any 4 or more species selected from the group consisting of Lactobacillus acidophilus, preferably all 5 species.
상기 락토바실러스 사케이(Lactobacillus sakei)는, 혈당 개선, 지방세포 분화 억제, 체지방 감소 효능이 있고 락토바실러스 사케이(Lactobacillus sakei)의 당나귀유 발효물이 화장료 조성물에 포함되어 민감성 피부에 풍분한 수분감을 주어 피부 상태를 호전시키고, 미백 효과 및 주름 개선 효과를 제공할 수 있다. The Lactobacillus sakei has blood sugar improvement, fat cell differentiation inhibition, and body fat reduction effects, and the donkey milk fermentation product of Lactobacillus sakei is included in the cosmetic composition to provide rich moisture to sensitive skin. It can improve the condition of the skin and provide a whitening effect and wrinkle improvement effect.
상기 락토바실러스 사케이(Lactobacillus sakei)의 당나귀유 발효물은 화장료 조성물 전체 중량에 대하여 1 내지 15 중량%로 포함된다. 바람직하게는 5 내지 10 중량%로 포함되는 것이 좋다. 더욱 바람직하게는 8 중량%로 포함되는 것이 좋다.The donkey milk fermented product of Lactobacillus sakei is included in an amount of 1 to 15% by weight based on the total weight of the cosmetic composition. It is preferably included in 5 to 10% by weight. more preferably 8 It is preferable to include it in weight percent.
상기 락토바실러스 브레비스(Lactobacillus brevis)는, 면역 기관에 작용하여 항바이러스 단백질을 만들도록 하고 락토바실러스 브레비스(Lactobacillus brevis)의 당나귀유 발효물이 화장료 조성물에 포함되면 염증성질환에 효과적이어서 피부 진정 및 트러블 개선 효과를 제공할 수 있다. The Lactobacillus brevis acts on the immune system to make antiviral proteins, and when the donkey milk fermentation product of Lactobacillus brevis is included in a cosmetic composition, it is effective in inflammatory diseases, soothes skin and improves troubles. effect can be provided.
상기 락토바실러스 브레비스(Lactobacillus brevis)의 당나귀유 발효물은 화장료 조성물 전체 중량에 대하여 5 내지 25 중량%로 포함된다. 바람직하게는 10 내지 20 중량%로 포함되는 것이 좋다. 더욱 바람직하게는 16 중량%로 포함되는 것이 좋다.The donkey milk fermented product of Lactobacillus brevis is included in an amount of 5 to 25% by weight based on the total weight of the cosmetic composition. It is preferably included in 10 to 20% by weight. More preferably, it is included in 16% by weight.
상기 락토바실러스 락티스(Lactobacillus lactis)는, 면역 관련 물질의 생산을 자극하는 효능이 있어 락토바실러스 락티스(Lactobacillus lactis)의 당나귀유 발효물이 화장료 조성물에 포함되어 자외선과 외부환경에 의해 손상 받은 피부에 대하여 염증완화면역 과민 반응에 의한 피부 상태를 개선하고 피부 각질대사를 활성화하여 미백 효과를 증대시킬 수 있다.The Lactobacillus lactis has an effect of stimulating the production of immune-related substances, so the fermentation of donkey milk of Lactobacillus lactis is included in a cosmetic composition to treat skin damaged by ultraviolet rays and the external environment. It can improve the skin condition caused by hypersensitivity reaction to relieve inflammation and increase the whitening effect by activating skin keratin metabolism.
상기 락토바실러스 락티스(Lactobacillus lactis)의 당나귀유 발효물은 화장료 조성물 전체 중량에 대하여 20 내지 40 중량%로 포함된다. 바람직하게는 25 내지 35 중량%로 포함되는 것이 좋다. 더욱 바람직하게는 33 중량%로 포함되는 것이 좋다.The donkey milk fermented product of Lactobacillus lactis is included in an amount of 20 to 40% by weight based on the total weight of the cosmetic composition. It is preferably included in 25 to 35% by weight. More preferably, it is good to be included in 33% by weight.
상기 락토바실러스 카제이(Lactobacillus casei)는 면역조절능력, 유해균 억제 및 정장작용의 효능이 있어 락토바실러스 카제이(Lactobacillus casei)의 당나귀유 발효물이 화장료 조성물에 포함되어 피부 진정, 알레르기 피부 개선 및 피부 유연 효과를 제공할 수 있다. The Lactobacillus casei has immunomodulatory ability, suppression of harmful bacteria, and intestinal function, so that the fermented donkey milk of Lactobacillus casei is included in a cosmetic composition to soothe skin, improve allergic skin, and improve skin It can provide a softening effect.
상기 락토바실러스 카제이(Lactobacillus casei)의 당나귀유 발효물은 화장료 조성물 전체 중량에 대하여 15 내지 35 중량%로 포함된다. 바람직하게는 20 내지 30 중량%로 포함되는 것이 좋다. 더욱 바람직하게는 27 중량%로 포함되는 것이 좋다.The donkey milk fermented product of Lactobacillus casei is included in an amount of 15 to 35% by weight based on the total weight of the cosmetic composition. It is preferably included in 20 to 30% by weight. More preferably, it is good to be included in 27% by weight.
상기 락토바실러스 아시도필루스(Lactobacillus acidophilus)는 생존 능력이 우수한 균으로서, 면역조절능력, 콜레스테롤 제거, 아토피 치료, 살모넬라균 억제, 헬리코박터 파이로리균 제거 효능이 있어 락토바실러스 아시도필루스(Lactobacillus acidophilus)의 당나귀유 발효물이 화장료 조성물에 포함되어 알레르기 피부 개선 및 주름 개선 효과를 제공할 수 있다.The Lactobacillus acidophilus is a bacterium with excellent viability, and has immunomodulatory ability, cholesterol removal, atopic treatment, salmonella suppression, and Helicobacter pylori removal effect. Lactobacillus acidophilus A fermented donkey milk of is included in a cosmetic composition to provide allergic skin and wrinkle-reducing effects.
상기 락토바실러스 아시도필루스(Lactobacillus acidophilus)의 당나귀유 발효물은 화장료 조성물 전체 중량에 대하여 5 내지 25 중량%로 포함된다. 바람직하게는 10 내지 20 중량%로 포함되는 것이 좋다. 더욱 바람직하게는 16 중량%로 포함되는 것이 좋다.The donkey milk fermented product of Lactobacillus acidophilus is included in an amount of 5 to 25% by weight based on the total weight of the cosmetic composition. It is preferably included in 10 to 20% by weight. More preferably, it is good to include 16% by weight.
본 발명의 일 실시예에 따른 화장료 조성물을 포함하는 기능성 화장품은, 복합 프로바이오틱스의 당나귀유 대사 산물을 유효성분으로 포함함으로써 각질대사 활성화 및 멜라닌 생성 억제를 통한 미백 효과, 항균 및 피지제거를 통한 트러블케어 효과를 제공할 수 있다.Functional cosmetics including the cosmetic composition according to an embodiment of the present invention contain donkey oil metabolites of complex probiotics as an active ingredient, thereby activating keratin metabolism and inhibiting melanin production, thereby providing whitening effect, antibacterial activity, and trouble care through sebum removal. effect can be provided.
기능성 화장품으로는 상기 화장료 조성물을 포함하는 스킨 및 바디 케어 화장품으로서 에센스, 앰플, 비누, 토닉 및 바디 에센스, 에멀젼, 로션, 크림(수중유적형, 유중수적형, 다중상), 용액, 현탁액(무수 및 수계), 무수 생성물(오일 및 글리콜계), 젤, 분말 등의 다양한 제형으로 제조될 수 있으며, 상기 화장료 조성물은 전체 기능성 화장품에 대하여 0.05 내지 100 wt%로 포함되고, 앰플 제형의 경우 상기 화장료 조성물 100%로 이루어진 것일 수 있다.Functional cosmetics include essence, ampoule, soap, tonic and body essence, emulsion, lotion, cream (oil-in-water type, water-in-oil type, multi-phase), solution, suspension (anhydrous) as skin and body care cosmetics containing the cosmetic composition. And water-based), anhydrous products (oil and glycol-based), can be prepared in various formulations such as gel, powder, etc., the cosmetic composition is included in 0.05 to 100 wt% with respect to the total functional cosmetics, in the case of an ampoule formulation, the cosmetic It may consist of 100% of the composition.
화장료 조성물은 복합 프로바이오틱스의 당나귀유 발효물 이외에 화장품 제제에 있어서 수용가능한 담체를 포함할 수 있다. 상기 담체로서는 알콜, 오일, 계면활성제, 지방산, 실리콘 오일, 방부제, 습윤제, 보습제, 점성 변형제, 유제, 안정제, 자외선 차단제, 발색제, 향료, 희석제 등이 예시될 수 있다.The cosmetic composition may include carriers acceptable in cosmetic formulations in addition to the fermented donkey milk complex probiotics. Alcohols, oils, surfactants, fatty acids, silicone oils, preservatives, humectants, humectants, viscosity modifiers, emulsions, stabilizers, sunscreens, coloring agents, fragrances, diluents, and the like may be exemplified as the carrier.
상기 알코올, 오일, 계면활성제, 지방산, 실리콘 오일, 방부제, 습윤제, 보습제, 점성 변형제, 유제, 안정제, 자외선 차단제, 발색제, 향료, 희석제 등으로 사용될 수 있는 구체적인 화합물 또는 조성물은 이미 당업계에 공지되어 있기 때문에 당업자라면 적절한 해당 화합물 또는 조성물을 선택하여 사용할 수 있다.Specific compounds or compositions that can be used as the alcohol, oil, surfactant, fatty acid, silicone oil, preservative, humectant, humectant, viscosity modifier, emulsion, stabilizer, sunscreen, coloring agent, fragrance, diluent, etc. are already known in the art. Therefore, those skilled in the art can select and use an appropriate compound or composition.
여기서 몇 가지를 예시하여 보면, 알콜로서는 고급 알콜, 프로필렌 글리콜, 1,3-부틸렌 글리콜, 글리세린, 소르비톨, 폴리에틸렌글리콜 등의 수용성 다가 알콜 등을 들 수 있고, 오일로서는 아보가드유, 팜유, 우지, 호호바유 등을 들 수 있고, 방부제로서는 에틸파라벤, 부틸파라벤 등을 들 수 있으며, 보습제로서는 히알론산, 황산콘드로이친(chondroitin sulfate), 피롤리돈카복실산염 등을 들 수 있으며, 희석제로서는 에탄올, 이소프로판올 등을 들 수 있다.Here, to give some examples, alcohols include higher alcohols, propylene glycol, 1,3-butylene glycol, glycerin, sorbitol, and water-soluble polyhydric alcohols such as polyethylene glycol. Examples of oils include avogard oil, palm oil, beef tallow, jojoba oil, etc., examples of preservatives include ethyl paraben, butyl paraben, etc.; moisturizing agents include hyaluronic acid, chondroitin sulfate, pyrrolidone carboxylate, etc.; diluents include ethanol, isopropanol, etc. can be heard
더욱 구체적으로 화장품의 제형이 페이스트, 크림 또는 겔인 경우에는 담체 성분으로서 동물섬유, 식물섬유, 왁스, 파라핀, 전분, 트라칸트, 셀룰로오스 유도체, 폴리에틸렌 글리콜, 실리콘, 벤토나이트, 실리카, 탈크 또는 산화아연 등이 이용될 수 있다.More specifically, when the formulation of a cosmetic is a paste, cream or gel, animal fiber, vegetable fiber, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide as a carrier component can be used
또한 제형이 파우더 또는 스프레이인 경우에는 담체 성분으로서 락토스, 탈크, 실리카, 알루미늄 히드록시드, 칼슘 실리케이트 또는 폴리아미드 파우더가 이용될 수 있고, 특히 스프레이인 경우에는 추가적으로 클로로플루오로히드로카본, 프로판/부탄 또는 디메틸 에테르와 같은 추진체를 포함할 수 있다.In addition, when the formulation is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, especially in the case of a spray, additionally chlorofluorohydrocarbon, propane / butane or a propellant such as dimethyl ether.
또한 제형이 용액 또는 유탁액의 경우에는 담체 성분으로서 용매, 용매화제 또는 유탁화제가 이용되고, 예컨대 물, 에탄올, 이소프로판올, 에틸 카보네이트, 에틸 아세테이트, 벤질 알코올, 벤질 벤조에이트, 프로필렌 글리콜, 1,3-부틸글리콜 오일, 글리세롤 지방족 에스테르, 폴리에틸렌 글리콜 또는 소르비탄의 지방산에스테르가 있다.In addition, when the formulation is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3 -Butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan.
또한 제형이 현탁액인 경우에는 담체 성분으로서 물, 에탄올 또는 프로필렌 글리콜과 같은 액상 희석제, 에톡실화 이소스테아릴 알코올, 폴리옥시에틸렌 소르비톨 에스테르 및 폴리옥시에틸렌 소르비탄 에스테르와 같은 현탁제, 미소결정성 셀룰로오스, 알루미늄 메타히드록시드, 벤토나이트, 아가 또는 트라칸트 등이 이용될 수 있다.In addition, when the formulation is a suspension, water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline cellulose, Aluminum metahydroxide, bentonite, agar or tracanth and the like may be used.
또한 제형이 계면-활성제 함유 클린징인 경우에는 담체 성분으로서 지방족 알코올 설페이트, 지방족 알코올 에테르 설페이트, 설포숙신산 모노에스테르, 이세티오네이트, 이미다졸리늄 유도체, 메틸타우레이트, 사르코시네이트, 지방산 아미드 에테르 설페이트, 알킬아미도베타인, 지방족 알코올, 지방산 글리세리드, 지방산 디에탄올아미드, 식물성 유, 리놀린 유도체 또는 에톡실화 글리세롤 지방산 에스테르 등이 이용될 수 있다In addition, when the formulation is surfactant-containing cleansing, as carrier components, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether sulfate , alkylamidobetaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolin derivatives or ethoxylated glycerol fatty acid esters, etc. may be used.
또한 본 발명의 일 실시예에 따른 화장료 조성물을 포함하는 비누의 경우, 비누 기재에 화장료 조성물을 포함하여 제조될 수 있으며, 첨가제로서 피부 보습제, 유화제, 경수 연화제 등을 포함하여 제조될 수 있다.In addition, in the case of a soap containing the cosmetic composition according to an embodiment of the present invention, it may be prepared by including the cosmetic composition in a soap base, and may be prepared by including a skin moisturizer, an emulsifier, a water softener, and the like as additives.
상기 비누 기재로서는 야자유, 팜유, 대두유, 파마자유, 올리브유, 팜핵류 등의 식물유지 또는 우지, 돈지, 양지, 어유 등의 동물유지 등이 사용될 수 있고, 상기 피부 보습제로서는 글리세린, 에리트리톨, 폴리에틸렌글리콜, 프로필렌글리콜, 부틸렌글리콜, 펜틸렌글리콜, 헥실글리콜, 이소프로필미리스테이트, 실리콘 유도체, 알로에베라, 솔비톨 등이 사용될 수 있으며, 상기 유화제로서는 천연오일, 왁스 지방알콜, 탄화수소류, 천연식물 추출물 등이 사용될 수 있고, 상기 경수연화제로서는 테트라소듐 이디티에이 등이 사용될 수 있다.Vegetable oils such as palm oil, palm oil, soybean oil, castor oil, olive oil, and palm kernels, or animal oils such as beef tallow, lard, brisket, and fish oil may be used as the soap base material, and as the skin moisturizer, glycerin, erythritol, polyethylene glycol , Propylene glycol, butylene glycol, pentylene glycol, hexyl glycol, isopropyl myristate, silicone derivatives, aloe vera, sorbitol, etc. may be used, and natural oils, waxy fatty alcohols, hydrocarbons, natural plant extracts, etc. may be used, and tetrasodium EDTA may be used as the water softening agent.
본 발명의 비누 조성물은 또한 첨가제로서 항균제, 분산제, 거품억제제, 용매, 물때 방지제, 부식 방지제, 향료, 색소, 금속이온 봉쇄제, 산화방지제, 방부제 등을 추가적으로 포함할 수 있다.The soap composition of the present invention may further include an antibacterial agent, a dispersing agent, a foam inhibitor, a solvent, an antiscaling agent, a corrosion inhibitor, a flavoring agent, a coloring agent, a metal ion sequestering agent, an antioxidant, a preservative, and the like as additives.
본 발명의 일 실시예에 따른 화장료 조성물에 포함되는 복합 프로바이오틱스의 당나귀유 발효물의 제조방법은 당나귀유 분말 수용액을 제조하여 당나귀유 배지를 형성하는 배지제조단계(S10), 락토바실러스 사케이(Lactobacillus sakei), 락토바실러스 브레비스(Lactobacillus brevis), 락토바실러스 락티스(Lactobacillus lactis), 락토바실러스 카제이(Lactobacillus casei) 및 락토바실러스 아시도필루스(Lactobacillus acidophilus)로 구성되는 군에서 선택되는 4종 이상의 균을 각각 상기 당나귀유 배지에 접종 후 배양하는 배양단계(S20) 및 원심분리 후 여과하여 균주를 제거하고 당나귀유 발효물을 얻는 정제단계(S30)를 포함한다. According to an embodiment of the present invention, the method for producing a donkey milk fermented product of complex probiotics included in a cosmetic composition includes a medium preparation step (S10) of preparing an aqueous donkey milk powder solution to form a donkey milk medium, Lactobacillus sakei (Lactobacillus sakei) ), Lactobacillus brevis, Lactobacillus lactis, Lactobacillus casei, and Lactobacillus acidophilus. Each includes a culture step of inoculating and culturing the donkey milk medium (S20) and a purification step (S30) of obtaining a fermented donkey milk product by filtering to remove strains after centrifugation.
상기 배지제조단계(S10)는 당나귀유 분말을 이용하여 10 내지 40 g/L 의 당나귀유 분말 수용액을 제조하고 멸균하여 당나귀유 배지를 제조하는 단계이다. 저농도로 제조하는 경우 10 내지 20 g/L 의 당나귀유 분말 수용액을 제조하고, 고농도로 제조하는 경우 30 내지 40 g/L 의 당나귀유 분말 수용액을 제조한다. 바람직하게는 저농도로 제조하는 것이 좋다. The medium preparation step (S10) is a step of preparing a donkey milk medium by preparing an aqueous solution of 10 to 40 g/L of donkey milk powder using donkey milk powder and sterilizing it. A 10 to 20 g/L aqueous solution of donkey milk powder is prepared when prepared at a low concentration, and an aqueous solution of 30 to 40 g/L donkey milk powder is prepared when prepared at a high concentration. Preferably, it is good to prepare at a low concentration.
상기 배양단계(S20)는 상기 4종 이상의 균을 각각 상기 제조된 당나귀유 배지에 각각 접종 후 배양하는 단계로서, 상기 당나귀유 배지에 5 내지 10% 접종하고 30 내지 40℃의 혐기 조건에서 24 내지 36 시간 배양하여 상기 균주들의 당나귀유 발효 대사 산물을 생성하는 단계이다. 상기 복합 균주의 당나귀유 발효 대사 산물로서 젖산(Lactic acid) 등을 포함하고, 젖산은 방부제, 각질제거제, 보습제 등의 제품에 산성을 제공하여 각질층의 수분 결합 능력을 증가시킬 수 있다. The culturing step (S20) is a step of inoculating and culturing the four or more kinds of bacteria in the prepared donkey milk medium, respectively, inoculating 5 to 10% of the donkey milk medium and incubating 24 to 10% in anaerobic conditions at 30 to 40 ° C. This is a step of culturing for 36 hours to produce metabolites of donkey milk fermentation of the above strains. Lactic acid is included as a metabolite of donkey milk fermentation of the complex strain, and lactic acid provides acidity to products such as preservatives, exfoliants, and moisturizers, thereby increasing the water-binding ability of the stratum corneum.
상기 정제단계(S30)는 상기 각각 배양된 당나귀유 배양액을 원심분리 후 여과하여 균주를 제거하고 당나귀유 발효물을 얻는 단계로서, 상기 당나귀유 배양액을 5000 내지 6000rpm에서 5 내지 15분 동안 원심분리한 후 상등액을 여과하여 그 여액(filtrate)을 당나귀유 발효물로서 얻는다. The purification step (S30) is a step of centrifuging and filtering the cultured donkey milk culture to remove strains and obtaining a fermentation product of donkey milk. Afterwards, the supernatant is filtered to obtain the filtrate as a fermentation product of donkey milk.
본 발명의 일 실시예에 따른 화장료 조성물은 상기 제조된 복합 프로바이오틱스의 당나귀유 발효물을 상기의 비율로 혼합하는 혼합단계(S40)를 포함하여 제조할 수 있다. The cosmetic composition according to an embodiment of the present invention may be prepared by including a mixing step (S40) of mixing the donkey milk fermented product of the prepared complex probiotics in the above ratio.
본 발명의 일 실시예에 따른 기능성 화장품은 상기 제조된 화장료 조성물을 기타 화장품을 구성하는 성분과 혼합하여 원하는 제형의 화장품으로 제조하는 제형화단계(S50)를 포함하여 제조된다.Functional cosmetics according to an embodiment of the present invention is prepared by mixing the cosmetic composition prepared above with other ingredients constituting cosmetics to prepare a cosmetic of a desired formulation (S50).
본 발명의 일 실시예에 따른 제형화단계(S50)는 상기 제조된 기능성 조성물 및 기타 화장품 성분을 혼합하여 에센스, 로션, 크림, 앰플 등의 화장품으로 제조하는 단계이다. 또한 상기 얻어진 식물의 발효추출물을 멸균필터(0.45 내지 0.2μm)로 멸균처리한 것을 사용하여 제형화될 수 있다. Formulation step (S50) according to an embodiment of the present invention is a step of preparing cosmetics such as essence, lotion, cream, ampoule, etc. by mixing the prepared functional composition and other cosmetic ingredients. In addition, it may be formulated using a sterilization process of the obtained fermented extract of the plant with a sterile filter (0.45 to 0.2 μm).
실시예Example
2 L 삼각플라스크에 당나귀유 분말(Eurolactics Group SA, Lombardia, Italia)과 증류수로 20 g/L 농도로 조절하여 당나귀유 배지 1L를 제조하였다. 제조한 당나귀유 배지를 120℃에서 20분간 멸균하여 사용하였다.1 L of donkey milk medium was prepared by adjusting the concentration to 20 g/L with donkey milk powder (Eurolactics Group SA, Lombardia, Italia) and distilled water in a 2 L Erlenmeyer flask. The prepared donkey milk medium was sterilized at 120° C. for 20 minutes and used.
락토바실러스 사케이(Lactobacillus sakei)(KCCM40264), 락토바실러스 브레비스(Lactobacillus brevis)(KCCM35464), 락토바실러스 락티스(Lactobacillus lactis)(KCCM34717), 락토바실러스 카제이(Lactobacillus casei)(KCCM12452) 및 락토바실러스 아시도필루스(Lactobacillus acidophilus)(KCCM32820) 균은 한국미생물보존센터(KCCM) 및 American Type Culture Collection(ATCC)로부터 분양 받아 준비하였다. Lactobacillus sakei (KCCM40264), Lactobacillus brevis (KCCM35464), Lactobacillus lactis (KCCM34717), Lactobacillus casei (KCCM12452) and Lactobacillus asia Lactobacillus acidophilus (KCCM32820) was obtained from the Korea Center for Microbial Conservation (KCCM) and the American Type Culture Collection (ATCC).
상기 제조한 당나귀유 배지에 상기 5가지 균주를 각각 접종하고, 35℃에서 30시간 배양하여 각 프로바이오틱스의 당나귀유 배양액을 얻었다. 상기 얻어진 당나귀유 배양액을 6000rpm에서 10분 동안 원심분리한 후 상등액을 여과(0.45μm syringe filter, Millipore, Billerica, MA, USA)하여 프로바이오틱스 당나귀유 발효물을 얻었다. Each of the five strains was inoculated into the donkey milk medium prepared above, and cultured at 35° C. for 30 hours to obtain a donkey milk culture medium of each probiotic. The obtained donkey milk culture medium was centrifuged at 6000 rpm for 10 minutes, and the supernatant was filtered (0.45 μm syringe filter, Millipore, Billerica, MA, USA) to obtain a probiotic donkey milk fermented product.
얻어진 각각의 프로바이오틱스 당나귀유 발효물을 다음과 같은 배합비율로 혼합하여 화장료 조성물을 제조하였다.Each of the obtained probiotics and donkey milk fermented products were mixed in the following mixing ratio to prepare a cosmetic composition.
비교예comparative example
상기 실시예에서 제조한 당나귀유 배지에 락토바실러스 펜토서스(Lactobacillus pentosus)(KCCM43226)를 접종하고, 35℃에서 30시간 배양하여 당나귀유 배양액을 얻었다. 상기 얻어진 당나귀유 배양액을 6000rpm에서 10분 동안 원심분리한 후 상등액을 여과(0.45μm syringe filter,Millipore, Billerica, MA, USA)하여 당나귀유 발효물을 얻음으로써 화장료 조성물을 제조하였다. Lactobacillus pentosus (KCCM43226) was inoculated into the donkey milk medium prepared in the above example, and cultured at 35° C. for 30 hours to obtain a donkey milk culture medium. The obtained donkey milk culture medium was centrifuged at 6000 rpm for 10 minutes, and the supernatant was filtered (0.45 μm syringe filter, Millipore, Billerica, MA, USA) to obtain a fermented donkey milk product, thereby preparing a cosmetic composition.
실험예Experimental example
(1) 세포독성 시험(1) Cytotoxicity test
인간섬유아세포(Human Dermal fibroblast: HDFa)를 Medium 106에 Low Serum Growth Supplement를 혼합하여 페니실린(100IU/ml), 스트렙토마이신 (100μg/ml)를 함유하는 배지에서 37℃, 5% CO2를 포함하는 배양기내에서 배양하였다. 상기 제조된 화장료 조성물의 독성을 확인하기 위하여, 96 well plate에 세포를 분주한 다음 세포 배양조건에서 18시간 배양하였다. 배지를 제거하고 PBS로 세척한 후, 새로운 배지와 화장료 조성물을 각각 0.1, 0.5, 1, 2, 5% 농도로 넣고, 18시간 동안 배양하였다. 18시간 이후, 세포의 생존율을 측정하기 위해 살아있는 세포의 mitochondria 활성을 측정하여 세포의 생존율을 확인할 수 있는 MTT ASSAY를 수행하여 세포 생존율을 확인하였으며, 그 결과를 하기 표 2에 나타내었다. Human dermal fibroblast (HDFa) was mixed with Low Serum Growth Supplement in Medium 106 and cultured in a medium containing penicillin (100IU/ml) and streptomycin (100μg/ml) at 37℃ and 5% CO 2 Cultured in an incubator. In order to confirm the toxicity of the cosmetic composition prepared above, cells were dispensed into a 96 well plate and then cultured for 18 hours under cell culture conditions. After removing the medium and washing with PBS, fresh medium and the cosmetic composition were added at concentrations of 0.1, 0.5, 1, 2, and 5%, respectively, and cultured for 18 hours. After 18 hours, cell viability was confirmed by performing MTT ASSAY, which can confirm cell viability by measuring mitochondria activity of living cells in order to measure cell viability, and the results are shown in Table 2 below.
표 2에 나타난 바와 같이, 섬유아세포에 대해 비교예에 따른 화장료 조성물과 비교하여 본원 발명의 화장료 조성물은 0.1 내지 5% 농도 범위까지 100% 이상의 높은 세포 생존율을 보였다. 특히 0.1 내지 0.5% 농도일 때 가장 우수한 세포 생존률을 보이는 것으로 알 수 있다. As shown in Table 2, compared to the cosmetic composition according to Comparative Example, the cosmetic composition of the present invention showed a high cell viability of 100% or more in the concentration range of 0.1 to 5% for fibroblasts. In particular, it can be seen that the best cell viability is exhibited when the concentration is 0.1 to 0.5%.
(2) 염증 완화 시험(2) Inflammation mitigation test
염증 반응이 있을 때 발현이 증가하는 산화질소 생성효소 iNOS (inducible nitric oxide synthase)에 대한 본 발명에 따른 화장료 조성물의 저해 활성을 시험하였다.The inhibitory activity of the cosmetic composition according to the present invention on inducible nitric oxide synthase (iNOS), an enzyme whose expression is increased when there is an inflammatory reaction, was tested.
3 x 105 cells/ml의 RAW 264.7 (ATCCㄾIB-71TM)세포를 10% FBS (Fetal bovine serum)가 포함된(Dulbeco's Modified Eagle's Medium (DMEM) 배지(medium))로 24-웰 플레이트의 각 웰에 1ml씩 넣고 24시간 배양하였다. 24시간 후 부착된 세포를 PBS (phosphate-buffered saline)으로 세척 후 10% FBS-DMEM 배지와 상기 화장료 조성물을 각각 2.5, 5, 10, 20μg/ml 농도로 처리하고 30분 후, 염증을 유발하는 물질인 내독소로 1μg/ml LPS (lipopolysaccharide, 지질다당류)를 넣어 주고 37℃, 5% CO2에서 20시간 배양하였다. 블랭크(blank)로서 LPS 및 본 발명에 따른 화장료 조성물 첨가 없이 배양된 대식세포주인 RAW 264.7 세포(ATCCㄾTIB-71TM)와 대조군으로서 LPS만 첨가되어 배양된 RAW 264.7 세포를 사용하였다.3 x 10 5 cells/ml of RAW 264.7 (ATCC ㄾIB-71TM) cells were plated with 10% FBS (Fetal bovine serum) (Dulbeco's Modified Eagle's Medium (DMEM) medium) in each of a 24-well plate. 1 ml each was added to the well and incubated for 24 hours. After 24 hours, the attached cells were washed with PBS (phosphate-buffered saline), treated with 10% FBS-DMEM medium and the cosmetic composition at concentrations of 2.5, 5, 10, and 20 μg/ml, respectively, and after 30 minutes, inducing inflammation 1 μg/ml LPS (lipopolysaccharide, lipopolysaccharide) was added as endotoxin, and incubated at 37°C, 5% CO 2 for 20 hours. As a blank, RAW 264.7 cells (ATCC ® TIB-71TM), a macrophage cell line cultured without addition of LPS and the cosmetic composition according to the present invention, and RAW 264.7 cells cultured with only LPS added as a control were used.
각 웰의 상층액을 100μl씩 취하여 술파닐아미드(Sulfanilamide) 용액 및 N-(1-나프틸)-에틸렌디아민 (N-(1-naphtyl)-ethylenediamine) 용액과 각각 90μl씩과 반응시킨 후 540nm에서 흡광도를 측정하여 배양액에 생성된 질산염(nitrate) 또는 아질산염(nitrite)의 양을 확인하여 그 결과를 하기 표 3에 나타내었다. 100 μl of the supernatant from each well was reacted with 90 μl each of a sulfanilamide solution and a N-(1-naphthyl)-ethylenediamine solution, and then at 540 nm. The absorbance was measured to confirm the amount of nitrate or nitrite produced in the culture medium, and the results are shown in Table 3 below.
NaNO2 용액을 이용하여 표준 검량선을 작성하고 측정한 흡광도를 바탕으로 대조군에 대한 실험군의 각 실험 물질 처리에 따른 NaNO2 농도로 환산하여 LPS만 처리한 군과 비교하는 방법으로 시료의 산화질소(NO) 생성 저해 활성을 평가하였다. 질산염 농도로 저해율(%)을 구한 후 IC50 값을 산출하였다. IC50 값은 50% 억제율에 대한 시험 물질의 농도이다.A standard calibration curve was drawn up using the NaNO 2 solution , and based on the measured absorbance, the nitric oxide (NO ) production inhibitory activity was evaluated. After obtaining the inhibition rate (%) from the nitrate concentration, the IC 50 value was calculated. The IC 50 value is the concentration of the test substance for 50% inhibition.
본 발명에 따른 화장료 조성물은 2.5, 5, 10, 20μg/ml의 농도 처리시 LPS 단독 처리구에 비해 NO생성이 각각 농도 의존적으로 16.9, 36.4, 46.7, 65.8% 억제되었고, IC50은 15.1μg/ml 이었다. 상기 결과는 본 발명에 따른 화장료 조성물이 세포에서 iNOS 활성 저해를 통해 NO 생성을 억제하여 염증 반응을 근본적이고 효과적으로 억제할 수 있음을 나타낸다.The cosmetic composition according to the present invention inhibited NO production by 16.9, 36.4, 46.7, and 65.8% in a concentration-dependent manner, respectively, compared to the LPS-only treatment when treated at concentrations of 2.5, 5, 10, and 20 μg/ml, and the IC 50 was 15.1 μg/ml. was The above results indicate that the cosmetic composition according to the present invention can fundamentally and effectively inhibit inflammatory responses by inhibiting NO production through inhibition of iNOS activity in cells.
(3) 미백 효능 시험(3) whitening efficacy test
본 발명에 따른 화장료 조성물의 멜라닌 생성 억제능을 확인하기 위하여, 멜라닌 유도 물질인α-MSH를 피부암세포에 함께 처리하여 배양한 뒤 화장료 조성물을 0.1, 0.5% 로 각각 처리하여 3일간 배양 후 세포를 모았다. 모아진 세포를 1N NaOH 1mL을 가한 후 가열하여 세포를 완전히 녹인 뒤 475nm에서 흡광도를 측정했다. 음성대조군으로는 화장품 조성물을 처리하지 않은 것으로 처리했을 때와 비교하고 멜라닌양을 확인했다. 양성 대조군으로는 미백활성 보유 물질로 잘 알려진 알부틴 (Arbutin)을 0.005%로 사용하였다. 그 실험결과를 표 4에 나타내었다.In order to confirm the melanin production inhibitory ability of the cosmetic composition according to the present invention, skin cancer cells were treated with α-MSH, a melanin-inducing substance, and cultured, and then the cosmetic composition was treated with 0.1 and 0.5%, respectively, and the cells were collected after culturing for 3 days. . After adding 1 mL of 1N NaOH to the collected cells, the cells were completely dissolved by heating, and the absorbance was measured at 475 nm. As a negative control group, the amount of melanin was confirmed by comparing with the case where the cosmetic composition was not treated. As a positive control, 0.005% of Arbutin, which is well known as a substance having whitening activity, was used. The experimental results are shown in Table 4.
표 4에 나타난 바와 같이 본 발명에 따른 화장료 조성물을 0.1, 0.5% 로 각각 처리하였을 시 멜라닌 생성양이 처리 물질의 농도와 비례하여 감소하여 우수한 미백활성을 갖는 것을 알 수 있다.As shown in Table 4, when the cosmetic composition according to the present invention was treated at 0.1% and 0.5%, respectively, the amount of melanin produced decreased in proportion to the concentration of the treated material, indicating that it had excellent whitening activity.
(4) 티록시나제 억제 효과(4) Thyroxinase inhibitory effect
마우스 피부암세포(B16F10)를 페니실린(100IU/ml), 스트렙토마이신(100μg/ml), 10% FBS를 함유하는 DMEM(Dulbeco's Modified Eagle's Medium) 배지에서 37℃ 5% CO2를 포함하는 배양기내에서 배양하였다.Mouse skin cancer cells (B16F10) were cultured in DMEM (Dulbeco's Modified Eagle's Medium) medium containing penicillin (100IU/ml), streptomycin (100μg/ml), and 10% FBS in an incubator containing 5% CO 2 at 37°C. did
화장료 조성물의 티로시나아제 억제능을 확인하기 위하여, 상기 제조된 화장료 조성물과 멜라닌 유도 물질인 α-MSH를 세포에 함께 처리하여 3일간 배양 후 RNA를 추출하여 RT-PCR을 시행하였다. 증폭된 유전자는 Gel documentation system과 Image J 프로그램을 이용하여 분석하였다. 양성 대조군으로는 미백활성 보유 물질로 잘 알려진 알부틴(Arbutin)을 0.005%로 사용하였다. 그 실험결과를 표 5에 나타내었다.In order to confirm the tyrosinase inhibitory ability of the cosmetic composition, the prepared cosmetic composition and α-MSH, a melanin-inducing substance, were treated with the cells, cultured for 3 days, RNA was extracted and RT-PCR was performed. Amplified genes were analyzed using the Gel documentation system and Image J program. As a positive control, 0.005% of Arbutin, which is well known as a substance having whitening activity, was used. The experimental results are shown in Table 5.
표 5에 나타난 바와 같이 화장료 조성물을 0.1, 0.5% 로 각각 처리하였을 시 티로시나아제 발현이 농도와 비례하여 감소하여 본 발명의 화장료 조성물에 의한 멜라닌 억제가 멜라닌 생성반응의 핵심 단백질인 티로시나아제 효소 활성억제에서 기인함을 알 수 있다.As shown in Table 5, when the cosmetic composition was treated with 0.1 and 0.5%, respectively, the expression of tyrosinase decreased in proportion to the concentration, so that the melanin inhibition by the cosmetic composition of the present invention was the tyrosinase enzyme, a key protein in melanin production reaction. It can be seen that it is due to activity inhibition.
(5) 피부 보습 효과 시험(5) Skin moisturizing effect test
본원 발명의 화장료 조성물의 피부 보습 효과를 확인하기 위해 다음과 같은 시험을 추가적으로 실시하였다. 피부에는 결합수(Bound water)가 많을수록 보습 효과가 좋으며, 보습 효과가 장시간 유지될 수 있다.In order to confirm the skin moisturizing effect of the cosmetic composition of the present invention, the following tests were additionally conducted. The more bound water in the skin, the better the moisturizing effect, and the moisturizing effect can be maintained for a long time.
참고로, 일반적으로 물은 생채 내부에서 단백질, 탄수화물 등과 수소 결합을 통해 존재하는데 이러한 상태에 있는 물을 결합수라 부르며, 단백질, 탄수화물 등과 수소 결합한 상태에 있지 않은 자유수(free water)와 다른 거동 예를 들어, 0℃에서 얼지 않으며 100℃에서 증발되지 않는 거동을 보인다.For reference, in general, water exists inside living organisms through hydrogen bonds with proteins and carbohydrates. For example, it does not freeze at 0 ° C and does not evaporate at 100 ° C.
상기 제조된 화장료 조성물과 대조군(Glycerin, 2%)을 대상으로 보습력을 확인하였으며, 이는 Corneometer® CM825(Courage and Khazaka electronic GmbH, Germany)를 이용하여 측정하였다.Moisturizing power was confirmed for the cosmetic composition prepared above and the control group (Glycerin, 2%), which was measured using a Corneometer® CM825 (Courage and Khazaka electronic GmbH, Germany).
상기 화장료 조성물을 사용하기 전 피부 수분함량을 측정한 후 사용한 후 시간에 따른 피부 수분 함량을 재측정하였다. 또한, 상기 화장료 조성물을 사용한 후 120분이 경과할 때의 피부 수분함량과 사용 전 수분함량의 수분함량 증가율을 측정하였으며 측정 결과는 표 6에 나타내었다.After measuring the skin moisture content before using the cosmetic composition, the skin moisture content was remeasured according to time after use. In addition, the skin moisture content when 120 minutes elapsed after using the cosmetic composition and the moisture content increase rate of the moisture content before use were measured, and the measurement results are shown in Table 6.
((사용후-사용전)/사용전)Increase rate of skin moisture content
((After use-Before use)/Before use)
표 6에 나타난 바와 같이 본 발명에 따른 화장료 조성물을 0.1%, 0.5%로 각각 처리하였을 시 보습력이 처리 물질의 농도와 비례하여 증가하여 우수한 보습활성을 갖는 것을 알 수 있다.As shown in Table 6, it can be seen that when the cosmetic composition according to the present invention was treated with 0.1% and 0.5%, respectively, the moisturizing power increased in proportion to the concentration of the treated material, resulting in excellent moisturizing activity.
전술한 각 실시예에서 예시된 특징, 구조, 효과 등은 실시예들이 속하는 분야의 통상의 지식을 가지는 자에 의하여 다른 실시예들에 대해서도 조합 또는 변형되어 실시 가능하다. 따라서 이러한 조합과 변형에 관계된 내용들은 본 발명의 범위에 포함되는 것으로 해석되어야 할 것이다.The features, structures, effects, etc. illustrated in each of the above-described embodiments can be combined or modified with respect to other embodiments by those skilled in the art in the field to which the embodiments belong. Therefore, contents related to these combinations and variations should be construed as being included in the scope of the present invention.
Claims (11)
Complex probiotics comprising Lactobacillus sakei, Lactobacillus brevis, Lactobacillus lactis, Lactobacillus casei and Lactobacillus acidophilus A cosmetic composition comprising a fermented donkey's milk as an active ingredient.
상기 락토바실러스 사케이(Lactobacillus sakei)의 당나귀유 발효물은 상기 복합 프로바이오틱스의 당나귀유 발효물 전체 중량에 대하여 1 내지 15 중량%로 포함되는 화장료 조성물.
According to claim 1,
The cosmetic composition of claim 1, wherein the fermented donkey milk of Lactobacillus sakei is contained in an amount of 1 to 15% by weight based on the total weight of the fermented donkey milk of the complex probiotics.
상기 락토바실러스 브레비스(Lactobacillus brevis)의 당나귀유 발효물은 상기 복합 프로바이오틱스의 당나귀유 발효물 전체 중량에 대하여 5 내지 25 중량%로 포함되는 화장료 조성물.
According to claim 1,
The cosmetic composition comprising the fermented donkey milk of Lactobacillus brevis in an amount of 5 to 25% by weight based on the total weight of the fermented donkey milk of the complex probiotics.
상기 락토바실러스 락티스(Lactobacillus lactis)의 당나귀유 발효물은 상기 복합 프로바이오틱스의 당나귀유 발효물 전체 중량에 대하여 20 내지 40 중량%로 포함되는 화장료 조성물.
According to claim 1,
The cosmetic composition of claim 1, wherein the fermented donkey milk of Lactobacillus lactis is contained in an amount of 20 to 40% by weight based on the total weight of the fermented donkey milk of the complex probiotics.
상기 락토바실러스 카제이(Lactobacillus casei)의 당나귀유 발효물은 상기 복합 프로바이오틱스의 당나귀유 발효물 전체 중량에 대하여 15 내지 35 중량%로 포함되는 화장료 조성물.
According to claim 1,
The cosmetic composition of claim 1, wherein the fermented donkey milk of Lactobacillus casei is contained in an amount of 15 to 35% by weight based on the total weight of the fermented donkey milk of the complex probiotics.
상기 락토바실러스 아시도필루스(Lactobacillus acidophilus)의 당나귀유 발효물은 상기 복합 프로바이오틱스의 당나귀유 발효물 전체 중량에 대하여 5 내지 25 중량%로 포함되는 화장료 조성물.
According to claim 1,
The cosmetic composition comprising the fermented donkey milk of Lactobacillus acidophilus in an amount of 5 to 25% by weight based on the total weight of the fermented donkey milk of the complex probiotics.
A whitening and trouble care functional cosmetic comprising the cosmetic composition according to any one of claims 1 to 6.
락토바실러스 사케이(Lactobacillus sakei), 락토바실러스 브레비스(Lactobacillus brevis), 락토바실러스 락티스(Lactobacillus lactis), 락토바실러스 카제이(Lactobacillus casei) 및 락토바실러스 아시도필루스(Lactobacillus acidophilus)를 상기 당나귀유 배지에 각각 접종한 후 배양하는 배양단계(S20);를 포함하는 복합 프로바이오틱스의 당나귀유 발효물 제조방법.
A medium preparation step (S10) of preparing an aqueous solution of donkey milk powder to form a donkey milk medium; and
Lactobacillus sakei, Lactobacillus brevis, Lactobacillus lactis, Lactobacillus casei and Lactobacillus acidophilus were added to the donkey milk medium A method for producing a fermented donkey milk product of complex probiotics comprising: culturing step (S20) of inoculating and then culturing each.
상기 배지제조단계(S10)는 당나귀유 분말을 이용하여 10 내지 40 g/L 의 당나귀유 분말 수용액을 제조하고 멸균하여 당나귀유 배지를 제조하는 단계인 복합 프로바이오틱스의 당나귀유 발효물 제조방법.
According to claim 8,
The medium preparation step (S10) is a step of preparing a donkey milk medium by preparing a 10 to 40 g/L donkey milk powder aqueous solution using donkey milk powder and sterilizing it.
상기 배양단계(S20)는 상기 락토바실러스 사케이(Lactobacillus sakei), 락토바실러스 브레비스(Lactobacillus brevis), 락토바실러스 락티스(Lactobacillus lactis), 락토바실러스 카제이(Lactobacillus casei) 및 락토바실러스 아시도필루스(Lactobacillus acidophilus)를 상기 당나귀유 배지에 5 내지 10중량%로 각각 접종하고 30 내지 40℃의 혐기 조건에서 24 내지 36 시간 배양하는 단계인 복합 프로바이오틱스의 당나귀유 발효물 제조방법.
According to claim 8,
In the culturing step (S20), the Lactobacillus sakei, Lactobacillus brevis, Lactobacillus lactis, Lactobacillus casei and Lactobacillus acidophilus ( Lactobacillus acidophilus) in the donkey milk medium at 5 to 10% by weight, respectively, and culturing for 24 to 36 hours under anaerobic conditions at 30 to 40 ° C.
락토바실러스 사케이(Lactobacillus sakei), 락토바실러스 브레비스(Lactobacillus brevis), 락토바실러스 락티스(Lactobacillus lactis), 락토바실러스 카제이(Lactobacillus casei) 및 락토바실러스 아시도필루스(Lactobacillus acidophilus)를 각각 상기 당나귀유 배지에 접종한 후 배양하는 배양단계(S20);
상기 각각 배양된 당나귀유 배양액을 원심분리 후 여과하여 균주를 제거하고 당나귀유 발효물을 얻는 정제단계(S30); 및
상기 정제단계에 의해 얻어진 각각의 당나귀유 발효물을 혼합하는 혼합단계(S40);를 포함하는 화장료 조성물 제조방법.A medium preparation step (S10) of preparing an aqueous solution of donkey milk powder to form a donkey milk medium;
Lactobacillus sakei, Lactobacillus brevis, Lactobacillus lactis, Lactobacillus casei and Lactobacillus acidophilus, respectively, were added to the donkey milk A culture step of culturing after inoculation into a medium (S20);
A purification step (S30) of centrifuging and filtering each cultured donkey milk culture medium to remove strains and obtaining a fermented donkey milk culture; and
A cosmetic composition manufacturing method comprising a mixing step (S40) of mixing each fermented donkey milk obtained by the refining step.
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| KR101490710B1 (en) | 2013-04-04 | 2015-02-06 | 이대윤 | Cosmetics composition comprising donkey milk and method of fabricating thereof |
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| KR101849885B1 (en) | 2017-10-27 | 2018-04-18 | 주식회사 비앤비코리아 | Cosmetics comprising whey obtained by fermentation with Korean type lactic acid bacteria complex for improving elasticity or moisturizing |
| KR102015180B1 (en) * | 2017-10-30 | 2019-08-27 | 건국대학교 산학협력단 | Cosmetic composition comprising the fermented milk-cream broth of lactic acid bacteria |
| KR102026366B1 (en) * | 2018-05-11 | 2019-09-27 | 주식회사 네이처인랩 | Cosmetic composition comprising whey fermented product of complex probiotics, production methode thereof and functional cosmetic composition comprising the same |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR101490710B1 (en) | 2013-04-04 | 2015-02-06 | 이대윤 | Cosmetics composition comprising donkey milk and method of fabricating thereof |
| KR101846796B1 (en) | 2017-10-27 | 2018-04-06 | 주식회사 비앤비코리아 | Cosmetics comprising whey obtained by fermentation with Korean type lactic acid bacteria for improving elasticity or moisturizing |
| KR101849885B1 (en) | 2017-10-27 | 2018-04-18 | 주식회사 비앤비코리아 | Cosmetics comprising whey obtained by fermentation with Korean type lactic acid bacteria complex for improving elasticity or moisturizing |
| KR102015180B1 (en) * | 2017-10-30 | 2019-08-27 | 건국대학교 산학협력단 | Cosmetic composition comprising the fermented milk-cream broth of lactic acid bacteria |
| KR102026366B1 (en) * | 2018-05-11 | 2019-09-27 | 주식회사 네이처인랩 | Cosmetic composition comprising whey fermented product of complex probiotics, production methode thereof and functional cosmetic composition comprising the same |
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