KR101849885B1 - Cosmetics comprising whey obtained by fermentation with Korean type lactic acid bacteria complex for improving elasticity or moisturizing - Google Patents

Abstract

The present invention relates to cosmetics comprising whey obtained by fermentation with Lactobacillus pentosus GFC03 strain and a lactobacillus bombicola IB3 strain, which are Korean type complex lactic acid bacteria, wherein the cosmetics remarkably increase the elasticity of the skin and have excellent moisturizing effect to be usefully used as a functional cosmetic product.

Description

[0001] The present invention relates to a cosmetic composition for improving elasticity or moisturizing cosmetics containing a whey obtained by fermentation with a Korean complex type lactic acid bacteria,

The present invention relates to a skin elasticity improving or moisturizing cosmetic comprising Lactobacillus pentosus GFC03, a Korean lactic acid bacterium, and whey obtained by fermenting milk with Lactobacillus bovicola IB3 strain.

Probiotics is a combination of Pro and Biotics, Pro means 'for', and 'Biotics' means life. The World Health Organization (WHO) defines probiotics as a living organism that produces good health effects. The history of the word probiotics is not long, but it has been thousands of years since it came into human life. Probiotics are also used in traditional foods that utilize fermentation, prevent corruption and increase nutritional value, from cheese and yogurt to kimchi and miso in Korea. A representative of these is lactic acid bacteria.

In the cosmetic field there used to date since the lactic acid bacteria culture commercialized in 1955, mainly Streptococcus genus (Streptococcus sp.), Lactobacillus genus (Lactobacillus sp.), Lactobacillus nose Syracuse in (Lactococcus sp.), Current Kono Stock genus ( Leuconostoc sp.) And Bifidobacterium sp.), Fermenting directly into the culture medium, filtering and extracting the raw material, and filtering and extracting the fermented material by inoculating the active material. . Lactobacillus pentosus is a lactobacillus lactic acid bacterium, which is known to be useful and safe . It is antimicrobial, antioxidant, anti-inflammatory, and immunosuppressive. As a useful strain, it is advantageously utilized in various industries such as dairy products, health functional foods, and cosmetics.

On the other hand, milk (milk) is a remnant liquid after coagulation of milk, and it is also called whey or whey. Milk contains water, protein, fat, carbohydrates, minerals, vitamins, and enzymes. Fat is the most important ingredient and the exception is skim milk. The addition of acid or cough enzyme to skim milk results in clotting, which is called curd and is the main component of milk, casein. The aqueous solution except for the curd is referred to as whey and contains 20% of the total protein. Whey contains lactose, lacto-albumin, lactoglobulin, and minerals. Lactoglobulin contains immunoglobulins, especially in colostrum. Whey has usually been regarded as a by-product of cheese products, but gradually its nutritional importance and health-promoting functionality have been found to be excellent. Since a significant portion of the milk component flows into the whey during cheese production, the content of protein, lactose, minerals and vitamins in whey is very high. On the other hand, not only cheese but also by-products after condensation of yogurt fermented products are also referred to as whey in the same sense.

As a technique for applying whey to a cosmetic material, in Korea, Korean Patent Registration No. 10-0030790 entitled " Method for Producing a Skin Moisturizing Agent ", in which a lactic acid bacterium is inoculated into an incubator having an animal milk as a main component, , The whey was heated to about 30-50 ° C under reduced pressure and about 10-20% of the whey was evaporated to evaporate a portion of the whey until the acetaldehyde, acetone and diacetyl components in the whey were less than 0.5 ppm A method for producing a moisturizer for a skin, which comprises the steps of:

Korean Patent Registration No. 10-0027759 entitled " Cosmetics Containing Antioxidants " discloses that an antioxidant obtained by substantially removing a high molecular weight substance having a molecular weight of 6000 or more from 0.03-3.0 wt% of whey collected from a culture solution of lactic acid bacteria using an animal's ex- A cosmetic composition containing an antioxidant is disclosed.

Therefore, the inventors of the present invention found that whey-containing cosmetics prepared from Lactobacillus pentosus strain GFC03 isolated from kimchi, which is a Korean traditional food, and Lactobacillus bovicola IB3 strain isolated from Bacillus subtilis have skin elasticity effect and skin moisturizing effect The present invention has been completed.

Korean Patent No. 10-0030790 (the name of the invention: a method for producing a skin moisturizer, applicant: Yakult Honsha Co., Ltd., registered on December 13, 1989) Korean Patent No. 10-0027759 (Name of the invention: Cosmetics containing antioxidants, Applicant: Yakult Honsha Co., Ltd., Disclosure Date: Apr. 06, 1989)

It is an object of the present invention to provide a skin elasticity improving or moisturizing cosmetic comprising Lactobacillus pentosus GFC03, a Korean lactic acid bacterium, and whey obtained by fermenting milk with Lactobacillus bovicola IB3 strain.

The present invention relates to a method for producing a lactic acid bacterium containing a whey obtained by fermentation of milk with a strain of Lactobacillus pentosus GFC03 (Korean Lactobacillus pentosus GFC03, KCCM 4326), Lactobacillus bombicola IB3 ( Lactobacillus bombicola IB3, Accession No .: KCCM 43227) To a cosmetic composition for improving elasticity or moisturizing.

The whey may be a supernatant obtained by centrifuging the yogurt obtained by inoculating and culturing Lactobacillus pentosus GFC03 strain and Lactobacillus bovicola IB3 strain into milk.

The culturing is performed at 28 to 33 DEG C for 2 to 7 days.

The composition may include rosemary leaf extract, pomegranate extract, mangosteen bark extract, butterfly bean flower extract, donkey oil and milk protein extract.

The composition may further comprise shea butter, argane tincture oil, olive oil and sunflower seed oil.

Hereinafter, the present invention will be described in detail.

The present invention relates to a method for producing a lactic acid bacterium containing a whey obtained by fermentation of milk with a strain of Lactobacillus pentosus GFC03 (Korean Lactobacillus pentosus GFC03, KCCM 4326), Lactobacillus bombicola IB3 ( Lactobacillus bombicola IB3, Accession No .: KCCM 43227) The present invention relates to a cosmetic composition for improving or improving elasticity, wherein the whey contains 0.0001 to 10% by weight, preferably 0.001 to 10% by weight, of the cosmetic composition in a cosmetic formulation prepared from a cosmetic composition as a raw material.

Lactobacillus pentosus GFC03 is isolated from kimchi, Lactobacillus bovicola IB3 is isolated from the subfamily field, and can be inoculated into a lactic acid bacteria culture medium and then maintained at 28 to 33 ° C for 2 to 7 days under anaerobic conditions. For the production of whey, it is preferred that the Lactobacillus pentosus GFC03 strain and Lactobacillus bovis cola IB3 are cultured in the same manner using milk instead of the MRS medium to obtain yogurt, and the supernatant is obtained after centrifuging the yogurt.

The initial inoculation concentration to the MRS medium or milk for the cultivation of the Lactobacillus pentosus GFC03 strain and the Lactobacillus pentosus GFC03 strain is preferably 1 × 10 ^{6 to 8} cfu / ml. In order to obtain a whey having better fermentation efficiency, it is preferable to ferment milk by inoculation after mixing Lactobacillus pentosus GFC03 strain and Lactobacillus pentosus GFC03 (a culture solution containing cells or cells). At this time, it is more preferable that the mixing range of the strains is 1: 0.5 to 2 by volume or weight ratio, but it is most preferable to ferment milk by inoculation after mixing the same cell mass.

The lactic acid bacteria culture medium is preferably an MRS liquid culture medium, and examples thereof include MRS medium (Proteose peptone) No. 1. 3. 10 g of Beef extract, 5.0 g of Yeast extract, 20 g of Dextrose, 1.0 g of Polysorbate 80, 2.0 g of Ammonium citrate, Water was added so that the total amount of the solution was 1 L, 5.0 g of sodium acetate, 0.1 g of magnesium sulfate, 0.05 g of manganese sulfate, 2.0 g of dipotassium phosphate and 15.0 g of agar It is preferable to use it by sterilization.

The extract contained in the composition of the present invention may be obtained by extracting each of the raw materials with water, a C1 to C4 alcohol or an aqueous solution thereof, butylene glycol or an aqueous solution thereof and a solvent of a group consisting of hexane. Preferably a water extract.

The cosmetic composition containing the whey of the present invention may include rosemary leaf extract, pomegranate extract, mangosteen bark extract, butterfly bean flower extract, donkey oil and milk protein extract. The composition may contain rosemary leaves 1 to 10 parts by weight of an extract, 10 to 100 parts by weight of a pomegranate extract, 5 to 50 parts by weight of a mangosteen extract, 5 to 50 parts by weight of a butterfly flower extract, 100 to 500 parts by weight of a donkey oil, Weight portion may be included.

The pomegranate extract may be any of leaves, fruits, and stem extracts of pomegranate, but is preferably a fruit extract.

In addition, the cosmetic composition containing the whey may further comprise shea butter, argane tincture oil, olive oil and sunflower seed oil. When the composition is 100% by weight, 3 to 10% by weight of shea butter, 0.1 to 1 wt% of kernel oil, 0.1 to 1 wt% of olive oil, and 0.1 to 4 wt% of sunflower seed oil.

In this case, even if only the whey is included, the composition of the present invention is excellent in the elasticity improvement or moisturizing effect, but the rosemary leaf extract, pomegranate extract, mangosteen bark extract, butterfly bean flower extract, donkey oil, , The inhibitory effect or moisturizing effect of the elastase is further enhanced, which can be further enhanced if shea butter, argane tincture oil, olive oil and sunflower seed oil are included in the above weight range.

The donkey oil used in the present invention is powdered donkey milk. The milk protein extract is a milk glycoprotein commonly used in cosmetic raw materials. Preferably, the milk protein is hydrolyzed with casein K obtained from milk to lyin, It is spray-dried powder after purification. It contains 3.0-6.0% of nitrogen (N: 14.01) when quantified.

The arganic tincture oil, olive oil or sunflower seed oil used in the present invention is obtained by squeezing the seeds of each plant. It is possible to carry out extraction at a low temperature or extraction at a high temperature, but it is preferable to extract at low temperature to protect the skin.

The shear butter used in the present invention is obtained by extracting only the fatty acid which is formed in a liquid state by melting the fruit seed of the whole tree into hexane-based chemicals and is mainly used as a cosmetic raw material.

In the production of the extract used in the present invention, the extraction solvent may be 1 to 40 times by volume (1 to 40 liters per 1 kg), preferably 5 to 40 times by volume. In this case, the extraction conditions may be 1 minute to 48 hours at 20 to 100 ° C. The above process can be repeated 1 to 4 times. As the extraction apparatus, a conventional extraction apparatus, an ultrasonic pulverization extractor, or a fractionator may be used. The thus prepared extract can be removed by hot air drying, vacuum drying or freeze drying. The extract may be purified by enzymatic treatment or column chromatography. The above-mentioned extract can be isolated or isolated by known methods used for separation and extraction of plant components, such as extraction with an organic solvent (alcohol, ether, acetone, etc.), hexane and water, And may be fractionated or purified using a combination method. The chromatography can be carried out using silica gel column chromatography, LH-20 column chromatography, ion exchange resin chromatography, medium pressure liquid chromatography chromatography, thin layer chromatography (TLC), silica gel vacuum liquid chromatography, and high performance liquid chromatography.

The formulations of the cosmetics are not particularly limited, but preferably include cosmetics such as external ointments, creams, softening supplements, nutritional lotions, packs (washoffs, peel-offs, sheet mask liquids), essences, hair tonics, shampoos, Lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutritional cream, eye cream, moisturizing cream, hand cream, foundation, nutrition essence, sunscreen, skin lotion, skin toner, , Soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions and body cleansers.

The cosmetics of the present invention may further contain ingredients selected from the group consisting of water-soluble vitamins, oiliness vitamins, polymer peptides, polymeric polysaccharides, sphingolipids and seaweed extracts.

The water-soluble vitamin is not particularly limited as long as it can be compounded in cosmetics. Preferably, vitamin B, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, And their salts (thiamine hydrochloride, sodium ascorbate, etc.) or derivatives (sodium ascorbic acid-2-phosphate, magnesium ascorbate-2-phosphate etc.) can also be added to water-soluble vitamins . The water-soluble vitamin can be obtained by a conventional method such as a microorganism conversion method, a purification method from a culture of a microorganism, an enzymatic method, or a chemical synthesis method.

Usable vitamins include vitamins such as vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol) Alpha-tocopherol vitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantothenyl ethyl laurate, and the derivatives thereof (palmitic acid ascorbin, stearic acid ascorbic acid, dipalmitic acid ascorbin, dl-alpha tocopherol acetate, Ether, etc.) are also included in the usable vitamins used in the present invention. Usability Vitamins can be obtained by a conventional method such as a microorganism conversion method, a purification method from a culture of a microorganism, an enzyme or a chemical synthesis method.

The polymeric peptide may be any compound as long as it can be compounded in cosmetics, and examples thereof include collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, and keratin. The polymeric peptide can be obtained by a conventional method such as purification from a culture broth of a microorganism, an enzymatic method, or a chemical synthesis method, or it can be purified from natural products such as ducks such as pigs and cows and silk fiber of silkworms.

The polymeric polysaccharide may be any compound as long as it can be incorporated in cosmetics, and examples thereof include hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.). For example, chondroitin sulfate or a salt thereof can be usually purified from mammals or fish.

Sphingo lipids may be any as long as they can be incorporated into cosmetics, and preferable examples thereof include ceramides, phytosphingosine and sphingoglycolipids. Sphingoid lipids can be purified from ordinary mammals, fish, shellfish, yeast or plants by conventional methods or can be obtained by chemical synthesis.

The seaweed extract may be any of those which can be compounded in cosmetics. Preferably, the seaweed extract is selected from the group consisting of algae extract, red pepper extract, green algae extract and the like. Also, the algae extract may be colored guanine, arginic acid, Potassium alginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained from seaweed by a conventional method.

The cosmetic of the present invention may also be blended with other ingredients to be blended with ordinary cosmetics.

Examples of the compounding ingredients that may be added include organic solvents such as a preservative component, a moisturizer, an emollient, a surfactant, an organic and inorganic pigment, an organic powder, an ultraviolet absorbent, a preservative, a bactericide, an antioxidant, a plant extract, a pH adjuster, A blood circulation accelerator, a cold agent, an antiperspirant agent, and purified water.

Examples of the oil retaining component include ester-based oil retaining, hydrocarbon-based oil retaining, silicone-based oil retaining, fluoric oil retaining, animal retention and plant retention.

Examples of ester-based fats include glyceryl tri-2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isostearyl isostearate, Butyl isopropyl myristate, isopropyl myristate, isopropyl myristate, isopropyl myristate, isopropyl myristate, isopropyl myristate, butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isosilyl myristate, isostearic acid isostearyl, isostearyl palmitate, octyldodecyl myristate, Trimethylol propane, triisostearic acid trimethylol propane, tetra 2-ethylhexanoic acid pentaerythritol tetra (2-ethylhexanoate) , Decyl caprylate, decyl laurate, hexyl laurate, myristate decyl, myristyl myristate, myristine monoethyl stearate, stearyl stearate, decyl oleate, ricinoleic acid tri , Isostearyl stearate, isostearyl stearate, isodecyl stearate, octyldodecyl oleate, octyldodecyl linoleate, isopropyl isostearate, isopropyl stearate, isopropyl stearate, isopropyl stearate, -Hexyl stearate, stearyl ethylhexanoate, stearyl 2-ethylhexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicaprate, di (capryl, capric acid) propylene glycol, Propyleneglycol propionate, propyleneglycol propionate, dicaproic acid neopentyl glycol, dioctanoic acid neopentyl glycol, tricarboxylic acid glyceryl, triunsaturated glyceryl, triisopalmitic acid glyceryl, triisostearic acid glyceryl, neopentanoic acid octyldodecyl Octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, Octyldecyl lactate, octyldecyl lactate, octyldecyl lactate, polyglycerin oleic acid ester, polyglycerin isostearic acid ester, triisocetyl citrate, triisobutyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, But are not limited to, ethyl, acetyltriethyl citrate, acetyltributyl citrate, trioctyl citrate, diisostearyl malate, 2-ethylhexyl hydroxystearate, di-2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, But are not limited to, dioctyl sebacate, stearic acid cholesteryl, isostearic acid cholesteryl, hydroxystearic acid cholesteryl, oleic acid cholesteryl, oleic acid dihydrocholesteryl, isostearic acid pitostearyl, Stearoyl hydroxystearic acid isostearyl, 12-stearoyl stearyl hydroxystearate, 12-stearo And monohydroxystearic acid and esters such as sostearyl.

Examples of the hydrocarbon hydrocarbon-based fats include hydrocarbon fats and oils such as squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, floating isoparaffin, polybutene, microcrystalline wax and vaseline.

Examples of silicone based oils include polymethyl silicone, methylphenyl silicone, methyl cyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane-methylcetyloxysiloxane copolymer, dimethylsiloxane-methylstarchoxysiloxane copolymer, alkyl Modified silicone oils, and amino-modified silicone oils.

Examples of the fluorine-based oil include perfluoropolyether and the like.

Examples of the animal or vegetable oil include avocado oil, armond oil, sesame oil, rice bran oil, new flower oil, soybean oil, corn oil, rape oil, apricot kernel oil, palm kernel oil, palm oil, castor oil, Corn starch, lanolin wax, lanolin wax, lanolin wax, corn starch wax, liquid lanolin, corn starch wax, corn starch wax, liquid lanolin , Hardened castor oil, and the like.

Examples of the moisturizing agent include water-soluble low-molecular moisturizing agents, oil-soluble molecular moisturizing agents, water-soluble polymers, and oil-soluble polymers.

Examples of the water-soluble low-molecular moisturizing agent include serine, glutamine, sorbitol, mannitol, sodium pyrrolidone-carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B Glycol (polymerization degree n = 2 or more), polyglycerin B (polymerization degree n = 2 or more), lactic acid, lactic acid salt and the like.

Examples of the lipid-soluble low-molecular moisturizing agent include cholesterol and cholesterol ester.

Examples of the water-soluble polymer include carboxyvinyl polymer, polyaspartic acid, tragacanth, xanthan gum, methylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, carboxymethylcellulose, water-soluble chitin, chitosan, dextrin, etc. .

Examples of the oil-soluble polymer include polyvinylpyrrolidone / eicosene copolymer, polyvinylpyrrolidone / hexadecene copolymer, nitrocellulose, dextrin fatty acid ester, and polymer silicone.

Examples of the emollients include long chain acyl glutamic acid cholesteryl ester, hydroxystearic acid cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid and lanolin fatty acid cholesteryl ester.

Examples of the surfactant include nonionic surfactants, anionic surfactants, cationic surfactants, and amphoteric surfactants.

Examples of the nonionic surfactant include self emulsifying monostearate glycerin, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbit fatty acid ester, POE (Polyoxyethylene / polyoxypropylene) copolymer, POE.POP alkyl ether, polyether-modified silicone, polyether-modified silicone, polyoxyethylene-polyoxypropylene (POE) Alkanolamides, alkylamine oxides, hydrogenated soybean phospholipids, and the like.

Examples of the anionic surfactant include fatty acid soap, alpha-acylsulfonate, alkylsulfonate, alkylarylsulfonate, alkylnaphthalenesulfonate, alkylsulfate, POE alkyl ether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl ginseng salt, Alkylsulfosuccinic acid salts, acylated hydrolyzed collagen peptide salts, and perfluoroalkyl phosphoric acid esters, and the like can be mentioned. have.

Examples of the cationic surfactant include alkyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium bromide, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyl dimethyl benzyl ammonium chloride, behenyl trimethyl ammonium chloride, Benzalkonium, diethylaminoethylamide stearate, dimethylaminopropylamide stearate, quaternary ammonium salts of lanolin derivatives, and the like.

Examples of the amphoteric surfactant include carboxybetaine type, amide betaine type, sulfobetaine type, hydroxysulfobetaine type, amidosulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type and amide amine type Amphoteric surfactants and the like.

Examples of the organic and inorganic pigments include inorganic pigments such as silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, Bengala, clay, bentonite, titanium mica, titanium oxide, bismuth chloride, zirconium oxide, magnesium oxide, Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, chromium oxide, chromium oxide, chromium hydroxide, But are not limited to, polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluororesin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, Silk powder, cellulose, CI Pigment Yellow, CI Pigment Orange, and composite pigments of inorganic pigments and organic pigments thereof.

As the organic powder, metallic soap such as calcium stearate; Metal salts of alkyl phosphates such as sodium zinc cetylate, zinc laurylate and calcium lauryl laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc and N-lauroylglycine calcium; Amidosulfonic acid multivalent metal salts such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; Such as N-epsilon-lauroyl-L-lysine, N-epsilon-palmitoylidene, N-alpha-paratyylnitine, N-alpha-lauroyl arginine, Acyl basic amino acids; N-acylpolypeptides such as N-lauroylglycylglycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid, alpha-aminoaurauric acid, and the like; Polyethylene, polypropylene, nylon, polymethylmethacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride, and the like.

Examples of ultraviolet absorbers include paraaminobenzoic acid, ethyl parnamobenzoate, amyl paranobenzoate, octyl paranobenzoate, ethyleneglycol salicylate, phenyl salicylate, benzyl salicylate, benzyl salicylate, butylphenyl salicylate, homomenthyl salicylate, benzyl cinnamate , Octyl methoxycinnamate, dioctyl methoxycinnamate, mono-2-ethylhexane glyceryl dipyrromethoxycinnamate, isopropyl paratumoxycinnamate, diisopropyl-diisopropyl cinnamate ester mixture, Carninoic acid, ethyl urocanoate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenone sulfonic acid and salts thereof, dihydroxymethoxybenzophenone, sodium dihydroxymethoxybenzophenone disulfonate, dihydroxybenzophenone , Tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianylino-p- (carbo-2'-ethylhexyl- , 3,5-triazine, 2- (2-hydroxy Methyl-5-methylphenyl) benzotriazole, and the like.

Examples of the disinfectant include hinokitiol, trichloroacid, trichlorohydroxydiphenyl ether, crohexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, zinc filitione, benzalkonium chloride, No. 301, mononitro and eicol sodium, and undecylenic acid.

Examples of the antioxidant include butylhydroxyanisole, gallic acid propyl, and eicosorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fumaric acid, sodium fumarate, succinic acid, sodium succinate, sodium hydroxide, sodium monohydrogenphosphate and the like.

Examples of the alcohol include higher alcohols such as cetyl alcohol.

In addition, any of the above components may be blended within the range not to impair the objects and effects of the present invention, but is preferably 0.01 to 5% by weight, more preferably 0.01 to 5% by weight, May be formulated in an amount of 0.01 to 3% by weight.

The cosmetic of the present invention may take the form of a solution, an emulsion, a viscous mixture or the like.

The ingredients contained in the cosmetic composition of the present invention may contain, as an active ingredient, the ingredients commonly used in cosmetics in addition to the above-mentioned extracts. For example, conventional auxiliary substances such as stabilizers, solubilizers, vitamins, .

When the cosmetic formulation of the present invention is a paste, cream or gel, animal fibers, plant fibers, wax, paraffin, starch, tracant, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide .

When the cosmetic formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in the case of a spray, Propane / butane or dimethyl ether.

When the cosmetic formulation of the present invention is a solution or an emulsion, a solvent, a solvent or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethylcarbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid esters of sorbitan.

When the cosmetic formulations of the present invention are in the form of a suspension, the carrier component may be a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the cosmetic formulation of the present invention is an interface-active agent-containing cleansing, it is preferable to use, as a carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, Amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linolenic derivative or ethoxylated glycerol fatty acid ester.

The present invention relates to a cosmetic comprising Lactobacillus pentosus GFC03, a Korean lactic acid bacterium, and whey obtained by fermenting with a lactobacillus bovicola IB3 strain. The cosmetic composition remarkably increases skin elasticity and has a high moisturizing effect, Can be usefully used.

Fig. 1 shows the improvement rates of skin elasticity in the 2 nd and 4 th week of the subjects using the cosmetic composition of the present invention.
Fig. 2 shows the percent improvement in skin densitometric density at 2 and 4 weeks of the subjects using the cosmetic composition of the present invention.
Fig. 3 shows the skin moisture content of the subjects using the cosmetic composition of the present invention at the 2nd and 4th week.

Hereinafter, preferred embodiments of the present invention will be described in detail. However, the present invention is not limited to the embodiments described herein but may be embodied in other forms. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the concept of the invention to those skilled in the art.

≪ Example 1: Selection of fermenting bacteria &

First, the Kimchi samples were diluted to 10 ^-6 with 10 decane dilution method and then 10 g of MRS medium (Proteose peptone No. 3, 10 g of Beef extract, 5.0 g of yeast extract) containing 0.002% of bromophenol blue, 20 g of Dextrose, 1.0 g of Polysorbate 80, 2.0 g of ammonium citrate, 5.0 g of sodium acetate, 0.1 g of magnesium sulfate, 0.1 g of manganese sulfate (Colomony), which forms a yellow circle around the stomach, was applied to a candidate group of lactic acid bacteria (GFC01, ~ 04). Elastase and extracellular melanin production inhibition test were selected among the isolates, and the most effective GFC03 strain was selected. In addition, IB3, which was used as a comparative group or complex fermentation strain, was isolated from the subfamily field.

≪ Example 2: Determination of the genetic properties of strains >

In order to analyze the DNA sequence of 16s rRNA of the selected GFC03 or IB3 strain, gDNA of the finally selected GFC03 strain and the universal primer 27F (5 'AGA GTT TGA TCM TGG CTC AG 3') and 1492R (5 'TAC GGY TAC CTT GTT ACG ACTT 3 '), followed by DNA sequence analysis of 16S rRNA to confirm that it has the nucleotide sequence of SEQ ID NO: 1. Based on the DNA sequencing results of 16s rRNA, homology analysis was performed through BLAST search provided by NCBI.

Homology analysis GFC03 strains was confirmed with Lactobacillus pento suspension (Lactobacillus pentosus) and 98.76% similarity to belong to the Lactobacillus bacteria (Lactobacillus) genus (genus). Therefore, it was named Lactobacillus pentosus GFC03, deposited on August 19, 2016, and deposited under accession number KCCM 43226, to the Korean Microorganism Conservation Center (KCCM), an international microbiological patent depositary.

SEQ ID NO: 1:

Meanwhile, in the present invention, IB3 strain used as a subject to be compared or compound fermentation was confirmed to be a strain having DNA of 16s rRNA having the sequence of SEQ ID NO: 2 through the above strain identification method, and Lactobacillus IB3 bombicola IB3, Accession No .: KCCM 43227).

SEQ ID NO: 2:

≪ Example 3: Preparation of whey &

Lactobacillus pentosus GFC03 or Lactobacillus bulbicola IB3 strain was inoculated at a concentration of 1 × 10 ⁷ cfu / ml in 100 g of milk or 5 × 10 ⁶ cfu / ml of Lactobacillus pentosus GFC03 and Lactobacillus bulb cola IB3 1x10 < ⁷ > cfu / ml), and cultured for 3 days at 28 DEG C to prepare yogurt. The obtained yogurt was centrifuged and separated into a curd and a liquid phase (whey fermented product) as a supernatant, and whey except curd was obtained.

<Example 4: Wrinkle / elasticity test: Elastase activity inhibition>

The whey of Example 3 was diluted with various concentrations and then diluted with 1.6 mM N-succinyl-alanine-alanine-alanine-p-nitroanilide (N-succinyl- (Ala) 3- p-nitroanilide (S4760, Sigma) was added, and the same amount of the enzyme solution (Porcine Pancreatic Elastase standard: Porcine Pancreatic Elastase, SIGMA, E-1250) was added to the mixture and reacted at 25 ° C for 15 minutes. The amount of produced p-nitroaniline was measured at 410 nm to determine the anti-wrinkle / elasticity improving activity.

Elastase activity inhibition (%) = (absorbance of sample treated group / absorbance of untreated group) x 100

Elastase inhibition by whey concentration Treatment concentration Untreated group 1 wt% 2 wt% 4 wt% Lactobacillus pentosus GFC03 cultured whey 0% inhibition 11.02% inhibition 26.15% inhibition 83.98% inhibition Lactobacillus bovis cola IB3 cultured whey 0% inhibition 9.18% inhibition 15.79% inhibition 18% inhibition Lactobacillus pentosus GFC03 and Lactobacillus bovisca IB3
Complex culture whey 0% inhibition 51% inhibition 75% inhibition 92% inhibition

As a result, as shown in Table 1, it can be seen that the inhibitory effect of the Lactobacillus pentosus GFC03-whey cultured whey cultured in the same Lactobacillus cultured whey cultures on the inhibitory effect of the elastase was greater than that of the Lactobacillus bovicola IB3-cultured whey cultivars . In addition, uniquely, the inhibitory effect of Lactobacillus bovis cola IB3 itself on elastase inhibition is very low. However, whey, which is a combination of Lactobacillus pentosus GFC03 and lactobacillus pentosus GFC03, has a higher inhibitory effect on elastase than Lactobacillus pentosus GFC03 cultured whey You can see the excellence. Therefore, the cosmetic composition for improving the wrinkle or elasticity of the present invention uses this complex culture whey.

On the other hand, 1100 parts by weight of the milk protein extract, 6 parts by weight of the rosemary leaf extract, 50 parts by weight of the pomegranate extract, 19 parts by weight of the mangosteen bark extract, 19 parts by weight of the butterfly bean flower extract, And 250 parts by weight of a mixture (an extract-added group of the following Table 2) was tested at the same concentration instead of Lactobacillus bovicola IB3 cultured whey, and it was confirmed that an inhibitory effect of 50% or more was exhibited.

 Elastase inhibition rate by addition of extract Treatment concentration 0.5 wt% Lactobacillus pentosus GFC03 and Lactobacillus bovisca IB3
Complex culture whey 27% inhibition Lactobacillus pentosus GFC03 and Lactobacillus bovisca IB3
Complex cultured whey and extract added group 51% inhibition

< Example  5. For improving elasticity or for moisturizing Tension-up  Preparation of moisture cream>

1100 parts by weight of a milk protein extract, 6 parts by weight of a rosemary leaf extract, 50 parts by weight of a pomegranate extract, 50 parts by weight of a mangosteen bark extract, 100 parts by weight of a lactobacillus pentosis GFC03 and a lactobacillus bovisola IB3 complex culture whey, 19 parts by weight of a butterfly bean flower extract, 19 parts by weight of a butterfly bean flower extract and 250 parts by weight of a donkey oil was a main raw material sample.

At this time, the mixture was mixed with 0.5% by weight in total of the cream formulation, and each component in the cream formulation was prepared as shown in Table 3 below, and was prepared by a conventional cream formulation method.

ingredient Content (% by weight) Raw sample (mixed cultured whey to donkey oil mixed state) 0.500000 Sheer butter 6.000000 Sunflower seed oil 0.500000 Argan tree kernel oil 0.300000 Olive oil 0.300000 Butylene glycol 5.191000 Cetearyl alcohol 4.000000 glycerin 3.063485 Stearic acid 3.000000 Hydrogenated polydecene 3.000000 Cetyl hexanoate 3.000000 Dipropylene glycol 2.000000 Caprylic / capric triglyceride 2.000000 Beads wax 2.000000 Dimethicone 1.100000 Sorbitan sesquioleate 0.600000 Glyceryl stearate 0.520000 Polysorbate 60 0.500000 FAGE -100 stearate 0.480000 Cyclopentasiloxane 0.300000 Arginine 0.200000 Acrylate / C10-30 alkyl acrylate crosspolymer 0.200000 Purified water Balance Total 100.00000

&Lt; Example 6: Confirmation of elasticity improvement effect &

After the cream of Example 5 was applied to the eyes and face daily for 4 weeks, the effect of improving the elasticity was confirmed. Elasticity was measured using a cutometer (MPA 580, Courage and Khazaka Electronic Co., Germany) at a distance of 3 cm from the eye margin. The evaluation was carried out three times and the average of three values was used. The R2 value was selected as a variable, and the higher the value, the better the skin elasticity. In this test, the value of variable R2 was measured before use (0 weeks), 2 weeks after using the product, and 4 weeks after using the product. The measurement coefficient is arbitrary unit (A.U.). Also, the obtained results were confirmed by calculating the elasticity improvement rate (%).

Test results As shown in Fig. 1, the skin elasticity is remarkably increased during the application of the cream of Example 5 for 4 weeks.

Example 7. Confirmation of skin dermal density < RTI ID = 0.0 >

Skin moisture measurements of the subjects of Example 6 were measured with DERMA SCAN C Ver 3. COMPACT (CORTEX TECHNOLOGY, Denmark). This measurement process is confirmed by injecting distilled water into the water chamber equipped with the Scannig head and scanning it closely to the measurement site and imaging it. Distilled water serves as a medium for ultrasound. The Derma scan C instrumentation was compared with the intensity of the B-scan measurements using the DERMA SCAN C Ver 3. Program. The higher the value, the more dense the dermis. The dermal density analysis value is a value calculated by the program and expressed as a percentage unit (%). The results obtained were also calculated by calculating the percent improvement in dermal density (%).

As a result, as shown in FIG. 2, it can be seen that the skin dermis compactness improvement rate remarkably increased during application of the cream of Example 5 for 4 weeks.

&Lt; Example 8. Confirmation of moisturizing effect >

The skin moisture of the subjects of Example 6 was measured using a corneometer (CM825, Courage and Khazaka Electronic Co., Germany). Before the product was used (0 weeks), after 2 weeks of using the product, and after 4 weeks of using the product, the average value was obtained by using the three values measured by the corneometer. The higher the water content, the higher the measured value, and the measurement factor is the Arbitrary unit (A.U.). The results obtained were also calculated by calculating skin moisture improvement rate (%).

As a result, as shown in FIG. 3, skin moisture was significantly increased during application of the cream of Example 5 for 4 weeks.

&Lt; Example 9: Measurement of transdermal water loss amount >

Trans-Epidemal Water Loss (TEWL) was measured with a Tewameterr (TM300, Courage and Khazaka Electronic Co., Germany) using a cream formulation containing 0.5 wt% of each of the following raw materials 1 to 4. At this time, the control group was made of cream added with water instead of the raw material sample.

Raw sample 1: Lactobacillus bovicola IB3 strain fermented whey as disclosed in Example 3.

Raw material sample 2: Lactobacillus pentosus GFC03 fermented whey as disclosed in Example 3.

Raw material sample 3: Lactobacillus pentosus GFC03 and Lactobacillus bovicola IB3 complex cultured whey disclosed in Example 3.

Raw sample 4: A mixture of milk protein extract and donkey oil mixed in the complex culture whey described in Example 4. [

The cream formulation was prepared in the same manner except that each raw material sample in the cream of Example 5 was used.

Twenty women in their 20s and 40s in each group were divided uniformly into each cream, and the transdermal water loss was measured three times with Tewameterr (TM300, Courage and Khazaka Electronic Co., Germany) within 30 minutes Respectively. The average value was used to evaluate the amount of transdermal water loss. The results are shown in Table 4 (the smaller the number of transdermal water loss, the better the effect).

The amount of percutaneous water loss due to differences in the raw material sample (water evaporating at 1 cm 2 of skin per unit time, g) Condition Immediately 10 minutes 20 minutes 30 minutes Control Cream 16.8 13.8 14.1 10.2 Raw material sample 1 Additive cream 15.5 13.5 13.9 9.7 Raw material sample 2 Additive cream 14.8 13.2 14.0 10.3 Raw material sample 3 Additive cream 12.4 10.3 8.2 5.9 Raw material sample 4 Additive cream 11.2 8.5 7.1 5.3

Referring to Table 4, while the cream of the raw material samples 1 and 2 containing the single strain fermented whey had a large loss of transdermal water, while the loss of transdermal water loss with the cream of the raw material samples 3 and 4 decreased with time And it is confirmed that the effect is increased as each plant extract is included.

Korea Microorganism Conservation Center (overseas) KCCM43226, KCCM43227 20160819

<110> B & B KOREA CO, LTD.          International GFC <120> Cosmetics comprising whey obtained by fermentation with Korean          type lactic acid bacteria complex for improving elasticity or          moisturizing <130> aba <160> 2 <170> KoPatentin 3.0 <210> 1 <211> 1473 <212> DNA <213> Lactobacillus pentosus <400> 1 nggccncana tcatgcagtc gaacgaactc tggtatngat tggtgcttgc atcatgattt 60 acatttgagt gagtggcgaa ctggtgagta acacgtggga aacctgccca gaagcggggg 120 ataacacctg gaaacagatg ctaataccgc ataacaactt ggaccgcatg gtccgagntt 180 gaaagatggc ttcggctatc acttttggat ggtcccgcgg cgtattagct agatggtggg 240 gtaacggctc accatggcaa tgatacgtag ccgacctgag agggtaatcg gccacattgg 300 gactgagaca cggcccaaac tcctacggga ggcagcagta gggaatcttc cacaatggac 360 gaaagtctga tggagcaacg ccgcgtgagt gaagaagggt ttcggctcgt aaaactctgt 420 tgttaaagaa gaacatatct gagagtaact gttcaggtat tgacggtatt tattgacggt 480 atttaaccag aaagccacgg ctnactacgt gccagcagcc cgcggtaata cgtaggtggc 540 aagcgntgtc cggatttatt gggcgtaaag cgagcgcagg cggtttttta agtctgatgt 600 gaaagccttc ggctcaaccg aagaagtgca tcggaaactg ggaaacttga gtgcagaaga 660 ggacagtgga actccatgtg tagcggtgaa atgcgtagat atatggaaga acaccagtgg 720 cgaaggcggc tgtctggtct gtaactgacg ctgaggctcg aaagtatggg tagcaaacag 780 gattagatac cctggtagtc cataccgtaa acgatgaatg ctaagtgttg gagggtttcc 840 gcccttcagt gctgcagcta acgcattaag cattccgcct ggggagtacg gccgcaaggc 900 tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga 960 agctacgcga agaaccttac caggtcttga catactatgc aaatctaaga gattagacgt 1020 tcccttcggg gacatggata caggtggtgc atggttgtcg tcagctcgtg tcgtgagatg 1080 ttgggttaag tcccgcaacg agcgcaaccc ttattatcag ttgccagcat taagttgggc 1140 actctggtga gactgccggt gacaaaccgg aggaaggtgg ggatgacgtc aaatcatcat 1200 gccccttatg acctgggcta cacacgtgct acaatggatg gtacaacgag ttgcgaactc 1260 gcgagagtaa gctaatctct taaagccatt ctcagttcgg attgtaggct gcaactcgcc 1320 tacatgaagt cggaatcgct agtaatcgcg gatcagcatg ccgcggtgaa tacgttcccg 1380 ggccttgtac acaccgcccg tcacaccatg agagtttgta acacccaaag tcggtggggt 1440 aacctttagg aaccagccgc ctatgtggtn ctg 1473 <210> 2 <211> 1477 <212> DNA <213> Unknown <220> <223> Lactobacillus bombicola <400> 2 ctgcatgggg ctgctataca tgcagtcgag cgagcaattt taacggaata ccttcgggta 60 ggaagataaa agcgcgagcg gcggatgggt gagtaacacg tgggcaacct accctttagc 120 ttgggatacc acttggaaac aggtgctaat accaaataag aagcaagagc gcatgctcaa 180 gctatgaaag gcggctttcg agctgtcact aaaggatggg cccgcggtgc attagctagt 240 tggtaaggta acggcttacc aaggcaatga tgcatagccg agttgagaga ctgatcggcc 300 acattgggac tgagacacgg cccaaactcc tacgggaggc agcagtaggg aatcttccac 360 aatggacgca agtctgatgg agcaacgccg cgtgagtgaa gaaggttttc ggatcgtaaa 420 gctctgttgt tggtgaagaa ggatagggat agtaactgat tcttatttga cggtaatcaa 480 ccagaaagtc acggctaact acgtgccagc agccgcggta atacgtaggt ggcaagcgtt 540 gtccggattt attgggcgta aagcgaacgc aggcgggaga acaagtcagc tgtgaaagcc 600 ctcggcttaa ccggggaagt gcagctgaaa ctgtttttct tgagtgcaga agaggagagt 660 ggaactccat gtgtagcggt ggaatgcgta gatatatgga agaacaccag tggcgaaggc 720 ggctctttgg tctgtaactg acgctgaggt tcgaaagcat gggtagcgaa caggattaga 780 taccctggta gtccatgccg taaacgatga gtgctaagtg ttgggaggtt tccgcctctc 840 agtgctgcag ctaacgcatt aagcactccg cctggggagt acgaccgcaa ggttaaaact 900 caaaggaatt gacgggggcc cgcaccagcg gtggagcatg tggtttaatt cgaagcaacg 960 cgaagaacct taccaggtct tgacatctag tgaaaagcct agagataggt aatacccttc 1020 ggggacacta agacaggtgg tgcatggctg tcgtcagctc gtgtcgtgag atgttgggtt 1080 aagtcccgca acgagcgcaa cccttattat tagttgccag cattaagttg ggcactctaa 1140 tgagactgcc ggtgacaaac cggaggaagg tggggacgac gtcaagtcat catgcccctt 1200 atgacctggg ctacacacgt gctacaatgg ttagtacaac gaggagcaag cctgtgaagg 1260 caagcgaatc tcttaaagct aatctcagtt cggattgcac tctgcaactc gagtgcatga 1320 agctggaatc gctagtaatc gcggatcagc atgccgcggt gaatacgttc ccgggccttg 1380 tacacaccgc ccgtcacacc atgagagtct gtaataccca aagccggtaa gataacctgc 1440 aaaggagtca gccgtctaat gtagacagag tttggcg 1477

Claims (5)

And a whey obtained by fermenting milk with a strain of Lactobacillus pentosus GFC03 ( Lactobacillus pentosus GFC03, accession number: KCCM 4326) and lactobacillus bombicola IB3 (accession number: KCCM 43227) Wherein the cosmetic composition is a cosmetic composition. The method according to claim 1,
Wherein the whey is a supernatant obtained by centrifuging the yogurt obtained by inoculating and culturing Lactobacillus pentosus GFC03 strain and Lactobacillus bovicola IB3 strain in milk. 3. The method of claim 2,
Wherein the culturing is carried out at 28 to 33 DEG C for 2 to 7 days. The method according to claim 1,
Wherein the composition comprises rosemary leaf extract, pomegranate extract, mangosteen bark extract, butterfly bean flower extract, donkey oil and milk protein extract. 5. The method of claim 4,
Wherein the composition further comprises shea butter, argentine tree kernel oil, olive oil and sunflower seed oil.

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