KR20160079979A - Cosmetic composition comprising of abalone mucus for skin wrinkle improvement or skin whitening - Google Patents
Cosmetic composition comprising of abalone mucus for skin wrinkle improvement or skin whitening Download PDFInfo
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- KR20160079979A KR20160079979A KR1020140190650A KR20140190650A KR20160079979A KR 20160079979 A KR20160079979 A KR 20160079979A KR 1020140190650 A KR1020140190650 A KR 1020140190650A KR 20140190650 A KR20140190650 A KR 20140190650A KR 20160079979 A KR20160079979 A KR 20160079979A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/987—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of species other than mammals or birds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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Abstract
TECHNICAL FIELD The present invention relates to a cosmetic composition, and more particularly, to a functional cosmetic composition for improving skin wrinkles or whitening comprising an abrasive-derived viscous liquid as an active ingredient. The overturned vaginal fluid of the present invention has an effect of improving wrinkles through hyaluronic acid degrading enzyme and elastase degrading enzyme activity; At the same time, since it has skin whitening effect through tyrosinase inhibitory activity, the composition of the present invention containing it as an effective ingredient can effectively improve wrinkles of the skin and can be usefully used as a whitening functional cosmetic composition.
Description
The present invention relates to a cosmetic composition, and more particularly, to a functional cosmetic composition for improving skin wrinkles or whitening, which contains an abrasive-derived mucilage (mucus material) as an active ingredient.
Everyone is healthy, and the desire and desire to look young and beautiful do not spare time and effort. Therefore, many people exercise, prevent and treat health through exercise and medicines, and many cosmetic products are produced and sold for the pursuit of beauty.
As interest in well-being increases with the recent improvement in income levels and lifestyle changes, consumers are more likely to prefer high-quality products using natural resources rather than chemically synthesized materials currently in use In accordance with the needs of such consumers, the development of functional cosmetics based on natural resources having pharmaceutical efficacy intermediate between cosmetics and pharmaceuticals such as functional cosmetics and functional foods has attracted attention in the cosmetics and health food market .
Among the natural materials that have been used so far are extracts of traditional Chinese medicine, vegetables, fruits, flowers, etc., and patents related to natural materials include lactic acid bacteria fermented rice (Korean patent 10-0552329), ginseng (Korean patent 10-0356720) (Korean Patent No. 10-1992-002109), peony (Korean Patent No. 10-1992-002111), safflower (Korean Patent No. 10-1997-021273), production method of hair mousse using natural herbal medicine materials (Korean Patent No. 10-2001-0074786 ), A cosmetic composition using natural herbal fermentation (Korean Patent No. 10-2003-0011470), a cosmetic composition containing a hot water extract of herbal medicine and a manufacturing method thereof (Korean Patent 10-2003-0028300), a lotus leaf extract / 10-2008-0030969), compositions containing germinated soybean extract (Korean Patent No. 10-2007-0075806), and whitening cosmetic compositions containing plant extracts (Korean Patent No. 10-2006-0087880).
As such, in the field of cosmetics, interest in cosmetics using natural materials has increased, and studies such as whitening, skin wrinkle improvement, antioxidation, moisturizing, and elasticity are vigorously active.
With respect to skin whitening, conventionally, substances having ascorbic acid, kojic acid, arbutin, hydroquinone, glutathione, derivatives thereof, or tyrosinase inhibiting activity have been used in combination with cosmetics or medicines. However, The use thereof is limited due to the whitening effect, the safety problem to the skin, the formulation and the stability of the cosmetic composition.
In order to maintain proper skin moisture, studies have been conducted to supply moisture from the outside or to prevent water loss from the body. In fact, many kinds of moisturizers having water retention ability have been developed, It is widely used in the area. However, due to abnormal epidermal cell division and differentiation, skin causes various skin diseases such as dryness of skin, atopy and psoriasis. Such diseases can be slightly alleviated by a conventional moisturizing agent having only water retention function , But it is difficult to expect fundamental healing.
On the other hand, abalone is a generic term for molluscs belonging to the abalone family, and it is abundant in minerals such as calcium and phosphorus, which live in the sea waters around clean reefs and use seaweed as a food. And it has the effects of skin beauty, nourishment, and postpartum cooking. In particular, it is rich in taurine components, so it has preventive effect on liver protection, fatigue recovery, myocardial infarction, etc. It protects liver and kidneys, cleanses eyes, opens stomach, regulates seawater and is effective for nourishment, headache and dizziness It has a high content of collagen, which stimulates cell regeneration and skin metabolism, which helps skin beauty. As a high quality seafood, it has characteristics that can not be compared with other seafood in terms of nutrition and taste. It is used as a healthy food in Korea, but it is not universal because it is expensive.
Accordingly, the present inventors have sought to find a natural substance having no side effects on wrinkles and improved skin whitening effect, and have examined their pharmacological effects by focusing on abalone-derived mucilages (mucous substances).
As a result, the overturned vaginal fluid of the present invention has a wrinkle-reducing effect through hyaluronidase and elastase-inhibiting activity; At the same time, has a skin whitening effect through tyrosinase inhibitory activity.
Accordingly, an object of the present invention is to provide a functional cosmetic composition which has no side effects on the living body and effectively has a skin wrinkle-improving or whitening effect.
In order to achieve the above object, the present invention provides a cosmetic composition for skin wrinkle improvement or whitening comprising a glycoprotein isolated from an abalone lipid vaginal fluid or an abalone lipid vaginal fluid as an active ingredient.
In one embodiment of the present invention, the abalone vaginal fluid secretes mucus from the abalone and then filters the milky liquid; And dialyzing the filtered viscous liquid and centrifuging to obtain a supernatant.
In one embodiment of the present invention, the rollover is a Haliotis discus hannai , H. gigantean , H. discus discus , H. sieboldii , and H. diversicolor superfextra .
In one embodiment of the present invention, the glycoprotein isolated from the abalone lipid vaginal fluid or the abalone vaginal fluid may be 0.0001 to 20% by weight based on the total weight of the composition.
In one embodiment of the invention, the composition is in the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray ≪ / RTI >
The overturned vaginal fluid of the present invention has an effect of improving wrinkles through hyaluronic acid degrading enzyme and elastase degrading enzyme activity; At the same time, since it has skin whitening effect through tyrosinase inhibitory activity, the composition of the present invention containing it as an effective ingredient can effectively improve wrinkles of the skin and can be usefully used as a whitening functional cosmetic composition.
Fig. 1 shows the results of analysis of constituent components of the polysaccharide in the abalone vaginal fluid of the present invention.
FIG. 2 shows the results of quantitative analysis of the glycoprotein components in the abalone of the present invention using FPLC (Fast Protein Liquid Chromatography) system and
FIG. 3 shows the result of analysis of the glycoprotein in the abalone of the present invention by SDS-page analysis.
FIG. 4 is a graph showing the measurement of hyaluronidase inhibitory activity (12.5, 25, 50, 100 v / v) of the glycoprotein concentration in the abalone lipid of the present invention.
FIG. 5 is a graph showing measurement of elastase inhibitory activity (12.5, 25, 50, 100 v / v) of the glycoprotein concentration in the abalone of the present invention.
The present invention is characterized by providing a cosmetic composition for skin whitening or wrinkle improvement comprising a glycoprotein isolated from an abalone or an abalone vaginal liquid as an active ingredient.
The 'mucus secretion' of the present invention is a mucous liquid synthesized by living organisms, which is also called mucilage or viscous substance, and is synthesized and secreted in the lineage cells in the epidermis of plants and some animals. These viscous liquids are known to contain glycoproteins, polysaccharides and inorganic salts.
Therefore, the 'abalone vaginal fluid' of the present invention means a mucous substance having a sticky and viscous secretion when stimulating the abalone. Preferably, the secretory vaginal fluid secreted by stimulating the abalone is filtered and then dialyzed to remove the odor. And finally centrifuged to obtain the supernatant.
That is, the abalone vaginal fluid of the present invention comprises the steps of secreting a mucus from an abalone and then filtering the mucus; And dialyzing the filtered viscous liquid and centrifuging to obtain a supernatant. At this time, it is possible to stimulate the abalone to secrete the mucus from the abalone. In order to stimulate the abalone, salt or solo The abalone may be rubbed, or live abalone may be put into hot water at 45-100 ° C to induce the stimulus momentarily. On the other hand, since the filtered viscous liquid has a characteristic odor, a dialysis process can be performed to remove the odor, and the supernatant obtained by centrifuging the dialyzed mucilaginous fluid is referred to as the " '.
The glycoprotein isolated from the abalone vaginal fluid of the present invention means a substance obtained by separating only the glycoprotein from the abalone vaginal fluid. The glycoprotein isolated from the abalone vaginal fluid of the present invention may be a conventional (Qiagen, Qproteom Mammalian Protein Prep Kit, Cat. No. 37901) in accordance with the manufacturer's instructions, in the following examples. Respectively.
The inventors of the present invention focused on abalone lipid vasicle and searched for a natural substance having excellent function such as skin wrinkle improvement and whitening effect without adverse effects on the living body, and found that the abalone lipid lipase (hyaluronic acid decomposing enzyme hyaluronidase) and elastase inhibitory activity; At the same time, has a skin whitening effect through tyrosinase inhibitory activity.
When the composition of the present invention is prepared with a cosmetic composition, the composition of the present invention may contain not only the above-mentioned abalone lipid (or glycoprotein separated from the abalone lipid), but also components commonly used in cosmetic compositions, Such as antioxidants, stabilizers, solubilizers, vitamins, customary adjuvants such as pigments and flavoring agents, and carriers.
In addition, the cosmetic composition of the present invention can be used as an anti-wrinkle agent or a skin care agent that has been conventionally used as long as it does not impair the skin protecting effect by reacting with the abalone vaginal fluid described above (or the glycoprotein separated from the abalone vaginal fluid) A whitening agent may be mixed and used.
In addition, the cosmetic composition of the present invention can be used not only in the above-mentioned abalone vaginal fluid (or glycoprotein isolated from the abalone vaginal fluid), but also in water-soluble vitamins, Vitamins, high molecular weight peptides, polymeric polysaccharides, sphingolipids and seaweed extracts.
The water-soluble vitamin is not particularly limited as long as it can be compounded in cosmetics. Preferably, vitamin B, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, And their salts (thiamine hydrochloride, sodium ascorbate, etc.) or derivatives (sodium ascorbic acid-2-phosphate, magnesium ascorbate-2-phosphate etc.) can also be added to water-soluble vitamins . The water-soluble vitamin can be obtained by a conventional method such as a microorganism conversion method, a purification method from a culture of a microorganism, an enzymatic method, or a chemical synthesis method.
As the fat-soluble vitamin, any of vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol) and the like can be mentioned, , Derivatives thereof (such as palmitic acid ascorbin, stearic acid ascorbic acid, dipalmitic acid ascorbin, dl-alpha tocopherol acetic acid, dl-alpha tocopherol nicotinic acid vitamin E, dl-pantothenyl alcohol, D-pantothenyl alcohol, Ether, etc.) are also included in the usable vitamins used in the present invention. The fat-soluble vitamin can be obtained by a conventional method such as a microorganism conversion method, a purification method from a culture of a microorganism, an enzyme, or a chemical synthesis method.
The polymeric peptide may be any compound as long as it can be compounded in cosmetics, and examples thereof include collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, and keratin. The polymeric peptide can be obtained by a conventional method such as purification from a culture broth of a microorganism, an enzymatic method, or a chemical synthesis method, or it can be purified from natural products such as ducks such as pigs and cows and silk fiber of silkworms.
The polymeric polysaccharide may be any compound as long as it can be incorporated in cosmetics, and examples thereof include hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.). For example, chondroitin sulfate or a salt thereof can be usually purified from mammals or fish.
Sphingo lipids may be any as long as they can be incorporated into cosmetics, and preferable examples thereof include ceramides, phytosphingosine and sphingoglycolipids. Sphingoid lipids can be purified from ordinary mammals, fish, shellfish, yeast or plants by conventional methods or can be obtained by chemical synthesis.
The seaweed extract may be any of those which can be compounded in cosmetics. Preferably, the seaweed extract is selected from the group consisting of algae extract, red pepper extract, green algae extract and the like. Also, the algae extract may be colored guanine, arginic acid, Potassium alginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained from seaweed by a conventional method.
The cosmetic composition of the present invention may be blended with other necessary ingredients, if necessary, in addition to the essential ingredients.
Examples of the compounding ingredients that may be added include organic solvents such as a preservative component, a moisturizer, an emollient, a surfactant, an organic and inorganic pigment, an organic powder, an ultraviolet absorbent, a preservative, a bactericide, an antioxidant, a plant extract, a pH adjuster, A blood circulation accelerator, a cold agent, an antiperspirant agent, and purified water.
Examples of the oil retaining component include ester-based oil retaining, hydrocarbon-based oil retaining, silicone-based oil retaining, fluoric oil retaining, animal retention and plant retention.
Examples of ester-based fats include glyceryl tri-2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isostearyl isostearate, Butyl isopropyl myristate, isopropyl myristate, isopropyl myristate, isopropyl myristate, isopropyl myristate, isopropyl myristate, butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isosilyl myristate, isostearic acid isostearyl, isostearyl palmitate, octyldodecyl myristate, Trimethylol propane, triisostearic acid trimethylol propane, tetra 2-ethylhexanoic acid pentaerythritol tetra (2-ethylhexanoate) , Decyl caprylate, decyl laurate, hexyl laurate, myristate decyl, myristyl myristate, myristine monoethyl stearate, stearyl stearate, decyl oleate, ricinoleic acid tri Isopropyl palmitate, isostearyl stearate, isostearyl stearate, isodecyl oleate, octyldodecyl oleate, octyldodecyl linoleate, isopropyl stearate, isopropyl stearate, isopropyl stearate, Ethylhexanoate, stearyl 2-ethylhexanoate, stearyl 2-ethylhexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicaprate, di (capryl, capric acid) propylene glycol, Propylene glycol dicaprylate, propylene glycol dicaprylate, neopentyl glycol dicaprate, neopentyl glycol dioctanoate, glyceryl tricarboxylate, glyceryl triunsaturated acid, glyceryl triisopalmitate, glyceryl triisostearate, octyl dodecanoate Silane, octanoic acid, octanoic acid, octanoic acid, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, Octyldecyl lactate, octyldecyl lactate, octyldecyl lactate, polyglycerin oleic acid ester, polyglycerin isostearic acid ester, triisocetyl citrate, triisobutyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, But are not limited to, ethyl, acetyltriethyl citrate, acetyltributyl citrate, trioctyl citrate, diisostearyl malate, 2-ethylhexyl hydroxystearate, di-2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, But are not limited to, dioctyl sebacate, stearic acid cholesteryl, isostearic acid cholesteryl, hydroxystearic acid cholesteryl, oleic acid cholesteryl, oleic acid dihydrocholesteryl, isostearic acid pitostearyl, Stearyl hydroxystearic acid isostearyl, 12-stearoyl hydroxystearic acid stearyl, 12-stearoyl hydroxystearic acid stearyl, One hydroxy stearic acid and the like esters such as cetearyl source.
Examples of the hydrocarbon hydrocarbon-based fats include hydrocarbon fats and oils such as squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, floating isoparaffin, polybutene, microcrystalline wax and vaseline.
Examples of silicone based oils include polymethyl silicone, methylphenyl silicone, methyl cyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane-methylcetyloxysiloxane copolymer, dimethylsiloxane-methylstarchoxysiloxane copolymer, alkyl Modified silicone oils, and amino-modified silicone oils.
Examples of the fluorine-based oil include perfluoropolyether and the like.
Examples of animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, new flower oil, soybean oil, corn oil, rape oil, apricot kernel oil, palm kernel oil, palm oil, castor oil, , Corn oil, palm oil, palm oil, cucumber nut oil, wheat germ oil, rice germ oil, shea butter, coltsfoot colostrum, marker daisy nut oil, mead home oil, egg oil, , Canned wax, carnauba wax, liquid lanolin, hardened castor oil, and the like.
Examples of the moisturizing agent include water-soluble low-molecular moisturizing agents, oil-soluble molecular moisturizing agents, water-soluble polymers, and oil-soluble polymers.
Examples of the water-soluble low-molecular moisturizing agent include serine, glutamine, sorbitol, mannitol, sodium pyrrolidone-carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B Glycol (polymerization degree n = 2 or more), polyglycerin B (polymerization degree n = 2 or more), lactic acid, lactic acid salt and the like.
Examples of the lipid-soluble low-molecular moisturizing agent include cholesterol and cholesterol ester.
Examples of the water-soluble polymer include carboxyvinyl polymer, polyaspartic acid, tragacanth, xanthan gum, methylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, carboxymethylcellulose, water-soluble chitin, chitosan, dextrin, etc. .
Examples of the oil-soluble polymer include polyvinylpyrrolidone / eicosene copolymer, polyvinylpyrrolidone / hexadecene copolymer, nitrocellulose, dextrin fatty acid ester, and polymer silicone.
Examples of the emollients include long chain acyl glutamic acid cholesteryl ester, hydroxystearic acid cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid and lanolin fatty acid cholesteryl ester.
Examples of the surfactant include nonionic surfactants, anionic surfactants, cationic surfactants, and amphoteric surfactants.
Examples of the nonionic surfactant include self emulsifying monostearate glycerin, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbit fatty acid ester, POE (Polyoxyethylene / polyoxypropylene) copolymer, POE.POP alkyl ether, polyether-modified silicone, polyether-modified silicone, polyoxyethylene-polyoxypropylene (POE) Alkanolamides, alkylamine oxides, hydrogenated soybean phospholipids, and the like.
Examples of the anionic surfactant include fatty acid soap, alpha-acylsulfonate, alkylsulfonate, alkylarylsulfonate, alkylnaphthalenesulfonate, alkylsulfate, POE alkyl ether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl ginseng salt, Alkylsulfosuccinic acid salts, acylated hydrolyzed collagen peptide salts, and perfluoroalkyl phosphoric acid esters, and the like can be mentioned. have.
Examples of the cationic surfactant include alkyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium bromide, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyl dimethyl benzyl ammonium chloride, behenyl trimethyl ammonium chloride, Benzalkonium, diethylaminoethylamide stearate, dimethylaminopropylamide stearate, quaternary ammonium salts of lanolin derivatives, and the like.
Examples of the amphoteric surfactant include carboxybetaine type, amide betaine type, sulfobetaine type, hydroxysulfobetaine type, amidosulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type and amide amine type Amphoteric surfactants and the like.
Examples of the organic and inorganic pigments include inorganic pigments such as silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, Bengala, clay, bentonite, titanium mica, titanium oxide, bismuth chloride, zirconium oxide, magnesium oxide, Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, chromium oxide, chromium oxide, chromium hydroxide, But are not limited to, polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluororesin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, Silk powder, cellulose, CI Pigment Yellow, CI Pigment Orange, and composite pigments of inorganic pigments and organic pigments thereof.
As the organic powder, metallic soap such as calcium stearate; Metal salts of alkyl phosphates such as sodium zinc cetylate, zinc laurylate and calcium lauryl laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc and N-lauroylglycine calcium; Amidosulfonic acid multivalent metal salts such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; Such as N-epsilon-lauroyl-L-lysine, N-epsilon-palmitoylidene, N-alpha-paratyylnitine, N-alpha-lauroyl arginine, Acyl basic amino acids; N-acylpolypeptides such as N-lauroylglycylglycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid, alpha-aminoaurauric acid, and the like; Polyethylene, polypropylene, nylon, polymethylmethacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride, and the like.
Examples of ultraviolet absorbers include paraaminobenzoic acid, ethyl parnamobenzoate, amyl paranobenzoate, octyl paranobenzoate, ethyleneglycol salicylate, phenyl salicylate, benzyl salicylate, benzyl salicylate, butylphenyl salicylate, homomenthyl salicylate, benzyl cinnamate , Octyl methoxycinnamate, dioctyl methoxycinnamate, mono-2-ethylhexane glyceryl dipyrromethoxycinnamate, isopropyl paratumoxycinnamate, diisopropyl-diisopropyl cinnamate ester mixture, Carninoic acid, ethyl urocanoate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenone sulfonic acid and salts thereof, dihydroxymethoxybenzophenone, sodium dihydroxymethoxybenzophenone disulfonate, dihydroxybenzophenone , Tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianylino-p- (carbo-2'-ethylhexyl- , 3,5-triazine, 2- (2-hydroxy Methyl-5-methylphenyl) benzotriazole, and the like.
Examples of the disinfectant include hinokitiol, trichloroacid, trichlorohydroxydiphenyl ether, crohexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, zinc filitione, benzalkonium chloride, No. 301, mononitro and eicol sodium, and undecylenic acid.
Examples of the antioxidant include butylhydroxyanisole, gallic acid propyl, and eicosorbic acid.
Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fumaric acid, sodium fumarate, succinic acid, sodium succinate, sodium hydroxide, sodium monohydrogenphosphate and the like.
Examples of the alcohol include higher alcohols such as cetyl alcohol.
The cosmetic composition of the present invention may take the form of a solution, an emulsion, a viscous mixture or the like.
Examples of products to which the cosmetic composition of the present invention can be added include cosmetics such as astringent lotion, softening longevity lotion, nutrition lotion, various creams, essences, packs, foundation and the like, cleansing, cleanser, soap, .
As a specific formulation of the cosmetic composition of the present invention, there may be mentioned a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, It includes formulations such as soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, latex, lipstick, makeup base, foundation, press powder, loose powder, eye shadow.
When the formulation of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .
In the case where the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, a mixture of chlorofluorohydrocarbons, propane / Propane or dimethyl ether.
In the case of the solution or emulsion of the present invention, a solvent, a solvent or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.
When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.
When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolenic derivatives or ethoxylated glycerol fatty acid esters.
According to a preferred embodiment of the present invention, the content of the overturned vaginal fluid (or the glycoprotein isolated from the abalone vaginal fluid) of the present invention is 0.0001-20% by weight, preferably 0.5-20% More preferably 1.0 to 20% by weight. If the content of the overturned vaginal fluid (or the glycoprotein isolated from the abalone vaginal fluid) is less than 0.0001 wt%, the wrinkle improvement or skin whitening effect due to the abalone vaginal fluid (or the glycoprotein isolated from the abalone vaginal fluid) If it exceeds 20% by weight, skin irritation may be caused, and problems of the shape may occur.
On the other hand, the cosmetic composition according to the present invention may be formulated by incorporating the abalone vaginal fluid (or the glycoprotein isolated from the abalone vaginal fluid) in the nanoliposome and stabilizing it. When the above abalone vaginal fluid (or the glycoprotein isolated from the abalone vaginal fluid) is contained in the nanoliposome, the components of the abalone vaginal fluid (or the glycoprotein isolated from the abalone vaginal fluid) are stabilized, And the solubility and transdermal absorption rate of the component can be increased. Thus, it is possible to maximize the expected efficacy from the abalone vaginal fluid (or the glycoprotein isolated from the abalone vaginal fluid).
In the present invention, nanoliposome refers to a liposome having a typical liposome form and having an average particle diameter of 10 to 500 nm. According to a preferred embodiment of the present invention, the average particle diameter of the nanoliposome is 50 to 300 nm. When the average particle diameter of the nanoliposome is more than 300 nm, improvement of skin penetration and improvement of formulation stability is very weak among technological effects to be achieved in the present invention.
The nanoliposomes used to stabilize the abalone vaginal fluid (or the glycoprotein isolated from the abalone vaginal fluid) according to the present invention may be prepared by a mixture comprising a polyol, an oily component, a surfactant, a phospholipid, a fatty acid and water .
The polyol used in the nanoliposome of the present invention is not particularly limited and is preferably a polyol such as propylene glycol, dipropylene glycol, 1,3-butylene glycol, glycerin, methylpropanediol, isopropylene glycol, pentylene glycol, erythritol, , Sorbitol, and mixtures thereof. The amount thereof is 10 to 80% by weight, preferably 30 to 70% by weight, based on the total weight of the nanoliposome.
The oil component used in the preparation of the nanoliposome of the present invention may be selected from a variety of oils known in the art and is preferably a hydrocarbon oil such as hexadecane and paraffin oil, Vegetable oils such as silicon oil such as dimethicone and cyclomethicone, sunflower oil, corn oil, soybean oil, avocado oil, sesame oil and fish oil, ethoxylated alkyl ether oils, propoxylated alkyl ether oils 40 is a fatty alcohol, and mixtures thereof -, phytosphingosine, sphingosine, and scan non-ping the same scan pinggo cannabinoid lipid, cholesterol side-by celebrity, sitosterol cholesteryl sulfate, cholesteryl sulfate, cytokines, C 10. The amount thereof may be 1.0 to 30.0% by weight, and preferably 3.0 to 20.0% by weight based on the total weight of the nanoliposome.
Any surfactant known in the art may be used as the surfactant used in the preparation of the nanoliposome of the present invention. For example, anionic surfactants, cationic surfactants, amphoteric surfactants and nonionic surfactants can be used. Preferred are anionic surfactants and nonionic surfactants. Specific examples of anionic surfactants include alkyl acyl glutamates, alkyl phosphates, alkyl lactylates, dialkyl phosphates and trialkyl phosphates. Specific examples of nonionic surfactants include alkoxylated alkyl ethers, alkoxylated alkyl esters, alkyl polyglycosides, polyglyceryl esters and sugar esters. Particularly preferred surfactants are polysorbates belonging to nonionic surfactants. The amount thereof may be 0.1 to 10% by weight, preferably 0.5 to 5.0% by weight, based on the total weight of the nanoliposome.
The phospholipid, another component used in the preparation of the nanoliposomes of the present invention, is a lipophilic lipid that is used with both affinity lipids and is composed of natural phospholipids (e.g., egg yolk lecithin or soy lecithin, sphingomyelin) Dipalmitoyl phosphatidylcholine or hydrogenated lecithin), preferably lecithin. In particular, unsaturated lecithin or saturated lecithin derived from natural origin extracted from soybean or egg yolk is preferable. Normally, the amount of phosphatidylcholine is 23 to 95% and the amount of phosphatidylethanolamine is 20% or less in natural derived lecithin. In the production of the nanoliposome of the present invention, the amount of the phospholipid to be used is 0.5 to 20.0% by weight, preferably 2.0 to 8.0% by weight based on the total weight of the nanoliposome.
Fatty acid to be used in nano-liposome preparation of the present invention is a higher fatty acid, preferably a C 12 - 22 as a saturated or unsaturated fatty acid of the alkyl chain, e.g., lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid and linoleic acid . The amount thereof may be 0.05 to 3.0% by weight, preferably 0.1 to 1.0% by weight, based on the total weight of the nanoliposome.
The water used in the preparation of the nanoliposome of the present invention is generally deionized distilled water, and the amount thereof may be 5.0-40 wt% based on the total weight of the nanoliposome.
The preparation of nanoliposomes can be accomplished through a variety of methods known in the art, but most preferably is made by applying a mixture comprising the ingredients to a high pressure homogenizer. The preparation of a nanoliposome by a high pressure homogenizer can be carried out under various conditions (for example, pressure, number of times, etc.) depending on a desired particle size, and preferably at a high pressure homogenizer So that the nanoliposome can be produced.
The cosmetic composition for skin wrinkle improvement or whitening according to the present invention may contain 0.1 to 20.0% by weight based on the weight of the nanoliposome impregnated with an abalone lipid (or glycoprotein isolated from abalone lipid) as an active ingredient.
Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are for further illustrating the present invention, and the scope of the present invention is not limited to these examples.
< Example 1>
The abalone of the present invention Point vaginal Manufacturing method
The inventors of the present invention prepared an abalone vaginal fluid of the present invention which can finally be used as a raw material for cosmetics after dialysis and centrifugation of secreted vaginal fluid through the stimulation of abalone to extract the vaginal vaginal fluid from the abalone.
First, the following two methods were used to secrete mucus from abalone.
As a first method, when the abalone is purchased on the market, when the abalone scaffold and the black band and the abscissa of the abalone frame are rubbed with salt or solo abalone to remove the abalone, the abalone is stimulated to secrete the mucus secretion. The viscous liquid was filtered with a filter cloth to obtain a viscid liquid. As a second method, live abalone purchased from the market is put into hot water at a temperature of 45-100 ° C and momentarily stimulated to secrete the mucus secretion. The mucous secretion secreted at this time is filtered with a filter cloth to obtain a mucus secretion.
To remove the characteristic smell, 40 ml of mucilage was dialyzed in 1600 ml of phosphate buffered saline (PBS) buffer at 4 ° C for 5 days (Spectrum Laboratories, Inc., Spectra / por Dialysis Membrane MWCO: 12-14,000) and centrifuged at 13,500 rpm for 5 minutes at room temperature to obtain supernatant.
The obtained supernatant was named " abalone vaginal fluid " of the present invention and used as a sample for the following experiment in order to confirm the possibility of its functional cosmetic material (raw material).
< Example 2>
The abalone of the present invention Within the vagina Polysaccharide Per constitution analysis
In order to analyze the constituent sugars of the polysaccharides in the abalone vaginal fluid prepared in Example 1, the present inventors analyzed the constituents of the polysaccharides after purifying the polysaccharides contained in the abalone vaginal fluid.
<2-1> Abalone From the viscous liquid Purification of polysaccharides
Ethanol (95% ethanol in three times the amount of the vaginal solution) was added to 20 ml of the abalone of the present invention prepared in Example 1, washed with 95% ethanol three times, dissolved in distilled water, dialyzed, The polysaccharide was separated and purified from the abalone vaginal fluid.
≪ 2-2 > Per constitution analysis
For the constitutional analysis of polysaccharide derived from abalone-derived vaginal liquid, the sample prepared through the procedure of Example <2-1> was added to 4M TFA (trifluoroacetic acid) in a volume ratio of 1: 2 and reacted at 100 ° C for 3 hours And then completely hydrolyzed. After drying, it was dissolved in distilled water using a vacuum dryer. After drying in a vacuum dryer, the thus-prepared monosaccharide sample was dissolved in purified water, and then finally purified using a CarboPac PA1 column to obtain HPAEC- PAD (High Performance Anion-Exchage Chromatography with Pulsed Amperometric Detection; Bio-LC). The optimal analysis conditions for the Bio-LC analysis are shown in FIG. 1, wherein A in FIG. 1 refers to the reference material and B refers to the abalone vaginal fluid of the present invention.
As a result, as shown in Fig. 1, the polysaccharides present in the abalone-depoactant of the present invention are glucose (Glc), galactose (Gal), rhamnose (Rha), glucosamine (GlcN), fucose (Fuc), mannose ) And polysaccharide component. Major constituents were Glc, Gal, GlcN and Rha. In addition, a small amount of unknown substance was detected, which is presumed to be a minor mineral component. Generally, in extracting useful constituents from marine-derived organisms, crude extract is carried out using diluted acid in order to remove trace minerals other than calcium carbonate and calcium phosphate. However, in this experiment, it is considered that the trace minerals contained in the mucus are not removed because the mucus obtained by rapid thermal stress on the abalone was simply dialyzed and only the supernatant was taken.
< Example 3>
The abalone of the present invention Within the vagina Content analysis of glycoprotein
The present inventors used a glycoprotein separation kit (Qiagen, Qproteome Mammalian Protein Prep Kit, Cat. No. 37901) to analyze the content of glycoproteins in addition to the polysaccharide component in the abalone lipid prepared according to the above Example 1 of the present invention. ≪ / RTI > according to the manufacturer ' s instructions.
Specifically, 20 ul of the abalone-derived viscous supernatant was loaded onto a 1.5 ml tube filled with a resin having glycoprotein-specific affinity, and centrifuged at 500 rpm for 5 minutes. After centrifugation, PBS was loaded into the tube to remove impurities other than glycoprotein bound to the resin in the tube, followed by centrifugation twice. Finally, to collect the glycoprotein bound to the resin, a glycoprotein-binding buffer with a higher glycoprotein-specific affinity than the resin packed in the tube was loaded into the tube and centrifuged as described above, The glycoproteins derived from the supernatant were collected. The content of glycoproteins derived from abalone-derived vaginal fluid was determined by separating the glycoprotein of the viscous supernatant before and after the passage through the kit using the FPLC system and the
As a result, it was confirmed that the content of glycoprotein was about 35% (see FIG. 2). Further, it was confirmed by SDS-PAGE that the main product was a glycoprotein having a molecular weight of 180-kDa 3).
< Example 4>
Abalone of the present invention Viscous Whitening activity
The inventors of the present invention conducted experiments to evaluate whether the abalignment vaginal fluid of the present invention prepared in Example 1 has a whitening activity function as a raw material of cosmetics, 3 glycoprotein were examined for tyrosine enzyme inhibitory activity.
Because tyrosinase is a kind of oxidase, it is known as an enzyme that regulates melanin production, so it can reduce the accumulation of melanin in the body by inhibiting this enzyme. To measure enzyme inhibitory activity of tyrosinase, L-DOPA (dihydroxyphenyalanine), one of the precursors of melanin, was used as a substrate and tyrosinase from mushroom (Sigma) was used as tyrosinase. The aqueous solution of L-DOPA itself is transparent but pinkish in a time-dependent manner by tyrosinase enzyme activity. Using these characteristics, L-DOPA was prepared at concentrations ranging from 0 to 2% (w / v) in Tris-Cl buffer (pH 7.2) and mushroom-derived tyrosinase was added to the substrate to a final concentration of 5 ug / And allowed to react at room temperature for 2 minutes. The reaction mixture was boiled at 100 DEG C for 5 minutes to stop the enzyme reaction, and the denatured protein was precipitated through centrifugation, and the supernatant was obtained. The obtained supernatant was loaded into 96 wells and absorbance at 400 nm was obtained to obtain a calibration curve. Based on the obtained calibration curve, the tyrosinase enzyme inhibitory activity of the glycoprotein derived from abalone vaginal epithelium was calculated as a relative activity.
* Inhibition rate (%) = (1 - absorbance of sample addition group / absorbance of no addition group) x 100
As a result, the tyrosinase inhibitory activity of the glycoprotein derived from the abalone of the present invention was found to be 35%, indicating that there was whitening activity (not shown).
< Example 5>
Abalone of the present invention Viscous Wrinkle improving activity
In order to evaluate whether or not the abalignment vaginal fluid of the present invention produced through Example 1 has a wrinkle-improving active function as a raw material (material) for cosmetics, the inventors of the present invention conducted the above- Enzyme inhibitory activity against hyaluronic acid degrading enzyme and elastin degrading enzyme was investigated in the glycoprotein of Example 3.
In order to compare the activity values according to the concentration, ethanol was diluted with 68% ethanol at 100% concentration of the supernatant obtained after ethanol precipitation. In order to consider the effect of ethanol on the enzyme reaction, 68% The values were also measured.
In order to measure the enzyme inhibitory activity against hyaluronic acid degrading enzyme and elastin degrading enzyme, the concentration of enzyme and substrate suitable for the experiment was determined as shown below by a calibration curve.
In the case of hyaluronic acid degrading enzyme inhibitory activity, 50 μl of hyaluronidase from bovine testase (7900 U / ml, acetate buffer pH 5.35) and 12.5 μl of hyaluronidase purchased from Sigma Aldrich Co. 100 μl of mM CaCl 2 (activator) was reacted at 37 ° C. for 5 minutes, and then 100 μl of the viscous supernatant and 250 μl of 0.12% hyaluronic acid were added. After the reaction mixture was reacted at 37 ° C for 10 minutes, the enzyme reaction was stopped by adding 0.4 N NaOH, and the amount of N-acetylglucosamine derived from hyaluronic acid contained in the supernatant obtained after centrifugation was measured at 535 nm. The relative activity was calculated by comparing with the control group. The elastase from human leukocytes, 6.6 ug / ml, sodium phosphate buffer, pH (pH), and phosphate buffered saline (PBS) were prepared using the elastase purchased from Sigma-Aldrich Co. 6.5) and 0.012 mg / ml N-suc- (Ala) 3-p-nitroanilide was used as the substrate. The supernatant was added and incubated for 10 minutes at 37 ° C. Absorbance was measured at 405 nm. The measured absorbance was compared with the control to calculate the relative activity.
As a result, as shown in Fig. 4 and Fig. 5, the maximum inhibitory activity of the glycoprotein isolated from the abalone of the present invention to hyaluronic acid degrading enzyme was 57% (see Fig. 4), and the elastin degrading enzyme And the maximum inhibitory activity was 40% (see FIG. 5).
From the above results, it was found that the abalone vaginal juice of the present invention is excellent in whitening activity and wrinkle improvement effect, and the abalone vaginal juice will be useful as a cosmetic raw material having whitening and wrinkle improving function .
< Example 6>
abalone
Mouth juice
For skin whitening and wrinkle improvement
Cosmetics
Composition manufacturing
<6-1> Flexible longevity skin )
The softened skin containing the abalone lipid of the present invention prepared (prepared) through Example 1 was prepared in a conventional manner at the following composition ratios.
(Unit: wt%)
(Unit: wt%)
<6-2> Nourishing lotion ( lotion )
A lotion containing the abalone cream of the present invention prepared (manufactured) through Example 1 was prepared in a conventional manner at the following composition ratios.
(Unit: wt%)
(Unit: wt%)
<6-3> Nourishing cream
A nutritive cream containing the abalone cream of the present invention prepared (manufactured) through Example 1 was prepared in a conventional manner at the following composition ratios.
(Unit: wt%)
(Unit: wt%)
<6-4> Pack
A pack containing the abalone of the present invention (prepared) prepared in Example 1 above was prepared in a conventional manner at the following composition ratios.
(Unit: wt%)
(Unit: wt%)
< Example 7>
Stability test of formulation
For the formulation stability test of the cosmetic composition of the present invention, the cosmetic composition formulated in Example 6 was placed in an opaque glass container and kept in a constant temperature bath maintained at 45 DEG C for 12 weeks. The degree of discoloration was measured after keeping for 12 weeks in a constantly kept, fully shaded refrigerator. The degree of discoloration of the product is classified into the following six grades (0: no change, 1: very little separation (discoloration), 2: little separation (discoloration), 3: (Discoloration)).
As a result, as shown in Table 5, the cosmetic composition of Production Example did not cause discoloration and separation phenomenon, and the stability (stability) of the formulation of the cosmetic preparation containing the submerged mucilage prepared in It can be seen that there is no abnormality.
< Example 8>
Skin safety experiment
In order to test skin safety for the composition of the present invention, the following safety test was conducted on the cosmetic composition formulated in Example 6 above.
<8-1> Primary skin irritation test
The cosmetic preparation was applied to the skin of male white rabbits with hair on the dorsal skin removed once a day, five times a week to a thickness of 2 mg / cm 2 or more. The application was carried out for 2 weeks, and hair removal was carried out every week at the final application day. The test was performed at 24, 48, and 72 hours after application with the primary irritation test using erythema and edema as an index. As a result of the test, it was judged to be negative because no erythema and edema were observed in all experimental groups.
<8-2> Skin Cumulative Irritation Test
The cosmetic preparation was applied to the skin of male white rabbits with hair on the dorsal skin removed once a day, five times a week to a thickness of 2 mg / cm 2 or more. The application was carried out for 2 weeks, and hair removal was carried out every week at the final application day. The determination was carried out using the erythema and edema as the indicators by the primary irritation method on the day of each application and the day after the last application. As a result of the test, skin irritation caused by repeated application of the preparation was not observed in all the test groups and it was judged as negative.
The present invention has been described with reference to the preferred embodiments. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Therefore, the disclosed embodiments should be considered in an illustrative rather than a restrictive sense. The scope of the present invention is defined by the appended claims rather than by the foregoing description, and all differences within the scope of equivalents thereof should be construed as being included in the present invention.
Claims (5)
Secreting the vaginal fluid from the abalone and filtering the abalone vaginal fluid; And dialyzing the filtered viscous liquid and centrifuging to obtain a supernatant liquid.
The abalone is called Haliotis discus characterized in that it is at least one selected from the group consisting of hannai , H. gigantean , H. discus discus , H. sieboldii and H. diversicolor superfextra . Composition.
Wherein the glycoprotein isolated from the abalone lipid vaginal fluid or the abalone lipid lipid is contained in an amount of 0.0001 to 20% by weight based on the total weight of the composition.
The composition may be in the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray ≪ / RTI >
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| Application Number | Priority Date | Filing Date | Title |
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| KR1020140190650A KR20160079979A (en) | 2014-12-26 | 2014-12-26 | Cosmetic composition comprising of abalone mucus for skin wrinkle improvement or skin whitening |
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| KR1020140190650A KR20160079979A (en) | 2014-12-26 | 2014-12-26 | Cosmetic composition comprising of abalone mucus for skin wrinkle improvement or skin whitening |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102018205249A1 (en) * | 2018-04-09 | 2019-10-10 | Beiersdorf Ag | Cosmetic cleansing preparation containing mucin |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20100066822A (en) | 2008-12-10 | 2010-06-18 | (주)아모레퍼시픽 | Composition for skin external application containing for improving skin wrinkle or skin whitening |
| KR20140013795A (en) | 2012-07-27 | 2014-02-05 | 강원대학교산학협력단 | Cosmetic composition comprising of gingenoside f2 for skin wrinkle improvement, skin whitening or anti-acne |
| KR20140091311A (en) | 2013-01-11 | 2014-07-21 | 재단법인 한국한방산업진흥원 | A Method for producing a purified extract containing abundant amoun of tanshinone I and tanshinone IIA showing anti-oxidative activity, skin whitening activity and anti-wrinkle activity from Salvia miltiorrhiza BUNGE |
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2014
- 2014-12-26 KR KR1020140190650A patent/KR20160079979A/en not_active Application Discontinuation
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20100066822A (en) | 2008-12-10 | 2010-06-18 | (주)아모레퍼시픽 | Composition for skin external application containing for improving skin wrinkle or skin whitening |
| KR20140013795A (en) | 2012-07-27 | 2014-02-05 | 강원대학교산학협력단 | Cosmetic composition comprising of gingenoside f2 for skin wrinkle improvement, skin whitening or anti-acne |
| KR20140091311A (en) | 2013-01-11 | 2014-07-21 | 재단법인 한국한방산업진흥원 | A Method for producing a purified extract containing abundant amoun of tanshinone I and tanshinone IIA showing anti-oxidative activity, skin whitening activity and anti-wrinkle activity from Salvia miltiorrhiza BUNGE |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102018205249A1 (en) * | 2018-04-09 | 2019-10-10 | Beiersdorf Ag | Cosmetic cleansing preparation containing mucin |
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