KR102283637B1 - High functional cosmetic composition comprising the rind fermentation product - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising a Galactomyces pericarp fermented product which is obtained by fermenting pericarp using a Galactomyces sp. strain so as to contain a large amount of low molecular weight pectin, thereby having various skin beauty improvement effects. The cosmetic composition according to the present invention comprises: 0.1-10 parts by weight of a Galactomyces pericarp fermented product; 1-40 parts by weight of an additive including at least one selected from the group consisting of vitamins, peptides, polymer polysaccharide, sphingolipids, an oil and fat component, a moisturizer, an emollient agent, surfactants, paint, organic powder, a filler, an UV absorber, a preservative, a sterilizer, an antioxidant, a plant extract, a pH regulator, alcohol, a pigment and a fragrance; and 50-98.9 parts by weight of water (H_2O).

Description

High functional cosmetic composition comprising the rind fermentation product

The present invention relates to a cosmetic composition comprising a fermented product of Galactomyces peel, which is fermented by Galactomyces sp. strain and contains a large amount of low-molecular-weight pectin, and exhibits various skin beauty improvement effects.

In addition, the present invention relates to a cosmetic composition that further comprises a fermented product of Bifidobacterium seaweed and exhibits improved skin beauty improvement efficacy.

The skin acts as the body's primary barrier and protects internal organs from external environmental stimuli such as changes in temperature and humidity, UV rays, and pollutants. However, internally, as we age, the secretion of various hormones that control metabolism decreases, the function of immune cells and the activity of cells decrease, so that the biosynthesis of immune proteins and body-constituting proteins necessary for the living body decreases, and externally Due to the physical and chemical stimulation and stress caused by the increase of ultraviolet rays, free radicals and active oxygen caused by environmental pollution such as ozone layer depletion, the skin's cleansing function is weakened and aging is accelerated.

In order to maintain healthy and beautiful skin, various methods are being researched and developed to maintain the unique function of the skin by adding physiologically active substances derived from natural products obtained from animals, plants, and microorganisms to cosmetics. Various fermentation technologies are introduced and utilized to reduce the molecular weight of active ingredients to enhance skin beauty improvement performance by introducing microorganisms into the skin to promote the introduction of active ingredients into the skin.

On the other hand, pectin is a major component of plant cell walls and exists in cell membranes or thin layers between cell membranes of fruits and vegetables, etc. It is a substance (αD-1,4-polygalacturonic acid).

The industrial use of pectin is very limited, but it is mainly used for very limited uses such as thickeners and gel formers in the food field, and its use is extremely limited in the cosmetic field, which has physical and chemical disadvantages, compatibility, etc. of pectin is due to the problem of

Specifically, pectin is a high-viscosity water-soluble polysaccharide component, and the viscosity changes due to salt and pH, etc., and has a characteristic of gelatinization when mixed with alcohol, so that cosmetic application is not easy. In addition, due to the hydrophilic high molecular weight molecular structure of pectin, it is difficult to permeate through the skin, so it is difficult to show various physiological activities expected through oral administration through transdermal application, which is a form of cosmetic use.

Conventionally, a strong acid solution is added to pectin, irradiated, or macerozyme R-10, Pectinex Ultra SPL, Pectinex 3XL, Pectinex Y Various methods for adding pectin with low molecular weight to cosmetics by adding pectinase such as -23 (Pectolyase Y-23) to decompose pectin are being studied.

However, the method of irradiating radiation requires expensive equipment and facilities, it is difficult to obtain a decomposition product of a uniform molecular weight, such as a chemical decomposition method using acid treatment or enzyme treatment, and there is a problem that the manufacturing cost is greatly increased. Research on ways to complement it is needed.

Korean Patent Registration No. 10-1220091 (published on: May 31, 2012) Korean Patent Registration No. 10-1516198 (Announcement Date: 2015.05.18) Korean Patent Publication No. 10-2012-0127634 (published on: November 22, 2012)

The present invention has been devised to solve the problems of the prior art as described above, and Galactomyces peel fermentation is performed using a Galactomyces spp. It is intended to provide technical details regarding a cosmetic composition containing water.

In addition, the present invention relates to a cosmetic composition comprising a fermented product of Bifidobacterium seaweed with improved skin beauty improvement effect by fermenting seaweed using Bifidobacterium sp. It is intended to provide technical content.

In addition, an object of the present invention is to provide technical details regarding a cosmetic composition comprising an active ingredient derived from a natural material that is harmless to the human body, such as a fermented fruit skin product and a fermented seaweed product.

The technical problems to be solved by the present invention are not limited to the technical problems mentioned above, and other technical problems not mentioned are clearly understood by those of ordinary skill in the art to which the present invention belongs from the following description it could be

In order to achieve the technical problem as described above, the present invention, Galactomyces pericarp fermented product 0.1 to 10 parts by weight; Vitamins, peptides, polysaccharides, sphingolipids, oils and fats, humectants, emollients, surfactants, pigments, organic powders, fillers, UV absorbers, preservatives, fungicides, antioxidants, plant extracts, pH regulators, alcohols, pigments and fragrances 1 to 40 parts by weight of an additive comprising at least one selected from the group consisting of; And water (H ₂ O) 50 to 98.9 parts by weight; provides a cosmetic composition comprising.

In addition, the fermented Galactomyces peel may be prepared by fermenting one or more peels selected from the group consisting of banana peel, apple peel, pear peel, orange, tangerine, and lemon peel.

In addition, the Galactomyces peel fermentation product is Lactomyces reessii ( Galactomyces reessii ) strain, Galactomyces geotrichum ( Galactomyces geotrichum ) strain and Galactomyces candidium ( Galactomyces candidum ) 1 selected from the group consisting of strains Those prepared using strains of more than one species may be used.

The cosmetic composition according to the present invention may further include 0.1 to 10 parts by weight of a fermented Bifidobacterium seaweed product.

The cosmetic composition according to the present invention is an essence, cream, lotion, foam, nutrient lotion, cream, pack, soft water, emulsion, makeup base, external ointment, soap, liquid detergent, bath agent, sunscreen cream, sun oil, suspension, emulsion It may have one or more formulations selected from the group consisting of liquid, paste, gel, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, patch and spray.

The cosmetic composition according to the present invention contains a fermented product of Galactomyces peel containing a large amount of low molecular weight pectin by fermenting the peel using a Galactomyces spp. indicates

In addition, the cosmetic composition according to the present invention contains a fermented product of Bifidobacterium seaweed containing a large amount of low-molecular-weight carbohydrate by fermenting seaweed using a Bifidobacterium spp. It can be commercialized in various formulations such as lotions, essences, creams, and the like.

1 is a performance analysis result of a cosmetic composition prepared by a method according to an embodiment.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings so that those of ordinary skill in the art to which the present invention pertains can easily implement them. However, since the description of the present invention is merely an embodiment for structural or functional description, the scope of the present invention should not be construed as being limited by the embodiment described in the text. That is, since the embodiment may have various changes and may have various forms, it should be understood that the scope of the present invention includes equivalents capable of realizing the technical idea. In addition, since the object or effect presented in the present invention does not mean that a specific embodiment should include all of them or only such effects, it should not be understood that the scope of the present invention is limited thereby.

The meaning of the terms described in the present invention should be understood as follows.

Terms such as “first” and “second” are for distinguishing one component from another, and the scope of rights should not be limited by these terms. For example, a first component may be termed a second component, and similarly, a second component may also be termed a first component. When a component is referred to as being “connected” to another component, it may be directly connected to the other component, but it should be understood that other components may exist in between. On the other hand, when it is mentioned that a certain element is "directly connected" to another element, it should be understood that the other element does not exist in the middle. Meanwhile, other expressions describing the relationship between elements, that is, “between” and “immediately between” or “neighboring to” and “directly adjacent to”, etc., should be interpreted similarly.

The singular expression is to be understood as including the plural expression unless the context clearly dictates otherwise, and terms such as "comprises" or "have" refer to the described feature, number, step, action, component, part or these It is intended to indicate that a combination exists, and it should be understood that it does not preclude the possibility of the existence or addition of one or more other features or numbers, steps, operations, components, parts, or combinations thereof.

All terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs, unless otherwise defined. Terms defined in the dictionary should be interpreted as being consistent with the meaning of the context of the related art, and cannot be interpreted as having an ideal or excessively formal meaning unless explicitly defined in the present invention.

Hereinafter, the present invention will be described in detail.

_{The cosmetic composition according to the present invention includes water (H 2} O) and an additive including at least one selected from the group consisting of fermented Galactomyces pericarp, thickener, moisturizer, preservative, fragrance, and color.

Galactomyces peel fermented product is prepared by inoculating Galactomyces sp. strain on the peel and then fermenting it. It is possible to prepare a cosmetic composition exhibiting various functions such as prevention, moisturizing, skin wrinkle improvement, skin elasticity improvement effect, and the like.

Specifically, the galactomyces sp. strain is a protopectin lysing enzyme (protopectinase), pectinase (pectinase), polygalacturonase (polygalacturonase), pectinesterase (pectinesterase), pectinlyase (pectinlyase) during the process of fermentation of the pericarp ), etc., and can greatly lower the molecular weight by decomposing pectin.

In addition, the Galactomyces spp. strain decomposes the peel to produce useful components such as amino acids such as proline, glycine, valine, and arginine, vitamins, enzymes, and organic acids and antibacterial substances, and promotes skin absorption by lowering the molecular weight of useful components. , thereby preventing skin aging, increasing skin elasticity, and maintaining skin regeneration and moisturizing properties for a long time.

In addition, Galactomyces pericarp fermented product contains Galactomyces peptides to promote cell production, strengthen living tissues including vitamin components, and contain amino acids, enzymes, organic acids, etc. to show skin whitening function, selenium , including mineral components such as germanium, calcium, magnesium, etc., may exhibit skin wrinkle reduction performance.

The fermented Galactomyces peel as described above can be prepared using peels with a high content of pectin, and the peels may include citrus peels such as apple peels, banana peels, pear peels, oranges, tangerines, lemons, or mixtures thereof. can

Galactomyces peel fermentation product is Galactomyces reessii ( Galactomyces reessii ) strain, galactomyces geotrichum ( Galactomyces geotrichum ) strain, galactomyces candidium ( Galactomyces candidum ) Galacto containing a strain or a mixture thereof It can be prepared using a M. sp. strain, preferably, it can be prepared using a Galactomyces leechesi strain, and more preferably, a Galactomyces reessii strain (KCCM 50259) can be used. .

The Galactomyces peel fermented product may be included in a ratio of 0.1 to 10 parts by weight based on 100 parts by weight of the composition. In this case, it is difficult to expect further improvement in physical properties.

In addition, the cosmetic composition according to the present invention may further include a fermented product of Bifidobacterium seaweed, and the fermented product of Bifidobacterium seaweed is inoculated with Bifidobacterium sp. to seaweed and then fermented. By decomposing carbohydrates contained in seaweed to form low-molecular-weight carbohydrates, and increasing the introduction of various active ingredients contained in seaweeds, it is possible to enhance the skin beauty improvement performance of the cosmetic composition.

The Bifidobacterium seaweed fermented product as described above can be prepared by inoculating bifidobacterium in seaweed containing seaweed, kelp, Ecklonia cava, ragweed, mother-of-pearl, green seaweed, seaweed, agar agar, shiitake or a mixture thereof, followed by fermentation. , Preferably, the seaweed may use shiitake.

Bifidobacterium sp. strains are Bifidobacterium longum ( Bifidobacterium longum ), Bifidobacterium adole centis ( B. adolescentis ), Bifidobacterium bifidum ( B. bifidum ), Bifidobacterium breve ( B breve ), Bifidobacterium animalis ( B. animalis ), Bifidobacterium infantis ( B. infantis ) or a mixture thereof may be used. Preferably, it can be fermented using a Bifidobacterium longum strain.

The fermented Bifidobacterium seaweed may be included in an amount of 0.1 to 10 parts by weight based on 100 parts by weight of the composition. If it exceeds, it is difficult to expect further improvement in physical properties.

In addition, the cosmetic composition according to the present invention includes vitamins, peptides, high molecular weight polysaccharides, sphingolipids, fats and oils, humectants, emollients, carriers, surfactants, pigments, organic powders, UV absorbers, preservatives, bactericides, antioxidants, plant extracts , a pH adjusting agent, alcohol, a colorant, a fragrance, or an additive including a mixture thereof.

The additive may be included in a range that does not impair the physical properties of the cosmetic composition, and may be included in a ratio of 1 to 40 parts by weight.

Specifically, the vitamin may include water-soluble vitamins and fat-soluble vitamins that can be formulated in cosmetics, and the water-soluble vitamins include vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, Folic acid, vitamin C, vitamin H, etc. can be used, and vitamin salts such as thiamine hydrochloride and sodium ascorbate salt, vitamin derivatives such as ascorbic acid-2-phosphate sodium salt, and ascorbic acid-2-phosphate magnesium salt are also may include

In addition, as the fat-soluble vitamin, vitamin A, carotene, vitamin D2, vitamin D3, vitamin E, etc. can be used, and ascorbine palmitate, ascorbine stearate, ascorbine dipalmitate, dl-α tocopherol acetate, dl nicotinic acid Vitamin derivatives such as -α-tocopherol vitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, and pantothenyl ethyl ether may also be included.

As the peptide, collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin, or a mixture thereof may be used, and it may help skin elasticity and skin regeneration.

The high molecular weight polysaccharide may use xanthan gum, hydroxyethyl cellulose, sodium hyaluronate, chondroitin sulfuric acid, or a mixture thereof, or a salt derived therefrom, and improves moisture retention, emulsion stability, etc., and serves to control viscosity do

As the sphingolipid, ceramide, phytosphingosine, sphingoglycolipid or a mixture thereof may be used, and moisture retention and emulsion stability may be improved.

In addition, ester-based fats and oils, hydrocarbon-based fats and oils, silicone-based fats and oils, fluorine-based fats and oils, animal fats, vegetable fats, or mixtures thereof may be used as the fat or oil component.

Specifically, ester-based oils and fats are tri2-ethylhexanoate glyceryl, 2-ethylhexanoate cetyl, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate. , butyl stearate, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl myristate, isostearyl palmitate, octyldodecyl myristate, isocetyl isostearate, diethyl sebacate , Diisopropyl adipate, isoalkyl neopentanoate, tri (caprylic acid, capric acid) glyceryl, tri 2-ethylhexanoate trimethylol propane, triisostearate trimethylol propane, tetra2-ethyl hexanoate penta Elysitol, cetyl caprylate, decyl laurate, hexyl laurate, decyl myristate, myristyl myristate, cetyl myristate, stearyl stearate, decyl oleate, cetyl ricinoleate, laurin Isostearyl acid, isotridecyl myristate, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyldodecyl oleate, octyldodecyl linoleate, isostearate isopropyl, 2-ethylhexane San cetostearyl, 2-ethyl hexanoate stearyl, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicapric acid, di(capryl, capric acid) propylene glycol, propylene dicaprylate Glycol, neopentyl glycol dicaprate, neopentyl glycol dioctanoate, glyceryl tricaprylate, glyceryl triundecylate, glyceryl triisopalmitate, glyceryl triisostearate, octyldodecyl neopentanoate, octanoic acid Sostearyl, octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, octyldecyl isostearate, polyglycerol oleate ester, polyglycerol isostear Acid ester, triisocetyl citrate, triisoalkyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, cetyl lactate, octyldecyl lactate, triethyl citrate, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate , diisostearyl malate, 2-ethylhexyl hydroxystearate, di2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, di-sebacinate Octyl, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, phytsteryl isostearate, phytsteryl oleate, 12-stearate Isocetyl alloylhydroxystearate, 12-stealoylhydroxystearate stearyl, 12-stealoylhydroxystearate isostearyl, etc. can be used.

Hydrocarbon-based fats and oils may include squalene, squalane, liquid paraffin, α-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybuden, microcrystalline wax, petrolatum, or mixtures thereof.

Silicone-based oils and fats include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane/methylcetyloxysiloxane copolymer, dimethylsiloxane/methylstealloxysiloxane copolymer, alkyl Modified silicone oil, amino-modified silicone oil, etc. can be used.

As the fluorine-based fat, perfluoropolyether or the like can be used.

Animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, saffron oil, soybean oil, corn oil, rapeseed oil, sesame oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil, cottonseed oil, Coconut oil, Kukui nut oil, wheat germ oil, rice germ oil, shea butter, cranberry colostrum oil, marker damia nut oil, meadow home oil, egg yolk oil, tallow, horse oil, mink oil, orange raffia oil, jojoba oil, candelabra wax, carr Nava wax, liquid lanolin, hydrogenated castor oil, or a mixture thereof may be used.

Moisturizers include serine, glutamine, sorbitol, mannitol, pyrrolidone-sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol, polypropylene glycol, polyglycerin, lactic acid, lactate or Mixtures thereof may be used.

Water-soluble polymers include carboxyvinyl polymer, polyaspartate, tragacanth, xanthan gum, methylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, carboxymethylcellulose, water-soluble chitin, chitosan, dextrin or these mixtures and the like can be used.

As the fat-soluble polymer, polyvinylpyrrolidone/eicocene copolymer, polyvinylpyrrolidone/hexadecene copolymer, nitrocellulose, dextrin fatty acid ester, polymer silicone, or a mixture thereof may be used.

The emollient agent may be a long-chain acyl glutamic acid cholesteryl ester, hydroxystearic acid cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid, lanolin fatty acid cholesteryl ester, or a mixture thereof.

As the surfactant, a nonionic surfactant, an anionic surfactant, a cationic surfactant, an amphoteric surfactant, etc. can be used.

Specifically, as the nonionic surfactant, self-emulsifying glycerin monostearate, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerol fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbitan fatty acid Ester, POE glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE hydrogenated castor oil, POE castor oil, POE/POP (polyoxyethylene/polyoxypropylene) copolymer, POE/POP alkyl ether, polyether-modified silicone, Lauric acid alkanolamide, alkylamine oxide, hydrogenated soybean phospholipid, or a mixture thereof may be used.

Anionic surfactants include fatty acid soap, α-acyl sulfonate, alkyl sulfonate, alkyl allyl sulfonate, alkyl naphthalene sulfonate, alkyl sulfate, POE alkyl ether sulfate, alkyl amide sulfate, alkyl phosphate, POE alkyl phosphate, alkyl amide. Phosphate, alkyloylalkyltaurine, N-acylamino acid, POE alkylethercarboxylate, alkylsulfosuccinate, sodium alkylsulfoacetate, acylated hydrolyzed collagen peptide salt, perfluoroalkylphosphate ester, or mixtures thereof etc. can be used.

Cationic surfactants include alkyltrimethylammonium chloride, stearyltrimethylammonium chloride, stearyltrimethylammonium bromide, cetostearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylbenzylammonium chloride, behenyltrimethylammonium bromide, chloride Benzalkonium, diethylaminoethylamide stearate, dimethylaminopropylamide stearate, quaternary ammonium salts of lanolin derivatives, or mixtures thereof can be used.

The amphoteric surfactant is a carboxybetaine type, an amidebetaine type, a sulfobetaine type, a hydroxysulfobetaine type, an amidesulfobetaine type, a phosphobetaine type, an aminocarboxylate type, an imidazoline derivative type, an amidamine type, or these mixtures of can be used.

As the pigment, an organic pigment or an inorganic pigment or a mixture thereof may be used. Inorganic pigments include silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, bengala, clay, bentonite, titanium coated mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium dioxide, aluminum oxide, sulfuric acid. Calcium, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine, chromium oxide, chromium hydroxide, calamine, or mixtures thereof are representative examples, and organic pigments include polyamide, polyester, polypropylene, Polystyrene, polyurethane, vinyl resin, urea resin, phenolic resin, fluororesin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene/styrene copolymer, silk powder, cellulose, CI pigment yellow , CI Pigment Orange or a mixture thereof can be cited as a representative example.

The organic powder may be a metal soap such as calcium stearate; alkyl phosphate metal salts such as sodium zinc cetylate, zinc laurylate, and calcium laurylate; acylamino acid polyvalent metal salts such as calcium N-lauroyl-β-alanine, zinc N-lauroyl-β-alanine, and calcium N-lauroyl glycine; amidesulfonic acid polyvalent metal salts such as calcium N-lauroyl-taurine and calcium N-palmitoyl-taurine; N-acyl basic amino acids such as Nε-lauroyl-L-lysine, Nε-palmitoylizine, Nα-paritoylolnithine, Nα-lauroylarginine, and Nα-hydrogenated beef fatty acid acylarginine; N-acyl polypeptides, such as N-lauroyl glycyl glycine; α-amino fatty acids such as α-aminocaprylic acid and α-aminolauric acid; Representative examples include polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene/styrene copolymer, ethylene tetrafluoride, or mixtures thereof.

Fillers may include natural ceramic components such as illite, bentonite, hectorite, vermiculite, zeolite, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, and the like.

Ultraviolet absorbers are para-aminobenzoic acid, para-aminobenzoate ethyl, para-aminobenzoic acid amyl, para-aminobenzoate octyl, salicylate ethylene glycol, salicylate phenyl, octyl salicylate, benzyl salicylate, butyl salicylate, homomentyl salicylate, benzyl cinnamate. , Para-methoxycinnamic acid-2-ethoxyethyl, para-methoxycinnamic acid octyl, dipara-methoxycinnamic acid mono2-ethylhexaneglyceryl, para-methoxycinnamic acid isopropyl, diisopropyl diisopropyl cinnamic acid ester mixture, urocanin Acid, ethyl urokanate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and salts thereof, dihydroxymethoxybenzophenone, sodium dihydroxymethoxybenzophenonedisulfonate, dihydroxybenzophenone, tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino-p-(carbo-2'-ethylhexyl-1'-oxy)-1, Representative examples include 3,5-triazine, 2-(2-hydroxy-5-methylphenyl)benzotriazole, or mixtures thereof.

Disinfectants include hinokitiol, triclosan, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, zinc phyllithione, benzalkonium chloride, photosensitizer Sodium 301, mononitroguaial sodium, undecyrenic acid, or mixtures thereof are representative examples.

Examples of the antioxidant include butylhydroxyanisole, propyl gallic acid, and ellisorbic acid. Representative examples of the pH adjusting agent include citric acid, sodium citrate, malic acid, sodium malate, fmalic acid, sodium fmarate, succinic acid, sodium succinate, sodium hydroxide, sodium monohydrogen phosphate, or mixtures thereof.

Representative examples of the alcohol include ethanol, cetyl alcohol, or a mixture thereof.

In addition, the mixing|blending component which may be added other than this is not limited to this, Any of the said components can be mix|blended within the range which does not impair the objective and effect of this invention.

And, according to a preferred embodiment of the present invention, the cosmetic composition may include water (H ₂ O) as a solvent, and water may serve as a solvent to uniformly mix each component, purified water, distilled water , sterile water, deionized water, or a mixture thereof may be used.

The cosmetic composition according to the present invention includes lotion, essence, cream, lotion, foam, nutrient lotion, cream, pack, flexible lotion, emulsion, makeup base, external ointment, soap, liquid detergent, bath agent, sunscreen cream, sun oil, Suspensions, emulsions, pastes, gels, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations, patches and sprays, etc. may be prepared, but are not limited thereto.

As described above, the cosmetic composition according to the present invention includes a fermented Galactomyces peel containing a large amount of low molecular weight pectin by fermenting the peel using a Galactomyces spp. It exhibits various functions such as prevention.

In addition, the cosmetic composition according to the present invention contains a fermented product of Bifidobacterium seaweed containing a large amount of low-molecular-weight carbohydrate by fermenting seaweed using a Bifidobacterium spp. It can be commercialized in various formulations such as lotions, essences, creams, and the like.

Hereinafter, the present invention will be described in more detail by way of examples.

The presented examples are only specific examples of the present invention, and are not intended to limit the scope of the present invention.

<Preparation Example 1> Preparation of Galactomyces peel fermented product

(1) microbial culture

Galactomyces reessii strain (Galactomyces reessii KCCM 50259), Galactomyces geotrichum SFC20140101 strain, Galactomyces candidium ( Galactomyces candidum SFC20141123) strain on the banana peel, respectively, inoculated and then profectinase ( A strain producing a high content of protopectinase) and pectinase was isolated. As for the strains, those received from ATCC and the Marine Life Resources Depositary and Preservation Agency were used, respectively.

Galactomyces reessii KCCM 50259), Galactomyces geotrichum SFC20140101), and Galactomyces candidium (Galactomyces candidum SFC20141123) strains were inoculated in the culture medium to which the banana peel decomposition was added. Then, a strain producing a high content of protoectinase and pectinase was isolated by stirring and culturing at 20 to 23° C. for 48 hours.

The culture medium was based on 100 parts by weight of the culture medium, including 5 parts by weight of banana peel decomposition product, 0.5 parts by weight of glucose, 0.6 parts by weight of peptone, and 0.6 parts by weight of an enzyme extract and the rest of distilled water, and a pH of 5.0 was used. , each strain was inoculated at a concentration of ^{1 × 10 5 / mL.}

(2) analysis of degradation activity

For isolation and identification of strains producing high content of profectinase and pectinase from banana peel, take 1 mL of the strain culture solution, mix 0.25 mL of 0.125% carbazole, add 3 mL of sulfuric acid, and bath) for 15 minutes, absorbance was measured at 525 nm, and the content was calculated using β-D-galacturonic acid obtained from the calibration curve as a standard material.

As a result, it was confirmed that the galacturonic acid concentration of the culture medium of the Galactomyces reessii KCCM 50259 was 2.51%, and in the case of the Galactomyces geotrichum SFC20140101 culture medium, 1.03%, galacturonic acid In the case of the M. candidium strain ( Galactomyces candidum SFC20141123) culture, it was found to be 1.13%, and it was confirmed that the Galactomyces reessii KCCM 50259 had the best pectin degradation performance. In addition, as a result of measuring the content of pectin using the hot water extract (95 ° C.) of the decomposed banana peel as a control, it was confirmed that the content of galacturonic acid was 0.31%, and the content of low molecular weight pectin was greatly increased through fermentation of the strain. I was able to confirm that it was possible.

(3) Preparation of fermented Galactomyces peel

The identified strain was cultured in a medium of pH 5.0 containing 0.5% glucose, 0.6% peptone and 0.2% yeast extract at 30 °C for 20 hours in a stirred culture machine to prepare a seed strain.

Each strain was added to a culture solution of pH 5.0 containing 10 parts by weight of banana peel decomposition, 1 part by weight of glucose, 1 part by weight of peptone, and 2 parts by weight and 86 parts by weight of an enzyme extract 1 × 10 ⁵ pieces/mL 2 mL of the culture medium was inoculated, and cultured at 22 ° C. for 96 hours to prepare a culture, sterilized by heating, and filtered through a 200 mesh sieve to obtain a fermented Galactomyces pericarp.

The prepared fermented Galactomyces peel was concentrated under reduced pressure to prepare a powder of the fermented Galactomyces peel.

<Production Example 2>

(1) microbial culture

Bifidobacterium longum strain (KACC 91563) was received, inoculated into MRS medium, and cultured at 37° C. for 72 hours.

Trypticase-Proteose peptone-Yeast extract (TPY) 3 parts by weight, powdered skim milk 2 parts by weight, potassium dihydrogen phosphate (KH ₂ PO ₄ ) 0.2 parts by weight, L-cysteine 0.5 parts by weight, sodium propionate 0.5 parts by weight, ground horseradish 10 parts by weight and 83.8 parts by weight of distilled water were mixed, and fermented at 37° C. for 120 hours to prepare a fermented product.

The prepared fermented product was sterilized by heating, and filtered through a 200 mesh sieve to obtain a fermented product of Bifidobacterium seaweed, which was concentrated under reduced pressure to prepare a fermented product of Bifidobacterium seaweed.

As a result of analyzing the carbohydrate content of the prepared Bifidobacterium seaweed fermented product, it was confirmed that the carbohydrate content was 43.5% in the case of the Bifidobacterium seaweed fermented product, whereas the carbohydrate content was 15.3% in the control group, ground chives. It was confirmed that the carbohydrate content was significantly increased through Bifidobacterium fermentation.

<Example 1>

A lotion type cosmetic composition was prepared by blending each component with the composition shown in Table 1 below.

A lotion-type cosmetic composition is prepared by adding glycerin, butylene glycol, propylene glycol and a preservative to purified water and dissolving it through stirring, mixing polyoxyethylene hydrogenated castor oil with the mixture, and then heating to 60 ℃ to dissolve it. , a fragrance was added and dissolved, and then the mixed fermented extract, ethanol, triethanolamine, and a pigment were added, stirred sufficiently, and then aged to prepare a cosmetic composition according to Example 1.

<Example 2>

A lotion type cosmetic composition was prepared by blending each component with the composition shown in Table 2 below.

The lotion type cosmetic composition is prepared by heating a mixture of propylene glycol, carboxypolymer, preservative, and purified water to a temperature of 80 to 85 ° C. Late, lipophilic glycerin monostearate. Stearic acid, glyceryl stearate/PEG-400 stearate, and triethanolamine were respectively added and dissolved by heating at a temperature of 80 to 85° C., followed by emulsification using an emulsifier to prepare an emulsified mixture. The emulsified mixture was cooled to a temperature of 50 °C while stirring using a stirrer, a fragrance was added, and then cooled to a temperature of 45 °C, a dye was added and then cooled to a temperature of 35 °C. A lotion-type cosmetic composition was prepared by adding the mixed extract to the cooling water, cooling it to a temperature of 25° C., and then aging it.

<Example 3>

A cream-type cosmetic composition was prepared by mixing each component in the composition shown in Table 3 below.

The cream-type cosmetic composition is prepared by mixing and stirring carboxyvinyl polymer, butylene glycol, glycerin and purified water and then heating to a temperature of 80 to 85 ° C. Stearic acid, cetyl alcohol, glyceryl monostearate, polyoxyethylene sorbitan monostearate, sorbitan sesquioleate, glyceryl stearate/polyoxyethylene stearate, wax, liquid paraffin, squalane, caprylic/capric Triglyceride was added and dissolved by heating at a temperature of 80 to 85° C. After dissolution, triethanolamine was added and emulsified to prepare an emulsion. The prepared emulsion was cooled to a temperature of 35 °C while stirring using a stirrer, and then the mixed extract was added and cooled to a temperature of 25 °C.

<Example 4>

Essence-type cosmetic compositions were prepared by blending each component in the composition shown in Table 4 below.

Essence-type cosmetic composition is prepared by mixing glycerin, butylene glycol, propylene glycol, carboxyvinyl polymer and xanthan gum, stirring and dissolving, to prepare a mixture, mixing polyoxyethylene hydrogenated castor oil with the mixture, and then heating to a temperature of 50 ° C. was dissolved, flavorings and purified water were respectively added to dissolve, mixed fermented extract, triethanolamine, and preservatives were added, stirred and then aged.

<Example 5>

A cosmetic composition was prepared in the same manner as in Example 1, except for mixing 1 part by weight of fermented Bifidobacterium seaweed powder instead of 1 part by weight of purified water.

<Example 6>

A cosmetic composition was prepared in the same manner as in Example 2, except that 1 part by weight of fermented Bifidobacterium seaweed powder was mixed instead of 1 part by weight of purified water.

<Example 7>

A cosmetic composition was prepared in the same manner as in Example 3, except that 1 part by weight of fermented Bifidobacterium seaweed powder was mixed instead of 1 part by weight of purified water.

<Example 8>

A cosmetic composition was prepared in the same manner as in Example 4, except that 1 part by weight of fermented Bifidobacterium seaweed powder was mixed instead of 1 part by weight of purified water.

<Experimental Example> Performance evaluation of cosmetic compositions

(1) Cytotoxicity evaluation

The cytotoxicity of Galactomyces pericarp fermented products and Bifidobacterium seaweed fermented products prepared by the methods according to Preparation Examples 1 and 2 were evaluated, and the results are shown in Table 5 below. Cytotoxicity was evaluated by setting as Control 1 and Control 2.

^{For cytotoxicity evaluation, 1 × 10 5} fibroblasts were placed in a 24-well test plate and cultured for 24 hours, then PBS (Phosphate buffered saline) was injected into each well and washed, and 10% of each well was added. 1 mL of DMEM medium containing fetal bovine serum and 100 μl of the Galactomyces peel ferment and Bifidobacterium seaweed fermented in Preparation Examples 1 and 2 were respectively injected and cultured for 24 hours. Galactomyces peel fermented product and Bifidobacterium seaweed fermented product were each prepared by mixing 10 μg with distilled water to make 100 μl. After 24 hours had elapsed, the medium was removed, and 500 μl of cell culture medium and 60 μl of MTT solution (2.5 mg/mL) were injected, and then cultured in a _{37° C. CO 2 incubator for 3 hours.} After removing the medium, 500 μl of isopropanol-HCl mixed solution (0.04N) was injected, respectively, and the cells were lysed by shaking incubation for 5 minutes, and the absorbance of the supernatant obtained by obtaining the supernatant was measured at 565 nm. The cell viability (%) was measured using Equation 1 below, and the concentration of the sample that did not affect the cell survival was determined.

[Equation 1]

Cell viability (%) = [(S _t -B _o )/(B _t /B _o )] × 100

(However, in Equation 1, B _o is the absorbance of the sample in which only the cell culture medium is developed, B _t is the absorbance of the control sample not supplied with the fermented product, and S _t is the absorbance of the sample supplied with the fermented product will)

As shown in Table 1, it was confirmed that the prepared fermented Galactomyces pericarp and fermented Bifidobacterium seaweed did not exhibit cytotoxicity, thereby confirming safety.

(2) Evaluation of anti-aging performance by ultraviolet rays

The anti-aging performance of the prepared cosmetic composition was evaluated, and the results are shown in Table 5 and FIG. 1 .

To evaluate the anti-aging performance, 1 × 10 ⁵ keratinocytes were placed in a 24-well test plate and adhered for 24 hours. Each well was washed twice with PBS and 500 μl of PBS was supplied to the wells. Each well is supplied to the chamber, and UV B is irradiated with ^{an intensity of 10 mJ/cm 2} for 5 minutes, then PBS is removed, 1 ml of DMEM medium containing 10% FBS and Galactomyces peel ferment, B.P. 5 μl, 10 μl, and 100 μl of the G. seaweed fermented product were respectively injected, and 10 μl, 20 μl, and 200 μl of a mixture of Galactomyces pericarp ferment and Bifidobacterium seaweed ferment were injected, respectively, for 24 hours. cultured. After 24 hours, the medium was removed, and 500 μl of cell culture medium and 60 μl of MTT solution (2.5 mg/mL) were supplied to each well, and then cultured in a _{37° C. CO 2 incubator for 2 hours.} The medium was removed, 500 μl of isopropanol-HCl (0.04 N) was added, and the cells were lysed by shaking and stirring for 5 minutes to obtain 100 μl of the supernatant, and then absorbance at 565 nm was measured. (%) was evaluated.

As a result of evaluating the cell viability of the untreated control group irradiated with UV light, it was confirmed that the cell viability was 53.6%, and in the case of Control 1 to which 0.5% of pectin was added and Control 2 to which Hichigan extract was added, cell viability decreased or the untreated control On the other hand, it was confirmed that the cell viability was significantly increased when Preparation Example 1 was added, on the other hand, in the case of Preparation Example 3, it was confirmed that the cell viability was significantly increased. Accordingly, it was confirmed that the mixture of fermented Galactomyces peel, fermented Galactomyces peel, and fermented Bifidobacterium seaweed had an effect of preventing skin damage caused by UV rays.

(3) Wrinkle improvement efficacy evaluation 1

Cosmetic compositions having the formulations of Examples 2, 3, 6 and 7 below were administered to 30 adult women aged 30 to 60 years with fine lines and coarse wrinkles, twice a day for 8 weeks, respectively, around the eyes on both sides of the face for 8 weeks. was applied to evaluate the wrinkle improvement effect of the cosmetic composition, and the results are shown in Table 6 below.

Wrinkle improvement efficacy was evaluated by using a skin wrinkle meter to evaluate changes in skin wrinkles before and after application of the cosmetic composition, and was performed by measuring the distance between the highest height and the lowest valley among the wrinkle curves. In addition, the case of applying the formulation to which pectin was added in the same ratio and the formulation added to the extract of Japanese hibiscus in the same ratio was set as Control 1 and Control 2, respectively.

As a result of evaluating the wrinkle improvement performance of the cosmetic compositions according to Examples, as shown in Table 2, when the cosmetic compositions of Examples 2 and 3 were applied, it was confirmed that the wrinkle improvement effect was very large, and compared with Control 1, pectin was It was confirmed that the wrinkle improvement performance was further improved due to low molecular weight. In particular, in the case of Examples 6 and 7, it could be confirmed that the wrinkle improvement performance was further improved due to the addition of the fermented Bifidobacterium chives.

(4) Wrinkle improvement efficacy evaluation 2

The cosmetic compositions having the formulations of Examples 4 and 8 below for 20 adult women aged 30 to 60 years with fine lines and coarse wrinkles were applied to the facial area twice a day for 8 weeks, respectively, for 4 weeks. The wrinkle improvement effect was evaluated, and the results are shown in Table 7 below. In addition, the case of applying the formulation to which pectin was added in the same ratio and the formulation added to the extract of Japanese hibiscus in the same ratio was set as Control 1 and Control 2, respectively.

Wrinkle improvement efficacy was evaluated through image analysis of wrinkles. Before applying the cosmetic composition, a replica was collected around the mouth (Xantopren, Bayer), and after applying the cosmetic composition, the replica was applied in the same area around the mouth. The density of wrinkles was measured by two-dimensional analysis of wrinkles through image analysis. The measurement results of the wrinkle density by image analysis are shown in Table 7 by averaging the reduction rate for the wrinkle density before use.

As shown in Table 7, as a result of evaluating the wrinkle-improving efficacy, it was confirmed that when the cosmetic composition according to Example 5 was applied, the wrinkle density was significantly reduced, thereby exhibiting excellent wrinkle-improving efficacy, and Galactomyces pericarp fermented product It was confirmed that even in the case of inclusion of wrinkle, it was confirmed that it had a significant effect on wrinkle improvement, and it was determined that skin elasticity could also be increased, as can be confirmed through the reduction rate of wrinkle density.

(5) Evaluation of moisturizing efficacy

The cosmetic compositions of Examples 4 and 8 were uniformly applied to the back of the hand to 40 adult women aged 30 to 60 years, and the skin moisture content was measured 5 times at 20-minute intervals, and the results are shown in the table below. 8 is shown.

The skin moisture content was repeatedly measured using a skin moisture meter (model name: IC-HSP001), and was carried out under conditions in which a relative humidity of 40 to 45% and a temperature of 23 to 24 ° C were maintained, and the average value of the moisture content was measured.

Referring to Table 8, when the cosmetic compositions of Examples 1 and 5 were applied, it was confirmed that the skin moisture content was significantly increased compared to before application, thereby showing a moisturizing effect.

In particular, in the case of Example 7, it was confirmed that the moisturizing effect was the most excellent, and it was confirmed that the skin improvement effect was further improved.

Through the above results, the cosmetic composition according to the present invention contains a fermented Galactomyces peel containing a large amount of low molecular weight pectin by fermenting the peel using a Galactomyces spp. , it was confirmed that it exhibits various functions such as anti-aging.

In addition, the cosmetic composition according to the present invention contains a fermented product of Bifidobacterium seaweed containing a large amount of low-molecular-weight carbohydrate by fermenting seaweed using a Bifidobacterium spp. It was confirmed that it can be commercialized in various formulations such as lotions, essences, and creams.

The detailed description of the preferred embodiments of the present invention disclosed as described above is provided to enable any person skilled in the art to make and practice the present invention. Although the above has been described with reference to preferred embodiments of the present invention, it will be understood by those skilled in the art that various modifications and changes can be made to the present invention without departing from the scope of the present invention. For example, a person skilled in the art may use each configuration described in the above-described embodiments in a way that is combined with each other. Accordingly, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

The present invention may be embodied in other specific forms without departing from the spirit and essential characteristics of the present invention. Accordingly, the above detailed description should not be construed as restrictive in all respects but as exemplary. The scope of the present invention should be determined by a reasonable interpretation of the appended claims, and all modifications within the equivalent scope of the present invention are included in the scope of the present invention. The present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein. In addition, claims that are not explicitly cited in the claims may be combined to form an embodiment, or may be included as new claims by amendment after filing.

Claims (5)

0.1 to 10 parts by weight of fermented Galactomyces pericarp;
Vitamins, peptides, polysaccharides, sphingolipids, oils and fats, humectants, emollients, surfactants, pigments, organic powders, fillers, UV absorbers, preservatives, fungicides, antioxidants, plant extracts, pH regulators, alcohols, pigments and fragrances 1 to 40 parts by weight of an additive comprising at least one selected from the group consisting of; and
Water (H ₂ O) 50 to 98.9 parts by weight; Cosmetic composition comprising a. According to claim 1,
The fermented Galactomyces peel is a cosmetic composition, characterized in that it is prepared by fermenting one or more peels selected from the group consisting of banana peel, apple peel, pear peel, orange, tangerine and lemon peel. According to claim 1,
The galactomyces peel fermentation product is Lactomyces reessii ( Galactomyces reessii ) strain, galactomyces geotrichum ( Galactomyces geotrichum ) strain and Galactomyces candidium ( Galactomyces candidum ) At least one selected from the group consisting of strains A cosmetic composition, characterized in that it is prepared using a strain. According to claim 1,
A cosmetic composition, characterized in that it further comprises 0.1 to 10 parts by weight of a fermented product of Bifidobacterium seaweed. According to claim 1,
Essence, cream, lotion, foam, nourishing lotion, cream, pack, soft water, emulsion, makeup base, external ointment, soap, liquid detergent, bath agent, sunscreen cream, sun oil, suspension, emulsion, paste, gel, powder , soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, cosmetic composition, characterized in that it has one or more formulations selected from the group consisting of patches and sprays.

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