CN111601584A

CN111601584A - Antibacterial cosmetic composition comprising Lactobacillus plantarum culture

Info

Publication number: CN111601584A
Application number: CN201880066809.0A
Authority: CN
Inventors: 白世熙; 徐溶基; 辛民景
Original assignee: CJ CheilJedang Corp
Current assignee: CJ CheilJedang Corp
Priority date: 2017-11-16
Filing date: 2018-11-16
Publication date: 2020-08-28
Also published as: TWI686209B; KR20190056177A; TW201922224A; WO2019098752A1; KR102032546B1

Abstract

The present application relates to antibacterial cosmetic compositions for skin comprising lactobacillus plantarum cultures.

Description

Antibacterial cosmetic composition comprising Lactobacillus plantarum culture

Technical Field

The present application relates to a cosmetic composition having antibacterial effect comprising a single lactic acid culture, and more particularly, to an antibacterial cosmetic composition for skin comprising a lactobacillus plantarum CJLP133 culture.

Technical Field

The human skin protects the human body from various oxidants, air pollution, heavy metals, ultraviolet radiation (UV), etc., prevents bacteria, fungi, viruses, etc. from invading the skin, and has the function of preventing water loss as a protective barrier. The structure of the skin is roughly divided into three layers of epidermis, dermis and subcutaneous fat, and the dermis layer, which accounts for 90% of the skin, is composed of collagen, elastin, hyaluronic acid and glycoprotein. Collagen is the major component of skin, an important fibrin protein, which accounts for about 25% of the total weight of human protein. It constitutes most connective tissues and has important roles such as intercellular matrix function, structural support function, induction of cell division and differentiation, provision of skin tensile strength, and the like.

In addition, it is known that fatty acids, higher alcohols, proteins, etc. present in sebum and sweat components released from the body and cosmetic components may be decomposed into toxic substances by skin flora present on the skin, thereby causing skin inflammation, and further, secondary infection caused by exposure to pathogenic microorganisms may induce various skin diseases (e.g., acne, itching, dermatitis, allergy, etc.) due to damage of the skin barrier by ultraviolet rays from sunlight.

Therefore, it is very important in cosmetics to maximize the effects of each ingredient and minimize the side effects thereof to prevent skin aging. In this regard, there is a need to develop a cost-effective material that inhibits the growth of harmful bacteria without side effects, and an easy-to-use cosmetic containing the same.

Lactic acid bacteria are bacteria that have a beneficial effect in the human body, producing lactic acid through the fermentation of carbohydrates or fibers. To date, about 400 species of lactic acid bacteria have been found, and about 18 species have been developed as commercial products.

Once lactic acid bacteria enter the human intestinal tract, they secrete specific substances through their own metabolic activities. Since these substances have beneficial effects on the human body, many studies have been made on lactic acid bacteria. The effects of lactic acid bacteria on antibacterial, antioxidant and immunological activities have been reported. In recent years, research has been focused on whitening and moisturizing effects of lactic acid bacteria, and the possibility as a cosmetic material has been increasing. Among lactic acid bacteria, many studies have been made on the action of lactobacillus, and enhancement of immunity, antibacterial action, skin whitening, improvement of atopic dermatitis, and the like have been reported.

Among them, the plant-derived lactic acid bacteria (POLAB) are lactic acid bacteria isolated from fermented foods of plant materials (e.g., pickles, kimchi, etc.), and are different from dairy-derived lactic acid bacteria.

The lactic acid bacteria of plant origin not only include species at least 10 times more than animal lactic acid bacteria, but also have excellent adaptability to the external environment. In particular, the plant lactic acid bacteria isolated from kimchi having high salt content, low pH and high content of natural antibacterial substances can grow in extreme environments, and thus the plant-derived lactic acid bacteria have excellent productivity of various physiologically active substances.

Representative physiologically active substances may include antibacterial peptides, immunostimulants, gamma-aminobutyric acid (GABA), which exhibit various physiological functions including anti-inflammatory and immunosuppressive effects, and the like. In addition, lactic acid bacteria are heat stable peptides and produce bacteriocins as antimicrobial agents. Bacteriocins are natural antimicrobial proteins or proteinaceous substances produced by a variety of microorganisms, generally referring to substances having bactericidal mechanisms against bacterial strains that are morphologically and phylogenetically similar to the bacteriocin-producing microorganisms. Therefore, the development of cosmetic materials using lactic acid bacteria cultures or lactic acid bacteria fermented plant extracts and the like containing these substances is actively underway, and this progress is in line with the latest trend of being favorable for environmental protection and safety materials.

Nowadays, consumers expect not only general care effects in cosmetics but also various functions thereof (e.g., anti-aging, fine wrinkle improvement, anti-irritation, anti-inflammation, whitening effects, etc.).

In recent years, various plant extracts have been used for the purpose of these cosmetics, and there is an increasing desire for products based on a new concept that shows an improved range of performance characteristics by combining various characteristics.

Documents of the prior art

Patent document

(patent document 1) KR 10-1486999B 1(2015.01.21)

Disclosure of Invention

Technical problem

In line with the environmental era, the inventors of the present application have made efforts to research the cosmetic effects of the kimchi-derived lactic acid bacteria culture, which is safe to the skin, does not have any side effects and can be effectively used for antibacterial skin care through antibacterial activity, and in addition, the possibility of its development in domestic industrialization. As a result, the inventors confirmed that the lactobacillus plantarum CJLP133 culture has antibacterial activity for skin, thereby completing the present application.

Technical scheme

It is an object of the present application to provide an antibacterial cosmetic composition for skin comprising a culture of lactobacillus plantarum CJLP133 in which the bacterial bodies of lactobacillus plantarum CJLP133 deposited under accession number KCTC 11403BP were removed.

The invention has the advantages of

The present application provides a method for preparing a lactobacillus plantarum CJLP133 culture, which is safe to the skin without side effects, using lactobacillus plantarum strains (i.e., skin-beneficial bacteria), and also provides a cosmetic composition having antibacterial activity comprising the lactobacillus plantarum CJLP133 culture.

Drawings

FIG. 1 is a graph illustrating the experimental results of antibacterial activity of Lactobacillus plantarum CJLP133 culture; 2 a: staphylococcus aureus, 2 b: staphylococcus epidermidis, 2 c: escherichia coli O157: h7, 2 d: pseudomonas aeruginosa, 2 e: propionibacterium acnes.

Detailed Description

In order to accomplish the above objects, an aspect of the present application provides an antibacterial cosmetic composition for skin comprising a culture of lactobacillus plantarum CJLP133, which comprises a bacterium body from which lactobacillus plantarum CJLP133 deposited under accession number KCTC 11403BP was removed.

Hereinafter, the antibacterial cosmetic composition for skin comprising lactobacillus plantarum culture of the present application is explained in detail.

As used herein, the term "lactobacillus plantarum" refers to a microorganism of the genus lactobacillus, which is a gram-negative bacterium capable of producing lactic acid. It is found in fermented foods such as sauerkraut, pickles, sauerkraut, etc.

The lactobacillus plantarum strain may specifically be lactobacillus plantarum CJLP133 strain, and it may be the strain deposited under accession number KCTC 11403 BP.

Specifically, lactobacillus plantarum CJLP133 strain was deposited at international depository organization korean typical culture collection (KCTC) of the institute of bioscience and biotechnology (KRIBB) under the budapest treaty on 16/10 2008, and assigned registration number KCTC 11403 BP.

As used herein, the term "culture" refers to a medium comprising a byproduct produced by culturing a strain of a microorganism in the medium, wherein the strain is allowed to take up and metabolize nutrients contained in the medium. The term "culture" includes all preparations which can be formed using a culture medium (e.g., a diluent or a concentrate of the culture medium, a dried product obtained by drying the culture medium, a crude or purified product of the culture medium, a mixture thereof, and the like).

Specifically, the culture may be a culture obtained by culturing lactobacillus plantarum CJLP133 strain, or may be a culture obtained by culturing a strain from which bacterial bodies are removed after the culture. That is, a culture medium from which the bacterial body has been removed can be used as the culture.

More specifically, the culture may be obtained by a method comprising: (a) culturing Lactobacillus plantarum CJLP133 strain through an inoculation medium; (b) centrifuging the resulting product of step (a); and (c) recovering the supernatant from the resulting product of step (b).

In step (a), the medium may be any medium used in the art as long as the medium can culture the strain and recover the culture after the culture, specifically a liquid medium, and more specifically an MRS liquid medium.

In order to obtain pure isolates, step (a) may further comprise the step of plating the lactobacillus plantarum strain on a solid medium and culturing and collecting those colonies which are confirmed to be pure isolates, prior to step (a).

In step (b), centrifugation can be performed using a high speed centrifuge at 1,500rpm to 3,500rpm, specifically 2,000rpm to 3,000rpm, and more specifically 2200rpm to 2800rpm for 10 to 30 minutes, specifically 15 to 25 minutes, and more specifically 17 to 23 minutes at 1 ℃ to 7 ℃, specifically 2 ℃ to 6 ℃, and more specifically 3 ℃ to 5 ℃.

Step (c) may further comprise the step of filtering the supernatant. In addition, the step (c) may further comprise the step of pulverizing the filtered supernatant using a lyophilizer.

As used herein, the term "antibacterial" refers to the action of inhibiting the growth of microorganisms (e.g., dermatologically-associated bacteria).

In the present application, the culture may be directed against a microorganism selected from the group consisting of staphylococcus aureus, staphylococcus epidermidis, escherichia coli O157: at least one of H7, Pseudomonas aeruginosa and Propionibacterium acnes has antibacterial activity, but the bacteria are not limited thereto.

Further, the composition of the present application may be a composition that can improve skin diseases induced by microorganisms by antibacterial activity.

As used herein, the term "ameliorating" refers to suppressing or inhibiting the symptoms of a dermatological condition caused by bacteria, or alleviating the symptoms of a dermatological condition that has occurred on the skin, using the compositions of the present application.

In the present application, the cosmetic composition may be formulated in the form of a solution, a topical ointment, cream, foam, nutritional emollient, soft emollient, filler (pack), soft water, milky lotion, makeup base, essence, soap, liquid cleanser, bath agent, sunscreen, suntan oil, suspension, emulsion, paste, gel, lotion, powder, soap, surfactant-containing cleanser, oil, foundation, emulsion foundation, wax foundation, patch, and spray, but the formulation is not limited thereto.

In the present application, the cosmetic composition may further comprise at least one cosmetically acceptable carrier for incorporation into the cosmetics of ordinary skin. As conventional components of the cosmetically acceptable carrier, for example, oil, water, surfactant, humectant, lower alcohol, thickener, chelating agent, pigment, preservative, perfume, etc. may be appropriately mixed, but the components are not limited thereto.

The cosmetically acceptable carrier incorporated into the cosmetic composition of the present application may vary depending on the formulation of the cosmetic composition.

When the formulation of the present application is an ointment, a paste, an emulsifiable concentrate or a gel, animal oil, vegetable oil, wax, paraffin, starch, tragacanth, a cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, or the like can be used as a carrier component, but the carrier component is not limited thereto. These carrier components may be used alone or in combination of two or more.

When the formulation of the present application is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, etc. may be used as a carrier component, and particularly, in the case of a spray, the formulation may further include a propellant such as chlorofluorocarbon, propane/butane and dimethyl ether, but the carrier component is not limited thereto. These carrier components may be used alone or in combination of two or more.

When the formulation of the present application is a solution or an emulsion, a solvent, a solubilizing agent or an emulsifier (e.g., water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1, 3-butylene glycol, etc.) may be used as a carrier component, and in particular, cottonseed oil, peanut oil, corn seed oil, olive oil, castor oil and sesame oil, aliphatic esters of glycerin, and fatty acid esters of polyethylene glycol or sorbitan may be used, but the carrier component is not limited thereto. These carrier components may be used alone or in combination of two or more.

When the formulation of the present application is a suspension, a liquid diluent (e.g., water, ethanol or propylene glycol), a suspending agent (e.g., ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester), microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, tragacanth, and the like may be used as a carrier component, but the carrier component is not limited thereto. These carrier components may be used alone or in combination of two or more.

When the formulation of the present application is a soap, an alkali metal salt of a fatty acid salt, a half ester salt of a fatty acid, a fatty acid protein hydrolysate, isethionate, a lanolin derivative, a fatty alcohol, a vegetable oil, glycerin, a sugar, or the like may be used as the carrier component, but the carrier component is not limited thereto. These carrier components may be used alone or in combination of two or more.

Another aspect of the present application provides an antibacterial method for skin comprising administering to a subject a lactobacillus plantarum culture with bacterial bodies removed.

The antimicrobial method for skin may be a method comprising administering to a subject in need of antimicrobial skin a cosmetic composition comprising a lactobacillus plantarum culture and additionally a cosmetically acceptable carrier. The cosmetically acceptable carrier is the same as described above.

The subject may or may not include a human. Specifically, the subject may be a mammal including a cow, a pig, a sheep, a chicken, a dog, a human, and the like; birds; and the like, and may include, but is not limited to, any subject having antibacterial activity by administration of the above-described composition.

Still another aspect of the present application provides the use of a composition for the preparation of a cosmetic with antibacterial activity comprising a culture of lactobacillus plantarum, from which bacterial bodies of lactobacillus plantarum have been removed.

Examples

Hereinafter, the present application will be described in detail by way of exemplary embodiments. However, it will be apparent to those skilled in the art to which the present application pertains that these exemplary embodiments are for illustrative purposes only, and are not intended to limit the scope of the present application.

Example 1 preparation of Lactobacillus plantarum CJLP133 culture

Lactobacillus plantarum CJ deposited under accession number KCTC 11403BPThe LP133 strain was plated on solid MRS medium (BD Difco, Lactobacillus MRS agar) and cultured at 37 ℃ for about 18 hours after confirmation, pure isolated colonies were collected and inoculated using a platinum loop into liquid MRS medium (BD Difco, Lactobacillus MRS broth) and cultured at 37 ℃ for about 18 hours after culture was complete with 1.0 × 10⁹CFU/mL concentration. To separate the bacterial bodies from the culture, the culture was centrifuged for 20 minutes at 4 ℃ and 2500rpm using a high speed centrifuge (labogen 1248R). After the culture supernatant was filtered, the filtered supernatant was pulverized using a lyophilizer (HETO PL 90000). The culture thus prepared was called "Lactobacillus plantarum CJLP133 culture".

Example 2 confirmation of antibacterial Activity of Lactobacillus plantarum CJLP133 culture

To confirm the antibacterial activity of the lactobacillus plantarum CJLP133 culture prepared in example 1 against skin pathogens, the transparent area for skin pathogens was measured by paper sheet (disc) diffusion assay.

A total of five strains of skin pathogens were used, and staphylococcus aureus, staphylococcus epidermidis, escherichia coli O157, which are frequently found in skin, were used: h7, Pseudomonas aeruginosa and Propionibacterium acnes. All five strains of the skin pathogen were obtained from the korean typical culture collection (KCTC) of the korean institute for bioscience and biotechnology (KRIBB). Table 1 shows detailed information of registration number, medium, culture temperature and incubation time of each strain.

Propionibacterium acnes was sealed in an anaerobic incubator using a gas packaging system (MITSUBISHI GAS CHEMICAL, AnaeroPack) and cultured anaerobically.

TABLE 1

Bacterial strains	Registration number	Culture medium	Temperature of cultivation	Incubation time
					Staphylococcus aureus	ATCC 25923	TSA	37℃	24 to 36 hours
Staphylococcus epidermidis	KCTC 1917	TSA	37℃	24 to 36 hours
					Escherichia coli O157: h7	ATCC 25922	TSA	37℃	24 to 36 hours
Pseudomonas aeruginosa	KCTC 1636	TSA	37℃	24 to 36 hours
					Propionibacterium acnes	KCTC 3314	RCM	37℃	3 to 5 days

As a control group, the material used was prepared by pulverizing liquid MRS medium via freeze-drying, and then adding sterile distilled water to a concentration of 0.5 g/mL. As a test group, materials used were prepared by adding sterile distilled water to the Lactobacillus plantarum CJLP133 culture to concentrations of 0.1g/mL, 0.5g/mL, and 2.5 g/mL.

Each skin pathogen strain was designated 1.0 × 10⁶CFU/mL concentrations were plated on media (TSA; BDDifco, tryptic Soy agar, RCM; and BD Difco enhanced clostridial media). After placing sterile paper sheets (ADVANTEC, 8mm) on the plates plated with each strain, the test material and the control material were added in an amount of 50. mu.L, respectively. After each strain was cultured according to its respective culture conditions (table 1), the diameter of the transparent region around the paper sheet (i.e., the apoptotic region of the indicator strain) was measured. A larger transparent area indicates a larger antibacterial activity, and a transparent area is not formed without the antibacterial activity.

As a result of the experiment, the control group did not form a transparent area, and the test group using the lactobacillus plantarum CJLP133 culture formed a transparent area in all five strains, thereby confirming that the lactobacillus plantarum CJLP133 culture has antibacterial activity against skin pathogens. In addition, as the concentration of the lactobacillus plantarum CJLP133 culture increased, the size of the transparent area increased, confirming that the antibacterial activity increased in a concentration-dependent manner. The results of the experiment are shown in table 2 and fig. 1.

TABLE 2

(no inhibition zone, + less than 10.0mm, + 10.0mm to 14.0mm, + + + 14.0mm to 18.0mm, + +++, greater than 18.0mm)

From these results, it was found that a culture of Lactobacillus plantarum CJLP133 strain has antibacterial activity and thus can be used for skin antibiosis.

From the foregoing, it will be appreciated by those skilled in the art to which the present application pertains that the present application may be embodied in other specific forms without modifying the technical concept or essential features of the application. In this regard, the exemplary embodiments disclosed herein are for illustrative purposes only and should not be construed to limit the scope of the present application. On the contrary, the application is intended to cover not only the exemplary embodiments, but also various alternatives, modifications, equivalents and other embodiments that may be included within the spirit and scope of the application as defined by the appended claims.

Registration number

The name of the depository institution: korea institute of bioscience and technology (KRIBB)

Registration number: KCTC 11403BP

The preservation date is as follows: 10 month and 9 days 2008

Claims

1. An antibacterial cosmetic composition for skin comprising a culture of Lactobacillus plantarum CJLP133 from which the bacterial bodies of Lactobacillus plantarum CJLP133 deposited under accession number KCTC 11403BP were removed.

2. The cosmetic composition according to claim 1,

wherein the culture is obtained by a method comprising:

(a) culturing the lactobacillus plantarum CJLP133 by inoculating the medium;

(b) centrifuging the resulting product of step (a); and

(c) recovering the supernatant from the resulting product of step (b).

3. The cosmetic composition according to claim 1,

wherein the culture is selected from the group consisting of Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli O157: h7, Pseudomonas aeruginosa and Propionibacterium acnes have antibacterial activity.

4. An antibacterial method for skin comprising administering to a subject a lactobacillus plantarum culture from which bacterial bodies of lactobacillus plantarum have been removed.

5. Use of a composition comprising a culture of lactobacillus plantarum having its bacterial bodies removed, for the preparation of a cosmetic product having antibacterial activity against the skin.