WO2019098752A1 - Antibacterial cosmetic composition comprising culture of lactobacillus plantarum - Google Patents

Abstract

The present application relates to an antibacterial cosmetic composition comprising a culture of Lactobacillus plantarum.

Description

Antibacterial cosmetic composition comprising Lactobacillus plantarum culture

The present invention relates to a cosmetic composition having an antibacterial effect containing a single lactic acid bacterium culture, and more particularly to a cosmetic composition for skin antibiosis comprising a culture of Lactobacillus plantarum CJLP133 .

Skin protects the body from various oxidants, air pollution, heavy metals, ultraviolet radiation (UV), etc. It prevents bacteria, fungus and virus from invading the skin and acts as a protective barrier against moisture loss. The structure of the skin is divided into three layers of epidermis, dermis and subcutaneous fat, and 90% of the dermis consists of collagen, elastin, hyaluronic acid and glycoprotein. Major components Collagen is an important fibrous protein that accounts for about 25% of the total protein mass of the human body. It occupies most of the connective tissue and functions as intercellular matrix, structural support function, cell division and differentiation induction, skin tensile strength Provide important functions such as providing.

In addition, sebum components, sweat components, and fatty acids, higher alcohols, and proteins contained in cosmetic ingredients, which are emitted from the body, are decomposed into substances that are highly toxic by the skin-damaging bacteria existing on the skin, thereby causing skin inflammation It is well known that exposure to secondary infection by pathogenic microorganisms caused by skin barrier damage caused by ultraviolet rays of the sun causes various skin diseases such as acne, itching, dermatitis and allergy.

Therefore, maximizing the effect of each ingredient and minimizing the side effects to prevent skin aging is a very important problem in cosmetics, which inhibits the growth of harmful bacteria, has no side effects, It is essential to develop cosmetics that are easy to use.

Lactic acid bacteria are beneficial in the human body to ferment carbohydrates and fiber to produce lactic acid (lactic acid). About 400 species have been found so far, of which 18 have been developed as products. When lactic acid bacteria enter the intestines of the human body, they secrete specific substances through their own metabolic activities. Since these substances have a beneficial effect on the human body, many studies on lactic acid bacteria have been made. Antimicrobial, antioxidant and immunological activities have been reported to date, and in recent years, whitening and moisturizing effects of lactic acid bacteria have been studied, and the possibility of becoming a flourishing material is increasing. Among the lactic acid bacteria, Lactobacillus has been reported to have a great effect on the immunity, antibacterial effect, skin whitening and atopic dermatitis.

Among them, POLAB (Plant Originated Lactic Acid Bacteria) is a lactic acid bacterium isolated from a fermented food of plant raw materials such as pickles, kimchi, and is referred to as an animal lactic acid bacteria. The vegetable lactic acid bacterium is more than 10 times more than the conventional animal lactic acid bacteria, and has excellent adaptability to the external environment. In particular, vegetable lactic acid bacteria isolated from kimchi, which have high salt content, low pH, and high content of natural antimicrobial substances, are produced in a harsh environment, and thus produce various physiologically active substances. The representative physiologically active substances include antimicrobial peptides, immunostimulating substances, and GABA (Gamma-Amino Butyric Acid), which exhibits anti-inflammatory and immunosuppressive effects among various physiological functions. Lactic acid bacteria also produce bacteriocins that act as antimicrobial peptides as heat-stable peptides. Bacteriocin is a natural antimicrobial protein or protein substance produced by microorganisms of various species, and generally refers to a substance having sterilizing activity against a species that is similar to the microorganism producing the same, or phylogenetically similar. Therefore, the development of cosmetic materials using the lactic acid bacteria culture fluid containing the above substances or the lactic acid bacteria fermentation plant extracts is active, which is in line with recent trends favoring environmentally friendly and safe substances.

Today, consumers are expected not only to expect general care effects on cosmetics, but also to have various functions such as anti-aging, anti-wrinkle, anti-irritation, anti-inflammatory and whitening effects. In recent years, various kinds of plant extracts have been used for the purpose of these cosmetics, and a new concept product is expected to show improved performance characteristics distribution by combining various various characteristics.

[Prior Art Literature]

[Patent Literature]

(Patent Document 1) KR 10-1486999 B1 (Jan. 21, 201)

In this application, we examined the effect of kimchi-derived Lactobacillus cultures derived from kimchi on the skin as a cosmetic product that can be safely used for anti-bacterial activity through antimicrobial activity, and to examine the possibility of domestic industrialization. Lactobacillus plantarum CJLP133 culture was found to have skin antimicrobial activity and the present application was completed.

One object of the present application is to provide a cosmetic composition for skin antibiosis comprising a Lactobacillus plantarum CJLP133 culture in which the cells of Lactobacillus plantarum CJLP133 deposited with the deposit number KCTC 11403BP are removed.

The present application provides a method for producing a lactobacillus plantarum culture which is safe without adverse effect on the skin by using a lactobacillus plantarum CJLP133 strain, which is a skin benefit bacterium, and which contains a culture of Lactobacillus plantarum CJLP133, To provide a cosmetic composition.

Figure 1 shows the results of the antibacterial activity test of the lactobacillus plantarum CJLP133 culture; 2a: Staphylococcus aureus , 2b: Staphylococcus epidermidis , 2c: E. coli O157: H7 , 2d: Pseudomonas aeruginosa , 2e: Propionibacterium acnes .

In one aspect for solving the above problems, the present application is directed to a method for the treatment of skin antimicrobials comprising Lactobacillus plantarum CJLP133 cultures from which the cells of Lactobacillus plantarum CJLP133 deposited with accession number KCTC 11403BP have been removed, The present invention provides a cosmetic composition.

Hereinafter, the antimicrobial cosmetic composition containing the lactobacillus plantarum culture in the present application will be specifically described.

The term " Lactobacillus plantarum " in the present application is one of microorganisms of the genus Lactobacillus, and is a gram-negative bacterium and a microorganism producing lactic acid. It is found in fermented food such as sour crout, pickle, kimchi.

The Lactobacillus plantarum strain may specifically be a strain of Lactobacillus plantarum CJLP133, which may be one deposited with the deposit number KCTC 11403BP.

Specifically, the Lactobacillus plantarum CJLP133 strain was deposited internationally on October 16, 2008 under the Budapest Treaty with the KCTC 11403BP as an international depositary to the KCTC, an international depository institution of the Korea Research Institute of Bioscience and Biotechnology.

The term " culture product " in the present application means a culture medium containing a by-product produced by culturing a strain in a culture medium and ingesting nutrients and metabolizing the culture medium. The culture medium containing the diluted solution, concentrate, , A preparation of the culture medium, a preparation of the culture medium or a mixture thereof, and a culture of all the formulations which can be formed using the culture medium.

Specifically, the culture may be a culture obtained by culturing a strain of Lactobacillus plantarum CJLP133, and may be obtained by removing the strain of the strain after culturing. That is, a culture medium in which the strain is removed from the strain after culturing the strain may be used as a culture.

More specifically, the culture comprises (a) inoculating and culturing Lactobacillus plantarum strain CJLP133 in a medium; (b) centrifuging the result of step (a); And (c) recovering the supernatant from the product of step (b).

In the step (a), the culture medium may be any medium used in the art, provided that the culture can be cultured and the culture after cultivation is obtainable. Specifically, the medium may be a liquid medium, more specifically, have.

The step (a) may further include a step of culturing the Lactobacillus plantarum strain in a solid medium and culturing the strain in the presence of the step (a) so as to obtain a purely isolated strain, thereby obtaining a confirmed colonies .

The step (b) may be carried out at a temperature of 1 ° C to 7 ° C, specifically 2 ° C to 6 ° C, more specifically 3 ° C to 5 ° C, using a high speed centrifuge at 1,500 rpm to 3,500 rpm, specifically 2,000 rpm to 3,000 rpm , More specifically, centrifuging at a rotational force of 2,200 rpm to 2,800 rpm for 10 minutes to 30 minutes, specifically 15 minutes to 25 minutes, more specifically 17 minutes to 23 minutes.

The step (c) may further comprise filtering the supernatant. The step (c) may further include a step of pulverizing the filtered supernatant using a freeze dryer.

The term " antibacterial " in the present application means the efficacy of inhibiting the growth of microorganisms such as skin disease-related bacteria.

In the present application, the culture is a skin disease-associated bacterium, specifically Staphylococcus aureus , Staphylococcus epidermidis , E. coli O157: H7 , Pseudomonas aeruginosa , But is not limited to, one or more bacteria selected from the group consisting of Propionibacterium acnes .

In addition, the composition of the present application may be to improve skin diseases caused by microorganisms through the above-mentioned antibacterial activity.

The term in this application. &Quot; Improvement " refers to the use of the composition of the present application to inhibit or inhibit the symptoms of skin diseases caused by bacteria or alleviate the symptoms of skin diseases already present.

In the present application, the cosmetic composition according to the present invention can be used as a cosmetic composition, a cosmetic composition, a cosmetic composition, a cosmetic composition, a cosmetic composition, a cosmetic composition, It may be formulated as a suspension, emulsion, paste, gel, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, patch and spray, It is not limited.

In the present application, the cosmetic composition may further comprise at least one cosmetically acceptable carrier mixed with a cosmetic composition for general skin. Examples of the cosmetic composition include ordinary components such as oil, water, a surfactant, a moisturizer, a lower alcohol , A thickening agent, a chelating agent, a coloring agent, a preservative, a flavoring agent, and the like, but is not limited thereto.

The cosmetically acceptable carrier contained in the cosmetic composition of the present application varies depending on the formulation of the cosmetic composition.

When the formulations of the present application are ointments, pastes, creams or gels, it is preferable to use an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracer, a cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide May be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present application is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder or the like may be used as a carrier component. But are not limited to, propellants such as rocaborn, propane / butane or dimethyl ether. These may be used alone or in combination of two or more.

When the formulation of the present application is a solution or emulsion, a solvent, a dissolving agent or an emulsifying agent may be used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Propylene glycol, 1,3-butyl glycol oil and the like can be used, and particularly fatty acid esters of cottonseed oil, peanut oil, corn oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or sorbitan May be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present application is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, but are not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present application is soap, an alkali metal salt of a fatty acid, a fatty acid hemiester salt, a fatty acid protein hydrolizate, an isethionate, a lanolin derivative, an aliphatic alcohol, a vegetable oil, a glycerol, a sugar and the like are used as a carrier component But is not limited thereto. These may be used alone or in combination of two or more.

Another aspect of the present invention provides a skin antimicrobial method comprising the step of administering to a subject a Lactobacillus plantarum culture in which the cells of the Lactobacillus plantarum have been removed.

The skin antimicrobial method may be a method comprising a step of administering a cosmetic composition further comprising a Lactobacillus plantarum culture and an cosmetically acceptable carrier to an individual in need of skin antibiosis, Acceptable carriers are the same as described above.

The subject may include a human, or may exclude a human. The subject may specifically include mammals, birds, etc., including cows, pigs, sheep, chickens, dogs, humans and the like. The subject having skin antimicrobial activity by the administration of the composition of the present invention is not limited.

Another aspect of the present invention provides a use of a composition comprising a lactobacillus plantarum culture in which a bacterium of the genus Latobacillus plantarum has been removed, for producing a cosmetic having skin antimicrobial activity do.

The present application will be described in more detail by the following examples. However, these embodiments are for illustrative purposes only, and the scope of the present application is not limited by these embodiments, and will be apparent to those skilled in the art to which the present application belongs.

Example 1 Preparation of Lactobacillus plantarum CJLP133 culture

The Lactobacillus plantarum CJLP133 strain deposited with accession number KCTC 11403BP was plated on MRS solid medium (BD Difco, Lactobacilli MRS Agar) and incubated at 37 ° C for about 18 hours. The colonies identified as pure isolates were platinum loops and inoculated into MRS liquid medium (BD Difco, Lactobacilli MRS Broth) and incubated at 37 ° C for approximately 18 hours. After cultivation, the concentration of the culture was 1.0 * 10 ⁹ CFU / mL. The culture broth was centrifuged at 4 ° C and 2,500 rpm for 20 min using a high-speed centrifuge (LABOGENE 1248R) to separate the cells from the culture. The supernatant of the culture was filtered, and the filtered supernatant was pulverized using a freeze dryer (HETO PL90000). The culture prepared by the above method was called " Lactobacillus plantarum CJLP133 culture ".

Example 2. Confirmation of the antibacterial activity of Lactobacillus plantarum CJLP133 culture

In order to confirm the antibacterial activity against the skin harmful bacteria of the lactobacillus plantarum CJLP133 culture prepared in Example 1, inhibition rings against skin harmful bacteria were measured by disc diffusion assay.

Staphylococcus aureus , Staphylococcus epidermidis , E. coli O157: H7 , and fungoides ( Pseudomonas aeruginosa ), which are commonly detected in the skin, , Acne bacteria ( Propionibacterium acnes ) were used. All five species were distributed at the Korea Biotechnology Research Center. Table 1 shows the details of the strain number, culture medium, culture temperature and incubation time for each strain.

Propionibacterium acnes was sealed in an anaerobic incubator using a gas pack system (MITSUBISHI GAS CHEMICAL, AnaeroPack) and anaerobically incubated.

Strain name	Access number	badge	Incubation temperature	Incubation time
Staphylococcus aureus	ATCC 25923	TSA	37 ℃	24 ~ 36hr
Staphylococcus epidermidis	KCTC 1917	TSA	37 ℃	24 ~ 36hr
Escherichia coli O157: H7 )	ATCC 25922	TSA	37 ℃	24 ~ 36hr
Pseudomonas aeruginosa	KCTC 1636	TSA	37 ℃	24 ~ 36hr
Propionibacterium acnes ( Propionibacterium acnes )	KCTC 3314	RCM	37 ℃	3 ~ 5days

As a control, MRS liquid medium was lyophilized and pulverized, and sterilized distilled water was added at a concentration of 0.5 g / mL. As a test group, materials prepared by adding Lactobacillus plantarum CJLP133 cultures to sterilized distilled water at concentrations of 0.1 g / mL, 0.5 g / mL, and 2.5 g / mL, respectively.

Each skin harmful bacterial strain was plated in 1.0 × 10 ⁶ CFU / mL in TSA (BD Difco, Tryptic Soy Agar, RCM; BD Difco Reinforced Clostridial Medium). A sterilized paper disc (ADVANTEC, 8 mm) was placed on a plated plate of each strain, and 50 μL of the test substance and the control substance were added. After culturing according to the culture conditions of each strain (Table 1), the diameter of the clear zone around the disc was measured. The greater the circle, the greater the antimicrobial activity. Without antimicrobial activity, the inhibitory ring is not produced.

As a result, no inhibitory ring was produced in the control group, and in the test group using the Lactobacillus plantarum CJLP133 culture, inhibitory rings were formed in all five strains, and the lactobacillus plantarum CJLP133 culture had antimicrobial activity against skin harmful bacteria . In addition, as the concentration of Lactobacillus plantarum CJLP133 culture increased, the size of the inhibitory loop was increased and the increase of the antimicrobial activity was confirmed in a concentration dependent manner. The experimental results are shown in Table 2 and Fig.

Strain name	Clear zone (mm)
	The control (MRS, 0.5 g / mL)	Concentration 1 (0.1 g / mL)	Concentration 2 (0.5 g / mL)	Concentration 3 (2.5 g / mL)
Staphylococcus aureus	-(0)	+ (2.0)	++ (10.0)	+++ (19.8)
Staphylococcus epidermidis (Staphylococcus epidermidis)	-(0)	-(0)	+ (6.0)	+++ (15.0)
Escherichia coli O157: H7 )	-(0)	+ (2.0)	+ (6.5)	+++ (14.5)
Pseudomonas aeruginosa	-(0)	+ (2.0)	+ (9.0)	+++ (16.8)
PROFIBUS sludge tumefaciens acne (Propionibacterium acnes)	-(0)	-(0)	++ (11.0)	+++ (18.3)

(-: no inhibition zone, +: less than 10.0 mm, ++: 10.0 to 14.0 mm, +++: 14.0 to 18.0 mm, ++++: more than 18.0 mm)

These results indicate that the culture of the Lactobacillus plantarum CJLP133 strain has antimicrobial activity and can be used for skin antimicrobial use.

From the above description, it will be understood by those skilled in the art that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. In this regard, it should be understood that the embodiments described above are illustrative in all aspects and not restrictive. The scope of the present application is to be interpreted as being within the scope of the present application, all changes or modifications derived from the meaning and scope of the following claims and equivalents thereof, rather than the detailed description.

[Access number]

Institution name: Korea Biotechnology Research Institute

Accession number: KCTC11403BP

Payment date: 20081009

Claims (5)

1. A Lactobacillus plantarum CJLP133 culture deposited with the deposit number KCTC11403BP, wherein the Lactobacillus plantarum CJLP133 cell culture is removed.
2. The method according to claim 1,

   The culture comprises (a) inoculating and culturing Lactobacillus plantarum strain CJLP133 in a medium; (b) centrifuging the result of step (a); And (c) recovering the supernatant from the product of step (b).
3. The method according to claim 1,

   The culture is Staphylococcus aureus (Staphylococcus aureus), Staphylococcus epidermidis (Staphylococcus epidermidis), E. coli (E. coli O157: H7), Pseudomonas aeruginosa (Pseudomonas aeruginosa) and propionyl sludge selected from the group consisting of tumefaciens acne (Propionibacterium acnes) Wherein the cosmetic composition has antimicrobial activity against at least one bacterium.
4. A method of skin antimicrobial treatment comprising administering to a subject a Lactobacillus plantarum culture in which the cells of the Lactobacillus plantarum have been removed.
5. Use of a composition comprising a Lactobacillus plantarum culture in which a bacterium of the genus Lactobacillus plantarum has been removed, for the production of a cosmetic having skin antimicrobial activity.

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