KR101835534B1 - Pharmaceutical Composition Having High Antibacterial Activity By Bioconversion Of Actinidia Arguta Stem Extract and Method For Manufacturing the same - Google Patents

Abstract

More particularly, the present invention relates to a biotransformation fruit tree extract having excellent antimicrobial activity through a biotransformation process with little antimicrobial activity in the state of the extracts of the seedlings and its preparation method.

Description

TECHNICAL FIELD The present invention relates to a method for producing a bacterium of the genus Bacillus subtilis, a method for producing the same, and a pharmaceutical composition comprising the extract,

The present invention relates to a technique relating to the improvement of antibacterial activity, more specifically, a method for producing the biotransformed tree extract having an antibacterial activity almost exclusively with antimicrobial activity but having an excellent antimicrobial activity through a biotransformation process, and a pharmaceutical ≪ / RTI >

Infectious diseases are collectively referred to as infectious diseases. Infectious diseases means that the pathogenic microorganisms enter and propagate in the living body. In addition, the infection caused by the bacterial proliferation in vivo is caused by the host resistance and pathogenicity It is known that it relies on the mutual relationship of microorganisms with their power. This mutual relationship is mainly caused by normal flora (normal flora), which means a microorganism that harmonizes between hosts. Normal bacterial flies act as host defense mechanisms. Among these microorganisms are beneficial bacteria known to be beneficial to host animals such as lactic acid bacteria, and harmful bacteria which are known to be potentially harmful to hosts such as Escherichia coli, Salmonella and Staphylococcus. Changes in the microbial flora of the host animal may be altered and destroyed by increased stress, infection by harmful bacteria, and changes in the external environment. In this case, the health of host animals deteriorates due to the rapid growth of harmful microorganisms. They prevent external pathogens from colonizing the skin or intestines and first place them on the skin to prevent growth and infection of other microorganisms.

The technology related to the enhancement of the antibacterial activity of domestic lactic acid bacteria is known by measuring the antibacterial activity of the lactic acid bacteria by isolating the lactic acid bacteria or by improving the antibacterial ability of the lactic acid bacteria using the bioconversion fermentation broth. In addition, the biotransformation technology using herbal medicines is relatively small. However, in the case of bioconversion using the herbal medicine extract, the useful components are extracted and the antibacterial power of the herbal medicine extract and the antibacterial activity by the bacteriocin produced by the lactic acid bacteria This can be seen as an effect of increasing the antibacterial activity.

As a patent for antibacterial activity using lactic acid bacteria, lactic acid bacteria Lactococcus lactis BH5 (KCM 10275), which produces an antimicrobial peptide substance of Patent No. 10-0413335-0000, and novel Lactobacillus bacteria of patent No. 10-1378673-0000, There are patents related to flutamide, a method for producing the same, and a composition for preventing or treating Escherichia coli diarrhea using the same. Examples of patents relating to biotransformation technology using lactic acid bacteria include Patent No. 10-1506995-0000, A patent on a cosmetic composition made of fermented water of Patent No. 10-1051535-0000, and the like.

Patent patents relating to antimicrobial activity using herbaceous plants include Patent No. 10-2006-0060906, a method for producing an antimicrobial shampoo having an antimicrobial activity against Staphylococcus aureus, a shampoo composition prepared by this method, A patent for a cosmetic composition containing a black rice, a black soybean, a black sesame, a mother wolf, an iris, a tea leaf, and a mixed extract of marigold.

On the other hand, Bower actinidia is a vine-bearing deciduous tree belonging to the genus Porphyra, and its scientific name is Actinidia arguta PLANCH. There are four kinds of plants in Korea (Dahlia, Dahlia, Dahlia, and Rat Dahlia) and they are widely grown throughout the country. They grow well in deep mountain forests, deep soils, and rich in humic substances. The length of the stem is 7m, the young branches have fine hairs, the leaves are wide, oval or oval. The flower is a two-year-old flower, bloomed in May, and 3 to 10 runs white. The fruit is egg-shaped, round in October with yellowish green color and tastes good. It is said that it is good for the neuralgia if it is dried for the month which dried by putting hot water and it is reproductive because it has sweetness. Especially, it is considered as a precious medicinal herb that is called Mukcheon Ryu (木 天 蓼), which has a chrysanthemum (lump-like plant) in the fruit. In the case of leaves and stems of P. vaginalis, a large amount of flavonoids, antioxidants, anticancer, antiviral, antiallergic and anti-inflammatory activities, which are antioxidants that prevent oxidation of free radicals and chronic diseases, And can meet the demand and demand for the above-mentioned antioxidants, and has advantages of relatively high safety and low side effects because it is a natural product. It is said that it has the effect on the body, such as diuretic, and it is effective for diuretic o gonorrhea. The bark and trunks of the seedlings are sometimes replaced by strings.

However, there has been no report on the fact that the extracts of P. vivax have antibacterial properties against pathogenic skin diseases. In particular, the antimicrobial properties against pathogenic skin diseases caused by the bioconversion of M. tuberosum extract using lactic acid bacteria have not been reported

The inventors of the present invention completed the present invention by developing a biotransformation fruit tree extract having an antibacterial activity against pathogenic skin disease causative microorganisms in the state of aquatic plant extract, but having an excellent antimicrobial activity against pathogenic skin disease causative bacteria through a bioconversion process.

Accordingly, an object of the present invention is to provide a biotransformation fruit tree extract having an excellent antimicrobial activity against a pathogenic skin disease causative microorganism obtained by biotransformation of an alopogwiazid extract which has no antimicrobial activity against pathogenic skin disease causative bacteria.

It is another object of the present invention to provide a microorganism which is capable of imparting excellent antimicrobial activity to a pathogenic skin disease microorganism to a seedling extract having almost no antibacterial activity against pathogenic skin diseases, The present invention provides a method for producing a biotransformed fruit tree extract having an excellent antibacterial activity against disease causative microorganisms.

It is another object of the present invention to provide a variety of application products such as a pharmaceutical composition containing a biodegradable alopogran tree extract having excellent antimicrobial activity against pathogenic skin diseases.

The objects of the present invention are not limited to the above-mentioned objects, and other objects not mentioned can be clearly understood by those skilled in the art from the following description.

In order to achieve the object of the present invention described above, the present invention provides a biotransformation fruit tree extract having antimicrobial activity against a skin disease causative microorganism.

In a preferred embodiment, the antimicrobial activity is imparted through conversion of the extract of Aloe vera extract to lactic acid bacteria.

In a preferred embodiment, the skin disease causative microorganism is at least one selected from the group consisting of purulent Streptococcus pyogenes , Propionibacterium acnes , and Streptococcus pyogenes .

In a preferred embodiment, the lactic acid bacteria used for said lactic acid bioconversion are It is Lactobacillus plantaurum .

In a preferred embodiment, the Lactobacillus plantaurm is L. plantarum 299V (DSM 9843).

In addition, the present invention provides a method for preparing a medicinal herb comprising the steps of: An inoculation step of inoculating a lactic acid bacterium into the liquid medium containing the extract of Purple Alaska Extract; And a culturing step of culturing the lactic acid bacterium in the inoculated state so as to carry out the bioconversion of the extract of P. japonicus extract to obtain a bioconversion culture.

In a preferred embodiment, the method further comprises a removing step of removing the lactic acid bacteria contained in the bioconversion culture.

In a preferred embodiment, the method further comprises a step of drying the bioconversion culture obtained in the cultivation step, or the step of removing the lactic acid bacteria contained in the bioconversion culture and the step of drying the bioconversion culture in which the obtained lactic acid bacteria have been removed .

In a preferred embodiment, the lactic acid bacteria inoculated in said inoculation step is Lactobacillus plantaurum .

In a preferred embodiment, the Lactobacillus Planta room (Lactobacillus plantaurm) is Lactobacillus plantaurm is L.plantarum 299V (DSM9843) and is inoculated in an amount of 1X10 ³ CFU / ml to 1X10 ⁸ CFU / ml.

In a preferred embodiment, the culturing step is carried out at a temperature range of 25 to 40 degrees for 12 to 96 hours.

In addition, the present invention relates to a method for preventing, preventing, or ameliorating an infectious disease caused by a pathogenic skin disease causative microorganism comprising, as an active ingredient, any one of the above- There is provided a pharmaceutical composition for improving or treating diseases.

In a preferred embodiment, the disease caused by pathogenic skin disease bacteria is one or more of laryngitis, tonsillitis, scarlet fever, hunger, sepsis, acne, sacchoidosis, uveitis, conjunctivitis, keratitis, skin rash, chronic inflammation and pyogenic infection to be.

The present invention also relates to a method for producing a health functional food having antimicrobial activity against a pathogenic skin disease causative microorganism, which comprises the above-described biotransformed fruit tree extract or the biotransformation tree extract prepared by any one of the above- .

The present invention also provides a method for treating an infectious disease caused by pathogenic skin disease causing bacteria, comprising the step of administering the above-mentioned pharmaceutical composition to a mammal other than a human.

The present invention also relates to a cosmetic composition having antimicrobial activity against a pathogenic skin disease causative microorganism which comprises, as an active ingredient, any one of the above-mentioned biotransformed Trichomon extracts or the above-described bioconverted Trichothecinus extract prepared by any one of the above- to provide.

First, the biotransduction tree extract of the present invention has excellent antimicrobial activity against pathogenic skin disease causative bacteria.

In addition, the method of the present invention for producing a biotransformed fruit tree extract comprises a step of inoculating a specific microorganism capable of imparting an excellent antimicrobial activity to pathogenic skin disease causative microorganisms, It is possible to produce a biotransformed seedling tree extract having excellent antimicrobial activity against causative bacteria of pathogenic skin diseases by culturing under constant culture conditions.

In addition, various application products such as the pharmaceutical composition including the biotransformation tree extract of the present invention include a biotransformation tree extract having an excellent antimicrobial activity against pathogenic skin diseases, thereby preventing the disease caused by pathogenic skin diseases , Has excellent antimicrobial activity for improvement and treatment.

Fig. 1 is a photograph of a blueberry tree.
FIG. 2 is a graph showing changes in pH of the extracts of Bombyx mori, Bryophyta, and Bifidobacterium, obtained in the examples of the present invention.
FIG. 3 is a graph showing antimicrobial activity against the causative microorganisms of skin diseases of the extracts of P. fluorescens and P. multocida obtained in the examples of the present invention.
Fig. 4 is a photograph showing the antimicrobial activity against the causative bacteria of the skin diseases of the extracts of Bifidobacterium tuberosum extract and Bifidobacterium tuberosus extract obtained in the examples of the present invention.

Although the terms used in the present invention have been selected as general terms that are widely used at present, there are some terms selected arbitrarily by the applicant in a specific case. In this case, the meaning described or used in the detailed description part of the invention The meaning must be grasped.

Hereinafter, the technical structure of the present invention will be described in detail with reference to the accompanying drawings and preferred embodiments.

However, the present invention is not limited to the embodiments described herein but may be embodied in other forms. Like reference numerals used to describe the present invention throughout the specification denote like elements.

The technical feature of the present invention is that there is little antimicrobial activity against pathogenic skin disease causative microorganisms in the state of the extract of Alnus davidiana, but the biotransformed alfalfa extract obtained through the bioconversion process has an excellent antimicrobial activity against pathogenic skin disease causative bacteria Which has excellent antimicrobial activity against causative bacteria of pathogenic skin diseases, and a method for producing the same. The present invention also provides a pharmaceutical composition, a health functional food, and a cosmetic composition which are capable of preventing, improving or treating various infectious diseases using characteristics having an excellent antimicrobial activity against pathogenic skin disease causative bacteria which can not be obtained from a simple purple tree extract.

Here, bioconversion refers to a technique of producing and manufacturing various metabolites using enzymes or microorganisms, and it is possible to produce a useful substance by fermentation under anaerobic conditions or micro-aerobic conditions of microorganisms It is the technology that changes. Bioconversion is being carried out using various materials such as plant, herbal medicines, feed, and silk. It is known that there are various effects of biochemical conversion of herbal medicine using microorganisms. Microbial biotransformation is a characteristic feature of the decrease in the size of the useful substance and the improvement of the function. Production of useful substances such as vitamins, organic acids and bacteriocins produced by the microorganisms also serves as an advantage of biotransformation.

Therefore, the biotransduction tree extract of the present invention has antimicrobial activity against the causative bacteria of skin diseases.

The causative bacteria of skin diseases may be one or more of purulent Streptococcus pyogenes , Propionibacterium acnes , and Streptococcus pyogenes , which are known to be causative agents of skin diseases. Streptococcus pyogenes is known to be a cause of human laryngitis, tonsillitis, scarlet fever, hunger, and sepsis. Propionibacterium acnes is a pathogenic bacterium present in the skin and intestinal tract. It causes acne, squid, uveitis, conjunctivitis, keratitis, and causes skin rash and chronic inflammation. Staphylococcus aureus is an important cause of skin infection in atopic patients. It is characterized by secretion of toxins that kill phagocytes such as hemolysin, coagulase, and lucosidine, leading to a pyogenic infectious disease. Propionibacterium acnes exists in the skin or sebaceous glands and is characterized by massive proliferation when the sebaceous glands are active due to increased adolescent hormone activity. When acne is caused by Propionibacterium acnes in adolescent atopic patients, symptoms such as dry skin and increased skin erythema accompanied by itching will appear. In addition, Staphylococcus aureus and Streptococcus pyogenes The symptoms of acne due to tea bacterium infection worsen. According to Yoon et al. (2009), secondary infection of Streptococcus pyogenes was found in atopic patients with fever, purulent exudates, pustules, pustules, etc. and symptoms of sepsis due to bacterial infection were confirmed. In addition, antibiotics, antihistamines, and topical steroids are used to prevent secondary bacterial infections. Streoid phobia causes side effects of steroids. It is known that Staphylococcus aureus is not only the primary cause of atopic dermatitis but also a secondary cause of infections such as skin pore infections, sweat gland blockage, and wound infections. In 90% of atopic patients, Infection of S. aureus has been confirmed. In addition, it suggests that using Oriental medicine can inhibit secondary infection of Streptococcus pyogenes and Staphylococcus aureus .

The antimicrobial activity of the biotransformed seedling extract of the present invention is conferred through the conversion of the extract of Aloe vera extract to the microbial biosynthesis of lactic acid bacteria, and the lactic acid bacterium used for microbial transformation of the microbes may be Lactobacillus plantaurum . In particular, L. plantarum 299V (DSM 9843), L. plantarum 299V was first isolated in fermented food in 1994, and has been studied in irritable bowel syndrome, mucosal inflammation and the like.

The pH of the bacterium extract of the present invention is 4 or less, which is comparable to that of the MRS medium, as compared to the pH of the extract of P. japonicus before the bioconversion. have.

Next, the method for producing a bioconverted Daru tree extract according to the present invention comprises: preparing a medium for preparing a liquid medium containing Extract of Mulberry tree by adding a Mulberry tree extract to a lactic acid bacterium culture composition; And an inoculation step of inoculating a lactic acid bacterium into the liquid medium containing the extract of Purple Alaska Extract; And a culture step of culturing the lactic acid bacterium so as to carry out the biotransformation of the plantain extract to obtain a bioconversion culture. The method may further comprise, if necessary, a removal step of removing the lactic acid bacteria contained in the biotransformation culture, Drying the transformed culture, removing the lactic acid bacteria contained in the biotransformation culture, and drying the biotransformation culture from which the obtained lactic acid bacteria have been removed.

Inoculation step is Lactobacillus Lactobacillus plantaurm of Planta room (Lactobacillus plantaurm) can be carried out by inoculating the L.plantarum 299V (DSM9843) in an amount of ^{1X10 3 CFU / ml 1X10 8 CFU} / ml.

The culturing step may be carried out at a temperature range of 25 to 40 degrees for 12 to 96 hours, but it may be shortened to 24 hours or less.

The drying step includes a freeze-drying step, which may be carried out at -80 占 폚 or lower for 36 to 72 hours.

Next, the pharmaceutical composition, health functional food and cosmetic composition for preventing, ameliorating or treating infectious diseases caused by pathogenic skin disease causative microorganism of the present invention include a biodegradable seedling extract as an active ingredient. As a result, the pharmaceutical composition of the present invention can treat infectious diseases induced by oral or parenteral administration for the treatment of infectious diseases caused by causative bacteria of pathogenic skin diseases of mammals other than humans.

The pharmaceutical compositions of the present invention may comprise a pharmaceutically acceptable carrier. Compositions comprising a pharmaceutically acceptable carrier may be in the form of tablets, pills, powders, granules, capsules, suspensions, solutions, emulsions, syrups, sterile aqueous solutions, nonaqueous solutions, suspensions, emulsions, lyophilized preparations, Oral or parenteral formulations selected from the group consisting of In the case of formulation, a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant is usually used. Solid formulations for oral administration include tablets, pills, powders, granules, capsules and the like, which may contain at least one excipient such as starch, calcium carbonate, sucrose or lactose, gelatin, . In addition to simple excipients, lubricants such as magnesium stearate, talc, and the like may also be used. Liquid preparations for oral administration include suspensions, solutions, emulsions, syrups and the like. Various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included in addition to water and liquid paraffin, which are simple diluents commonly used. have. Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Examples of the non-aqueous solvent and the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, and injectable ester such as ethyl oleate. Examples of the suppository base include witepsol, macrogol, tween 61, cacao paper, laurin, glycerogelatin and the like.

In the present invention, the extract of Purple-berry beetle used for biological conversion may be one obtained by extracting at least one of the branches, bark, leaves and fruits of the berry tree with water, C1 to C2 lower alcohol or a mixture thereof as a solvent. The seedling extract used in the bioconverted fruit tree extract of the present invention may be water, ethanol or a mixture thereof extracted as a solvent.

Example 1

1. Badge preparation step

① Preparing the extract

100 g of dried persimmon and 1 L of water are placed in a hot water bath and hydrothermal extraction is carried out. Extracts were extracted with 500 ml of hot water extracts and extracted with impurities using a paper filter.

② MRS broth powder, which is a lactic acid bacteria medium, was dissolved in the extract of Mulberry tree extract by adding 55 grams of MRS broth powder per 1 liter of extract of Mulberry tree extract, and sterilized.

2. Inoculation phase

Lactobacillus lactis Bacillus planta 299V (DSM9843) was inoculated to MRS medium containing Mulberry tree extract at a concentration of 1 × 10 ⁶ CFU / ml.

3. Culture step

Lactic acid bacteria were cultivated at 37 캜 for 24 hours so as to carry out bioconversion of the seedlings extract to obtain a biotransformed culture of the extracts of Mulberry tree.

4. Removal steps

The biotransformed cultures were centrifuged at 7000 rpm for 30 minutes and filtered using a 0.45 ㎛ syringe filter to remove the lactic acid bacteria contained in the biotransformed cultures.

Example 2

The biotransformed seedling extract was prepared in powder form by further performing a drying step comprising the step of freeze-drying the biotransformed fruit tree extract obtained in Example 1 at -70 ° C for 36 hours to 72 hours.

Experimental Example 1

The pH of the biotransformed Trichomonas sphaeroa extract after the bioconversion using the Purple Tree extract and the Lactobacillus obtained in Example 1 was measured and the results are shown in Table 1 and FIG. As shown in Table 1 and FIG. 2, the pH of the extract of Mulberry tree before the bioconversion was pH 6.2, and the pH of the control MRS broth and the bioconverted Mulberry tree extract were 3.9 and 3.98, respectively. .

Experimental Example 2

The antimicrobial effect of the cultured liquid of lactic acid bacteria, the extract of Purple alder tree obtained in Example 1 and the extract of biotransformed Daru tree (liquid) and the concentrate of biotransformed Daru tree extract (powder) obtained in Example 2 was confirmed as follows. At this time, the biotransformant extract (powder) was concentrated 5 times and used for the antimicrobial activity test after controlling with sodium acetate (pH 4.0) buffer solution.

Here, Lactobacillus plantarum 299V was cultured in MRS medium and Mulberry tree extract MRS medium. The pathogens used were confirmed to have antimicrobial inhibitory effects on three species of Propionibacterium acnes KCTC 3314, Streptococcus pyogenes KCCM 11873 and Staphylococcus aureus KACC 13236 . Streptococcus pyogenes KCCM 11873 was cultivated in a medium containing 5% Sheep blood in a TS (Tryptic-Soy) medium, in a medium of Staphylococcus aureus KACC 13236 was cultured in a TS (Tryptic-Soy) medium.

Specifically, the paper disc method was used to investigate the antibacterial activity. The biotransformed fruit juice extract, which is concentrated 5 times by 100 μl on 8 mm paper discs, is added to the 6 mm paper disc in a medium containing 1 × 10 ⁶ CFU / ml of each pathogenic bacterium. After 20 쨉 l of spotting, the medium was applied to a medium in which pathogens were stained. The medium on which the paper discs were germinated was cultured for 24 hours at 37 ° C to confirm whether or not growth inhibition rings were produced. The results are shown in Tables 2, 3 and 4

As can be seen from Table 2, FIG. 3 and FIG. 4, the antimicrobial activity was not confirmed in all the skin disease causative bacteria in the case of the extract of mulberry tree, and the antimicrobial activity was confirmed in S. aureus in the MRS culture solution of lactic acid bacteria. The antimicrobial activity of all the skin disease causative organisms was confirmed in the biotransformation tree extract, and the antimicrobial activity of S. aureus was increased by 13%. In the case of the biotransformation tree extract, the antimicrobial activity was increased from at least 6% to at most 18% before the concentration.

The results of the experiment that the extracts of the mulberry tree extract have antimicrobial activity against all the skin disease causative bacteria, but the biotransformation tree extract has antimicrobial activity against all the skin disease causative bacteria. It is possible to confirm that this is due to bioconversion.

From the above experimental results, it was confirmed that the biotransformation tree extract of the present invention can be used as a functional food material or a cosmetic material as well as a novel pharmaceutical composition for prevention and symptom improvement of infectious diseases caused by pathogenic skin disease causative bacteria. Particularly, the above experimental example shows the antibacterial activity of the causative agent of the skin disease causative microorganism of the bacterium transformed with Lactobacillus acidus using lactic acid bacterium. Among the causative strains showing the antimicrobial activity of the biotransformed Trichoderma extract of the present invention, It can be used as a composition for treating atopic pruritus, cosmetics for alleviating atopic pruritus such as lotion, skin, etc., by using the biotransformed fruit tree extract of the present invention.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is clearly understood that the same is by way of illustration and example only and is not to be taken by way of limitation, Various changes and modifications will be possible.

Claims (16)

delete delete delete delete delete Preparing a medium for preparing a liquid medium containing an extract of Mulberry tree by adding the extract of Mulberry tree to a lactic acid bacterium culture composition;
An inoculation step of inoculating a lactic acid bacterium into the liquid medium containing the extract of Purple Alaska Extract; And
Culturing the lactic acid bacterium in such a manner that the transformed tree extract is bioconverted in the inoculated state to obtain a bioconversion culture,
Wherein the culturing step is carried out at a temperature ranging from 25 캜 to 40 캜 for from 12 hours to 96 hours and the pH of the bioconversion culture is 4 or less.
delete The method according to claim 6,
Drying the bioconversion culture obtained in the culturing step, removing the lactic acid bacteria contained in the biotransformation culture, and drying the biotransformation culture in which the lactic acid bacteria have been removed. Wherein said method comprises the steps of:
The method according to claim 6,
Wherein the lactic acid bacteria inoculated in said inoculation step is Lactobacillus plantaurum .
10. The method of claim 9,
Wherein said Lactobacillus plantaurm is L. plantarum 299V (DSM 9843) and said Lactobacillus plantaurm is inoculated in an amount of 1 X 10 ³ CFU / ml to 1 X 10 ⁸ CFU / ml. .
delete The antimicrobial activity of at least one of Streptococcus pyogenes , Propionibacterium acnes , and Streptococcus aurei is imparted through the biotransformation of Lactobacillus sp . Is less than or equal to 4, or a bacterium selected from the group consisting of Streptococcus pyogenes containing an effective amount of a biotransformed fruit tree extract prepared by the method of any one of claims 6 to 8, A pharmaceutical composition for preventing, ameliorating or treating an infectious disease caused by pathogenic skin disease causative bacteria of one or more of Propionibacterium acnes and Streptococcus auresu . delete The antimicrobial activity of at least one of Streptococcus pyogenes , Propionibacterium acnes , and Streptococcus aurei is imparted through the biotransformation of Lactobacillus sp . Is less than or equal to 4, or a bacterium selected from the group consisting of Streptococcus pyogenes containing an effective amount of a biotransformed fruit tree extract prepared by the method of any one of claims 6 to 8, Propionibacterium acnes and Streptococcus auresu , a health functional food having antimicrobial activity against pathogenic skin diseases.
Treatment of an infectious disease caused by one or more pathogenic skin diseases selected from the group consisting of Streptococcus pyogenes , Propionibacterium acnes and Streptococcus auresu comprising the step of administering the pharmaceutical composition of claim 12 to a mammal other than a human. Way.
The antimicrobial activity of at least one of Streptococcus pyogenes , Propionibacterium acnes , and Streptococcus aurei is imparted through the biotransformation of Lactobacillus sp . Is less than or equal to 4, or a bacterium selected from the group consisting of Streptococcus pyogenes containing an effective amount of a biotransformed fruit tree extract prepared by the method of any one of claims 6 to 8, A cosmetic composition having antimicrobial activity against at least one pathogenic skin disease causative bacteria among Propionibacterium acnes and Streptococcus auresu .

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