TWI686209B - Antibacterial cosmetic composition comprising lactobacillus plantarum culture - Google Patents

Abstract

The present application relates to an antibacterial cosmetic composition for skin comprising a Lactobacillus plantarum culture.

Description

Antibacterial cosmetic composition containing embryonic lactobacillus culture

The present invention relates to a cosmetic composition having an antibacterial effect, which contains a single lactic acid bacteria culture, and in particular, to an antibacterial cosmetic composition for skin containing a culture of Lactobacillus plantarum CJLP133.

Human skin can protect the body from various oxides, air pollution, heavy metals and ultraviolet (UV) damage, and can prevent bacteria, fungi and viruses from invading the skin, and plays a role as a protective barrier against water loss. The structure of the skin is roughly divided into three layers of epidermis, dermis and subcutaneous fat, and the dermis layer contains 90% of the skin and is composed of collagen, elastin, hyaluronic acid and glycoprotein. Collagen is the main component of the skin and is an important fibrous protein, accounting for about 25% of the total weight of human protein. Collagen constitutes most of the connective tissue and plays important roles such as intercellular matrix function, structural support function, induce cell division and differentiation, provide skin tensile strength and so on.

In addition, it is known that fatty acids, higher alcohols, proteins, etc. in sebum and sweat components released from the body, and cosmetic components are decomposed into toxic substances by the skin flora present on the skin, causing skin inflammation; further, due to the sun The ultraviolet rays of light damage the skin barrier, and secondary infections caused by exposure to pathogenic microorganisms induce various skin diseases (eg, acne, itching, dermatitis, allergies, etc.).

Therefore, it is a very important issue to maximize the effect of each ingredient in cosmetics and minimize its side effects to prevent skin aging. In this regard, it is necessary to develop a cost-effective material that inhibits the growth of harmful bacteria without side effects, and a cosmetic that contains the material and is convenient to use.

Lactic acid bacteria are bacteria that produce lactic acid through the fermentation of carbohydrates or fiber and have beneficial effects in the human body. So far, about 400 kinds of lactic acid bacteria have been discovered, and about 18 varieties have been developed into commercial products.

Once lactic acid bacteria enter the intestinal tract of the body, they secrete specific materials through their own metabolic activity. Because these materials are beneficial to the human body, many studies have been conducted on lactic acid bacteria. The effects of lactic acid bacteria on antibacterial, antioxidant and immune activity have been reported. In recent years, research has focused on the whitening and moisturizing effects of lactic acid bacteria, and the possibility of use as a cosmetic material has increased. Among the lactic acid bacteria, many studies have been conducted on the effects of Lactobacillus, and immunity, antibacterial effects, enhancement of skin whitening, improvement of atopic dermatitis, etc. have been reported.

Among them, plant-derived lactic acid bacteria (POLAB) are lactic acid bacteria isolated from fermented foods of plant materials (such as pickles, kimchi, etc.), and are different from dairy-derived lactic acid bacteria.

Compared with animal lactic acid bacteria, lactic acid bacteria of plant origin not only include at least 10 times more types, but they also have excellent adaptability to the external environment. Especially the plant-derived lactic acid bacteria isolated from kimchi, which have high content of salt, low pH and high content of natural antibacterial materials, can grow in extreme environments, so plant-derived lactic acid bacteria have excellent productivity of a variety of different physiologically active materials .

Representative physiologically active materials include antimicrobial peptides, immunostimulants, γ-aminobutyric acid (GABA), and its performance includes various physiological functions such as anti-inflammatory and immunosuppressive effects. In addition, lactic acid bacteria are heat-stable peptides and produce bacteriocins as antibacterial agents. Bacteriocins are natural antimicrobial proteins or protein materials produced by various microorganisms, and are generally referred to as materials having a sterilization mechanism against bacterial strains that are similar in morphology and pedigree to bacteriocin-producing microorganisms. Therefore, cosmetic materials using lactic acid bacteria cultures or lactic acid bacteria fermented plant extracts containing these materials are being actively developed, and this progress is in line with the recent trend in favor of environmentally friendly and safe materials.

At present, consumers not only expect general care effects in cosmetics, but also various functions (eg, anti-aging, improved fine lines, anti-irritation, anti-inflammatory, whitening effects, etc.).

In recent years, based on the above purpose, various plant extracts have been used in cosmetics, and expectations for products based on new concepts are increasing. The new concept shows improved performance characteristics by combining various characteristics range.

[Prior Technical Literature] [Patent Literature]

[Patent Literature 1] KR10-1486999 B1 (2015.01.21)

In order to comply with the era of environmental protection, the inventors of the present invention have worked hard to study cosmetics from the culture of lactic acid bacteria of kimchi, which is safe for the skin without any side effects, and can be effectively used for antibacterial skin care via antibacterial activity. In addition, We must also study the possibility of its development in domestic industrialization. As a result, the inventors have confirmed that the culture of Lactobacillus germs CJLP133 has antibacterial activity on the skin, thus completing the content of the present invention.

The object of the present invention is to provide an antibacterial cosmetic composition for skin containing a culture of Lactobacillus germs CJLP133 deposited under the accession number KCTC 11403BP, in which the bacterial body of the Lactobacillus germs CJLP133 is removed.

The present invention provides a method for preparing a Lactobacillus germ L. germ CJLP133 culture using a Lactobacillus germ strain (ie, beneficial skin bacteria), which is safe for the skin and does not have any side effects, and also provides a cosmetic composition with antibacterial activity, It contains a Lactobacillus germ CJLP133 culture.

Figure 1 shows the experimental results of the antibacterial activity of L. germ CJLP133 culture. 2a: Staphylococcus aureus, 2b: Staphylococcus epidermidis, 2c: Escherichia coli O157: H7 type, 2d: Pseudomonas aeruginosa, and 2e: Propionibacterium acnes.

To achieve the above object, one aspect of the present invention is to provide an antibacterial cosmetic composition for skin containing a culture of Lactobacillus germs CJLP133 deposited under the accession number KCTC 11403BP, in which the bacterial bodies of the Lactobacillus germs are removed.

In the following, the antibacterial cosmetic composition for skin containing a culture of Lactobacillus germs provided by the present invention is explained in detail.

As used herein, the term "Lactobacillus germ" refers to a microorganism of the genus Lactobacillus, which is a Gram-negative bacterium capable of producing lactic acid. The bacterial system can be found in fermented foods such as sauerkraut, pickles, and kimchi.

The Lactobacillus germ strain, in particular the Lactobacillus germ strain CJLP133, is a strain deposited with accession number KCTC 11403BP.

Specifically, the Lactobacillus germ CJLP133 strain was deposited with the accession number KCTC 11403BP at the Korea Microbiological Preservation Center (KCTC) of the Korea Institute of Life Sciences and Biotechnology (KRIBB) on October 16, 2008. The organization is under the Budapest Treaty International depository.

As used herein, the term "culture" refers to a culture medium containing by-products produced when culturing a microorganism strain in a culture medium, wherein the strain allows ingestion and metabolism of nutrients contained in the culture medium. The term "culture" includes all formulations that can be formed using a culture medium (for example, a dilution or concentrate of the culture medium, a dried product obtained by drying the culture medium, a crude or purified product of the culture medium, a mixture of these, etc.).

Specifically, the culture may be obtained by cultivating the Lactobacillus germ L. strain CJLP133, or may be obtained by culturing the strain, wherein the bacterial body is removed after incubation. That is, the medium in which the bacterial body is removed can be used as a culture.

More specifically, the culture can be obtained by a method including: (a) culturing the Lactobacillus germ L. CJLP133 strain by inoculating a medium; (b) centrifuging the product obtained in step (a); and (c) from ( b) The product obtained in the step is recovered to Shangcheng part.

In the step (a), the medium may be any medium used in the art, as long as the medium can cultivate strains, and the culture is recovered after the cultivation, specifically a liquid medium, more specifically an MRS liquid medium.

In order to obtain a pure isolated strain, step (a) may further include a step of culturing and collecting colonies of the embryo Lactobacillus strains on a solid medium before step (a), wherein the colonies are confirmed to be pure isolated strains.

In step (b), a high-speed centrifuge is used to centrifuge at 1°C to 7°C, specifically 2°C to 6°C, more specifically 3°C to 5°C; the rotation rate is 1,500 rpm to 3,500 rpm, specifically 2,000 rpm to 3,000 rpm, more specifically 2,200 rpm to 2,800 rpm; performed for 10 to 30 minutes, specifically 15 to 25 minutes, more specifically 17 to 23 minutes.

Step (c) may further include the step of filtering the Shangcheng portion. In addition, step (c) may further include another step of powdering the filtered supernatant liquid using a freeze dryer.

In this context, the term "antibacterial" refers to the effect of inhibiting the growth of microorganisms (eg, bacteria associated with skin diseases).

In the present invention, the culture may have antibacterial activity against at least one bacterium selected from the group consisting of Staphylococcus aureus, Staphylococcus epidermidis, E. coli O157: H7, Pseudomonas aeruginosa, and Propionibacterium acnes, but Not limited to these.

In addition, through the antibacterial activity, the composition of the present invention can improve the skin diseases induced by microorganisms.

As used herein, the term "improvement" refers to the use of the composition of the present invention to suppress or suppress the symptoms of skin diseases caused by bacteria, or to alleviate the symptoms of skin diseases that have already appeared on the skin.

In the present invention, the cosmetic composition can be formulated into the following forms: solution, external ointment, cream, foam, nutritional skin cream, soft skin cream, dressing, soft water, lotion, primer, fragrance, soap, liquid cleanser , Bathing agent, sunscreen, sunscreen oil, suspension, emulsion, paste, gel, lotion, powder, soap, detergent with surfactant, oil, powder foundation, emulsified foundation, wax foundation, paste Tablets and sprays, but the formulations are limited to these.

In the present invention, the cosmetic composition may further contain at least one cosmetically acceptable carrier, which is used to add general skin cosmetics. As a conventional component of a cosmetically acceptable carrier, for example, oil, water, surfactants, humectants, lower alcohols, thickeners, chelating agents, colors, preservatives, perfumes, etc. can be appropriately mixed, but the component is not Subject to these.

The cosmetically acceptable carrier added to the cosmetic composition of the present invention can be changed according to the formulation of the cosmetic composition.

When the formulation of the present invention is an ointment, paste, cream or gel, etc., as the carrier component, animal oil, vegetable oil, wax, paraffin, starch, aspartame, cellulose derivative, polyethylene glycol, silicon Oxygen finger, bentonite, silica, talc, zinc oxide, etc., but the carrier component is not limited to these. The carrier component may be used alone or in combination of two or more.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, etc. can be used as a carrier component; especially when a spray is used, the formulation The substance may further contain propellants such as chlorofluorocarbons, propane/butane and dimethyl ether, but the composition of the carrier is not limited to these. The carrier component can be used alone or in combination of two or more.

When the formulation of the present invention is a solution or an emulsified product, a solvent, co-solvent or emulsifier (for example, water, ethanol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzene) can be used as a carrier composition Benzyl formate, propylene glycol, 1,3-butanediol oil, etc.), and especially cottonseed oil, peanut oil, cornseed oil, olive oil, castor oil, sesame oil, glycerin aliphatic ester, polyethylene glycol or dehydrated Sorbitol fatty acid ester, but the carrier component is not limited to these. The carrier component can be used alone or in combination of two or more.

When the formulation of the present invention is a suspension, as the carrier component, a liquid diluent (for example, water, ethanol, or propylene glycol), a suspending agent (for example, ethoxylated isostearyl alcohol, sorbitol polyoxyethylene ester, and Sorbitan polyoxyethylene ester), microcrystalline cellulose, aluminum oxyhydroxide, bentonite, agar, aspartame, etc., but the carrier component is not limited to these. The carrier component can be used alone or in combination of two or more.

When the formulation of the present invention is soap, as the carrier component, alkali metal salts of fatty acids, half-ester salts of fatty acids, fatty acid protein hydrolysates, isethionic acid, lanolin derivatives, aliphatic alcohols, vegetable oils can be used , Glycerin, sugars, etc., but the carrier component is not limited to these. The carrier component can be used alone or in combination of two or more.

Another aspect of the present invention is to provide an antibacterial method for skin, which method is to apply an Lactobacillus germ of embryo culture in which a bacterial body is removed to an individual.

The antibacterial method for skin may include applying a cosmetic composition containing an Lactobacillus germ culture to an individual in need of antibacterial skin, and additionally a cosmetically acceptable carrier. The cosmetically acceptable carrier is as described above.

Individuals may or may not include humans. Specifically, the individual may be a mammal, including cows, pigs, sheep, chickens, dogs, humans, etc.; birds, etc., and may include, but is not limited to, any individual who has antibacterial activity by administering the above composition.

Another aspect of the present invention is to provide the use of a composition for preparing an antibacterially active cosmetic containing a culture of Lactobacillus germ, wherein the bacterial body of the Lactobacillus germ is removed.

Hereinafter, the content of the present invention will be disclosed in more detail with reference to the following examples. However, for those skilled in the art, these embodiments are for illustrative purposes only, and the present invention is not intended to be limited to these embodiments.

Example 1: Preparation of LJ germ CJLP133 culture

The Lactobacillus sp. CJLP133 strain deposited under the accession number KCTC 11403BP was plated on a solid MRS medium (BD Difco, Lactobacillus MRS agar), and cultured at 37°C for about 18 hours. After confirmation, pure isolated colonies were collected and inoculated into a liquid MRS medium (BD Difco, Lactobacillus MRS broth) using a platinum ring, and cultured at 37°C for about 18 hours. After the cultivation is completed, the culture concentration is 1.0×10 ⁹ CFU/mL. In order to separate the bacterial body from the culture, the culture was centrifuged at 4°C and 2,500 rpm for 20 minutes using a high-speed centrifuge (LABOGENE 1248R). After filtering the upper portion of the culture, the filtered upper portion was powdered using a freeze dryer (HETO PL90000). The culture prepared by this method is called "Lactobacillus germ L. CJLP133 culture"

Example 2: Confirmation of the antibacterial activity of the Lactobacillus germ CJLP133 culture

In order to confirm the antibacterial activity of the Lactobacillus sp. CJLP133 culture prepared in Example 1 against skin pathogens, the inhibition circle of the skin pathogens was measured by the disk diffusion method.

A total of five skin pathogen strains are used, Staphylococcus aureus, Staphylococcus epidermidis, E. coli O157: H7 type, Pseudomonas aeruginosa and Propionibacterium acnes, which are often detected in the skin. All five strains of skin pathogens come from the Korea Microbiological Preservation Center (KCTC) of the Korea Institute of Life Science and Biotechnology (KRIBB). The details of the accession number, culture medium, culture temperature and incubation time of each strain are shown in Table 1 below.

Using a gas packaging system (Mitsubishi Gas Chemical Co., Ltd., anaerobic gas-producing bag), P. acnes was sealed in an anaerobic culture tank and cultivated anaerobicly.

As a control group, a material system was prepared by pulverizing a liquid MRS medium via a freeze-drying method and adding sterile distilled water to a concentration of 0.5 g/mL. As a test group, the materials used were prepared by adding sterile distilled water to the culture of Lactobacillus germ L. CJLP133 to a concentration of 0.1 g/mL, 0.5 g/mL, and 2.5 g/mL.

Each strain of skin pathogen was inoculated in a medium (TSA; BD Difco, tryptic soy agar, RCM; BD Difco, enhanced Clostridium medium) at a concentration of 1.6×10 ⁶ CFU/mL. After placing a sterile paper disc (ADVANTEC, 8 mm) on a plate in which each strain was cultured on a plate, 50 μL of the test material and the control material were added respectively. After cultivating each strain according to the respective culture conditions (Table 1), the diameter of the inhibition circle around the disc (ie, the area of apoptosis indicating the strain) was measured. A larger inhibition circle indicates that the antibacterial activity is better, and the inhibition circle will not be formed without antimicrobial activity.

The test results showed that the control group did not form an inhibitory circle, while the test group using the Lactobacillus germs CJLP133 culture formed inhibition circles in all five strains, thus confirming that the Lactobacillus germs CJLP133 culture had antibacterial activity against skin pathogens. In addition, as the concentration of L. germ CJLP133 culture increased, the size of the suppression circle also increased, confirming that the antibacterial activity increased in a concentration-dependent manner. The test results are shown in Table 2 and Figure 1.

Based on these results, it can be found that the culture of the Lactobacillus germ L. CJLP133 strain has antibacterial activity, and thus can be used for skin antibacterial.

Those skilled in the art of the present invention can understand from the foregoing content that the present invention can be modified by other specific forms without changing the technical concept or essential characteristics of the content disclosed in this street. In this regard, the illustrative aspects disclosed herein are for illustrative purposes only and should not be construed as limiting the scope of this disclosure. On the contrary, this disclosure tends to cover not only these exemplary features, but also various changes, modifications, equivalents, and the like that can be included within the spirit and scope of the present invention as defined by the appended patent application scope, and Other aspects.

【Biological Material Storage】 Overseas hosting information [Please note in order of hosting country, institution, date, number]

Republic of Korea, Korea Microbiological Preservation Center, October 09, 2008, KCTC11403BP.

Claims (2)

An antibacterial cosmetic composition for skin, comprising a culture of Lactobacillus germ L. CJLP133 deposited under the accession number KCTC 11403BP, wherein the bacterial body of the Lactobacillus germ L. CJLP133 is removed, wherein the culture is directed to At least one bacterium in the group has antibacterial activity: Staphylococcus epidermidis, E. coli O157: H7 type and Propionibacterium acnes. The cosmetic composition as described in item 1 of the patent application scope, wherein the culture is obtained by a method including the following: (a) cultivating the Lactobacillus germs CJLP133 strain by inoculating a medium; (b) replacing (a) The product obtained in the step is centrifuged; and (c) the product obtained in the step (b) is recovered in the upper part.

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