KR20190050361A - Composition for improving microbial flora containing extract of Allii Fistulosi bulbus - Google Patents

Abstract

Disclosed is a composition for improving microbial flora, comprising an extract of Allii Fistulosi bulbus as an effective component, wherein the composition may suppress the growth of harmful bacteria while promoting the growth of beneficial bacteria. The extract of Allii Fistulosi bulbusis is extracted using a solvent selected from the group consisting of water, an alcohol having 1-4 carbon atoms, ethyl acetate, diethyl ether, dichloromethane, acetone, and a mixture thereof.

Description

Technical Field [0001] The present invention relates to a composition for improving microflora containing an extract of a mung bean as an active ingredient,

The present invention relates to a composition for improving microflora containing a total extract as an active ingredient.

The human body is an ecosystem that is complex and life-active, and various microorganisms exist in the stomach and skin of the human body. Stomach, and skin are referred to as microbial fungi, and 500 to 1000 different microorganisms are symbiotic to form microbial fungi. Intestinal microorganisms present in the stomach synthesize substances such as vitamins and supply them to the human body, and play a role in inhibiting infection by pathogenic microorganisms entering through contaminated food.

Microorganisms that survive on the surface of the skin affect physiological functions such as resistance to human growth, nutrition, immunity and pathogenicity, and play a role in blocking the invasion of external microorganisms, particularly pathogenic microorganisms. In addition, it is known that the microorganisms that reside in the skin perform pH control and skin barrier function of the skin, and change the lipid content of the skin to affect the skin condition. For example, in atopic patients, the ceramide content of the stratum corneum is lowered to have dry and cracked keratin skin. In these atopic patients, it is known that the skin flora is extremely transformed into Staphylococcus aureus (Non-Patent Document One).

Since microorganisms have microorganisms that have a beneficial effect on the human body, it is necessary not to simply remove (sterilize) microorganisms, but to promote the growth of beneficial bacteria while inhibiting the growth of harmful microorganisms.

The present inventors have completed the present invention as a result of carrying out research for improving microorganism microflora using natural products.

 1. HC Korting et al., Eur J Clin Pharmacol 39 (1), 33-36. 1990.

It is an object of the present invention to provide a composition for improving microflora containing a total extract as an active ingredient and a cosmetic composition for skin fungal improvement.

In order to accomplish the above object, one aspect of the present invention provides a composition for improving microflora comprising an extract of Ganoderma lucidum as an active ingredient.

As used herein, the term 'microbial flora' refers to a microbial community comprising all the bacteria, fungi, and other prokaryotes present in the human body, and is inhabited mainly in the stomach and skin. For example, intestinal yuikgyun that live in the stomach has a Lactobacillus bacteria (Lactobacillus), Bifidobacterium (Bifidobacterium), harmful bacteria in the intestines is Clostridium (Clostridium) are known.

As used herein, the term " improvement " refers to a condition in which the composition of microbial fungi changes in a beneficial direction to the human body due to the administration of the composition.

( Allii Fistulosi bulbus) used as an active ingredient of the composition for improving microbial fungus of the present invention refers to a medicament made of fresh scaly stem of Allium fistulosum Linne (Liliaceae), and it is a pharmacological effect, It is known that it excretes, makes good menses, and acts on headache and abdominal pain caused by cold or cold. However, there is no known effect of improving the microflora.

In one embodiment of the present invention, the gum extract may be prepared according to conventional methods known in the art. For example, it is possible to prepare a ganoderma extract by pulverizing a gangue, adding a solvent conventionally used for the extraction, and extracting at an appropriate temperature and pressure.

In one embodiment of the invention, the solvent is selected from the group consisting of water, hexane, 1,3-butylene glycol, alcohols having 1 to 4 carbon atoms, ethyl acetate, diethyl ether, dichloromethane, acetone and mixtures thereof .

In one embodiment of the present invention, the solvent may be an alcohol having 1 to 4 carbon atoms, and methanol or ethanol may be used.

On the other hand, the above-mentioned Gunbagi extract includes both the above-mentioned solvent extraction method and the conventional purification method. For example, separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatographies (made for separation by size, charge, hydrophobicity or affinity) Fractions obtained through various purification methods can also be included in the gum extract of the present invention. The extract can also be prepared in powder form by an additional process such as vacuum distillation and lyophilization or spray drying.

In one embodiment of the present invention, the composition for improving microflora may comprise 0.005 to 10% by weight, or 1 to 5% by weight of the total extract of the composition, based on the total weight of the composition, Accordingly, the content of the active ingredient can be appropriately controlled.

In one embodiment of the present invention, the composition for improving microorganism fungus can promote the growth of beneficial bacteria while inhibiting the growth of harmful microorganisms. Increasing the proportion of the beneficial bacteria in the microorganism can prevent the infection by the pathogenic microorganism, improve the immunity, improve the skin condition, and improve body odor.

In one embodiment of the invention, the harmful bacteria is Propionibacterium sludge tumefaciens (Propionibacterium), Staphylococcus (Staphylococcus), Streptococcus (Streptococcus), Candida (Candida), Acinetobacter (Acinetobacter), Corynebacterium (Corynebacterium ) and be at least one selected from the group consisting of Pseudomonas (Pseudomons), but it is not limited thereto. In addition, the above-mentioned harmful bacteria may be Propionibacterium acnes or Candida albicans .

In one embodiment of the invention, the harmful bacteria can be Staphylococcus aureus (Staphylococcus aureus, Staphylococcus aureus). Staphylococcus aureus is a gram-positive, tuberous anaerobic bacterium that is generally present in the skin and nasal surface of healthy persons or livestock. S. aureus is known to cause not only food poisoning but also purulent diseases such as skin pustules, ear infections and cystitis.

In one embodiment of the present invention, the beneficial bacteria may be Staphylococcus epidermidis ( S. epidermidis ). Staphylococcus epidermidis is a gram-positive bacterium generally present in the skin and mucous membranes of humans and livestock. Staphylococcus epidermidis produces glycerin to sustain the moisturizing effect of the skin (J. Invest. Dermatol., 1960, 34: 171-174), synthesizes organic acids to keep the skin acidic (Br. J. Dermatol 1986, 80: 279-281), it is known to produce antibiotics and inhibit skin growth of harmful microorganisms such as Staphylococcus aureus (N. Engl. J. Med., 1998, 339: 520- 2010, 465: 346-349; Peptides, 2010, 31: 1661-1668; J. Invest. Dermatol., 2010, 130: 192-200).

Another aspect of the present invention provides a cosmetic composition for skin fungal improvement comprising an extract of Ganoderma lucidum as an active ingredient.

As used herein, the term 'skin resident flora' refers to a microbial community present in the skin, and it is mainly found on skin surface and pores such as scalp. The skin fungus includes skin benefit bacteria that exhibit a beneficial effect on the skin such as moisturizing the skin, removing active oxygen, and skin harmful bacteria that cause skin troubles such as acne and acne.

In one embodiment of the present invention, the cosmetic composition may be in the form of a gel, an emulsion, a suspension, a microemulsion, a microcapsule, a microgranule, an ionic follicle dispersant, a nonionic type follicle dispersant, a cream, a skin, And may be formulated in the form of ointments, sprays, conical sticks, aerosols, mask packs, body powders, bodywashes, shampoos, hair conditioners, body lotions, body lotions for deodorants and body mists. Examples of the cosmetic composition include basic cosmetics, makeup cosmetics, hair cosmetics, body cosmetics, and the like, and can be appropriately selected according to the purpose.

For example, the cosmetic composition can be formulated into a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, But is not limited thereto. More specifically, the present invention relates to a cosmetic composition, which comprises at least one of a softening agent, a nutritional lotion, a lotion, a body lotion, a nutritional cream, a massage cream, a moisturizing cream, a hand cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, shampoos, rinses, body cleansers, and cosmetics such as cosmetics, oil-in-water, water-in-oil and w / o type lipsticks, A cleaning agent such as a toothpaste or an oral cleanser, a hair fixative such as a hair tonic, a gel or a mousse, or a hair cosmetic composition such as a hair or hair dye.

In one embodiment of the present invention, when the formulation of the cosmetic composition is a solution or emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol , Benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In one embodiment of the present invention, when the formulation of the cosmetic composition is in the form of a suspension, it is preferable to use, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sor Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, and the like may be used.

In one embodiment of the present invention, when the formulation of the cosmetic composition is a paste, a cream or a gel, the carrier component may be an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracant, a cellulose derivative, polyethylene glycol, silicone, bentonite, silica , Talc or zinc oxide may be used.

In one embodiment of the present invention, when the formulation of the cosmetic composition is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, Can additionally include propellants such as chlorofluorohydrocarbons, propane / butane or dimethyl ether.

In addition, in the cosmetic composition of each formulation, other components other than the above-mentioned extract can be arbitrarily selected and formulated according to the formulation or use purpose of the cosmetic composition. In addition, the compositions of each formulation may contain various bases and additives necessary for formulation of the formulation and may contain various additives such as a nonionic surfactant, a silicone polymer, an extender pigment, a perfume, a preservative Emulsifiers, silicones, alpha-hydroxyesters (such as alpha-hydroxyesters), antioxidants, antioxidants, antioxidants, antioxidants, stabilizers, emollients, a preservative, a surfactant, a filler, a polymer, a propellant, a basicizing agent, an acidifying agent, or a coloring agent.

The extracts of Phellinus linteus inhibit the growth of harmful microorganisms and promote the growth of beneficial bacteria, and thus can be usefully used for the improvement of microflora and the improvement of skin microflora.

FIG. 1 shows the results of measurement of the growth rate changes of Staphylococcus epidermidis and Staphylococcus aureus by treatment with the extract of Ganoderma lucidum.
FIG. 2 shows the growth changes by treatment with 95% ethanol extract of total grains when Staphylococcus aureus and Staphylococcus epidermidis were mixed and cultured.

Hereinafter, one or more embodiments will be described in more detail by way of examples. However, these embodiments are intended to illustrate one or more embodiments, and the scope of the present invention is not limited to these embodiments.

Example 1: Preparation of gum extract

To 5 g total, 95% ethanol or 50 ml methanol (drug: solvent ratio 1:10) was added and shaken in a constant temperature water bath for 3 hours to obtain a primary extract. The primary extract was filtered through a filter paper, and the filtered extract was concentrated and dried to prepare a total extract. 50 mg of the dried extract was dissolved in 1 ml of each extraction solvent, and the solids that did not dissolve in the solvent were removed by centrifugation and only the supernatant was used as the sample.

Example 2: Changes in the microbial growth of skin caused by extract

2-1. Microorganisms and culture medium

The skin harmful bacteria Staphylococcus aureus; and using the (Staphylococcus aureus, Staphylococcus aureus, hereinafter abbreviated as S. aureus), the skin yuikgyun is Staphylococcus epidermidis (Staphylococcus epidermidis, Staphylococcus epidermidis; or less S. epidermidis ) was used.

S. aureus KCTC 1916 and S. epidermidis KCTC 1917 were purchased from the BRC (Korea Collection for Type Cultures, Korea). The composition of the medium used for culturing the two microorganisms is shown in Table 1 below.

S. epidermidis
KCTC 1917 NB badge
(100 ml) Nutrient Broth 0.8 g (Agar) 1.5 g water Replenish to a total volume of 100 ml S. aureus
KCTC 1916 TSB badge
(100 ml) Tryptic Soy Broth 3.0 g (Agar) 1.5 g water Replenish to a total volume of 100 ml

2-2. Changes in Growth of Individual Skin-Residing Microorganisms by Extract

S. aureus strain KCTC 1916 strain was plated on TSB (Tryptic Soy Broth, Becton Dickinson and Company, USA) agar plates and cultured at 37 ° C for 24 hours. Thereafter, a single colony was taken and inoculated into 5 ml of TSB liquid medium and preincubated overnight at 37 < 0 > C and 250 rpm. The pre-cultured S. aureus KCTC 1916 strain was inoculated into 20 ml of TSB liquid medium at an OD ₆₀₀ of 0.05 and 200 μl of a total methanol extract (1% (v / v)) was added. The control group was supplemented with methanol at a concentration of 1% (v / v) instead of the total extract. After incubation at 37 ° C and 250 rpm, the absorbance was measured at 600 nm using an Optizen 2120 UV plus spectrophotometer (Mecasys Co., Ltd., Daejeon, Korea) every hour.

S. epidermidis KCTC 1917 Growth was also measured by culturing the stock in the same manner as described above except that the stock was cultured on NB (nutrients broth, Becton Dickinson and Company) agar plates.

As a result of the measurement, it was confirmed that the growth rate of S. epidermidis was increased and the growth rate of S. aureus was decreased by the methanol extract of the whole plant as shown in FIG. Specifically, the doubling time of S. epidermidis was reduced by about 33.31% in the total extract treatment group (51.50 min) compared to the control group (77.22 min). On the other hand, the doubling time of S. aureus was increased by 5.07% in the control group (35.12 min) compared to the control group (36.9 min).

2-3. Growth changes of skin-resident microorganisms caused by ethanol extracts of 95%

The microorganisms were preincubated in the same manner as in Example 2-2, and S. aureus KCTC 1916 strain and S. epidermidis KCTC 1917 strain pre-cultured were mixedly inoculated into 20 ml of NB liquid medium to have an OD ₆₀₀ of 0.05. 200 μl (1% (v / v)) of 95% ethanol extract (0.5 g / L) was added to the mixture and ethanol was added thereto at a concentration of 1% (v / v) Lt; / RTI > Then, 100 μl of the culture was plated on NB agar plates and cultured at 37 ° C for 24 hours to confirm CFU (colony forming unit).

As a result, as shown in FIG. 2, the growth of S. aureus was remarkably decreased by the treatment with 95% ethanol extract, and it was confirmed that the growth of S. epidermidis was maintained constantly.

It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Therefore, the disclosed embodiments should be considered in an illustrative rather than a restrictive sense. The scope of the present invention is defined by the appended claims rather than by the foregoing description, and all differences within the scope of equivalents thereof should be construed as being included in the present invention.

Claims (5)

Wherein the growth of the harmful microorganisms containing the extract as a active ingredient is inhibited and the growth of the beneficial microorganism is promoted.
2. The composition of claim 1 wherein the extract is extracted with a solvent selected from the group consisting of water, alcohols having from 1 to 4 carbon atoms, ethyl acetate, diethyl ether, dichloromethane, acetone, and mixtures thereof.
2. The composition of claim 1, wherein the total extract is comprised between 0.005 and 10% by weight based on the total weight of the composition.
The composition for improving microflora according to claim 1, which is used in cosmetics.
The composition according to claim 1, wherein the composition for improving microflora is used for a cleaning agent.

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