KR101307969B1 - Cosmetic composition with the fermented mixture of fruits and dark vinegar - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising a fermentation broth obtained by fermenting a mixed solution prepared by mixing fruit (plum, citron, pomegranate) and black vinegar, and a cosmetic composition having excellent skin exfoliation effect, skin regeneration effect and whitening effect. Can be prepared.

Description

Cosmetic composition containing a mixed fermentation broth of fruit and black vinegar {Cosmetic composition with the fermented mixture of fruits and dark vinegar}

The present invention relates to a cosmetic composition, and more particularly to a cosmetic composition comprising a fermentation broth obtained by fermenting a mixture prepared by mixing fruit (plum, citron, pomegranate) and black vinegar.

In the human body, the skin acts as a protective film that protects the living body from the external environment, and prevents the loss of biological components such as water or electrolytes and prevents harmful substances from entering the body. do.

The skin is divided into the skin layer, the dermal layer and the subcutaneous fat layer. The epidermal layer is composed of keratinocytes and melanocytes. Since the outermost keratinocyte layers of skin are in direct contact with the external environment, they require strong resistance to physical, chemical, or permeation of substances, and prevent the loss of moisture from the body to the outside. At the same time, it should maintain flexibility by retaining adequate moisture on its own.

The stratum corneum is the outermost layer in direct contact with the outside, protecting the skin and body from physical and chemical hazards. As skin cells continue to be produced, existing skin cells gradually rise to the stratum corneum, and the cells that reach the stratum corneum are flat, dead cells that cause millions of dead keratinocytes to fall off the skin and be replaced by new ones. Happens. The original skin naturally forms a turnover cycle of spontaneous exfoliation and new skin coming up, but it does not remain natural as it ages or depending on the surrounding environment such as dryness, UV rays and stress. Therefore, the artificial method called peeling removes dead skin cells and restores the regeneration cycle of the blocked skin and raises fresh cells generated from the bottom to keep the skin clear and clean.

Cosmetics for exfoliation include chemical products using acid components such as AHA (alpha hydroxy acid) and BHA (beta hydroxy acid), enzyme products using proteolytic enzymes, and physical products using exfoliation. There is this.

Until recently, most of the chemical exfoliants used in cosmetics are AHA (alpha hydroxy acid) and BHA (beta hydroxy acid), which are glycolic acid, lactic acid, malic acid, citric acid, tartaric acid, salicylic acid, etc. Excellent skin penetration and exfoliating ability.

However, these chemical exfoliants can show excellent efficacy in a short period of time, but have the disadvantage that skin irritation is likely to occur and the effect does not last long. These products are being used gradually because of skin irritation and weakening of the skin surface during repeated use.

On the other hand, Prunus mume Sieb. Et Zucc. (Japanese apricot) is an orchard of rosaceae, and its origin is mainly grown in Japan, Taiwan, China, and Korea from central to southern China. Fruits contain 4 to 5% of acid and are highly acidic, so most of them are used for processing. Varieties include small, neutral or alleles. The main components of the fruit are citric acid and malic acid, with less than 1% sugar. The nucleus of plum contains a lot of amidarin to produce cyanate by enzymatic decomposition. However, the amount of cyanic acid produced is not traceable to food toxicity. 85% of the flesh is water, 10% is rich in sugar, minerals and organic acids and contains carotene. Alkaline food is good for fatigue recovery and constitution improvement. It has excellent detoxification and helps with stomach upset and food poisoning. It is also known as an anticancer food recently.

Citrus junos Tanaka (Chinese lemon) is native to the upper Yangtze River of China, cold resistance is the strongest among citrus fruits, and its distribution is wide. In Korea, it is grown in the southern coastal zone and part of the west coast. Fruits are 100 ~ 130g, monocytes, thick skin, fruit surface is yellow, diffusion is deep, irregularities are severe. Fruits have a characteristic scent, and the flesh is light, succulent, and has a high acid content. It is processed not only with vinegar but also with citron and citron. Yuzu's distinctive scent is based on the amount of essential oil, and its main ingredients are 30-40 species such as limonene, β-pinene, linalool, and citronellol. It is known as a component of. More than 150mg of vitamin C per 100g, more than three times the lemon or navel. Yuzu has been used to treat colds, neuralgia, wind, nausea, vomiting, indigestion, and poisoning. It is also effective in preventing cancer. It also contains hesperidin, which has the same effect as vitamin P, which protects the capillaries and also has a high content of carotene, the vitamin B complex or vitamin A's parent.

Pomegranate (Punica granatum L.) has a tree height of 10m and the stem is twisted. Originated in Iran and Afghanistan, it is estimated to have been introduced in Korea about 500 years ago. Due to the cold, it is unable to grow economically in the central region, and wintering is possible only in the regions of Jeollabuk-do and Gyeongsangbuk-do. Flowers are bisexual and bloom in June in red. Fruits are round and 6-8 in diameter, ripening yellow or yellowish red in September-October. The inside contains red seeds. When fully cooked, the outer shell becomes irregular and the seeds inside are visible. Seeds are delicious and sweet, eat as they are, or make a flower. In addition, the skin has the effects of astringent, hemostasis, branch, and insect repellent. In oriental medicine, Gusari, hematopoiesis, lobular, bubo, dehanging, alluvial It is used for abdominal pain, roundworm, etc. The main ingredient of pomegranate is about 40% of sugar (glucose, fructose), and organic acid contains about 1.5% of citric acid with sour taste, and water-soluble vitamin (B ₁ , B ₂ , niacin), but the amount is small, the skin is tannin, the seeds are known to contain natural phytoestrogens, good for menopausal disorders.

Black vinegar is a vinegar made by fermenting brown rice for more than a year. It is made in Jinjiang, southern China, and has been produced in Japan as a Chinese trader handed it to Japan. After a long fermentation and ripening period, dark vinegar is produced. The sour taste is weak and has a moderate sweetness and aroma. Black vinegar contains more amino acids than other vinegars, and this amino acid is known to be effective in the diet to help prevent blood sugar from turning into fat and help blood sugar to be consumed in muscles.

Korean Patent Laid-Open Publication No. 10-2010-0111640 (published date: October 15, 2010) relates to a method for preparing a cosmetic composition for promoting hair loss prevention, anti-inflammatory action, hair root cell activation and hair growth, stress in vivo It shows excellent hair loss prevention and hair growth promoting effect against hair loss and hair growth inhibition caused by inflammatory expression.It also has less skin irritation, protects scalp by relieving inflammation and stress, and treats baldness and alopecia areata. A composition comprising black vinegar extract as an active ingredient, which can be usefully used as an anti-hair loss agent, a scalp protector, and a hair growth promoting agent, is described.

The present invention is applicable to skin cosmetics using plum, citron, pomegranate and black vinegar, harmless to the human body to develop and provide a cosmetic composition excellent in safety.

The present invention comprises the steps of preparing a mixture by mixing plum, citron, pomegranate with black vinegar; In the mixed solution of fermenting microorganism Lactobacillus bacteria (Lactobacillus) or acetonitrile spp bakteo (Acetobacter) It provides a cosmetic composition comprising a fermented product prepared from the process comprising; and adding the genus strain to fermentation.

In the present invention, plum, citron, pomegranate, black vinegar was selected based on the results of the study of the applicability to cosmetics, the fermentation broth fermented a mixture of plum, citron, pomegranate and black vinegar using the fermentation microorganism of the present invention is conventional plum , Yuzu, pomegranate and black vinegar or each of them fermented skin exfoliation effect, skin regeneration effect and whitening effect was superior.

In addition, the fermentation broth of the present invention is compared with non-fermented fruits (plum, citron, pomegranate) or fermented products, respectively, citric acid, lactic acid, lactic acid, malic acid, pyruvic acid, and pyruvic acid. It is very rich in tartaric acid, etc., so it has excellent skin exfoliation effect, skin regeneration effect and whitening effect.

In addition, in the present invention, after mixing the plum, citron, pomegranate with black vinegar to prepare a mixed solution, fermented microorganisms were inoculated and fermented, the organic acid was richer than when not fermented, and skin irritation was also low. It also showed better exfoliation, whitening and cell regeneration.

On the other hand, in the cosmetic composition of the present invention. The plum, citron, pomegranate is preferably used in the form of fruit juice pulverized each juice. When used in the form of fruit juice, because fermentation can proceed quickly and easily. In the present invention, the content of citron juice is preferably 1-50% by weight, the content of plum juice is 1-50% by weight, the content of pomegranate juice is 1-50% by weight, and the content of black vinegar is 1-50% by weight. Can be added and mixed.

On the other hand, in the cosmetic composition of the present invention. The fermented product may be a powder obtained by drying the fermentation broth after liquid phase or after fermentation. If necessary, the fermented product may be purified through a process such as filtration.

On the other hand, in the cosmetic composition of the present invention. The strain of the genus Lactobacillus is Lactobacillus as an example ( Lactobacillus) acidophilus ) , and the acetobacter genus strain may be, for example, acetobacter aceti .

On the other hand, in the cosmetic composition of the present invention. The fermentation is acetobacter strain or Lactobacillus strain when both strains, 10 ⁵ ~ 10 ⁹ cfu / ml of the two bacteria at a concentration of 1: 9 ~ 9: 1 inoculated in the mixed solution, 15 ~ It can be fermented at 35 ° C. for 1-10 days.

On the other hand, in the cosmetic composition of the present invention. The cosmetic composition preferably contains 0.001 to 30.0% by weight of the fermented product on a dry weight basis. It is because when the fermentation broth is contained within the above range, the effect of the fermentation broth is sufficiently exhibited without compromising the quality aptitude of the cosmetic composition.

On the other hand, the cosmetic composition according to the present invention may contain, in addition to the fermentation broth of the present invention, other ingredients that can give a synergistic effect to the main effect within the range of not impairing the main effect of the present invention. The compositions according to the invention contain auxiliaries commonly used in the cosmetic field, such as hydrophilic or lipophilic gelling agents, hydrophilic or lipophilic active agents, preservatives, antioxidants, solvents, fragrances, fillers, blockers, pigments, odorants and dyes. can do. The amounts of these various adjuvants are amounts conventionally used in the art, such as 0.001-30% by weight relative to the total weight of the composition. However, in any case, the adjuvant and its proportion will be selected so as not to adversely affect the desirable properties of the cosmetic composition according to the present invention.

On the other hand, in the cosmetic composition of the present invention. The cosmetic composition may be preferably for exfoliating skin, regenerating skin cells or for skin whitening.

On the other hand, the formulation of the cosmetic composition of the present invention, preferably a lotion, gel, water-soluble liquid, cream, essence, oil-in-water (O / W) and water-in-oil (W / O) type emulsion, ointment cosmetic formulation, The oil-in-water type and the water-in-oil makeup base, foundation, skin cover, lipstick, lip gloss, face powder, two-way cake, eye shadow, cheek color and eyebrow pencils are selected from any one of the color cosmetic formulation. It may also optionally be applied to the skin in aerosol form, in solid form, e.g. in the form of a stick, and as a skin care and / or makeup product.

According to the present invention, it is possible to produce a cosmetic fermentation broth with excellent skin exfoliation effect, skin regeneration effect and whitening effect.

Fermentation broth of the present invention can be applied as a skin cosmetic, it can be an excellent cosmetic raw material harmless to the human body.

1 is a graph of HPLC analysis results showing the organic acid content of the citron, plum, pomegranate, black vinegar before and after fermentation and the total organic acid content of the fermentation broth of Preparation Example 3 and the mixed solution of Comparative Example 1 according to the present invention.
2 is a graph of HPLC analysis results of total organic acid contents for Preparation Examples 1 and 2 and Comparative Example 1. FIG.
3 is a graph of HPLC analysis results of various organic acid contents for Preparation Examples 1 and 2 and Comparative Example 1. FIG.

Hereinafter, the contents of the present invention will be described in more detail through the following Preparation Examples and Examples. However, the scope of the present invention is not limited to the following Preparation Examples and Examples, but includes modifications of equivalent technical ideas.

Manufacturing example  1 to 3: fruits and Black vinegar  Fermentation broth preparation of mixed liquor

Citron, plum and pomegranate were screened with fruit samples, sufficiently washed and ground to make fruit juice. Subsequently, the ratio of citron juice: plum juice: pomegranate juice: black vinegar was added at a ratio of 40: 30: 10: 20 to prepare a mixed liquid.

As Preparation Example 1 according to the present invention, Acetobacter in the finished mixed solution aceti (KTCT 1010) and Lactobacillus acidophilus (KTCT 3140) was inoculated at a ratio of 1: 9 at a concentration of 10 ⁵ to 10 ⁷ cfu / ml and incubated at room temperature for 7 days. After the culture, the culture solution was centrifuged to first remove the culture, and the filtrate thus obtained was finally filtered using a filter of 0.25 uM (Preparation Example 1).

On the other hand, as in Preparation Example 2 according to the invention, and Acetobacter aceti (KTCT 1010) for the finished mixture of the Lactobacillus the acidophilus (KTCT 3140) with 10 ⁵ ~ 10 ⁷ cfu / ml concentration of 9: inoculated at a ratio of 1: 1 and incubated for 7 days at room temperature. After the culture, the culture solution was centrifuged to first remove the culture medium, and the filtrate thus obtained was finally filtered using a filter of 0.25 uM (Preparation Example 2).

On the other hand, as Preparation Example 3 according to the present invention, Acetobacter in the mixed fruit and black vinegar solution aceti (KTCT 1010) and Lactobacillus acidophilus (KTCT 3140) was inoculated at a ratio of 5: 5 at a concentration of 10 ⁵ to 10 ⁷ cfu / ml and incubated at room temperature for 7 days. After the culture, the culture solution was centrifuged to remove the culture cells first, and the filtrate thus obtained was finally filtered using a filter of 0.25 uM (Preparation Example 3).

Comparative example  One: Non-fermentation  Fruit and Black vinegar  Mixed solution manufacturing

Except for fermentation using a fermentation microorganism, a mixture of fruit juice and black vinegar was obtained in the same manner as in Preparation Example (Comparative Example 1).

Test Example  1: Comparison of Organic Acid Content

In this test example, the total organic acid content and various organic acid contents of the fermentation broths of Preparation Examples 1 to 3 and Comparative Example 1 were analyzed by HPLC.

1 is a graph of HPLC analysis results showing the organic acid content of the citron, plum, pomegranate, black vinegar before and after fermentation and the total organic acid content of the fermentation broth of Preparation Example 3 and the mixed solution of Comparative Example 1 according to the present invention.

2 is a graph of HPLC analysis results of total organic acid contents for Preparation Examples 1 and 2 and Comparative Example 1. FIG.

3 is a graph of HPLC analysis results of various organic acid contents for Preparation Examples 1 and 2 and Comparative Example 1. FIG.

As shown in Figures 1 to 3, when comparing the total organic acid and the respective organic acid content of Preparation Examples 1 to 3 and Comparative Example 1, after the fermentation was carried out than before the fermentation, more organic acids were produced, in particular Acetobacter In Preparation Example 2, where the amount of aceti strain was higher, more organic acid was found.

Test Example  2: skin irritation experiment

The degree of irritation on the skin of rabbits was measured for Preparation Examples 1, 2 and Comparative Example 1, and for this purpose, hair removal of rabbits was performed 24 hours before application of the composition to treat treatment of normal skin and wound skin. And control compartments. The coating method was applied once to the application site by 1 gram per rabbit. Untreated control compartments were applied in equal amounts of sterile physiological saline and covered with gauze. In order to prevent evaporation of the sample, it was covered with a solid material foil having no permeability and reactivity, and fixed with a tape and then applied for 24 hours, 72 hours, and 120 hours. After the end of the application time using a saline solution was lightly cleaned with the application site. The appearance, feed and beverage consumption and clinical symptoms were observed daily for one week after the administration of the sample material.

Evaluation of skin reaction was judged using 'toxicity test criteria' of medicines. In addition, the degree of irritation to the skin was evaluated by the method of calculating P.I.I. (Primary Irritation Index) of Draize which is commonly used, and the results are shown in Table 1 below.

Name of sample P.I.I. Results 24 hours 72 hours 120 hours Control group 0.1 (no irritation) 0.1 (no irritation) 0.1 (no irritation) Production Example 1 0.8 (low stimulation) 0.3 (low stimulation) 0.1 (no irritation) Production Example 2 0.5 (low stimulation) 0.2 (low stimulation) 0.1 (no irritation) Comparative Example 1 1.0 (low stimulation) 0.5 (low stimulation) 0.3 (low stimulation) P.I.I. (No stimulation) 0 5 (strong stimulation)

As shown in Table 1, PII is 0.8 ~ 0.5 hypoallergenic when applied for 24 hours, 0.5 ~ 0.1 when applying 72 hours, non-irritating to 0.1 when applying 120 hours, the composition of the present invention The skin irritation was found to be small.

Example  1, 2, and Comparative example  2, 3: Cosmetics  Produce

In Examples 1 and 2 and Comparative Examples 2 and 3, cosmetics were prepared using the compositions of Preparation Examples 1 and 2 and Comparative Example 1. The prepared cosmetic was in the form of a cream, the composition was as shown in Table 2.

Unit: wt% Raw material Example 1 Example 2 Comparative Example 2 Comparative Example 3 end





Stearyl alcohol 8 8 8 8 Stearic acid 2 2 2 2 Stearic Acid Cholesterol 2 2 2 2 Squalane 4 4 4 4 2-octyldodecyl alcohol 6 6 6 6 Polyoxyethylene (25 mol addition) alcohol ester 3 3 3 3 Glyceryl Monostearic Acid Ester 2 2 2 2 I



Production Example 1 5 - - - Production Example 2 - 5 - - Comparative Example 1 - - 5 - Propylene glycol 5 5 5 5 Purified water Balance Balance Balance Balance

The 'Na' phase recorded in Table 2 was heated and stored at 70 ° C. After preserving emulsification by adding the 'ga' phase to the preserved 'na' phase, the emulsion was uniformly emulsified with a homomixer and then gradually cooled. Creams (Examples 1 and 2 and Comparative Examples 2 and 3) were prepared.

Test Example  3: exfoliation and whitening effect experiment

Examples 1 and 2 and Comparative Examples 2 and 3 were applied to the skin, and after 1 week, skin exfoliation and whitening effects were measured.

Thirty women aged 20 to 50 were randomly divided into three groups. Group 1 took Comparative Example 3 on the left side of the face and Example 1 on the right side of the face, massaged for 10 minutes and washed for 7 days. Was carried out. Group 2 was carried out for 7 days by the method of taking Comparative Example 3 on the left side of the face and Example 2 on the right side of the face, massaging and washing face for 10 minutes. Similarly, Group 3 was carried out for 7 days by the method of taking Comparative Example 3 on the left side of the face and Example 2 on the right side of the face, massaging for 10 minutes and washing the face.

The evaluation was divided into 0 to 5 grades according to the presence or absence of the effect, and the larger the number, the greater the effect. The average value was calculated for each effect and the results are shown in Table 3 below.

Name of sample Group 1 Group 2 Group 3 Comparative Example 3 Example 1 Comparative Example 3 Example 2 Comparative Example 3 Comparative Example 2 Skin irritation 0.6 1.1 0.8 0.9 0.7 1.7 Exfoliation 1.2 3.7 1.0 4.0 1.2 2.6 Whitening effect 1.6 3.5 1.5 4.1 1.5 2.8 Efficacy and degree of stimulation (weak) 0 5 (strong)

As shown in Table 3, in all the groups, the exfoliation effect and the whitening effect were not apparent in Comparative Example 2 compared to Comparative Example 3, but in Examples 1 and 2, the exfoliation effect and the whitening effect thereof were increased. I could see.

Test Example  4: cell regeneration effect experiment

Examples 1 and 2 and Comparative Examples 2 and 3 were applied to the skin, and after one week the effect of cell regeneration was measured.

In the test subject of Test Example 3, the melanin index on both sides of the face was measured by melanin-erydemma index meter to measure the effect of cell regeneration. Table 4 shows the results of before and after the use of Examples 1 and 2 and Comparative Examples 2 and 3.

Name of sample Melanin index Before use after use Example 1 112.35 93.46 Example 2 110.52 91.58 Comparative Example 2 110.84 102.69 Comparative Example 3 109.76 105.73

As shown in Table 4, when the cosmetics of Examples 1 and 2 were used, it was found that the melanin index was reduced more than that of Comparative Examples 2 and 3, and effectively exfoliated in Examples 1 and 2 according to the present invention. It was found that the removal may have a skin color improvement effect by skin cell regeneration.

Claims (9)

Preparing a mixed liquid by mixing plum, citron and pomegranate with black vinegar;
In the mixed solution of fermenting microorganism Lactobacillus bacteria (Lactobacillus) or acetonitrile spp bakteo (Acetobacter) Adding a fermentation strain of the genus; Cosmetic composition characterized in that it comprises a fermentation product prepared from the process comprising a.
The method of claim 1,
The plum, citron, pomegranate,
Cosmetic composition, characterized in that each is in the form of fruit juice pulverized juice. The method of claim 1,
The fermented product may contain,
Cosmetic composition, characterized in that the powder obtained by drying the fermentation broth after liquid or fermentation.
The method of claim 1,
The strain Lactobacillus,
Lactobacillus Lactobacillus acidophilus ) ,
Said acetobacter strain,
Acetobacter aceti ( Acetobacter aceti ) Cosmetic composition, characterized in that.
The method of claim 1,
The above-
When both acetobacter strains or Lactobacillus strains are used, the two strains at a concentration of 10 ⁵ to 10 ⁹ cfu / ml are inoculated into the mixed solution at a ratio of 1: 9 to 9: 1, and then, at 15 to 35 ° C. Cosmetic composition, characterized in that fermentation for 1 to 10 days.
The method of claim 1,
In the cosmetic composition,
Cosmetic composition characterized in that it contains 0.001 to 30.0% by weight of the fermented product on a dry weight basis.
The method of claim 1,
In the cosmetic composition,
Cosmetic composition, characterized in that for removing the skin exfoliation.
The method of claim 1,
In the cosmetic composition,
Cosmetic composition for skin cell regeneration.
The method of claim 1,
In the cosmetic composition,
Cosmetic composition, characterized in that for skin whitening.

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