KR101730852B1 - Cosmetic composition comprising fermented color food extracts - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a color food fermentation extract. According to the present invention, there is provided a cosmetic composition comprising a combination fermentation extract of pomegranate, tomato, broccoli, fortified wormwood, turmeric, soybean, garlic, ginseng, / RTI >

Description

[0001] The present invention relates to a cosmetic composition comprising fermented color food extracts,

The present invention relates to a cosmetic composition containing a color food fermentation extract, and more particularly, to a cosmetic composition containing a complex fermentation extract obtained by combining natural products having various colors and fermentation extraction.

Color food means a natural food with a unique color that helps to health. It is known that natural pigments contained in fruits and vegetables remove bad oxygen in the body, increase immunity, and prevent various diseases.

The green chlorophyll contained in the green food smoothes metabolism, relieves fatigue, helps regenerate cells, and helps prevent aging. Catechin, the main component of green tea, has antioxidant and anti-cancer effects, and it has excellent ability to remove cholesterol from the body and eliminate odor. Plum (梅 实) contains citric acid, malic acid, and organic acids such as succinic acid is strong sour taste, poison (poison) of the food, the blood poison is effective to eliminate. The sulforaphane component of borocoli has anticancer effect, and broccoli contains beta carotene, vitamin C, E and selenium which have antioxidant effect.

The red colored lycopene contained in the red food has anti-cancer effect, increased immunity, strengthened blood vessels, and anthocyanin has antioxidant activity. Tomato lycopene is excellent in anticancer activity, contains various minerals and vitamins, and low calorie food. The polyphenol component of red wine is effective in preventing heart disease. Capsaicin, a volatile component of hot peppers, has anti-cancer, antioxidant and appetite-promoting effects.

Black anthocyanins contained in black foods help to increase antioxidant ability and increase immunity and prevent aging. Black rice (black rice) contains a large amount of calcium, phosphorus, iron and vitamin B group. Black beans are abundant in protein, fiber, calcium, iron and zinc to promote brain activity, prevent osteoporosis, regulate hormone secretion, prevent aging, have strong anticancer and antioxidant activity. Sesamol, sesaminol, and tocopherol in black sesame seeds enhance the body's overall function and are enriched with essential amino acids, essential fatty acids, calcium and iron.

The yellow carotenoids contained in yellow foods have anticancer, antioxidant and anti-aging effects. Amber is rich in vitamins A, B, C and potassium, and excretes waste and diuresis. Sweet potato contains a lot of vitamin B, C, tocopherol and fiber.

The white flavonoid component inhibits the anticancer effect and the body's oxidizing action, releasing harmful substances out of the body, and enhancing resistance against bacteria and viruses. Alicin in garlic has anti-cancer effect and cholesterol-lowering effect. Sulfur compound and quercetin, which are the tear components of onion, have anticancer and antioxidant activity. Onions also contain vitamin C, selenium and dietary fiber to prevent aging and cancer. Potatoes are rich in pantothenic acid, vitamin C and potassium.

So far, the development of cosmetics using natural products has been actively carried out. Korean Patent Publication No. 2012-0007170 "Method of fermenting mackerel or extract thereof, fermented extract thereof and cosmetic composition containing the same ", the term " mackerel or extract thereof is naturally fermented, or fermented by adding a fermentation strain or a certain amount of salt And extracts it with a solvent to prevent the propagation of germs such as spoilage bacteria, Escherichia coli and anaerobic bacteria which are harmful to human body and to eliminate odor and the like, and to enhance the whitening, anti-aging, anti wrinkle, Fermentation method, fermented extract thereof and cosmetic composition containing the same ", Korean Patent Laid-Open Publication No. 2011-0089580 entitled" Cosmetic Composition Containing Vitamin Tree Fermented Extract ", " Vitamin tree, leaf, Saccharomyces cerevisiae, Aspergillus oryzae, Bacillus subtilis or Lactobacillus bulg The present invention relates to a safe cosmetic composition having an excellent effect in improving skin healing, moisturizing, whitening and anti-aging by using an extract obtained by fermenting or fermenting natural fermented soybeans or aricus.

The present inventors have conducted studies to use various color foods among natural products as cosmetics and selected pomegranate, tomato, broccoli, fortified wormwood, turmeric, soybean, garlic, ginseng, black rice and black bean among a variety of color foods, As a result of the extraction, it was confirmed that the complex fermented extract exhibits excellent skin whitening, skin regeneration, wrinkle improvement, and skin soothing effects.

It is an object of the present invention to provide a cosmetic composition containing a complex fermentation extract of pomegranate, tomato, broccoli, fortified wormwood, turmeric, soybean, garlic, ginseng, black rice and black soybean.

It is another object of the present invention to provide a cosmetic composition which contains the complex fermentation extract and exhibits skin whitening effect, skin regeneration effect, wrinkle improving effect, skin soothing effect, skin aging prevention effect, acne improvement and atopy improvement effect do.

It is still another object of the present invention to provide a method for producing a complex fermented extract of pomegranate, tomato, broccoli, fortified warp, cauliflower, soybean, garlic, ginseng, black rice and black soybean.

To achieve the above object, according to the present invention, there is provided a cosmetic composition comprising a complex fermentation extract of pomegranate, tomato, broccoli, fortified wormwood, turmeric, soybean, garlic, ginseng, black rice and black soybean.

The complex fermentation extract is obtained by mixing 1 to 30 parts by weight of pomegranate, tomato, broccoli, fortified wormwood, turmeric, soybean, garlic, ginseng, black rice and black bean respectively.

The complex fermentation extract is contained in an amount of 0.1 to 30% by weight based on the total weight of the cosmetic.

The cosmetic composition exhibits skin whitening effect, skin regeneration effect, wrinkle improvement effect, skin soothing effect, skin aging prevention effect, acne improvement effect and atopy improvement effect.

According to another aspect of the present invention, there is provided a method for producing a poultry meat comprising the steps of: A) mixing 1 to 30 parts by weight of dried and pulverized pomegranate, tomato, broccoli, fortified wormwood, turmeric, soybean, garlic, ;

B) adding a cellulase to the mixture together with 1 to 10 times the weight of the solvent and treating with the enzyme for 12 to 24 hours;

C) fermenting yeast for 48 to 72 hours; And

D) filtration followed by concentration under reduced pressure.

The combined fermentation extract of pomegranate, tomato, broccoli, fortified warp, cauliflower, soybean, garlic, ginseng, black rice and black soybean according to the present invention is effective for skin whitening effect, skin regeneration effect, wrinkle improvement effect, Skin aging prevention effect, acne improvement effect and atopy improvement effect, the cosmetic composition containing the same exhibits various skin improving effects.

Hereinafter, the present invention will be described in detail.

According to the present invention, there is provided a cosmetic composition comprising, as an active ingredient, a complex fermented extract of pomegranate, tomato, broccoli, fortified warp, cauliflower, soybean, garlic, ginseng, black rice and black soybean.

Pomegranates, tomatoes, broccoli, fortified wormwood, turmeric, soybean, garlic, ginseng, black rice and black bean, which are used as raw materials for the complex fermentation extract, contain various natural pigments and useful ingredients for skin. In case of extracting them in combination, it was confirmed that the skin improving effect is enhanced by the synergistic action between the active ingredients. In addition, the present invention combines the above components and applies a fermentation extraction process, whereby the skin permeability is superior to that of a general solvent extract, and the activity is more excellent.

10 to 15 parts by weight of 10 to 15 parts by weight of each of pomegranate, tomato, broccoli, fortified wormwood, turmeric, soybean, garlic, ginseng, black rice and black soybean are preferably mixed in a proportion of 1 to 30 parts by weight, 5 to 10 parts by weight 5 to 10 parts by weight 5 to 10 parts by weight 5 to 10 parts by weight 5 to 10 parts by weight 5 to 10 parts by weight 3 to 5 parts by weight 3 to 5 parts by weight By weight.

According to one embodiment of the present invention, the complex fermentation extract may be prepared by the following method.

First, the dried pomegranate, tomato, broccoli, fortified mugwort, turmeric, soybean, garlic, ginseng, black rice and black bean are respectively mixed at a ratio of 1 to 30 parts by weight. The cellulase is added together with the solvent of 1 to 10 times by weight and the enzyme is treated for 12 to 24 hours. Water is preferably used as the solvent, and the cellulase is added in a proportion of 2 to 4% by weight based on the mixed powder. The fermentation can be performed more easily through the pretreatment.

After enzyme treatment, yeast is added and fermented at room temperature for 48 ~ 72 hours. As the yeast, Saccharomyces sp. Strain can be used. The fermented product is filtered and concentrated under reduced pressure to prepare a color food complex extract. As described above, since water is used as a solvent and fermentation is performed at room temperature, it is not irritating and is easy to manufacture.

The combined fermented extracts of pomegranate, tomato, broccoli, fortified wormwood, turmeric, soybean, garlic, ginseng, black rice and black soybean produced in this manner are effective for skin whitening effect, skin regeneration effect, wrinkle improvement effect, skin soothing effect, , Acne improvement effect and atopy improvement effect were excellent.

Therefore, the cosmetic composition containing the complex fermentation extract can be usefully used as a cosmetic for skin whitening, wrinkle reduction, skin irritation alleviation, acne improvement, and atopic dermatitis.

The complex fermentation extract is preferably contained in an amount of 0.1 to 30% by weight based on the total weight of the cosmetic composition.

The cosmetic composition of the present invention can be manufactured into any of the conventionally prepared formulations and examples thereof include lotion, cream, essence, cleansing foam, cleansing water, pack, body lotion, body oil, body gel, shampoo, Conditioner, hair gel, foundation, lipstick, mascara, make-up base and the like.

[Example]

Hereinafter, the present invention will be described in detail by way of the following examples and test examples, but the present invention is not limited to these examples.

Example 1: Preparation of complex fermentation extract

Dried pomegranate, 15 g of tomato, 10 g of broccoli, 10 g of mugwort, 10 g of turmeric, 10 g of soybean, 10 g of garlic, 10 g of ginseng, 5 g of black rice and 5 g of black soybean were crushed and mixed. 1 g of enzyme (cellulase) and 69 g of purified water were mixed with 30 g of the pulverized composite cereal, and the mixture was subjected to enzyme treatment for 24 hours. After enzymatic treatment, saccharomyces cerevisiae was added and fermented for 72 hours at room temperature.

Test Example 1: Confirmation of skin whitening effect

The tyrosinase activity inhibitory effect of the complex fermentation extract prepared in Example 1 was confirmed. As a comparative example, arbutin known to have skin whitening activity was used.

Tyrosinase is an enzyme that stimulates the oxidation process of tyrosine in vivo to produce melanin. Tyrosinase was isolated and purified from mushrooms and purchased from Sigma. The substrate, L-tyrosine (Sigma), was dissolved in 0.05 M sodium phosphate buffer (pH 6.8) and used as a 0.1 mg / ml solution. The complex fermented extract was dissolved in a 0.05 M sodium phosphate buffer solution (pH 6.8) adjusted to a suitable concentration and then used. 0.5 ml of L-tyrosine solution was put into a test tube, 0.5 ml of the compound fermentation extract sample solution was added thereto, and the mixture was allowed to stand at 37 DEG C for 10 minutes. Thereafter, 0.5 ml of 200 units / ml tyrosinase was added to the sample solution, and the mixture was reacted at 37 占 폚 for 10 minutes. The reaction tube was placed on ice to quench the reaction, and the absorbance at 475 nm was measured using a spectrophotometer. As a control group, 0.5 ml of buffer solution was used instead of the above-mentioned sample.

The inhibition rate of tyrosinase activity of each sample was calculated according to the following equation.

Tyrosinase activity inhibition rate (%) = [(A-B) / A] 100

A: absorbance at 475 nm of the reaction solution to which no sample was added

B: Absorbance at 475 nm of the reaction solution to which the sample was added

The results are shown in Table 1 below.

sample Inhibition rate of tyrosinase activity (%) Control group - Combined fermented extract 0.02 wt% 55 Combined fermentation extract 0.01 wt% 42 Complex fermentation extract 0.005 wt% 38 Arbutin 0.2 wt% 58

As shown in Table 1, the combined fermentation extract of Example 1 exhibited a high inhibition of tyrosinase activity, and the 0.02% combined fermentation extract exhibited a tyrosinase inhibition rate similar to 0.2 wt% of arbutin. From this, it can be seen that the complex fermentation extract of the present invention has excellent skin whitening activity.

Test Example 2: Confirmation of MMP-1 activity inhibitory effect

In order to measure the inhibition rate of MMP-1 activity of the complex fermentation extract obtained in Example 1, green tea extract was used as a comparative sample, and fluorescence analysis was used. The substrate used was DQ-collagen labeled with fluorophore and the collagenase was purchased from Molecular probe (Eugene, OR, USA) and incubated in reaction buffer (0.5 M Tris-HCl, 1.5 M NaCl, 50 mM CaCl ₂ , 2 mM sodium azide, pH 7.6) was used after 10-fold dilution. 20 μl of DQ collagen dissolved in the reaction buffer and 40 μl of the fermented herbal extract (0.02, 0.01, 0.005% by weight) were added to 100 μl of the reaction buffer at 0.25 mg / ml, and 40 μl of collagenase diluted to 0.5 unit was added. After 20 minutes at room temperature, the cow was measured for fluorescence with an absorption wavelength of 495 nm and an emission wavelength of 515 nm using a fluorescence spectrophotometer (Perkin Elmer, UK). In the control group, fluorescence value was measured after addition of the same amount of enzyme buffer as the reaction buffer instead of the enzyme solution, and the fluorescence value of the sample itself was also measured to calibrate the enzyme activity.

Name of sample MMP-1 activity inhibition rate (%) Combined fermented extract 0.02 wt% 91 Combined fermentation extract 0.01 wt% 66 Complex fermentation extract 0.005 wt% 47 Green tea extract 0.2 wt% 67

(MMP-1) activity inhibition rate in vitro (Table 2). The complex fermentation extract effectively inhibited the activity of metalloproteinase (MMP-1) at a lower concentration than that of green tea extract I could confirm.

From the above results, it was confirmed that the complex fermentation extract had an excellent inhibitory effect on metalloproteinase (MMP-1) activity, and based on this, the complex fermentation extract effectively inhibited the metalloproteinase, Wrinkle formation can be prevented.

Test Example 3: Procollagen biosynthesis promotion test

TGF-beta was used as a positive control to confirm the effect of accelerating the biosynthesis of type 1 procollagen, which is a skin substrate component of the complex fermentation extract obtained in Example 1, and a procollagen assay was performed as follows Respectively.

Human dermal fibroblasts isolated from neonatal foreskin tissues were purchased from Modern Tissue Technology (MTT, Korea) and supplemented with 10% fetal bovine serum (FBS) in DMEM / F-12 (3: And cultured at a concentration of 1 × 10 ⁴ cells / cm ² . When 70-80% of the cells were grown, the cells were subcultured at a ratio of 1: 3, and cells in the third to fourth subculture were used for the experiment.

In order to test the amount of procollagen, fibroblasts were cultured in a 48-well plate at a concentration of 90% or more, and the fermented herbal extract was added at concentrations of 0.02%, 0.01% and 0.005% The amount of procollagen was measured using a procollagen type-1 C-peptide EIA kit (MK101, Takara, Japan).

Name of sample Promoting effect of procollagen biosynthesis (%) Combined fermented extract 0.02 wt% 38 Combined fermentation extract 0.01 wt% 18 Complex fermentation extract 0.005 wt% 15 TGF-beta 0.001 wt% 36

As a result of measuring the promoting effect of procollagen biosynthesis (Table 3), when 0.02% of the combined fermentation extract was treated, the biosynthesis of procollagen was promoted by 38%, which was similar to that of the cell signaling substance TGF-β. From the above results, it was confirmed that the complex fermentation extract promoted the excellent procoagulant biosynthesis promotion effect, and the skin improvement effect of the present invention can be deduced.

Formulation Example 1: Preparation of lotion containing complex fermented extract

Lotion containing the complex fermentation extract (Example 1) was prepared according to a conventional method with the composition and content shown in Table 4 below.

Raw material Weight (g) Purified water 82.6 vegetable oil 6.0 glycerin 3.0 Emulsifier 1.0 Spices 0.3 Alcohol 5.0 Complex fermentation extract 2.0

Formulation Example 2: Preparation of lotion containing complex fermentation extract

The lotion containing the complex fermentation extract (Example 1) was prepared according to a conventional method with the composition and content shown in Table 5 below.

Raw material Weight (g) Purified water 84.6 glycerin 6.0 Emulsifier 1.0 vegetable oil 6.0 antiseptic 0.1 Spices 0.3 Complex fermentation extract 2.0

Test Example 4: Confirmation of skin soothing effect

The present invention relates to a cosmetic composition containing a complex fermented extract according to the present invention and a cosmetic composition containing the complex fermented extract according to the present invention, The degree to which stimulation induced by applying a cosmetic composition not contained was calibrated relative to each other. First, taping was repeated 30 times with a scotch tape on both sides of the underbody to cause skin erythema. After erythema induction, the cosmetic composition containing the complex fermentation extract according to the present invention and the cosmetic composition not containing it were applied. The degree of skin soothing was evaluated 3 hours after application.

≪ Evaluation of degree of skin irritation (erythema) >

0 (no response) 1 (very mild erythema) 2 (faint or mild erythema)

3 (clear erythema but weak erythema) 4 (pronounced erythema) 5 (severe erythema)

Name of sample Degree of skin irritation Left Right Formulation Example 1 0 0 Control Example 2 2

As shown in Table 6, the cosmetic lotion of Formulation Example 1 containing the complex fermentation extract of the present invention was superior in the skin irritation mitigation effect as compared with the control without the extract.

Test Example 5: Confirmation of acne improvement effect

Formulation Example 1 containing the complex fermentation extract and the cosmetic preparation of Comparative Example 1 not containing the extract were used to evaluate acne improvement and degree of healing. Twenty subjects of the experiment were subjected to the cosmetic preparation of Comparative Example 1 on the left side and the cosmetic preparation of Formulation Example 1 on the right side to the same person. After two weeks, the improvement of acne and the degree of healing were evaluated. The results are shown in Table 7 below.

division Acne improvement and healing persons % Formulation Example 1 very good 7 35 satisfied 6 30 usually 4 20 Slightly dissatisfied 2 10 dissatisfaction One 5 Control Example 1 very good 0 0 satisfied One 5 usually 7 35 Slightly dissatisfied 7 35 dissatisfaction 5 25

As a result, as shown in Table 7, it was confirmed that the cosmetic composition of Formulation Example 1 containing the composition according to the present invention was used, and that the acne improvement effect was excellent.

Test Example 6: Confirmation of atopy improvement effect

Twenty patients aged 5 to 20 years who had atopic dermatitis were examined for the improvement of atopic dermatitis. In the same person, Formulation Example 1 was applied to the left hand, Control Example 1 was applied to the right hand every evening, and the skin was applied to the skin once a day for 30 days to evaluate the degree of improvement of atopic dermatitis. The results are shown in Table 8 below.

division Atopic dermatitis improvement persons % Formulation Example 1 very good 7 35 satisfied 7 35 usually 4 20 Slightly dissatisfied 2 10 dissatisfaction 0 0 Control Example 1 very good 0 0 satisfied 0 0 usually 4 20 Slightly dissatisfied 8 40 dissatisfaction 8 40

As can be seen from Table 8, it was confirmed that the cosmetic composition containing the complex fermented extract of the present invention exhibits excellent atopic dermatitis improving effect.

Claims (9)

1 to 30 parts by weight of dried and pulverized pomegranate, tomato, broccoli, fortified wormwood, turmeric, soybean, garlic, ginseng, black rice and black bean respectively; Adding the cellulase to the mixture together with 1 to 10 times the weight of the solvent and treating with the enzyme for 12 to 24 hours; Adding yeast (saccharomyces cerevisiae) and fermenting for 48 to 72 hours; And extracting the complex fermentation extract of pomegranate, tomato, broccoli, fortified wormwood, turmeric, soybean, garlic, ginseng, black rice and black bean, which is prepared by filtration and concentration under reduced pressure, in a proportion of 0.1 to 30% by weight based on the total weight of the cosmetic / RTI > delete delete The cosmetic composition according to claim 1, wherein the cosmetic composition is for skin whitening. The cosmetic composition according to claim 1, wherein the cosmetic composition is for improving wrinkles. delete The cosmetic composition according to claim 1, wherein the cosmetic composition is for improving acne. The cosmetic composition according to claim 1, wherein the cosmetic composition is for improving atopy. A) mixing 1 to 30 parts by weight of dried and pulverized pomegranate, tomato, broccoli, fortified wormwood, turmeric, soybean, garlic, ginseng, black rice and black bean respectively;
B) adding a cellulase to the mixture together with 1 to 10 times the weight of the solvent and treating with the enzyme for 12 to 24 hours;
C) fermenting for 48 to 72 hours by adding yeast (saccharomyces cerevisiae); And
D) filtering and then concentrating under reduced pressure.