JP6007188B2 - Cosmetic composition comprising Honda walla extract, mill extract and wakame extract - Google Patents

Description

  The present invention relates to a cosmetic composition containing a gulfweed extract, a sea staghorn extract, and a brown seaweed extract as active ingredients. The present invention relates to a cosmetic composition containing one or more selected from the group consisting of an extract, a mill extract and a wakame extract, having an excellent antioxidant effect and improving skin elasticity and wrinkles (wrinkles).

  The skin is roughly divided into three layers, the epidermis, dermis, and subcutaneous adipose tissue in order from the outside, and functions to protect the human body from physical and chemical stimuli from the external environment. In particular, the skin regulates that about 65-70% of the water that the human body has is evaporated outside the body. Among them, the epidermis is divided into the stratum corneum, granule layer, spiny layer, and basal layer from the outside, and the stratum corneum of the epidermis contains about 10 to 20% water and exists in the outermost body of the human body and from the body. In addition, the excessive water penetration from the outside is blocked (J. Invest. Dermatol. 80 (Suppl.), 44-49.1983). The dermis is composed of a papillary layer and a reticular layer, and the papillary layer is composed of connective tissue, which is firm and elastic due to collagen fibers and partially elastic fibers (skin elasticity ( It plays an important role in wrinkles). Subcutaneous tissue is a layer composed of ligated tissue, sometimes also referred to as adipose tissue layer. The subcutaneous tissue is basically composed of loose connective tissue scattered in the subcutaneous fat layer, and such fat cells serve as a buffer for preserving and protecting heat and store heat.

  There are two main causes of skin aging: the first is the result of an aging phenomenon that is unavoidable due to the aging of endogenous aging, and the second is extrinsic aging, typically long-term sunlight. There are photoaging and active oxygen aging, which refers to skin damage as a result of exposure. The stratum corneum begins to overgrow while being damaged by the ultraviolet rays in sunlight, and as a result, photoaging occurs while the thickness of the stratum corneum increases. Moreover, the collagen-producing ability of the dermis layer decreases due to damage caused by ultraviolet rays, and as a result, the dermis layer gradually becomes thinner and thicker and deeper wrinkles than endogenous aging are generated. It is a well-known fact from numerous studies that skin aging is associated with reactive oxygen species (ROS). The active oxygen theory of aging was proposed by Harman in 1956, and the various active oxygens that are incidentally generated during normal metabolic processes have caused oxidative damage to biological components, and this damage has accumulated. It will lead to aging and death. Although the damage caused by active oxygen is dealt with by the defense ability of the living body, since the protection is not 100% complete, it will be affected by some of the active oxygen. This adverse effect occurs slowly and occasionally in years or throughout life, accumulating to reduce the function of skin cells and tissues, thus causing disease and aging. Research on cosmetics aimed at improving the reduction of skin elasticity and improving wrinkles is actively underway, while the desire for younger consumers of skin aging and the expectation for cosmetics are increasing.

  In general, cosmetics are intended to clean the body, to make yourself beautiful and attractive, and to protect the skin and hair from UV rays, dryness, etc., and prevent aging. In recent years, with the development of industrial society and the increase in social activities, the desire to actively express themselves has increased, while the desire to use cosmetics to maintain and maintain their own skin is greatly increased. doing. As a result, various forms and forms of foundations and color cosmetics have been researched and developed, among which skin elasticity improving and wrinkle improving cosmetics are recognized as the most important products in various categories.

  Therefore, a variety of cosmetic ingredients have been developed to improve skin wrinkles and skin elasticity, and natural plant-derived ingredients are attracting more attention than animal ingredients. . Seaweed extracts that contain a large amount of good nutrients while maintaining vitality in the underwater extreme environment are considered highly preferred by consumers who want a stronger effect. In fact, seaweed is an excellent source of vitamins and minerals for food, so if you apply it to your skin, it will be a good material that can immediately transmit its effects to your skin.

Therefore, as a result of repeated research on the skin efficacy of seaweed extract, the present inventor has found that Honda Walla extract, Mill extract and Wakame extract improve skin oxidative stress and have an effect of improving skin wrinkles. As a result, the present invention has been completed.
Accordingly, an object of the present invention is to provide a cosmetic composition having an excellent antioxidant effect, skin elasticity and wrinkle improving effect.

  In order to achieve the above object, the present invention provides a cosmetic composition for improving skin antioxidant, skin elasticity and wrinkles with an active ingredient containing a Honda Walla extract, a Mill extract and a Wakame extract.

  The cosmetic composition of the present invention contains a Honda walla extract, a mill extract and a wakame extract to enhance skin antioxidant power and is excellent in skin elasticity improving effect and wrinkle improving effect.

It is a graph which shows the damage protection effect of the stratum corneum formation cell by the ultraviolet-ray of the Honda walla extract of this invention, a mill extract, and a wakame extract.

The present invention contains at least one selected from the group consisting of Honda Walla Extract, Mill Extract and Wakame Extract as active ingredients.
Hereinafter, the present invention will be described in more detail.

  In the present invention, the honda walla (SARGASSUM FULLVELUM) used as an active ingredient is apparently divided into roots, stems, and leaves. The stem has a triangular prism shape or a triangular shape and can be grown. The leaves emerge from the stem toward the base and bend, forming a cocoon-like or elliptical shape and producing a midrib up to the center of the leaf. The upper leaves are needle-shaped and have serrated protrusions on the edges, and bubbles form from the stem. It is dark yellowish brown and can be seen on all coasts of Korea. The main ingredients are alginic acid, chlorophyll ac, β-carotene, fucoxanthin, laminarin, mannitol, and food when analyzing the raw of Honda walla The amount of fiber and fat is very low, but when processed in powder form, the amount of dietary fiber and fat is 30% or more. Honda Walla is a relatively high amount of dietary fiber rather than sugar. In Japan, many types of common honda are edible, used as raw materials for seaweed industry such as alginic acid, and used as fertilizer. Alginic acid helps digestive action of the gastrointestinal tract and helps bowel movement, and is said to be good for constipation and effective in preventing colorectal cancer. High mineral content, good for high blood pressure and osteoporosis, high iodine content, good for thyroid dysfunction.

  The mill (CODIUM FRAGILE) used as an active ingredient in the present invention has been recorded as having a smooth touch on the “Guan yama fish score”, a color of bluish black, a taste of light white and kimchi. You can see what kimchi flavoring material was used for. In Japan, it is called “Kaimatsu” in the sense of a pine that lives in the sea. The height is 10 to 30 cm, the thickness is 1.5 to 3 mm, and the lower side is thicker than the upper side. The branches are deer horns, break up and grow straight, both reach the same height and become fan-shaped. The surface is soft like a villus, and when viewed under a microscope, a structure like a colorless and transparent thread is irregularly drawn inside the branches. The younger ones have hair on the front, especially on the upper side, but then fall off, leaving a trace of the hair disappearing. The whole structure is a structure in which a large number of cell nuclei are contained in a single cell connected together without a membrane between cells. The sac containing the spore has a rod-like shape, is 5 to 7 times as long as its thickness, and has a sharp tip. It contains a lot of dietary fiber and contains a large amount of minerals such as calcium, iron, vitamin A, vitamin C, and niacin.

  Wakame (UNDARIA PINNATIFIDA) used in the present invention is a foliate plant with distinct roots, stems and leaves. It is distributed along the entire coast of Korea, but is not distributed in regions that are strongly affected by cold and warm currents. Inhabit the rocks near the low tide line, but the southern region tends to be deeper and the northern region shallower. It is mainly collected from winter to spring and has the best taste at this time. Breeds from spring to summer. It is the main food for abalone and turban shell and is mainly used for food only in places such as Korea, Japan and China. Rich in dietary fiber, potassium, calcium, iodine, etc., it has been used habitually for excellence in metabolism, after-partum cooking, prevention of constipation / obesity, and iron / calcium supplementation. There is a record that it has already been exported to China since the Goryeo period. “Korean rice cake” is recorded as “Wakame is widely preferred without precious rice. Its taste is salty and if you eat it for a long time, you can just eat it.” In the 12th year of Bunjong in the 11th year of Goryeo (1058 Seishi), he gave a 霍霍 field (a place to pick seaside seaweed), and in the reign of Tadanobu King in the 26th year of Goryeo (1301) Wakame was dedicated to the former Empress. “Tokaikan” says “Seafood is cold in nature and tastes salty and has no poison. The effect is to cure the lack of suffocation while fever is generated, and to make the urine go well.” There is a record to say. The dried product is protein 12.7%, carbohydrate 52%, fat 1.1%, calcium 1300mg%, vitamin A 140IU, vitamin B1 0.11mg%, vitamin B2 0.14mg%, nicotinic acid 10mg%, vitamin C Contains 15 mg%. Wakame is a strong alkaline food that has an excellent calcium content and is comparable to milk powder, and contains 100 mg% iodine. Wakame is a sticky substance containing alginic acid, and its root content is lower than leaves and stems. The main carotenoid pigments of wakame are fucoxanthine and violaxanthine, and there are also lutein and β-carotene.

  The Honda Walla extract, mill extract and wakame extract of the present invention can be produced by methods routine in the art. Specifically, for example, freeze-dried Honda Walla, mill, and seaweed can be obtained by distillation with steam, followed by extraction and purification using glycerin as a solvent.

  The cosmetic composition of the present invention contains Honda walla extract, mill extract and wakame extract in an amount of 0.001 to 30% by weight based on the total weight of the composition. This is because if the amount is less than 0.001% by weight, an obvious effect cannot be expected, and if it exceeds 30% by weight, an apparent increase in the effect due to an increase in the content does not appear.

  When the cosmetic composition of the present invention contains a Honda Walla extract, a Mill extract and a Wakame extract, the effects of skin antioxidant, wrinkle improvement and elasticity improvement are further increased, Honda Walla extract, Mill extract and When the mixture of three kinds of wakame extract is contained, the skin antioxidant, wrinkle improvement and elasticity improvement effects are most excellent due to the rising action between the active ingredients.

  The skin external preparation composition according to the present invention contains a cosmetically and dermatologically acceptable medium and / or base. This is all dosage forms suitable for topical application, for example solutions, gels, solids or dry kneaded products, emulsions, suspensions, microemulsions, microcapsules, microfruits obtained by dispersing the oil phase in the aqueous phase. It can be provided in the form of granulocytes or ionic (liposomes) and / or non-ionic vesicle dispersions, or in the form of creams, skins, lotions, powders, ointments, sprays or conceal sticks. These compositions can be prepared by conventional methods in the art. Further, the external preparation composition for skin according to the present invention can be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

  Skin external preparation composition according to the present invention comprises fatty substances, organic solvents, solubilizers, thickeners and gelling agents, softeners, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants. Water, ionic or non-ionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, It may contain adjuvants ordinarily used in the cosmetics or dermatological field such as lipid vesicles or any other ingredient normally used in cosmetics. These adjuvants are introduced in a content generally used in the cosmetics or dermatological fields.

  EXAMPLES Hereinafter, although an Example and a test example are given and this invention is demonstrated in more detail, this invention is not limited to these examples.

[Production Example 1] Manufacture of Honda Walla Extract 1 kg of freeze dried Honda Walla was distilled with steam for 12 hours. The distilled product was 70% glycerinized, and it was subjected to cold immersion at 4 ° C for 7 days, followed by purification to obtain.

[Production Example 2] Manufacture of mill extract 1 kg of freeze-dried mill was distilled with steam for 12 hours. The distilled product was 70% glycerinized, and it was subjected to 7-day chilling at 4 ° C, followed by purification to obtain.

[Production Example 3] Production of wakame extract 1 kg of freeze-dried wakame was distilled with steam for 12 hours, and the distilled product was 70% glycerinized, soaked at 4 ° C for 7 days, and purified to obtain.

[Production Example 4] Manufacture of a mixed extract of Honda Walla, mill and seaweed Lyophilized Honda Walla, Mill and Seaweed were mixed at a 1: 1: 1 weight ratio and distilled with steam for 12 hours. The distilled product was 70% glycerin. The product was cooled and soaked at 4 ° C. for 7 days, and purified to obtain.

[Production Example 5] Manufacture of mixed extract of Honda walla and mill Lyophilized Honda walla and mill were mixed at a 1: 1 weight ratio and distilled with steam for 12 hours, and the distilled product was 70% glycerinized for 7 days at 4 ° C. The product was refined and collected after being chilled in the water.

[Production Example 6] Manufacture of mixed extract of honda walla and seaweed Lyophilized honda walla and wakame were mixed at a 1: 1 weight ratio and distilled with steam for 12 hours. The distilled product was 70% glycerinized for 7 days at 4 ° C. The product was refined and collected after being chilled in the water.

[Production Example 7] Production of mill and wakame mixed extract Lyophilized mill and wakame were mixed at a 1: 1 weight ratio and distilled with steam for 12 hours, and the distilled product was 70% glycerinized for 7 days at 4 ° C. The product was refined and collected after being chilled in the water.

[Test Example 1] Protective effect of cell damage by ultraviolet rays 1 × 10 ⁴ keratinocytes separated from human epidermal tissue are placed in each well of a 96-well cell culture plate and allowed to adhere for 24 hours. It was. After 18 hours, the culture solution was removed, and 50 μL phosphate buffered saline (PBS) was added to each well. The keratinocytes were irradiated with ultraviolet rays 30 mJ / cm ² using an ultraviolet B (UV B) lamp (Model: F15T8, UV B 15W, Sankyo Dennki, Japan), and then PBS was reduced to each keratinocyte. 200 [mu] L of the culture solution (keratinocyte growth media, Clonetics, BioWhittacker, MD, USA) was added. Here, the Honda Walla extract, Mill extract, Wakame extract or mixed extract of the above Production Examples 1 to 7 were treated and cultured for 20 hours. After a certain period of time, an appropriate amount of the culture upper layer solution was taken, and the amount of lactate dehydrogenase (LDH), which is an indicator enzyme, was determined for 20 hours of cell damage. The amount of LDH in the culture upper layer solution was measured using Cytox 96 non-radioactive cytotoxity assay ^™ kit of Promega (Madison, WI, USA). The LDH secretion rate (%) can be obtained by fixing the vehicle as a control group at 100 and calculating the relative value of each group. The result is shown in FIG.

As can be seen in FIG. 1, as a result of comparing the group treated with the extract of Honda walla extract, mill extract and seaweed extract with the group treated with the mixed extract of Honda walla, mill and seaweed extract, When each wakame extract was treated, the increase in LDH due to human keratinocyte damage due to ultraviolet rays could be suppressed to some extent, but the group treated with two active ingredients more significantly reduced the increase in LDH. . In particular, treatment with a mixed extract of Honda Walla, Mill and Wakame reduced the increase in LDH to a similar degree to the control group (vehicle) that was not treated with UV light.
Therefore, the Honda Walla extract, mill extract and wakame extract of the present invention are excellent in the effect of protecting skin cells against external stimuli such as ultraviolet rays.

Reference Examples 1-3 and 5-7, Example 4, and Comparative Example 1]
The cream dosage forms of Reference Examples 1 to 3 and 5 to 7 , Example 4, and Comparative Example 1 were produced with the compositions shown in Table 1 below (unit:% by weight).

<Reference Examples 1-3 and 5-7, a manufacturing method of Example 4, and Comparative Example 1>
1) The above components (1) to (12) were uniformly mixed while heating to 70 ° C. to produce an aqueous phase part.
2) The above components (15) to (20) were uniformly mixed while heating to 70 ° C. to produce an oil phase part.
3) The oil phase part of 2) above was added to the water phase part of 1) above, and homomixed at 7,200 rpm for 6 minutes.
4) The mixture of 3) was cooled to room temperature.

[Test Example 2] Skin elasticity enhancement effect In order to test the elasticity enhancement effect on actual human skin, the following tests were conducted using Reference Examples 1 to 3 and 5 to 7 , Example 4 and Comparative Example 1. . Twenty women aged 25 to 35 years were applied to the face for 8 weeks twice a day in the morning and evening, and then the skin elasticity improving effect was measured using a skin elasticity measuring device (Cumeter SEM 575, C + K Electronic Co., Germany). The results are shown in Table 2 below. The result value means the skin elasticity of the skin elasticity meter.

As can be seen from the results in Table 2 above, the group treated with the extract of Honda Walla, Mill and Wakame alone increased skin elasticity. The skin elasticity enhancing effect of the group treated with these mixtures was further improved. In particular, the group treated with a mixture of Honda Walla, Mill and Wakame Extract had the most excellent skin elasticity enhancing effect.
Therefore, the Honda Walla extract, mill extract, and seaweed extract of the present invention were excellent in the effect of enhancing skin elasticity.

[Test Example 3] Collagen production promoting effect For the comparison of collagen production ability, no substance treatment was performed as a negative control group, and vitamin C was used as a positive control group. Using a fibroblast cell line hs68, the ability to produce type 1 procollagen and the ability to suppress collagenase synthesis were compared.

FALCON48-After fibroblasts were spread on 5 × 10 ⁴ / well on a well plate and attached, the extract of Honda walla extract, mill extract, wakame extract or a mixture thereof was added. Treated at 1 ppm concentration. After culturing for 24 hours, the amount of type 1 procollagen produced in the culture layer solution was determined using a type 1 pN collagen EIA kit (kit). After correcting the production amount of type 1 procollagen to the total protein amount, each control group was compared with the experimental group. The results are shown in Table 3 below.

As can be seen from the results in Table 3 above, the group treated with the extract of Honda Walla, Mill and Wakame alone promoted the production of collagen. The effect of promoting the production of collagen in the group treated with these mixtures was further increased. In particular, the group treated with a mixture of Honda walla, mill and wakame extract had the most excellent collagen production promoting effect.
Therefore, the Honda Walla extract, mill extract, and seaweed extract of the present invention promoted collagen production and were excellent in skin wrinkle improvement effect.

[Test Example 4] Collagenase synthesis inhibitory effect No substance was treated as a negative control group and retinoic acid was treated as a positive control group to measure collagenase synthesis inhibitory ability.
FALCON48-well (well) plate was attached with 5 × 10 ⁴ / well fibroblasts, irradiated with UVB 15 mJ / cm ² , Honda walla extract, mill extract, wakame Extracts or mixed extracts thereof were treated at a concentration of 1 ppm. After culturing for 24 hours, the amount of MMP-1 (matrix metalloproteinases-1), which is one of collagenases, was measured in the culture upper layer solution using an MMP-1 ELISA kit (kit). The amount of MMP-1 was corrected to the total protein mass. The results are summarized in Table 4 below.

As can be seen from the results in Table 4 above, the group treated with the extract of Honda, mill and wakame alone inhibited collagenase synthesis. The effect of inhibiting the synthesis of collagenase in the group treated with these mixtures was further improved. In particular, the group treated with a mixture of Honda walla, mill and wakame extract had the most excellent collagenase synthesis inhibitory effect.
Therefore, the Honda walla extract, mill extract, and seaweed extract of the present invention effectively suppressed collagenase synthesis and were excellent in skin wrinkle improvement effect.

Claims (2)

Sargassum, a mill and Waka extract skin elasticity for enhancing and wrinkle cosmetic composition containing as an active ingredient of a mixture of the eye,
A cosmetic composition for enhancing skin elasticity and improving skin wrinkles, wherein the mixture contains Honda Walla, Mill and Wakame in the same weight ratio .   The cosmetic composition for enhancing skin elasticity and improving skin wrinkles according to claim 1, wherein the extract is contained in an amount of 0.001 to 30% by weight based on the total weight of the composition.

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