CN108066210A - It is a kind of to utilize ground Folium Camelliae sinensis fermented product of edible mushroom and preparation method and application - Google Patents
It is a kind of to utilize ground Folium Camelliae sinensis fermented product of edible mushroom and preparation method and application Download PDFInfo
- Publication number
- CN108066210A CN108066210A CN201711413404.3A CN201711413404A CN108066210A CN 108066210 A CN108066210 A CN 108066210A CN 201711413404 A CN201711413404 A CN 201711413404A CN 108066210 A CN108066210 A CN 108066210A
- Authority
- CN
- China
- Prior art keywords
- ground
- edible mushroom
- folium camelliae
- camelliae sinensis
- fermented product
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
- Mycology (AREA)
- Botany (AREA)
- Public Health (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Animal Behavior & Ethology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Dermatology (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
- Cosmetics (AREA)
Abstract
The present invention provides a kind of using ground Folium Camelliae sinensis fermented product of edible mushroom and preparation method and application, which has good moistening effect, to skin without any stimulation, can be used as skin care item moisturizer；Its technical solution is：Make ground tea leaf fermentation using edible mushroom and obtain.
Description
Technical field
The present invention relates to a kind of ground Folium Camelliae sinensis fermented products, are to be related to a kind of ground tea leaf fermentation using edible mushroom specifically
Object, preparation method and application the invention further relates to the ground Folium Camelliae sinensis fermented product, belongs to cosmetic technical field.
Background technology
Fermentation refers to the process of that microorganism utilizes the enzyme decomposition itself carried or synthesis of organic substance, representative fermentation
Microorganism includes withered grass bacterium, lactic acid bacteria, Aspergillus, saccharomycete, mould, acetic acid bacteria, mushroom mycelium etc..Recently, in cosmetics
Just carry out whitening extensively for increasingly being paid close attention to using natural material making fermented food and cosmetics, improve skin wrinkle in field
The research of line, anti-oxidant, moisturizing, enhancing elasticity etc..
The extract of fermentate or fermentate makes natural material without using other any solvents by fermentation and maturation process
In the sugar transition that contains into alcohol, flavonoids in natural material, vitamin C, citrin, amino acid, catechin can be improved
Etc. effective ingredients extraction efficiency.In addition, sometimes for the natural material with irritation or toxicity, by fermenting, extraction method makes
Natural material is stable or reduces toxicity or converts it into stable derivatives, can greatly improve beauty functions.In addition,
The lower-molecular substance easily absorbed is converted into, from unstable or inertia Forms Transformation by the zymotechnique of lactic acid bacteria and yeast etc.
During for activity morphology, the effect of can greatling improve natural material, strengthens moisturizing, enhancing elasticity, whitening, improvement skin
The effect of skin wrinkle.
Edible mushroom contains abundant protein and amino acid, and content is several times to tens times of general vegetables and fruit.
8 kinds of trace elements necessary to 18 kinds of amino acid and human body containing constitutive protein matter in edible mushroom.Content is few in cereal foods
Lysine, content is also quite abundant in edible mushroom.Edible mushroom fat content is very low, accounts for the 0.2%-3.6% of dry product weight,
And wherein 74-83% is the beneficial unrighted acid of health.Edible mushroom also contains vitamin, VB1, the V12 being rich in,
All it is higher than meat；Mushroom Vd original contents are up to 128 international units, are 8 times of seaweed.Edible mushroom is also rich in a variety of mineral elements:
Phosphorus, potassium, sodium, calcium, iron, zinc, magnesium, manganese etc. and some other trace element.
In edible mushroom containing bioactive substance such as macromolecule polysaccharide, β-glucose and RNA complexs, natural organic germanium,
Nucleic acid degradation product, cAMP and triterpene compound etc., to safeguarding that health has important utility value.The medicinal guarantor of edible mushroom
Strong value has:1. antitumaous effect, the polysaccharide body of edible mushroom can stimulate the formation of antibody, improve and adjust the defence of internal body
Ability can reduce the incidence of Cucumber induced tumor, and have synergistic effect to Treated with Chemotherapeutic Drugs object.In addition it is rich in chestnut mushroom
Organic Selenium, selenium-supply food can be made, if long-term consumption, can almost prevent all cancerations.2. antibacterial, antivirus action.3. it drops
Blood pressure, reducing blood lipid, antithrombotic, anti-arrhythmia, cardiac stimulant etc..4. stomach invigorating, digestion-aid effect.5. relieving cough and asthma, phlegm-dispelling functions.⑥
Cholagogic, liver protection, removing toxic substances.It is 7. hypoglycemic.8. defaecation diuresis.9. immunological regulation.It is eaten by the health care that raw material produces and processes of edible mushroom
Product, health drink, wine and drug are largely used to medicinal and input health-product market.
Thamnolia vermicularia also known as Bai Xuecha, too white tea are similar to feverfew petal, and as white as snow, therefore named Lijing avenges tea, are grown on Lijing jade
The snowy region high mountain bryophyte band of imperial 4000 meters of snow mountain height above sea level or more.The chemical composition of Bai Xuecha has unusual health-care effect.
Its chemical composition is made of the inorganic matter of 3.5-7.0% and 93~96.5% organic matter.Inorganic mineral element in tealeaves
It is a variety of including phosphorus, potassium, sulphur, magnesium, manganese, fluorine, aluminium, calcium, sodium, iron, copper, zinc, selenium etc. there are about 27 kinds.Organic compound in tealeaves
Mainly there are protein, lipid, carbohydrate, amino acid, alkaloid, tea polyphenols, organic acid, pigment, fragrance component, dimension life
Element, saponin(e, sterol etc..Thamnolia vermicularia energy lowering blood pressure, weight-reducing, clearing away summerheat, cough-relieving of promoting the production of body fluid, clear liver and improve vision, exclude at restoring consciouness moistening lung
Toxin, soothing the liver fall fire, nourishing Yin and moistening lung, suitable for pneumonia cough, hypertension, neurasthenia, sphagitis, demented agitation etc..But
Application study in relation to thamnolia vermicularia in terms of cosmetics is also relatively fewer.
The content of the invention
For this purpose, present invention offer is a kind of using edible fungus fermented ground Folium Camelliae sinensis fermented product, this utilizes edible fungus fermented ground
Folium Camelliae sinensis fermented product has good moistening effect, to skin without any stimulation, can be used as skin care item moisturizer, can effectively improve
Scaly dry skin, tight and microgroove problem.
In order to achieve the above object, first technical solution provided by the invention is such：
A kind of ground Folium Camelliae sinensis fermented product using edible mushroom makes ground tea leaf fermentation using edible mushroom and obtains.
Further, above-mentioned a kind of ground Folium Camelliae sinensis fermented product using edible mushroom, the edible mushroom include Sparassis crispa, ash
Tree flower and Trichotoma matsutake.
Further, above-mentioned a kind of ground Folium Camelliae sinensis fermented product using edible mushroom, the edible mushroom further include kahikatea dew
Bacterium.
Further, a kind of above-mentioned ground Folium Camelliae sinensis fermented product using edible mushroom, the edible mushroom further include summer truffle.
Second technical solution provided by the invention is such：
Further, the preparation method of the above-mentioned ground Folium Camelliae sinensis fermented product using edible mushroom, comprises the following steps successively：
(1) seed liquor is prepared
Respectively by each fruit body of edible fungi aseptically clip tissue fragment, and be inoculated in inclined-plane culture respectively
Base, quiescent culture 8-10 days at 25-28 DEG C of temperature；Again aseptically from slant medium picking carry mycelia fine jade
Fat 10-20 blocks, are accessed again in sterile slant medium, and at 25~28 DEG C, light culture is after 12~15 days, illumination 3~4 days,
The strain that annesl is very fast and mycelial growth is neat is chosen, as parent species, is then seeded in seed culture medium, in temperature 26-28
DEG C, it under the conditions of shaking speed is 150-200r/min, cultivates 5-7 days, respectively obtains the seed liquor of each edible mushroom；
(2) by ground tealeaves Ultramicro-powder essence to 80-100 μm, the water of 2-4 times of quality is added in, is impregnated 1-2 days at 30-40 DEG C,
Centrifugation obtains ground tealeaves soaked extracting solution；
(3) each edible mushroom seed liquor that step (1) is prepared to gained mixes in advance according to isometric ratio, with connecing for 5-10%
In kind amount access high density fermentation culture medium 5L fermentation tanks, and the ground tealeaves soaked extracting solution for adding in step (2) starts fermentation training
It supports, the mass ratio of ground tealeaves soaked extracting solution and edible mushroom seed liquor is 3-6：1, it is 28-30 DEG C in temperature, rotating speed 100-
250r/min, throughput 0.5-1.5dm3Fermented and cultured 5-10 days under the conditions of/min carries out submerged fermentation and obtains edible mushroom hair
Ferment tealeaves mixture, centrifugation, take supernatant liquid filtering.
(4) carry out being concentrated under reduced pressure into moisture content under conditions of the filtrate that step (3) is obtained by filtration is below 60 DEG C and be less than
8%, it is then freeze-dried, obtained powder as Folium Camelliae sinensis fermented product.
Further, the preparation method of above-mentioned a kind of ground Folium Camelliae sinensis fermented product using edible mushroom, inclined-plane described in step (1)
Culture medium is PDA culture medium or PDPA synthetic mediums.
Further, the preparation method of above-mentioned a kind of ground Folium Camelliae sinensis fermented product using edible mushroom, seed described in step (1)
Culture medium is：PH6.0-6.5, based on its total mass fraction 100%, by glucose 1.5-2.5%, peptone 0.1-0.2%, ox
Meat extract 0.1-0.2%, KH2PO40.1-0.15%, MgSO40.1-0.15%, vitaminB10 .01-0.02%, surplus are water.
Further, a kind of preparation method of above-mentioned ground Folium Camelliae sinensis fermented product using edible mushroom, step are fermented described in (3)
Medium pH 5.5-6.0, based on its total mass fraction 100%, by glucose -3%, peptone 0.4-0.6%, yeast extract
0.2-0.4%, KH2PO40.15-0.3%, MgSO40.1-0.15%, vitaminB10 .01-0.02%, surplus are water.
The last one technical solution of the present invention is to provide the above-mentioned ground Folium Camelliae sinensis fermented product by the use of edible mushroom as skin care item
The application of moisturizer, mass percent of the moisture-keeping composition in skin care item are 0.1-20%.
Compared with prior art, technical solution provided by the invention has following technological merit：
1st, the ground Folium Camelliae sinensis fermented product that the application provides is to be obtained using the mycelium of edible mushroom by zymotechnique, production
Organic solvent is eliminated in the process, is avoided the negative effect that organic solvent generates human body, and using cold fermentation, is retained
To thermo-responsive ingredient；Its high-molecular compound is converted into degraded by the enzyme effect that ground tealeaves generates in by fermentation process
Object is closed, obtained nutritional ingredient is easier skin permeation, and combines the active ingredient of edible mushroom itself, and adding has skin
The substance of benefit, has effects that good moisturizing moisturizing.This tunning can be applied to eye cream, face cream, facial mask, lotion, gel,
In the skin protection cosmetics such as elite, good replenishing water and preserving moisture effect can be brought to these products, is effectively improved scaly dry skin, tight
With microgroove problem.
2nd, using multiple eating bacterium mixed fermentation tealeaves, multiple eating bacterium can generate the technical solution that the application provides
The enzyme system of different performance, several edible mushroom mutualisms, makes enzyme system eurythmy, and fermentation substrate is made to decompose more thorough, generates
A variety of small molecule active products, these activated products are synergistic, reach better moisturizing moisturizing effect.
Specific embodiment
With reference to embodiment, the invention will be further described, but does not form any limitation of the invention, any
In the modification for the limited number of time that scope of the invention as claimed is made, still in scope of the presently claimed invention.
Embodiment 1
A kind of ground Folium Camelliae sinensis fermented product using edible mushroom provided by the invention, using including Sparassis crispa, grifola frondosus and becoming less severe
Mushroom makes ground tea leaf fermentation and obtains.
Above-mentioned ground Folium Camelliae sinensis fermented product is passed sequentially through made from following step：
(1) edible mushroom seed liquor is prepared；
By Sparassis crispa fructification aseptically clip tissue fragment, PDA culture medium is inoculated in, it is quiet at 28 DEG C of temperature
Put culture 8 days；Aseptically agar 20 piece of the picking with mycelia from PDA culture medium accesses sterile PDA trainings again
It supports in base, at 25 DEG C, light culture is after 15 days, illumination 3 days, chooses annesl is very fast and mycelial growth is neat strain, as parent species,
It is then seeded in seed culture medium, in 28 DEG C of temperature, under the conditions of shaking speed is 150r/min, cultivates 7 days, obtain the embroidery
Coccus seed liquor；
By Grifolas frondosa germ fructification aseptically clip tissue fragment, PDA culture medium is inoculated in, at 28 DEG C of temperature
Quiescent culture 8 days；Aseptically agar 20 piece of the picking with mycelia from PDA culture medium, accesses sterile PDA again
In culture medium, at 25 DEG C, light culture is after 15 days, illumination 3 days, the strain that annesl is very fast and mycelial growth is neat is chosen, as mother
Kind, it is then seeded in seed culture medium, in 28 DEG C of temperature, under the conditions of shaking speed is 150r/min, cultivates 7 days, obtain institute
State grifola frondosus seed liquor；
By Trichotoma matsutake fructification aseptically clip tissue fragment, PDA culture medium is inoculated in, it is quiet at 28 DEG C of temperature
Put culture 8 days；Aseptically agar 20 piece of the picking with mycelia from PDA culture medium accesses sterile PDA trainings again
It supports in base, at 25 DEG C, light culture is after 15 days, illumination 3 days, chooses annesl is very fast and mycelial growth is neat strain, as parent species,
It is then seeded in seed culture medium, in 28 DEG C of temperature, under the conditions of shaking speed is 150r/min, cultivates 7 days, obtain the pine
Dried mushroom seed liquor；
Seed culture medium is described in step (1)：PH6.0, based on its total mass fraction 100%, by glucose 2.5%,
Peptone 0.1%, beef extract 0.2%, KH2PO40.1%th, MgSO40.15%, vitaminB10 .01%, surplus is water.
(2) by ground tealeaves Ultramicro-powder essence to 100 μm, the water of 2 times of quality is added in, is impregnated 1 day at 40 DEG C, centrifuged, obtain
Ground tealeaves soaked extracting solution；
(3) step (1) is prepared into each seed liquor of gained in advance according to volume ratio 1：1：1 mixing, with 10% inoculum concentration
It accesses in high density fermentation culture medium 5L fermentation tanks, and the ground tealeaves soaked extracting solution for adding in step (2) starts fermented and cultured, ground
Infusion of tea extracting solution and the mass ratio of edible mushroom seed liquor are 3：1, it is 30 DEG C, rotating speed 100r/min in temperature, throughput
For 1.5dm3Fermented and cultured 5 days under the conditions of/min carry out the mixture that submerged fermentation obtains edible fungus fermented ground tealeaves, centrifugation,
Take supernatant liquid filtering.
Fermentation medium pH 5.5 described in step (3), based on its total mass fraction 100%, by glucose 3%, albumen
Peptone 0.4%, yeast extract 0.4%, KH2PO40.15%th, MgSO40.15%, vitaminB10 .01%, surplus is water.
(4) it is 8% that the filtrate that step (3) is obtained by filtration, which carries out being concentrated under reduced pressure into moisture content under conditions of 60 DEG C, so
After be freeze-dried, obtained powder as Folium Camelliae sinensis fermented product.
Embodiment 2
A kind of ground Folium Camelliae sinensis fermented product using edible mushroom provided by the invention, using including summer truffle, Sparassis crispa, grifola frondosus
Make ground tea leaf fermentation with Trichotoma matsutake and obtain.
Above-mentioned ground Folium Camelliae sinensis fermented product is passed sequentially through made from following step：
(1) edible mushroom seed liquor is prepared
By Sparassis crispa fructification aseptically clip tissue fragment, PDPA synthetic mediums are inoculated in, in temperature 25
Quiescent culture 10 days at DEG C；10 pieces of the agar of mycelia is aseptically carried from picking on PDPA synthetic mediums, is connect again
Enter in sterile PDPA synthetic mediums, at 28 DEG C, light culture is after 12 days, illumination 4 days, and selection annesl is very fast and mycelial growth is whole
Neat strain as parent species, is then seeded in seed culture medium, in 26 DEG C of temperature, under the conditions of shaking speed is 200r/min,
Culture 5 days, obtains Sparassis crispa seed liquor；
By Grifolas frondosa germ fructification aseptically clip tissue fragment, PDPA synthetic mediums are inoculated in, in temperature
Quiescent culture 10 days at 25 DEG C；10 pieces of the agar of mycelia is aseptically carried from picking on PDPA synthetic mediums, again
It accesses in sterile PDPA synthetic mediums, at 28 DEG C, light culture is after 12 days, illumination 4 days, chooses that annesl is very fast and mycelial growth
Neat strain as parent species, is then seeded in seed culture medium, and in 26 DEG C of temperature, shaking speed is 200r/min conditions
Under, it cultivates 5 days, obtains grifola frondosus seed liquor；
By Trichotoma matsutake mushroom entity aseptically clip tissue fragment, PDPA synthetic mediums are inoculated in, in temperature
Quiescent culture 10 days at 25 DEG C；10 pieces of the agar of mycelia is aseptically carried from picking on PDPA synthetic mediums, again
It accesses in sterile PDPA synthetic mediums, at 28 DEG C, light culture is after 12 days, illumination 4 days, chooses that annesl is very fast and mycelial growth
Neat strain as parent species, is then seeded in seed culture medium, and in 26 DEG C of temperature, shaking speed is 200r/min conditions
Under, it cultivates 5 days, obtains Trichotoma matsutake seed liquor；
By summer truffle fructification aseptically clip tissue fragment, PDPA synthetic mediums are inoculated in, in temperature 25
Quiescent culture 10 days at DEG C；10 pieces of the agar of mycelia is aseptically carried from picking on PDPA synthetic mediums, is connect again
Enter in sterile PDPA synthetic mediums, at 28 DEG C, light culture is after 12 days, illumination 4 days, and selection annesl is very fast and mycelial growth is whole
Neat strain as parent species, is then seeded in seed culture medium, in 26 DEG C of temperature, under the conditions of shaking speed is 200r/min,
Culture 5 days, obtains summer truffle seed liquor；
Seed culture medium is described in step (1)：PH6.0, based on its total mass fraction 100%, by glucose 1.5%,
Peptone 0.2%, beef extract 0.1%, KH2PO40.15%th, MgSO40.1%, vitamin B1 0.02%, surplus is water.
(2) by ground tealeaves Ultramicro-powder essence to 80 μm, the water of 4 times of quality is added in, is impregnated 2 days at 30 DEG C, centrifuged, obtain ground
Infusion of tea extracting solution；
(3) step (1) is prepared into each seed liquor of gained in advance according to volume ratio 1：1：1：1 mixing, with 5% inoculum concentration
It accesses in high density fermentation culture medium 5L fermentation tanks, and the ground tealeaves soaked extracting solution for adding in step (2) starts fermented and cultured, ground
Infusion of tea extracting solution and the mass ratio of edible mushroom seed liquor are 6：1, it is 28 DEG C, rotating speed 250r/min in temperature, throughput
For 0.5dm3Fermented and cultured 10 days under the conditions of/min carry out the mixture that submerged fermentation obtains edible fungus fermented ground tealeaves, centrifugation,
Take supernatant liquid filtering.
Fermentation medium pH 5.5~6.0 described in step (3), based on its total mass fraction 100%, by glucose 2%,
Peptone 0.6%, yeast extract 0.2%, KH2PO40.3%th, MgSO40.1%, vitaminB10 .02%, surplus is water.
(4) it is 5% that the filtrate that step (3) is obtained by filtration, which carries out being concentrated under reduced pressure into moisture content under conditions of 50 DEG C, so
After be freeze-dried, obtained powder as Folium Camelliae sinensis fermented product.
Embodiment 3
A kind of ground Folium Camelliae sinensis fermented product using edible mushroom provided by the invention, using including kahikatea dew bacterium, Sparassis crispa, ash tree
Flower and Trichotoma matsutake make ground tea leaf fermentation and obtain.
Above-mentioned ground Folium Camelliae sinensis fermented product is passed sequentially through made from following step：
(1) edible mushroom seed liquor is prepared
By Sparassis crispa fructification aseptically clip tissue fragment, PDPA synthetic mediums are inoculated in, in temperature 26
Quiescent culture 9 days at DEG C；15 pieces of the agar of mycelia is aseptically carried from picking on PDPA synthetic mediums, is accessed again
In sterile PDA culture medium or PDPA synthetic mediums, at 26 DEG C, light culture is after 14 days, illumination 4 days, and it is very fast to choose annesl
And the strain that mycelial growth is neat, it as parent species, is then seeded in seed culture medium, in 27 DEG C of temperature, shaking speed is
Under the conditions of 180r/min, cultivate 6 days, obtain the Sparassis crispa seed liquor；
By Grifolas frondosa germ fructification aseptically clip tissue fragment, PDPA synthetic mediums are inoculated in, in temperature
Quiescent culture 9 days at 26 DEG C aseptically carry 15 pieces of the agar of mycelia from picking on PDPA synthetic mediums, connect again
Enter in sterile PDA culture medium or PDPA synthetic mediums, at 26 DEG C, light culture is after 14 days, illumination 4 days, choose annesl compared with
Fast and neat mycelial growth strain, as parent species, is then seeded in seed culture medium, in 27 DEG C of temperature, shaking speed is
Under the conditions of 180r/min, cultivate 6 days, obtain the edible mushroom seed liquor；
By Trichotoma matsutake mushroom entity aseptically clip tissue fragment, PDPA synthetic mediums are inoculated in, in temperature
Quiescent culture 9 days at 26 DEG C；15 pieces of the agar of mycelia is aseptically carried from picking on PDPA synthetic mediums, is connect again
Enter in sterile PDA culture medium or PDPA synthetic mediums, at 26 DEG C, light culture is after 14 days, illumination 4 days, choose annesl compared with
Fast and neat mycelial growth strain, as parent species, is then seeded in seed culture medium, in 27 DEG C of temperature, shaking speed is
Under the conditions of 180r/min, cultivate 6 days, obtain the Trichotoma matsutake bacterium seed liquor；
Kahikatea is revealed into mushroom entity aseptically clip tissue fragment, PDPA synthetic mediums are inoculated in, in temperature
Quiescent culture 9 days at 26 DEG C；15 pieces of the agar of mycelia is aseptically carried from picking on PDPA synthetic mediums, is connect again
Enter in sterile PDA culture medium or PDPA synthetic mediums, at 26 DEG C, light culture is after 14 days, illumination 4 days, choose annesl compared with
Fast and neat mycelial growth strain, as parent species, is then seeded in seed culture medium, in 27 DEG C of temperature, shaking speed is
It under the conditions of 180r/min, cultivates 6 days, obtains the kahikatea dew bacterium seed liquor；
Seed culture medium is described in step (1)：PH6.3, based on its total mass fraction 100%, by glucose 2%, egg
White peptone 0.15%, beef extract 0.15%, KH2PO40.12%th, MgSO40.12%, vitaminB10 .015%, surplus is water.
(2) by ground tealeaves Ultramicro-powder essence to 90 μm, the water of 3 times of quality is added in, is impregnated 1.5 days at 35 DEG C, centrifuged, obtain
Ground tealeaves soaked extracting solution；
(3) step (1) is prepared into each seed liquor of gained in advance according to volume ratio 1：1：1：1 mixing, with 7% inoculum concentration
It accesses in high density fermentation culture medium 5L fermentation tanks, and the ground tealeaves soaked extracting solution for adding in step (2) starts fermented and cultured, ground
Infusion of tea extracting solution and the mass ratio of edible mushroom seed liquor are 5：1, it is 28 DEG C, rotating speed 200r/min in temperature, throughput
For 1.0dm3Fermented and cultured 8 days under the conditions of/min carry out the mixture that submerged fermentation obtains edible fungus fermented ground tealeaves, centrifugation,
Take supernatant liquid filtering.
Fermentation medium pH 5.8 described in step (3), based on its total mass fraction 100%, by glucose 2.5%, egg
White peptone 0.5%, yeast extract 0.25%, KH2PO40.2%th, MgSO40.12%, vitaminB10 .015%, surplus is water.
(4) it is 6% that the filtrate that step (3) is obtained by filtration, which carries out being concentrated under reduced pressure into moisture content under conditions of 55 DEG C, so
After be freeze-dried, obtained powder as Folium Camelliae sinensis fermented product.
Embodiment 4
A kind of ground Folium Camelliae sinensis fermented product using edible mushroom provided by the invention, using including summer truffle, kahikatea dew bacterium, silk ball
Bacterium, grifola frondosus and Trichotoma matsutake make ground tea leaf fermentation and obtain.
Above-mentioned ground Folium Camelliae sinensis fermented product is passed sequentially through made from following step：
(1) edible mushroom seed liquor is prepared
By Sparassis crispa fructification aseptically clip tissue fragment, PDA culture medium is inoculated in, it is quiet at 27 DEG C of temperature
Put culture 9 days；18 pieces of the agar of mycelia is aseptically carried from picking in PDA culture medium or PDPA synthetic mediums,
It accesses again in sterile PDA culture medium, at 27 DEG C, light culture is after 14 days, illumination 4 days, chooses that annesl is very fast and mycelial growth
Neat strain as parent species, is then seeded in seed culture medium, and in 28 DEG C of temperature, shaking speed is 170r/min conditions
Under, it cultivates 7 days, obtains the edible mushroom seed liquor；
Kahikatea is revealed into mushroom entity aseptically clip tissue fragment, PDA culture medium is inoculated in, at 27 DEG C of temperature
Quiescent culture 9 days；The agar 18 of mycelia is aseptically carried from picking in PDA culture medium or PDPA synthetic mediums
Block is accessed again in sterile PDA culture medium, and at 27 DEG C, light culture is after 14 days, illumination 4 days, and selection annesl is very fast and mycelia gives birth to
Long neat strain, as parent species, is then seeded in seed culture medium, and in 28 DEG C of temperature, shaking speed is 170r/min items
It under part, cultivates 7 days, obtains the kahikatea dew seed liquor；
By Grifolas frondosa germ fructification aseptically clip tissue fragment, PDA culture medium is inoculated in, at 27 DEG C of temperature
Quiescent culture 9 days；The agar 18 of mycelia is aseptically carried from picking in PDA culture medium or PDPA synthetic mediums
Block is accessed again in sterile PDA culture medium, and at 27 DEG C, light culture is after 14 days, illumination 4 days, and selection annesl is very fast and mycelia gives birth to
Long neat strain, as parent species, is then seeded in seed culture medium, and in 28 DEG C of temperature, shaking speed is 170r/min items
Under part, cultivate 7 days, obtain the grifola frondosus seed liquor；
By Trichotoma matsutake mushroom entity aseptically clip tissue fragment, PDA culture medium is inoculated in, at 27 DEG C of temperature
Quiescent culture 9 days；The agar 18 of mycelia is aseptically carried from picking in PDA culture medium or PDPA synthetic mediums
Block is accessed again in sterile PDA culture medium, and at 27 DEG C, light culture is after 14 days, illumination 4 days, and selection annesl is very fast and mycelia gives birth to
Long neat strain, as parent species, is then seeded in seed culture medium, and in 28 DEG C of temperature, shaking speed is 170r/min items
Under part, cultivate 7 days, obtain Trichotoma matsutake seed liquor；
By summer truffle fructification aseptically clip tissue fragment, PDA culture medium is inoculated in, it is quiet at 27 DEG C of temperature
Put culture 9 days；18 pieces of the agar of mycelia is aseptically carried from picking in PDA culture medium or PDPA synthetic mediums,
It accesses again in sterile PDA culture medium, at 27 DEG C, light culture is after 14 days, illumination 4 days, chooses that annesl is very fast and mycelial growth
Neat strain as parent species, is then seeded in seed culture medium, and in 28 DEG C of temperature, shaking speed is 170r/min conditions
Under, it cultivates 7 days, obtains the summer truffle seed liquor；
Seed culture medium is described in step (1)：PH6.2, based on its total mass fraction 100%, by glucose 2%, egg
White peptone 0.16%, beef extract 0.16%, KH2PO40.13%th, MgSO40.13%, vitaminB10 .016%, surplus is water.
(2) by ground tealeaves Ultramicro-powder essence to 90 μm, the water of 3 times of quality is added in, is impregnated 1.5 days at 36 DEG C, centrifuged, obtain
Ground tealeaves soaked extracting solution；
(3) step (1) is prepared into each seed liquor of gained in advance according to volume ratio 1：1：1：1：1 mixing, with 7% inoculation
In amount access high density fermentation culture medium 5L fermentation tanks, and the ground tealeaves soaked extracting solution for adding in step (2) starts fermented and cultured,
The mass ratio of ground tealeaves soaked extracting solution and edible mushroom seed liquor is 4：1, it is 30 DEG C, rotating speed 150r/min in temperature, ventilation
It measures as 0.8dm3Fermented and cultured 7 days under the conditions of/min carry out the mixture that submerged fermentation obtains edible fungus fermented ground tealeaves, from
The heart takes supernatant liquid filtering.
Fermentation medium pH 5.9 described in step (3), based on its total mass fraction 100%, by glucose 2.4%, egg
White peptone 0.6%, yeast extract 0.3%, KH2PO40.18%th, MgSO40.13%, vitaminB10 .016%, surplus is water.
(4) it is 8% that the filtrate that step (3) is obtained by filtration, which carries out being concentrated under reduced pressure into moisture content under conditions of 60 DEG C, so
After be freeze-dried, obtained powder as Folium Camelliae sinensis fermented product.
Ground provided by the invention Folium Camelliae sinensis fermented product is more using Sparassis crispa, grifola frondosus, Trichotoma matsutake, kahikatea dew bacterium and summer truffle
Tealeaves, multiple eating bacterium can generate the enzyme system of different performance with planting edible mushroom mixed fermentation, and several edible mushroom mutualisms make enzyme
It is eurythmy, fermentation substrate is made to decompose more thorough, generate a variety of small molecule active products, the collaboration of these activated products increases
Effect, reaches better moisturizing moisturizing effect.
It is provided by the invention to be added to by the use of edible fungus fermented ground Folium Camelliae sinensis fermented product as skin-care product additive such as eye
In the skin care item of the various dosage forms such as frost, face cream, facial mask, lotion, gel, elite, the better moisturizing moisturizing effect of product is assigned, is had
Effect improves scaly dry skin, tight and microgroove problem；The mass percent of said composition is 0.1~20% in the skin care item.
Comparative example 1:Prepare common ground tea extract
Ground tea extract is prepared using conventional circumfluence distillation technique, method is as follows：By ground tealeaves Ultramicro-powder essence extremely
90 μm, the water of 10 times of quality is added in, 5h is heated at 70 DEG C and carries out circumfluence distillation, supernatant is separated with sediment, repeatedly
Extraction 3 times, is concentrated under reduced pressure under conditions of 55 DEG C after extract is filtered, is then freeze-dried, obtained powder is thamnolia vermicularia
The common extract of leaf.
Comparative example 2:Ground Folium Camelliae sinensis fermented product is prepared using Sparassis crispa
The preparation method of Sparassis crispa seed liquor and the method for fermented and cultured are the same as embodiment 4.
Comparative example 3:Ground Folium Camelliae sinensis fermented product is prepared using grifola frondosus
The preparation method of grifola frondosus seed liquor and the method for fermented and cultured are the same as embodiment 4.
Comparative example 4:Ground Folium Camelliae sinensis fermented product is prepared using Trichotoma matsutake
The preparation method of Trichotoma matsutake seed liquor and the method for fermented and cultured are the same as embodiment 4.
Comparative example 5:Ground Folium Camelliae sinensis fermented product is prepared using kahikatea dew bacterium
The preparation method of kahikatea dew bacterium seed liquor and the method for fermented and cultured are the same as embodiment 4.
Comparative example 6:Ground Folium Camelliae sinensis fermented product is prepared using summer truffle
The preparation method of summer truffle seed liquor and the method for fermented and cultured are the same as embodiment 4.
1 performance of keeping humidity of experimental example is tested
1st, test population：The subject of 60 dry skins, age 20-60 Sui, gender is random, is divided into 12 groups, every group 5
People.
2nd, the preparation of given the test agent：Ground tealeaves prepared by the ground Folium Camelliae sinensis fermented product of the preparation of embodiment 1-4, comparative example 1
Ground Folium Camelliae sinensis fermented product and Sodium Hyaluronate prepared by extract, comparative example 2-6 are configured to 1% aqueous solution with distilled water.
2nd, test method：
1) technical staff is taken using the experiment position of moisture content of skin test Corneometer CM825 measurement subjects
Average value is denoted as blank value.
2) subject uses moisture content of skin after given the test agent 0.5h, 1h, 2h, 4h, 6h is used continuously by technical staff
It tests Corneometer CM825 to measure 5 times, be averaged, record numerical value, the results are shown in Table 1；
1 moisture test result of table
| Test sample | 0h | 0.5h | 1h | 2h | 4h | 6h |
| 1 ground Folium Camelliae sinensis fermented product of embodiment | 35.22 | 64.38 | 56.04 | 51.79 | 49.71 | 49.31 |
| 2 ground Folium Camelliae sinensis fermented product of embodiment | 36.81 | 69.53 | 60.98 | 56.68 | 54.85 | 54.32 |
| 3 ground Folium Camelliae sinensis fermented product of embodiment | 35.65 | 67.88 | 59.21 | 55.67 | 53.45 | 52.71 |
| 4 ground Folium Camelliae sinensis fermented product of embodiment | 34.15 | 68.74 | 61.48 | 56.35 | 54.38 | 53.86 |
| 1 ground tea extract of comparative example | 34.43 | 51.23 | 44.52 | 41.01 | 39.60 | 38.92 |
| 2 ground Folium Camelliae sinensis fermented product of comparative example | 36.82 | 61.68 | 52.79 | 49.92 | 47.08 | 46.10 |
| 3 ground Folium Camelliae sinensis fermented product of comparative example | 37.90 | 61.45 | 53.54 | 50.87 | 48.03 | 47.22 |
| 4 ground Folium Camelliae sinensis fermented product of comparative example | 37.08 | 61.63 | 50.98 | 48.53 | 46.75 | 45.76 |
| 5 ground Folium Camelliae sinensis fermented product of comparative example | 36.72 | 61.21 | 50.68 | 48.19 | 46.67 | 45.82 |
| 6 ground Folium Camelliae sinensis fermented product of comparative example | 37.90 | 62.39 | 52.72 | 48.97 | 47.43 | 46.42 |
| Sodium Hyaluronate | 37.08 | 58.43 | 50.38 | 47.23 | 45.85 | 44.78 |
| Distilled water | 36.72 | 37.11 | 36.68 | 36.59 | 36.67 | 36.42 |
In addition, the applicant calculates the skin hydration rate after using given the test agent, calculation formula is hydration rate
={ (test value-blank value)/blank value } × 100%, the results are shown in Table 2.
2 hydration rate test result of table
| Test sample | 0h | 0.5h | 1h | 2h | 4h | 6h |
| 1 ground Folium Camelliae sinensis fermented product of embodiment | 0 | 82.79 | 59.11 | 47.05 | 41.14 | 40.01 |
| 2 ground Folium Camelliae sinensis fermented product of embodiment | 0 | 88.89 | 65.66 | 53.98 | 49.01 | 47.57 |
| 3 ground Folium Camelliae sinensis fermented product of embodiment | 0 | 90.41 | 66.09 | 56.16 | 49.93 | 47.85 |
| 4 ground Folium Camelliae sinensis fermented product of embodiment | 0 | 101.29 | 80.03 | 65.01 | 59.24 | 57.72 |
| 1 ground tea extract of comparative example | 0 | 48.79 | 29.31 | 19.11 | 15.02 | 13.04 |
| 2 ground Folium Camelliae sinensis fermented product of comparative example | 0 | 67.52 | 43.37 | 35.58 | 27.87 | 25.20 |
| 3 ground Folium Camelliae sinensis fermented product of comparative example | 0 | 62.14 | 41.27 | 34.22 | 26.73 | 24.59 |
| 4 ground Folium Camelliae sinensis fermented product of comparative example | 0 | 66.21 | 37.49 | 30.88 | 26.08 | 23.41 |
| 5 ground Folium Camelliae sinensis fermented product of comparative example | 0 | 66.69 | 38.02 | 31.24 | 27.10 | 24.78 |
| 6 ground Folium Camelliae sinensis fermented product of comparative example | 0 | 64.62 | 39.10 | 29.21 | 25.15 | 22.48 |
| Sodium Hyaluronate | 0 | 57.58 | 35.87 | 27.37 | 23.65 | 20.77 |
| Distilled water | 0 | 1.06 | -0.11 | -0.35 | -0.14 | -0.82 |
The above results show that the ground Folium Camelliae sinensis fermented product of 1-4 of the embodiment of the present invention is respectively provided with good performance of keeping humidity.And
After 6 hours, for the moisture content value of skin more than 49, skin hydration rate illustrates system of the present invention more than 40%
Standby ground Folium Camelliae sinensis fermented product has the effect of lasting moisturizing.
Particularly, after the ground Folium Camelliae sinensis fermented product prepared using embodiment 4, moisture is apparently higher than other embodiment 1-
3rd, comparative example 1-6 and Sodium Hyaluronate and distilled water.It can be seen that bacterium is revealed using Sparassis crispa, grifola frondosus, Trichotoma matsutake, kahikatea
The ground Folium Camelliae sinensis fermented product obtained with summer truffle multiple eating bacterium mixed fermentation, the performance of keeping humidity reached are best.
2 clinic trial of experimental example is observed
1) crowd is tested：Age, scaly dry skin, tight and microgroove 200 people of subject were divided into 10 groups at 20-50 Sui.Often
20 people of group.
Laboratory sample：Ground Folium Camelliae sinensis fermented product, the ground tealeaves of the preparation of comparative example 1 that the embodiment of the present application 1-4 is provided is taken to extract
Ground Folium Camelliae sinensis fermented product prepared by object, comparative example 2-6 is added in Essence matrix, and additive amount is the 10% of skin care item gross mass.
Embodiment 1-4 moisturizing essence, comparative example 1-6 moisturizing essence are named as successively accordingly.
3) application method：Above-mentioned test sample is wiped in face after clean face sooner or later daily, adheres to using 2 months.
4) result：User more than 85% represents that after the moisturizing essence 2 months of embodiment 1-4 is used skin is done
Dry, tight and microgroove be improved significantly, wherein user more than 95% represents using the moisturizing essence 2 of embodiment 4
After a month, skin becomes water and moistens smooth exquisiteness；And the effect of comparative example 1-6 moisturizing essence is not as good as 1-4 of the embodiment of the present invention
The effect of moisturizing essence, only 40% user represented after the moisturizing essence 2 months of embodiment 1 is used, dry
Skin makes moderate progress.
5) subject is adhered to using the test sample for preparing of the present invention after 3 months, without occur facial sufferings, redness,
The allergic symptoms such as oedema, inflammation.
In conclusion a kind of ground Folium Camelliae sinensis fermented product using edible mushroom provided by the invention, has good moisture-keeping efficacy,
Scaly dry skin, tight and microgroove problem are can effectively improve, and skin allergy will not be caused.
Claims (10)
1. a kind of ground Folium Camelliae sinensis fermented product using edible mushroom, which is characterized in that make ground tea leaf fermentation using edible mushroom and obtain.
A kind of 2. ground Folium Camelliae sinensis fermented product using edible mushroom according to claim 1, which is characterized in that the edible mushroom
Including Sparassis crispa, grifola frondosus and Trichotoma matsutake.
A kind of 3. ground Folium Camelliae sinensis fermented product using edible mushroom according to claim 2, which is characterized in that the edible mushroom
Further include kahikatea dew bacterium.
A kind of 4. ground Folium Camelliae sinensis fermented product using edible mushroom according to Claims 2 or 3, which is characterized in that the food
Summer truffle is further included with bacterium.
5. the preparation method of the ground Folium Camelliae sinensis fermented product described in claim 1 using edible mushroom, which is characterized in that under including successively
State step：
(1) seed liquor is prepared
Respectively by each fruit body of edible fungi aseptically clip tissue fragment, and be inoculated in slant medium respectively,
Quiescent culture 8-10 days at 25-28 DEG C of temperature；Again aseptically from slant medium picking carry mycelia agar 10-
It 20 pieces, accesses again in sterile slant medium, at 25~28 DEG C, light culture is after 12~15 days, illumination 3~4 days, chooses and turns
The strain that color is very fast and mycelial growth is neat, as parent species, is then seeded in seed culture medium, in 26-28 DEG C of temperature, shaking table
It under the conditions of rotating speed is 150-200r/min, cultivates 5-7 days, respectively obtains the seed liquor of each edible mushroom；
(2) by ground tealeaves Ultramicro-powder essence to 80-100 μm, the water of 2-4 times of quality is added in, is impregnated 1-2 days at 30-40 DEG C, from
The heart obtains ground tealeaves soaked extracting solution；
(3) each edible mushroom seed liquor that step (1) is prepared to gained mixes in advance according to isometric ratio, with the inoculum concentration of 5-10%
It accesses in high density fermentation culture medium 5L fermentation tanks, and the ground tealeaves soaked extracting solution for adding in step (2) starts fermented and cultured, ground
Infusion of tea extracting solution and the mass ratio of edible mushroom seed liquor are 3-6：1, it is 28-30 DEG C in temperature, rotating speed 100-250r/
Min, throughput 0.5-1.5dm3Fermented and cultured 5-10 days under the conditions of/min obtains the mixture of edible fungus fermented ground tealeaves,
Centrifugation, takes supernatant liquid filtering.
(4) carry out being concentrated under reduced pressure into moisture content under conditions of the filtrate that step (3) is obtained by filtration is below 60 DEG C less than 8%,
Then it is freeze-dried, obtained powder as Folium Camelliae sinensis fermented product.
A kind of 6. preparation method of ground Folium Camelliae sinensis fermented product using edible mushroom according to claim 5, which is characterized in that step
Suddenly slant medium described in (1) is PDA culture medium or PDPA synthetic mediums.
A kind of 7. preparation method of ground Folium Camelliae sinensis fermented product using edible mushroom according to claim 5, which is characterized in that step
Suddenly seed culture medium is described in (1)：PH6.0-6.5, based on its total mass fraction 100%, by glucose 1.5-2.5%, egg
White peptone 0.1-0.2%, beef extract 0.1-0.2%, KH2PO40.1-0.15%, MgSO40.1-0.15%, vitaminB10 .01-
0.02%, surplus is water.
A kind of 8. preparation method of ground Folium Camelliae sinensis fermented product using edible mushroom according to claim 5, which is characterized in that step
Suddenly fermentation medium pH 5.5~6.0 described in (3), based on its total mass fraction 100%, by glucose 2-3%, peptone
0.4-0.6%, yeast extract 0.2-0.4%, KH2PO40.15-0.3%, MgSO40.1-0.15%, vitaminB10 .01-
0.02%, surplus is water.
9. a kind of ground Folium Camelliae sinensis fermented product by the use of edible mushroom described in claim 1 is as the application of skin care item moisturizer.
10. a kind of ground Folium Camelliae sinensis fermented product by the use of edible mushroom described in claim 1 is as the application of skin care item moisturizer, special
Sign is that mass percent of the moisture-keeping composition in skin care item is 0.1-20%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711413404.3A CN108066210B (en) | 2017-12-24 | 2017-12-24 | A herba Hyperici Japonici leaf fermented product using domestic fungus, and its preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711413404.3A CN108066210B (en) | 2017-12-24 | 2017-12-24 | A herba Hyperici Japonici leaf fermented product using domestic fungus, and its preparation method and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108066210A true CN108066210A (en) | 2018-05-25 |
| CN108066210B CN108066210B (en) | 2021-03-16 |
Family
ID=62155657
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711413404.3A Active CN108066210B (en) | 2017-12-24 | 2017-12-24 | A herba Hyperici Japonici leaf fermented product using domestic fungus, and its preparation method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108066210B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113215004A (en) * | 2021-06-11 | 2021-08-06 | 山东效峰生物科技股份有限公司 | Method for regulating and controlling balanced growth in mixed culture process of edible and medicinal fungi |
| CN113373059A (en) * | 2021-06-15 | 2021-09-10 | 山东效峰生物科技股份有限公司 | Method for increasing content of adenosine and ergosterol in fungus mixed fermentation liquid |
| CN114668055A (en) * | 2022-04-12 | 2022-06-28 | 广州合和昌茶业科技有限公司 | Auxiliary material composition for tea fermentation and fermentation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1891027A (en) * | 2002-07-18 | 2007-01-10 | 福建省农业科学院土壤肥料研究所 | Process for producing Grifolar Frondosus fermented tea |
| CN104872664A (en) * | 2015-05-26 | 2015-09-02 | 成都易创思生物科技有限公司 | Method for manufacturing health product by utilizing cordyceps grown in Nagqu Prefecture |
| CN104982571A (en) * | 2015-06-17 | 2015-10-21 | 杜超峰 | Endophyte fermented tea and preparing method thereof |
| CN105961666A (en) * | 2016-06-12 | 2016-09-28 | 孙超 | Tricholoma matsutake hypha fermented tea and processing method thereof |
| CN106074263A (en) * | 2016-07-05 | 2016-11-09 | 上海相宜本草化妆品股份有限公司 | A kind of book on Chinese herbal medicine extract and the application in cosmetics thereof |
-
2017
- 2017-12-24 CN CN201711413404.3A patent/CN108066210B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1891027A (en) * | 2002-07-18 | 2007-01-10 | 福建省农业科学院土壤肥料研究所 | Process for producing Grifolar Frondosus fermented tea |
| CN104872664A (en) * | 2015-05-26 | 2015-09-02 | 成都易创思生物科技有限公司 | Method for manufacturing health product by utilizing cordyceps grown in Nagqu Prefecture |
| CN104982571A (en) * | 2015-06-17 | 2015-10-21 | 杜超峰 | Endophyte fermented tea and preparing method thereof |
| CN105961666A (en) * | 2016-06-12 | 2016-09-28 | 孙超 | Tricholoma matsutake hypha fermented tea and processing method thereof |
| CN106074263A (en) * | 2016-07-05 | 2016-11-09 | 上海相宜本草化妆品股份有限公司 | A kind of book on Chinese herbal medicine extract and the application in cosmetics thereof |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113215004A (en) * | 2021-06-11 | 2021-08-06 | 山东效峰生物科技股份有限公司 | Method for regulating and controlling balanced growth in mixed culture process of edible and medicinal fungi |
| CN113373059A (en) * | 2021-06-15 | 2021-09-10 | 山东效峰生物科技股份有限公司 | Method for increasing content of adenosine and ergosterol in fungus mixed fermentation liquid |
| CN114668055A (en) * | 2022-04-12 | 2022-06-28 | 广州合和昌茶业科技有限公司 | Auxiliary material composition for tea fermentation and fermentation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108066210B (en) | 2021-03-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR101353054B1 (en) | fermented product and manufacturing method thereof, food composition cosmetic and compositon | |
| KR101566320B1 (en) | Cosmetic composition comprising an Impatiens balsamina L, Maca, Rosa multiflora Thunberg, Eryobotrya japonica and Pachyrrhizus erosus Complex-fermented extract | |
| CN108836919B (en) | Moisturizing cosmetic composition and preparation method and application thereof | |
| CN108066210A (en) | It is a kind of to utilize ground Folium Camelliae sinensis fermented product of edible mushroom and preparation method and application | |
| CN109897799A (en) | One plant of production γ-aminobutyric acid lactic acid bacteria strains and its screening technique and the preparation method rich in γ-aminobutyric acid mesona Yoghourt | |
| CN105726398A (en) | Tremella ferment and prickly pear ferment face cream and preparation method thereof | |
| CN106619379B (en) | A kind of composition of the product containing plant fermentation and its preparation method and application | |
| CN109939059B (en) | Rice germ five-bacterium fermentation slow-release cosmetic and preparation method and application thereof | |
| KR102403309B1 (en) | Natural mixture for improving health and functional cosmetic agent including the same for preventing hair loss | |
| CN113842341B (en) | Roselle fermentation product and preparation method and application thereof | |
| KR101985381B1 (en) | Cosmetic composition for exfoliating skin keratin comprising fruit fermentation complex made by fermenting grape, orange, apple, lemon and lime | |
| KR101663379B1 (en) | MANUFACTURE OF FERMENTED Allium hookeri FROM YEASTS AND NATURAL ENZYME AND PREPARATION OF COMBINED BEVERAGE FOR QUENCHING THIRST | |
| CN102511296A (en) | SOD (superoxide dismutase)-enriched strawberries and production method thereof | |
| KR20090129277A (en) | Laver containing green tea and manufacturing method thereof | |
| CN103122294B (en) | Wine containing SOD (Superoxide Dismutase) component and preparation method thereof | |
| CN101125019A (en) | Pholiota adiposa mycelium fermentation vinegar beverage and preparation method | |
| CN115040442A (en) | Anti-inflammatory toning lotion containing black truffle extract fermentation product and preparation method thereof | |
| CN113413335B (en) | Extract containing algae essence, preparation method thereof and prepared moisturizing emulsion | |
| KR101761771B1 (en) | Method for fermenting Broussonetia plant or the extract thereof, the fermented extracts thereby and the cosmetic composition containing the same | |
| KR102092007B1 (en) | Cosmetic composition containing fermented solution of extract of Nypa fruiticans Wurmb, Fruit | |
| CN109646381A (en) | A kind of moisturizing lotion and preparation method thereof added with plant extracts | |
| CN110680764A (en) | Essence containing agaricus bisporus extract and preparation method thereof | |
| KR20010087699A (en) | The cultivation method of Cordyceps sinensis et al. having a high concentration of gamma-aminobutyric acid | |
| KR20130073059A (en) | Composition containing fermented extract of epicarp of ginkgo biloba for natural preservative | |
| CN107496273A (en) | Camellia oleifera fruit mildy wash and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |