CN113413335B - Extract containing algae essence, preparation method thereof and prepared moisturizing emulsion - Google Patents
Extract containing algae essence, preparation method thereof and prepared moisturizing emulsion Download PDFInfo
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Abstract
The invention provides an extract containing algae essences, a preparation method of the extract and a prepared moisturizing emulsion, and belongs to the technical field of cosmetics. The moisturizing emulsion comprises the following components in parts by mass: 20200.5-1.2% of carbomer resin, 0.1-1% of dipotassium glycyrrhizinate, 0.2-0.5% of water-soluble vitamin E, 4-10% of extract containing algae essence, 0.5-1.5% of shea butter, 2-4% of glycerol, 1-2% of butanediol, 0.02-0.05% of disodium EDTA, 0.01-0.03% of phenoxyethanol and the balance of purified water. The preparation method is simple, the seaweed is recycled and processed into useful cosmetic raw materials, and the cosmetic raw materials have good effects of resisting oxidation and aging, enhancing nutrition, preserving moisture and the like, so that the prepared moisturizing emulsion has a good using effect, and tests show that the moisturizing emulsion has the characteristic of low sensitivity.
Description
Technical Field
The invention relates to the technical field of cosmetics, and particularly relates to an extract containing algae essences, a preparation method of the extract and a prepared moisturizing emulsion.
Background
The moisture retention is the most basic and important requirement of cosmetics, as long as the skin care products are skin care products, the moisture retention cannot be avoided, other functions such as whitening, wrinkle resistance, oxidation resistance and the like are further summed and developed on the basis, and even oil control cosmetics cannot be kept moisture due to the fact that a large amount of moisture is consumed by skin oil metabolism.
The skin has the functions of barrier protection, hydration, moisture retention, sensory stimulation, body temperature regulation, secretion and excretion, absorption and metabolism and the like, and the skin which is most closely involved in the cosmetic is the epidermis, particularly the horny layer, and the horny cells are sandwiched by lipid to form a unique sandwich-like structure through the observation of a submicron structure. The stratum corneum acts like a "brick wall", the keratinocytes are "bricks", and the intercellular lipids are "mud" that acts as an adhesive, tightly linking the keratinocytes, normalizing the skin barrier, and really maintaining the skin. Intercellular lipids generally contain 40% ceramide, 25% cholesterol, 25% free fatty acids, and others. The stratum corneum contains some metabolites which form natural moisturizing factors, namely 40% of amino acid, 12% of lactate, 12% of pyrrolidone carboxylic acid, 7% of urea and the like; sebum is secreted by sebaceous glands, and can form milky sebaceous membrane after being mixed with sweat on the surface of skin, so that the sebum can maintain the healthy and micro-ecology of the skin and inhibit the growth and reproduction of bacteria and fungi besides softening the skin, and the sebum comprises the following components: triglyceride 57.5%, wax 26%, squalene 12% and others.
Traditional moisturizing method generally comprises adding ceramide, cholesterol, free fatty acid, other oil and fat, glycerol, butanediol, amino acid, lactic acid, urea, etc. from the aspects of intercellular lipid, natural moisturizing factor, etc. Intercellular lipid is oil-soluble, and is difficult to be added into some water-based transparent products such as moisturizing water, moisturizing essence and the like. Amino acid, lactic acid, urea and the like belong to micromolecule humectants which are easy to lose and cannot achieve long-acting moisture preservation. Glycerin, sorbitol, butanediol and the like are locked and retain moisture through hydroxyl groups with high molecular formulas, and can be influenced by the environmental humidity and have positive influence in a humid environment; in a very dry environment, the effect is relatively negative.
Codiumfragile (sur.) Hariot, commonly known as cochineal and rat tail, belongs to the genus Pinus of Pinaceae of Capsosiphon of Chlorophyceae of Chlorophyta of Rhodophyta, and is a worldwide warm temperate seaweed. With the discovery of remarkable biological activity of the codium algae polysaccharide, the structural research is also deepened, and the codium algae aqueous extract is rich in alpha (1 → 4) -D-glucan, beta (1 → 4) -D-mannan, highly sulfated and pyruvylated beta (1 → 3) -D-galactan and sulfated beta (1 → 3) -L-araban. Bryopsis hypnoides is a polynuclear unicellular green alga and also contains abundant algal polysaccharides.
In recent years, complex carbohydrates have gained increased attention because they are less dependent on air humidity. Algae contains abundant saccharides, is natural and efficient, is easy to be absorbed by skin, is more and more accepted by consumers, has the validity of marine plant components on human skin accepted by world authorities, and is widely applied to the field of cosmetics. Currently, the most widely used non-algal genus morus is among the marine plant components. The algae grows in seawater medium with high salt, high pressure, low temperature, low nutrition, no illumination and extremely complex chemical components, the ecological environment, metabolites and metabolic characteristics of the algae are greatly different from those of land plants, and the algae are concentrated in that the algae contain biological active substances and metabolites with special structures in organisms. The algae contains abundant carbohydrates, proteins, lipids, trace elements, minerals, vitamins and the like, and the active matters can be applied to novel functional cosmetics and have wide market prospects.
Patent CN105777933A discloses a preparation method of algal polysaccharide and an application of algal polysaccharide in cosmetics, the algal polysaccharide is extracted and applied to the cosmetics to achieve the effects of moistening, reducing wrinkles and delaying senescence, however, the skin-moistening cream containing algal polysaccharide prepared by the patent can cause certain skin sensitivity through tests, and is not suitable for wide application.
Disclosure of Invention
The invention aims to provide an extract containing algae essence, a preparation method thereof and a prepared moisturizing emulsion, which are used for recycling algae and processing the algae into useful cosmetic raw materials, have good effects of resisting oxidation and aging, enhancing nutrition, moisturizing and the like, so that the prepared moisturizing emulsion has an excellent using effect, and tests show that the moisturizing emulsion has the characteristic of low allergy.
The technical scheme of the invention is realized as follows:
the invention provides a preparation method of an extract containing algae essence, which comprises the following steps:
s1, respectively cleaning and mechanically crushing the codium spiniferum and the bryophyte, adding distilled water, stirring and mixing, carrying out microwave heating extraction, filtering, and carrying out reduced pressure concentration to obtain an algae extract;
s2, adding an algae extract into a yeast fermentation culture medium, sterilizing, inoculating the saccharomyces cerevisiae and the candida utilis after seed culture according to the inoculation amounts of 2-3% and 1-4%, fermenting and culturing for 3-5 days, filtering, taking fermentation liquor, adding absolute ethyl alcohol, filtering, and washing solids with ethyl alcohol to obtain algal polysaccharides;
s3, dissolving algal polysaccharides in distilled water, and filtering to obtain an extract containing algae essence.
As a further improvement of the invention, the mass ratio of the codium spiniferum to the bryophyte is (2-5): 2, preferably 3: 2.
As a further improvement of the invention, the relative density of the algae extract is 1.3-1.7.
As a further improvement of the invention, the mass ratio of the yeast fermentation medium to the algae extract is 10: (9-15).
As a further improvement of the invention, the microwave is heated to 45-60 ℃, and the microwave power is 1000-1200W.
As a further improvement of the invention, the fermentation culture conditions in the step S2 are that the temperature is 28-34 ℃, the rotation speed is 100-150r/min, and the ventilation quantity is 2-5 VVm.
As a further improvement of the invention, the volume ratio of the fermentation liquor to the absolute ethyl alcohol is 1: (1-3).
The invention further provides the extract containing the algae essence, which is prepared by the preparation method, wherein the polysaccharide content in the extract containing the algae essence is 40-60%.
The invention further protects the moisturizing emulsion which contains 4-10wt% of the extract containing the algae essence.
As a further improvement of the invention, the feed additive comprises the following components in percentage by mass: 20200.5-1.2% of carbomer resin, 0.1-1% of dipotassium glycyrrhizinate, 0.2-0.5% of water-soluble vitamin E, 4-10% of extract containing algae essence, 0.5-1.5% of shea butter, 2-4% of glycerol, 1-2% of butanediol, 0.02-0.05% of disodium EDTA, 0.01-0.03% of phenoxyethanol and the balance of purified water.
The invention has the following beneficial effects: the invention further ferments the polysaccharide extracted from the codium spinulosum and the bryophyte by a compound yeast fermentation mode to obtain polysaccharide with rich nutrition, such as alpha glucan, beta glucomannan, beta mannan, beta galactan, beta araban and the like, and oligosaccharide and monosaccharide compounds obtained by fermentation, and micromolecule compounds obtained by saccharide fermentation, the extract obtained by mixing the substances has excellent moisturizing effect, water can be introduced into a skin crystalline structure by combining with the moisture in the air to play a role in moisturizing, and meanwhile, a layer of protective film layer can be formed on the surface of the skin by polysaccharide substances to keep the moisture of the skin from being dissipated, so that the effect of water locking is played;
according to the invention, composite yeast is adopted for fermentation, saccharomyces cerevisiae and candida utilis are mixed for fermentation, and the two are fermented in a synergistic manner, so that the algae extract is fermented to generate a compound of polysaccharide, oligosaccharide and monosaccharide with small molecular weight, and further fermented to generate small molecules such as some short peptides, vitamins and the like, so that the nutrition of the algae-containing essence extract is further enhanced, and the composite yeast has good effects of aging resistance, oxidation resistance and the like;
the water-soluble vitamin E can protect cell membranes from being damaged by free radicals, so that the water-soluble vitamin E can help to repair and consolidate natural barriers of the skin, lock the moisture of the skin and provide deep nourishment; after the water-soluble vitamin E and the prepared algae-containing essence extract are compounded and mixed, the moisturizing performance of the moisturizing emulsion is further improved, and the emulsion has good oxidation resistance and aging resistance, so that the moisturizing and skin-care performance of the emulsion is remarkably improved;
the preparation method is simple, the seaweed is recycled and processed into useful cosmetic raw materials, and the cosmetic raw materials have good effects of resisting oxidation and aging, enhancing nutrition, preserving moisture and the like, so that the prepared moisturizing emulsion has a good using effect, and tests show that the moisturizing emulsion has the characteristic of low sensitivity.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.
Codium spinulosum, produced in the yellow sea area, purchased from Rongcheng Haihishi food Co., Ltd; the moss-feather algae are collected from the intertidal zone of the sink spring of Qingdao.
Example 1
The embodiment provides a preparation method of an extract containing algae essence, which comprises the following steps:
s1, respectively cleaning 20g of Codium spinulosum and 20g of Codium bryonioides, mechanically pulverizing, adding 500mL of distilled water, stirring and mixing, performing microwave heating extraction, performing microwave heating to 45 ℃, performing microwave power of 1000W, filtering, and performing reduced pressure concentration to obtain an algae extract, wherein the relative density is 1.3;
s2, adding 90g of algae extract into 100g of yeast fermentation medium, sterilizing at 105 ℃ for 20min, respectively inoculating 2% and 1% of inoculum size to the saccharomyces cerevisiae and the candida utilis which are subjected to seed culture, performing fermentation culture for 3 days under the conditions that the temperature is 28 ℃, the rotating speed is 100r/min, the ventilation quantity is 2VVm, filtering, taking fermentation liquor, adding absolute ethyl alcohol, wherein the volume ratio of the fermentation liquor to the absolute ethyl alcohol is 1: 1, filtering, and washing a solid with ethanol to obtain algal polysaccharide;
the yeast fermentation medium comprises the following components: 10g/L glucose, 1g/L sucrose, 8g/L, KCl 3g/L peptone and 2g/L, K ammonium nitrate 2 HP0 4 0.1g/L、MgSO 4 0.2g/L and pH 5.5;
s3, dissolving 10g of algal polysaccharide in 10mL of distilled water, and filtering to obtain an extract containing algae essence, wherein the polysaccharide content is 48% according to a phenol-sulfuric acid method.
Example 2
The embodiment provides a preparation method of an extract containing algae essence, which comprises the following steps:
s1, respectively cleaning 50g of codium spiniferum and 20g of bryophyte, mechanically crushing, adding 500mL of distilled water, stirring and mixing, carrying out microwave heating extraction, heating to 60 ℃ by microwave, controlling the microwave power at 1200W, filtering, and carrying out reduced pressure concentration to obtain an algae extract, wherein the relative density is 1.7;
s2, adding 150g of algae extract into 100g of yeast fermentation medium, sterilizing at 105 ℃ for 20min, respectively inoculating 3% and 4% of inoculum size to the saccharomyces cerevisiae and the candida utilis which are subjected to seed culture, fermenting and culturing for 5 days under the conditions that the temperature is 34 ℃, the rotating speed is 150r/min and the ventilation volume is 5VVm, filtering, taking fermentation liquor, adding absolute ethyl alcohol, wherein the volume ratio of the fermentation liquor to the absolute ethyl alcohol is 1: 3, filtering, and washing the solid with ethanol to obtain algal polysaccharide;
the yeast fermentation medium comprises the following components: 14g/L glucose, 5g/L sucrose, 10g/L, KCl 7g/L peptone and 5g/L, K ammonium nitrate 2 HP0 4 2g/L、MgSO 4 0.4g/L and pH 5.7;
s3, dissolving 10g of algal polysaccharide in 10mL of distilled water, and filtering to obtain an extract containing the algae essence, wherein the polysaccharide content is 51% according to a phenol-sulfuric acid method test.
Example 3
The embodiment provides a preparation method of an extract containing algae essence, which comprises the following steps:
s1, respectively cleaning 35g of codium spiniferum and 20g of bryophyte pinnata, mechanically crushing, adding 500mL of distilled water, stirring and mixing, carrying out microwave heating extraction, carrying out microwave heating to 55 ℃, wherein the microwave power is 1100W, filtering, and carrying out reduced pressure concentration to obtain an algae extract, wherein the relative density is 1.5;
s2, adding 120g of algae extract into 100g of yeast fermentation medium, sterilizing at 105 ℃ for 20min, respectively inoculating 2.5% and 3% of inoculation amount to the saccharomyces cerevisiae and the candida utilis which are subjected to seed culture, performing fermentation culture for 4 days under the conditions that the temperature is 32 ℃, the rotating speed is 125r/min and the ventilation volume is 3.5VVm, filtering, taking fermentation liquor, adding absolute ethyl alcohol, wherein the volume ratio of the fermentation liquor to the absolute ethyl alcohol is 1: 2, filtering, and washing the solid with ethanol to obtain algal polysaccharide;
the yeast fermentation medium comprises the following components: 12g/L glucose, 5g/L sucrose, 9g/L, KCl 5g/L peptone and 3.5g/L, KH ammonium nitrate 2 P0 4 1g/L、MgSO 4 ﹒7H 2 O is 0.3g/L, and the pH value is 5.6;
s3, dissolving 10g of algal polysaccharide in 10mL of distilled water, and filtering to obtain an extract containing the algae essence, wherein the polysaccharide content is 55% according to a phenol-sulfuric acid method.
Comparative example 1
In comparison with example 3, no P.spinosa was added, and the other conditions were not changed.
The method comprises the following steps:
s1, respectively cleaning 55g of bryophyte feather algae, mechanically crushing, adding 500mL of distilled water, stirring and mixing, carrying out microwave heating extraction, carrying out microwave heating to 55 ℃, wherein the microwave power is 1100W, filtering, and carrying out reduced pressure concentration to obtain an algae extract, wherein the relative density is 1.5;
s2, adding 120g of algae extract into 100g of yeast fermentation medium, sterilizing at 105 ℃ for 20min, respectively inoculating 2.5% and 3% of inoculation amount to the saccharomyces cerevisiae and the candida utilis which are subjected to seed culture, performing fermentation culture for 4 days under the conditions that the temperature is 32 ℃, the rotating speed is 125r/min and the ventilation volume is 3.5VVm, filtering, taking fermentation liquor, adding absolute ethyl alcohol, wherein the volume ratio of the fermentation liquor to the absolute ethyl alcohol is 1: 2, filtering, and washing the solid with ethanol to obtain algal polysaccharide;
the yeast fermentation medium comprises the following components: 12g/L glucose, 5g/L sucrose, 9g/L, KCl 5g/L peptone and 3.5g/L, KH ammonium nitrate 2 P0 4 1g/L、MgSO 4 ﹒7H 2 O is 0.3g/L, and the pH value is 5.6;
s3, dissolving 10g of algal polysaccharide in 10mL of distilled water, filtering to obtain an extract containing algae essence, wherein the polysaccharide content is 42% according to a phenol-sulfuric acid method.
Comparative example 2
In comparison with example 3, no bryophyte was added, and other conditions were unchanged.
The method comprises the following steps:
s1, cleaning 55g of Codium spinulosum, mechanically pulverizing, adding 500mL of distilled water, stirring and mixing, performing microwave heating extraction, heating the microwave to 55 ℃, controlling the microwave power to be 1100W, filtering, and concentrating under reduced pressure to obtain an algae extract with the relative density of 1.5;
s2, adding 120g of algae extract into 100g of yeast fermentation medium, sterilizing at 105 ℃ for 20min, respectively inoculating 2.5% and 3% of inoculation amount to the saccharomyces cerevisiae and the candida utilis which are subjected to seed culture, performing fermentation culture for 4 days under the conditions that the temperature is 32 ℃, the rotating speed is 125r/min and the ventilation volume is 3.5VVm, filtering, taking fermentation liquor, adding absolute ethyl alcohol, wherein the volume ratio of the fermentation liquor to the absolute ethyl alcohol is 1: 2, filtering, and washing the solid with ethanol to obtain algal polysaccharide;
the yeast fermentation medium comprises the following components: 12g/L glucose, 5g/L sucrose, 9g/L, KCl 5g/L peptone and 3.5g/L, KH ammonium nitrate 2 P0 4 1g/L、MgSO 4 ﹒7H 2 O is 0.3g/L, and the pH value is 5.6;
s3, dissolving 10g of algal polysaccharide in 10mL of distilled water, filtering to obtain an extract containing the algae essence, wherein the polysaccharide content is 40% according to a phenol-sulfuric acid method.
Comparative example 3
Compared with example 3, no saccharomyces cerevisiae was inoculated, and other conditions were not changed.
The embodiment provides a preparation method of an extract containing algae essence, which comprises the following steps:
s1, respectively cleaning 35g of codium spiniferum and 20g of bryophyte, mechanically crushing, adding 500mL of distilled water, stirring and mixing, carrying out microwave heating extraction, heating to 55 ℃ by microwave with the microwave power of 1100W, filtering, and carrying out reduced pressure concentration to obtain an algae extract with the relative density of 1.5;
s2, adding 120g of algae extract into 100g of yeast fermentation medium, sterilizing at 105 ℃ for 20min, inoculating the candida utilis yeast cultured by seeds according to the inoculum size of 5.5%, fermenting and culturing for 4 days under the conditions that the temperature is 32 ℃, the rotating speed is 125r/min, the ventilation volume is 3.5VVm, filtering, taking fermentation liquor, adding absolute ethyl alcohol into the fermentation liquor, wherein the volume ratio of the fermentation liquor to the absolute ethyl alcohol is 1: 2, filtering, and washing the solid with ethanol to obtain algal polysaccharide;
the yeast fermentation medium comprises the following components: 12g/L glucose, 5g/L sucrose, 9g/L, KCl 5g/L peptone and 3.5g/L, KH ammonium nitrate 2 P0 4 1g/L、MgSO 4 ﹒7H 2 O is 0.3g/L, and the pH value is 5.6;
s3, dissolving 10g of algal polysaccharide in 10mL of distilled water, and filtering to obtain an extract containing the algae essence, wherein the polysaccharide content is 45% according to a phenol-sulfuric acid method test.
Comparative example 4
Compared with example 3, the candida utilis is not inoculated, and other conditions are not changed.
The embodiment provides a preparation method of an extract containing algae essence, which comprises the following steps:
s1, respectively cleaning 35g of codium spiniferum and 20g of bryophyte, mechanically crushing, adding 500mL of distilled water, stirring and mixing, carrying out microwave heating extraction, heating to 55 ℃ by microwave with the microwave power of 1100W, filtering, and carrying out reduced pressure concentration to obtain an algae extract with the relative density of 1.5;
s2, adding 120g of algae extract into 100g of yeast fermentation medium, sterilizing at 105 ℃ for 20min, inoculating the saccharomyces cerevisiae after seed culture according to the inoculation amount of 5.5%, fermenting and culturing for 4 days under the conditions that the temperature is 32 ℃, the rotating speed is 125r/min, the ventilation quantity is 3.5VVm, filtering, taking fermentation liquor, adding absolute ethyl alcohol, wherein the volume ratio of the fermentation liquor to the absolute ethyl alcohol is 1: 2, filtering, and washing the solid with ethanol to obtain algal polysaccharide;
the yeast fermentation medium comprises the following components: 12g/L glucose, 5g/L sucrose, 9g/L, KCl 5g/L peptone and 3.5g/L, KH ammonium nitrate 2 P0 4 1g/L、MgSO 4 ﹒7H 2 O is 0.3g/L, and the pH value is 5.6;
s3, dissolving 10g of algal polysaccharide in 10mL of distilled water, and filtering to obtain an extract containing the algae essence, wherein the polysaccharide content is 47% according to a phenol-sulfuric acid method test.
Example 4 moisturizing emulsion
The composite material comprises the following components in percentage by mass: 20200.5% of carbomer resin, 0.1% of dipotassium glycyrrhizinate, 0.2% of water-soluble vitamin E, 4% of the extract containing the algae essence prepared in example 1, 0.5% of shea butter, 2% of glycerol, 1% of butanediol, 0.02% of disodium EDTA, 0.01% of phenoxyethanol and the balance of purified water.
The preparation method comprises the following steps:
1) adding purified water into water phase pot, slowly adding butanediol, glycerol, dipotassium glycyrrhizinate, water-soluble vitamin E, extract containing algae essence and EDTA disodium under stirring, heating to 77 deg.C, stirring, and keeping the temperature for 4 min;
2) adding carbomer resin 2020 and shea butter into oil phase pan, heating to 77 deg.C, stirring, and keeping the temperature for 7 min;
3) filtering the materials in the water phase and oil phase pot, sequentially pumping into a vacuum emulsifying pot, homogenizing for 7min, slowly cooling to 42 deg.C, adding phenoxyethanol, stirring, discharging after detection, and bottling.
Example 5
Compared with the example 4, the formula and the components are different, and the preparation method is consistent.
The composite material comprises the following components in percentage by mass: 20201.2% of carbomer resin, 1% of dipotassium glycyrrhizinate, 0.5% of water-soluble vitamin E, 10% of the algae essence-containing extract prepared in example 2, 1.5% of shea butter, 4% of glycerol, 2% of butanediol, 0.05% of disodium EDTA, 0.03% of phenoxyethanol and the balance of purified water.
Example 6
Compared with the example 4, the formula and the components are different, and the preparation method is consistent.
The composite material comprises the following components in percentage by mass: carbomer resin 20201%, dipotassium glycyrrhizinate 0.5%, water-soluble vitamin E0.35%, the extract containing the algae essence prepared in example 3 7%, shea butter 1%, glycerin 3%, butanediol 1.5%, disodium EDTA 0.035%, phenoxyethanol 0.02%, and the balance of purified water.
Comparative example 5
Compared with example 6, the extract containing the algae essence prepared in example 3 was not added, and other conditions were not changed.
The composite material comprises the following components in percentage by mass: carbomer resin 20201%, dipotassium glycyrrhizinate 0.5%, water-soluble vitamin E7.35%, shea butter 1%, glycerol 3%, butanediol 1.5%, EDTA disodium 0.035%, phenoxyethanol 0.02%, and the balance of purified water.
Comparative example 6
Compared with example 6, no water-soluble vitamin E was added, and other conditions were not changed.
The composite material comprises the following components in percentage by mass: carbomer resin 20201%, dipotassium glycyrrhizinate 0.5%, the extract containing the algae essence prepared in example 3 7.35%, shea butter 1%, glycerin 3%, butanediol 1.5%, disodium EDTA 0.035%, phenoxyethanol 0.02%, and the balance of purified water.
Comparative example 7
Compared with example 6, the extract containing the algae essence prepared in example 3 was replaced with the extract containing the algae essence prepared in comparative example 1, and other conditions were not changed.
Comparative example 8
Compared with example 6, the extract containing algae essence prepared in example 3 was replaced by the extract containing algae essence prepared in comparative example 2, and other conditions were not changed.
Comparative example 9
Compared with example 6, the extract containing the algae essence prepared in example 3 was replaced by the extract containing the algae essence prepared in comparative example 3, and other conditions were not changed.
Comparative example 10
Compared with example 6, the extract containing the algae essence prepared in example 3 was replaced by the extract containing the algae essence prepared in comparative example 4, and other conditions were not changed.
Test example 1 cytotoxicity test:
grouping experiments: the extracts containing the algae essences prepared in examples 1 to 3 and comparative examples 1 to 4 were dissolved in serum-free DMEM medium, and 3 replicates were set for each sample using the serum-free DMEM medium as a negative control group.
MTT detection: well-grown L929 cells were adjusted to a concentration of 1X 10 5 Per mL, 100. mu.L/well into 96-well plates, 5% CO at 37 ℃ 2 And culturing in serum-free DMEM culture solution for 24 hours in the environment. After adherent growth, the culture solution is discarded, 100 mu L of sample solutions with different mass fractions are added into each hole according to the experimental grouping scheme, and the negative control group continues serum-free DMEM culture for 48 h. Adding 20 mu L of 5mg/mL MTT solution into each hole of the cell culture plate, and placing the cell culture plate in an incubator to be incubated in a dark placeAnd (5) breeding for 4 h. The supernatant was aspirated off and 100. mu.L DMSO was added to each well. The cell culture plate was wrapped with aluminum foil paper, placed in a shaker and shaken at low speed for 20min, and the absorbance was measured at a wavelength of 570 nm. The relative survival rate (. beta.) of the other groups of cells was calculated as follows. The results are shown in Table 1.
β＝A n /A 0
In the formula, A n Absorbance for experimental group, A 0 Absorbance of negative control group.
TABLE 1 Effect of cell survival in different groups
According to the toxicity grading evaluation method described in ISO 10993-5:2009, the cell survival rate is more than 70%, and the cells are considered to be non-toxic.
Test example 2 Patch test
300 volunteers meeting the test requirements at the age of 20-50 are selected as test subjects, the test subjects are randomly divided into 8 groups, 30 patients are selected in each group, the potential possibility of adverse reactions caused by the existence of human bodies is determined according to skin closed patch test examples 4-6 and comparative examples 5-10 of a human body patch test in cosmetic safety technical specification and the skin cream containing algal polysaccharide prepared in example 2 in patent CN105777933A, the results are interpreted according to the interpretation standards in the following table 2, and the statistical interpretation results are shown in the following table 3.
TABLE 2
TABLE 3
The patch test showed that examples 4 to 6 of the present invention and comparative examples 6 to 10 had low sensitivity. The comparative example 5 has no added extract containing algae essence and has slight sensitivity, and thus the extract containing algae essence prepared by the invention has obvious effects of relieving skin and reducing skin sensitivity.
The skin moistening cream containing algal polysaccharide prepared in patent CN105777933A produces obvious allergic reaction in a patch test, because the seaweed extract which is not fermented has certain skin irritation, and part of people can not adapt to part of substances in seaweed, so the allergic reaction is produced.
According to the invention, the extract containing the algae essence is fermented by the composite bacteria, and most substances of the obtained polysaccharide extract containing the algae are decomposed into substances with smaller molecular weight, and are easily absorbed and metabolized by skin, so that anaphylactic reaction is not generated.
Test example 3 moisture retention test
The moisturizing emulsions prepared in examples 4 to 6 and comparative examples 5 to 10, and the moisturizing cream containing algal polysaccharide prepared in example 2 of patent CN105777933A were tested by selecting 20 female consumers between 25-48 years of age, and using the forehead as the test site and a fixed area of 2 cm square as the test area on the forehead of the face. The Moisture content of the skin was measured on the forehead skin of 20 female consumers between 18 and 50 years of age in each group using a CorneometerCM820 skin Moisture meter in an indoor environment at 20 ℃ and 50% relative humidity, and the results were expressed as a set Moisture Measurement Value (MMV), and the final MMV Value was expressed as an average Value to evaluate the moisturizing performance of the product, and the results are shown in Table 4.
Wherein: MMV 1-MMV value of skin before applying moisturizing sunscreen lotion, MMV 2-MMV value of skin within 1 minute after applying moisturizing sunscreen lotion, MMV 3-MMV value of skin after 1 hour after applying moisturizing sunscreen lotion; the water supplement rate is MMV2/MMV1 multiplied by 100 percent; moisture retention rate is MMV3/MMV2 x 100%
TABLE 4
As can be seen from the table above, the moisturizing emulsion prepared by the invention has good moisturizing effect.
Compared with the example 6, the comparative examples 5 and 6 have the advantages that the water replenishing and moisturizing effects are obviously reduced without adding the extract containing the algae essence or the water-soluble vitamin E, so that the extract containing the algae essence and the water-soluble vitamin E have obvious promotion effects on the water replenishing and moisturizing of the skin, and the extract containing the algae essence and the water-soluble vitamin E have synergistic effects. The extract containing the algae essence contains rich-nutrient polysaccharides, such as alpha glucan, beta glucomannan, beta mannan, beta galactan, beta araban and the like, a compound of oligosaccharide and monosaccharide obtained by fermentation, and small molecular compounds obtained by fermentation of saccharides, and the extract obtained by mixing the substances has excellent moisturizing effect, can introduce water into a skin crystalline structure by combining moisture in the air to play a role in moisturizing, and simultaneously, the polysaccharide substance can form a protective film layer on the surface of the skin to keep the moisture of the skin from being dissipated so as to play a role in retaining the moisture of the skin; the water-soluble vitamin E can protect cell membranes from being damaged by free radicals, so that the water-soluble vitamin E can help to repair and consolidate natural barriers of skin, lock moisture of the skin and provide deep nutrition; after the water-soluble vitamin E and the prepared algae-containing essence extract are compounded and mixed, the moisturizing performance of the moisturizing emulsion is further improved, and the emulsion has good oxidation resistance and aging resistance, so that the moisturizing and skin-care performance of the emulsion is remarkably improved;
comparative example 7 and comparative example 8 compared with example 6, in which the extract containing the essence of algae was prepared without adding codium spinulosum or bryophyte, respectively, having reduced moisturizing properties, the codium spinulosum contains abundant polysaccharides including alpha glucan, beta mannan, beta galactan, and beta arabinan; the beta glucomannan contained in the bryophyte can lead water to a crystalline structure of the skin by combining with the water in the air to play a role in replenishing water, and the polysaccharide can also form a layer of natural barrier on the surface, so that the high-efficiency water-locking effect is achieved, and the polysaccharides with the abundant species interact with each other to play a role in synergy.
Compared with the example 6, the comparative examples 9 and 10 have the advantages that the extract containing the algae essence is prepared without adding saccharomyces cerevisiae or candida utilis, the water replenishing and moisturizing effects are reduced to a certain degree, and the composite saccharomycetes fermentation is more beneficial to producing various beneficial factors and has a good moisturizing effect.
The seaweed skin care product which is not subjected to fermentation treatment has better moisturizing performance compared with moisturizing emulsion prepared from the seaweed essence extract which is subjected to fermentation treatment, the polysaccharide extracted from the codium spiniferum and the bryophyte is further fermented in a composite yeast fermentation mode to obtain polysaccharide rich in nutrition, such as alpha glucan, beta mannan, beta galactan, beta arabinan and the like, a compound of oligosaccharide and monosaccharide obtained by fermentation, and micromolecule compounds obtained by saccharide fermentation, the extract obtained by mixing the substances has excellent moisturizing effect, water can be introduced into a crystalline structure of the skin by combining water in the air to play a role in moisturizing, and meanwhile, a layer of a protecting film layer can be formed on the surface of the skin by the polysaccharide substance, the moisture of the skin is kept from being dissipated, and the effect of locking water is achieved.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (8)
1. A preparation method of an extract containing algae essence is characterized by comprising the following steps:
s1, respectively cleaning and mechanically crushing the codium spiniferum and the bryophyte, adding distilled water, stirring and mixing, carrying out microwave heating extraction, filtering, and carrying out reduced pressure concentration to obtain an algae extract;
s2, adding an algae extract into a yeast fermentation culture medium, sterilizing, inoculating the saccharomyces cerevisiae and the candida utilis after seed culture according to the inoculation amounts of 2-3% and 1-4%, fermenting and culturing for 3-5 days, filtering, taking fermentation liquor, adding absolute ethyl alcohol, filtering, and washing solids with ethyl alcohol to obtain algal polysaccharides;
s3, dissolving algal polysaccharide in distilled water, and filtering to obtain an extract containing algae essence;
the mass ratio of the codium spiniferum to the bryophyte is (2-5): 2;
the content of polysaccharide in the extract containing algae essence is 40-60%.
2. The method of preparing an extract containing algae essence according to claim 1, wherein the algae extract has a relative density of 1.3 to 1.7.
3. The method of claim 1, wherein the mass ratio of the yeast fermentation medium to the algae extract is 10: (9-15).
4. The method for preparing the extract containing algae essence as claimed in claim 1, wherein the microwave is heated to 45-60 ℃ and the microwave power is 1000-1200W.
5. The method as claimed in claim 1, wherein the fermentation conditions in step S2 are temperature 28-34 deg.C, rotation speed 100-150r/min, and ventilation 2-5 VVm.
6. The method for preparing an extract containing algae essence according to claim 1, wherein the volume ratio of the fermentation liquid to the absolute ethyl alcohol is 1: (1-3).
7. A moisturizing lotion characterized by containing 4 to 10wt% of the algae essence-containing extract according to claim 1.
8. The moisturizing emulsion according to claim 7, characterized in that it comprises the following components in mass fraction: 20200.5-1.2% of carbomer resin, 0.1-1% of dipotassium glycyrrhizinate, 0.2-0.5% of water-soluble vitamin E, 4-10% of algae essence-containing extract in claim 1, 0.5-1.5% of shea butter, 2-4% of glycerol, 1-2% of butanediol, 0.02-0.05% of disodium EDTA, 0.01-0.03% of phenoxyethanol and the balance of purified water.
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| CN108653059A (en) * | 2018-06-20 | 2018-10-16 | 齐鲁工业大学 | A kind of probiotics fermention prepares method and the application of the fermentation of seaweed liquid in cosmetics of fermentation of seaweed liquid |
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